LOCUS       Exported                2797 bp ds-DNA     circular SYN 01-NOV-2022
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2797)
  AUTHORS   Yvonne Nygard
  TITLE     Direct Submission
  JOURNAL   Exported Nov 1, 2022 from SnapGene 4.1.9
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..2797
                     /organism="recombinant plasmid"
                     /mol_type="other DNA"
     terminator      complement(13..109)
                     /label=AmpR Terminator
     CDS             complement(110..967)
                     /label=AmpR
     promoter        complement(968..1098)
                     /label=AmpR Promoter
     rep_origin      complement(1099..1862)
                     /direction=LEFT
                     /label=ColE1
     misc_feature    1875..1974
                     /label=RPL13A 5' Homology Arm
     misc_feature    1975..1980
                     /label=Ser-Gly Linker
     CDS             1981..2694
                     /label=mTurquoise2-Tag
                     /label=mTurquoise2
     misc_feature    2698..2797
                     /label=RPL13A 3' Homology Arm
ORIGIN
        1 ccgagcggcc gcgattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa
       61 gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa
      121 tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc
      181 ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga
      241 taccgcggga cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa
      301 gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt
      361 gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg
      421 ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc
      481 aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg
      541 gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag
      601 cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt
      661 actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt
      721 caatacggga taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac
      781 gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac
      841 ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag
      901 caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa
      961 tactcatact cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga
     1021 gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc
     1081 cccgaaaagt gccacctgtc atgaccaaaa tcccttaacg tgagttttcg ttccactgag
     1141 cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa
     1201 tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag
     1261 agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg
     1321 ttcttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat
     1381 acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta
     1441 ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg
     1501 gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc
     1561 gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa
     1621 gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc
     1681 tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt
     1741 caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct
     1801 tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc
     1861 gtgcggccgc caattttcag aaccttgaga ttagccagat ctgagaagaa attcagaggt
     1921 atcagagaaa agagagctag agaaaaggct gaagctgaag ctgaaaagaa gaaaggatcc
     1981 gtttctaaag gtgaagaatt attcactggt gttgtcccaa ttttggttga attagatggt
     2041 gatgttaatg gtcacaaatt ttctgtctcc ggtgaaggtg aaggtgatgc tacttacggt
     2101 aaattgacct taaaatttat ttgtactact ggtaaattgc cagttccatg gccaacctta
     2161 gtcactactt tatcttgggg tgttcaatgt tttgcaagat acccagatca tatgaaacaa
     2221 catgactttt tcaagtctgc catgccagaa ggttatgttc aagaaagaac tatttttttc
     2281 aaagatgacg gtaactacaa gaccagagct gaagtcaagt ttgaaggtga taccttagtt
     2341 aatagaatcg aattaaaagg tattgatttt aaagaagatg gtaacatttt aggtcacaaa
     2401 ttggaataca attatttctc tgacaatgtt tacatcactg ctgacaaaca aaagaatggt
     2461 atcaaagcta acttcaaaat tagacacaac attgaagatg gtggtgttca attagctgac
     2521 cattatcaac aaaatactcc aattggtgat ggtccagtct tgttaccaga caaccattac
     2581 ttatccactc aatctaagtt atccaaagat ccaaacgaaa agagggacca catggtcttg
     2641 ttagaatttg ttactgctgc tggtattacc ttgggtatgg atgaattgta caaataaatc
     2701 tgttcaattt cataaataat atatatgtac aatgtataaa cgggaagcca tcacttataa
     2761 tttatcattt ccttaacttt atagtctggg tgagaat
//
