LOCUS       Exported                8356 bp ds-DNA     circular SYN 11-NOV-2022
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8356)
  AUTHORS   Michael Wehr
  TITLE     Direct Submission
  JOURNAL   Exported Monday, Nov 14, 2022 from SnapGene 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..8356
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     enhancer        235..614
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        615..818
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     863..882
                     /label=T7 promoter/priming site
                     /note="T7 promoter/priming site"
     promoter        863..881
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     misc_feature    916..927
                     /label=rfB
                     /note="rfB"
     protein_bind    920..944
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    945..1030
                     /label=TANGO Presto 5' signal peptide
     CDS             950..997
                     /codon_start=1
                     /label=HA1 signal peptide
                     /translation="MKTIIALSYIFCLVFA"
     CDS             1031..2461
                     /codon_start=1
                     /label=GCGR Cop, NM_000160
                     /note="GL-R"
                     /translation="MPPCQPQRPLLLLLLLLACQPQVPSAQVMDFLFEKWKLYGDQCHH
                     NLSLLPPPTELVCNRTFDKYSCWPDTPANTTANISCPWYLPWHHKVQHRFVFKRCGPDG
                     QWVRGPRGQPWRDASQCQMDGEEIEVQKEVAKMYSSFQVMYTVGYSLSLGALLLALAIL
                     GGLSKLHCTRNAIHANLFASFVLKASSVLVIDGLLRTRYSQKIGDDLSVSTWLSDGAVA
                     GCRVAAVFMQYGIVANYCWLLVEGLYLHNLLGLATLPERSFFSLYLGIGWGAPMLFVVP
                     WAVVKCLFENVQCWTSNDNMGFWWILRFPVFLAILINFFIFVRIVQLLVAKLRARQMHH
                     TDYKFRLAKSTLTLIPLLGVHEVVFAFVTDEHAQGTLRSAKLFFDLFLSSFQGLLVAVL
                     YCFLNKEVQSELRRRWHRWRLGKVLWEERNTSNHRASSSPGHGPPSKELQFGRGGGSQD
                     SSAETPLAGGLPRLAESPF"
     sig_peptide     1031..1105
                     /label=GCGR
     exon            1091..1193
                     /label=GCGR
     mat_peptide     1106..2461
                     /label=glucagon receptor
     STS             1122..1236
                     /label=GCGR
     exon            1194..1301
                     /label=GCGR
     exon            1302..1423
                     /label=GCGR
     exon            1424..1530
                     /label=GCGR
     misc_feature    1457..1528
                     /label=GCGR
                     /note="propagated from UniProtKB/Swiss-Prot (P47871.1); 
                     transmembrane region"
     exon            1531..1687
                     /label=GCGR
     misc_feature    1550..1609
                     /label=GCGR
                     /note="propagated from UniProtKB/Swiss-Prot (P47871.1); 
                     transmembrane region"
     exon            1688..1847
                     /label=GCGR
     misc_feature    1706..1777
                     /label=GCGR
                     /note="propagated from UniProtKB/Swiss-Prot (P47871.1); 
                     transmembrane region"
     misc_feature    1820..1885
                     /label=GCGR
                     /note="propagated from UniProtKB/Swiss-Prot (P47871.1); 
                     transmembrane region"
     exon            1848..1908
                     /label=GCGR
     exon            1909..1978
                     /label=GCGR
     misc_feature    1940..2008
                     /label=GCGR
                     /note="propagated from UniProtKB/Swiss-Prot (P47871.1); 
                     transmembrane region"
     exon            1979..2067
                     /label=GCGR
     exon            2068..2206
                     /label=GCGR
     misc_feature    2081..2137
                     /label=GCGR
                     /note="propagated from UniProtKB/Swiss-Prot (P47871.1); 
                     transmembrane region"
     misc_feature    2174..2242
                     /label=GCGR
                     /note="propagated from UniProtKB/Swiss-Prot (P47871.1); 
                     transmembrane region"
     exon            2207..2248
                     /label=GCGR
     protein_bind    complement(2463..2487)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    2473..2491
                     /label=rfB
                     /note="rfB"
     CDS             2495..2524
                     /codon_start=1
                     /label=GS linker
                     /note="GS linker"
                     /translation="GGGGSGGGGS"
     CDS             2495..2524
                     /codon_start=1
                     /label=GS linker
                     /note="GS linker"
                     /translation="GGGGSGGGGS"
     CDS             2525..2878
                     /codon_start=1
                     /label=TEV1-118
                     /note="TEV1-118"
                     /translation="GESLFKGPRDYNPISSTICHLTNESDGHTTSLYGIGFGPFIITNK
                     HLFRRNNGTLLVQSLHGVFKVKDTTTLQQHLIDGRDMMIIRMPKDFPPFPQKLKFREPQ
                     REERICLVTTNFQA"
     misc_binding    complement(2758..2780)
                     /label=NTEV 5' sequencing primer
                     /note="NTEV 5' sequencing primer"
     CDS             2882..2902
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             2909..2938
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             2939..3835
                     /codon_start=1
                     /label=Gal4-VP16
                     /note="Gal4-VP16"
                     /translation="ARAMKLLSSIEQACDICRLKKLKCSKEKPKCAKCLKNNWECRYSP
                     KTKRSPLTRAHLTEVESRLERLEQLFLLIFPREDLDMILKMDSLQDIKALLTGLFVQDN
                     VNKDAVTDRLASVETDMPLTLRQHRISATSSSEESSNKGQRQLTVSPEFPGISLIIRFT
                     MPKKKRKVAYSRARTKNNYGSTIEGLLDLPDDDAPEEAGLAAPRLSFLPAGHTRRLSTA
                     PPTDVSLGDELHLDGEDVAMAHADALDDFDLDMLGDGDSPGPGFTPHDSAPYGALDMAD
                     FEFEQMFTDALGIDEYGG"
     misc_structure  2939..3391
                     /label=Gal4-DBD core ORF
                     /note="Gal4-DBD core ORF"
     CDS             2948..3388
                     /codon_start=1
                     /gene="Saccharomyces cerevisiae GAL4 (truncated)"
                     /product="DNA binding domain of the GAL4 transcriptional 
                     activator"
                     /label=GAL4 DNA binding domain
                     /translation="MKLLSSIEQACDICRLKKLKCSKEKPKCAKCLKNNWECRYSPKTK
                     RSPLTRAHLTEVESRLERLEQLFLLIFPREDLDMILKMDSLQDIKALLTGLFVQDNVNK
                     DAVTDRLASVETDMPLTLRQHRISATSSSEESSNKGQRQLTVS"
     misc_structure  3431..3835
                     /label=NLS-VP16
                     /note="NLS-VP16"
     CDS             3431..3451
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             3602..3835
                     /codon_start=1
                     /gene="UL48"
                     /product="transcriptional activation domain of herpes 
                     simplex virus protein VP16 (Triezenberg et al., 1988; 
                     Cousens et al., 1989)"
                     /label=VP16 AD
                     /translation="APPTDVSLGDELHLDGEDVAMAHADALDDFDLDMLGDGDSPGPGF
                     TPHDSAPYGALDMADFEFEQMFTDALGIDEYGG"
     CDS             3836..3895
                     /codon_start=1
                     /label=2xHA-tag
                     /note="2xHA-tag"
                     /translation="GYPYDVPDYAGYPYDVPDYA"
     CDS             3839..3865
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             3869..3895
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     promoter        complement(3908..3926)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     3946..3963
                     /label=BGH Reverse priming site
                     /note="BGH Reverse priming site"
     polyA_signal    3952..4176
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      4222..4650
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     promoter        4664..4994
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      4845..4980
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             5061..5855
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     polyA_signal    6029..6150
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6199..6215)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    6223..6239
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(6247..6277)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6292..6313
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     rep_origin      complement(6601..7189)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(7360..8220)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(8221..8325)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggac tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gcttggtacc
      901 gagctcggat cccacatcaa caagtttgta caaaaaagca ggctccacca tgaagacgat
      961 catcgccctg agctacatct tctgcctggt attcgccgac tacaaggacg atgatgacgc
     1021 cagcatcgat atgcccccct gccagccaca gcgacccctg ctgctgttgc tgctgctgct
     1081 ggcctgccag ccacaggtcc cctccgctca ggtgatggac ttcctgtttg agaagtggaa
     1141 gctctacggt gaccagtgtc accacaacct gagcctgctg ccccctccca cggagctggt
     1201 gtgcaacaga accttcgaca agtattcctg ctggccggac acccccgcca ataccacggc
     1261 caacatctcc tgcccctggt acctgccttg gcaccacaaa gtgcaacacc gcttcgtgtt
     1321 caagagatgc gggcccgacg gtcagtgggt gcgtggaccc cgggggcagc cttggcgtga
     1381 tgcctcccag tgccagatgg atggcgagga gattgaggtc cagaaggagg tggccaagat
     1441 gtacagcagc ttccaggtga tgtacacagt gggctacagc ctgtccctgg gggccctgct
     1501 cctcgccttg gccatcctgg ggggcctcag caagctgcac tgcacccgca atgccatcca
     1561 cgcgaatctg tttgcgtcct tcgtgctgaa agccagctcc gtgctggtca ttgatgggct
     1621 gctcaggacc cgctacagcc agaaaattgg cgacgacctc agtgtcagca cctggctcag
     1681 tgatggagcg gtggctggct gccgtgtggc cgcggtgttc atgcaatatg gcatcgtggc
     1741 caactactgc tggctgctgg tggagggcct gtacctgcac aacctgctgg gcctggccac
     1801 cctccccgag aggagcttct tcagcctcta cctgggcatc ggctggggtg cccccatgct
     1861 gttcgtcgtc ccctgggcag tggtcaagtg tctgttcgag aacgtccagt gctggaccag
     1921 caatgacaac atgggcttct ggtggatcct gcggttcccc gtcttcctgg ccatcctgat
     1981 caacttcttc atcttcgtcc gcatcgttca gctgctcgtg gccaagctgc gggcacggca
     2041 gatgcaccac acagactaca agttccggct ggccaagtcc acgctgaccc tcatccctct
     2101 gctgggcgtc cacgaagtgg tcttcgcctt cgtgacggac gagcacgccc agggcaccct
     2161 gcgctccgcc aagctcttct tcgacctctt cctcagctcc ttccagggcc tgctggtggc
     2221 tgtcctctac tgcttcctca acaaggaggt gcagtcggag ctgcggcggc gttggcaccg
     2281 ctggcgcctg ggcaaagtgc tatgggagga gcggaacacc agcaaccaca gggcctcatc
     2341 ttcgcccggc cacggccctc ccagcaagga gctgcagttt gggaggggtg gtggcagcca
     2401 ggattcatct gcggagaccc ccttggctgg tggcctccct agattggctg agagcccctt
     2461 cgacccagct ttcttgtaca aagtggttga tgtcggaggt ggaggtagcg gaggtggagg
     2521 tagcggagag agcttgttta agggaccacg tgattacaac ccgatatcga gcaccatttg
     2581 tcatttgacg aatgaatctg atgggcacac aacatcgttg tatggtattg gatttggtcc
     2641 cttcatcatt acaaacaagc acttgtttcg tcgcaataat ggaacactgt tggtccaatc
     2701 actacatggt gtattcaagg tcaaggacac cacgactttg caacaacacc tcattgatgg
     2761 gagggacatg atgattattc gcatgcctaa ggatttccca ccatttcctc aaaagctgaa
     2821 atttagagag ccacaaaggg aagagcgcat atgtcttgtg acaaccaact tccaagctcc
     2881 cgagaacctg tacttccagg gttctagaga acagaagctg atctcagaag aggatctggc
     2941 ccgggccatg aagctactgt cttctatcga acaagcatgc gatatttgcc gacttaaaaa
     3001 gctcaagtgc tccaaagaaa aaccgaagtg cgccaagtgt ctgaagaaca actgggagtg
     3061 tcgctactct cccaaaacca aaaggtctcc gctgactagg gcacatctga cagaagtgga
     3121 atcaaggcta gaaagactgg aacagctatt tctactgatt tttcctcgag aagaccttga
     3181 catgattttg aaaatggatt ctttacagga tataaaagca ttgttaacag gattatttgt
     3241 acaagataat gtgaataaag atgccgtcac agatagattg gcttcagtgg agactgatat
     3301 gcctctaaca ttgagacagc atagaataag tgcgacatca tcatcggaag agagtagtaa
     3361 caaaggtcaa agacagttga ctgtatcgcc ggaattcccg gggatcagct tgataattcg
     3421 attcaccatg cccaagaaga agaggaaggt ggcgtacagc cgcgcgcgta cgaaaaacaa
     3481 ttacgggtct accatcgagg gcctgctcga tctcccggac gacgacgccc ccgaagaggc
     3541 ggggctggcg gctccgcgcc tgtcctttct ccccgcggga cacacgcgca gactgtcgac
     3601 ggcccccccg accgatgtca gcctggggga cgagctccac ttagacggcg aggacgtcgc
     3661 gatggcgcat gccgacgcgc tagacgattt cgatctggac atgttggggg acggggattc
     3721 cccgggtccg ggatttaccc cccacgactc cgccccctac ggcgctctgg atatggccga
     3781 cttcgagttt gagcagatgt ttaccgatgc ccttggaatt gacgagtacg gtgggggcta
     3841 cccatacgat gttcctgact atgcgggcta tccctatgac gtcccggact atgcatgagg
     3901 gccctattct atagtgtcac ctaaatgcta gagctcgctg atcagcctcg actgtgcctt
     3961 ctagttgcca gccatctgtt gtttgcccct cccccgtgcc ttccttgacc ctggaaggtg
     4021 ccactcccac tgtcctttcc taataaaatg aggaaattgc atcgcattgt ctgagtaggt
     4081 gtcattctat tctggggggt ggggtggggc aggacagcaa gggggaggat tgggaagaca
     4141 atagcaggca tgctggggat gcggtgggct ctatggcttc tgaggcggaa agaaccagct
     4201 ggggctctag ggggtatccc cacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg
     4261 tggttacgcg cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt
     4321 tcttcccttc ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcggggca
     4381 tccctttagg gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgattagg
     4441 gtgatggttc acgtagtggg ccatcgccct gatagacggt ttttcgccct ttgacgttgg
     4501 agtccacgtt ctttaatagt ggactcttgt tccaaactgg aacaacactc aaccctatct
     4561 cggtctattc ttttgattta taagggattt tggggatttc ggcctattgg ttaaaaaatg
     4621 agctgattta acaaaaattt aacgcgaatt aattctgtgg aatgtgtgtc agttagggtg
     4681 tggaaagtcc ccaggctccc caggcaggca gaagtatgca aagcatgcat ctcaattagt
     4741 cagcaaccag gtgtggaaag tccccaggct ccccagcagg cagaagtatg caaagcatgc
     4801 atctcaatta gtcagcaacc atagtcccgc ccctaactcc gcccatcccg cccctaactc
     4861 cgcccagttc cgcccattct ccgccccatg gctgactaat tttttttatt tatgcagagg
     4921 ccgaggccgc ctctgcctct gagctattcc agaagtagtg aggaggcttt tttggaggcc
     4981 taggcttttg caaaaagctc ccgggagctt gtatatccat tttcggatct gatcaagaga
     5041 caggatgagg atcgtttcgc atgattgaac aagatggatt gcacgcaggt tctccggccg
     5101 cttgggtgga gaggctattc ggctatgact gggcacaaca gacaatcggc tgctctgatg
     5161 ccgccgtgtt ccggctgtca gcgcaggggc gcccggttct ttttgtcaag accgacctgt
     5221 ccggtgccct gaatgaactg caggacgagg cagcgcggct atcgtggctg gccacgacgg
     5281 gcgttccttg cgcagctgtg ctcgacgttg tcactgaagc gggaagggac tggctgctat
     5341 tgggcgaagt gccggggcag gatctcctgt catctcacct tgctcctgcc gagaaagtat
     5401 ccatcatggc tgatgcaatg cggcggctgc atacgcttga tccggctacc tgcccattcg
     5461 accaccaagc gaaacatcgc atcgagcgag cacgtactcg gatggaagcc ggtcttgtcg
     5521 atcaggatga tctggacgaa gagcatcagg ggctcgcgcc agccgaactg ttcgccaggc
     5581 tcaaggcgcg catgcccgac ggcgaggatc tcgtcgtgac ccatggcgat gcctgcttgc
     5641 cgaatatcat ggtggaaaat ggccgctttt ctggattcat cgactgtggc cggctgggtg
     5701 tggcggaccg ctatcaggac atagcgttgg ctacccgtga tattgctgaa gagcttggcg
     5761 gcgaatgggc tgaccgcttc ctcgtgcttt acggtatcgc cgctcccgat tcgcagcgca
     5821 tcgccttcta tcgccttctt gacgagttct tctgagcggg actctggggt tcgaaatgac
     5881 cgaccaagcg acgcccaacc tgccatcacg agatttcgat tccaccgccg ccttctatga
     5941 aaggttgggc ttcggaatcg ttttccggga cgccggctgg atgatcctcc agcgcgggga
     6001 tctcatgctg gagttcttcg cccaccccaa cttgtttatt gcagcttata atggttacaa
     6061 ataaagcaat agcatcacaa atttcacaaa taaagcattt ttttcactgc attctagttg
     6121 tggtttgtcc aaactcatca atgtatctta tcatgtctgt ataccgtcga cctctagcta
     6181 gagcttggcg taatcatggt catagctgtt tcctgtgtga aattgttatc cgctcacaat
     6241 tccacacaac atacgagccg gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag
     6301 ctaactcaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg
     6361 ccagctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc
     6421 ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc
     6481 agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa
     6541 catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt
     6601 tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg
     6661 gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg
     6721 ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag
     6781 cgtggcgctt tctcaatgct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc
     6841 caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa
     6901 ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg
     6961 taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc
     7021 taactacggc tacactagaa ggacagtatt tggtatctgc gctctgctga agccagttac
     7081 cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg
     7141 tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt
     7201 gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt
     7261 catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa
     7321 atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga
     7381 ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt
     7441 gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg
     7501 agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga
     7561 gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga
     7621 agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg
     7681 catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc
     7741 aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc
     7801 gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca
     7861 taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac
     7921 caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg
     7981 ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc
     8041 ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg
     8101 tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac
     8161 aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat
     8221 actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata
     8281 catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa
     8341 agtgccacct gacgtc
//
