LOCUS       Exported                4339 bp ds-DNA     circular SYN 31-OCT-2022
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4339)
  AUTHORS   Yvonne Nygard
  TITLE     Direct Submission
  JOURNAL   Exported Oct 31, 2022 from SnapGene 4.1.9
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..4339
                     /organism="recombinant plasmid"
                     /mol_type="other DNA"
     misc_feature    20..162
                     /label=Con2
     protein_bind    join(163..168,170..173)
                     /label=BsmBI
     misc_feature    174..194
                     /label=Con2 scar
     promoter        206..335
                     /label=BBa_J72163 GlpT Promoter
     RBS             336..371
                     /label=sfGFP Ribosome Binding Site
     CDS             372..1088
                     /label=sfGFP
     terminator      1089..1217
                     /label=BBa_B0015 Terminator
     protein_bind    complement(join(1229..1232,1234..1239))
                     /label=BsmBI
     misc_feature    1240..1382
                     /label=ConE
     misc_feature    1402..1421
                     /label=Dummy
     misc_feature    1426..1445
                     /label=HO 3' barcode
     misc_feature    1446..1945
                     /label=HO 3' Homology
     terminator      complement(1958..2054)
                     /label=AmpR Terminator
     CDS             complement(2055..2912)
                     /label=AmpR
     promoter        complement(2913..3043)
                     /label=AmpR Promoter
     rep_origin      complement(3044..3807)
                     /direction=LEFT
                     /label=ColE1
     misc_feature    3820..4319
                     /label=HO 5' Homology
     misc_feature    complement(4320..4339)
                     /label=HO 5' barcode
ORIGIN
        1 ccctgaattc gcatctagaa taaatcgcag ccaagtgagt gaatagatga cgcaccacgg
       61 tcagacacgg ccacatcgta tctcacagga gcaagcgcga taggagcact cacacatagt
      121 acggtgatcc gctgactcct ttgcccaaat aagacgtgag cccgtctcag atgcacacac
      181 tggcttaaga tgacaacgtg agaccgaaag tgaaacgtga tttcatgcgt cattttgaac
      241 attttgtaaa tcttatttaa taatgtgtgc ggcaattcac atttaattta tgaatgtttt
      301 cttaacatcg cggcaactca agaaacggca ggttcggatc ttagctacta gagaaagagg
      361 agaaatacta gatgcgtaaa ggcgaagagc tgttcactgg tgtcgtccct attctggtgg
      421 aactggatgg tgatgtcaac ggtcataagt tttccgtgcg tggcgagggt gaaggtgacg
      481 caactaatgg taaactgacg ctgaagttca tctgtactac tggtaaactg ccggttcctt
      541 ggccgactct ggtaacgacg ctgacttatg gtgttcagtg ctttgctcgt tatccggacc
      601 atatgaagca gcatgacttc ttcaagtccg ccatgccgga aggctatgtg caggaacgca
      661 cgatttcctt taaggatgac ggcacgtaca aaacgcgtgc ggaagtgaaa tttgaaggcg
      721 ataccctggt aaaccgcatt gagctgaaag gcattgactt taaagaggac ggcaatatcc
      781 tgggccataa gctggaatac aattttaaca gccacaatgt ttacatcacc gccgataaac
      841 aaaaaaatgg cattaaagcg aattttaaaa ttcgccacaa cgtggaggat ggcagcgtgc
      901 agctggctga tcactaccag caaaacactc caatcggtga tggtcctgtt ctgctgccag
      961 acaatcacta tctgagcacg caaagcgttc tgtctaaaga tccgaacgag aaacgcgatc
     1021 atatggttct gctggagttc gtaaccgcag cgggcatcac gcatggtatg gatgaactgt
     1081 acaaatgacc aggcatcaaa taaaacgaaa ggctcagtcg aaagactggg cctttcgttt
     1141 tatctgttgt ttgtcggtga acgctctcta ctagagtcac actggctcac cttcgggtgg
     1201 gcctttctgc gtttataggt ctcagctgag catgagacgg aaatctgctc gtcagtggtg
     1261 ctcacactga cgaatcatgt acagatcata ccgatgactg cctggcgact cacaactaag
     1321 caagacagcc ggaaccagcg ccggcgaaca ccactgcata tatggcatat cacaacagtc
     1381 caactagtgc actgcagtac ataaccccta ggtcttcctg agagttatcg tgttgcatct
     1441 gcggctttaa attgatgtat ctcatcgcag gcacgggcag tacagtgccc tgagcgtagg
     1501 gaaaaatgaa aaaaaggatg taacttttaa cataattcca gcacgcagcg attgggtata
     1561 atgaagattg ttaagttcaa caacatgtga aattcttagc tccgcaactt gcttgaatta
     1621 tgagctctaa gattcaagaa gtaaataggt catataatgg aatgatagta gtacttgata
     1681 aaggaaccat gtgatcttac gttgatatga aaataattct ctcacagaag tcagtttgta
     1741 tgactaacat agagagtaat tgcttttcgg ataaaatcgc acctaacaaa gattaccttt
     1801 tgtaaagcct ccagaacagc tatgaatgtt cgttattcta cgcactttct ttcatacatc
     1861 ttgatccgcc ttttttgatg taggttttaa cgcggcaaaa catattaatt tttctgattt
     1921 ggaaaaataa agagttagtt ctgccccgag cggccgcgat tatcaaaaag gatcttcacc
     1981 tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact
     2041 tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt
     2101 cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta
     2161 ccatctggcc ccagtgctgc aatgataccg cgggacccac gctcaccggc tccagattta
     2221 tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc
     2281 gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat
     2341 agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt
     2401 atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg
     2461 tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca
     2521 gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta
     2581 agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg
     2641 cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca tagcagaact
     2701 ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg
     2761 ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt
     2821 actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga
     2881 ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc
     2941 atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa
     3001 caaatagggg ttccgcgcac atttccccga aaagtgccac ctgtcatgac caaaatccct
     3061 taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct
     3121 tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca
     3181 gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc
     3241 agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc
     3301 aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct
     3361 gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag
     3421 gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc
     3481 tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg
     3541 agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag
     3601 cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt
     3661 gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac
     3721 gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg
     3781 ttatcccctg attctgtgga taaccgtgcg gccgccaatt ctaattgtat cgagatcact
     3841 tttcgtgatc cgctaatcag cgacggtcac attaggtttg ccaagtcagg gtatgaacca
     3901 tacgatcagt tttcgtgaac ctggtacgta tattgtggcg tttgtgtata ttttcattct
     3961 ttgacaacaa tcaataccaa cctcaaatag gaaaagtaat aagtttggcg ttacacccca
     4021 aaagacgcca aacggatcga acttactcaa tagcaattag cgagacaaaa cctacgttaa
     4081 gacctgtaac cgatttatca aagcactctg cggttctttc ttgggaatat tacctggaca
     4141 ttttgtgccc tcaagaaacg aggctctacg agcctgttgg agcccctcag acattagccg
     4201 ccacgaatca aactttttac gcgattcggc ccaaatcagt ttctcacaga tcattcgtag
     4261 agtgaaaaag cacatcgatt atttgatacc cctttgggtt aattactgtt gaggtctttg
     4321 ccgtaagttc catcgctgt
//
