LOCUS       Exported                8284 bp ds-DNA     circular SYN 11-NOV-2022
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8284)
  AUTHORS   Michael Wehr
  TITLE     Direct Submission
  JOURNAL   Exported Monday, Nov 14, 2022 from SnapGene 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..8284
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     enhancer        235..614
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        615..818
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     863..882
                     /label=T7 promoter/priming site
                     /note="T7 promoter/priming site"
     promoter        863..881
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     misc_feature    916..927
                     /label=rfB
                     /note="rfB"
     protein_bind    920..944
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    945..1030
                     /label=TANGO Presto 5' Signal peptide
     CDS             950..997
                     /codon_start=1
                     /label=HA1 signal peptide
                     /note="Hemagglutinin HA1 chain signal peptideInfluenza A 
                     virus
                     "
                     /translation="MKTIIALSYIFCLVFA"
     CDS             998..1021
                     /codon_start=1
                     /label=FLAG_K->A
                     /note="C-terminal K -> A
                     FLAG-tag: DYKDDDDK
                     "
                     /translation="DYKDDDDA"
     misc_feature    1034..2389
                     /label=hHTR2A
                     /note="hHTR2A"
     protein_bind    complement(2391..2415)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    2401..2419
                     /label=rfB
                     /note="rfB"
     CDS             2423..2452
                     /codon_start=1
                     /label=GS linker
                     /note="GS linker"
                     /translation="GGGGSGGGGS"
     CDS             2423..2452
                     /codon_start=1
                     /label=GS linker
                     /note="GS linker"
                     /translation="GGGGSGGGGS"
     CDS             2453..2806
                     /codon_start=1
                     /label=TEV1-118
                     /note="TEV1-118"
                     /translation="GESLFKGPRDYNPISSTICHLTNESDGHTTSLYGIGFGPFIITNK
                     HLFRRNNGTLLVQSLHGVFKVKDTTTLQQHLIDGRDMMIIRMPKDFPPFPQKLKFREPQ
                     REERICLVTTNFQA"
     misc_binding    complement(2686..2708)
                     /label=NTEV 5' sequencing primer
                     /note="NTEV 5' sequencing primer"
     CDS             2810..2830
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             2837..2866
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             2867..3763
                     /codon_start=1
                     /label=Gal4-VP16
                     /note="Gal4-VP16"
                     /translation="ARAMKLLSSIEQACDICRLKKLKCSKEKPKCAKCLKNNWECRYSP
                     KTKRSPLTRAHLTEVESRLERLEQLFLLIFPREDLDMILKMDSLQDIKALLTGLFVQDN
                     VNKDAVTDRLASVETDMPLTLRQHRISATSSSEESSNKGQRQLTVSPEFPGISLIIRFT
                     MPKKKRKVAYSRARTKNNYGSTIEGLLDLPDDDAPEEAGLAAPRLSFLPAGHTRRLSTA
                     PPTDVSLGDELHLDGEDVAMAHADALDDFDLDMLGDGDSPGPGFTPHDSAPYGALDMAD
                     FEFEQMFTDALGIDEYGG"
     misc_structure  2867..3319
                     /label=Gal4-DBD core ORF
                     /note="Gal4-DBD core ORF"
     CDS             2876..3316
                     /codon_start=1
                     /gene="Saccharomyces cerevisiae GAL4 (truncated)"
                     /product="DNA binding domain of the GAL4 transcriptional 
                     activator"
                     /label=GAL4 DNA binding domain
                     /translation="MKLLSSIEQACDICRLKKLKCSKEKPKCAKCLKNNWECRYSPKTK
                     RSPLTRAHLTEVESRLERLEQLFLLIFPREDLDMILKMDSLQDIKALLTGLFVQDNVNK
                     DAVTDRLASVETDMPLTLRQHRISATSSSEESSNKGQRQLTVS"
     misc_structure  3359..3763
                     /label=NLS-VP16
                     /note="NLS-VP16"
     CDS             3359..3379
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             3530..3763
                     /codon_start=1
                     /gene="UL48"
                     /product="transcriptional activation domain of herpes 
                     simplex virus protein VP16 (Triezenberg et al., 1988; 
                     Cousens et al., 1989)"
                     /label=VP16 AD
                     /translation="APPTDVSLGDELHLDGEDVAMAHADALDDFDLDMLGDGDSPGPGF
                     TPHDSAPYGALDMADFEFEQMFTDALGIDEYGG"
     CDS             3530..3763
                     /codon_start=1
                     /gene="UL48"
                     /product="transcriptional activation domain of herpes 
                     simplex virus protein VP16 (Triezenberg et al., 1988; 
                     Cousens et al., 1989)"
                     /label=VP16 AD
                     /translation="APPTDVSLGDELHLDGEDVAMAHADALDDFDLDMLGDGDSPGPGF
                     TPHDSAPYGALDMADFEFEQMFTDALGIDEYGG"
     CDS             3764..3823
                     /codon_start=1
                     /label=2xHA-tag
                     /note="2xHA-tag"
                     /translation="GYPYDVPDYAGYPYDVPDYA"
     CDS             3767..3793
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             3797..3823
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     promoter        complement(3836..3854)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     3874..3891
                     /label=BGH Reverse priming site
                     /note="BGH Reverse priming site"
     polyA_signal    3880..4104
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      4150..4578
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     promoter        4592..4922
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      4773..4908
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             4989..5783
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     polyA_signal    5957..6078
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6127..6143)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    6151..6167
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(6175..6205)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6220..6241
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     rep_origin      complement(6529..7117)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(7288..8148)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(8149..8253)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggac tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gcttggtacc
      901 gagctcggat cccacatcaa caagtttgta caaaaaagca ggctccacca tgaagacgat
      961 catcgccctg agctacatct tctgcctggt attcgccgac tacaaggacg atgatgacgc
     1021 cagcatcgat atgcaattaa atgatgacac caggctctac agtaatgact ttaactctgg
     1081 agaagctaac acttctgatg catttaactg gacagtcgac tctgaaaatc gaaccaacct
     1141 ttcctgtgaa gggtgcctct caccgtcgtg tctctcctta cttcatctcc aggaaaaaaa
     1201 ctggtctgct ttactgacag ccgtagtgat tattctaact attgctggaa acatactcgt
     1261 catcatggca gtgtccctag agaaaaagct gcagaatgcc accaactatt tcctgatgtc
     1321 acttgccata gctgatatgc tgctgggttt ccttgtcatg cccgtgtcca tgttaaccat
     1381 cctgtatggg taccggtggc ctctgccgag caagctttgt gcagtctgga tttacctgga
     1441 cgtgctcttc tccacggcct ccatcatgca cctctgcgcc atctcgctgg atcgctacgt
     1501 cgccatccag aatcccatcc accacagccg cttcaactcc agaactaagg catttctgaa
     1561 aatcattgct gtttggacca tatcagtagg tatatccatg ccaataccag tctttgggct
     1621 acaggacgat tcgaaggtct ttaaggaggg gagttgctta ctcgccgatg ataactttgt
     1681 cctgatcggc tcttttgtgt catttttcat tcccttaacc atcatggtga tcacctactt
     1741 tctaactatc aagtcactcc agaaagaagc tactttgtgt gtaagtgatc ttggcacacg
     1801 ggccaaatta gcttctttca gcttcctccc tcagagttct ttgtcttcag aaaagctctt
     1861 ccagcggtcg atccataggg agccagggtc ctacacaggc aggaggacta tgcagtccat
     1921 cagcaatgag caaaaggcat gcaaggtgct gggcatcgtc ttcttcctgt ttgtggtgat
     1981 gtggtgccct ttcttcatca caaacatcat ggccgtcatc tgcaaagagt cctgcaatga
     2041 ggatgtcatt ggggccctgc tcaatgtgtt tgtttggatc ggttatctct cttcagcagt
     2101 caacccacta gtctacacac tgttcaacaa gacctatagg tcagcctttt cacggtatat
     2161 tcagtgtcag tacaaggaaa acaaaaaacc attgcagtta attttagtga acacaatacc
     2221 ggctttggcc tacaagtcta gccaacttca aatgggacaa aaaaagaatt caaagcaaga
     2281 tgccaagaca acagataatg actgctcaat ggttgctcta ggaaagcagc attctgaaga
     2341 ggcttctaaa gacaatagcg acggagtgaa tgaaaaggtg agctgtgtgg acccagcttt
     2401 cttgtacaaa gtggttgatg tcggaggtgg aggtagcgga ggtggaggta gcggagagag
     2461 cttgtttaag ggaccacgtg attacaaccc gatatcgagc accatttgtc atttgacgaa
     2521 tgaatctgat gggcacacaa catcgttgta tggtattgga tttggtccct tcatcattac
     2581 aaacaagcac ttgtttcgtc gcaataatgg aacactgttg gtccaatcac tacatggtgt
     2641 attcaaggtc aaggacacca cgactttgca acaacacctc attgatggga gggacatgat
     2701 gattattcgc atgcctaagg atttcccacc atttcctcaa aagctgaaat ttagagagcc
     2761 acaaagggaa gagcgcatat gtcttgtgac aaccaacttc caagctcccg agaacctgta
     2821 cttccagggt tctagagaac agaagctgat ctcagaagag gatctggccc gggccatgaa
     2881 gctactgtct tctatcgaac aagcatgcga tatttgccga cttaaaaagc tcaagtgctc
     2941 caaagaaaaa ccgaagtgcg ccaagtgtct gaagaacaac tgggagtgtc gctactctcc
     3001 caaaaccaaa aggtctccgc tgactagggc acatctgaca gaagtggaat caaggctaga
     3061 aagactggaa cagctatttc tactgatttt tcctcgagaa gaccttgaca tgattttgaa
     3121 aatggattct ttacaggata taaaagcatt gttaacagga ttatttgtac aagataatgt
     3181 gaataaagat gccgtcacag atagattggc ttcagtggag actgatatgc ctctaacatt
     3241 gagacagcat agaataagtg cgacatcatc atcggaagag agtagtaaca aaggtcaaag
     3301 acagttgact gtatcgccgg aattcccggg gatcagcttg ataattcgat tcaccatgcc
     3361 caagaagaag aggaaggtgg cgtacagccg cgcgcgtacg aaaaacaatt acgggtctac
     3421 catcgagggc ctgctcgatc tcccggacga cgacgccccc gaagaggcgg ggctggcggc
     3481 tccgcgcctg tcctttctcc ccgcgggaca cacgcgcaga ctgtcgacgg cccccccgac
     3541 cgatgtcagc ctgggggacg agctccactt agacggcgag gacgtcgcga tggcgcatgc
     3601 cgacgcgcta gacgatttcg atctggacat gttgggggac ggggattccc cgggtccggg
     3661 atttaccccc cacgactccg ccccctacgg cgctctggat atggccgact tcgagtttga
     3721 gcagatgttt accgatgccc ttggaattga cgagtacggt gggggctacc catacgatgt
     3781 tcctgactat gcgggctatc cctatgacgt cccggactat gcatgagggc cctattctat
     3841 agtgtcacct aaatgctaga gctcgctgat cagcctcgac tgtgccttct agttgccagc
     3901 catctgttgt ttgcccctcc cccgtgcctt ccttgaccct ggaaggtgcc actcccactg
     3961 tcctttccta ataaaatgag gaaattgcat cgcattgtct gagtaggtgt cattctattc
     4021 tggggggtgg ggtggggcag gacagcaagg gggaggattg ggaagacaat agcaggcatg
     4081 ctggggatgc ggtgggctct atggcttctg aggcggaaag aaccagctgg ggctctaggg
     4141 ggtatcccca cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg gttacgcgca
     4201 gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc tttcgctttc ttcccttcct
     4261 ttctcgccac gttcgccggc tttccccgtc aagctctaaa tcggggcatc cctttagggt
     4321 tccgatttag tgctttacgg cacctcgacc ccaaaaaact tgattagggt gatggttcac
     4381 gtagtgggcc atcgccctga tagacggttt ttcgcccttt gacgttggag tccacgttct
     4441 ttaatagtgg actcttgttc caaactggaa caacactcaa ccctatctcg gtctattctt
     4501 ttgatttata agggattttg gggatttcgg cctattggtt aaaaaatgag ctgatttaac
     4561 aaaaatttaa cgcgaattaa ttctgtggaa tgtgtgtcag ttagggtgtg gaaagtcccc
     4621 aggctcccca ggcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccaggt
     4681 gtggaaagtc cccaggctcc ccagcaggca gaagtatgca aagcatgcat ctcaattagt
     4741 cagcaaccat agtcccgccc ctaactccgc ccatcccgcc cctaactccg cccagttccg
     4801 cccattctcc gccccatggc tgactaattt tttttattta tgcagaggcc gaggccgcct
     4861 ctgcctctga gctattccag aagtagtgag gaggcttttt tggaggccta ggcttttgca
     4921 aaaagctccc gggagcttgt atatccattt tcggatctga tcaagagaca ggatgaggat
     4981 cgtttcgcat gattgaacaa gatggattgc acgcaggttc tccggccgct tgggtggaga
     5041 ggctattcgg ctatgactgg gcacaacaga caatcggctg ctctgatgcc gccgtgttcc
     5101 ggctgtcagc gcaggggcgc ccggttcttt ttgtcaagac cgacctgtcc ggtgccctga
     5161 atgaactgca ggacgaggca gcgcggctat cgtggctggc cacgacgggc gttccttgcg
     5221 cagctgtgct cgacgttgtc actgaagcgg gaagggactg gctgctattg ggcgaagtgc
     5281 cggggcagga tctcctgtca tctcaccttg ctcctgccga gaaagtatcc atcatggctg
     5341 atgcaatgcg gcggctgcat acgcttgatc cggctacctg cccattcgac caccaagcga
     5401 aacatcgcat cgagcgagca cgtactcgga tggaagccgg tcttgtcgat caggatgatc
     5461 tggacgaaga gcatcagggg ctcgcgccag ccgaactgtt cgccaggctc aaggcgcgca
     5521 tgcccgacgg cgaggatctc gtcgtgaccc atggcgatgc ctgcttgccg aatatcatgg
     5581 tggaaaatgg ccgcttttct ggattcatcg actgtggccg gctgggtgtg gcggaccgct
     5641 atcaggacat agcgttggct acccgtgata ttgctgaaga gcttggcggc gaatgggctg
     5701 accgcttcct cgtgctttac ggtatcgccg ctcccgattc gcagcgcatc gccttctatc
     5761 gccttcttga cgagttcttc tgagcgggac tctggggttc gaaatgaccg accaagcgac
     5821 gcccaacctg ccatcacgag atttcgattc caccgccgcc ttctatgaaa ggttgggctt
     5881 cggaatcgtt ttccgggacg ccggctggat gatcctccag cgcggggatc tcatgctgga
     5941 gttcttcgcc caccccaact tgtttattgc agcttataat ggttacaaat aaagcaatag
     6001 catcacaaat ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa
     6061 actcatcaat gtatcttatc atgtctgtat accgtcgacc tctagctaga gcttggcgta
     6121 atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat
     6181 acgagccgga agcataaagt gtaaagcctg gggtgcctaa tgagtgagct aactcacatt
     6241 aattgcgttg cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta
     6301 atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc
     6361 gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa
     6421 ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa
     6481 aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct
     6541 ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac
     6601 aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc
     6661 gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc
     6721 tcaatgctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg
     6781 tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga
     6841 gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta acaggattag
     6901 cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta
     6961 cactagaagg acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag
     7021 agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg
     7081 caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac
     7141 ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc
     7201 aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag
     7261 tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc
     7321 agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac
     7381 gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc
     7441 accggctcca gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg
     7501 tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag
     7561 tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc
     7621 acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac
     7681 atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag
     7741 aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac
     7801 tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg
     7861 agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc
     7921 gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact
     7981 ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg
     8041 atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa
     8101 tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt
     8161 tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg
     8221 tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctga
     8281 cgtc
//
