LOCUS       Exported                3499 bp ds-DNA     circular SYN 01-NOV-2022
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3499)
  AUTHORS   Yvonne Nygard
  TITLE     Direct Submission
  JOURNAL   Exported Nov 1, 2022 from SnapGene 4.1.9
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..3499
                     /organism="recombinant plasmid"
                     /mol_type="other DNA"
     misc_feature    20..162
                     /label=Con3
     protein_bind    join(163..168,170..173)
                     /label=BsmBI
     misc_feature    170..194
                     /label=Con3 scar
     promoter        206..335
                     /label=BBa_J72163 GlpT Promoter
     RBS             336..371
                     /label=sfGFP Ribosome Binding Site
     CDS             372..1088
                     /label=sfGFP
     terminator      1089..1217
                     /label=BBa_B0015 Terminator
     protein_bind    complement(join(1229..1232,1234..1239))
                     /label=BsmBI
     misc_feature    1240..1382
                     /label=Con4
     misc_feature    1402..1421
                     /label=Dummy
     misc_feature    1426..1525
                     /label=Homology Arm 3' X2
     terminator      complement(1538..1634)
                     /label=AmpR Terminator
     CDS             complement(1635..2492)
                     /label=AmpR
     promoter        complement(2493..2623)
                     /label=AmpR Promoter
     rep_origin      complement(2624..3387)
                     /direction=LEFT
                     /label=ColE1
     misc_feature    3400..3499
                     /label=Homology Arm 5' X2
ORIGIN
        1 ccctgaattc gcatctagag cagacctaga ccacatgagg ctgatgtagg acagccacca
       61 gtggcagcta atcaacaccg caagatgccg atgcacgctc atatcatcgt cgtcagcctg
      121 gtagccattc gacatacgga tcagggaact cgaacccagt accgtctcag ttctgtatgg
      181 gcacagacaa cctaaacgtg agaccgaaag tgaaacgtga tttcatgcgt cattttgaac
      241 attttgtaaa tcttatttaa taatgtgtgc ggcaattcac atttaattta tgaatgtttt
      301 cttaacatcg cggcaactca agaaacggca ggttcggatc ttagctacta gagaaagagg
      361 agaaatacta gatgcgtaaa ggcgaagagc tgttcactgg tgtcgtccct attctggtgg
      421 aactggatgg tgatgtcaac ggtcataagt tttccgtgcg tggcgagggt gaaggtgacg
      481 caactaatgg taaactgacg ctgaagttca tctgtactac tggtaaactg ccggttcctt
      541 ggccgactct ggtaacgacg ctgacttatg gtgttcagtg ctttgctcgt tatccggacc
      601 atatgaagca gcatgacttc ttcaagtccg ccatgccgga aggctatgtg caggaacgca
      661 cgatttcctt taaggatgac ggcacgtaca aaacgcgtgc ggaagtgaaa tttgaaggcg
      721 ataccctggt aaaccgcatt gagctgaaag gcattgactt taaagaggac ggcaatatcc
      781 tgggccataa gctggaatac aattttaaca gccacaatgt ttacatcacc gccgataaac
      841 aaaaaaatgg cattaaagcg aattttaaaa ttcgccacaa cgtggaggat ggcagcgtgc
      901 agctggctga tcactaccag caaaacactc caatcggtga tggtcctgtt ctgctgccag
      961 acaatcacta tctgagcacg caaagcgttc tgtctaaaga tccgaacgag aaacgcgatc
     1021 atatggttct gctggagttc gtaaccgcag cgggcatcac gcatggtatg gatgaactgt
     1081 acaaatgacc aggcatcaaa taaaacgaaa ggctcagtcg aaagactggg cctttcgttt
     1141 tatctgttgt ttgtcggtga acgctctcta ctagagtcac actggctcac cttcgggtgg
     1201 gcctttctgc gtttataggt ctcagctggg tatgagacgg cctgcactag acgaactagg
     1261 caagatgcgt ccaatccgtc taaacatggt gacaacgctg gacagatgac gtaacaccga
     1321 gccacatcct gaaatcgagg caggctaacc gaaaccgtga caatgcaaag agacagcctg
     1381 acactagtgc actgcagtac ataaccccta ggtcttcctg agagtgacga aggctaaggt
     1441 cacttcttcg tttcctttat tggggtttcc gtgtagcctt cccctgaata gtgtgggacg
     1501 ttttatgaga agccgtaaga aatagccgag cggccgcgat tatcaaaaag gatcttcacc
     1561 tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact
     1621 tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt
     1681 cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta
     1741 ccatctggcc ccagtgctgc aatgataccg cgggacccac gctcaccggc tccagattta
     1801 tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc
     1861 gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat
     1921 agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt
     1981 atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg
     2041 tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca
     2101 gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta
     2161 agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg
     2221 cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca tagcagaact
     2281 ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg
     2341 ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt
     2401 actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga
     2461 ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc
     2521 atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa
     2581 caaatagggg ttccgcgcac atttccccga aaagtgccac ctgtcatgac caaaatccct
     2641 taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct
     2701 tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca
     2761 gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc
     2821 agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc
     2881 aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct
     2941 gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag
     3001 gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc
     3061 tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg
     3121 agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag
     3181 cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt
     3241 gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac
     3301 gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg
     3361 ttatcccctg attctgtgga taaccgtgcg gccgccaatg tcaaaagatc ctctcatacc
     3421 atattaagta aattgcctcc atttcttttt cctcgggcag agaaactcgc aggcaacttg
     3481 ctctcgaagt ggtcacgtg
//
