LOCUS       Exported                4579 bp ds-DNA     circular SYN 28-OCT-2022
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4579)
  AUTHORS   Luca Torello Pianale
  TITLE     Direct Submission
  JOURNAL   Exported Oct 28, 2022 from SnapGene 4.1.9
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..4579
                     /organism="recombinant plasmid"
                     /mol_type="other DNA"
     misc_feature    20..44
                     /label=ConS scar
     misc_feature    49..146
                     /label=p1xUPRE1-SM
     misc_feature    49..60
                     /label=UPRE1
     misc_feature    61..146
                     /label=Synthetic Minimal Promoter
     CDS             148..864
                     /codon_start=1
                     /label=ymYPET
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KLLCTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIK
                     ANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSYQSKLSKDPNEKRDHMVLL
                     EFLTAAGITLGMDELYK"
     variation       151..153
                     /label=Nucleotide Change
                     /note="Codon 2: from GTC to GTA (both coding for Valine)"
     unsure          871..873
                     /label=STOP CODON
     terminator      880..1135
                     /label=tCYC1
     misc_feature    1140..1164
                     /label=Con1 scar
     promoter        1169..1578
                     /label=pTEFmut8
                     /label=TEF1 promoter mutant 8
     CDS             1580..2290
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVDGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     terminator      2304..2528
                     /label=ScADH1 Terminator
     misc_feature    2533..2557
                     /label=ConE scar
     misc_feature    2577..2596
                     /label=Dummy
     misc_feature    complement(2601..2700)
                     /label=Homology Arm 3' X2
     terminator      complement(2713..2739)
                     /label=KanR Terminator
     CDS             complement(2740..3555)
                     /label=KanR
     promoter        complement(3556..3703)
                     /label=KanR Promoter
     rep_origin      complement(3704..4467)
                     /direction=LEFT
                     /label=ColE1
     misc_feature    4480..4579
                     /label=Homology Arm 5' X2
ORIGIN
        1 ccctgaattc gcatctagac tgaactggcc gataattgca gacgaacggg acagcgtgtc
       61 cttaagatct tgtaatattc taatcaagct tataaaagag cactgttggg cgtgagtgga
      121 ggcgccggaa taaaccatgc aatgaatatg gtatccaagg gcgaagaatt gttcaccggt
      181 gtggtcccaa tcttggtcga gttggacggt gacgtcaacg gtcacaagtt ctctgtttcc
      241 ggtgaaggtg aaggagatgc cacctacggt aaattgacct tgaagttgtt gtgcaccacc
      301 ggcaagctgc cagttccatg gccaacattg gtcaccactt taggttatgg tttacaatgt
      361 tttgctagat atccagatca tatgaaacaa catgatttct tcaaatctgc tatgccagaa
      421 ggttatgttc aagaaagaac tatatttttc aaagatgatg gtaattataa aactagagct
      481 gaagttaaat ttgaaggaga tactttagtt aatagaattg aattgaaagg tattgatttt
      541 aaagaagatg gtaatatttt aggtcataaa ttagaatata attataattc tcataatgtt
      601 tatattacag ctgataaaca aaagaatggt attaaggcta attttaaaat tagacataat
      661 attgaagatg gtggtgttca attagctgat cattatcaac aaaatactcc tattggtgac
      721 ggtccagttt tattacctga taatcattat ttgtcatatc aatctaaatt gtcaaaagat
      781 ccaaatgaaa aaagagatca tatggttttg ttagaatttt tgactgcagc tggtattact
      841 ttaggtatgg atgaattgta taaaggatcc taactcgaga tcgataccgt cgacctcgag
      901 tcatgtaatt agttatgtca cgcttacatt cacgccctcc tcccacatcc gctctaaccg
      961 aaaaggaagg agttagacaa cctgaagtct aggtccctat ttattttttt taatagttat
     1021 gttagtatta agaacgttat ttatatttca aatttttctt ttttttctgt acaaacgcgt
     1081 gtacgcatgt aacattatac tgaaaacctt gcttgagaag gttttgggac gctcggctgc
     1141 caaaccagat gtcaacacag ctacaacgat agcttcgaaa cgtttctact cctcttttac
     1201 tcttccagat cttctcggac tccgcgcatc gccgtaccac ttcaaaacac ccaagcacag
     1261 catactaaat ttcccctctt tcttcctcta gggtgtcgtt aattacccgt actaaaggtt
     1321 tggaaaagaa aaaagagacc gcctcgtttc tttttcttcg tcgaaaaagg caataaaaat
     1381 ttttatcacg tttctttttc ttgaaaattc tttttttgat ttttttctct ttcgatgacc
     1441 tcccatcgat atttaagtta ataaacggcc ttcaatttcc caagtttcag tcccactttt
     1501 cttgctctac tacaactttt tttacctctt gctcattaga aagaaagcat agcaatctaa
     1561 tctaagtttt ctagaaaata tggtgagcaa gggcgaggag gataacatgg ccatcatcaa
     1621 ggagttcatg cgcttcaagg tgcacatgga gggctccgtg gacggccacg agttcgagat
     1681 cgagggcgag ggcgagggcc gcccctacga gggcacccag accgccaagc tgaaggtgac
     1741 caagggtggc cccctgccct tcgcctggga catcctgtcc cctcagttca tgtacggctc
     1801 caaggcctac gtgaagcacc ccgccgacat ccccgactac ttgaagctgt ccttccccga
     1861 gggcttcaag tgggagcgcg tgatgaactt cgaggacggc ggcgtggtga ccgtgaccca
     1921 ggactcctcc ctgcaggacg gcgagttcat ctacaaggtg aagctgcgcg gcaccaactt
     1981 cccctccgac ggccccgtaa tgcagaagaa gactatgggc tgggaggcct cctccgagcg
     2041 gatgtacccc gaggacggcg ccctgaaggg cgagatcaag cagaggctga agctgaagga
     2101 cggcggccac tacgacgctg aggtcaagac cacctacaag gccaagaagc ccgtgcagct
     2161 gcccggcgcc tacaacgtca acatcaagtt ggacatcacc tcccacaacg aggactacac
     2221 catcgtggaa cagtacgaac gcgccgaggg ccgccactcc accggcggca tggacgagct
     2281 gtacaagtaa atcctaactc gaggcgaatt tcttatgatt tatgattttt attattaaat
     2341 aagttataaa aaaaataagt gtatacaaat tttaaagtga ctcttaggtt ttaaaacgaa
     2401 aattcttatt cttgagtaac tctttcctgt aggtcaggtt gctttctcag gtatagcatg
     2461 aggtcgctct tattgaccac acctctaccg gcatgccgag caaatgcctg caaatcgctc
     2521 cccatttcgc tgagcagtta cagagatgtt acgaaccact agtgcactgc agtacataac
     2581 ccctaggtct tcctgagagt gacgaaggct aaggtcactt cttcgtttcc tttattgggg
     2641 tttccgtgta gccttcccct gaatagtgtg ggacgtttta tgagaagccg taagaaatag
     2701 ccgagcggcc gcgtgttaca accaattaac caattctgat tagaaaaact catcgagcat
     2761 caaatgaaac tgcaatttat tcatatcagg attatcaata ccatattttt gaaaaagccg
     2821 tttctgtaat gaaggagaaa actcaccgag gcagttccat aggatggcaa gatcctggta
     2881 tcggtctgcg attccgactc gtccaacatc aatacaacct attaatttcc cctcgtcaaa
     2941 aataaggtta tcaagtgaga aatcaccatg agtgacgact gaatccggtg agaatggcaa
     3001 aagcttatgc atttctttcc agacttgttc aacaggccag ccattacgct cgtcatcaaa
     3061 atcactcgca tcaaccaaac cgttattcat tcgtgattgc gcctgagcga ggcgaaatac
     3121 gcgatcgctg ttaaaaggac aattacaaac aggaatcgaa tgcaaccggc gcaggaacac
     3181 tgccagcgca tcaacaatat tttcacctga atcaggatat tcttctaata cctggaatgc
     3241 tgttttcccg gggatcgcag tggtgagtaa ccatgcatca tcaggagtac ggataaaatg
     3301 cttgatggtc ggaagaggca taaattccgt cagccagttt agtctgacca tctcatctgt
     3361 aacatcattg gcaacgctac ctttgccatg tttcagaaac aactctggcg catcgggctt
     3421 cccatacaat cgatagattg tcgcacctga ttgcccgaca ttatcgcgag cccatttata
     3481 cccatataaa tcagcatcca tgttggaatt taatcgcggc ctggagcaag acgtttcccg
     3541 ttgaatatgg ctcataacac cccttgtatt actgtttatg taagcagaca gttttattgt
     3601 tcatgatgat atatttttat cttgtgcaat gtaacatcag agattttgag acacaacgtg
     3661 gctttgttga ataaatcgaa cttttgctga gttgaaggat cagtcatgac caaaatccct
     3721 taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct
     3781 tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca
     3841 gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc
     3901 agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc
     3961 aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct
     4021 gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag
     4081 gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc
     4141 tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg
     4201 agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag
     4261 cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt
     4321 gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac
     4381 gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg
     4441 ttatcccctg attctgtgga taaccgtgcg gccgccaatg tcaaaagatc ctctcatacc
     4501 atattaagta aattgcctcc atttcttttt cctcgggcag agaaactcgc aggcaacttg
     4561 ctctcgaagt ggtcacgtg
//
