LOCUS       pET-MhTPS-6xHis         6758 bp    DNA     circular UNA 10-NOV-2022
DEFINITION  Expression of MhTPS-6xHis in bacterial of cells.
ACCESSION   urn.local...i-fj419cw
VERSION     urn.local...i-fj419cw
KEYWORDS    .
SOURCE      
  ORGANISM  .
COMMENT     ##Imported_From-START##
            Path     :: /Users/annonaceae/Downloads
            Filename :: addgene-plasmid-62939-sequence-106328.dna
            ##Imported_From-END##
            ##SnapGene_Notes-START##
            Confirmed Experimentally :: 0
            Transformed Into         :: unspecified
            Sequence Document Type   :: UNA
            SnapGene UUID            :: 4e952866-34e1-422d-af2d-831329482aff
            Use Custom Sequence Name :: 1
            Sequence Type            :: Synthetic
            ##SnapGene_Notes-END##
FEATURES             Location/Qualifiers
     protein_bind    3..27
                     /bound_moiety="<html><body><i>lac</i> repressor encoded by
                     <i>lacI</i></body></html>"
                     /note="<html><body>The <i>lac</i> repressor binds to the
                     <i>lac</i> operator to inhibit transcription in <i>E.
                     coli</i>. This inhibition can be relieved by adding
                     lactose or isopropyl-β-D-thiogalactopyranoside
                     (IPTG).</body></html>"
                     /label="lac operator"
     misc_feature    30..35
                     /Recognition_pattern="T^CTAGA"
                     /Methylation="Dam:blocked"
                     /label="XbaI"
                     /note="Geneious type: restriction site"
     CDS             48..1655
                     /translation="MRRSANYRPSIWDYDFIKSLSGDYESDTSAKRMEALKEQIRPSF
                     HGIKASESAELIDNLQRLGIGHLFLKEMKEALDSMSINDNNLGMEGDLHATALKFRLL
                     RQHGYMISQDVFNNFRDDEGNFKQSILMDIKGTLSLYEASYLSFQGEIVLDKAQAFTK
                     KHLKDLKGEANTNLQRLIDHALELPLHWRCPRPESKWYIETYERTHNTNPVILELAKL
                     NFNMEQSILQKDLQDVARWWQKLGLQRKLSFCRDRMVECFLFGLFMVDDAQFEYGRKE
                     VSKVNQLVTTIDDIYDVYGSLDELELFTNAVERWDLEAMELLPDYMKLCFLALYNTAN
                     EVAYRVLEEQGCNALPYIKKAWADLCKGYLVEAKWYNNKYTPTVDEYLEHSWRTISTH
                     AALTYIYFARQRENITKDELYVLENYGDLIRHCCLIFRLNDDLGTSKDEMARGDSPKA
                     IQCYMHEKGISESAAREEIAVVISETWKKMNEVCMNSTTFSENYIKAVKNTARSSHYV
                     YQFGDGFGVVCNDMKDRILGLLAEPIPL"
                     /label="MhTPS"
     misc_feature    1662..1667
                     /Recognition_pattern="G^GATCC"
                     /Methylation="Dam:cut, Dcm:cut, CpG:cut"
                     /label="BamHI"
                     /note="Geneious type: restriction site"
     CDS             1668..1685
                     /translation="HHHHHH"
                     /transl_table=1
                     /product="<html><body>6xHis affinity tag</body></html>"
                     /codon_start=1
                     /label="6xHis"
     misc_feature    1690..1699
                     /Recognition_pattern="GACNN^NNGTC"
                     /Methylation="Mixed Methylation"
                     /label="PshAI, BoxI, BstPAI"
                     /note="Geneious type: restriction site"
     misc_feature    1691..1698
                     /Recognition_pattern="VC^TCGAGB"
                     /Methylation="CpG:some impaired, EcoBI:cut, EcoKI:cut"
                     /label="PspXI"
                     /note="Geneious type: restriction site"
     misc_feature    1692..1697
                     /Recognition_pattern="C^YCGRG"
                     /Methylation="Mixed Methylation"
                     /label="AvaI, Ama87I, BmeT110I, BsiHKCI, BsoBI, Eco88I"
                     /note="Geneious type: restriction site"
     misc_feature    1692..1697
                     /Recognition_pattern="C^TCGAG"
                     /Methylation="Mixed Methylation"
                     /label="XhoI, PaeR7I, Sfr274I, SlaI, StrI"
                     /note="Geneious type: restriction site"
     CDS             1704..1748
                     /translation="KETAAAKFERQHMDS"
                     /transl_table=1
                     /product="<html><body>affinity and epitope tag derived
                     from pancreatic ribonuclease A</body></html>"
                     /codon_start=1
                     /label="S-Tag"
     misc_feature    1733..1743
                     /Recognition_pattern="CCANNNN^NTGG"
                     /Methylation="Mixed Methylation"
                     /label="PflMI, AccB7I, BasI, Van91I"
                     /note="Geneious type: restriction site"
     misc_feature    1748..1753
                     /Recognition_pattern="GT^MKAC"
                     /Methylation="Mixed Methylation"
                     /label="AccI, FblI, XmiI"
                     /note="Geneious type: restriction site"
     misc_feature    1763..1770
                     /Recognition_pattern="TTAAT^TAA"
                     /Methylation="EcoKI:cut"
                     /label="PacI"
                     /note="Geneious type: restriction site"
     misc_feature    1771..1776
                     /Recognition_pattern="C^CTAGG"
                     /label="AvrII, AspA2I, BlnI, XmaJI"
                     /note="Geneious type: restriction site"
     misc_feature    1771..1776
                     /Recognition_pattern="C^CWWGG"
                     /label="StyI, BssT1I, Eco130I, EcoT14I, ErhI"
                     /note="Geneious type: restriction site"
     primer_bind     complement(1786..1804)
                     /Tm=56
                     /Description="T7 terminator, reverse primer"
                     /label="T7 Term"
     misc_feature    1788..1794
                     /Recognition_pattern="GC^TNAGC"
                     /Methylation="Mixed Methylation"
                     /label="BlpI, Bpu1102I, Bsp1720I"
                     /note="Geneious type: restriction site"
     terminator      1800..1847
                     /note="<html><body>transcription terminator for
                     bacteriophage T7 RNA polymerase</body></html>"
                     /label="T7 terminator"
     misc_feature    1811..1816
                     /Recognition_pattern="C^CWWGG"
                     /label="StyI, BssT1I, Eco130I, EcoT14I, ErhI"
                     /note="Geneious type: restriction site"
     misc_feature    1866..1871
                     /Recognition_pattern="T^CCGGA"
                     /Methylation="Mixed Methylation"
                     /label="AccIII, Aor13HI, BseAI, Bsp13I, BspEI, Kpn2I,
                     MroI"
                     /note="Geneious type: restriction site"
     rep_origin      1884..2339
                     /direction="RIGHT"
                     /note="<html><body>f1 bacteriophage origin of replication;
                     arrow indicates direction of (+) strand
                     synthesis</body></html>"
                     /label="f1 ori"
     primer_bind     complement(1971..1990)
                     /Tm=57
                     /Description="F1 origin, reverse primer"
                     /label="F1ori-R"
     misc_feature    2009..2014
                     /Recognition_pattern="GCC^GGC"
                     /Methylation="Mixed Methylation"
                     /label="NaeI, PdiI"
                     /note="Geneious type: restriction site"
     misc_feature    2009..2014
                     /Recognition_pattern="G^CCGGC"
                     /Methylation="Mixed Methylation"
                     /label="KroI, MroNI, NgoMIV"
                     /note="Geneious type: restriction site"
     misc_feature    2112..2120
                     /Recognition_pattern="CACNNN^GTG"
                     /Methylation="Mixed Methylation"
                     /label="DraIII, AdeI"
                     /note="Geneious type: restriction site"
     misc_feature    2161..2173
                     /Recognition_pattern="(7/12)GAACNNNNNNTCC(12/7)"
                     /label="AloI"
                     /note="Geneious type: restriction site"
     misc_feature    2161..2171
                     /Recognition_pattern="(9/12)ACNNNNNCTCC(10/7)"
                     /Methylation="Dcm:cut, CpG:cut"
                     /label="BsaXI"
                     /note="Geneious type: restriction site"
     primer_bind     2181..2202
                     /Tm=57
                     /Description="F1 origin, forward primer"
                     /label="F1ori-F"
     misc_feature    2240..2245
                     /Recognition_pattern="TTA^TAA"
                     /Methylation="Mixed Methylation"
                     /label="PsiI, AanI"
                     /note="Geneious type: restriction site"
     misc_feature    2320..2325
                     /Recognition_pattern="AAT^ATT"
                     /Methylation="EcoBI:cut"
                     /label="SspI"
                     /note="Geneious type: restriction site"
     CDS             join(complement(3246..3314),complement(2454..3245))
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVG
                     YIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLV
                     EYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTR
                     LDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGP
                     LLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQI
                     AEIGASLIKHW*"
                     /transl_table=1
                     /product="<html><body>β-lactamase</body></html>"
                     /gene="<html><body><i>bla</i></body></html>"
                     /codon_start=1
                     /note="<html><body>confers resistance to ampicillin,
                     carbenicillin, and related antibiotics</body></html>"
                     /label="AmpR"
     misc_feature    2522..2532
                     /Recognition_pattern="GACNNN^NNGTC"
                     /Methylation="Mixed Methylation"
                     /label="Eam1105I, AhdI, BmeRI, DriI"
                     /note="Geneious type: restriction site"
     misc_feature    2594..2599
                     /Recognition_pattern="GGTCTC(1/5)"
                     /Methylation="Mixed Methylation"
                     /label="Eco31I, BsaI, Bso31I"
                     /note="Geneious type: restriction site"
     misc_feature    2641..2651
                     /Recognition_pattern="GCCNNNN^NGGC"
                     /Methylation="Dcm:cut, CpG:variable"
                     /label="BglI"
                     /note="Geneious type: restriction site"
     misc_feature    2650..2655
                     /Recognition_pattern="GCCGAG(21/19)"
                     /Methylation="CpG:cut"
                     /label="NmeAIII"
                     /note="Geneious type: restriction site"
     misc_feature    2747..2752
                     /Recognition_pattern="TGC^GCA"
                     /Methylation="Mixed Methylation"
                     /label="Acc16I, FspI, NsbI"
                     /note="Geneious type: restriction site"
     misc_feature    2894..2899
                     /Recognition_pattern="CGAT^CG"
                     /Methylation="Mixed Methylation"
                     /label="PvuI, BpvUI, MvrI, Ple19I"
                     /note="Geneious type: restriction site"
     misc_feature    3005..3010
                     /Recognition_pattern="AGT^ACT"
                     /Methylation="Mixed Methylation"
                     /label="ScaI, AssI, BmcAI, ZrmI"
                     /note="Geneious type: restriction site"
     misc_feature    3005..3010
                     /Recognition_pattern="W^GTACW"
                     /label="TatI"
                     /note="Geneious type: restriction site"
     primer_bind     3077..3096
                     /Tm=58
                     /Description="Ampicillin resistance gene, reverse primer"
                     /label="Amp-R"
     misc_feature    3191..3196
                     /Recognition_pattern="CACGAG(-5/-1)"
                     /Methylation="Mixed Methylation"
                     /label="BauI, BssSI, Bst2BI"
                     /note="Geneious type: restriction site"
     promoter        complement(3315..3407)
                     /gene="<html><body><i>bla</i></body></html>"
                     /label="AmpR promoter"
     rep_origin      3488..4076
                     /direction="RIGHT"
                     /note="<html><body>high-copy-number ColE1/pMB1/pBR322/pUC
                     origin of replication</body></html>"
                     /label="ori"
     misc_feature    3718..3726
                     /Recognition_pattern="CAGNNN^CTG"
                     /Methylation="Mixed Methylation"
                     /label="AlwNI, CaiI, PstNI"
                     /note="Geneious type: restriction site"
     misc_feature    3959..3964
                     /Recognition_pattern="CACGAG(-5/-1)"
                     /Methylation="Mixed Methylation"
                     /label="BauI, BssSI, Bst2BI"
                     /note="Geneious type: restriction site"
     primer_bind     3977..3996
                     /Tm=56
                     /Description="pBR322 origin, forward primer"
                     /label="pBR322ori-F"
     misc_feature    4132..4137
                     /Recognition_pattern="A^CATGT"
                     /Methylation="Mixed Methylation"
                     /label="PciI, PscI"
                     /note="Geneious type: restriction site"
     primer_bind     4230..4247
                     /Tm=58
                     /Description="L4440 vector, forward primer"
                     /label="L4440"
     misc_feature    4254..4260
                     /Recognition_pattern="GCTCTTC(1/4)"
                     /Methylation="Mixed Methylation"
                     /label="SapI, BspQI, LguI, PciSI"
                     /note="Geneious type: restriction site"
     misc_feature    4262..4404
                     /note="<html><body>basis of mobility region from
                     pBR322</body></html>"
                     /label="bom"
     misc_feature    4328..4333
                     /Recognition_pattern="W^GTACW"
                     /label="TatI"
                     /note="Geneious type: restriction site"
     primer_bind     complement(4330..4349)
                     /Tm=54
                     /Description="pRS vectors, use to sequence yeast
                     selectable marker"
                     /label="pRS-marker"
     misc_feature    4363..4368
                     /Recognition_pattern="GT^MKAC"
                     /Methylation="Mixed Methylation"
                     /label="AccI, FblI, XmiI"
                     /note="Geneious type: restriction site"
     misc_feature    4363..4368
                     /Recognition_pattern="GTA^TAC"
                     /Methylation="Mixed Methylation"
                     /label="BssNAI, Bst1107I, BstZ17I"
                     /note="Geneious type: restriction site"
     misc_feature    4387..4395
                     /Recognition_pattern="GACN^NNGTC"
                     /Methylation="Mixed Methylation"
                     /label="Tth111I, PflFI, PsyI"
                     /note="Geneious type: restriction site"
     misc_feature    4489..4495
                     /Recognition_pattern="T^CCNGGA"
                     /label="PfoI"
                     /note="Geneious type: restriction site"
     primer_bind     4490..4512
                     /Tm=66
                     /Description="pGEX vectors, reverse primer"
                     /label="pGEX 3'"
     CDS             complement(4506..4697)
                     /translation="VTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDH
                     ADELYRSCLARFGDDGENL*"
                     /transl_table=1
                     /product="<html><body>Rop protein, which maintains
                     plasmids at low copy number</body></html>"
                     /gene="<html><body><i>rop</i></body></html>"
                     /codon_start=1
                     /label="rop"
     misc_feature    4880..4885
                     /Recognition_pattern="AGC^GCT"
                     /Methylation="Mixed Methylation"
                     /label="Eco47III, AfeI, Aor51HI"
                     /note="Geneious type: restriction site"
     misc_feature    4923..4934
                     /Recognition_pattern="(10/12)GCANNNNNNTGC(12/10)"
                     /label="AlfI"
                     /note="Geneious type: restriction site"
     misc_feature    4943..4948
                     /Recognition_pattern="T^CCGGA"
                     /Methylation="Mixed Methylation"
                     /label="AccIII, Aor13HI, BseAI, Bsp13I, BspEI, Kpn2I,
                     MroI"
                     /note="Geneious type: restriction site"
     misc_feature    5026..5032
                     /Recognition_pattern="CCTNAGC(-5/-2)"
                     /Methylation="CpG:cut, EcoBI:cut"
                     /label="Bpu10I"
                     /note="Geneious type: restriction site"
     misc_feature    5126..5132
                     /Recognition_pattern="RG^GWCCY"
                     /Methylation="Mixed Methylation"
                     /label="PpuMI, Psp5II, PspPPI"
                     /note="Geneious type: restriction site"
     misc_feature    5144..5155
                     /Recognition_pattern="(10/12)GCANNNNNNTGC(12/10)"
                     /label="AlfI"
                     /note="Geneious type: restriction site"
     CDS             complement(5269..6351)
                     /translation="VKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAE
                     LNYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERS
                     GVEACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSII
                     FSHEDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREG
                     DWSAMSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDT
                     EDSSCYIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNT
                     QTASPRALADSLMQLARQVSRLESGQ*"
                     /transl_table=1
                     /product="<html><body><i>lac</i> repressor</body></html>"
                     /gene="<html><body><i>lacI</i></body></html>"
                     /codon_start=1
                     /note="<html><body>The <i>lac</i> repressor binds to the
                     <i>lac</i> operator to inhibit transcription in <i>E.
                     coli. </i>This inhibition can be relieved by adding
                     lactose or isopropyl-β-D-thiogalactopyranoside
                     (IPTG).</body></html>"
                     /label="lacI"
     misc_feature    5336..5346
                     /Recognition_pattern="(9/12)ACNNNNNCTCC(10/7)"
                     /Methylation="Dcm:cut, CpG:cut"
                     /label="BsaXI"
                     /note="Geneious type: restriction site"
     misc_feature    5492..5497
                     /Recognition_pattern="GTT^AAC"
                     /Methylation="Mixed Methylation"
                     /label="HpaI, KspAI"
                     /note="Geneious type: restriction site"
     misc_feature    5492..5497
                     /Recognition_pattern="GTY^RAC"
                     /Methylation="Mixed Methylation"
                     /label="HindII, HincII"
                     /note="Geneious type: restriction site"
     misc_feature    5585..5590
                     /Recognition_pattern="G^CGCGC"
                     /Methylation="Mixed Methylation"
                     /label="BsePI, BssHII, PauI, PteI"
                     /note="Geneious type: restriction site"
     misc_feature    5771..5776
                     /Recognition_pattern="GCCGAG(21/19)"
                     /Methylation="CpG:cut"
                     /label="NmeAIII"
                     /note="Geneious type: restriction site"
     misc_feature    5789..5794
                     /Recognition_pattern="GGGCC^C"
                     /Methylation="Dcm:some blocked, CpG:some blocked"
                     /label="ApaI"
                     /note="Geneious type: restriction site"
     misc_feature    5789..5794
                     /Recognition_pattern="G^GGCCC"
                     /Methylation="Mixed Methylation"
                     /label="Bsp120I, PspOMI"
                     /note="Geneious type: restriction site"
     misc_feature    5814..5820
                     /Recognition_pattern="G^GTNACC"
                     /Methylation="Mixed Methylation"
                     /label="BstEII, BstPI, Eco91I, EcoO65I, PspEI"
                     /note="Geneious type: restriction site"
     misc_feature    5982..5987
                     /Recognition_pattern="T^GATCA"
                     /Methylation="Mixed Methylation"
                     /label="BclI, FbaI, Ksp22I"
                     /note="Geneious type: restriction site"
     misc_feature    5996..6001
                     /Recognition_pattern="A^CGCGT"
                     /Methylation="CpG:blocked, EcoBI:variable, EcoKI:variable"
                     /label="MluI"
                     /note="Geneious type: restriction site"
     primer_bind     6313..6332
                     /Tm=58
                     /Description="LacI, reverse primer"
                     /label="LacI-R"
     misc_feature    6314..6324
                     /Recognition_pattern="GCANNNN^NTGC"
                     /Methylation="CpG:variable, EcoBI:blocked"
                     /label="BstAPI"
                     /note="Geneious type: restriction site"
     promoter        complement(6352..6429)
                     /gene="<html><body><i>lacI</i></body></html>"
                     /note="<html><body><br></body></html>"
                     /label="lacI promoter"
     misc_feature    6415..6425
                     /Recognition_pattern="CCANNNN^NTGG"
                     /Methylation="Mixed Methylation"
                     /label="PflMI, AccB7I, BasI, Van91I"
                     /note="Geneious type: restriction site"
     misc_feature    6428..6434
                     /Recognition_pattern="T^CCNGGA"
                     /label="PfoI"
                     /note="Geneious type: restriction site"
     misc_feature    6460..6470
                     /Recognition_pattern="CCTNN^NNNAGG"
                     /Methylation="Mixed Methylation"
                     /label="EcoNI, BstENI, XagI"
                     /note="Geneious type: restriction site"
     misc_feature    6525..6530
                     /Recognition_pattern="GCATG^C"
                     /Methylation="Mixed Methylation"
                     /label="SphI, PaeI"
                     /note="Geneious type: restriction site"
     misc_feature    6559..6564
                     /Recognition_pattern="C^CRYGG"
                     /Methylation="Mixed Methylation"
                     /label="BstDSI, BtgI"
                     /note="Geneious type: restriction site"
     misc_feature    6593..6598
                     /Recognition_pattern="AGC^GCT"
                     /Methylation="Mixed Methylation"
                     /label="Eco47III, AfeI, Aor51HI"
                     /note="Geneious type: restriction site"
     primer_bind     6635..6654
                     /Tm=56
                     /Description="pBR322 vectors, tet region, downstream of
                     BamHI, reverse primer"
                     /label="pBRrevBam"
     misc_feature    6676..6683
                     /Recognition_pattern="CR^CCGGYG"
                     /Methylation="CpG:variable, EcoBI:some blocked, EcoKI:some
                     blocked"
                     /label="SgrAI"
                     /note="Geneious type: restriction site"
     misc_feature    6686..6691
                     /Recognition_pattern="GCC^GGC"
                     /Methylation="Mixed Methylation"
                     /label="NaeI, PdiI"
                     /note="Geneious type: restriction site"
     misc_feature    6686..6691
                     /Recognition_pattern="G^CCGGC"
                     /Methylation="Mixed Methylation"
                     /label="KroI, MroNI, NgoMIV"
                     /note="Geneious type: restriction site"
     misc_feature    6720..6725
                     /Recognition_pattern="AT^CGAT"
                     /Methylation="Mixed Methylation"
                     /label="ClaI, Bsa29I, BseCI, BshVI, BspDI, Bsu15I"
                     /note="Geneious type: restriction site"
     promoter        6742..2
                     /note="<html><body>promoter for bacteriophage T7 RNA
                     polymerase</body></html>"
                     /label="T7 promoter"
     primer_bind     6742..3
                     /Tm=49
                     /Description="T7 promoter, forward primer"
                     /label="T7"
ORIGIN      
        1 ggggaattgt gagcggataa caattcccct ctagaaggag gtataatatg cggagatctg
       61 ctaattatcg acctagcatt tgggactatg attttatcaa atcactcagt ggtgattacg
      121 agtctgatac gtccgcaaaa cggatggaag cactgaagga gcagataagg ccgtcgttcc
      181 atgggatcaa agcatcagaa agcgcggagc taattgacaa cctccaaaga ttgggaatag
      241 gtcatctatt tttaaaggag atgaaggaag cactagactc aatgtccatc aatgataata
      301 atctggggat ggaaggggac ctccatgcta cggccctcaa gttcaggcta cttagacaac
      361 atggttacat gatttcacaa gatgtgttca ataacttcag agatgacgag ggtaatttta
      421 agcaaagcat tttgatggac ataaagggaa cgctgagtct atatgaagct tcatatcttt
      481 ctttccaagg tgaaatcgtc ttagataagg cccaagcttt caccaaaaaa cacctcaagg
      541 atctaaaagg ggaagcaaac acaaaccttc aaaggttaat agatcatgca ttggagcttc
      601 cattgcactg gaggtgtcct aggccagagt ccaagtggta catagagaca tatgaaagaa
      661 cgcacaacac taatcctgta atacttgaat tggccaagct gaacttcaac atggagcagt
      721 ctatcctaca aaaagacttg caggacgtgg caagatggtg gcagaagtta ggccttcaaa
      781 ggaagttgag cttctgcaga gaccggatgg tggagtgttt ccttttcggt ttgttcatgg
      841 tagacgatgc ccagttcgaa tacggaagaa aagaagtttc aaaagtaaat cagctagtaa
      901 caacgattga tgatatttat gatgtttatg gttcattgga tgagctagag ctattcacaa
      961 acgctgttga gagatgggat ctcgaggcaa tggaattact tccagattat atgaagctgt
     1021 gctttctagc tctttacaac accgctaatg aagtagctta tcgtgtctta gaggaacaag
     1081 gatgcaatgc actgccatac ataaagaaag cgtgggcaga tttatgtaaa ggatatttag
     1141 tggaggcaaa atggtataac aacaaatata caccaacagt cgatgagtat cttgagcatt
     1201 cttggcgtac aattagtact catgctgcgc taacctacat ttattttgca cgacaacgag
     1261 aaaatataac gaaagatgaa ttatacgtct tagagaatta tggagatctt atccgccact
     1321 gctgtctgat tttccggcta aatgatgatc taggaacttc aaaagatgag atggctagag
     1381 gtgattcccc aaaagccatc caatgctaca tgcacgaaaa gggtatttct gaatcagctg
     1441 cgcgtgaaga aatagctgtt gtgatcagtg agacatggaa gaagatgaac gaagtatgca
     1501 tgaattctac gactttctct gaaaactata tcaaggctgt aaagaacact gcacggagta
     1561 gccattatgt gtaccagttt ggagatgggt ttggagtggt atgcaatgac atgaaggacc
     1621 gaatcctggg gctgttggct gaaccaatac cacttgacgg cggatcccat caccaccacc
     1681 atcactaatg actcgagtct ggtaaagaaa ccgctgctgc gaaatttgaa cgccagcaca
     1741 tggactcgtc tactagcgca gcttaattaa cctaggctgc tgccaccgct gagcaataac
     1801 tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa
     1861 ctatatccgg attggcgaat gggacgcgcc ctgtagcggc gcattaagcg cggcgggtgt
     1921 ggtggttacg cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc
     1981 tttcttccct tcctttctcg ccacgttcgc cggctttccc cgtcaagctc taaatcgggg
     2041 gctcccttta gggttccgat ttagtgcttt acggcacctc gaccccaaaa aacttgatta
     2101 gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt
     2161 ggagtccacg ttctttaata gtggactctt gttccaaact ggaacaacac tcaaccctat
     2221 ctcggtctat tcttttgatt tataagggat tttgccgatt tcggcctatt ggttaaaaaa
     2281 tgagctgatt taacaaaaat ttaacgcgaa ttttaacaaa atattaacgt ttacaatttc
     2341 tggcggcacg atggcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa
     2401 aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa
     2461 tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc
     2521 tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct
     2581 gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca
     2641 gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt
     2701 aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt
     2761 gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc
     2821 ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc
     2881 tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt
     2941 atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact
     3001 ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc
     3061 ccggcgtcaa tacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt
     3121 ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg
     3181 atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct
     3241 gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa
     3301 tgttgaatac tcatactctt cctttttcaa tcatgattga agcatttatc agggttattg
     3361 tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gtcatgacca
     3421 aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag
     3481 gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac
     3541 cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa
     3601 ctggcttcag cagagcgcag ataccaaata ctgtccttct agtgtagccg tagttaggcc
     3661 accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag
     3721 tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac
     3781 cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc
     3841 gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc
     3901 ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca
     3961 cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc
     4021 tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg
     4081 ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct
     4141 ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata
     4201 ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc
     4261 gcctgatgcg gtattttctc cttacgcatc tgtgcggtat ttcacaccgc atatatggtg
     4321 cactctcagt acaatctgct ctgatgccgc atagttaagc cagtatacac tccgctatcg
     4381 ctacgtgact gggtcatggc tgcgccccga cacccgccaa cacccgctga cgcgccctga
     4441 cgggcttgtc tgctcccggc atccgcttac agacaagctg tgaccgtctc cgggagctgc
     4501 atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga ggcagctgcg gtaaagctca
     4561 tcagcgtggt cgtgaagcga ttcacagatg tctgcctgtt catccgcgtc cagctcgttg
     4621 agtttctcca gaagcgttaa tgtctggctt ctgataaagc gggccatgtt aagggcggtt
     4681 ttttcctgtt tggtcactga tgcctccgtg taagggggat ttctgttcat gggggtaatg
     4741 ataccgatga aacgagagag gatgctcacg atacgggtta ctgatgatga acatgcccgg
     4801 ttactggaac gttgtgaggg taaacaactg gcggtatgga tgcggcggga ccagagaaaa
     4861 atcactcagg gtcaatgcca gcgcttcgtt aatacagatg taggtgttcc acagggtagc
     4921 cagcagcatc ctgcgatgca gatccggaac ataatggtgc agggcgctga cttccgcgtt
     4981 tccagacttt acgaaacacg gaaaccgaag accattcatg ttgttgctca ggtcgcagac
     5041 gttttgcagc agcagtcgct tcacgttcgc tcgcgtatcg gtgattcatt ctgctaacca
     5101 gtaaggcaac cccgccagcc tagccgggtc ctcaacgaca ggagcacgat catgctagtc
     5161 atgccccgcg cccaccggaa ggagctgact gggttgaagg ctctcaaggg catcggtcga
     5221 gatcccggtg cctaatgagt gagctaactt acattaattg cgttgcgctc actgcccgct
     5281 ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa tcggccaacg cgcggggaga
     5341 ggcggtttgc gtattgggcg ccagggtggt ttttcttttc accagtgaga cgggcaacag
     5401 ctgattgccc ttcaccgcct ggccctgaga gagttgcagc aagcggtcca cgctggtttg
     5461 ccccagcagg cgaaaatcct gtttgatggt ggttaacggc gggatataac atgagctgtc
     5521 ttcggtatcg tcgtatccca ctaccgagat gtccgcacca acgcgcagcc cggactcggt
     5581 aatggcgcgc attgcgccca gcgccatctg atcgttggca accagcatcg cagtgggaac
     5641 gatgccctca ttcagcattt gcatggtttg ttgaaaaccg gacatggcac tccagtcgcc
     5701 ttcccgttcc gctatcggct gaatttgatt gcgagtgaga tatttatgcc agccagccag
     5761 acgcagacgc gccgagacag aacttaatgg gcccgctaac agcgcgattt gctggtgacc
     5821 caatgcgacc agatgctcca cgcccagtcg cgtaccgtct tcatgggaga aaataatact
     5881 gttgatgggt gtctggtcag agacatcaag aaataacgcc ggaacattag tgcaggcagc
     5941 ttccacagca atggcatcct ggtcatccag cggatagtta atgatcagcc cactgacgcg
     6001 ttgcgcgaga agattgtgca ccgccgcttt acaggcttcg acgccgcttc gttctaccat
     6061 cgacaccacc acgctggcac ccagttgatc ggcgcgagat ttaatcgccg cgacaatttg
     6121 cgacggcgcg tgcagggcca gactggaggt ggcaacgcca atcagcaacg actgtttgcc
     6181 cgccagttgt tgtgccacgc ggttgggaat gtaattcagc tccgccatcg ccgcttccac
     6241 tttttcccgc gttttcgcag aaacgtggct ggcctggttc accacgcggg aaacggtctg
     6301 ataagagaca ccggcatact ctgcgacatc gtataacgtt actggtttca cattcaccac
     6361 cctgaattga ctctcttccg ggcgctatca tgccataccg cgaaaggttt tgcgccattc
     6421 gatggtgtcc gggatctcga cgctctccct tatgcgactc ctgcattagg aagcagccca
     6481 gtagtaggtt gaggccgttg agcaccgccg ccgcaaggaa tggtgcatgc aaggagatgg
     6541 cgcccaacag tcccccggcc acggggcctg ccaccatacc cacgccgaaa caagcgctca
     6601 tgagcccgaa gtggcgagcc cgatcttccc catcggtgat gtcggcgata taggcgccag
     6661 caaccgcacc tgtggcgccg gtgatgccgg ccacgatgcg tccggcgtag aggatcgaga
     6721 tcgatctcga tcccgcgaaa ttaatacgac tcactata
//
