LOCUS       Exported                5676 bp ds-DNA     circular SYN 15-JUN-2021
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5676)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Monday, Nov 14, 2022 from SnapGene 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..5676
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     enhancer        31..133
                     /label=5x GAL4/UAS
     misc_feature    31..47
                     /label=1xUAS segment, CGGRNNRCYNYNCNCCG
                     /note="1xUAS segment, CGGRNNRCYNYNCNCCG"
     misc_feature    50..66
                     /label=1xUAS segment, CGGRNNRCYNYNCNCCG
                     /note="1xUAS segment, CGGRNNRCYNYNCNCCG"
     misc_feature    74..90
                     /label=1xUAS segment, CGGRNNRCYNYNCNCCG
                     /note="1xUAS segment, CGGRNNRCYNYNCNCCG"
     misc_feature    93..109
                     /label=1xUAS segment, CGGRNNRCYNYNCNCCG
                     /note="1xUAS segment, CGGRNNRCYNYNCNCCG"
     misc_feature    117..133
                     /label=1xUAS segment, CGGRNNRCYNYNCNCCG
                     /note="1xUAS segment, CGGRNNRCYNYNCNCCG"
     enhancer        165..267
                     /label=5x GAL4/UAS
     misc_feature    165..181
                     /label=1xUAS segment, CGGRNNRCYNYNCNCCG
                     /note="1xUAS segment, CGGRNNRCYNYNCNCCG"
     misc_feature    184..200
                     /label=1xUAS segment, CGGRNNRCYNYNCNCCG
                     /note="1xUAS segment, CGGRNNRCYNYNCNCCG"
     misc_feature    208..224
                     /label=1xUAS segment, CGGRNNRCYNYNCNCCG
                     /note="1xUAS segment, CGGRNNRCYNYNCNCCG"
     misc_feature    227..243
                     /label=1xUAS segment, CGGRNNRCYNYNCNCCG
                     /note="1xUAS segment, CGGRNNRCYNYNCNCCG"
     misc_feature    251..267
                     /label=1xUAS segment, CGGRNNRCYNYNCNCCG
                     /note="1xUAS segment, CGGRNNRCYNYNCNCCG"
     promoter        279..319
                     /label=Adenovirus major late promoter
                     /note="Adenovirus major late promoter"
     CDS             372..2024
                     /codon_start=1
                     /product="firefly luciferase"
                     /label=luciferase luc2
                     /note="synthetic luc2 version of the luciferase gene"
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
     primer_bind     633..650
                     /label=luc2_pGL4_1 forw
     primer_bind     714..731
                     /label=luc2_pGL4_1 rev
     primer_bind     935..952
                     /label=luc2_pGL4_2 forw
     primer_bind     1233..1250
                     /label=luc2_pGL4_3 forw
     primer_bind     1546..1564
                     /label=luc2_pGL4_4 forw
     primer_bind     1833..1850
                     /label=luc2_pGL4_5 forw
     polyA_signal    2059..2280
                     /label=SV40 late poly(A) signal
                     /note="SV40 late poly(A) signal"
     polyA_signal    2068..2189
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     promoter        2328..2746
                     /label=SV40 early enhancer/promoter
                     /note="SV40 early enhancer/promoter"
     promoter        2383..2740
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      2591..2726
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             2771..3370
                     /codon_start=1
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     polyA_signal    3395..3443
                     /label=synthetic poly(A) signal
                     /note="synthetic poly(A) signal"
     primer_bind     3510..3529
                     /label=Reporter Vector primer 4
                     /note="Reporter Vector primer 4 (RVprimer4) binding region"
     rep_origin      3767
                     /direction=RIGHT
                     /label=ColEI-derived plasmid replication origin
                     /note="ColEI-derived plasmid replication origin"
     rep_origin      complement(3770..4358)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(4558..5418)
                     /codon_start=1
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     polyA_signal    5523..5676
                     /label=synthetic poly
                     /note="synthetic poly(A) signal/transcriptional pause site"
     polyA_signal    5523..5571
                     /note="synthetic polyadenylation signal"
     misc_feature    5585..5676
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from 
                     the human alpha-2 globin gene"
     primer_bind     5625..5644
                     /label=Reporter Vector primer 3
                     /note="Reporter Vector primer 3 (RVprimer3) binding region"
ORIGIN
        1 ggcctaactg gccggtaccg agtttctaga cggagtactg tcctccgagc ggagtactgt
       61 cctccgactc gagcggagta ctgtcctccg atcggagtac tgtcctccgc gaattccgga
      121 gtactgtcct ccgaagacgc tagtgccggt accgagtttc tagacggagt actgtcctcc
      181 gagcggagta ctgtcctccg actcgagcgg agtactgtcc tccgatcgga gtactgtcct
      241 ccgcgaattc cggagtactg tcctccgaag acgctagcgg ggggctataa aagggggtgg
      301 gggcgttcgt cctcactcta gatctgcgat ctaagtaagc ttggcaatcc ggtactgttg
      361 gtaaagccac catggaagat gccaaaaaca ttaagaaggg cccagcgcca ttctacccac
      421 tcgaagacgg gaccgccggc gagcagctgc acaaagccat gaagcgctac gccctggtgc
      481 ccggcaccat cgcctttacc gacgcacata tcgaggtgga cattacctac gccgagtact
      541 tcgagatgag cgttcggctg gcagaagcta tgaagcgcta tgggctgaat acaaaccatc
      601 ggatcgtggt gtgcagcgag aatagcttgc agttcttcat gcccgtgttg ggtgccctgt
      661 tcatcggtgt ggctgtggcc ccagctaacg acatctacaa cgagcgcgag ctgctgaaca
      721 gcatgggcat cagccagccc accgtcgtat tcgtgagcaa gaaagggctg caaaagatcc
      781 tcaacgtgca aaagaagcta ccgatcatac aaaagatcat catcatggat agcaagaccg
      841 actaccaggg cttccaaagc atgtacacct tcgtgacttc ccatttgcca cccggcttca
      901 acgagtacga cttcgtgccc gagagcttcg accgggacaa aaccatcgcc ctgatcatga
      961 acagtagtgg cagtaccgga ttgcccaagg gcgtagccct accgcaccgc accgcttgtg
     1021 tccgattcag tcatgcccgc gaccccatct tcggcaacca gatcatcccc gacaccgcta
     1081 tcctcagcgt ggtgccattt caccacggct tcggcatgtt caccacgctg ggctacttga
     1141 tctgcggctt tcgggtcgtg ctcatgtacc gcttcgagga ggagctattc ttgcgcagct
     1201 tgcaagacta taagattcaa tctgccctgc tggtgcccac actatttagc ttcttcgcta
     1261 agagcactct catcgacaag tacgacctaa gcaacttgca cgagatcgcc agcggcgggg
     1321 cgccgctcag caaggaggta ggtgaggccg tggccaaacg cttccaccta ccaggcatcc
     1381 gccagggcta cggcctgaca gaaacaacca gcgccattct gatcaccccc gaaggggacg
     1441 acaagcctgg cgcagtaggc aaggtggtgc ccttcttcga ggctaaggtg gtggacttgg
     1501 acaccggtaa gacactgggt gtgaaccagc gcggcgagct gtgcgtccgt ggccccatga
     1561 tcatgagcgg ctacgttaac aaccccgagg ctacaaacgc tctcatcgac aaggacggct
     1621 ggctgcacag cggcgacatc gcctactggg acgaggacga gcacttcttc atcgtggacc
     1681 ggctgaagag cctgatcaaa tacaagggct accaggtagc cccagccgaa ctggagagca
     1741 tcctgctgca acaccccaac atcttcgacg ccggggtcgc cggcctgccc gacgacgatg
     1801 ccggcgagct gcccgccgca gtcgtcgtgc tggaacacgg taaaaccatg accgagaagg
     1861 agatcgtgga ctatgtggcc agccaggtta caaccgccaa gaagctgcgc ggtggtgttg
     1921 tgttcgtgga cgaggtgcct aaaggactga ccggcaagtt ggacgcccgc aagatccgcg
     1981 agattctcat taaggccaag aagggcggca agatcgccgt gtaataattc tagagtcggg
     2041 gcggccggcc gcttcgagca gacatgataa gatacattga tgagtttgga caaaccacaa
     2101 ctagaatgca gtgaaaaaaa tgctttattt gtgaaatttg tgatgctatt gctttatttg
     2161 taaccattat aagctgcaat aaacaagtta acaacaacaa ttgcattcat tttatgtttc
     2221 aggttcaggg ggaggtgtgg gaggtttttt aaagcaagta aaacctctac aaatgtggta
     2281 aaatcgataa ggatccgttt gcgtattggg cgctcttccg ctgatctgcg cagcaccatg
     2341 gcctgaaata acctctgaaa gaggaacttg gttagctacc ttctgaggcg gaaagaacca
     2401 gctgtggaat gtgtgtcagt tagggtgtgg aaagtcccca ggctccccag caggcagaag
     2461 tatgcaaagc atgcatctca attagtcagc aaccaggtgt ggaaagtccc caggctcccc
     2521 agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccatag tcccgcccct
     2581 aactccgccc atcccgcccc taactccgcc cagttccgcc cattctccgc cccatggctg
     2641 actaattttt tttatttatg cagaggccga ggccgcctct gcctctgagc tattccagaa
     2701 gtagtgagga ggcttttttg gaggcctagg cttttgcaaa aagctcgatt cttctgacac
     2761 tagcgccacc atgaccgagt acaagcctac cgtgcgcctg gccactcgcg atgatgtgcc
     2821 ccgcgccgtc cgcactctgg ccgccgcttt cgccgactac cccgctaccc ggcacaccgt
     2881 ggaccccgac cggcacatcg agcgtgtgac agagttgcag gagctgttcc tgacccgcgt
     2941 cgggctggac atcggcaagg tgtgggtagc cgacgacggc gcggccgtgg ccgtgtggac
     3001 tacccccgag agcgttgagg ccggcgccgt gttcgccgag atcggccccc gaatggccga
     3061 gctgagcggc agccgcctgg ccgcccagca gcaaatggag ggcctgcttg ccccccatcg
     3121 tcccaaggag cctgcctggt ttctggccac tgtaggagtg agccccgacc accagggcaa
     3181 gggcttgggc agcgccgtcg tgttgcccgg cgtagaggcc gccgaacgcg ccggtgtgcc
     3241 cgcctttctc gaaacaagcg caccaagaaa ccttccattc tacgagcgcc tgggcttcac
     3301 cgtgaccgcc gatgtcgagg tgcccgaggg acctaggacc tggtgtatga cacgaaaacc
     3361 tggcgcctaa tgatctagaa ccggtcatgg ccgcaataaa atatctttat tttcattaca
     3421 tctgtgtgtt ggttttttgt gtgttcgaac tagatgctgt cgaccgatgc ccttgagagc
     3481 cttcaaccca gtcagctcct tccggtgggc gcggggcatg actatcgtcg ccgcacttat
     3541 gactgtcttc tttatcatgc aactcgtagg acaggtgccg gcagcgctct tccgcttcct
     3601 cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa
     3661 aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa
     3721 aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc
     3781 tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga
     3841 caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc
     3901 cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt
     3961 ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct
     4021 gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg
     4081 agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta
     4141 gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct
     4201 acactagaag aacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa
     4261 gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt
     4321 gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta
     4381 cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat
     4441 caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa
     4501 gtatatatga gtaaacttgg tctgacagcg gccgcaaatg ctaaaccact gcagtggtta
     4561 ccagtgcttg atcagtgagg caccgatctc agcgatctgc ctatttcgtt cgtccatagt
     4621 ggcctgactc cccgtcgtgt agatcactac gattcgtgag ggcttaccat caggccccag
     4681 cgcagcaatg atgccgcgag agccgcgttc accggccccc gatttgtcag caatgaacca
     4741 gccagcaggg agggccgagc gaagaagtgg tcctgctact ttgtccgcct ccatccagtc
     4801 tatgagctgc tgtcgtgatg ctagagtaag aagttcgcca gtgagtagtt tccgaagagt
     4861 tgtggccatt gctactggca tcgtggtatc acgctcgtcg ttcggtatgg cttcgttcaa
     4921 ctctggttcc cagcggtcaa gccgggtcac atgatcaccc atattatgaa gaaatgcagt
     4981 cagctcctta gggcctccga tcgttgtcag aagtaagttg gccgcggtgt tgtcgctcat
     5041 ggtaatggca gcactacaca attctcttac cgtcatgcca tccgtaagat gcttttccgt
     5101 gaccggcgag tactcaacca agtcgttttg tgagtagtgt atacggcgac caagctgctc
     5161 ttgcccggcg tctatacggg acaacaccgc gccacatagc agtactttga aagtgctcat
     5221 catcgggaat cgttcttcgg ggcggaaaga ctcaaggatc ttgccgctat tgagatccag
     5281 ttcgatatag cccactcttg cacccagttg atcttcagca tcttttactt tcaccagcgt
     5341 ttcggggtgt gcaaaaacag gcaagcaaaa tgccgcaaag aagggaatga gtgcgacacg
     5401 aaaatgttgg atgctcatac tcgtcctttt tcaatattat tgaagcattt atcagggtta
     5461 ctagtacgtc tctcaaggat aagtaagtaa tattaaggta cgggaggtat tggacaggcc
     5521 gcaataaaat atctttattt tcattacatc tgtgtgttgg ttttttgtgt gaatcgatag
     5581 tactaacata cgctctccat caaaacaaaa cgaaacaaaa caaactagca aaataggctg
     5641 tccccagtgc aagtgcaggt gccagaacat ttctct
//
