LOCUS       Exported                8161 bp ds-DNA     circular SYN 11-NOV-2022
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8161)
  AUTHORS   Michael Wehr
  TITLE     Direct Submission
  JOURNAL   Exported Monday, Nov 14, 2022 from SnapGene 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..8161
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     promoter        209..863
                     /label=CMV promotor
                     /note="CMV promotor"
     enhancer        235..614
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     misc_feature    440
                     /label=C->G
     promoter        615..818
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     863..882
                     /label=T7 promoter/priming site
                     /note="T7 promoter/priming site"
     promoter        863..881
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     misc_feature    895..906
                     /gene="miscellaneous"
                     /label=reading Frame B
                     /note="reading Frame B"
                     /note="/SECDrawas=Region"
     protein_bind    899..923
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             929..2170
                     /codon_start=1
                     /gene="hDRD2-var2-Cop"
                     /label=hDRD2-var2-Cop
                     /note="accession: "
                     /note="/SECDrawas=Gene"
                     /note="/organism=Homo Sapiens"
                     /translation="MDPLNLSWYDDDLERQNWSRPFNGSDGKADRPHYNYYATLLTLLI
                     AVIVFGNVLVCMAVSREKALQTTTNYLIVSLAVADLLVATLVMPWVVYLEVVGEWKFSR
                     IHCDIFVTLDVMMCTASILNLCAISIDRYTAVAMPMLYNTRYSSKRRVTVMISIVWVLS
                     FTISCPLLFGLNNADQNECIIANPAFVVYSSIVSFYVPFIVTLLVYIKIYIVLRRRRKR
                     VNTKRSSRAFRAHLRAPLKEAARRAQELEMEMLSSTSPPERTRYSPIPPSHHQLTLPDP
                     SHHGLHSTPDSPAKPEKNGHAKDHPKIAKIFEIQTMPNGKTRTSLKTMSRRKLSQQKEK
                     KATQMLAIVLGVFIICWLPFFITHILNIHCDCNIPPVLYSAFTWLGYVNSAVNPIIYTT
                     FNIEFRKAFLKILHC"
     protein_bind    complement(2172..2196)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    2182..2200
                     /gene="miscellaneous"
                     /label=reading Frame B
                     /note="reading Frame B"
                     /note="/SECDrawas=Region"
     CDS             2207..2293
                     /codon_start=1
                     /gene="coding sequence"
                     /label=V2R aa343-371
                     /note="V2R aa343-371"
                     /note="/SECDrawas=Region"
                     /translation="ARGRTPPSLGPQDESCTTASSSLAKDTSS"
     CDS             2300..2329
                     /codon_start=1
                     /label=GS linker
                     /note="GS linker"
                     /translation="GGGGSGGGGS"
     CDS             2330..2683
                     /codon_start=1
                     /label=TEV1-118
                     /note="TEV1-118"
                     /translation="GESLFKGPRDYNPISSTICHLTNESDGHTTSLYGIGFGPFIITNK
                     HLFRRNNGTLLVQSLHGVFKVKDTTTLQQHLIDGRDMMIIRMPKDFPPFPQKLKFREPQ
                     REERICLVTTNFQA"
     misc_binding    complement(2563..2585)
                     /label=NTEV 5' sequencing primer
                     /note="NTEV 5' sequencing primer"
     CDS             2687..2707
                     /codon_start=1
                     /label=tevS
                     /note="tevS (TEV cleavage site)"
                     /translation="ENLYFQG"
     CDS             2687..2707
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             2714..2743
                     /codon_start=1
                     /label=Myc-tag
                     /note="Myc-tag (due to cloning reasons - taken from old 
                     pDEST_X-ASPlink-NTEV-tevS-GV which contained that Myc-tag 
                     already)"
                     /translation="EQKLISEEDL"
     CDS             2714..2743
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             2744..3640
                     /codon_start=1
                     /label=Gal4-VP16
                     /note="Gal4-VP16"
                     /translation="ARAMKLLSSIEQACDICRLKKLKCSKEKPKCAKCLKNNWECRYSP
                     KTKRSPLTRAHLTEVESRLERLEQLFLLIFPREDLDMILKMDSLQDIKALLTGLFVQDN
                     VNKDAVTDRLASVETDMPLTLRQHRISATSSSEESSNKGQRQLTVSPEFPGISLIIRFT
                     MPKKKRKVAYSRARTKNNYGSTIEGLLDLPDDDAPEEAGLAAPRLSFLPAGHTRRLSTA
                     PPTDVSLGDELHLDGEDVAMAHADALDDFDLDMLGDGDSPGPGFTPHDSAPYGALDMAD
                     FEFEQMFTDALGIDEYGG"
     misc_structure  2744..3196
                     /label=Gal4-DBD core ORF
                     /note="Gal4-DBD core ORF"
     CDS             2753..3193
                     /codon_start=1
                     /gene="Saccharomyces cerevisiae GAL4 (truncated)"
                     /product="DNA binding domain of the GAL4 transcriptional 
                     activator"
                     /label=GAL4 DNA binding domain
                     /translation="MKLLSSIEQACDICRLKKLKCSKEKPKCAKCLKNNWECRYSPKTK
                     RSPLTRAHLTEVESRLERLEQLFLLIFPREDLDMILKMDSLQDIKALLTGLFVQDNVNK
                     DAVTDRLASVETDMPLTLRQHRISATSSSEESSNKGQRQLTVS"
     misc_structure  3236..3640
                     /label=NLS-VP16
                     /note="NLS-VP16"
     CDS             3236..3256
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             3407..3640
                     /codon_start=1
                     /gene="UL48"
                     /product="transcriptional activation domain of herpes 
                     simplex virus protein VP16 (Triezenberg et al., 1988; 
                     Cousens et al., 1989)"
                     /label=VP16 AD
                     /translation="APPTDVSLGDELHLDGEDVAMAHADALDDFDLDMLGDGDSPGPGF
                     TPHDSAPYGALDMADFEFEQMFTDALGIDEYGG"
     CDS             3641..3700
                     /codon_start=1
                     /label=2xHA-tag
                     /note="2xHA-tag"
                     /translation="GYPYDVPDYAGYPYDVPDYA"
     CDS             3644..3670
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             3674..3700
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     promoter        complement(3713..3731)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     polyA_site      3733..3964
                     /label=BGH polyA
                     /note="BGH polyA"
     primer_bind     3751..3768
                     /label=BGH Reverse priming site
                     /note="BGH Reverse priming site"
     polyA_signal    3757..3981
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      4027..4455
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     promoter        4469..4799
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      4650..4785
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             4866..5660
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     polyA_signal    5715..6087
                     /label=SV40 poly A
                     /note="SV40 poly A"
     polyA_signal    5834..5955
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6004..6020)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    6028..6044
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(6052..6082)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6097..6118
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     rep_origin      complement(6347..7020)
                     /direction=LEFT
                     /label=ColE1 origin (pBR3222 origin)
                     /note="ColE1 origin (pBR3222 origin)"
     rep_origin      complement(6406..6994)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(7165..8025)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(8026..8130)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gcttatcaac
      901 aagtttgtac aaaaaagcag gctctaccat ggatccactg aatctgtcct ggtatgatga
      961 tgatctggag aggcagaact ggagccggcc cttcaacggg tcagacggga aggcggacag
     1021 accccactac aactactatg ccacactgct caccctgctc atcgctgtca tcgtcttcgg
     1081 caacgtgctg gtgtgcatgg ctgtgtctag agagaaggcg ctgcagacca ccaccaacta
     1141 cctgatcgtc agcctcgcag tggccgacct cctcgtcgcc acactggtca tgccctgggt
     1201 tgtctacctg gaggtggtag gtgagtggaa attcagcagg attcactgtg acatcttcgt
     1261 cactctggac gtcatgatgt gcacggcgag catcctgaac ttgtgtgcca tcagcatcga
     1321 caggtacaca gctgtggcca tgcccatgct gtacaatacg cgctacagct ccaagcgccg
     1381 ggtcaccgtc atgatctcca tcgtctgggt cctgtccttc accatctcct gcccactcct
     1441 cttcggactc aataacgcag accagaacga gtgcatcatt gccaacccgg ccttcgtggt
     1501 ctactcctcc atcgtctcct tctacgtgcc cttcattgtc accctgctgg tctacatcaa
     1561 gatctacatt gtcctccgca gacgccgcaa gcgagtcaac accaaacgca gcagccgagc
     1621 tttcagggcc cacctgaggg ctccactaaa ggaggctgcc cggcgagccc aggagctgga
     1681 gatggagatg ctctccagca ccagcccacc cgagaggacc cggtacagcc ccatcccacc
     1741 cagccaccac cagctgactc tccccgaccc gtcccaccac ggtctccaca gcactcctga
     1801 cagccccgcc aaaccagaga agaatgggca tgccaaagac caccccaaga ttgccaagat
     1861 ctttgagatc cagaccatgc ccaatggcaa aacccggacc tccctcaaga ccatgagccg
     1921 taggaagctc tcccagcaga aggagaagaa agccactcag atgctcgcca ttgttctcgg
     1981 cgtgttcatc atctgctggc tgcccttctt catcacacac atcctgaaca tacactgtga
     2041 ctgcaacatc ccgcctgtcc tgtacagcgc cttcacgtgg ctgggctatg tcaacagcgc
     2101 cgtgaacccc atcatctaca ccaccttcaa cattgagttc cgcaaggcct tcctgaagat
     2161 cctccactgc gacccagctt tcttgtacaa agtggttgat ggtaccgccc ggggacgcac
     2221 cccacccagc ctgggtcccc aagatgagtc ctgcaccacc gccagctcct ccctggccaa
     2281 ggacacttca tcggatgtcg gaggtggagg tagcggaggt ggaggtagcg gagagagctt
     2341 gtttaaggga ccacgtgatt acaacccgat atcgagcacc atttgtcatt tgacgaatga
     2401 atctgatggg cacacaacat cgttgtatgg tattggattt ggtcccttca tcattacaaa
     2461 caagcacttg tttcgtcgca ataatggaac actgttggtc caatcactac atggtgtatt
     2521 caaggtcaag gacaccacga ctttgcaaca acacctcatt gatgggaggg acatgatgat
     2581 tattcgcatg cctaaggatt tcccaccatt tcctcaaaag ctgaaattta gagagccaca
     2641 aagggaagag cgcatatgtc ttgtgacaac caacttccaa gctcccgaga acctgtactt
     2701 ccagggttct agagaacaga agctgatctc agaagaggat ctggcccggg ccatgaagct
     2761 actgtcttct atcgaacaag catgcgatat ttgccgactt aaaaagctca agtgctccaa
     2821 agaaaaaccg aagtgcgcca agtgtctgaa gaacaactgg gagtgtcgct actctcccaa
     2881 aaccaaaagg tctccgctga ctagggcaca tctgacagaa gtggaatcaa ggctagaaag
     2941 actggaacag ctatttctac tgatttttcc tcgagaagac cttgacatga ttttgaaaat
     3001 ggattcttta caggatataa aagcattgtt aacaggatta tttgtacaag ataatgtgaa
     3061 taaagatgcc gtcacagata gattggcttc agtggagact gatatgcctc taacattgag
     3121 acagcataga ataagtgcga catcatcatc ggaagagagt agtaacaaag gtcaaagaca
     3181 gttgactgta tcgccggaat tcccggggat cagcttgata attcgattca ccatgcccaa
     3241 gaagaagagg aaggtggcgt acagccgcgc gcgtacgaaa aacaattacg ggtctaccat
     3301 cgagggcctg ctcgatctcc cggacgacga cgcccccgaa gaggcggggc tggcggctcc
     3361 gcgcctgtcc tttctccccg cgggacacac gcgcagactg tcgacggccc ccccgaccga
     3421 tgtcagcctg ggggacgagc tccacttaga cggcgaggac gtcgcgatgg cgcatgccga
     3481 cgcgctagac gatttcgatc tggacatgtt gggggacggg gattccccgg gtccgggatt
     3541 taccccccac gactccgccc cctacggcgc tctggatatg gccgacttcg agtttgagca
     3601 gatgtttacc gatgcccttg gaattgacga gtacggtggg ggctacccat acgatgttcc
     3661 tgactatgcg ggctatccct atgacgtccc ggactatgca tgagggccct attctatagt
     3721 gtcacctaaa tgctagagct cgctgatcag cctcgactgt gccttctagt tgccagccat
     3781 ctgttgtttg cccctccccc gtgccttcct tgaccctgga aggtgccact cccactgtcc
     3841 tttcctaata aaatgaggaa attgcatcgc attgtctgag taggtgtcat tctattctgg
     3901 ggggtggggt ggggcaggac agcaaggggg aggattggga agacaatagc aggcatgctg
     3961 gggatgcggt gggctctatg gcttctgagg cggaaagaac cagctggggc tctagggggt
     4021 atccccacgc gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg
     4081 tgaccgctac acttgccagc gccctagcgc ccgctccttt cgctttcttc ccttcctttc
     4141 tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg gggcatccct ttagggttcc
     4201 gatttagtgc tttacggcac ctcgacccca aaaaacttga ttagggtgat ggttcacgta
     4261 gtgggccatc gccctgatag acggtttttc gccctttgac gttggagtcc acgttcttta
     4321 atagtggact cttgttccaa actggaacaa cactcaaccc tatctcggtc tattcttttg
     4381 atttataagg gattttgggg atttcggcct attggttaaa aaatgagctg atttaacaaa
     4441 aatttaacgc gaattaattc tgtggaatgt gtgtcagtta gggtgtggaa agtccccagg
     4501 ctccccaggc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accaggtgtg
     4561 gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag
     4621 caaccatagt cccgccccta actccgccca tcccgcccct aactccgccc agttccgccc
     4681 attctccgcc ccatggctga ctaatttttt ttatttatgc agaggccgag gccgcctctg
     4741 cctctgagct attccagaag tagtgaggag gcttttttgg aggcctaggc ttttgcaaaa
     4801 agctcccggg agcttgtata tccattttcg gatctgatca agagacagga tgaggatcgt
     4861 ttcgcatgat tgaacaagat ggattgcacg caggttctcc ggccgcttgg gtggagaggc
     4921 tattcggcta tgactgggca caacagacaa tcggctgctc tgatgccgcc gtgttccggc
     4981 tgtcagcgca ggggcgcccg gttctttttg tcaagaccga cctgtccggt gccctgaatg
     5041 aactgcagga cgaggcagcg cggctatcgt ggctggccac gacgggcgtt ccttgcgcag
     5101 ctgtgctcga cgttgtcact gaagcgggaa gggactggct gctattgggc gaagtgccgg
     5161 ggcaggatct cctgtcatct caccttgctc ctgccgagaa agtatccatc atggctgatg
     5221 caatgcggcg gctgcatacg cttgatccgg ctacctgccc attcgaccac caagcgaaac
     5281 atcgcatcga gcgagcacgt actcggatgg aagccggtct tgtcgatcag gatgatctgg
     5341 acgaagagca tcaggggctc gcgccagccg aactgttcgc caggctcaag gcgcgcatgc
     5401 ccgacggcga ggatctcgtc gtgacccatg gcgatgcctg cttgccgaat atcatggtgg
     5461 aaaatggccg cttttctgga ttcatcgact gtggccggct gggtgtggcg gaccgctatc
     5521 aggacatagc gttggctacc cgtgatattg ctgaagagct tggcggcgaa tgggctgacc
     5581 gcttcctcgt gctttacggt atcgccgctc ccgattcgca gcgcatcgcc ttctatcgcc
     5641 ttcttgacga gttcttctga gcgggactct ggggttcgaa atgaccgacc aagcgacgcc
     5701 caacctgcca tcacgagatt tcgattccac cgccgccttc tatgaaaggt tgggcttcgg
     5761 aatcgttttc cgggacgccg gctggatgat cctccagcgc ggggatctca tgctggagtt
     5821 cttcgcccac cccaacttgt ttattgcagc ttataatggt tacaaataaa gcaatagcat
     5881 cacaaatttc acaaataaag catttttttc actgcattct agttgtggtt tgtccaaact
     5941 catcaatgta tcttatcatg tctgtatacc gtcgacctct agctagagct tggcgtaatc
     6001 atggtcatag ctgtttcctg tgtgaaattg ttatccgctc acaattccac acaacatacg
     6061 agccggaagc ataaagtgta aagcctgggg tgcctaatga gtgagctaac tcacattaat
     6121 tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg tcgtgccagc tgcattaatg
     6181 aatcggccaa cgcgcgggga gaggcggttt gcgtattggg cgctcttccg cttcctcgct
     6241 cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc
     6301 ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg
     6361 ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg
     6421 cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg
     6481 actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac
     6541 cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca
     6601 atgctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt
     6661 gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc
     6721 caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag
     6781 agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac
     6841 tagaaggaca gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt
     6901 tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa
     6961 gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg
     7021 gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa
     7081 aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat
     7141 atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc
     7201 gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat
     7261 acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc
     7321 ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc
     7381 tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag
     7441 ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg tggtgtcacg
     7501 ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg
     7561 atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag
     7621 taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt
     7681 catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga
     7741 atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc
     7801 acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc
     7861 aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc
     7921 ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc
     7981 cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca
     8041 atattattga agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat
     8101 ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctgacgt
     8161 c
//
