LOCUS       pTarg_bTag__2__D        5391 bp    DNA        circular     15-NOV-2022
DEFINITION  
ACCESSION   
VERSION     
SOURCE      
  ORGANISM  
COMMENT     
COMMENT     
COMMENT     ApEinfo:methylated:1
FEATURES             Location/Qualifiers
     primer_bind     2646..2661
                     /vntifkey="28"
                     /locus_tag="M13rev"
                     /label="M13rev"
                     /ApEinfo_label="M13rev"
                     /ApEinfo_fwdcolor="pink"
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    342..360
                     /locus_tag="CRISPR"
                     /label="CRISPR"
                     /ApEinfo_label="CRISPR"
                     /ApEinfo_fwdcolor="#66ff66"
                     /ApEinfo_revcolor="#ff6666"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    337..340
                     /locus_tag="Overhang 3"
                     /label="Overhang 3"
                     /ApEinfo_label="Overhang 3"
                     /ApEinfo_fwdcolor="#ff80c0"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    2570..2572
                     /locus_tag="Pam"
                     /label="Pam"
                     /ApEinfo_label="Pam"
                     /ApEinfo_fwdcolor="#999999"
                     /ApEinfo_revcolor="#999999"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    101..332
                     /locus_tag="hU6"
                     /label="hU6"
                     /ApEinfo_label="hU6"
                     /ApEinfo_fwdcolor="#0000ff"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     primer_bind     3..24
                     /vntifkey="28"
                     /locus_tag="M13\for"
                     /label="M13\for"
                     /ApEinfo_label="M13\for"
                     /ApEinfo_fwdcolor="pink"
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    2272..2315
                     /locus_tag="TagBFP"
                     /label="TagBFP"
                     /ApEinfo_label="TagBFP"
                     /ApEinfo_fwdcolor="#004080"
                     /ApEinfo_revcolor="#004080"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     protein_bind    2499..2522
                     /locus_tag="FLAG(hum.adopt)"
                     /label="FLAG(hum.adopt)"
                     /ApEinfo_label="FLAG(hum.adopt)"
                     /ApEinfo_fwdcolor="#ff2834"
                     /ApEinfo_revcolor="#ff2834"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     polyA_signal    64..65
                     /vntifkey="25"
                     /locus_tag="bpA"
                     /label="bpA"
                     /ApEinfo_label="bpA"
                     /ApEinfo_fwdcolor="pink"
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     primer_bind     complement(2609..2628)
                     /locus_tag="T3"
                     /label="T3"
                     /ApEinfo_label="T3"
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    72..89
                     /vntifkey="21"
                     /locus_tag="I-Sce"
                     /label="I-Sce"
                     /ApEinfo_label="I-Sce"
                     /ApEinfo_fwdcolor="#cccccc"
                     /ApEinfo_revcolor="#cccccc"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    531..1553
                     /locus_tag="Hygro"
                     /label="Hygro"
                     /ApEinfo_label="Hygro"
                     /ApEinfo_fwdcolor="#ff6666"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     primer_bind     complement(2646..2666)
                     /locus_tag="M13-rev"
                     /label="M13-rev"
                     /ApEinfo_label="M13-rev"
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     primer_bind     7..24
                     /locus_tag="M13-fwd"
                     /label="M13-fwd"
                     /ApEinfo_label="M13-fwd"
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     primer_bind     33..53
                     /locus_tag="T7"
                     /label="T7"
                     /ApEinfo_label="T7"
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     rep_origin      complement(3034..3716)
                     /locus_tag="ColE1 origin"
                     /label="ColE1 origin"
                     /ApEinfo_label="ColE1 origin"
                     /ApEinfo_fwdcolor="gray50"
                     /ApEinfo_revcolor="gray50"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     rep_origin      complement(4934..5240)
                     /locus_tag="F1 ori"
                     /label="F1 ori"
                     /ApEinfo_label="F1 ori"
                     /ApEinfo_fwdcolor="gray50"
                     /ApEinfo_revcolor="gray50"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     CDS             complement(5259..5327)
                     /locus_tag="LacZ alpha"
                     /label="LacZ alpha"
                     /ApEinfo_label="LacZ alpha"
                     /ApEinfo_fwdcolor="#6495ed"
                     /ApEinfo_revcolor="#6495ed"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_binding    complement(2672..2694)
                     /locus_tag="LacO"
                     /label="LacO"
                     /ApEinfo_label="LacO"
                     /ApEinfo_fwdcolor="#6495ed"
                     /ApEinfo_revcolor="#6495ed"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     CDS             complement(3814..4473)
                     /locus_tag="AmpR"
                     /label="AmpR"
                     /ApEinfo_label="AmpR"
                     /ApEinfo_fwdcolor="yellow"
                     /ApEinfo_revcolor="yellow"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     polyA_signal    2581..2582
                     /vntifkey="25"
                     /locus_tag="bpA(1)"
                     /label="bpA(1)"
                     /ApEinfo_label="bpA"
                     /ApEinfo_fwdcolor="pink"
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    498..517
                     /locus_tag="CRISPR(1)"
                     /label="CRISPR(1)"
                     /ApEinfo_label="CRISPR"
                     /ApEinfo_fwdcolor="#66ff66"
                     /ApEinfo_revcolor="#ff6666"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    361..364
                     /locus_tag="Overhang 5"
                     /label="Overhang 5"
                     /ApEinfo_label="Overhang 5"
                     /ApEinfo_fwdcolor="#ff80c0"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    518..520
                     /locus_tag="Pam(1)"
                     /label="Pam(1)"
                     /ApEinfo_label="Pam"
                     /ApEinfo_fwdcolor="#999999"
                     /ApEinfo_revcolor="#999999"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    2550..2569
                     /locus_tag="CRISPR(2)"
                     /label="CRISPR(2)"
                     /ApEinfo_label="CRISPR"
                     /ApEinfo_fwdcolor="#66ff66"
                     /ApEinfo_revcolor="#ff6666"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     protein_bind    2406..2447
                     /locus_tag="S-pept(hum.adopt)"
                     /label="S-pept(hum.adopt)"
                     /ApEinfo_label="S-pept(hum.adopt)"
                     /ApEinfo_fwdcolor="#009a9a"
                     /ApEinfo_revcolor="#009a9a"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    2337..2372
                     /locus_tag="PreScission (hum.adopt)"
                     /label="PreScission (hum.adopt)"
                     /ApEinfo_label="PreScission (hum.adopt)"
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="cyan"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    2463..2483
                     /locus_tag="TEV(hum.adopt)"
                     /label="TEV(hum.adopt)"
                     /ApEinfo_label="TEV(hum.adopt)"
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="cyan"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    1556..1614
                     /locus_tag="P2A"
                     /label="P2A"
                     /ApEinfo_label="P2A"
                     /ApEinfo_fwdcolor="#ff8000"
                     /ApEinfo_revcolor="#ff0000"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    1617..2271
                     /locus_tag="TagBFP(1)"
                     /label="TagBFP(1)"
                     /ApEinfo_label="TagBFP"
                     /ApEinfo_fwdcolor="#004080"
                     /ApEinfo_revcolor="#004080"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
ORIGIN
        1 cgacgttgta aaacgacggc cagtgagcgc gcgtaatacg actcactata gggcgaattg
       61 gagctaCCCG Cattaccctg ttatccctaA GATCCgccta tttcccatga ttccttcata
      121 tttgcatata cgatacaagg ctgttagaga gataattaga attaatttga ctgtaaacac
      181 aaagatatta gtacaaaata cgtgacgtag aaagtaataa tttcttgggt agtttgcagt
      241 tttaaaatta tgttttaaaa tggactatca tatgcttacc gtaacttgaa agtatttcga
      301 tttcttggct ttatatatct tGTGGAAAGG ACGAAACACC ggtatgtcgg gaacctctcc
      361 gttttagagc taGAAAtagc aagttaaaat aaggctagtc cgttatcaac ttgaaaaagt
      421 ggcaccgagt cggtgcTTTT TTgaattcAG ATCTTACatt tcaggacatc tagcttatcg
      481 ataccgtcGA CTACttaggt atgtcgggaa cctctccagg gaGTACCACC atgaaaaagc
      541 ctgaactcac cgcgacgtct gtcgagaagt ttctgatcga aaagttcgac agcgtctccg
      601 acctgatgca gctctcggag ggcgaagaat ctcgtgcttt cagcttcgat gtaggagggc
      661 gtggatatgt cctgcgggta aatagctgcg ccgatggttt ctacaaagat cgttatgttt
      721 atcggcactt tgcatcggcc gcgctcccga ttccggaagt gcttgacatt ggggaattca
      781 gcgagagcct gacctattgc atctcccgcc gtgcacaggg tgtcacgttg caagacctgc
      841 ctgaaaccga actgcccgct gttctgcagc cggtcgcgga ggccatggat gcgatcgctg
      901 cggccgatct tagccagacg agcgggttcg gcccattcgg accgcaagga atcggtcaat
      961 acactacatg gcgtgatttc atatgcgcga ttgctgatcc ccatgtgtat cactggcaaa
     1021 ctgtgatgga cgacaccgtc agtgcgtccg tcgcgcaggc tctcgatgag ctgatgcttt
     1081 gggccgagga ctgccccgaa gtccggcacc tcgtgcacgc ggatttcggc tccaacaatg
     1141 tcctgacgga caatggccgc ataacagcgg tcattgactg gagcgaggcg atgttcgggg
     1201 attcccaata cgaggtcgcc aacatcttct tctggaggcc gtggttggct tgtatggagc
     1261 agcagacgcg ctacttcgag cggaggcatc cggagcttgc aggatcgccg cggctccggg
     1321 cgtatatgct ccgcattggt cttgaccaac tctatcagag cttggttgac ggcaatttcg
     1381 atgatgcagc ttgggcgcag ggtcgatgcg acgcaatcgt ccgatccgga gccgggactg
     1441 tcgggcgtac acaaatcgcc cgcagaagcg cggccgtctg gaccgatggc tgtgtagaag
     1501 tactcgccga tagtggaaac cgacgcccca gcactcgtcc gagggcaaag gaacgCGGAG
     1561 CCACGAACTT CTCTCTGTTA AAGCAAGCAG GAGATGTTGA AGAAAACCCC GGGCccATGA
     1621 GCGAGCTGAT TAAGGAGAAC ATGCACATGA AGCTGTACAT GGAGGGCACC GTGGACAACC
     1681 ATCACTTCAA GTGCACATCC GAGGGCGAAG GCAAGCCCTA CGAGGGCACC CAGACCATGA
     1741 GAATCAAGGT GGTCGAGGGC GGCCCTCTCC CCTTCGCCTT CGACATCCTG GCTACTAGCT
     1801 TCCTCTACGG CAGCAAGACC TTCATCAACC ACACCCAGGG CATCCCCGAC TTCTTCAAGC
     1861 AGTCCTTCCC TGAGGGCTTC ACATGGGAGA GAGTCACCAC ATACGAAGAC GGGGGCGTGC
     1921 TGACCGCTAC CCAGGACACC AGCCTCCAGG ACGGCTGCCT CATCTACAAC GTCAAGATCA
     1981 GAGGGGTGAA CTTCACATCC AACGGCCCTG TGATGCAGAA GAAAACACTC GGCTGGGAGG
     2041 CCTTCACCGA GACGCTGTAC CCCGCTGACG GCGGCCTGGA AGGCAGAAAC GACATGGCCC
     2101 TGAAGCTCGT GGGCGGGAGC CATCTGATCG CAAACATCAA GACCACATAT AGATCCAAGA
     2161 AACCCGCTAA GAACCTCAAG ATGCCTGGCG TCTACTATGT GGACTACAGA CTGGAAAGAA
     2221 TCAAGGAGGC CAACAACGAG ACCTACGTCG AGCAGCACGA GGTGGCAGTG GCCAGATACT
     2281 GCGACCTCCC TAGCAAACTG GGGCACAAGC TTAATAGCAG CGGCCCCAGC GGCAGCAGCA
     2341 GCCTGGAAGT GCTGTTCCAG GGCCCCCTGA GCAGCTCTGG CCCCTCTGGC AGCGCCGCCA
     2401 AGTTCAAAGA AACAGCTGCT GCTAAATTCG AGCGGCAGCA CATGGACAGC GGAGGAGGAG
     2461 GCGAGAACCT GTACTTTCAG GGAGGCGGAG GCGGAGGGGA CTATAAGGAC GACGACGACA
     2521 AGCTGACCGA CGACCTGCCC AGGGCCTCGg gtatgtcggg aacctctcca gggatgtCTC
     2581 gagggggggc ccggtaccca gcttttgttc cctttagtga gggttaattg cgcgcttggc
     2641 gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa
     2701 catacgagcc ggaagcataa agtgtaaagc ctggggtgcc taatgagtga gctaactcac
     2761 attaattgcg ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt gccagctgca
     2821 ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt attgggcgct cttccgcttc
     2881 ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc
     2941 aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc
     3001 aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag
     3061 gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc
     3121 gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt
     3181 tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct
     3241 ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg
     3301 ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct
     3361 tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat
     3421 tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg
     3481 ctacactaga aggacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa
     3541 aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt
     3601 ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc
     3661 tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt
     3721 atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta
     3781 aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat
     3841 ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac
     3901 tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg
     3961 ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag
     4021 tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt
     4081 aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt
     4141 gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt
     4201 tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt
     4261 cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct
     4321 tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt
     4381 ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac gggataatac
     4441 cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa
     4501 actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa
     4561 ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca
     4621 aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct
     4681 ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga
     4741 atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc
     4801 taaattgtaa gcgttaatat tttgttaaaa ttcgcgttaa atttttgtta aatcagctca
     4861 ttttttaacc aataggccga aatcggcaaa atcccttata aatcaaaaga atagaccgag
     4921 atagggttga gtgttgttcc agtttggaac aagagtccac tattaaagaa cgtggactcc
     4981 aacgtcaaag ggcgaaaaac cgtctatcag ggcgatggcc cactacgtga accatcaccc
     5041 taatcaagtt ttttggggtc gaggtgccgt aaagcactaa atcggaaccc taaagggagc
     5101 ccccgattta gagcttgacg gggaaagccg gcgaacgtgg cgagaaagga agggaagaaa
     5161 gcgaaaggag cgggcgctag ggcgctggca agtgtagcgg tcacgctgcg cgtaaccacc
     5221 acacccgccg cgcttaatgc gccgctacag ggcgcgtccc attcgccatt caggctgcgc
     5281 aactgttggg aagggcgatc ggtgcgggcc tcttcgctat tacgccagct ggcgaaaggg
     5341 ggatgtgctg caaggcgatt aagttgggta acgccagggt tttcccagtc a
//
