LOCUS Exported 8350 bp ds-DNA circular SYN 11-NOV-2022 DEFINITION synthetic circular DNA ACCESSION . VERSION . KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 8350) AUTHORS Michael Wehr TITLE Direct Submission JOURNAL Exported Monday, Nov 14, 2022 from SnapGene 5.0.8 https://www.snapgene.com FEATURES Location/Qualifiers source 1..8350 /organism="synthetic DNA construct" /mol_type="other DNA" promoter 209..863 /label=CMV promotor /note="CMV promotor" enhancer 235..614 /label=CMV enhancer /note="human cytomegalovirus immediate early enhancer" misc_feature 440 /label=C->G promoter 615..818 /label=CMV promoter /note="human cytomegalovirus (CMV) immediate early promoter" primer_bind 863..882 /label=T7 promoter/priming site /note="T7 promoter/priming site" promoter 863..881 /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" misc_feature 895..906 /gene="miscellaneous" /label=reading Frame B /note="reading Frame B" /note="/SECDrawas=Region" protein_bind 899..923 /gene="mutant version of attB" /label=attB1 /bound_moiety="BP Clonase(TM)" /note="recombination site for the Gateway(R) BP reaction" CDS 929..2359 /codon_start=1 /label=GCGR Cop, NM_000160 /note="GL-R" /translation="MPPCQPQRPLLLLLLLLACQPQVPSAQVMDFLFEKWKLYGDQCHH NLSLLPPPTELVCNRTFDKYSCWPDTPANTTANISCPWYLPWHHKVQHRFVFKRCGPDG QWVRGPRGQPWRDASQCQMDGEEIEVQKEVAKMYSSFQVMYTVGYSLSLGALLLALAIL GGLSKLHCTRNAIHANLFASFVLKASSVLVIDGLLRTRYSQKIGDDLSVSTWLSDGAVA GCRVAAVFMQYGIVANYCWLLVEGLYLHNLLGLATLPERSFFSLYLGIGWGAPMLFVVP WAVVKCLFENVQCWTSNDNMGFWWILRFPVFLAILINFFIFVRIVQLLVAKLRARQMHH TDYKFRLAKSTLTLIPLLGVHEVVFAFVTDEHAQGTLRSAKLFFDLFLSSFQGLLVAVL YCFLNKEVQSELRRRWHRWRLGKVLWEERNTSNHRASSSPGHGPPSKELQFGRGGGSQD SSAETPLAGGLPRLAESPF" sig_peptide 929..1003 /label=GCGR exon 989..1091 /label=GCGR mat_peptide 1004..2359 /label=glucagon receptor STS 1020..1134 /label=GCGR exon 1092..1199 /label=GCGR exon 1200..1321 /label=GCGR exon 1322..1428 /label=GCGR misc_feature 1355..1426 /label=GCGR /note="propagated from UniProtKB/Swiss-Prot (P47871.1); transmembrane region" exon 1429..1585 /label=GCGR misc_feature 1448..1507 /label=GCGR /note="propagated from UniProtKB/Swiss-Prot (P47871.1); transmembrane region" exon 1586..1745 /label=GCGR misc_feature 1604..1675 /label=GCGR /note="propagated from UniProtKB/Swiss-Prot (P47871.1); transmembrane region" misc_feature 1718..1783 /label=GCGR /note="propagated from UniProtKB/Swiss-Prot (P47871.1); transmembrane region" exon 1746..1806 /label=GCGR exon 1807..1876 /label=GCGR misc_feature 1838..1906 /label=GCGR /note="propagated from UniProtKB/Swiss-Prot (P47871.1); transmembrane region" exon 1877..1965 /label=GCGR exon 1966..2104 /label=GCGR misc_feature 1979..2035 /label=GCGR /note="propagated from UniProtKB/Swiss-Prot (P47871.1); transmembrane region" misc_feature 2072..2140 /label=GCGR /note="propagated from UniProtKB/Swiss-Prot (P47871.1); transmembrane region" exon 2105..2146 /label=GCGR protein_bind complement(2361..2385) /gene="mutant version of attB" /label=attB2 /bound_moiety="BP Clonase(TM)" /note="recombination site for the Gateway(R) BP reaction" misc_feature 2371..2389 /gene="miscellaneous" /label=reading Frame B /note="reading Frame B" /note="/SECDrawas=Region" CDS 2396..2482 /codon_start=1 /gene="coding sequence" /label=V2R aa343-371 /note="V2R aa343-371" /note="/SECDrawas=Region" /translation="ARGRTPPSLGPQDESCTTASSSLAKDTSS" CDS 2489..2518 /codon_start=1 /label=GS linker /note="GS linker" /translation="GGGGSGGGGS" CDS 2519..2872 /codon_start=1 /label=TEV1-118 /note="TEV1-118" /translation="GESLFKGPRDYNPISSTICHLTNESDGHTTSLYGIGFGPFIITNK HLFRRNNGTLLVQSLHGVFKVKDTTTLQQHLIDGRDMMIIRMPKDFPPFPQKLKFREPQ REERICLVTTNFQA" misc_binding complement(2752..2774) /label=NTEV 5' sequencing primer /note="NTEV 5' sequencing primer" CDS 2876..2896 /codon_start=1 /label=tevS /note="tevS (TEV cleavage site)" /translation="ENLYFQG" CDS 2876..2896 /codon_start=1 /product="tobacco etch virus (TEV) protease recognition and cleavage site" /label=TEV site /translation="ENLYFQG" CDS 2903..2932 /codon_start=1 /label=Myc-tag /note="Myc-tag (due to cloning reasons - taken from old pDEST_X-ASPlink-NTEV-tevS-GV which contained that Myc-tag already)" /translation="EQKLISEEDL" CDS 2903..2932 /codon_start=1 /product="Myc (human c-Myc proto-oncogene) epitope tag" /label=Myc /translation="EQKLISEEDL" CDS 2933..3829 /codon_start=1 /label=Gal4-VP16 /note="Gal4-VP16" /translation="ARAMKLLSSIEQACDICRLKKLKCSKEKPKCAKCLKNNWECRYSP KTKRSPLTRAHLTEVESRLERLEQLFLLIFPREDLDMILKMDSLQDIKALLTGLFVQDN VNKDAVTDRLASVETDMPLTLRQHRISATSSSEESSNKGQRQLTVSPEFPGISLIIRFT MPKKKRKVAYSRARTKNNYGSTIEGLLDLPDDDAPEEAGLAAPRLSFLPAGHTRRLSTA PPTDVSLGDELHLDGEDVAMAHADALDDFDLDMLGDGDSPGPGFTPHDSAPYGALDMAD FEFEQMFTDALGIDEYGG" misc_structure 2933..3385 /label=Gal4-DBD core ORF /note="Gal4-DBD core ORF" CDS 2942..3382 /codon_start=1 /gene="Saccharomyces cerevisiae GAL4 (truncated)" /product="DNA binding domain of the GAL4 transcriptional activator" /label=GAL4 DNA binding domain /translation="MKLLSSIEQACDICRLKKLKCSKEKPKCAKCLKNNWECRYSPKTK RSPLTRAHLTEVESRLERLEQLFLLIFPREDLDMILKMDSLQDIKALLTGLFVQDNVNK DAVTDRLASVETDMPLTLRQHRISATSSSEESSNKGQRQLTVS" misc_structure 3425..3829 /label=NLS-VP16 /note="NLS-VP16" CDS 3425..3445 /codon_start=1 /product="nuclear localization signal of SV40 (simian virus 40) large T antigen" /label=SV40 NLS /translation="PKKKRKV" CDS 3596..3829 /codon_start=1 /gene="UL48" /product="transcriptional activation domain of herpes simplex virus protein VP16 (Triezenberg et al., 1988; Cousens et al., 1989)" /label=VP16 AD /translation="APPTDVSLGDELHLDGEDVAMAHADALDDFDLDMLGDGDSPGPGF TPHDSAPYGALDMADFEFEQMFTDALGIDEYGG" CDS 3830..3889 /codon_start=1 /label=2xHA-tag /note="2xHA-tag" /translation="GYPYDVPDYAGYPYDVPDYA" CDS 3833..3859 /codon_start=1 /product="HA (human influenza hemagglutinin) epitope tag" /label=HA /translation="YPYDVPDYA" CDS 3863..3889 /codon_start=1 /product="HA (human influenza hemagglutinin) epitope tag" /label=HA /translation="YPYDVPDYA" promoter complement(3902..3920) /label=SP6 promoter /note="promoter for bacteriophage SP6 RNA polymerase" polyA_site 3922..4153 /label=BGH polyA /note="BGH polyA" primer_bind 3940..3957 /label=BGH Reverse priming site /note="BGH Reverse priming site" polyA_signal 3946..4170 /label=bGH poly(A) signal /note="bovine growth hormone polyadenylation signal" rep_origin 4216..4644 /direction=RIGHT /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" promoter 4658..4988 /label=SV40 promoter /note="SV40 enhancer and early promoter" rep_origin 4839..4974 /label=SV40 ori /note="SV40 origin of replication" CDS 5055..5849 /codon_start=1 /gene="aph(3')-II (or nptII)" /product="aminoglycoside phosphotransferase from Tn5" /label=NeoR/KanR /note="confers resistance to neomycin, kanamycin, and G418 (Geneticin(R))" /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF" polyA_signal 5904..6276 /label=SV40 poly A /note="SV40 poly A" polyA_signal 6023..6144 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" primer_bind complement(6193..6209) /label=M13 rev /note="common sequencing primer, one of multiple similar variants" protein_bind 6217..6233 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." promoter complement(6241..6271) /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 6286..6307 /label=CAP binding site /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." rep_origin complement(6536..7209) /direction=LEFT /label=ColE1 origin (pBR3222 origin) /note="ColE1 origin (pBR3222 origin)" rep_origin complement(6595..7183) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" CDS complement(7354..8214) /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" promoter complement(8215..8319) /gene="bla" /label=AmpR promoter ORIGIN 1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg 61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg 121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc 181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt 241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata 301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc 361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc 421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt 481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt 541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca 601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg 661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc 721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg 781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca 841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gcttatcaac 901 aagtttgtac aaaaaagcag gctccaccat gcccccctgc cagccacagc gacccctgct 961 gctgttgctg ctgctgctgg cctgccagcc acaggtcccc tccgctcagg tgatggactt 1021 cctgtttgag aagtggaagc tctacggtga ccagtgtcac cacaacctga gcctgctgcc 1081 ccctcccacg gagctggtgt gcaacagaac cttcgacaag tattcctgct ggccggacac 1141 ccccgccaat accacggcca acatctcctg cccctggtac ctgccttggc accacaaagt 1201 gcaacaccgc ttcgtgttca agagatgcgg gcccgacggt cagtgggtgc gtggaccccg 1261 ggggcagcct tggcgtgatg cctcccagtg ccagatggat ggcgaggaga ttgaggtcca 1321 gaaggaggtg gccaagatgt acagcagctt ccaggtgatg tacacagtgg gctacagcct 1381 gtccctgggg gccctgctcc tcgccttggc catcctgggg ggcctcagca agctgcactg 1441 cacccgcaat gccatccacg cgaatctgtt tgcgtccttc gtgctgaaag ccagctccgt 1501 gctggtcatt gatgggctgc tcaggacccg ctacagccag aaaattggcg acgacctcag 1561 tgtcagcacc tggctcagtg atggagcggt ggctggctgc cgtgtggccg cggtgttcat 1621 gcaatatggc atcgtggcca actactgctg gctgctggtg gagggcctgt acctgcacaa 1681 cctgctgggc ctggccaccc tccccgagag gagcttcttc agcctctacc tgggcatcgg 1741 ctggggtgcc cccatgctgt tcgtcgtccc ctgggcagtg gtcaagtgtc tgttcgagaa 1801 cgtccagtgc tggaccagca atgacaacat gggcttctgg tggatcctgc ggttccccgt 1861 cttcctggcc atcctgatca acttcttcat cttcgtccgc atcgttcagc tgctcgtggc 1921 caagctgcgg gcacggcaga tgcaccacac agactacaag ttccggctgg ccaagtccac 1981 gctgaccctc atccctctgc tgggcgtcca cgaagtggtc ttcgccttcg tgacggacga 2041 gcacgcccag ggcaccctgc gctccgccaa gctcttcttc gacctcttcc tcagctcctt 2101 ccagggcctg ctggtggctg tcctctactg cttcctcaac aaggaggtgc agtcggagct 2161 gcggcggcgt tggcaccgct ggcgcctggg caaagtgcta tgggaggagc ggaacaccag 2221 caaccacagg gcctcatctt cgcccggcca cggccctccc agcaaggagc tgcagtttgg 2281 gaggggtggt ggcagccagg attcatctgc ggagaccccc ttggctggtg gcctccctag 2341 attggctgag agccccttcg acccagcttt cttgtacaaa gtggttgatg gtaccgcccg 2401 gggacgcacc ccacccagcc tgggtcccca agatgagtcc tgcaccaccg ccagctcctc 2461 cctggccaag gacacttcat cggatgtcgg aggtggaggt agcggaggtg gaggtagcgg 2521 agagagcttg tttaagggac cacgtgatta caacccgata tcgagcacca tttgtcattt 2581 gacgaatgaa tctgatgggc acacaacatc gttgtatggt attggatttg gtcccttcat 2641 cattacaaac aagcacttgt ttcgtcgcaa taatggaaca ctgttggtcc aatcactaca 2701 tggtgtattc aaggtcaagg acaccacgac tttgcaacaa cacctcattg atgggaggga 2761 catgatgatt attcgcatgc ctaaggattt cccaccattt cctcaaaagc tgaaatttag 2821 agagccacaa agggaagagc gcatatgtct tgtgacaacc aacttccaag ctcccgagaa 2881 cctgtacttc cagggttcta gagaacagaa gctgatctca gaagaggatc tggcccgggc 2941 catgaagcta ctgtcttcta tcgaacaagc atgcgatatt tgccgactta aaaagctcaa 3001 gtgctccaaa gaaaaaccga agtgcgccaa gtgtctgaag aacaactggg agtgtcgcta 3061 ctctcccaaa accaaaaggt ctccgctgac tagggcacat ctgacagaag tggaatcaag 3121 gctagaaaga ctggaacagc tatttctact gatttttcct cgagaagacc ttgacatgat 3181 tttgaaaatg gattctttac aggatataaa agcattgtta acaggattat ttgtacaaga 3241 taatgtgaat aaagatgccg tcacagatag attggcttca gtggagactg atatgcctct 3301 aacattgaga cagcatagaa taagtgcgac atcatcatcg gaagagagta gtaacaaagg 3361 tcaaagacag ttgactgtat cgccggaatt cccggggatc agcttgataa ttcgattcac 3421 catgcccaag aagaagagga aggtggcgta cagccgcgcg cgtacgaaaa acaattacgg 3481 gtctaccatc gagggcctgc tcgatctccc ggacgacgac gcccccgaag aggcggggct 3541 ggcggctccg cgcctgtcct ttctccccgc gggacacacg cgcagactgt cgacggcccc 3601 cccgaccgat gtcagcctgg gggacgagct ccacttagac ggcgaggacg tcgcgatggc 3661 gcatgccgac gcgctagacg atttcgatct ggacatgttg ggggacgggg attccccggg 3721 tccgggattt accccccacg actccgcccc ctacggcgct ctggatatgg ccgacttcga 3781 gtttgagcag atgtttaccg atgcccttgg aattgacgag tacggtgggg gctacccata 3841 cgatgttcct gactatgcgg gctatcccta tgacgtcccg gactatgcat gagggcccta 3901 ttctatagtg tcacctaaat gctagagctc gctgatcagc ctcgactgtg ccttctagtt 3961 gccagccatc tgttgtttgc ccctcccccg tgccttcctt gaccctggaa ggtgccactc 4021 ccactgtcct ttcctaataa aatgaggaaa ttgcatcgca ttgtctgagt aggtgtcatt 4081 ctattctggg gggtggggtg gggcaggaca gcaaggggga ggattgggaa gacaatagca 4141 ggcatgctgg ggatgcggtg ggctctatgg cttctgaggc ggaaagaacc agctggggct 4201 ctagggggta tccccacgcg ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta 4261 cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc cgctcctttc gctttcttcc 4321 cttcctttct cgccacgttc gccggctttc cccgtcaagc tctaaatcgg ggcatccctt 4381 tagggttccg atttagtgct ttacggcacc tcgaccccaa aaaacttgat tagggtgatg 4441 gttcacgtag tgggccatcg ccctgataga cggtttttcg ccctttgacg ttggagtcca 4501 cgttctttaa tagtggactc ttgttccaaa ctggaacaac actcaaccct atctcggtct 4561 attcttttga tttataaggg attttgggga tttcggccta ttggttaaaa aatgagctga 4621 tttaacaaaa atttaacgcg aattaattct gtggaatgtg tgtcagttag ggtgtggaaa 4681 gtccccaggc tccccaggca ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa 4741 ccaggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcatctca 4801 attagtcagc aaccatagtc ccgcccctaa ctccgcccat cccgccccta actccgccca 4861 gttccgccca ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg 4921 ccgcctctgc ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggct 4981 tttgcaaaaa gctcccggga gcttgtatat ccattttcgg atctgatcaa gagacaggat 5041 gaggatcgtt tcgcatgatt gaacaagatg gattgcacgc aggttctccg gccgcttggg 5101 tggagaggct attcggctat gactgggcac aacagacaat cggctgctct gatgccgccg 5161 tgttccggct gtcagcgcag gggcgcccgg ttctttttgt caagaccgac ctgtccggtg 5221 ccctgaatga actgcaggac gaggcagcgc ggctatcgtg gctggccacg acgggcgttc 5281 cttgcgcagc tgtgctcgac gttgtcactg aagcgggaag ggactggctg ctattgggcg 5341 aagtgccggg gcaggatctc ctgtcatctc accttgctcc tgccgagaaa gtatccatca 5401 tggctgatgc aatgcggcgg ctgcatacgc ttgatccggc tacctgccca ttcgaccacc 5461 aagcgaaaca tcgcatcgag cgagcacgta ctcggatgga agccggtctt gtcgatcagg 5521 atgatctgga cgaagagcat caggggctcg cgccagccga actgttcgcc aggctcaagg 5581 cgcgcatgcc cgacggcgag gatctcgtcg tgacccatgg cgatgcctgc ttgccgaata 5641 tcatggtgga aaatggccgc ttttctggat tcatcgactg tggccggctg ggtgtggcgg 5701 accgctatca ggacatagcg ttggctaccc gtgatattgc tgaagagctt ggcggcgaat 5761 gggctgaccg cttcctcgtg ctttacggta tcgccgctcc cgattcgcag cgcatcgcct 5821 tctatcgcct tcttgacgag ttcttctgag cgggactctg gggttcgaaa tgaccgacca 5881 agcgacgccc aacctgccat cacgagattt cgattccacc gccgccttct atgaaaggtt 5941 gggcttcgga atcgttttcc gggacgccgg ctggatgatc ctccagcgcg gggatctcat 6001 gctggagttc ttcgcccacc ccaacttgtt tattgcagct tataatggtt acaaataaag 6061 caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt 6121 gtccaaactc atcaatgtat cttatcatgt ctgtataccg tcgacctcta gctagagctt 6181 ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt tatccgctca caattccaca 6241 caacatacga gccggaagca taaagtgtaa agcctggggt gcctaatgag tgagctaact 6301 cacattaatt gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt cgtgccagct 6361 gcattaatga atcggccaac gcgcggggag aggcggtttg cgtattgggc gctcttccgc 6421 ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca 6481 ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg 6541 agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca 6601 taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa 6661 cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc 6721 tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc 6781 gctttctcaa tgctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct 6841 gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg 6901 tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag 6961 gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta 7021 cggctacact agaaggacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg 7081 aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt 7141 tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt 7201 ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag 7261 attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat 7321 ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc 7381 tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat 7441 aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc 7501 acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag 7561 aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag 7621 agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt 7681 ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg 7741 agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt 7801 tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc 7861 tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc 7921 attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa 7981 taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg 8041 aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc 8101 caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag 8161 gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt 8221 cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt 8281 tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc 8341 acctgacgtc //