LOCUS       Box9_C4                 6853 bp    DNA     circular SYN 30-OCT-2022
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
COMMENT     Annotated with pLannotate v1.2.0
FEATURES             Location/Qualifiers
     rep_origin      complement(406..834)
                     /note="pLannotate"
                     /label="f1 ori"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="rep_origin"
     polyA_signal    complement(4790..4924)
                     /note="pLannotate"
                     /label="SV40 poly(A) signal"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="polyA_signal"
     promoter        complement(249..353)
                     /note="pLannotate"
                     /label="AmpR promoter"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
     promoter        912..1889
                     /note="pLannotate"
                     /label="CMV IE94 promoter"
                     /database="snapgene"
                     /identity="99.9"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
     CDS             complement(join(6241..6853,1..248))
                     /note="pLannotate"
                     /label="AmpR"
                     /database="snapgene"
                     /identity="99.8"
                     /match_length="100.0"
                     /fragment="False"
                     /other="CDS"
     rep_origin      complement(5482..6070)
                     /note="pLannotate"
                     /label="ori"
                     /database="snapgene"
                     /identity="99.8"
                     /match_length="100.0"
                     /fragment="False"
                     /other="rep_origin"
     CDS             4170..4757
                     /note="pLannotate"
                     /label="FokI cleavage domain"
                     /database="snapgene"
                     /identity="99.2"
                     /match_length="100.0"
                     /fragment="False"
                     /other="CDS"
     protein_bind    complement(5173..5194)
                     /note="pLannotate"
                     /label="CAP binding site"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="protein_bind"
     promoter        1924..1942
                     /note="pLannotate"
                     /label="SP6 promoter"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
     promoter        complement(5041..5059)
                     /note="pLannotate"
                     /label="T3 promoter"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
     protein_bind    complement(5104..5120)
                     /note="pLannotate"
                     /label="lac operator"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="protein_bind"
     CDS             2007..2072
                     /note="pLannotate"
                     /label="3xFLAG"
                     /database="snapgene"
                     /identity="98.5"
                     /match_length="100.0"
                     /fragment="False"
                     /other="CDS"
     CDS             2079..2588
                     /note="pLannotate"
                     /label="avrXa10 (fragment)"
                     /database="swissprot"
                     /identity="79.4"
                     /match_length="15.4"
                     /fragment="True"
                     /other="CDS"
     promoter        complement(5128..5158)
                     /note="pLannotate"
                     /label="lac promoter"
                     /database="snapgene"
                     /identity="96.8"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
     CDS             complement(839..905)
                     /note="pLannotate"
                     /label="lacZα (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="38.5"
                     /fragment="True"
                     /other="CDS"
     promoter        complement(4768..4785)
                     /note="pLannotate"
                     /label="T7 promoter (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="94.7"
                     /fragment="True"
                     /other="promoter"
     CDS             complement(5207..5299)
                     /note="pLannotate"
                     /label="lacI (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="8.6"
                     /fragment="True"
                     /other="CDS"
ORIGIN
        1 tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa
       61 cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa
      121 cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga
      181 gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga
      241 atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg
      301 agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt
      361 ccccgaaaag tgccacctaa attgtaagcg ttaatatttt gttaaaattc gcgttaaatt
      421 tttgttaaat cagctcattt tttaaccaat aggccgaaat cggcaaaatc ccttataaat
      481 caaaagaata gaccgagata gggttgagtg ttgttccagt ttggaacaag agtccactat
      541 taaagaacgt ggactccaac gtcaaagggc gaaaaaccgt ctatcagggc gatggcccac
      601 tacgtgaacc atcaccctaa tcaagttttt tggggtcgag gtgccgtaaa gcactaaatc
      661 ggaaccctaa agggagcccc cgatttagag cttgacgggg aaagccggcg aacgtggcga
      721 gaaaggaagg gaagaaagcg aaaggagcgg gcgctagggc gctggcaagt gtagcggtca
      781 cgctgcgcgt aaccaccaca cccgccgcgc ttaatgcgcc gctacagggc gcgtcccatt
      841 cgccattcag gctgcgcaac tgttgggaag ggcgatcggt gcgggcctct tcgctattac
      901 gccagtcgac cgccaattca atatggcgta tatggactca tgccaattca atatggtgga
      961 tctggacctg tgccaattca atatggcgta tatggactcg tgccaattca atatggtgga
     1021 tctggacccc agccaattca atatggcgga cttggcacca tgccaattca atatggcgga
     1081 cctggcactg tgccaactgg ggaggggtct acttggcacg gtgccaagtt tgaggagggg
     1141 tcttggccct gtgccaagtc cgccatattg aattggcatg gtgccaataa tggcggccat
     1201 attggctata tgccaggatc aatatatagg caatatccaa tatggcccta tgccaatatg
     1261 gctattggcc aggttcaata ctatgtattg gccctatgcc atatagtatt ccatatatgg
     1321 gttttcctat tgacgtagat agcccctccc aatgggcggt cccatatacc atatatgggg
     1381 cttcctaata ccgcccatag ccactccccc attgacgtca atggtctcta tatatggtct
     1441 ttcctattga cgtcatatgg gcggtcctat tgacgtatat ggcgcctccc ccattgacgt
     1501 caattacggt aaatggcccg cctggctcaa tgcccattga cgtcaatagg accacccacc
     1561 attgacgtca atgggatggc tcattgccca ttcatatccg ttctcacgcc ccctattgac
     1621 gtcaatgacg gtaaatggcc cacttggcag tacatcaata tctattaata gtaacttggc
     1681 aagtacatta ctattggaag tacgccaggg tacattggca gtactcccat tgacgtcaat
     1741 ggcggtaaat ggcccgcgat ggctgccaag tacatcccca ttgacgtcaa tggggagggg
     1801 caatgacgca aatgggcgtt ccattgacgt aaatgggcgg taggcgtgcc taatgggagg
     1861 tctatataag caatgctcgt ttagggaacc gccattctgc ctggggacgt cggagcaagc
     1921 ttgatttagg tgacactata gaatacaagc tacttgttct ttttgcagga tctgccacca
     1981 tggctccaaa gaagaagcgt aaggtagact acaaagacca tgacggtgat tataaagatc
     2041 atgacatcga ttacaaggat gacgatgaca agggtaccgt ggatctacgc acgctcggct
     2101 acagtcagca gcagcaagag aagatcaaac cgaaggtgcg ttcgacagtg gcgcagcacc
     2161 acgaggcact ggtgggccat gggtttacac acgcgcacat cgttgcgctc agccaacacc
     2221 cggcagcgtt agggaccgtc gctgtcacgt atcagcacat aatcacggcg ttgccagagg
     2281 cgacacacga agacatcgtt ggcgtcggca aacagtggtc cggcgcacgc gccctggagg
     2341 ccttgctcac ggatgcgggg gagttgagag gtccgccgtt acagttggac acaggccaac
     2401 ttgtgaagat tgcaaaacgt ggcggcgtga ccgcaatgga ggcagtgcat gcatcgcgca
     2461 atgcactgac gggtgccccc ctgaacctga ccccggacca agtggtggct atcgccagca
     2521 acaatggcgg caagcaagcg ctcgaaacgg tgcagcggct gttgccggtg ctgtgccagg
     2581 accatggcct gactccggac caagtggtgg ctatcgccag ccacgatggc ggcaagcaag
     2641 cgctcgaaac ggtgcagcgg ctgttgccgg tgctgtgcca ggaccatggc ctgaccccgg
     2701 accaagtggt ggctatcgcc agcaacggtg gcggcaagca agcgctcgaa acggtgcagc
     2761 ggctgttgcc ggtgctgtgc caggaccatg gcctgacccc ggaccaagtg gtggctatcg
     2821 ccagcaacgg tggcggcaag caagcgctcg aaacggtgca gcggctgttg ccggtgctgt
     2881 gccaggacca tggcctgacc ccggaccaag tggtggctat cgccagcaac ggtggcggca
     2941 agcaagcgct cgaaacggtg cagcggctgt tgccggtgct gtgccaggac catggcctga
     3001 ctccggacca agtggtggct atcgccagcc acgatggcgg caagcaagcg ctcgaaacgg
     3061 tgcagcggct gttgccggtg ctgtgccagg accatggcct gaccccggac caagtggtgg
     3121 ctatcgccag caacattggc ggcaagcaag cgctcgaaac ggtgcagcgg ctgttgccgg
     3181 tgctgtgcca ggaccatggc ctgaccccgg accaagtggt ggctatcgcc agcaacggtg
     3241 gcggcaagca agcgctcgaa acggtgcagc ggctgttgcc ggtgctgtgc caggaccatg
     3301 gcctgacccc ggaccaagtg gtggctatcg ccagcaacgg tggcggcaag caagcgctcg
     3361 aaacggtgca gcggctgttg ccggtgctgt gccaggacca tggcctgacc ccggaccaag
     3421 tggtggctat cgccagcaac attggcggca agcaagcgct cgaaacggtg cagcggctgt
     3481 tgccggtgct gtgccaggac catggcctga ccccggacca agtggtggct atcgccagca
     3541 acattggcgg caagcaagcg ctcgaaacgg tgcagcggct gttgccggtg ctgtgccagg
     3601 accatggcct gactccggac caagtggtgg ctatcgccag ccacgatggc ggcaagcaag
     3661 cgctcgaaac ggtgcagcgg ctgttgccgg tgctgtgcca ggaccatggc ctgaccccgg
     3721 accaagtggt ggctatcgcc agcaacggtg gcggcaagca agcgctcgaa acggtgcagc
     3781 ggctgttgcc ggtgctgtgc caggaccatg gcctgactcc ggaccaagtg gtggctatcg
     3841 ccagccacga tggcggcaag caagcgctcg aaacggtgca gcggctgttg ccggtgctgt
     3901 gccaggacca tggcctgacc ccggaccaag tggtggctat cgccagccac gatggcggca
     3961 agcaagcgct cgaaagcatt gtggcccagc tgagccggcc tgatccggcg ttggccgcgt
     4021 tgaccaacga ccacctcgtc gccttggcct gcctcggcgg acgtcctgcc atggatgcag
     4081 tgaaaaaggg attgccgcac gcgccggaat tgatcagaag agtcaatcgc cgtattggcg
     4141 aacgcacgtc ccatcgcgtt gccggatccc aactagtcaa aagtgaactg gaggagaaga
     4201 aatctgaact tcgtcataaa ttgaaatatg tgcctcatga atatattgaa ttaattgaaa
     4261 ttgccagaaa ttccactcag gatagaattc ttgaaatgaa ggtaatggaa ttttttatga
     4321 aagtttatgg atatagaggt aaacatttgg gtggatcaag gaaaccggac ggagcaattt
     4381 atactgtcgg atctcctatt gattacggtg tgatcgtgga tactaaagct tatagcggag
     4441 gttataatct gccaattggc caagcagatg aaatgcaaga ctatgtcgaa gaaaatcaaa
     4501 cacgaaacaa acatatcaac cctaatgaat ggtggaaagt ctatccatct tctgtaacgg
     4561 aatttaagtt tttatttgtg agtggtcact ttaaaggaaa ctacaaagct cagcttacac
     4621 gattaaatca tatcactaat tgtaatggag ctgttcttag tgtagaagag cttttaattg
     4681 gtggagaaat gattaaagcc ggcacattaa ccttagagga agtgcgccgg aaatttaata
     4741 acggcgagat aaacttttaa tctagaacta tagtgagtcg tattacgtag atccagacat
     4801 gataagatac attgatgagt ttggacaaac cacaactaga atgcagtgaa aaaaatgctt
     4861 tatttgtgaa atttgtgatg ctattgcttt atttgtaacc attataagct gcaataaaca
     4921 agttaacaac aacaattgca ttcattttat gtttcaggtt cagggggagg tgtgggaggt
     4981 tttttaattc gcggccgcgg cgccaatgca ttgggcccgg tacgtaccca gcttttgttc
     5041 cctttagtga gggttaattg cgcgcttggc gtaatcatgg tcatagctgt ttcctgtgtg
     5101 aaattgttat ccgctcacaa ttccacacaa catacgagcc gggagcataa agtgtaaagc
     5161 ctggggtgcc taatgagtga gctaactcac attaattgcg ttgcgctcac tgcccgcttt
     5221 ccagtcggga aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg cggggagagg
     5281 cggtttgcgt attgggcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt
     5341 tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc
     5401 aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa
     5461 aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa
     5521 tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc
     5581 ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc
     5641 cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag
     5701 ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga
     5761 ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc
     5821 gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac
     5881 agagttcttg aagtggtggc ctaactacgg ctacactaga agaacagtat ttggtatctg
     5941 cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca
     6001 aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa
     6061 aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa
     6121 ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt
     6181 aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag
     6241 ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat
     6301 agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc
     6361 cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa
     6421 ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca
     6481 gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa
     6541 cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt
     6601 cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc
     6661 ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact
     6721 catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc
     6781 tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg
     6841 ctcttgcccg gcg
//
