LOCUS       pTarg_bTag__2__D        5394 bp    DNA        circular     16-NOV-2022
DEFINITION  
ACCESSION   
VERSION     
SOURCE      
  ORGANISM  
COMMENT       
COMMENT     ApEinfo:methylated:1
FEATURES             Location/Qualifiers
     primer_bind     2649..2664
                     /vntifkey="28"
                     /locus_tag="M13rev"
                     /label="M13rev"
                     /ApEinfo_label="M13rev"
                     /ApEinfo_fwdcolor="pink"
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    1617..2271
                     /locus_tag="TagBFP"
                     /label="TagBFP"
                     /ApEinfo_label="TagBFP"
                     /ApEinfo_fwdcolor="#004080"
                     /ApEinfo_revcolor="#004080"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    complement(2555..2574)
                     /locus_tag="CRISPR"
                     /label="CRISPR"
                     /ApEinfo_label="CRISPR"
                     /ApEinfo_fwdcolor="#66ff66"
                     /ApEinfo_revcolor="#ff6666"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    337..340
                     /locus_tag="Overhang 3"
                     /label="Overhang 3"
                     /ApEinfo_label="Overhang 3"
                     /ApEinfo_fwdcolor="#ff80c0"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    complement(500..519)
                     /locus_tag="CRISPR(1)"
                     /label="CRISPR(1)"
                     /ApEinfo_label="CRISPR"
                     /ApEinfo_fwdcolor="#66ff66"
                     /ApEinfo_revcolor="#ff6666"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     protein_bind    2406..2447
                     /locus_tag="S-pept(hum.adopt)"
                     /label="S-pept(hum.adopt)"
                     /ApEinfo_label="S-pept(hum.adopt)"
                     /ApEinfo_fwdcolor="#009a9a"
                     /ApEinfo_revcolor="#009a9a"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     primer_bind     complement(2612..2631)
                     /locus_tag="T3"
                     /label="T3"
                     /ApEinfo_label="T3"
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    complement(2552..2554)
                     /locus_tag="Pam"
                     /label="Pam"
                     /ApEinfo_label="Pam"
                     /ApEinfo_fwdcolor="#999999"
                     /ApEinfo_revcolor="#999999"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     protein_bind    2499..2522
                     /locus_tag="FLAG(hum.adopt)"
                     /label="FLAG(hum.adopt)"
                     /ApEinfo_label="FLAG(hum.adopt)"
                     /ApEinfo_fwdcolor="#ff2834"
                     /ApEinfo_revcolor="#ff2834"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     primer_bind     3..24
                     /vntifkey="28"
                     /locus_tag="M13\for"
                     /label="M13\for"
                     /ApEinfo_label="M13\for"
                     /ApEinfo_fwdcolor="pink"
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    2272..2315
                     /locus_tag="TagBFP(1)"
                     /label="TagBFP(1)"
                     /ApEinfo_label="TagBFP"
                     /ApEinfo_fwdcolor="#004080"
                     /ApEinfo_revcolor="#004080"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     polyA_signal    64..65
                     /vntifkey="25"
                     /locus_tag="bpA"
                     /label="bpA"
                     /ApEinfo_label="bpA"
                     /ApEinfo_fwdcolor="pink"
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     primer_bind     complement(2649..2669)
                     /locus_tag="M13-rev"
                     /label="M13-rev"
                     /ApEinfo_label="M13-rev"
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    2337..2372
                     /locus_tag="PreScission (hum.adopt)"
                     /label="PreScission (hum.adopt)"
                     /ApEinfo_label="PreScission (hum.adopt)"
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="cyan"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    531..1553
                     /locus_tag="Hygro"
                     /label="Hygro"
                     /ApEinfo_label="Hygro"
                     /ApEinfo_fwdcolor="#ff6666"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    2463..2483
                     /locus_tag="TEV(hum.adopt)"
                     /label="TEV(hum.adopt)"
                     /ApEinfo_label="TEV(hum.adopt)"
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="cyan"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    72..89
                     /vntifkey="21"
                     /locus_tag="I-Sce"
                     /label="I-Sce"
                     /ApEinfo_label="I-Sce"
                     /ApEinfo_fwdcolor="#cccccc"
                     /ApEinfo_revcolor="#cccccc"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    1556..1614
                     /locus_tag="P2A"
                     /label="P2A"
                     /ApEinfo_label="P2A"
                     /ApEinfo_fwdcolor="#ff8000"
                     /ApEinfo_revcolor="#ff0000"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     rep_origin      complement(3037..3719)
                     /locus_tag="ColE1 origin"
                     /label="ColE1 origin"
                     /ApEinfo_label="ColE1 origin"
                     /ApEinfo_fwdcolor="gray50"
                     /ApEinfo_revcolor="gray50"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     rep_origin      complement(4937..5243)
                     /locus_tag="F1 ori"
                     /label="F1 ori"
                     /ApEinfo_label="F1 ori"
                     /ApEinfo_fwdcolor="gray50"
                     /ApEinfo_revcolor="gray50"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     primer_bind     7..24
                     /locus_tag="M13-fwd"
                     /label="M13-fwd"
                     /ApEinfo_label="M13-fwd"
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     CDS             complement(5262..5330)
                     /locus_tag="LacZ alpha"
                     /label="LacZ alpha"
                     /ApEinfo_label="LacZ alpha"
                     /ApEinfo_fwdcolor="#6495ed"
                     /ApEinfo_revcolor="#6495ed"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     primer_bind     33..53
                     /locus_tag="T7"
                     /label="T7"
                     /ApEinfo_label="T7"
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_binding    complement(2675..2697)
                     /locus_tag="LacO"
                     /label="LacO"
                     /ApEinfo_label="LacO"
                     /ApEinfo_fwdcolor="#6495ed"
                     /ApEinfo_revcolor="#6495ed"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     CDS             complement(3817..4476)
                     /locus_tag="AmpR"
                     /label="AmpR"
                     /ApEinfo_label="AmpR"
                     /ApEinfo_fwdcolor="yellow"
                     /ApEinfo_revcolor="yellow"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     polyA_signal    2584..2585
                     /vntifkey="25"
                     /locus_tag="bpA(1)"
                     /label="bpA(1)"
                     /ApEinfo_label="bpA"
                     /ApEinfo_fwdcolor="pink"
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    361..364
                     /locus_tag="Overhang 5"
                     /label="Overhang 5"
                     /ApEinfo_label="Overhang 5"
                     /ApEinfo_fwdcolor="#ff80c0"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    342..360
                     /locus_tag="CRISPR(2)"
                     /label="CRISPR(2)"
                     /ApEinfo_label="CRISPR"
                     /ApEinfo_fwdcolor="#66ff66"
                     /ApEinfo_revcolor="#ff6666"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    101..332
                     /locus_tag="hU6"
                     /label="hU6"
                     /ApEinfo_label="hU6"
                     /ApEinfo_fwdcolor="#0000ff"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    complement(497..499)
                     /locus_tag="Pam(1)"
                     /label="Pam(1)"
                     /ApEinfo_label="Pam"
                     /ApEinfo_fwdcolor="#999999"
                     /ApEinfo_revcolor="#999999"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
ORIGIN
        1 cgacgttgta aaacgacggc cagtgagcgc gcgtaatacg actcactata gggcgaattg
       61 gagctaCCCG Cattaccctg ttatccctaA GATCCgccta tttcccatga ttccttcata
      121 tttgcatata cgatacaagg ctgttagaga gataattaga attaatttga ctgtaaacac
      181 aaagatatta gtacaaaata cgtgacgtag aaagtaataa tttcttgggt agtttgcagt
      241 tttaaaatta tgttttaaaa tggactatca tatgcttacc gtaacttgaa agtatttcga
      301 tttcttggct ttatatatct tGTGGAAAGG ACGAAACACC ggtatgtcgg gaacctctcc
      361 gttttagagc taGAAAtagc aagttaaaat aaggctagtc cgttatcaac ttgaaaaagt
      421 ggcaccgagt cggtgcTTTT TTgaattcAG ATCTTACatt tcaggacatc tagcttatcg
      481 ataccgtcat CTACTCcctg gagaggttcc cgacatacct aaGTACCACC atgaaaaagc
      541 ctgaactcac cgcgacgtct gtcgagaagt ttctgatcga aaagttcgac agcgtctccg
      601 acctgatgca gctctcggag ggcgaagaat ctcgtgcttt cagcttcgat gtaggagggc
      661 gtggatatgt cctgcgggta aatagctgcg ccgatggttt ctacaaagat cgttatgttt
      721 atcggcactt tgcatcggcc gcgctcccga ttccggaagt gcttgacatt ggggaattca
      781 gcgagagcct gacctattgc atctcccgcc gtgcacaggg tgtcacgttg caagacctgc
      841 ctgaaaccga actgcccgct gttctgcagc cggtcgcgga ggccatggat gcgatcgctg
      901 cggccgatct tagccagacg agcgggttcg gcccattcgg accgcaagga atcggtcaat
      961 acactacatg gcgtgatttc atatgcgcga ttgctgatcc ccatgtgtat cactggcaaa
     1021 ctgtgatgga cgacaccgtc agtgcgtccg tcgcgcaggc tctcgatgag ctgatgcttt
     1081 gggccgagga ctgccccgaa gtccggcacc tcgtgcacgc ggatttcggc tccaacaatg
     1141 tcctgacgga caatggccgc ataacagcgg tcattgactg gagcgaggcg atgttcgggg
     1201 attcccaata cgaggtcgcc aacatcttct tctggaggcc gtggttggct tgtatggagc
     1261 agcagacgcg ctacttcgag cggaggcatc cggagcttgc aggatcgccg cggctccggg
     1321 cgtatatgct ccgcattggt cttgaccaac tctatcagag cttggttgac ggcaatttcg
     1381 atgatgcagc ttgggcgcag ggtcgatgcg acgcaatcgt ccgatccgga gccgggactg
     1441 tcgggcgtac acaaatcgcc cgcagaagcg cggccgtctg gaccgatggc tgtgtagaag
     1501 tactcgccga tagtggaaac cgacgcccca gcactcgtcc gagggcaaag gaacgCGGAG
     1561 CCACGAACTT CTCTCTGTTA AAGCAAGCAG GAGATGTTGA AGAAAACCCC GGGCccATGA
     1621 GCGAGCTGAT TAAGGAGAAC ATGCACATGA AGCTGTACAT GGAGGGCACC GTGGACAACC
     1681 ATCACTTCAA GTGCACATCC GAGGGCGAAG GCAAGCCCTA CGAGGGCACC CAGACCATGA
     1741 GAATCAAGGT GGTCGAGGGC GGCCCTCTCC CCTTCGCCTT CGACATCCTG GCTACTAGCT
     1801 TCCTCTACGG CAGCAAGACC TTCATCAACC ACACCCAGGG CATCCCCGAC TTCTTCAAGC
     1861 AGTCCTTCCC TGAGGGCTTC ACATGGGAGA GAGTCACCAC ATACGAAGAC GGGGGCGTGC
     1921 TGACCGCTAC CCAGGACACC AGCCTCCAGG ACGGCTGCCT CATCTACAAC GTCAAGATCA
     1981 GAGGGGTGAA CTTCACATCC AACGGCCCTG TGATGCAGAA GAAAACACTC GGCTGGGAGG
     2041 CCTTCACCGA GACGCTGTAC CCCGCTGACG GCGGCCTGGA AGGCAGAAAC GACATGGCCC
     2101 TGAAGCTCGT GGGCGGGAGC CATCTGATCG CAAACATCAA GACCACATAT AGATCCAAGA
     2161 AACCCGCTAA GAACCTCAAG ATGCCTGGCG TCTACTATGT GGACTACAGA CTGGAAAGAA
     2221 TCAAGGAGGC CAACAACGAG ACCTACGTCG AGCAGCACGA GGTGGCAGTG GCCAGATACT
     2281 GCGACCTCCC TAGCAAACTG GGGCACAAGC TTAATAGCAG CGGCCCCAGC GGCAGCAGCA
     2341 GCCTGGAAGT GCTGTTCCAG GGCCCCCTGA GCAGCTCTGG CCCCTCTGGC AGCGCCGCCA
     2401 AGTTCAAAGA AACAGCTGCT GCTAAATTCG AGCGGCAGCA CATGGACAGC GGAGGAGGAG
     2461 GCGAGAACCT GTACTTTCAG GGAGGCGGAG GCGGAGGGGA CTATAAGGAC GACGACGACA
     2521 AGCTGACCGA CGACCTGCCC AGGGCCTCGt tcctggagag gttcccgaca tacctgatgt
     2581 CTCgaggggg ggcccggtac ccagcttttg ttccctttag tgagggttaa ttgcgcgctt
     2641 ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt tatccgctca caattccaca
     2701 caacatacga gccggaagca taaagtgtaa agcctggggt gcctaatgag tgagctaact
     2761 cacattaatt gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt cgtgccagct
     2821 gcattaatga atcggccaac gcgcggggag aggcggtttg cgtattgggc gctcttccgc
     2881 ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca
     2941 ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg
     3001 agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca
     3061 taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa
     3121 cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc
     3181 tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc
     3241 gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct
     3301 gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg
     3361 tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag
     3421 gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta
     3481 cggctacact agaaggacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg
     3541 aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt
     3601 tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt
     3661 ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag
     3721 attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat
     3781 ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc
     3841 tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat
     3901 aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc
     3961 acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag
     4021 aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag
     4081 agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt
     4141 ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg
     4201 agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt
     4261 tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc
     4321 tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc
     4381 attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa
     4441 taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg
     4501 aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc
     4561 caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag
     4621 gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt
     4681 cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt
     4741 tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc
     4801 acctaaattg taagcgttaa tattttgtta aaattcgcgt taaatttttg ttaaatcagc
     4861 tcatttttta accaataggc cgaaatcggc aaaatccctt ataaatcaaa agaatagacc
     4921 gagatagggt tgagtgttgt tccagtttgg aacaagagtc cactattaaa gaacgtggac
     4981 tccaacgtca aagggcgaaa aaccgtctat cagggcgatg gcccactacg tgaaccatca
     5041 ccctaatcaa gttttttggg gtcgaggtgc cgtaaagcac taaatcggaa ccctaaaggg
     5101 agcccccgat ttagagcttg acggggaaag ccggcgaacg tggcgagaaa ggaagggaag
     5161 aaagcgaaag gagcgggcgc tagggcgctg gcaagtgtag cggtcacgct gcgcgtaacc
     5221 accacacccg ccgcgcttaa tgcgccgcta cagggcgcgt cccattcgcc attcaggctg
     5281 cgcaactgtt gggaagggcg atcggtgcgg gcctcttcgc tattacgcca gctggcgaaa
     5341 gggggatgtg ctgcaaggcg attaagttgg gtaacgccag ggttttccca gtca
//
