LOCUS       Exported                6554 bp ds-DNA     circular SYN 28-OCT-2022
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6554)
  AUTHORS   Yvonne Nygard
  TITLE     Direct Submission
  JOURNAL   Exported Oct 28, 2022 from SnapGene 4.1.9
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..6554
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    20..44
                     /label=ConS scar
     misc_feature    49..659
                     /label=pPDC1
     CDS             661..1377
                     /codon_start=1
                     /label=ymYPET
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KLLCTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIK
                     ANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSYQSKLSKDPNEKRDHMVLL
                     EFLTAAGITLGMDELYK"
     unsure          1384..1386
                     /label=STOP CODON
     terminator      1393..1648
                     /label=tCYC1
     misc_feature    1653..1677
                     /label=Con1 scar
     misc_feature    1682..2121
                     /label=pADH2
     CDS             2126..2839
                     /label=mTurquoise2
     unsure          2846..2848
                     /label=STOP CODON
     terminator      2855..3110
                     /label=tCYC1
     misc_feature    3119..3139
                     /label=Con2 scar
     promoter        3144..3553
                     /label=pTEFmut8
                     /label=TEF1 promoter mutant 8
     CDS             3555..4265
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVDGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     terminator      4279..4503
                     /label=ScADH1 Terminator
     misc_feature    4508..4532
                     /label=ConE scar
     misc_feature    4552..4571
                     /label=Dummy
     misc_feature    4576..4675
                     /label=Homology Arm 3' X2
     terminator      complement(4688..4714)
                     /label=KanR Terminator
     CDS             complement(4715..5530)
                     /label=KanR
     promoter        complement(5531..5678)
                     /label=KanR Promoter
     rep_origin      complement(5679..6442)
                     /direction=LEFT
                     /label=ColE1
     misc_feature    6455..6554
                     /label=Homology Arm 5' X2
ORIGIN
        1 ccctgaattc gcatctagac tgaactggcc gataattgca gacgaacggt agcttccagt
       61 ttttggtggt tctggcttcc ttcccgattc cgcccgctaa acgcataatt ttgttgcctg
      121 gtggcatttg caaaatgcat aacctatgca tttaaaagat tatgtatgct cttctgactt
      181 ttcgtgtgat gaggctcgtg gaaaaaatga ataatttatg aatttgagaa caattttgtg
      241 ttgttacggt attttactat ggaataatca atcaattgag gattttatgc aaatatcgtt
      301 tgaatatttt tccgaccctt tgagtacttt tcttcataat tgcataatat tgtccgctgc
      361 ccgtttttct gttagacggt gtcttgatct acttgctatc gttcaacacc accttatttt
      421 ctaactattt tttttttagc tcatttgaat cagcttatgg tgatggcaca tttttgcata
      481 aacctagctg tcctcgttga acataggaaa aaaaaatata taaacaaggc tctttcactc
      541 tccttggaat cagatttggg tttgttccct ttattttcat atttcttgtc atattctttt
      601 ctcaattatt atcttctact cataacctca cgcaaaataa cacagtcaaa tcaatcaaat
      661 atggtatcca agggcgaaga attgttcacc ggtgtggtcc caatcttggt cgagttggac
      721 ggtgacgtca acggtcacaa gttctctgtt tccggtgaag gtgaaggaga tgccacctac
      781 ggtaaattga ccttgaagtt gttgtgcacc accggcaagc tgccagttcc atggccaaca
      841 ttggtcacca ctttaggtta tggtttacaa tgttttgcta gatatccaga tcatatgaaa
      901 caacatgatt tcttcaaatc tgctatgcca gaaggttatg ttcaagaaag aactatattt
      961 ttcaaagatg atggtaatta taaaactaga gctgaagtta aatttgaagg agatacttta
     1021 gttaatagaa ttgaattgaa aggtattgat tttaaagaag atggtaatat tttaggtcat
     1081 aaattagaat ataattataa ttctcataat gtttatatta cagctgataa acaaaagaat
     1141 ggtattaagg ctaattttaa aattagacat aatattgaag atggtggtgt tcaattagct
     1201 gatcattatc aacaaaatac tcctattggt gacggtccag ttttattacc tgataatcat
     1261 tatttgtcat atcaatctaa attgtcaaaa gatccaaatg aaaaaagaga tcatatggtt
     1321 ttgttagaat ttttgactgc agctggtatt actttaggta tggatgaatt gtataaagga
     1381 tcctaactcg agatcgatac cgtcgacctc gagtcatgta attagttatg tcacgcttac
     1441 attcacgccc tcctcccaca tccgctctaa ccgaaaagga aggagttaga caacctgaag
     1501 tctaggtccc tatttatttt ttttaatagt tatgttagta ttaagaacgt tatttatatt
     1561 tcaaattttt cttttttttc tgtacaaacg cgtgtacgca tgtaacatta tactgaaaac
     1621 cttgcttgag aaggttttgg gacgctcggc tgccaaacca gatgtcaaca cagctacaac
     1681 gtcctgcctt tctaatcacc attctaatgt tttaattaag ggattttgtc ttcattaacg
     1741 gctttcgctc ataaaaatgt tatgacgttt tgcccgcagg cgggaaacca tccacttcac
     1801 gagactgatc tcctctgccg gaacaccggg catctccaac ttataagttg gagaaataag
     1861 agaatttcag attgagagaa tgaaaaaaaa aaaaaaaaag gcagaggaga gcataaaaat
     1921 ggggttcact ttttggtaaa gctatagcat gcctatcaca tataaataga gtgccagtag
     1981 cgactttttt cacactcgaa atactcttac tactgctctc ttgttgtttt tatcacttct
     2041 tgtttcttct tggtaaatag aatatcaagc tacaaaaagc atacaatcaa ctatcaacta
     2101 ttaactatat cgtaatacac atatggtttc taaaggtgaa gaattattca ctggtgttgt
     2161 cccaattttg gttgaattag atggtgatgt taatggtcac aaattttctg tctccggtga
     2221 aggtgaaggt gatgctactt acggtaaatt gaccttaaaa tttatttgta ctactggtaa
     2281 attgccagtt ccatggccaa ccttagtcac tactttatct tggggtgttc aatgttttgc
     2341 aagataccca gatcatatga aacaacatga ctttttcaag tctgccatgc cagaaggtta
     2401 tgttcaagaa agaactattt ttttcaaaga tgacggtaac tacaagacca gagctgaagt
     2461 caagtttgaa ggtgatacct tagttaatag aatcgaatta aaaggtattg attttaaaga
     2521 agatggtaac attttaggtc acaaattgga atacaattat ttctctgaca atgtttacat
     2581 cactgctgac aaacaaaaga atggtatcaa agctaacttc aaaattagac acaacattga
     2641 agatggtggt gttcaattag ctgaccatta tcaacaaaat actccaattg gtgatggtcc
     2701 agtcttgtta ccagacaacc attacttatc cactcaatct aagttatcca aagatccaaa
     2761 cgaaaagagg gaccacatgg tcttgttaga atttgttact gctgctggta ttaccttggg
     2821 tatggatgaa ttgtacaaag gatcctaact cgagatcgat accgtcgacc tcgagtcatg
     2881 taattagtta tgtcacgctt acattcacgc cctcctccca catccgctct aaccgaaaag
     2941 gaaggagtta gacaacctga agtctaggtc cctatttatt ttttttaata gttatgttag
     3001 tattaagaac gttatttata tttcaaattt ttcttttttt tctgtacaaa cgcgtgtacg
     3061 catgtaacat tatactgaaa accttgcttg agaaggtttt gggacgctcg gctggatgca
     3121 cacactggct taagatgaca acgatagctt cgaaacgttt ctactcctct tttactcttc
     3181 cagatcttct cggactccgc gcatcgccgt accacttcaa aacacccaag cacagcatac
     3241 taaatttccc ctctttcttc ctctagggtg tcgttaatta cccgtactaa aggtttggaa
     3301 aagaaaaaag agaccgcctc gtttcttttt cttcgtcgaa aaaggcaata aaaattttta
     3361 tcacgtttct ttttcttgaa aattcttttt ttgatttttt tctctttcga tgacctccca
     3421 tcgatattta agttaataaa cggccttcaa tttcccaagt ttcagtccca cttttcttgc
     3481 tctactacaa ctttttttac ctcttgctca ttagaaagaa agcatagcaa tctaatctaa
     3541 gttttctaga aaatatggtg agcaagggcg aggaggataa catggccatc atcaaggagt
     3601 tcatgcgctt caaggtgcac atggagggct ccgtggacgg ccacgagttc gagatcgagg
     3661 gcgagggcga gggccgcccc tacgagggca cccagaccgc caagctgaag gtgaccaagg
     3721 gtggccccct gcccttcgcc tgggacatcc tgtcccctca gttcatgtac ggctccaagg
     3781 cctacgtgaa gcaccccgcc gacatccccg actacttgaa gctgtccttc cccgagggct
     3841 tcaagtggga gcgcgtgatg aacttcgagg acggcggcgt ggtgaccgtg acccaggact
     3901 cctccctgca ggacggcgag ttcatctaca aggtgaagct gcgcggcacc aacttcccct
     3961 ccgacggccc cgtaatgcag aagaagacta tgggctggga ggcctcctcc gagcggatgt
     4021 accccgagga cggcgccctg aagggcgaga tcaagcagag gctgaagctg aaggacggcg
     4081 gccactacga cgctgaggtc aagaccacct acaaggccaa gaagcccgtg cagctgcccg
     4141 gcgcctacaa cgtcaacatc aagttggaca tcacctccca caacgaggac tacaccatcg
     4201 tggaacagta cgaacgcgcc gagggccgcc actccaccgg cggcatggac gagctgtaca
     4261 agtaaatcct aactcgaggc gaatttctta tgatttatga tttttattat taaataagtt
     4321 ataaaaaaaa taagtgtata caaattttaa agtgactctt aggttttaaa acgaaaattc
     4381 ttattcttga gtaactcttt cctgtaggtc aggttgcttt ctcaggtata gcatgaggtc
     4441 gctcttattg accacacctc taccggcatg ccgagcaaat gcctgcaaat cgctccccat
     4501 ttcgctgagc agttacagag atgttacgaa ccactagtgc actgcagtac ataaccccta
     4561 ggtcttcctg agagtgacga aggctaaggt cacttcttcg tttcctttat tggggtttcc
     4621 gtgtagcctt cccctgaata gtgtgggacg ttttatgaga agccgtaaga aatagccgag
     4681 cggccgcgtg ttacaaccaa ttaaccaatt ctgattagaa aaactcatcg agcatcaaat
     4741 gaaactgcaa tttattcata tcaggattat caataccata tttttgaaaa agccgtttct
     4801 gtaatgaagg agaaaactca ccgaggcagt tccataggat ggcaagatcc tggtatcggt
     4861 ctgcgattcc gactcgtcca acatcaatac aacctattaa tttcccctcg tcaaaaataa
     4921 ggttatcaag tgagaaatca ccatgagtga cgactgaatc cggtgagaat ggcaaaagct
     4981 tatgcatttc tttccagact tgttcaacag gccagccatt acgctcgtca tcaaaatcac
     5041 tcgcatcaac caaaccgtta ttcattcgtg attgcgcctg agcgaggcga aatacgcgat
     5101 cgctgttaaa aggacaatta caaacaggaa tcgaatgcaa ccggcgcagg aacactgcca
     5161 gcgcatcaac aatattttca cctgaatcag gatattcttc taatacctgg aatgctgttt
     5221 tcccggggat cgcagtggtg agtaaccatg catcatcagg agtacggata aaatgcttga
     5281 tggtcggaag aggcataaat tccgtcagcc agtttagtct gaccatctca tctgtaacat
     5341 cattggcaac gctacctttg ccatgtttca gaaacaactc tggcgcatcg ggcttcccat
     5401 acaatcgata gattgtcgca cctgattgcc cgacattatc gcgagcccat ttatacccat
     5461 ataaatcagc atccatgttg gaatttaatc gcggcctgga gcaagacgtt tcccgttgaa
     5521 tatggctcat aacacccctt gtattactgt ttatgtaagc agacagtttt attgttcatg
     5581 atgatatatt tttatcttgt gcaatgtaac atcagagatt ttgagacaca acgtggcttt
     5641 gttgaataaa tcgaactttt gctgagttga aggatcagtc atgaccaaaa tcccttaacg
     5701 tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga
     5761 tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt
     5821 ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag
     5881 agcgcagata ccaaatactg ttcttctagt gtagccgtag ttaggccacc acttcaagaa
     5941 ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag
     6001 tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca
     6061 gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac
     6121 cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa
     6181 ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc
     6241 agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg
     6301 tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc
     6361 ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc
     6421 ccctgattct gtggataacc gtgcggccgc caatgtcaaa agatcctctc ataccatatt
     6481 aagtaaattg cctccatttc tttttcctcg ggcagagaaa ctcgcaggca acttgctctc
     6541 gaagtggtca cgtg
//
