LOCUS       Exported                8332 bp ds-DNA     circular SYN 11-NOV-2022
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8332)
  AUTHORS   Michael Wehr
  TITLE     Direct Submission
  JOURNAL   Exported Monday, Nov 14, 2022 from SnapGene 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..8332
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     promoter        209..863
                     /label=CMV promotor
                     /note="CMV promotor"
     enhancer        235..614
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     misc_feature    440
                     /label=C->G
     promoter        615..818
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     863..882
                     /label=T7 promoter/priming site
                     /note="T7 promoter/priming site"
     promoter        863..881
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     misc_feature    895..906
                     /gene="miscellaneous"
                     /label=reading Frame B
                     /note="reading Frame B"
                     /note="/SECDrawas=Region"
     protein_bind    899..923
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             929..2341
                     /codon_start=1
                     /gene="hHTR2A-var1-Cop"
                     /label=hHTR2A-var1-Cop
                     /note="accession: NM_000621"
                     /note="/SECDrawas=Gene"
                     /note="/organism=Homo Sapiens"
                     /translation="MDILCEENTSLSSTTNSLMQLNDDTRLYSNDFNSGEANTSDAFNW
                     TVDSENRTNLSCEGCLSPSCLSLLHLQEKNWSALLTAVVIILTIAGNILVIMAVSLEKK
                     LQNATNYFLMSLAIADMLLGFLVMPVSMLTILYGYRWPLPSKLCAVWIYLDVLFSTASI
                     MHLCAISLDRYVAIQNPIHHSRFNSRTKAFLKIIAVWTISVGISMPIPVFGLQDDSKVF
                     KEGSCLLADDNFVLIGSFVSFFIPLTIMVITYFLTIKSLQKEATLCVSDLGTRAKLASF
                     SFLPQSSLSSEKLFQRSIHREPGSYTGRRTMQSISNEQKACKVLGIVFFLFVVMWCPFF
                     ITNIMAVICKESCNEDVIGALLNVFVWIGYLSSAVNPLVYTLFNKTYRSAFSRYIQCQY
                     KENKKPLQLILVNTIPALAYKSSQLQMGQKKNSKQDAKTTDNDCSMVALGKQHSEEASK
                     DNSDGVNEKVSCV"
     protein_bind    complement(2343..2367)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    2353..2371
                     /gene="miscellaneous"
                     /label=reading Frame B
                     /note="reading Frame B"
                     /note="/SECDrawas=Region"
     CDS             2378..2464
                     /codon_start=1
                     /gene="coding sequence"
                     /label=V2R aa343-371
                     /note="V2R aa343-371"
                     /note="/SECDrawas=Region"
                     /translation="ARGRTPPSLGPQDESCTTASSSLAKDTSS"
     CDS             2471..2500
                     /codon_start=1
                     /label=GS linker
                     /note="GS linker"
                     /translation="GGGGSGGGGS"
     CDS             2501..2854
                     /codon_start=1
                     /label=TEV1-118
                     /note="TEV1-118"
                     /translation="GESLFKGPRDYNPISSTICHLTNESDGHTTSLYGIGFGPFIITNK
                     HLFRRNNGTLLVQSLHGVFKVKDTTTLQQHLIDGRDMMIIRMPKDFPPFPQKLKFREPQ
                     REERICLVTTNFQA"
     misc_binding    complement(2734..2756)
                     /label=NTEV 5' sequencing primer
                     /note="NTEV 5' sequencing primer"
     CDS             2858..2878
                     /codon_start=1
                     /label=tevS
                     /note="tevS (TEV cleavage site)"
                     /translation="ENLYFQG"
     CDS             2858..2878
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             2885..2914
                     /codon_start=1
                     /label=Myc-tag
                     /note="Myc-tag (due to cloning reasons - taken from old 
                     pDEST_X-ASPlink-NTEV-tevS-GV which contained that Myc-tag 
                     already)"
                     /translation="EQKLISEEDL"
     CDS             2885..2914
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             2915..3811
                     /codon_start=1
                     /label=Gal4-VP16
                     /note="Gal4-VP16"
                     /translation="ARAMKLLSSIEQACDICRLKKLKCSKEKPKCAKCLKNNWECRYSP
                     KTKRSPLTRAHLTEVESRLERLEQLFLLIFPREDLDMILKMDSLQDIKALLTGLFVQDN
                     VNKDAVTDRLASVETDMPLTLRQHRISATSSSEESSNKGQRQLTVSPEFPGISLIIRFT
                     MPKKKRKVAYSRARTKNNYGSTIEGLLDLPDDDAPEEAGLAAPRLSFLPAGHTRRLSTA
                     PPTDVSLGDELHLDGEDVAMAHADALDDFDLDMLGDGDSPGPGFTPHDSAPYGALDMAD
                     FEFEQMFTDALGIDEYGG"
     misc_structure  2915..3367
                     /label=Gal4-DBD core ORF
                     /note="Gal4-DBD core ORF"
     CDS             2924..3364
                     /codon_start=1
                     /gene="Saccharomyces cerevisiae GAL4 (truncated)"
                     /product="DNA binding domain of the GAL4 transcriptional 
                     activator"
                     /label=GAL4 DNA binding domain
                     /translation="MKLLSSIEQACDICRLKKLKCSKEKPKCAKCLKNNWECRYSPKTK
                     RSPLTRAHLTEVESRLERLEQLFLLIFPREDLDMILKMDSLQDIKALLTGLFVQDNVNK
                     DAVTDRLASVETDMPLTLRQHRISATSSSEESSNKGQRQLTVS"
     misc_structure  3407..3811
                     /label=NLS-VP16
                     /note="NLS-VP16"
     CDS             3407..3427
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             3578..3811
                     /codon_start=1
                     /gene="UL48"
                     /product="transcriptional activation domain of herpes 
                     simplex virus protein VP16 (Triezenberg et al., 1988; 
                     Cousens et al., 1989)"
                     /label=VP16 AD
                     /translation="APPTDVSLGDELHLDGEDVAMAHADALDDFDLDMLGDGDSPGPGF
                     TPHDSAPYGALDMADFEFEQMFTDALGIDEYGG"
     CDS             3812..3871
                     /codon_start=1
                     /label=2xHA-tag
                     /note="2xHA-tag"
                     /translation="GYPYDVPDYAGYPYDVPDYA"
     CDS             3815..3841
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             3845..3871
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     promoter        complement(3884..3902)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     polyA_site      3904..4135
                     /label=BGH polyA
                     /note="BGH polyA"
     primer_bind     3922..3939
                     /label=BGH Reverse priming site
                     /note="BGH Reverse priming site"
     polyA_signal    3928..4152
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      4198..4626
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     promoter        4640..4970
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      4821..4956
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             5037..5831
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     polyA_signal    5886..6258
                     /label=SV40 poly A
                     /note="SV40 poly A"
     polyA_signal    6005..6126
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6175..6191)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    6199..6215
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(6223..6253)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6268..6289
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     rep_origin      complement(6518..7191)
                     /direction=LEFT
                     /label=ColE1 origin (pBR3222 origin)
                     /note="ColE1 origin (pBR3222 origin)"
     rep_origin      complement(6577..7165)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(7336..8196)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(8197..8301)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gcttatcaac
      901 aagtttgtac aaaaaagcag gctctaccat ggatattctt tgtgaagaaa atacttcttt
      961 gagctcaact acgaactccc taatgcaatt aaatgatgac accaggctct acagtaatga
     1021 ctttaactct ggagaagcta acacttctga tgcatttaac tggacagtcg actctgaaaa
     1081 tcgaaccaac ctttcctgtg aagggtgcct ctcaccgtcg tgtctctcct tacttcatct
     1141 ccaggaaaaa aactggtctg ctttactgac agccgtagtg attattctaa ctattgctgg
     1201 aaacatactc gtcatcatgg cagtgtccct agagaaaaag ctgcagaatg ccaccaacta
     1261 tttcctgatg tcacttgcca tagctgatat gctgctgggt ttccttgtca tgcccgtgtc
     1321 catgttaacc atcctgtatg ggtaccggtg gcctctgccg agcaagcttt gtgcagtctg
     1381 gatttacctg gacgtgctct tctccacggc ctccatcatg cacctctgcg ccatctcgct
     1441 ggatcgctac gtcgccatcc agaatcccat ccaccacagc cgcttcaact ccagaactaa
     1501 ggcatttctg aaaatcattg ctgtttggac catatcagta ggtatatcca tgccaatacc
     1561 agtctttggg ctacaggacg attcgaaggt ctttaaggag gggagttgct tactcgccga
     1621 tgataacttt gtcctgatcg gctcttttgt gtcatttttc attcccttaa ccatcatggt
     1681 gatcacctac tttctaacta tcaagtcact ccagaaagaa gctactttgt gtgtaagtga
     1741 tcttggcaca cgggccaaat tagcttcttt cagcttcctc cctcagagtt ctttgtcttc
     1801 agaaaagctc ttccagcggt cgatccatag ggagccaggg tcctacacag gcaggaggac
     1861 tatgcagtcc atcagcaatg agcaaaaggc atgcaaggtg ctgggcatcg tcttcttcct
     1921 gtttgtggtg atgtggtgcc ctttcttcat cacaaacatc atggccgtca tctgcaaaga
     1981 gtcctgcaat gaggatgtca ttggggccct gctcaatgtg tttgtttgga tcggttatct
     2041 ctcttcagca gtcaacccac tagtctacac actgttcaac aagacctata ggtcagcctt
     2101 ttcacggtat attcagtgtc agtacaagga aaacaaaaaa ccattgcagt taattttagt
     2161 gaacacaata ccggctttgg cctacaagtc tagccaactt caaatgggac aaaaaaagaa
     2221 ttcaaagcaa gatgccaaga caacagataa tgactgctca atggttgctc taggaaagca
     2281 gcattctgaa gaggcttcta aagacaatag cgacggagtg aatgaaaagg tgagctgtgt
     2341 ggacccagct ttcttgtaca aagtggttga tggtaccgcc cggggacgca ccccacccag
     2401 cctgggtccc caagatgagt cctgcaccac cgccagctcc tccctggcca aggacacttc
     2461 atcggatgtc ggaggtggag gtagcggagg tggaggtagc ggagagagct tgtttaaggg
     2521 accacgtgat tacaacccga tatcgagcac catttgtcat ttgacgaatg aatctgatgg
     2581 gcacacaaca tcgttgtatg gtattggatt tggtcccttc atcattacaa acaagcactt
     2641 gtttcgtcgc aataatggaa cactgttggt ccaatcacta catggtgtat tcaaggtcaa
     2701 ggacaccacg actttgcaac aacacctcat tgatgggagg gacatgatga ttattcgcat
     2761 gcctaaggat ttcccaccat ttcctcaaaa gctgaaattt agagagccac aaagggaaga
     2821 gcgcatatgt cttgtgacaa ccaacttcca agctcccgag aacctgtact tccagggttc
     2881 tagagaacag aagctgatct cagaagagga tctggcccgg gccatgaagc tactgtcttc
     2941 tatcgaacaa gcatgcgata tttgccgact taaaaagctc aagtgctcca aagaaaaacc
     3001 gaagtgcgcc aagtgtctga agaacaactg ggagtgtcgc tactctccca aaaccaaaag
     3061 gtctccgctg actagggcac atctgacaga agtggaatca aggctagaaa gactggaaca
     3121 gctatttcta ctgatttttc ctcgagaaga ccttgacatg attttgaaaa tggattcttt
     3181 acaggatata aaagcattgt taacaggatt atttgtacaa gataatgtga ataaagatgc
     3241 cgtcacagat agattggctt cagtggagac tgatatgcct ctaacattga gacagcatag
     3301 aataagtgcg acatcatcat cggaagagag tagtaacaaa ggtcaaagac agttgactgt
     3361 atcgccggaa ttcccgggga tcagcttgat aattcgattc accatgccca agaagaagag
     3421 gaaggtggcg tacagccgcg cgcgtacgaa aaacaattac gggtctacca tcgagggcct
     3481 gctcgatctc ccggacgacg acgcccccga agaggcgggg ctggcggctc cgcgcctgtc
     3541 ctttctcccc gcgggacaca cgcgcagact gtcgacggcc cccccgaccg atgtcagcct
     3601 gggggacgag ctccacttag acggcgagga cgtcgcgatg gcgcatgccg acgcgctaga
     3661 cgatttcgat ctggacatgt tgggggacgg ggattccccg ggtccgggat ttacccccca
     3721 cgactccgcc ccctacggcg ctctggatat ggccgacttc gagtttgagc agatgtttac
     3781 cgatgccctt ggaattgacg agtacggtgg gggctaccca tacgatgttc ctgactatgc
     3841 gggctatccc tatgacgtcc cggactatgc atgagggccc tattctatag tgtcacctaa
     3901 atgctagagc tcgctgatca gcctcgactg tgccttctag ttgccagcca tctgttgttt
     3961 gcccctcccc cgtgccttcc ttgaccctgg aaggtgccac tcccactgtc ctttcctaat
     4021 aaaatgagga aattgcatcg cattgtctga gtaggtgtca ttctattctg gggggtgggg
     4081 tggggcagga cagcaagggg gaggattggg aagacaatag caggcatgct ggggatgcgg
     4141 tgggctctat ggcttctgag gcggaaagaa ccagctgggg ctctaggggg tatccccacg
     4201 cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta
     4261 cacttgccag cgccctagcg cccgctcctt tcgctttctt cccttccttt ctcgccacgt
     4321 tcgccggctt tccccgtcaa gctctaaatc ggggcatccc tttagggttc cgatttagtg
     4381 ctttacggca cctcgacccc aaaaaacttg attagggtga tggttcacgt agtgggccat
     4441 cgccctgata gacggttttt cgccctttga cgttggagtc cacgttcttt aatagtggac
     4501 tcttgttcca aactggaaca acactcaacc ctatctcggt ctattctttt gatttataag
     4561 ggattttggg gatttcggcc tattggttaa aaaatgagct gatttaacaa aaatttaacg
     4621 cgaattaatt ctgtggaatg tgtgtcagtt agggtgtgga aagtccccag gctccccagg
     4681 caggcagaag tatgcaaagc atgcatctca attagtcagc aaccaggtgt ggaaagtccc
     4741 caggctcccc agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccatag
     4801 tcccgcccct aactccgccc atcccgcccc taactccgcc cagttccgcc cattctccgc
     4861 cccatggctg actaattttt tttatttatg cagaggccga ggccgcctct gcctctgagc
     4921 tattccagaa gtagtgagga ggcttttttg gaggcctagg cttttgcaaa aagctcccgg
     4981 gagcttgtat atccattttc ggatctgatc aagagacagg atgaggatcg tttcgcatga
     5041 ttgaacaaga tggattgcac gcaggttctc cggccgcttg ggtggagagg ctattcggct
     5101 atgactgggc acaacagaca atcggctgct ctgatgccgc cgtgttccgg ctgtcagcgc
     5161 aggggcgccc ggttcttttt gtcaagaccg acctgtccgg tgccctgaat gaactgcagg
     5221 acgaggcagc gcggctatcg tggctggcca cgacgggcgt tccttgcgca gctgtgctcg
     5281 acgttgtcac tgaagcggga agggactggc tgctattggg cgaagtgccg gggcaggatc
     5341 tcctgtcatc tcaccttgct cctgccgaga aagtatccat catggctgat gcaatgcggc
     5401 ggctgcatac gcttgatccg gctacctgcc cattcgacca ccaagcgaaa catcgcatcg
     5461 agcgagcacg tactcggatg gaagccggtc ttgtcgatca ggatgatctg gacgaagagc
     5521 atcaggggct cgcgccagcc gaactgttcg ccaggctcaa ggcgcgcatg cccgacggcg
     5581 aggatctcgt cgtgacccat ggcgatgcct gcttgccgaa tatcatggtg gaaaatggcc
     5641 gcttttctgg attcatcgac tgtggccggc tgggtgtggc ggaccgctat caggacatag
     5701 cgttggctac ccgtgatatt gctgaagagc ttggcggcga atgggctgac cgcttcctcg
     5761 tgctttacgg tatcgccgct cccgattcgc agcgcatcgc cttctatcgc cttcttgacg
     5821 agttcttctg agcgggactc tggggttcga aatgaccgac caagcgacgc ccaacctgcc
     5881 atcacgagat ttcgattcca ccgccgcctt ctatgaaagg ttgggcttcg gaatcgtttt
     5941 ccgggacgcc ggctggatga tcctccagcg cggggatctc atgctggagt tcttcgccca
     6001 ccccaacttg tttattgcag cttataatgg ttacaaataa agcaatagca tcacaaattt
     6061 cacaaataaa gcattttttt cactgcattc tagttgtggt ttgtccaaac tcatcaatgt
     6121 atcttatcat gtctgtatac cgtcgacctc tagctagagc ttggcgtaat catggtcata
     6181 gctgtttcct gtgtgaaatt gttatccgct cacaattcca cacaacatac gagccggaag
     6241 cataaagtgt aaagcctggg gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg
     6301 ctcactgccc gctttccagt cgggaaacct gtcgtgccag ctgcattaat gaatcggcca
     6361 acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc
     6421 gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg
     6481 gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa
     6541 ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga
     6601 cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag
     6661 ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct
     6721 taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc aatgctcacg
     6781 ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc
     6841 ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt
     6901 aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta
     6961 tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaaggac
     7021 agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc
     7081 ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat
     7141 tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc
     7201 tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt
     7261 cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta
     7321 aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct
     7381 atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg
     7441 cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga
     7501 tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt
     7561 atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt
     7621 taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt
     7681 tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat
     7741 gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc
     7801 cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc
     7861 cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat
     7921 gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag
     7981 aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt
     8041 accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc
     8101 ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa
     8161 gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg
     8221 aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa
     8281 taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg tc
//
