LOCUS       Exported                4244 bp ds-DNA     circular SYN 01-NOV-2022
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4244)
  AUTHORS   Luca Torello Pianale
  TITLE     Direct Submission
  JOURNAL   Exported Nov 1, 2022 from SnapGene 4.1.9
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..4244
                     /organism="recombinant plasmid"
                     /mol_type="other DNA"
     protein_bind    complement(join(20..23,25..30))
                     /label=BsmBI
     misc_feature    31..173
                     /label=ConS
     promoter        185..314
                     /label=BBa_J72163 GlpT Promoter
     RBS             315..350
                     /label=sfGFP Ribosome Binding Site
     CDS             351..1067
                     /label=sfGFP
     terminator      1068..1196
                     /label=BBa_B0015 Terminator
     misc_feature    1208..1350
                     /label=ConE
     protein_bind    join(1351..1356,1358..1361)
                     /label=BsmBI
     misc_feature    1358..1382
                     /label=ConE scar
     misc_feature    1402..1421
                     /label=Dummy
     misc_feature    1426..1445
                     /label=HO 3' barcode
     misc_feature    1446..1945
                     /label=HO 3' Homology
     terminator      complement(1958..1984)
                     /label=KanR Terminator
     CDS             complement(1985..2800)
                     /label=KanR
     promoter        complement(2801..2948)
                     /label=KanR Promoter
     rep_origin      complement(2949..3712)
                     /direction=LEFT
                     /label=ColE1
     misc_feature    3725..4224
                     /label=HO 5' Homology
     misc_feature    complement(4225..4244)
                     /label=HO 5' barcode
ORIGIN
        1 ccctgaattc gcatctagac tgatgagacg tggtagagcc acaaacagcc ggtacaagca
       61 acgatctcca ggaccatctg aatcatgcgc ggatgacacg aactcacgac ggcgatcaca
      121 gacattaacc cacagtacag acactgcgac aacgtggcaa ttcgtcgcaa tacaacgtga
      181 gaccgaaagt gaaacgtgat ttcatgcgtc attttgaaca ttttgtaaat cttatttaat
      241 aatgtgtgcg gcaattcaca tttaatttat gaatgttttc ttaacatcgc ggcaactcaa
      301 gaaacggcag gttcggatct tagctactag agaaagagga gaaatactag atgcgtaaag
      361 gcgaagagct gttcactggt gtcgtcccta ttctggtgga actggatggt gatgtcaacg
      421 gtcataagtt ttccgtgcgt ggcgagggtg aaggtgacgc aactaatggt aaactgacgc
      481 tgaagttcat ctgtactact ggtaaactgc cggttccttg gccgactctg gtaacgacgc
      541 tgacttatgg tgttcagtgc tttgctcgtt atccggacca tatgaagcag catgacttct
      601 tcaagtccgc catgccggaa ggctatgtgc aggaacgcac gatttccttt aaggatgacg
      661 gcacgtacaa aacgcgtgcg gaagtgaaat ttgaaggcga taccctggta aaccgcattg
      721 agctgaaagg cattgacttt aaagaggacg gcaatatcct gggccataag ctggaataca
      781 attttaacag ccacaatgtt tacatcaccg ccgataaaca aaaaaatggc attaaagcga
      841 attttaaaat tcgccacaac gtggaggatg gcagcgtgca gctggctgat cactaccagc
      901 aaaacactcc aatcggtgat ggtcctgttc tgctgccaga caatcactat ctgagcacgc
      961 aaagcgttct gtctaaagat ccgaacgaga aacgcgatca tatggttctg ctggagttcg
     1021 taaccgcagc gggcatcacg catggtatgg atgaactgta caaatgacca ggcatcaaat
     1081 aaaacgaaag gctcagtcga aagactgggc ctttcgtttt atctgttgtt tgtcggtgaa
     1141 cgctctctac tagagtcaca ctggctcacc ttcgggtggg cctttctgcg tttataggtc
     1201 tcagctggaa atctgctcgt cagtggtgct cacactgacg aatcatgtac agatcatacc
     1261 gatgactgcc tggcgactca caactaagca agacagccgg aaccagcgcc ggcgaacacc
     1321 actgcatata tggcatatca caacagtcca cgtctcaagc agttacagag atgttacgaa
     1381 ccactagtgc actgcagtac ataaccccta ggtcttcctg agagttatcg tgttgcatct
     1441 gcggctttaa attgatgtat ctcatcgcag gcacgggcag tacagtgccc tgagcgtagg
     1501 gaaaaatgaa aaaaaggatg taacttttaa cataattcca gcacgcagcg attgggtata
     1561 atgaagattg ttaagttcaa caacatgtga aattcttagc tccgcaactt gcttgaatta
     1621 tgagctctaa gattcaagaa gtaaataggt catataatgg aatgatagta gtacttgata
     1681 aaggaaccat gtgatcttac gttgatatga aaataattct ctcacagaag tcagtttgta
     1741 tgactaacat agagagtaat tgcttttcgg ataaaatcgc acctaacaaa gattaccttt
     1801 tgtaaagcct ccagaacagc tatgaatgtt cgttattcta cgcactttct ttcatacatc
     1861 ttgatccgcc ttttttgatg taggttttaa cgcggcaaaa catattaatt tttctgattt
     1921 ggaaaaataa agagttagtt ctgccccgag cggccgcgtg ttacaaccaa ttaaccaatt
     1981 ctgattagaa aaactcatcg agcatcaaat gaaactgcaa tttattcata tcaggattat
     2041 caataccata tttttgaaaa agccgtttct gtaatgaagg agaaaactca ccgaggcagt
     2101 tccataggat ggcaagatcc tggtatcggt ctgcgattcc gactcgtcca acatcaatac
     2161 aacctattaa tttcccctcg tcaaaaataa ggttatcaag tgagaaatca ccatgagtga
     2221 cgactgaatc cggtgagaat ggcaaaagct tatgcatttc tttccagact tgttcaacag
     2281 gccagccatt acgctcgtca tcaaaatcac tcgcatcaac caaaccgtta ttcattcgtg
     2341 attgcgcctg agcgaggcga aatacgcgat cgctgttaaa aggacaatta caaacaggaa
     2401 tcgaatgcaa ccggcgcagg aacactgcca gcgcatcaac aatattttca cctgaatcag
     2461 gatattcttc taatacctgg aatgctgttt tcccggggat cgcagtggtg agtaaccatg
     2521 catcatcagg agtacggata aaatgcttga tggtcggaag aggcataaat tccgtcagcc
     2581 agtttagtct gaccatctca tctgtaacat cattggcaac gctacctttg ccatgtttca
     2641 gaaacaactc tggcgcatcg ggcttcccat acaatcgata gattgtcgca cctgattgcc
     2701 cgacattatc gcgagcccat ttatacccat ataaatcagc atccatgttg gaatttaatc
     2761 gcggcctgga gcaagacgtt tcccgttgaa tatggctcat aacacccctt gtattactgt
     2821 ttatgtaagc agacagtttt attgttcatg atgatatatt tttatcttgt gcaatgtaac
     2881 atcagagatt ttgagacaca acgtggcttt gttgaataaa tcgaactttt gctgagttga
     2941 aggatcagtc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc
     3001 cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt
     3061 gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac
     3121 tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg ttcttctagt
     3181 gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct
     3241 gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga
     3301 ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac
     3361 acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg
     3421 agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt
     3481 cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc
     3541 tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg
     3601 gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc
     3661 ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc gtgcggccgc
     3721 caattctaat tgtatcgaga tcacttttcg tgatccgcta atcagcgacg gtcacattag
     3781 gtttgccaag tcagggtatg aaccatacga tcagttttcg tgaacctggt acgtatattg
     3841 tggcgtttgt gtatattttc attctttgac aacaatcaat accaacctca aataggaaaa
     3901 gtaataagtt tggcgttaca ccccaaaaga cgccaaacgg atcgaactta ctcaatagca
     3961 attagcgaga caaaacctac gttaagacct gtaaccgatt tatcaaagca ctctgcggtt
     4021 ctttcttggg aatattacct ggacattttg tgccctcaag aaacgaggct ctacgagcct
     4081 gttggagccc ctcagacatt agccgccacg aatcaaactt tttacgcgat tcggcccaaa
     4141 tcagtttctc acagatcatt cgtagagtga aaaagcacat cgattatttg ataccccttt
     4201 gggttaatta ctgttgaggt ctttgccgta agttccatcg ctgt
//
