LOCUS       Exported                5092 bp ds-DNA     circular SYN 31-OCT-2022
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5092)
  AUTHORS   Yvonne Nygard
  TITLE     Direct Submission
  JOURNAL   Exported Oct 31, 2022 from SnapGene 4.1.9
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..5092
                     /organism="recombinant plasmid"
                     /mol_type="other DNA"
     misc_feature    20..44
                     /label=ConS scar
     misc_feature    49..659
                     /label=pPDC1
     CDS             661..1377
                     /codon_start=1
                     /label=ymYPET
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KLLCTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIK
                     ANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSYQSKLSKDPNEKRDHMVLL
                     EFLTAAGITLGMDELYK"
     unsure          1384..1386
                     /label=STOP CODON
     terminator      1393..1648
                     /label=tCYC1
     misc_feature    1653..1677
                     /label=Con1 scar
     promoter        1682..2091
                     /label=pTEFmut8
                     /label=TEF1 promoter mutant 8
     CDS             2093..2803
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVDGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     terminator      2817..3041
                     /label=ScADH1 Terminator
     misc_feature    3046..3070
                     /label=ConE scar
     misc_feature    3090..3109
                     /label=Dummy
     misc_feature    3114..3213
                     /label=Homology Arm 3' X2
     terminator      complement(3226..3252)
                     /label=KanR Terminator
     CDS             complement(3253..4068)
                     /label=KanR
     promoter        complement(4069..4216)
                     /label=KanR Promoter
     rep_origin      complement(4217..4980)
                     /direction=LEFT
                     /label=ColE1
     misc_feature    4993..5092
                     /label=Homology Arm 5' X2
ORIGIN
        1 ccctgaattc gcatctagac tgaactggcc gataattgca gacgaacggt agcttccagt
       61 ttttggtggt tctggcttcc ttcccgattc cgcccgctaa acgcataatt ttgttgcctg
      121 gtggcatttg caaaatgcat aacctatgca tttaaaagat tatgtatgct cttctgactt
      181 ttcgtgtgat gaggctcgtg gaaaaaatga ataatttatg aatttgagaa caattttgtg
      241 ttgttacggt attttactat ggaataatca atcaattgag gattttatgc aaatatcgtt
      301 tgaatatttt tccgaccctt tgagtacttt tcttcataat tgcataatat tgtccgctgc
      361 ccgtttttct gttagacggt gtcttgatct acttgctatc gttcaacacc accttatttt
      421 ctaactattt tttttttagc tcatttgaat cagcttatgg tgatggcaca tttttgcata
      481 aacctagctg tcctcgttga acataggaaa aaaaaatata taaacaaggc tctttcactc
      541 tccttggaat cagatttggg tttgttccct ttattttcat atttcttgtc atattctttt
      601 ctcaattatt atcttctact cataacctca cgcaaaataa cacagtcaaa tcaatcaaat
      661 atggtatcca agggcgaaga attgttcacc ggtgtggtcc caatcttggt cgagttggac
      721 ggtgacgtca acggtcacaa gttctctgtt tccggtgaag gtgaaggaga tgccacctac
      781 ggtaaattga ccttgaagtt gttgtgcacc accggcaagc tgccagttcc atggccaaca
      841 ttggtcacca ctttaggtta tggtttacaa tgttttgcta gatatccaga tcatatgaaa
      901 caacatgatt tcttcaaatc tgctatgcca gaaggttatg ttcaagaaag aactatattt
      961 ttcaaagatg atggtaatta taaaactaga gctgaagtta aatttgaagg agatacttta
     1021 gttaatagaa ttgaattgaa aggtattgat tttaaagaag atggtaatat tttaggtcat
     1081 aaattagaat ataattataa ttctcataat gtttatatta cagctgataa acaaaagaat
     1141 ggtattaagg ctaattttaa aattagacat aatattgaag atggtggtgt tcaattagct
     1201 gatcattatc aacaaaatac tcctattggt gacggtccag ttttattacc tgataatcat
     1261 tatttgtcat atcaatctaa attgtcaaaa gatccaaatg aaaaaagaga tcatatggtt
     1321 ttgttagaat ttttgactgc agctggtatt actttaggta tggatgaatt gtataaagga
     1381 tcctaactcg agatcgatac cgtcgacctc gagtcatgta attagttatg tcacgcttac
     1441 attcacgccc tcctcccaca tccgctctaa ccgaaaagga aggagttaga caacctgaag
     1501 tctaggtccc tatttatttt ttttaatagt tatgttagta ttaagaacgt tatttatatt
     1561 tcaaattttt cttttttttc tgtacaaacg cgtgtacgca tgtaacatta tactgaaaac
     1621 cttgcttgag aaggttttgg gacgctcggc tgccaaacca gatgtcaaca cagctacaac
     1681 gatagcttcg aaacgtttct actcctcttt tactcttcca gatcttctcg gactccgcgc
     1741 atcgccgtac cacttcaaaa cacccaagca cagcatacta aatttcccct ctttcttcct
     1801 ctagggtgtc gttaattacc cgtactaaag gtttggaaaa gaaaaaagag accgcctcgt
     1861 ttctttttct tcgtcgaaaa aggcaataaa aatttttatc acgtttcttt ttcttgaaaa
     1921 ttcttttttt gatttttttc tctttcgatg acctcccatc gatatttaag ttaataaacg
     1981 gccttcaatt tcccaagttt cagtcccact tttcttgctc tactacaact ttttttacct
     2041 cttgctcatt agaaagaaag catagcaatc taatctaagt tttctagaaa atatggtgag
     2101 caagggcgag gaggataaca tggccatcat caaggagttc atgcgcttca aggtgcacat
     2161 ggagggctcc gtggacggcc acgagttcga gatcgagggc gagggcgagg gccgccccta
     2221 cgagggcacc cagaccgcca agctgaaggt gaccaagggt ggccccctgc ccttcgcctg
     2281 ggacatcctg tcccctcagt tcatgtacgg ctccaaggcc tacgtgaagc accccgccga
     2341 catccccgac tacttgaagc tgtccttccc cgagggcttc aagtgggagc gcgtgatgaa
     2401 cttcgaggac ggcggcgtgg tgaccgtgac ccaggactcc tccctgcagg acggcgagtt
     2461 catctacaag gtgaagctgc gcggcaccaa cttcccctcc gacggccccg taatgcagaa
     2521 gaagactatg ggctgggagg cctcctccga gcggatgtac cccgaggacg gcgccctgaa
     2581 gggcgagatc aagcagaggc tgaagctgaa ggacggcggc cactacgacg ctgaggtcaa
     2641 gaccacctac aaggccaaga agcccgtgca gctgcccggc gcctacaacg tcaacatcaa
     2701 gttggacatc acctcccaca acgaggacta caccatcgtg gaacagtacg aacgcgccga
     2761 gggccgccac tccaccggcg gcatggacga gctgtacaag taaatcctaa ctcgaggcga
     2821 atttcttatg atttatgatt tttattatta aataagttat aaaaaaaata agtgtataca
     2881 aattttaaag tgactcttag gttttaaaac gaaaattctt attcttgagt aactctttcc
     2941 tgtaggtcag gttgctttct caggtatagc atgaggtcgc tcttattgac cacacctcta
     3001 ccggcatgcc gagcaaatgc ctgcaaatcg ctccccattt cgctgagcag ttacagagat
     3061 gttacgaacc actagtgcac tgcagtacat aacccctagg tcttcctgag agtgacgaag
     3121 gctaaggtca cttcttcgtt tcctttattg gggtttccgt gtagccttcc cctgaatagt
     3181 gtgggacgtt ttatgagaag ccgtaagaaa tagccgagcg gccgcgtgtt acaaccaatt
     3241 aaccaattct gattagaaaa actcatcgag catcaaatga aactgcaatt tattcatatc
     3301 aggattatca ataccatatt tttgaaaaag ccgtttctgt aatgaaggag aaaactcacc
     3361 gaggcagttc cataggatgg caagatcctg gtatcggtct gcgattccga ctcgtccaac
     3421 atcaatacaa cctattaatt tcccctcgtc aaaaataagg ttatcaagtg agaaatcacc
     3481 atgagtgacg actgaatccg gtgagaatgg caaaagctta tgcatttctt tccagacttg
     3541 ttcaacaggc cagccattac gctcgtcatc aaaatcactc gcatcaacca aaccgttatt
     3601 cattcgtgat tgcgcctgag cgaggcgaaa tacgcgatcg ctgttaaaag gacaattaca
     3661 aacaggaatc gaatgcaacc ggcgcaggaa cactgccagc gcatcaacaa tattttcacc
     3721 tgaatcagga tattcttcta atacctggaa tgctgttttc ccggggatcg cagtggtgag
     3781 taaccatgca tcatcaggag tacggataaa atgcttgatg gtcggaagag gcataaattc
     3841 cgtcagccag tttagtctga ccatctcatc tgtaacatca ttggcaacgc tacctttgcc
     3901 atgtttcaga aacaactctg gcgcatcggg cttcccatac aatcgataga ttgtcgcacc
     3961 tgattgcccg acattatcgc gagcccattt atacccatat aaatcagcat ccatgttgga
     4021 atttaatcgc ggcctggagc aagacgtttc ccgttgaata tggctcataa caccccttgt
     4081 attactgttt atgtaagcag acagttttat tgttcatgat gatatatttt tatcttgtgc
     4141 aatgtaacat cagagatttt gagacacaac gtggctttgt tgaataaatc gaacttttgc
     4201 tgagttgaag gatcagtcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg
     4261 tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc
     4321 tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag
     4381 ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtt
     4441 cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac
     4501 ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc
     4561 gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt
     4621 tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt
     4681 gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc
     4741 ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt
     4801 tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca
     4861 ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt
     4921 tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt
     4981 gcggccgcca atgtcaaaag atcctctcat accatattaa gtaaattgcc tccatttctt
     5041 tttcctcggg cagagaaact cgcaggcaac ttgctctcga agtggtcacg tg
//
