LOCUS       Exported                8275 bp ds-DNA     circular SYN 11-NOV-2022
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8275)
  AUTHORS   Michael Wehr
  TITLE     Direct Submission
  JOURNAL   Exported Monday, Nov 14, 2022 from SnapGene 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..8275
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     enhancer        235..614
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        615..818
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     863..882
                     /label=T7 promoter/priming site
                     /note="T7 promoter/priming site"
     promoter        863..881
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     misc_feature    916..927
                     /label=rfB
                     /note="rfB"
     protein_bind    920..944
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             950..2380
                     /codon_start=1
                     /label=GCGR Cop, NM_000160
                     /note="GL-R"
                     /translation="MPPCQPQRPLLLLLLLLACQPQVPSAQVMDFLFEKWKLYGDQCHH
                     NLSLLPPPTELVCNRTFDKYSCWPDTPANTTANISCPWYLPWHHKVQHRFVFKRCGPDG
                     QWVRGPRGQPWRDASQCQMDGEEIEVQKEVAKMYSSFQVMYTVGYSLSLGALLLALAIL
                     GGLSKLHCTRNAIHANLFASFVLKASSVLVIDGLLRTRYSQKIGDDLSVSTWLSDGAVA
                     GCRVAAVFMQYGIVANYCWLLVEGLYLHNLLGLATLPERSFFSLYLGIGWGAPMLFVVP
                     WAVVKCLFENVQCWTSNDNMGFWWILRFPVFLAILINFFIFVRIVQLLVAKLRARQMHH
                     TDYKFRLAKSTLTLIPLLGVHEVVFAFVTDEHAQGTLRSAKLFFDLFLSSFQGLLVAVL
                     YCFLNKEVQSELRRRWHRWRLGKVLWEERNTSNHRASSSPGHGPPSKELQFGRGGGSQD
                     SSAETPLAGGLPRLAESPF"
     sig_peptide     950..1024
                     /label=GCGR
     exon            1010..1112
                     /label=GCGR
     mat_peptide     1025..2380
                     /label=glucagon receptor
     STS             1041..1155
                     /label=GCGR
     exon            1113..1220
                     /label=GCGR
     exon            1221..1342
                     /label=GCGR
     exon            1343..1449
                     /label=GCGR
     misc_feature    1376..1447
                     /label=GCGR
                     /note="propagated from UniProtKB/Swiss-Prot (P47871.1); 
                     transmembrane region"
     exon            1450..1606
                     /label=GCGR
     misc_feature    1469..1528
                     /label=GCGR
                     /note="propagated from UniProtKB/Swiss-Prot (P47871.1); 
                     transmembrane region"
     exon            1607..1766
                     /label=GCGR
     misc_feature    1625..1696
                     /label=GCGR
                     /note="propagated from UniProtKB/Swiss-Prot (P47871.1); 
                     transmembrane region"
     misc_feature    1739..1804
                     /label=GCGR
                     /note="propagated from UniProtKB/Swiss-Prot (P47871.1); 
                     transmembrane region"
     exon            1767..1827
                     /label=GCGR
     exon            1828..1897
                     /label=GCGR
     misc_feature    1859..1927
                     /label=GCGR
                     /note="propagated from UniProtKB/Swiss-Prot (P47871.1); 
                     transmembrane region"
     exon            1898..1986
                     /label=GCGR
     exon            1987..2125
                     /label=GCGR
     misc_feature    2000..2056
                     /label=GCGR
                     /note="propagated from UniProtKB/Swiss-Prot (P47871.1); 
                     transmembrane region"
     misc_feature    2093..2161
                     /label=GCGR
                     /note="propagated from UniProtKB/Swiss-Prot (P47871.1); 
                     transmembrane region"
     exon            2126..2167
                     /label=GCGR
     protein_bind    complement(2382..2406)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    2392..2410
                     /label=rfB
                     /note="rfB"
     CDS             2414..2443
                     /codon_start=1
                     /label=GS linker
                     /note="GS linker"
                     /translation="GGGGSGGGGS"
     CDS             2414..2443
                     /codon_start=1
                     /label=GS linker
                     /note="GS linker"
                     /translation="GGGGSGGGGS"
     CDS             2444..2797
                     /codon_start=1
                     /label=TEV1-118
                     /note="TEV1-118"
                     /translation="GESLFKGPRDYNPISSTICHLTNESDGHTTSLYGIGFGPFIITNK
                     HLFRRNNGTLLVQSLHGVFKVKDTTTLQQHLIDGRDMMIIRMPKDFPPFPQKLKFREPQ
                     REERICLVTTNFQA"
     misc_binding    complement(2677..2699)
                     /label=NTEV 5' sequencing primer
                     /note="NTEV 5' sequencing primer"
     CDS             2801..2821
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             2828..2857
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             2858..3754
                     /codon_start=1
                     /label=Gal4-VP16
                     /note="Gal4-VP16"
                     /translation="ARAMKLLSSIEQACDICRLKKLKCSKEKPKCAKCLKNNWECRYSP
                     KTKRSPLTRAHLTEVESRLERLEQLFLLIFPREDLDMILKMDSLQDIKALLTGLFVQDN
                     VNKDAVTDRLASVETDMPLTLRQHRISATSSSEESSNKGQRQLTVSPEFPGISLIIRFT
                     MPKKKRKVAYSRARTKNNYGSTIEGLLDLPDDDAPEEAGLAAPRLSFLPAGHTRRLSTA
                     PPTDVSLGDELHLDGEDVAMAHADALDDFDLDMLGDGDSPGPGFTPHDSAPYGALDMAD
                     FEFEQMFTDALGIDEYGG"
     misc_structure  2858..3310
                     /label=Gal4-DBD core ORF
                     /note="Gal4-DBD core ORF"
     CDS             2867..3307
                     /codon_start=1
                     /gene="Saccharomyces cerevisiae GAL4 (truncated)"
                     /product="DNA binding domain of the GAL4 transcriptional 
                     activator"
                     /label=GAL4 DNA binding domain
                     /translation="MKLLSSIEQACDICRLKKLKCSKEKPKCAKCLKNNWECRYSPKTK
                     RSPLTRAHLTEVESRLERLEQLFLLIFPREDLDMILKMDSLQDIKALLTGLFVQDNVNK
                     DAVTDRLASVETDMPLTLRQHRISATSSSEESSNKGQRQLTVS"
     misc_structure  3350..3754
                     /label=NLS-VP16
                     /note="NLS-VP16"
     CDS             3350..3370
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             3521..3754
                     /codon_start=1
                     /gene="UL48"
                     /product="transcriptional activation domain of herpes 
                     simplex virus protein VP16 (Triezenberg et al., 1988; 
                     Cousens et al., 1989)"
                     /label=VP16 AD
                     /translation="APPTDVSLGDELHLDGEDVAMAHADALDDFDLDMLGDGDSPGPGF
                     TPHDSAPYGALDMADFEFEQMFTDALGIDEYGG"
     CDS             3755..3814
                     /codon_start=1
                     /label=2xHA-tag
                     /note="2xHA-tag"
                     /translation="GYPYDVPDYAGYPYDVPDYA"
     CDS             3758..3784
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             3788..3814
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     promoter        complement(3827..3845)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     3865..3882
                     /label=BGH Reverse priming site
                     /note="BGH Reverse priming site"
     polyA_signal    3871..4095
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      4141..4569
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     promoter        4583..4913
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      4764..4899
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             4980..5774
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     polyA_signal    5948..6069
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6118..6134)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    6142..6158
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(6166..6196)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6211..6232
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     rep_origin      complement(6520..7108)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(7279..8139)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(8140..8244)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggac tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gcttggtacc
      901 gagctcggat cccacatcaa caagtttgta caaaaaagca ggctccacca tgcccccctg
      961 ccagccacag cgacccctgc tgctgttgct gctgctgctg gcctgccagc cacaggtccc
     1021 ctccgctcag gtgatggact tcctgtttga gaagtggaag ctctacggtg accagtgtca
     1081 ccacaacctg agcctgctgc cccctcccac ggagctggtg tgcaacagaa ccttcgacaa
     1141 gtattcctgc tggccggaca cccccgccaa taccacggcc aacatctcct gcccctggta
     1201 cctgccttgg caccacaaag tgcaacaccg cttcgtgttc aagagatgcg ggcccgacgg
     1261 tcagtgggtg cgtggacccc gggggcagcc ttggcgtgat gcctcccagt gccagatgga
     1321 tggcgaggag attgaggtcc agaaggaggt ggccaagatg tacagcagct tccaggtgat
     1381 gtacacagtg ggctacagcc tgtccctggg ggccctgctc ctcgccttgg ccatcctggg
     1441 gggcctcagc aagctgcact gcacccgcaa tgccatccac gcgaatctgt ttgcgtcctt
     1501 cgtgctgaaa gccagctccg tgctggtcat tgatgggctg ctcaggaccc gctacagcca
     1561 gaaaattggc gacgacctca gtgtcagcac ctggctcagt gatggagcgg tggctggctg
     1621 ccgtgtggcc gcggtgttca tgcaatatgg catcgtggcc aactactgct ggctgctggt
     1681 ggagggcctg tacctgcaca acctgctggg cctggccacc ctccccgaga ggagcttctt
     1741 cagcctctac ctgggcatcg gctggggtgc ccccatgctg ttcgtcgtcc cctgggcagt
     1801 ggtcaagtgt ctgttcgaga acgtccagtg ctggaccagc aatgacaaca tgggcttctg
     1861 gtggatcctg cggttccccg tcttcctggc catcctgatc aacttcttca tcttcgtccg
     1921 catcgttcag ctgctcgtgg ccaagctgcg ggcacggcag atgcaccaca cagactacaa
     1981 gttccggctg gccaagtcca cgctgaccct catccctctg ctgggcgtcc acgaagtggt
     2041 cttcgccttc gtgacggacg agcacgccca gggcaccctg cgctccgcca agctcttctt
     2101 cgacctcttc ctcagctcct tccagggcct gctggtggct gtcctctact gcttcctcaa
     2161 caaggaggtg cagtcggagc tgcggcggcg ttggcaccgc tggcgcctgg gcaaagtgct
     2221 atgggaggag cggaacacca gcaaccacag ggcctcatct tcgcccggcc acggccctcc
     2281 cagcaaggag ctgcagtttg ggaggggtgg tggcagccag gattcatctg cggagacccc
     2341 cttggctggt ggcctcccta gattggctga gagccccttc gacccagctt tcttgtacaa
     2401 agtggttgat gtcggaggtg gaggtagcgg aggtggaggt agcggagaga gcttgtttaa
     2461 gggaccacgt gattacaacc cgatatcgag caccatttgt catttgacga atgaatctga
     2521 tgggcacaca acatcgttgt atggtattgg atttggtccc ttcatcatta caaacaagca
     2581 cttgtttcgt cgcaataatg gaacactgtt ggtccaatca ctacatggtg tattcaaggt
     2641 caaggacacc acgactttgc aacaacacct cattgatggg agggacatga tgattattcg
     2701 catgcctaag gatttcccac catttcctca aaagctgaaa tttagagagc cacaaaggga
     2761 agagcgcata tgtcttgtga caaccaactt ccaagctccc gagaacctgt acttccaggg
     2821 ttctagagaa cagaagctga tctcagaaga ggatctggcc cgggccatga agctactgtc
     2881 ttctatcgaa caagcatgcg atatttgccg acttaaaaag ctcaagtgct ccaaagaaaa
     2941 accgaagtgc gccaagtgtc tgaagaacaa ctgggagtgt cgctactctc ccaaaaccaa
     3001 aaggtctccg ctgactaggg cacatctgac agaagtggaa tcaaggctag aaagactgga
     3061 acagctattt ctactgattt ttcctcgaga agaccttgac atgattttga aaatggattc
     3121 tttacaggat ataaaagcat tgttaacagg attatttgta caagataatg tgaataaaga
     3181 tgccgtcaca gatagattgg cttcagtgga gactgatatg cctctaacat tgagacagca
     3241 tagaataagt gcgacatcat catcggaaga gagtagtaac aaaggtcaaa gacagttgac
     3301 tgtatcgccg gaattcccgg ggatcagctt gataattcga ttcaccatgc ccaagaagaa
     3361 gaggaaggtg gcgtacagcc gcgcgcgtac gaaaaacaat tacgggtcta ccatcgaggg
     3421 cctgctcgat ctcccggacg acgacgcccc cgaagaggcg gggctggcgg ctccgcgcct
     3481 gtcctttctc cccgcgggac acacgcgcag actgtcgacg gcccccccga ccgatgtcag
     3541 cctgggggac gagctccact tagacggcga ggacgtcgcg atggcgcatg ccgacgcgct
     3601 agacgatttc gatctggaca tgttggggga cggggattcc ccgggtccgg gatttacccc
     3661 ccacgactcc gccccctacg gcgctctgga tatggccgac ttcgagtttg agcagatgtt
     3721 taccgatgcc cttggaattg acgagtacgg tgggggctac ccatacgatg ttcctgacta
     3781 tgcgggctat ccctatgacg tcccggacta tgcatgaggg ccctattcta tagtgtcacc
     3841 taaatgctag agctcgctga tcagcctcga ctgtgccttc tagttgccag ccatctgttg
     3901 tttgcccctc ccccgtgcct tccttgaccc tggaaggtgc cactcccact gtcctttcct
     3961 aataaaatga ggaaattgca tcgcattgtc tgagtaggtg tcattctatt ctggggggtg
     4021 gggtggggca ggacagcaag ggggaggatt gggaagacaa tagcaggcat gctggggatg
     4081 cggtgggctc tatggcttct gaggcggaaa gaaccagctg gggctctagg gggtatcccc
     4141 acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg
     4201 ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca
     4261 cgttcgccgg ctttccccgt caagctctaa atcggggcat ccctttaggg ttccgattta
     4321 gtgctttacg gcacctcgac cccaaaaaac ttgattaggg tgatggttca cgtagtgggc
     4381 catcgccctg atagacggtt tttcgccctt tgacgttgga gtccacgttc tttaatagtg
     4441 gactcttgtt ccaaactgga acaacactca accctatctc ggtctattct tttgatttat
     4501 aagggatttt ggggatttcg gcctattggt taaaaaatga gctgatttaa caaaaattta
     4561 acgcgaatta attctgtgga atgtgtgtca gttagggtgt ggaaagtccc caggctcccc
     4621 aggcaggcag aagtatgcaa agcatgcatc tcaattagtc agcaaccagg tgtggaaagt
     4681 ccccaggctc cccagcaggc agaagtatgc aaagcatgca tctcaattag tcagcaacca
     4741 tagtcccgcc cctaactccg cccatcccgc ccctaactcc gcccagttcc gcccattctc
     4801 cgccccatgg ctgactaatt ttttttattt atgcagaggc cgaggccgcc tctgcctctg
     4861 agctattcca gaagtagtga ggaggctttt ttggaggcct aggcttttgc aaaaagctcc
     4921 cgggagcttg tatatccatt ttcggatctg atcaagagac aggatgagga tcgtttcgca
     4981 tgattgaaca agatggattg cacgcaggtt ctccggccgc ttgggtggag aggctattcg
     5041 gctatgactg ggcacaacag acaatcggct gctctgatgc cgccgtgttc cggctgtcag
     5101 cgcaggggcg cccggttctt tttgtcaaga ccgacctgtc cggtgccctg aatgaactgc
     5161 aggacgaggc agcgcggcta tcgtggctgg ccacgacggg cgttccttgc gcagctgtgc
     5221 tcgacgttgt cactgaagcg ggaagggact ggctgctatt gggcgaagtg ccggggcagg
     5281 atctcctgtc atctcacctt gctcctgccg agaaagtatc catcatggct gatgcaatgc
     5341 ggcggctgca tacgcttgat ccggctacct gcccattcga ccaccaagcg aaacatcgca
     5401 tcgagcgagc acgtactcgg atggaagccg gtcttgtcga tcaggatgat ctggacgaag
     5461 agcatcaggg gctcgcgcca gccgaactgt tcgccaggct caaggcgcgc atgcccgacg
     5521 gcgaggatct cgtcgtgacc catggcgatg cctgcttgcc gaatatcatg gtggaaaatg
     5581 gccgcttttc tggattcatc gactgtggcc ggctgggtgt ggcggaccgc tatcaggaca
     5641 tagcgttggc tacccgtgat attgctgaag agcttggcgg cgaatgggct gaccgcttcc
     5701 tcgtgcttta cggtatcgcc gctcccgatt cgcagcgcat cgccttctat cgccttcttg
     5761 acgagttctt ctgagcggga ctctggggtt cgaaatgacc gaccaagcga cgcccaacct
     5821 gccatcacga gatttcgatt ccaccgccgc cttctatgaa aggttgggct tcggaatcgt
     5881 tttccgggac gccggctgga tgatcctcca gcgcggggat ctcatgctgg agttcttcgc
     5941 ccaccccaac ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa
     6001 tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa
     6061 tgtatcttat catgtctgta taccgtcgac ctctagctag agcttggcgt aatcatggtc
     6121 atagctgttt cctgtgtgaa attgttatcc gctcacaatt ccacacaaca tacgagccgg
     6181 aagcataaag tgtaaagcct ggggtgccta atgagtgagc taactcacat taattgcgtt
     6241 gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg
     6301 ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct tccgcttcct cgctcactga
     6361 ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat
     6421 acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca
     6481 aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc
     6541 tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata
     6601 aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc
     6661 gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcaatgctc
     6721 acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga
     6781 accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc
     6841 ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag
     6901 gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag
     6961 gacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag
     7021 ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca
     7081 gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga
     7141 cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat
     7201 cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga
     7261 gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg
     7321 tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga
     7381 gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc
     7441 agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac
     7501 tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc
     7561 agttaatagt ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc
     7621 gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc
     7681 catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt
     7741 ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc
     7801 atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg
     7861 tatgcggcga ccgagttgct cttgcccggc gtcaatacgg gataataccg cgccacatag
     7921 cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat
     7981 cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc
     8041 atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa
     8101 aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta
     8161 ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa
     8221 aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccacctg acgtc
//
