LOCUS       Exported                8359 bp ds-DNA     circular SYN 11-NOV-2022
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8359)
  AUTHORS   Michael Wehr
  TITLE     Direct Submission
  JOURNAL   Exported Monday, Nov 14, 2022 from SnapGene 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..8359
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     promoter        209..863
                     /label=CMV promotor
                     /note="CMV promotor"
     enhancer        235..614
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     misc_feature    440
                     /label=C->G
     promoter        615..818
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     863..882
                     /label=T7 promoter/priming site
                     /note="T7 promoter/priming site"
     promoter        863..881
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     misc_feature    895..906
                     /gene="miscellaneous"
                     /label=reading Frame B
                     /note="reading Frame B"
                     /note="/SECDrawas=Region"
     protein_bind    899..923
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    924..1009
                     /label=TANGO Presto 5' Signal peptide
     CDS             929..976
                     /codon_start=1
                     /label=HA1 signal peptide
                     /note="Hemagglutinin HA1 chain signal peptideInfluenza A 
                     virus
                     "
                     /translation="MKTIIALSYIFCLVFA"
     CDS             977..1000
                     /codon_start=1
                     /product="Note that the FLAG tag is not functional due to a
                     K->A mutation.
                     "
                     /label=FLAG_K->A
                     /note="C-terminal K -> A
                     FLAG-tag: DYKDDDDK
                     "
                     /translation="DYKDDDDA"
     CDS             1013..2368
                     /codon_start=1
                     /product="NM_000621"
                     /label=hHTR2A
                     /note="hHTR2A"
                     /translation="QLNDDTRLYSNDFNSGEANTSDAFNWTVDSENRTNLSCEGCLSPS
                     CLSLLHLQEKNWSALLTAVVIILTIAGNILVIMAVSLEKKLQNATNYFLMSLAIADMLL
                     GFLVMPVSMLTILYGYRWPLPSKLCAVWIYLDVLFSTASIMHLCAISLDRYVAIQNPIH
                     HSRFNSRTKAFLKIIAVWTISVGISMPIPVFGLQDDSKVFKEGSCLLADDNFVLIGSFV
                     SFFIPLTIMVITYFLTIKSLQKEATLCVSDLGTRAKLASFSFLPQSSLSSEKLFQRSIH
                     REPGSYTGRRTMQSISNEQKACKVLGIVFFLFVVMWCPFFITNIMAVICKESCNEDVIG
                     ALLNVFVWIGYLSSAVNPLVYTLFNKTYRSAFSRYIQCQYKENKKPLQLILVNTIPALA
                     YKSSQLQMGQKKNSKQDAKTTDNDCSMVALGKQHSEEASKDNSDGVNEKVSCV"
     protein_bind    complement(2370..2394)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    2380..2398
                     /gene="miscellaneous"
                     /label=reading Frame B
                     /note="reading Frame B"
                     /note="/SECDrawas=Region"
     CDS             2405..2491
                     /codon_start=1
                     /gene="coding sequence"
                     /label=V2R aa343-371
                     /note="V2R aa343-371"
                     /note="/SECDrawas=Region"
                     /translation="ARGRTPPSLGPQDESCTTASSSLAKDTSS"
     CDS             2498..2527
                     /codon_start=1
                     /label=GS linker
                     /note="GS linker"
                     /translation="GGGGSGGGGS"
     CDS             2528..2881
                     /codon_start=1
                     /label=TEV1-118
                     /note="TEV1-118"
                     /translation="GESLFKGPRDYNPISSTICHLTNESDGHTTSLYGIGFGPFIITNK
                     HLFRRNNGTLLVQSLHGVFKVKDTTTLQQHLIDGRDMMIIRMPKDFPPFPQKLKFREPQ
                     REERICLVTTNFQA"
     misc_binding    complement(2761..2783)
                     /label=NTEV 5' sequencing primer
                     /note="NTEV 5' sequencing primer"
     CDS             2885..2905
                     /codon_start=1
                     /label=tevS
                     /note="tevS (TEV cleavage site)"
                     /translation="ENLYFQG"
     CDS             2885..2905
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             2912..2941
                     /codon_start=1
                     /label=Myc-tag
                     /note="Myc-tag (due to cloning reasons - taken from old 
                     pDEST_X-ASPlink-NTEV-tevS-GV which contained that Myc-tag 
                     already)"
                     /translation="EQKLISEEDL"
     CDS             2912..2941
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             2942..3838
                     /codon_start=1
                     /label=Gal4-VP16
                     /note="Gal4-VP16"
                     /translation="ARAMKLLSSIEQACDICRLKKLKCSKEKPKCAKCLKNNWECRYSP
                     KTKRSPLTRAHLTEVESRLERLEQLFLLIFPREDLDMILKMDSLQDIKALLTGLFVQDN
                     VNKDAVTDRLASVETDMPLTLRQHRISATSSSEESSNKGQRQLTVSPEFPGISLIIRFT
                     MPKKKRKVAYSRARTKNNYGSTIEGLLDLPDDDAPEEAGLAAPRLSFLPAGHTRRLSTA
                     PPTDVSLGDELHLDGEDVAMAHADALDDFDLDMLGDGDSPGPGFTPHDSAPYGALDMAD
                     FEFEQMFTDALGIDEYGG"
     misc_structure  2942..3394
                     /label=Gal4-DBD core ORF
                     /note="Gal4-DBD core ORF"
     CDS             2951..3391
                     /codon_start=1
                     /gene="Saccharomyces cerevisiae GAL4 (truncated)"
                     /product="DNA binding domain of the GAL4 transcriptional 
                     activator"
                     /label=GAL4 DNA binding domain
                     /translation="MKLLSSIEQACDICRLKKLKCSKEKPKCAKCLKNNWECRYSPKTK
                     RSPLTRAHLTEVESRLERLEQLFLLIFPREDLDMILKMDSLQDIKALLTGLFVQDNVNK
                     DAVTDRLASVETDMPLTLRQHRISATSSSEESSNKGQRQLTVS"
     misc_structure  3434..3838
                     /label=NLS-VP16
                     /note="NLS-VP16"
     CDS             3434..3454
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             3605..3838
                     /codon_start=1
                     /gene="UL48"
                     /product="transcriptional activation domain of herpes 
                     simplex virus protein VP16 (Triezenberg et al., 1988; 
                     Cousens et al., 1989)"
                     /label=VP16 AD
                     /translation="APPTDVSLGDELHLDGEDVAMAHADALDDFDLDMLGDGDSPGPGF
                     TPHDSAPYGALDMADFEFEQMFTDALGIDEYGG"
     CDS             3839..3898
                     /codon_start=1
                     /label=2xHA-tag
                     /note="2xHA-tag"
                     /translation="GYPYDVPDYAGYPYDVPDYA"
     CDS             3842..3868
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             3872..3898
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     promoter        complement(3911..3929)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     polyA_site      3931..4162
                     /label=BGH polyA
                     /note="BGH polyA"
     primer_bind     3949..3966
                     /label=BGH Reverse priming site
                     /note="BGH Reverse priming site"
     polyA_signal    3955..4179
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      4225..4653
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     promoter        4667..4997
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      4848..4983
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             5064..5858
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     polyA_signal    5913..6285
                     /label=SV40 poly A
                     /note="SV40 poly A"
     polyA_signal    6032..6153
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6202..6218)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    6226..6242
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(6250..6280)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6295..6316
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     rep_origin      complement(6545..7218)
                     /direction=LEFT
                     /label=ColE1 origin (pBR3222 origin)
                     /note="ColE1 origin (pBR3222 origin)"
     rep_origin      complement(6604..7192)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(7363..8223)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(8224..8328)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gcttatcaac
      901 aagtttgtac aaaaaagcag gctccaccat gaagacgatc atcgccctga gctacatctt
      961 ctgcctggta ttcgccgact acaaggacga tgatgacgcc agcatcgata tgcaattaaa
     1021 tgatgacacc aggctctaca gtaatgactt taactctgga gaagctaaca cttctgatgc
     1081 atttaactgg acagtcgact ctgaaaatcg aaccaacctt tcctgtgaag ggtgcctctc
     1141 accgtcgtgt ctctccttac ttcatctcca ggaaaaaaac tggtctgctt tactgacagc
     1201 cgtagtgatt attctaacta ttgctggaaa catactcgtc atcatggcag tgtccctaga
     1261 gaaaaagctg cagaatgcca ccaactattt cctgatgtca cttgccatag ctgatatgct
     1321 gctgggtttc cttgtcatgc ccgtgtccat gttaaccatc ctgtatgggt accggtggcc
     1381 tctgccgagc aagctttgtg cagtctggat ttacctggac gtgctcttct ccacggcctc
     1441 catcatgcac ctctgcgcca tctcgctgga tcgctacgtc gccatccaga atcccatcca
     1501 ccacagccgc ttcaactcca gaactaaggc atttctgaaa atcattgctg tttggaccat
     1561 atcagtaggt atatccatgc caataccagt ctttgggcta caggacgatt cgaaggtctt
     1621 taaggagggg agttgcttac tcgccgatga taactttgtc ctgatcggct cttttgtgtc
     1681 atttttcatt cccttaacca tcatggtgat cacctacttt ctaactatca agtcactcca
     1741 gaaagaagct actttgtgtg taagtgatct tggcacacgg gccaaattag cttctttcag
     1801 cttcctccct cagagttctt tgtcttcaga aaagctcttc cagcggtcga tccataggga
     1861 gccagggtcc tacacaggca ggaggactat gcagtccatc agcaatgagc aaaaggcatg
     1921 caaggtgctg ggcatcgtct tcttcctgtt tgtggtgatg tggtgccctt tcttcatcac
     1981 aaacatcatg gccgtcatct gcaaagagtc ctgcaatgag gatgtcattg gggccctgct
     2041 caatgtgttt gtttggatcg gttatctctc ttcagcagtc aacccactag tctacacact
     2101 gttcaacaag acctataggt cagccttttc acggtatatt cagtgtcagt acaaggaaaa
     2161 caaaaaacca ttgcagttaa ttttagtgaa cacaataccg gctttggcct acaagtctag
     2221 ccaacttcaa atgggacaaa aaaagaattc aaagcaagat gccaagacaa cagataatga
     2281 ctgctcaatg gttgctctag gaaagcagca ttctgaagag gcttctaaag acaatagcga
     2341 cggagtgaat gaaaaggtga gctgtgtgga cccagctttc ttgtacaaag tggttgatgg
     2401 taccgcccgg ggacgcaccc cacccagcct gggtccccaa gatgagtcct gcaccaccgc
     2461 cagctcctcc ctggccaagg acacttcatc ggatgtcgga ggtggaggta gcggaggtgg
     2521 aggtagcgga gagagcttgt ttaagggacc acgtgattac aacccgatat cgagcaccat
     2581 ttgtcatttg acgaatgaat ctgatgggca cacaacatcg ttgtatggta ttggatttgg
     2641 tcccttcatc attacaaaca agcacttgtt tcgtcgcaat aatggaacac tgttggtcca
     2701 atcactacat ggtgtattca aggtcaagga caccacgact ttgcaacaac acctcattga
     2761 tgggagggac atgatgatta ttcgcatgcc taaggatttc ccaccatttc ctcaaaagct
     2821 gaaatttaga gagccacaaa gggaagagcg catatgtctt gtgacaacca acttccaagc
     2881 tcccgagaac ctgtacttcc agggttctag agaacagaag ctgatctcag aagaggatct
     2941 ggcccgggcc atgaagctac tgtcttctat cgaacaagca tgcgatattt gccgacttaa
     3001 aaagctcaag tgctccaaag aaaaaccgaa gtgcgccaag tgtctgaaga acaactggga
     3061 gtgtcgctac tctcccaaaa ccaaaaggtc tccgctgact agggcacatc tgacagaagt
     3121 ggaatcaagg ctagaaagac tggaacagct atttctactg atttttcctc gagaagacct
     3181 tgacatgatt ttgaaaatgg attctttaca ggatataaaa gcattgttaa caggattatt
     3241 tgtacaagat aatgtgaata aagatgccgt cacagataga ttggcttcag tggagactga
     3301 tatgcctcta acattgagac agcatagaat aagtgcgaca tcatcatcgg aagagagtag
     3361 taacaaaggt caaagacagt tgactgtatc gccggaattc ccggggatca gcttgataat
     3421 tcgattcacc atgcccaaga agaagaggaa ggtggcgtac agccgcgcgc gtacgaaaaa
     3481 caattacggg tctaccatcg agggcctgct cgatctcccg gacgacgacg cccccgaaga
     3541 ggcggggctg gcggctccgc gcctgtcctt tctccccgcg ggacacacgc gcagactgtc
     3601 gacggccccc ccgaccgatg tcagcctggg ggacgagctc cacttagacg gcgaggacgt
     3661 cgcgatggcg catgccgacg cgctagacga tttcgatctg gacatgttgg gggacgggga
     3721 ttccccgggt ccgggattta ccccccacga ctccgccccc tacggcgctc tggatatggc
     3781 cgacttcgag tttgagcaga tgtttaccga tgcccttgga attgacgagt acggtggggg
     3841 ctacccatac gatgttcctg actatgcggg ctatccctat gacgtcccgg actatgcatg
     3901 agggccctat tctatagtgt cacctaaatg ctagagctcg ctgatcagcc tcgactgtgc
     3961 cttctagttg ccagccatct gttgtttgcc cctcccccgt gccttccttg accctggaag
     4021 gtgccactcc cactgtcctt tcctaataaa atgaggaaat tgcatcgcat tgtctgagta
     4081 ggtgtcattc tattctgggg ggtggggtgg ggcaggacag caagggggag gattgggaag
     4141 acaatagcag gcatgctggg gatgcggtgg gctctatggc ttctgaggcg gaaagaacca
     4201 gctggggctc tagggggtat ccccacgcgc cctgtagcgg cgcattaagc gcggcgggtg
     4261 tggtggttac gcgcagcgtg accgctacac ttgccagcgc cctagcgccc gctcctttcg
     4321 ctttcttccc ttcctttctc gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg
     4381 gcatcccttt agggttccga tttagtgctt tacggcacct cgaccccaaa aaacttgatt
     4441 agggtgatgg ttcacgtagt gggccatcgc cctgatagac ggtttttcgc cctttgacgt
     4501 tggagtccac gttctttaat agtggactct tgttccaaac tggaacaaca ctcaacccta
     4561 tctcggtcta ttcttttgat ttataaggga ttttggggat ttcggcctat tggttaaaaa
     4621 atgagctgat ttaacaaaaa tttaacgcga attaattctg tggaatgtgt gtcagttagg
     4681 gtgtggaaag tccccaggct ccccaggcag gcagaagtat gcaaagcatg catctcaatt
     4741 agtcagcaac caggtgtgga aagtccccag gctccccagc aggcagaagt atgcaaagca
     4801 tgcatctcaa ttagtcagca accatagtcc cgcccctaac tccgcccatc ccgcccctaa
     4861 ctccgcccag ttccgcccat tctccgcccc atggctgact aatttttttt atttatgcag
     4921 aggccgaggc cgcctctgcc tctgagctat tccagaagta gtgaggaggc ttttttggag
     4981 gcctaggctt ttgcaaaaag ctcccgggag cttgtatatc cattttcgga tctgatcaag
     5041 agacaggatg aggatcgttt cgcatgattg aacaagatgg attgcacgca ggttctccgg
     5101 ccgcttgggt ggagaggcta ttcggctatg actgggcaca acagacaatc ggctgctctg
     5161 atgccgccgt gttccggctg tcagcgcagg ggcgcccggt tctttttgtc aagaccgacc
     5221 tgtccggtgc cctgaatgaa ctgcaggacg aggcagcgcg gctatcgtgg ctggccacga
     5281 cgggcgttcc ttgcgcagct gtgctcgacg ttgtcactga agcgggaagg gactggctgc
     5341 tattgggcga agtgccgggg caggatctcc tgtcatctca ccttgctcct gccgagaaag
     5401 tatccatcat ggctgatgca atgcggcggc tgcatacgct tgatccggct acctgcccat
     5461 tcgaccacca agcgaaacat cgcatcgagc gagcacgtac tcggatggaa gccggtcttg
     5521 tcgatcagga tgatctggac gaagagcatc aggggctcgc gccagccgaa ctgttcgcca
     5581 ggctcaaggc gcgcatgccc gacggcgagg atctcgtcgt gacccatggc gatgcctgct
     5641 tgccgaatat catggtggaa aatggccgct tttctggatt catcgactgt ggccggctgg
     5701 gtgtggcgga ccgctatcag gacatagcgt tggctacccg tgatattgct gaagagcttg
     5761 gcggcgaatg ggctgaccgc ttcctcgtgc tttacggtat cgccgctccc gattcgcagc
     5821 gcatcgcctt ctatcgcctt cttgacgagt tcttctgagc gggactctgg ggttcgaaat
     5881 gaccgaccaa gcgacgccca acctgccatc acgagatttc gattccaccg ccgccttcta
     5941 tgaaaggttg ggcttcggaa tcgttttccg ggacgccggc tggatgatcc tccagcgcgg
     6001 ggatctcatg ctggagttct tcgcccaccc caacttgttt attgcagctt ataatggtta
     6061 caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac tgcattctag
     6121 ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc tgtataccgt cgacctctag
     6181 ctagagcttg gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt atccgctcac
     6241 aattccacac aacatacgag ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt
     6301 gagctaactc acattaattg cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc
     6361 gtgccagctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg
     6421 ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt
     6481 atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa
     6541 gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc
     6601 gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag
     6661 gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt
     6721 gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg
     6781 aagcgtggcg ctttctcaat gctcacgctg taggtatctc agttcggtgt aggtcgttcg
     6841 ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg
     6901 taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac
     6961 tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg
     7021 gcctaactac ggctacacta gaaggacagt atttggtatc tgcgctctgc tgaagccagt
     7081 taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg
     7141 tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc
     7201 tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt
     7261 ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt
     7321 taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag
     7381 tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt
     7441 cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg caatgatacc
     7501 gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc
     7561 cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg
     7621 ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac
     7681 aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg
     7741 atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc
     7801 tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact
     7861 gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc
     7921 aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat
     7981 acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc
     8041 ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac
     8101 tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa
     8161 aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact
     8221 catactcttc ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg
     8281 atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg
     8341 aaaagtgcca cctgacgtc
//
