LOCUS       pTarg_cLap__2__D        5404 bp    DNA        circular     16-NOV-2022
DEFINITION  
ACCESSION   
VERSION     
SOURCE      
  ORGANISM  
COMMENT     
COMMENT     ApEinfo:methylated:1
FEATURES             Location/Qualifiers
     primer_bind     2659..2674
                     /vntifkey="28"
                     /locus_tag="M13rev"
                     /label="M13rev"
                     /ApEinfo_label="M13rev"
                     /ApEinfo_fwdcolor="pink"
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    754..1038
                     /locus_tag="NGFP-exon1"
                     /label="NGFP-exon1"
                     /ApEinfo_label="NGFP-exon1"
                     /ApEinfo_fwdcolor="#2bff27"
                     /ApEinfo_revcolor="#2bff27"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    342..360
                     /locus_tag="CRISPR"
                     /label="CRISPR"
                     /ApEinfo_label="CRISPR"
                     /ApEinfo_fwdcolor="#66ff66"
                     /ApEinfo_revcolor="#ff6666"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    1039..1470
                     /locus_tag="NGFP-exon2"
                     /label="NGFP-exon2"
                     /ApEinfo_label="NGFP-exon2"
                     /ApEinfo_fwdcolor="#17ff51"
                     /ApEinfo_revcolor="#17ff51"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    337..340
                     /locus_tag="Overhang 3"
                     /label="Overhang 3"
                     /ApEinfo_label="Overhang 3"
                     /ApEinfo_fwdcolor="#ff80c0"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    1473..1531
                     /locus_tag="P2A"
                     /label="P2A"
                     /ApEinfo_label="P2A"
                     /ApEinfo_fwdcolor="#ff8000"
                     /ApEinfo_revcolor="#ff0000"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    101..332
                     /locus_tag="hU6"
                     /label="hU6"
                     /ApEinfo_label="hU6"
                     /ApEinfo_fwdcolor="#0000ff"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    697..732
                     /locus_tag="PreScission (hum.adopt)"
                     /label="PreScission (hum.adopt)"
                     /ApEinfo_label="PreScission (hum.adopt)"
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="cyan"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     primer_bind     3..24
                     /vntifkey="28"
                     /locus_tag="M13\for"
                     /label="M13\for"
                     /ApEinfo_label="M13\for"
                     /ApEinfo_fwdcolor="pink"
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    586..606
                     /locus_tag="TEV(hum.adopt)"
                     /label="TEV(hum.adopt)"
                     /ApEinfo_label="TEV(hum.adopt)"
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="cyan"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     polyA_signal    64..65
                     /vntifkey="25"
                     /locus_tag="bpA"
                     /label="bpA"
                     /ApEinfo_label="bpA"
                     /ApEinfo_fwdcolor="pink"
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    complement(2562..2564)
                     /locus_tag="Pam"
                     /label="Pam"
                     /ApEinfo_label="Pam"
                     /ApEinfo_fwdcolor="#999999"
                     /ApEinfo_revcolor="#999999"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     primer_bind     complement(2622..2641)
                     /locus_tag="T3"
                     /label="T3"
                     /ApEinfo_label="T3"
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    complement(2565..2584)
                     /locus_tag="CRISPR(1)"
                     /label="CRISPR(1)"
                     /ApEinfo_label="CRISPR"
                     /ApEinfo_fwdcolor="#66ff66"
                     /ApEinfo_revcolor="#ff6666"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    72..89
                     /vntifkey="21"
                     /locus_tag="I-Sce"
                     /label="I-Sce"
                     /ApEinfo_label="I-Sce"
                     /ApEinfo_fwdcolor="#cccccc"
                     /ApEinfo_revcolor="#cccccc"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     primer_bind     complement(2659..2679)
                     /locus_tag="M13-rev"
                     /label="M13-rev"
                     /ApEinfo_label="M13-rev"
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     primer_bind     7..24
                     /locus_tag="M13-fwd"
                     /label="M13-fwd"
                     /ApEinfo_label="M13-fwd"
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     primer_bind     33..53
                     /locus_tag="T7"
                     /label="T7"
                     /ApEinfo_label="T7"
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     rep_origin      complement(3047..3729)
                     /locus_tag="ColE1 origin"
                     /label="ColE1 origin"
                     /ApEinfo_label="ColE1 origin"
                     /ApEinfo_fwdcolor="gray50"
                     /ApEinfo_revcolor="gray50"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     rep_origin      complement(4947..5253)
                     /locus_tag="F1 ori"
                     /label="F1 ori"
                     /ApEinfo_label="F1 ori"
                     /ApEinfo_fwdcolor="gray50"
                     /ApEinfo_revcolor="gray50"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     CDS             complement(5272..5340)
                     /locus_tag="LacZ alpha"
                     /label="LacZ alpha"
                     /ApEinfo_label="LacZ alpha"
                     /ApEinfo_fwdcolor="#6495ed"
                     /ApEinfo_revcolor="#6495ed"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_binding    complement(2685..2707)
                     /locus_tag="LacO"
                     /label="LacO"
                     /ApEinfo_label="LacO"
                     /ApEinfo_fwdcolor="#6495ed"
                     /ApEinfo_revcolor="#6495ed"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     CDS             complement(3827..4486)
                     /locus_tag="AmpR"
                     /label="AmpR"
                     /ApEinfo_label="AmpR"
                     /ApEinfo_fwdcolor="yellow"
                     /ApEinfo_revcolor="yellow"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    361..364
                     /locus_tag="Overhang 5"
                     /label="Overhang 5"
                     /ApEinfo_label="Overhang 5"
                     /ApEinfo_fwdcolor="#ff80c0"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     polyA_signal    2594..2595
                     /vntifkey="25"
                     /locus_tag="bpA(1)"
                     /label="bpA(1)"
                     /ApEinfo_label="bpA"
                     /ApEinfo_fwdcolor="pink"
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     protein_bind    547..570
                     /locus_tag="FLAG(hum.adopt)"
                     /label="FLAG(hum.adopt)"
                     /ApEinfo_label="FLAG(hum.adopt)"
                     /ApEinfo_fwdcolor="#ff2834"
                     /ApEinfo_revcolor="#ff2834"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     protein_bind    622..663
                     /locus_tag="S-pept(hum.adopt)"
                     /label="S-pept(hum.adopt)"
                     /ApEinfo_label="S-pept(hum.adopt)"
                     /ApEinfo_fwdcolor="#009a9a"
                     /ApEinfo_revcolor="#009a9a"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    complement(498..517)
                     /locus_tag="CRISPR(2)"
                     /label="CRISPR(2)"
                     /ApEinfo_label="CRISPR"
                     /ApEinfo_fwdcolor="#66ff66"
                     /ApEinfo_revcolor="#ff6666"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    1534..2556
                     /locus_tag="Hygro"
                     /label="Hygro"
                     /ApEinfo_label="Hygro"
                     /ApEinfo_fwdcolor="#ff6666"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    complement(495..497)
                     /locus_tag="Pam(1)"
                     /label="Pam(1)"
                     /ApEinfo_label="Pam"
                     /ApEinfo_fwdcolor="#999999"
                     /ApEinfo_revcolor="#999999"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
ORIGIN
        1 cgacgttgta aaacgacggc cagtgagcgc gcgtaatacg actcactata gggcgaattg
       61 gagctaCCCG Cattaccctg ttatccctaA GATCCgccta tttcccatga ttccttcata
      121 tttgcatata cgatacaagg ctgttagaga gataattaga attaatttga ctgtaaacac
      181 aaagatatta gtacaaaata cgtgacgtag aaagtaataa tttcttgggt agtttgcagt
      241 tttaaaatta tgttttaaaa tggactatca tatgcttacc gtaacttgaa agtatttcga
      301 tttcttggct ttatatatct tGTGGAAAGG ACGAAACACC ggtatgtcgg gaacctctcc
      361 gttttagagc taGAAAtagc aagttaaaat aaggctagtc cgttatcaac ttgaaaaagt
      421 ggcaccgagt cggtgcTTTT TTgaattcAG ATCTTACatt tcaggacatc tagcttatcg
      481 ataccgtcGA CTACcctgga gaggttcccg acatacctaC TGACCGACGA CCTGCCCAGG
      541 GCCTCGGACT ATAAGGACGA CGACGACAAG GGCGGAGGCG GAGGGGAGAA CCTGTACTTT
      601 CAGGGAAGCG GAGGAGGAGG CAAAGAAACA GCTGCTGCTA AATTCGAGCG GCAGCACATG
      661 GACAGCTCTG GCCCCTCTGG CAGCGCCGCC AAGTTCAGCA GCCTGGAAGT GCTGTTCCAG
      721 GGCCCCCTGA GCAGCAGCGG CCCCAGCGGC AGCATGGTGT CCAAGGGCGA GGAACTGTTC
      781 ACCGGCGTGG TGCCCATCCT GGTGGAGCTG GACGGCGACG TGAACGGCCA CAAGTTCAGC
      841 GTGTCCGGCG AGGGCGAAGG CGACGCCACC TACGGCAAGC TGACCCTGAA GTTCATCTGC
      901 ACCACCGGCA AGCTGCCCGT GCCCTGGCCC ACCCTGGTGA CCACCCTGAC CTACGGCGTG
      961 CAGTGCTTCA GCAGATACCC CGACCACATG AAGCAGCACG ATTTCTTCAA GAGCGCCATG
     1021 CCCGAGGGCT ACGTCCAggA GCGCACCATC TTTTTCAAGG ACGACGGCAA CTACAAGACC
     1081 AGGGCCGAGG TGAAGTTCGA GGGCGACACA CTGGTGAACA GGATCGAGCT GAAGGGCATC
     1141 GACTTCAAAG AGGACGGCAA CATCCTGGGC CACAAGCTGG AATACAACTA CAACAGCCAC
     1201 AACGTGTACA TTATGGCCGA CAAGCAGAAG AACGGCATCA AGGTCAACTT CAAGATCCGG
     1261 CACAACATCG AGGACGGCAG CGTGCAGCTG GCCGACCACT ACCAGCAGAA CACCCCCATC
     1321 GGCGACGGCC CCGTGCTGCT GCCCGACAAC CACTACCTGA GCACCCAGAG CGCTCTCAGC
     1381 AAGGACCCCA ACGAGAAGCG GGACCACATG GTGCTGAAAG AATTTGTGAC AGCCGCCGGA
     1441 ATCACCCTGG GCATGGACGA GCTGTACAAG cgCGGAGCCA CGAACTTCTC TCTGTTAAAG
     1501 CAAGCAGGAG ATGTTGAAGA AAACCCCGGG Cccatgaaaa agcctgaact caccgcgacg
     1561 tctgtcgaga agtttctgat cgaaaagttc gacagcgtct ccgacctgat gcagctctcg
     1621 gagggcgaag aatctcgtgc tttcagcttc gatgtaggag ggcgtggata tgtcctgcgg
     1681 gtaaatagct gcgccgatgg tttctacaaa gatcgttatg tttatcggca ctttgcatcg
     1741 gccgcgctcc cgattccgga agtgcttgac attggggaat tcagcgagag cctgacctat
     1801 tgcatctccc gccgtgcaca gggtgtcacg ttgcaagacc tgcctgaaac cgaactgccc
     1861 gctgttctgc agccggtcgc ggaggccatg gatgcgatcg ctgcggccga tcttagccag
     1921 acgagcgggt tcggcccatt cggaccgcaa ggaatcggtc aatacactac atggcgtgat
     1981 ttcatatgcg cgattgctga tccccatgtg tatcactggc aaactgtgat ggacgacacc
     2041 gtcagtgcgt ccgtcgcgca ggctctcgat gagctgatgc tttgggccga ggactgcccc
     2101 gaagtccggc acctcgtgca cgcggatttc ggctccaaca atgtcctgac ggacaatggc
     2161 cgcataacag cggtcattga ctggagcgag gcgatgttcg gggattccca atacgaggtc
     2221 gccaacatct tcttctggag gccgtggttg gcttgtatgg agcagcagac gcgctacttc
     2281 gagcggaggc atccggagct tgcaggatcg ccgcggctcc gggcgtatat gctccgcatt
     2341 ggtcttgacc aactctatca gagcttggtt gacggcaatt tcgatgatgc agcttgggcg
     2401 cagggtcgat gcgacgcaat cgtccgatcc ggagccggga ctgtcgggcg tacacaaatc
     2461 gcccgcagaa gcgcggccgt ctggaccgat ggctgtgtag aagtactcgc cgatagtgga
     2521 aaccgacgcc ccagcactcg tccgagggca aaggaaTGAc ccctggagag gttcccgaca
     2581 taccagatgt CTCgaggggg ggcccggtac ccagcttttg ttccctttag tgagggttaa
     2641 ttgcgcgctt ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt tatccgctca
     2701 caattccaca caacatacga gccggaagca taaagtgtaa agcctggggt gcctaatgag
     2761 tgagctaact cacattaatt gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt
     2821 cgtgccagct gcattaatga atcggccaac gcgcggggag aggcggtttg cgtattgggc
     2881 gctcttccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg
     2941 tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa
     3001 agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg
     3061 cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga
     3121 ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg
     3181 tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg
     3241 gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc
     3301 gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg
     3361 gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca
     3421 ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt
     3481 ggcctaacta cggctacact agaaggacag tatttggtat ctgcgctctg ctgaagccag
     3541 ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg
     3601 gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc
     3661 ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt
     3721 tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt
     3781 ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca
     3841 gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg
     3901 tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac
     3961 cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg
     4021 ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc
     4081 gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta
     4141 caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac
     4201 gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc
     4261 ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac
     4321 tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact
     4381 caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa
     4441 tacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt
     4501 cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca
     4561 ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa
     4621 aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac
     4681 tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg
     4741 gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc
     4801 gaaaagtgcc acctaaattg taagcgttaa tattttgtta aaattcgcgt taaatttttg
     4861 ttaaatcagc tcatttttta accaataggc cgaaatcggc aaaatccctt ataaatcaaa
     4921 agaatagacc gagatagggt tgagtgttgt tccagtttgg aacaagagtc cactattaaa
     4981 gaacgtggac tccaacgtca aagggcgaaa aaccgtctat cagggcgatg gcccactacg
     5041 tgaaccatca ccctaatcaa gttttttggg gtcgaggtgc cgtaaagcac taaatcggaa
     5101 ccctaaaggg agcccccgat ttagagcttg acggggaaag ccggcgaacg tggcgagaaa
     5161 ggaagggaag aaagcgaaag gagcgggcgc tagggcgctg gcaagtgtag cggtcacgct
     5221 gcgcgtaacc accacacccg ccgcgcttaa tgcgccgcta cagggcgcgt cccattcgcc
     5281 attcaggctg cgcaactgtt gggaagggcg atcggtgcgg gcctcttcgc tattacgcca
     5341 gctggcgaaa gggggatgtg ctgcaaggcg attaagttgg gtaacgccag ggttttccca
     5401 gtca     
//
