LOCUS       pGTag_NGFR_EGFP_        5227 bp    DNA        circular     16-NOV-2022
DEFINITION  
ACCESSION   
VERSION     
SOURCE      
  ORGANISM  
COMMENT     
COMMENT     ApEinfo:methylated:1
FEATURES             Location/Qualifiers
     primer_bind     2482..2497
                     /vntifkey="28"
                     /locus_tag="M13rev"
                     /label="M13rev"
                     /ApEinfo_label="M13rev"
                     /ApEinfo_fwdcolor="pink"
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     primer_bind     3..24
                     /vntifkey="28"
                     /locus_tag="M13\for"
                     /label="M13\for"
                     /ApEinfo_label="M13\for"
                     /ApEinfo_fwdcolor="pink"
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     polyA_signal    64..65
                     /vntifkey="25"
                     /locus_tag="bpA"
                     /label="bpA"
                     /ApEinfo_label="bpA"
                     /ApEinfo_fwdcolor="pink"
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    342..360
                     /locus_tag="CRISPR"
                     /label="CRISPR"
                     /ApEinfo_label="CRISPR"
                     /ApEinfo_fwdcolor="#66ff66"
                     /ApEinfo_revcolor="#ff6666"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     protein_bind    2240..2281
                     /locus_tag="S-pept(hum.adopt)"
                     /label="S-pept(hum.adopt)"
                     /ApEinfo_label="S-pept(hum.adopt)"
                     /ApEinfo_fwdcolor="#009a9a"
                     /ApEinfo_revcolor="#009a9a"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    337..340
                     /locus_tag="Overhang 3"
                     /label="Overhang 3"
                     /ApEinfo_label="Overhang 3"
                     /ApEinfo_fwdcolor="#ff80c0"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    1433..1717
                     /locus_tag="NGFP-exon1"
                     /label="NGFP-exon1"
                     /ApEinfo_label="NGFP-exon1"
                     /ApEinfo_fwdcolor="#2bff27"
                     /ApEinfo_revcolor="#2bff27"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    101..332
                     /locus_tag="hU6"
                     /label="hU6"
                     /ApEinfo_label="hU6"
                     /ApEinfo_fwdcolor="#0000ff"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    361..364
                     /locus_tag="Overhang 5"
                     /label="Overhang 5"
                     /ApEinfo_label="Overhang 5"
                     /ApEinfo_fwdcolor="#ff80c0"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     protein_bind    2333..2356
                     /locus_tag="FLAG(hum.adopt)"
                     /label="FLAG(hum.adopt)"
                     /ApEinfo_label="FLAG(hum.adopt)"
                     /ApEinfo_fwdcolor="#ff2834"
                     /ApEinfo_revcolor="#ff2834"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     primer_bind     complement(2445..2464)
                     /locus_tag="T3"
                     /label="T3"
                     /ApEinfo_label="T3"
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    2171..2206
                     /locus_tag="PreScission (hum.adopt)"
                     /label="PreScission (hum.adopt)"
                     /ApEinfo_label="PreScission (hum.adopt)"
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="cyan"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     primer_bind     7..24
                     /locus_tag="M13-fwd"
                     /label="M13-fwd"
                     /ApEinfo_label="M13-fwd"
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    2297..2317
                     /locus_tag="TEV(hum.adopt)"
                     /label="TEV(hum.adopt)"
                     /ApEinfo_label="TEV(hum.adopt)"
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="cyan"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     primer_bind     complement(2482..2502)
                     /locus_tag="M13-rev"
                     /label="M13-rev"
                     /ApEinfo_label="M13-rev"
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     primer_bind     33..53
                     /locus_tag="T7"
                     /label="T7"
                     /ApEinfo_label="T7"
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    1372..1430
                     /locus_tag="P2A"
                     /label="P2A"
                     /ApEinfo_label="P2A"
                     /ApEinfo_fwdcolor="#ff8000"
                     /ApEinfo_revcolor="#ff0000"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     rep_origin      complement(2870..3552)
                     /locus_tag="ColE1 origin"
                     /label="ColE1 origin"
                     /ApEinfo_label="ColE1 origin"
                     /ApEinfo_fwdcolor="gray50"
                     /ApEinfo_revcolor="gray50"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     rep_origin      complement(4770..5076)
                     /locus_tag="F1 ori"
                     /label="F1 ori"
                     /ApEinfo_label="F1 ori"
                     /ApEinfo_fwdcolor="gray50"
                     /ApEinfo_revcolor="gray50"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    72..89
                     /vntifkey="21"
                     /locus_tag="I-Sce"
                     /label="I-Sce"
                     /ApEinfo_label="I-Sce"
                     /ApEinfo_fwdcolor="#cccccc"
                     /ApEinfo_revcolor="#cccccc"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     CDS             complement(5095..5163)
                     /locus_tag="LacZ alpha"
                     /label="LacZ alpha"
                     /ApEinfo_label="LacZ alpha"
                     /ApEinfo_fwdcolor="#6495ed"
                     /ApEinfo_revcolor="#6495ed"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_binding    complement(2508..2530)
                     /locus_tag="LacO"
                     /label="LacO"
                     /ApEinfo_label="LacO"
                     /ApEinfo_fwdcolor="#6495ed"
                     /ApEinfo_revcolor="#6495ed"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     CDS             complement(3650..4309)
                     /locus_tag="AmpR"
                     /label="AmpR"
                     /ApEinfo_label="AmpR"
                     /ApEinfo_fwdcolor="yellow"
                     /ApEinfo_revcolor="yellow"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     polyA_signal    2417..2418
                     /vntifkey="25"
                     /locus_tag="bpA(1)"
                     /label="bpA(1)"
                     /ApEinfo_label="bpA"
                     /ApEinfo_fwdcolor="pink"
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    499..518
                     /locus_tag="CRISPR(1)"
                     /label="CRISPR(1)"
                     /ApEinfo_label="CRISPR"
                     /ApEinfo_fwdcolor="#66ff66"
                     /ApEinfo_revcolor="#ff6666"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    2386..2405
                     /locus_tag="CRISPR(2)"
                     /label="CRISPR(2)"
                     /ApEinfo_label="CRISPR"
                     /ApEinfo_fwdcolor="#66ff66"
                     /ApEinfo_revcolor="#ff6666"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    519..521
                     /locus_tag="Pam"
                     /label="Pam"
                     /ApEinfo_label="Pam"
                     /ApEinfo_fwdcolor="#999999"
                     /ApEinfo_revcolor="#999999"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    2406..2408
                     /locus_tag="Pam(1)"
                     /label="Pam(1)"
                     /ApEinfo_label="Pam"
                     /ApEinfo_fwdcolor="#999999"
                     /ApEinfo_revcolor="#999999"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    1718..2149
                     /locus_tag="NGFP-exon2"
                     /label="NGFP-exon2"
                     /ApEinfo_label="NGFP-exon2"
                     /ApEinfo_fwdcolor="#17ff51"
                     /ApEinfo_revcolor="#17ff51"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    530..1369
                     /note="LNGFR open reading frame"
                     /locus_tag="LNGFR"
                     /label="LNGFR"
                     /ApEinfo_label="LNGFR"
                     /ApEinfo_fwdcolor="#800080"
                     /ApEinfo_revcolor="#800080"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
ORIGIN
        1 cgacgttgta aaacgacggc cagtgagcgc gcgtaatacg actcactata gggcgaattg
       61 gagctaCCCG Cattaccctg ttatccctaA GATCCgccta tttcccatga ttccttcata
      121 tttgcatata cgatacaagg ctgttagaga gataattaga attaatttga ctgtaaacac
      181 aaagatatta gtacaaaata cgtgacgtag aaagtaataa tttcttgggt agtttgcagt
      241 tttaaaatta tgttttaaaa tggactatca tatgcttacc gtaacttgaa agtatttcga
      301 tttcttggct ttatatatct tGTGGAAAGG ACGAAACACC ggtatgtcgg gaacctctcc
      361 gttttagagc taGAAAtagc aagttaaaat aaggctagtc cgttatcaac ttgaaaaagt
      421 ggcaccgagt cggtgcTTTT TTgaattcAG ATCTTACatt tcaggacatc tagcttatcg
      481 ataccgtcGA CTACtttagg tatgtcggga acctctccag gGTACCACCa tgggggcagg
      541 tgccaccggc cgcgccatgg acgggccgcg cctgctgctg ttgctgcttc tgggggtgtc
      601 ccttggaggt gccaaggagg catgccccac aggcctgtac acacacagcg gtgagtgctg
      661 caaagcctgc aacctgggcg agggtgtggc ccagccttgt ggagccaacc agaccgtgtg
      721 tgagccctgc ctggacagcg tgacgttctc cgacgtggtg agcgcgaccg agccgtgcaa
      781 gccgtgcacc gagtgcgtgg ggctccagag catgtcggcg ccgtgcgtgg aggccgacga
      841 cgccgtgtgc cgctgcgcct acggctacta ccaggatgag acgactgggc gctgcgaggc
      901 gtgccgcgtg tgcgaggcgg gctcgggcct cgtgttctcc tgccaggaca agcagaacac
      961 cgtgtgcgag gagtgccccg acggcaccta ttccgacgag gccaaccacg tggacccgtg
     1021 cctgccctgc accgtgtgcg aggacaccga gcgccagctc cgcgagtgca cacgctgggc
     1081 cgacgccgag tgcgaggaga tccctggccg ttggattaca cggtccacac ccccagaggg
     1141 ctcggacagc acagccccca gcacccagga gcctgaggca cctccagaac aagacctcat
     1201 agccagcacg gtggcaggtg tggtgaccac agtgatgggc agctcccagc ccgtggtgac
     1261 ccgaggcacc accgacaacc tcatccctgt ctattgctcc atcctggctg ctgtggttgt
     1321 gggccttgtg gcctacatag ccttcaagag gtggaacagg gggatcctcc gCGGAGCCAC
     1381 GAACTTCTCT CTGTTAAAGC AAGCAGGAGA TGTTGAAGAA AACCCCGGGC ccATGGTGTC
     1441 CAAGGGCGAG GAACTGTTCA CCGGCGTGGT GCCCATCCTG GTGGAGCTGG ACGGCGACGT
     1501 GAACGGCCAC AAGTTCAGCG TGTCCGGCGA GGGCGAAGGC GACGCCACCT ACGGCAAGCT
     1561 GACCCTGAAG TTCATCTGCA CCACCGGCAA GCTGCCCGTG CCCTGGCCCA CCCTGGTGAC
     1621 CACCCTGACC TACGGCGTGC AGTGCTTCAG CAGATACCCC GACCACATGA AGCAGCACGA
     1681 TTTCTTCAAG AGCGCCATGC CCGAGGGCTA CGTCCAggAG CGCACCATCT TTTTCAAGGA
     1741 CGACGGCAAC TACAAGACCA GGGCCGAGGT GAAGTTCGAG GGCGACACAC TGGTGAACAG
     1801 GATCGAGCTG AAGGGCATCG ACTTCAAAGA GGACGGCAAC ATCCTGGGCC ACAAGCTGGA
     1861 ATACAACTAC AACAGCCACA ACGTGTACAT TATGGCCGAC AAGCAGAAGA ACGGCATCAA
     1921 GGTCAACTTC AAGATCCGGC ACAACATCGA GGACGGCAGC GTGCAGCTGG CCGACCACTA
     1981 CCAGCAGAAC ACCCCCATCG GCGACGGCCC CGTGCTGCTG CCCGACAACC ACTACCTGAG
     2041 CACCCAGAGC GCTCTCAGCA AGGACCCCAA CGAGAAGCGG GACCACATGG TGCTGAAAGA
     2101 ATTTGTGACA GCCGCCGGAA TCACCCTGGG CATGGACGAG CTGTACAAGA GCAGCGGCCC
     2161 CAGCGGCAGC AGCAGCCTGG AAGTGCTGTT CCAGGGCCCC CTGAGCAGCT CTGGCCCCTC
     2221 TGGCAGCGCC GCCAAGTTCA AAGAAACAGC TGCTGCTAAA TTCGAGCGGC AGCACATGGA
     2281 CAGCGGAGGA GGAGGCGAGA ACCTGTACTT TCAGGGAGGC GGAGGCGGAG GGGACTATAA
     2341 GGACGACGAC GACAAGCTGA CCGACGACCT GCCCAGGGCC TCGttggtat gtcgggaacc
     2401 tctccaggga tgtCTCgagg gggggcccgg tacccagctt ttgttccctt tagtgagggt
     2461 taattgcgcg cttggcgtaa tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc
     2521 tcacaattcc acacaacata cgagccggaa gcataaagtg taaagcctgg ggtgcctaat
     2581 gagtgagcta actcacatta attgcgttgc gctcactgcc cgctttccag tcgggaaacc
     2641 tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg
     2701 ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag
     2761 cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag
     2821 gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc
     2881 tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc
     2941 agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc
     3001 tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt
     3061 cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg
     3121 ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat
     3181 ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag
     3241 ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt
     3301 ggtggcctaa ctacggctac actagaagga cagtatttgg tatctgcgct ctgctgaagc
     3361 cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta
     3421 gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag
     3481 atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga
     3541 ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa
     3601 gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa
     3661 tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc
     3721 ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga
     3781 taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa
     3841 gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt
     3901 gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg
     3961 ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc
     4021 aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg
     4081 gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag
     4141 cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt
     4201 actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt
     4261 caatacggga taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac
     4321 gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac
     4381 ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag
     4441 caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa
     4501 tactcatact cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga
     4561 gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc
     4621 cccgaaaagt gccacctaaa ttgtaagcgt taatattttg ttaaaattcg cgttaaattt
     4681 ttgttaaatc agctcatttt ttaaccaata ggccgaaatc ggcaaaatcc cttataaatc
     4741 aaaagaatag accgagatag ggttgagtgt tgttccagtt tggaacaaga gtccactatt
     4801 aaagaacgtg gactccaacg tcaaagggcg aaaaaccgtc tatcagggcg atggcccact
     4861 acgtgaacca tcaccctaat caagtttttt ggggtcgagg tgccgtaaag cactaaatcg
     4921 gaaccctaaa gggagccccc gatttagagc ttgacgggga aagccggcga acgtggcgag
     4981 aaaggaaggg aagaaagcga aaggagcggg cgctagggcg ctggcaagtg tagcggtcac
     5041 gctgcgcgta accaccacac ccgccgcgct taatgcgccg ctacagggcg cgtcccattc
     5101 gccattcagg ctgcgcaact gttgggaagg gcgatcggtg cgggcctctt cgctattacg
     5161 ccagctggcg aaagggggat gtgctgcaag gcgattaagt tgggtaacgc cagggttttc
     5221 ccagtca     
//
