LOCUS       pRRL.SIN.EF1A.JAG1-F1.GS.CAR-2G       10510 bp    DNA     circular SYN  3-NOV-2022
DEFINITION  3rd generation lentiviral backbone.
ACCESSION   .
VERSION     .
KEYWORDS    S7_pRRLSIN; EF1a; CRE; IRES; Puro; WPRE; v2.
SOURCE      synthetic DNA construct.
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9606)
  TITLE     Trono Lab Misc Plasmids
REFERENCE   2  (bases 1 to 9606)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Tuesday, Jun 20, 2017 from SnapGene Console 4.0.0a8 
            http://www.snapgene.com
REFERENCE   3  (bases 1 to 9606)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported sexta-feira, 1 de fev de 2019 from SnapGene 1.1.3
            http://www.snapgene.com
COMMENT     This file is created by Vector NTI
            http://www.invitrogen.com/
COMMENT     ORIGDB|GenBank
COMMENT     VNTDATE|-14641155|
COMMENT     VNTDBDATE|-14641155|
COMMENT     LSOWNER|
COMMENT     VNTNAME|pRRL.SIN.EF1A.JAG1-F1.GS.CAR-2G|
COMMENT     VNTAUTHORNAME|Ana Filipa Rodrigues|
COMMENT     VNTAUTHOREML|afrodrigues@ibet.pt|
COMMENT     VNTOAUTHORNAME|UNKNOWN|
COMMENT     Vector_NTI_Display_Data_(Do_Not_Edit!)
COMMENT     (SXF 
COMMENT      (CGexDoc "pRRL.SIN.EF1A.JAG1-F1.GS.CAR-2G" 0 10510 
COMMENT       (CDBMol 0 0 1 1 1 0 0 1 0 "" "" 0 0 0 0 (CObList) (CObList) (CObList) 
COMMENT        (CObList) -1 "") 
COMMENT       (CDocSetData 1 1 0 0 0 0 "MAIN" 1 1 1 1 0 0 1 1 0 1 10 10 4294967295 50 0 
COMMENT        1 0 (CHomObj 0 0 0 3 75) (CWordArray) (CWordArray) 
COMMENT        (CStringList "EcoRI" "BsiWI" "NotI" "NheI" "SalI" "SpeI") 
COMMENT        (CStringList "atg" "gtg") (CStringList "taa" "tga" "tag") (CObList) 1 
COMMENT        "{(0,1),2}" 0 0 "" 0 4294967295 0 0 0 0 0 0 0 "MAIN" 0 0 30 0 
COMMENT        (CProteinMotifSearchObject 70 20 1 1 1 1 0 0 1 0 0 0 0 0)) 
COMMENT       (CMolPar 0 0 0 0 4294967295 1 10510 0 0 0 0 0 0 0 0) (CStringList) 
COMMENT       (CStringList) (CObList) (COAPar 25 250 50 0 6 4 3 7) 
COMMENT       (COAPar 25 250 50 0 6 4 3 7) (COAPar 25 250 50 0 6 4 3 7) 
COMMENT       (CObList #0=(CRSite (CStringList) "EcoRI" "gaattc" 2 0 1 3140 0 0 "") 
COMMENT        #1=(CRSite (CStringList) "BsiWI" "cgtacg" 2 0 1 4613 0 0 "") 
COMMENT        #2=(CRSite (CStringList) "NotI" "gcggccgc" 3 0 1 3933 0 0 "") 
COMMENT        #3=(CRSite (CStringList) "NheI" "gctagc" 2 0 0 0 "") 
COMMENT        #4=(CRSite (CStringList) "SalI" "gtcgac" 2 0 1 6349 0 0 "") 
COMMENT        #5=(CRSite (CStringList) "SpeI" "actagt" 2 0 1 5248 0 0 "")) 
COMMENT       (CObList 
COMMENT        #6=(CFSignal (CObList) "RSV promoter" 30 0 0 1 227 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "1..227") 
COMMENT        #7=(CFSignal (CObList) "5' LTR (RU5)" 19 0 0 228 408 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "228..408") 
COMMENT        #8=(CFSignal (CObList) "SL123" 21 0 0 410 563 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "410..563") 
COMMENT        #9=(CFSignal (CObList) "HIV-1 Psi" 21 0 0 455 580 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "455..580") 
COMMENT        #10=(CFSignal (CObList) "SL4 (Gag)" 4 0 0 564 927 0 (CStringList) 
COMMENT             (CStringList "/codon_start=1") 1 1 1 1 "564..927") 
COMMENT        #11=(CFSignal (CObList) "RRE" 21 0 0 1073 1306 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "1073..1306") 
COMMENT        #12=(CFSignal (CObList) "cPPT/CTS" 21 0 0 1802 1919 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "1802..1919") 
COMMENT        #13=(CFSignal (CObList) "EF-1a" 30 0 0 1944 3122 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "1944..3122") 
COMMENT        #14=(CFSignal (CObList) "Spacer for EMCV IRES" 21 0 0 4618 4685 0 
COMMENT             (CStringList) (CStringList) 1 1 1 1 "4618..4685") 
COMMENT        #15=(CFSignal (CObList) "EMCV IRES" 21 0 0 4686 5246 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "4686..5246") 
COMMENT        #16=(CFSignal (CObList) "WPRE" 21 0 0 6354 6942 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "6354..6942") 
COMMENT        #17=(CFSignal (CObList) "Factor Xa site" 4 0 1 6825 6836 0 (CStringList) 
COMMENT             (CStringList "/codon_start=1" 
COMMENT              "/product=\"Factor Xa recognition and cleavage site\"") 1 1 1 1 
COMMENT             "complement(6825..6836)") 
COMMENT        #18=(CFSignal (CObList) "3' LTR (Delta-U3RU5)" 19 0 0 7029 7262 0 
COMMENT             (CStringList) (CStringList) 1 1 1 1 "7029..7262") 
COMMENT        #19=(CFSignal (CObList) "SV40 poly(A) signal" 25 0 0 7334 7455 0 
COMMENT             (CStringList) (CStringList) 1 1 1 1 "7334..7455") 
COMMENT        #20=(CFSignal (CObList) "SV40 ori" 33 0 0 7495 7630 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "7495..7630") 
COMMENT        #21=(CFSignal (CObList) "T7 promoter" 30 0 1 7651 7669 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "complement(7651..7669)") 
COMMENT        #22=(CFSignal (CObList) "f1 ori" 33 0 0 7837 8292 0 (CStringList) 
COMMENT             (CStringList "/direction=RIGHT") 1 1 1 1 "7837..8292") 
COMMENT        #23=(CFSignal (CObList) "AmpR promoter" 30 0 0 8318 8422 0 (CStringList) 
COMMENT             (CStringList "/gene=\"bla\"") 1 1 1 1 "8318..8422") 
COMMENT        #24=(CFSignal (CObList) "AmpR" 4 0 0 8423 9283 0 (CStringList) 
COMMENT             (CStringList "/codon_start=1" "/gene=\"bla\"" 
COMMENT              "/product=\"beta-lactamase\"") 1 1 1 1 "8423..9283") 
COMMENT        #25=(CFSignal (CObList) "ori" 33 0 0 9454 10042 0 (CStringList) 
COMMENT             (CStringList "/direction=RIGHT") 1 1 1 1 "9454..10042") 
COMMENT        #26=(CFSignal (CObList) "CAP binding site" 31 0 0 10330 10351 0 
COMMENT             (CStringList) 
COMMENT             (CStringList 
COMMENT              "/bound_moiety=\"E. coli catabolite activator protein\"") 1 1 1 1 
COMMENT             "10330..10351") 
COMMENT        #27=(CFSignal (CObList) "lac promoter" 30 0 0 10366 10396 0 
COMMENT             (CStringList) (CStringList) 1 1 1 1 "10366..10396") 
COMMENT        #28=(CFSignal (CObList) "lac operator" 31 0 0 10404 10420 0 
COMMENT             (CStringList) 
COMMENT             (CStringList "/bound_moiety=\"lac repressor encoded by lacI\"") 1 1 
COMMENT             1 1 "10404..10420") 
COMMENT        #29=(CFSignal (CObList) "Promoter_P_1" 30 0 0 10465 1 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "10465..1") 
COMMENT        #30=(CFSignal (CObList) "T3 promoter" 30 0 0 10465 10483 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "10465..10483") 
COMMENT        #31=(CFSignal (CObList) "GFP-ZEO" 21 0 0 5253 6347 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "5253..6347") 
COMMENT        #32=(CFSignal (CObList) "AAA linker (also NotI)" 21 0 0 3931 3939 0 
COMMENT             (CStringList) (CStringList) 1 1 1 1 "3931..3939") 
COMMENT        #33=(CFSignal (CObList) "CD8A" 21 0 0 3940 4146 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "3940..4146") 
COMMENT        #34=(CFSignal (CObList) "TM region" 21 0 0 4075 4146 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "4075..4146") 
COMMENT        #35=(CFSignal (CObList) "41BB" 21 0 0 4147 4272 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "4147..4272") 
COMMENT        #36=(CFSignal (CObList) "CD3Z" 21 0 0 4273 4611 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "4273..4611") 
COMMENT        #37=(CFSignal (CObList) "GMCSF Leader Signal" 21 0 0 3151 3216 0 
COMMENT             (CStringList) (CStringList) 1 1 1 1 "3151..3216") 
COMMENT        #38=(CFSignal (CObList) "(GGGGS)3 linker" 21 0 0 3565 3609 0 
COMMENT             (CStringList) (CStringList) 1 1 1 1 "3565..3609") 
COMMENT        #39=(CFSignal (CObList) "J1.F1 VH" 21 0 0 3217 3564 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "3217..3564") 
COMMENT        #40=(CFSignal (CObList) "J1.F1 VL" 21 0 0 3610 3930 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "3610..3930")) (CObList) (CObList) (CObList) 
COMMENT       (CObList) (CObList) (CObList) 
COMMENT       (CTextView 0 
COMMENT        #41=(CGroupPar (CParagraph 0 (0 0) 1 2 0 0 180) 
COMMENT             (CObjectList 
COMMENT              #42=(CRefLinePar 
COMMENT                   (CLinePar (CParagraph 0 (0 0) 0 2 0 0 233) 
COMMENT                    "pRRL.SIN.EF1A.JAG1-F1.GS.CAR-2G" 2) 5 "" 0 4) 
COMMENT              #43=(CFolderPar 
COMMENT                   (CGroupPar (CParagraph 1 (0 0) 1 1 0 0 178) 
COMMENT                    (CObjectList 
COMMENT                     #44=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "DNA 'pRRL.SIN.EF1A.JAG1-F1.GS.CAR-2G'" 1) 
COMMENT                     #45=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "3rd generation lentiviral backbone." 1) 
COMMENT                     #46=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Foreign object. Author: Ana Filipa Rodrigues. Original author: UNKNOWN" 
COMMENT                          1) 
COMMENT                     #47=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Created: 11/03/22 09:30" 1) 
COMMENT                     #48=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Last Modified: 11/03/22 09:30" 1) 
COMMENT                     #49=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "length: 10510 bp" 1) 
COMMENT                     #50=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "storage type: Basic" 1) 
COMMENT                     #51=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "form: Circular" 1))) "General Description") 
COMMENT              #52=(CFolderPar 
COMMENT                   (CGroupPar (CParagraph 2 (0 0) 1 1 0 0 178) 
COMMENT                    (CObjectList 
COMMENT                     #53=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Keywords: S7_pRRLSIN; EF1a; CRE; IRES; Puro; WPRE; v2" 
COMMENT                          1) 
COMMENT                     #54=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Original Source Database: GenBank" 1) 
COMMENT                     #55=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Division: SYN" 1) 
COMMENT                     #56=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Modification Date in the Original DB: 3-NOV-2022" 1) 
COMMENT                     #57=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Original Accession Number(s): <a href=\"ncbilink:dnarna/.\">.</a>" 
COMMENT                          1) 
COMMENT                     #58=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Version in the Original DB: ." 1) 
COMMENT                     #59=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Sequence Source: synthetic DNA construct." 1) 
COMMENT                     #60=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Organism(s): <a href=\"ncbilink:taxonomy/synthetic+DNA+construct\">synthetic DNA construct(synthetic DNA construct)</a>" 
COMMENT                          1) 
COMMENT                     #61=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Associated Genes:  bla" 1))) "Standard Fields") 
COMMENT              #62=(CFolderPar 
COMMENT                   (CGroupPar (CParagraph 3 (0 0) 1 1 0 0 178) 
COMMENT                    (CObjectList 
COMMENT                     #63=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "REFERENCE   1  (bases 1 to 9606)" 1) 
COMMENT                     #64=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "  TITLE     Trono Lab Misc Plasmids" 1) 
COMMENT                     #65=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "REFERENCE   2  (bases 1 to 9606)" 1) 
COMMENT                     #66=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "  AUTHORS   ." 1) 
COMMENT                     #67=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "  TITLE     Direct Submission" 1) 
COMMENT                     #68=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "  JOURNAL   Exported Tuesday, Jun 20, 2017 from SnapGene Console 4.0.0a8 " 
COMMENT                          1) 
COMMENT                     #69=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "            http://www.snapgene.com" 1) 
COMMENT                     #70=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "REFERENCE   3  (bases 1 to 9606)" 1) 
COMMENT                     #71=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "  AUTHORS   ." 1) 
COMMENT                     #72=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "  TITLE     Direct Submission" 1) 
COMMENT                     #73=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "  JOURNAL   Exported sexta-feira, 1 de fev de 2019 from SnapGene 1.1.3" 
COMMENT                          1) 
COMMENT                     #74=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "            http://www.snapgene.com" 1))) 
COMMENT                   "References") 
COMMENT              #75=(CFolderPar 
COMMENT                   (CGroupPar (CParagraph 4 (0 0) 1 1 0 0 178) 
COMMENT                    (CObjectList 
COMMENT                     #76=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Ana Filipa Rodrigues" 1) 
COMMENT                     #77=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "EMAIL: <a href=\"mailto:afrodrigues@ibet.pt\">afrodrigues@ibet.pt</a>" 
COMMENT                          1))) "Author") 
COMMENT              #78=(CRefLinePar 
COMMENT                   (CLinePar (CParagraph 0 (0 0) 0 2 0 0 233) "Comments" 2) 1 "" 
COMMENT                   0 0) 
COMMENT              #79=(CFolderPar 
COMMENT                   (CGroupPar (CParagraph 8 (0 0) 1 2 0 0 178) (CObjectList)) 
COMMENT                   "Annotations") 
COMMENT              #80=(CFolderPar 
COMMENT                   (CGroupPar (CParagraph 12 (6 0) 1 2 0 0 178) 
COMMENT                    (CObjectList 
COMMENT                     #81=(CFolderPar 
COMMENT                          (CGroupPar (CParagraph 4 (7 4 0) 1 1 1 0 178) 
COMMENT                           (CObjectList 
COMMENT                            #82=(CFolderPar 
COMMENT                                 (CGroupPar 
COMMENT                                  (CParagraph 564 (3 #10# 0) 1 2 2 0 327) 
COMMENT                                  (CObjectList 
COMMENT                                   #83=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 564   End: 927 " 1) 
COMMENT                                   #84=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Original Location Description:" 1) 
COMMENT                                   #85=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   564..927" 1) 
COMMENT                                   #86=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Qualifiers:" 1) 
COMMENT                                   #87=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   /codon_start=1" 1))) "SL4 (Gag)") 
COMMENT                            #88=(CFolderPar 
COMMENT                                 (CGroupPar 
COMMENT                                  (CParagraph 6825 (3 #17# 0) 1 2 2 0 327) 
COMMENT                                  (CObjectList 
COMMENT                                   #89=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 6825  End: 6836 (Complementary)" 
COMMENT                                        1) 
COMMENT                                   #90=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Original Location Description:" 1) 
COMMENT                                   #91=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   complement(6825..6836)" 1) 
COMMENT                                   #92=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Qualifiers:" 1) 
COMMENT                                   #93=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   /codon_start=1" 1) 
COMMENT                                   #94=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   /product=\"Factor Xa recognition and cleavage site\"" 
COMMENT                                        1))) "Factor Xa site") 
COMMENT                            #95=(CFolderPar 
COMMENT                                 (CGroupPar 
COMMENT                                  (CParagraph 8423 (3 #24# 0) 1 2 2 0 327) 
COMMENT                                  (CObjectList 
COMMENT                                   #96=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 8423  End: 9283" 1) 
COMMENT                                   #97=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Original Location Description:" 1) 
COMMENT                                   #98=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   8423..9283" 1) 
COMMENT                                   #99=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Qualifiers:" 1) 
COMMENT                                   #100=(CLinePar 
COMMENT                                         (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                         "   /codon_start=1" 1) 
COMMENT                                   #101=(CLinePar 
COMMENT                                         (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                         "   /gene=\"bla\"" 1) 
COMMENT                                   #102=(CLinePar 
COMMENT                                         (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                         "   /product=\"beta-lactamase\"" 1))) 
COMMENT                                 "AmpR"))) "CDS (3 total)") 
COMMENT                     #103=(CFolderPar 
COMMENT                           (CGroupPar (CParagraph 19 (7 19 0) 1 1 1 0 178) 
COMMENT                            (CObjectList 
COMMENT                             #104=(CFolderPar 
COMMENT                                   (CGroupPar 
COMMENT                                    (CParagraph 228 (3 #7# 0) 1 2 2 0 194) 
COMMENT                                    (CObjectList 
COMMENT                                     #105=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "Start: 228   End: 408 " 1) 
COMMENT                                     #106=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "Original Location Description:" 1) 
COMMENT                                     #107=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "   228..408" 1))) "5' LTR (RU5)") 
COMMENT                             #108=(CFolderPar 
COMMENT                                   (CGroupPar 
COMMENT                                    (CParagraph 7029 (3 #18# 0) 1 2 2 0 194) 
COMMENT                                    (CObjectList 
COMMENT                                     #109=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "Start: 7029  End: 7262" 1) 
COMMENT                                     #110=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "Original Location Description:" 1) 
COMMENT                                     #111=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "   7029..7262" 1))) 
COMMENT                                   "3' LTR (Delta-U3RU5)"))) "LTR (2 total)") 
COMMENT                     #112=(CFolderPar 
COMMENT                           (CGroupPar (CParagraph 21 (7 21 0) 1 2 1 0 178) 
COMMENT                            (CObjectList 
COMMENT                             #113=(CFolderPar 
COMMENT                                   (CGroupPar 
COMMENT                                    (CParagraph 410 (3 #8# 0) 1 2 2 0 194) 
COMMENT                                    (CObjectList 
COMMENT                                     #114=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "Start: 410   End: 563 " 1) 
COMMENT                                     #115=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "Original Location Description:" 1) 
COMMENT                                     #116=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "   410..563" 1))) "SL123") 
COMMENT                             #117=(CFolderPar 
COMMENT                                   (CGroupPar 
COMMENT                                    (CParagraph 455 (3 #9# 0) 1 2 2 0 194) 
COMMENT                                    (CObjectList 
COMMENT                                     #118=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "Start: 455   End: 580 " 1) 
COMMENT                                     #119=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "Original Location Description:" 1) 
COMMENT                                     #120=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "   455..580" 1))) "HIV-1 Psi") 
COMMENT                             #121=(CFolderPar 
COMMENT                                   (CGroupPar 
COMMENT                                    (CParagraph 1073 (3 #11# 0) 1 2 2 0 194) 
COMMENT                                    (CObjectList 
COMMENT                                     #122=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "Start: 1073  End: 1306" 1) 
COMMENT                                     #123=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "Original Location Description:" 1) 
COMMENT                                     #124=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "   1073..1306" 1))) "RRE") 
COMMENT                             #125=(CFolderPar 
COMMENT                                   (CGroupPar 
COMMENT                                    (CParagraph 1802 (3 #12# 0) 1 2 2 0 194) 
COMMENT                                    (CObjectList 
COMMENT                                     #126=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "Start: 1802  End: 1919" 1) 
COMMENT                                     #127=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "Original Location Description:" 1) 
COMMENT                                     #128=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "   1802..1919" 1))) "cPPT/CTS") 
COMMENT                             #129=(CFolderPar 
COMMENT                                   (CGroupPar 
COMMENT                                    (CParagraph 3151 (3 #37# 0) 1 2 2 0 194) 
COMMENT                                    (CObjectList 
COMMENT                                     #130=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "Start: 3151  End: 3216" 1) 
COMMENT                                     #131=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "Original Location Description:" 1) 
COMMENT                                     #132=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "   3151..3216" 1))) 
COMMENT                                   "GMCSF Leader Signal") 
COMMENT                             #133=(CFolderPar 
COMMENT                                   (CGroupPar 
COMMENT                                    (CParagraph 3217 (3 #39# 0) 1 2 2 0 194) 
COMMENT                                    (CObjectList 
COMMENT                                     #134=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "Start: 3217  End: 3564" 1) 
COMMENT                                     #135=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "Original Location Description:" 1) 
COMMENT                                     #136=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "   3217..3564" 1))) "J1.F1 VH") 
COMMENT                             #137=(CFolderPar 
COMMENT                                   (CGroupPar 
COMMENT                                    (CParagraph 3565 (3 #38# 0) 1 2 2 0 194) 
COMMENT                                    (CObjectList 
COMMENT                                     #138=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "Start: 3565  End: 3609" 1) 
COMMENT                                     #139=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "Original Location Description:" 1) 
COMMENT                                     #140=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "   3565..3609" 1))) 
COMMENT                                   "(GGGGS)3 linker") 
COMMENT                             #141=(CFolderPar 
COMMENT                                   (CGroupPar 
COMMENT                                    (CParagraph 3610 (3 #40# 0) 1 2 2 1 194) 
COMMENT                                    (CObjectList 
COMMENT                                     #142=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "Start: 3610  End: 3930" 1) 
COMMENT                                     #143=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "Original Location Description:" 1) 
COMMENT                                     #144=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "   3610..3930" 1))) "J1.F1 VL") 
COMMENT                             #145=(CFolderPar 
COMMENT                                   (CGroupPar 
COMMENT                                    (CParagraph 3931 (3 #32# 0) 1 2 2 0 194) 
COMMENT                                    (CObjectList 
COMMENT                                     #146=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "Start: 3931  End: 3939" 1) 
COMMENT                                     #147=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "Original Location Description:" 1) 
COMMENT                                     #148=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "   3931..3939" 1))) 
COMMENT                                   "AAA linker (also NotI)") 
COMMENT                             #149=(CFolderPar 
COMMENT                                   (CGroupPar 
COMMENT                                    (CParagraph 3940 (3 #33# 0) 1 2 2 0 194) 
COMMENT                                    (CObjectList 
COMMENT                                     #150=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "Start: 3940  End: 4146" 1) 
COMMENT                                     #151=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "Original Location Description:" 1) 
COMMENT                                     #152=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "   3940..4146" 1))) "CD8A") 
COMMENT                             #153=(CFolderPar 
COMMENT                                   (CGroupPar 
COMMENT                                    (CParagraph 4075 (3 #34# 0) 1 2 2 0 194) 
COMMENT                                    (CObjectList 
COMMENT                                     #154=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "Start: 4075  End: 4146" 1) 
COMMENT                                     #155=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "Original Location Description:" 1) 
COMMENT                                     #156=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "   4075..4146" 1))) "TM region") 
COMMENT                             #157=(CFolderPar 
COMMENT                                   (CGroupPar 
COMMENT                                    (CParagraph 4147 (3 #35# 0) 1 2 2 0 194) 
COMMENT                                    (CObjectList 
COMMENT                                     #158=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "Start: 4147  End: 4272" 1) 
COMMENT                                     #159=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "Original Location Description:" 1) 
COMMENT                                     #160=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "   4147..4272" 1))) "41BB") 
COMMENT                             #161=(CFolderPar 
COMMENT                                   (CGroupPar 
COMMENT                                    (CParagraph 4273 (3 #36# 0) 1 2 2 0 194) 
COMMENT                                    (CObjectList 
COMMENT                                     #162=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "Start: 4273  End: 4611" 1) 
COMMENT                                     #163=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "Original Location Description:" 1) 
COMMENT                                     #164=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "   4273..4611" 1))) "CD3Z") 
COMMENT                             #165=(CFolderPar 
COMMENT                                   (CGroupPar 
COMMENT                                    (CParagraph 4618 (3 #14# 0) 1 2 2 0 194) 
COMMENT                                    (CObjectList 
COMMENT                                     #166=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "Start: 4618  End: 4685" 1) 
COMMENT                                     #167=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "Original Location Description:" 1) 
COMMENT                                     #168=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "   4618..4685" 1))) 
COMMENT                                   "Spacer for EMCV IRES") 
COMMENT                             #169=(CFolderPar 
COMMENT                                   (CGroupPar 
COMMENT                                    (CParagraph 4686 (3 #15# 0) 1 2 2 0 194) 
COMMENT                                    (CObjectList 
COMMENT                                     #170=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "Start: 4686  End: 5246" 1) 
COMMENT                                     #171=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "Original Location Description:" 1) 
COMMENT                                     #172=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "   4686..5246" 1))) "EMCV IRES") 
COMMENT                             #173=(CFolderPar 
COMMENT                                   (CGroupPar 
COMMENT                                    (CParagraph 5253 (3 #31# 0) 1 2 2 0 194) 
COMMENT                                    (CObjectList 
COMMENT                                     #174=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "Start: 5253  End: 6347" 1) 
COMMENT                                     #175=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "Original Location Description:" 1) 
COMMENT                                     #176=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "   5253..6347" 1))) "GFP-ZEO") 
COMMENT                             #177=(CFolderPar 
COMMENT                                   (CGroupPar 
COMMENT                                    (CParagraph 6354 (3 #16# 0) 1 2 2 0 194) 
COMMENT                                    (CObjectList 
COMMENT                                     #178=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "Start: 6354  End: 6942" 1) 
COMMENT                                     #179=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "Original Location Description:" 1) 
COMMENT                                     #180=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "   6354..6942" 1))) "WPRE"))) 
COMMENT                           "Misc. Feature (17 total)") 
COMMENT                     #181=(CFolderPar 
COMMENT                           (CGroupPar (CParagraph 25 (7 25 0) 1 1 1 0 178) 
COMMENT                            (CObjectList 
COMMENT                             #182=(CFolderPar 
COMMENT                                   (CGroupPar 
COMMENT                                    (CParagraph 7334 (3 #19# 0) 1 2 2 0 194) 
COMMENT                                    (CObjectList 
COMMENT                                     #183=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "Start: 7334  End: 7455" 1) 
COMMENT                                     #184=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "Original Location Description:" 1) 
COMMENT                                     #185=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "   7334..7455" 1))) 
COMMENT                                   "SV40 poly(A) signal"))) 
COMMENT                           "PolyA Signal (1 total)") 
COMMENT                     #186=(CFolderPar 
COMMENT                           (CGroupPar (CParagraph 30 (7 30 0) 1 1 1 0 178) 
COMMENT                            (CObjectList 
COMMENT                             #187=(CFolderPar 
COMMENT                                   (CGroupPar 
COMMENT                                    (CParagraph 1 (3 #6# 0) 1 2 2 0 194) 
COMMENT                                    (CObjectList 
COMMENT                                     #188=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "Start: 1     End: 227 " 1) 
COMMENT                                     #189=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "Original Location Description:" 1) 
COMMENT                                     #190=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "   1..227" 1))) "RSV promoter") 
COMMENT                             #191=(CFolderPar 
COMMENT                                   (CGroupPar 
COMMENT                                    (CParagraph 1944 (3 #13# 0) 1 2 2 0 194) 
COMMENT                                    (CObjectList 
COMMENT                                     #192=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "Start: 1944  End: 3122" 1) 
COMMENT                                     #193=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "Original Location Description:" 1) 
COMMENT                                     #194=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "   1944..3122" 1))) "EF-1a") 
COMMENT                             #195=(CFolderPar 
COMMENT                                   (CGroupPar 
COMMENT                                    (CParagraph 7651 (3 #21# 0) 1 2 2 0 194) 
COMMENT                                    (CObjectList 
COMMENT                                     #196=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "Start: 7651  End: 7669 (Complementary)" 
COMMENT                                           1) 
COMMENT                                     #197=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "Original Location Description:" 1) 
COMMENT                                     #198=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "   complement(7651..7669)" 1))) 
COMMENT                                   "T7 promoter") 
COMMENT                             #199=(CFolderPar 
COMMENT                                   (CGroupPar 
COMMENT                                    (CParagraph 8318 (3 #23# 0) 1 2 2 0 194) 
COMMENT                                    (CObjectList 
COMMENT                                     #200=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "Start: 8318  End: 8422" 1) 
COMMENT                                     #201=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "Original Location Description:" 1) 
COMMENT                                     #202=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "   8318..8422" 1) 
COMMENT                                     #203=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "Qualifiers:" 1) 
COMMENT                                     #204=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "   /gene=\"bla\"" 1))) 
COMMENT                                   "AmpR promoter") 
COMMENT                             #205=(CFolderPar 
COMMENT                                   (CGroupPar 
COMMENT                                    (CParagraph 10366 (3 #27# 0) 1 2 2 0 194) 
COMMENT                                    (CObjectList 
COMMENT                                     #206=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "Start: 10366  End: 10396" 1) 
COMMENT                                     #207=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "Original Location Description:" 1) 
COMMENT                                     #208=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "   10366..10396" 1))) "lac promoter") 
COMMENT                             #209=(CFolderPar 
COMMENT                                   (CGroupPar 
COMMENT                                    (CParagraph 10465 (3 #29# 0) 1 2 2 0 194) 
COMMENT                                    (CObjectList 
COMMENT                                     #210=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "Start: 10465  End: 1   " 1) 
COMMENT                                     #211=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "Original Location Description:" 1) 
COMMENT                                     #212=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "   10465..1" 1))) "Promoter_P_1") 
COMMENT                             #213=(CFolderPar 
COMMENT                                   (CGroupPar 
COMMENT                                    (CParagraph 10465 (3 #30# 0) 1 2 2 0 194) 
COMMENT                                    (CObjectList 
COMMENT                                     #214=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "Start: 10465  End: 10483" 1) 
COMMENT                                     #215=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "Original Location Description:" 1) 
COMMENT                                     #216=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "   10465..10483" 1))) "T3 promoter"))) 
COMMENT                           "Promoter Prokaryotic (7 total)") 
COMMENT                     #217=(CFolderPar 
COMMENT                           (CGroupPar (CParagraph 31 (7 31 0) 1 1 1 0 178) 
COMMENT                            (CObjectList 
COMMENT                             #218=(CFolderPar 
COMMENT                                   (CGroupPar 
COMMENT                                    (CParagraph 10330 (3 #26# 0) 1 2 2 0 194) 
COMMENT                                    (CObjectList 
COMMENT                                     #219=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "Start: 10330  End: 10351" 1) 
COMMENT                                     #220=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "Original Location Description:" 1) 
COMMENT                                     #221=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "   10330..10351" 1) 
COMMENT                                     #222=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "Qualifiers:" 1) 
COMMENT                                     #223=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "   /bound_moiety=\"E. coli catabolite activator protein\"" 
COMMENT                                           1))) "CAP binding site") 
COMMENT                             #224=(CFolderPar 
COMMENT                                   (CGroupPar 
COMMENT                                    (CParagraph 10404 (3 #28# 0) 1 2 2 0 194) 
COMMENT                                    (CObjectList 
COMMENT                                     #225=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "Start: 10404  End: 10420" 1) 
COMMENT                                     #226=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "Original Location Description:" 1) 
COMMENT                                     #227=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "   10404..10420" 1) 
COMMENT                                     #228=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "Qualifiers:" 1) 
COMMENT                                     #229=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "   /bound_moiety=\"lac repressor encoded by lacI\"" 
COMMENT                                           1))) "lac operator"))) 
COMMENT                           "Protein Binding Site (2 total)") 
COMMENT                     #230=(CFolderPar 
COMMENT                           (CGroupPar (CParagraph 33 (7 33 0) 1 1 1 0 178) 
COMMENT                            (CObjectList 
COMMENT                             #231=(CFolderPar 
COMMENT                                   (CGroupPar 
COMMENT                                    (CParagraph 7495 (3 #20# 0) 1 2 2 0 194) 
COMMENT                                    (CObjectList 
COMMENT                                     #232=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "Start: 7495  End: 7630" 1) 
COMMENT                                     #233=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "Original Location Description:" 1) 
COMMENT                                     #234=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "   7495..7630" 1))) "SV40 ori") 
COMMENT                             #235=(CFolderPar 
COMMENT                                   (CGroupPar 
COMMENT                                    (CParagraph 7837 (3 #22# 0) 1 2 2 0 194) 
COMMENT                                    (CObjectList 
COMMENT                                     #236=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "Start: 7837  End: 8292" 1) 
COMMENT                                     #237=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "Original Location Description:" 1) 
COMMENT                                     #238=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "   7837..8292" 1) 
COMMENT                                     #239=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "Qualifiers:" 1) 
COMMENT                                     #240=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "   /direction=RIGHT" 1))) "f1 ori") 
COMMENT                             #241=(CFolderPar 
COMMENT                                   (CGroupPar 
COMMENT                                    (CParagraph 9454 (3 #25# 0) 1 2 2 0 194) 
COMMENT                                    (CObjectList 
COMMENT                                     #242=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "Start: 9454  End: 10042" 1) 
COMMENT                                     #243=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "Original Location Description:" 1) 
COMMENT                                     #244=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "   9454..10042" 1) 
COMMENT                                     #245=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "Qualifiers:" 1) 
COMMENT                                     #246=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "   /direction=RIGHT" 1))) "ori"))) 
COMMENT                           "Replication Origin (3 total)"))) "Feature Map") 
COMMENT              #247=(CFolderPar 
COMMENT                    (CGroupPar (CParagraph 12 (6 0) 1 1 0 0 178) 
COMMENT                     (CObjectList 
COMMENT                      #248=(CFolderPar 
COMMENT                            (CGroupPar (CParagraph 98 (0 0) 1 1 1 0 178) 
COMMENT                             (CObjectList 
COMMENT                              #249=(CFolderPar 
COMMENT                                    (CGroupPar (CParagraph 1 (0 0) 1 2 2 0 178) 
COMMENT                                     (CObjectList 
COMMENT                                      #250=(CLinePar 
COMMENT                                            (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                            "Start: 1     End: 10510" 1) 
COMMENT                                      #251=(CLinePar 
COMMENT                                            (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                            "Original Location Description:" 1) 
COMMENT                                      #252=(CLinePar 
COMMENT                                            (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                            "   1..10510" 1) 
COMMENT                                      #253=(CLinePar 
COMMENT                                            (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                            "Qualifiers:" 1) 
COMMENT                                      #254=(CLinePar 
COMMENT                                            (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                            "   /organism=\"synthetic DNA construct\"" 
COMMENT                                            1) 
COMMENT                                      #255=(CLinePar 
COMMENT                                            (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                            "   /mol_type=\"other DNA\"" 1))) 
COMMENT                                    "Source_1"))) "Source (1 total)"))) 
COMMENT                    "Imported Features Not Shown on Map") 
COMMENT              #256=(CFolderPar 
COMMENT                    (CGroupPar (CParagraph 13 (0 0) 1 1 0 0 178) 
COMMENT                     (CObjectList 
COMMENT                      #257=(CRSFolderPar 
COMMENT                            (CFolderPar 
COMMENT                             (CGroupPar (CParagraph 63911280 (8 0) 1 1 1 0 178) 
COMMENT                              (CObjectList 
COMMENT                               #258=(CGroupPar 
COMMENT                                     (CParagraph 0 (10 #1# 0) 1 2 2 0 180) 
COMMENT                                     (CObjectList 
COMMENT                                      #259=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #1# 1) 1 2 2 0 191) 
COMMENT                                            " N1: 4613 " 1))))) "BsiWI: 1 site") 
COMMENT                            1 5 "CGTACG" "GCATGC") 
COMMENT                      #260=(CRSFolderPar 
COMMENT                            (CFolderPar 
COMMENT                             (CGroupPar (CParagraph 63911216 (8 0) 1 1 1 0 178) 
COMMENT                              (CObjectList 
COMMENT                               #261=(CGroupPar 
COMMENT                                     (CParagraph 0 (10 #0# 0) 1 2 2 0 180) 
COMMENT                                     (CObjectList 
COMMENT                                      #262=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #0# 1) 1 2 2 0 191) 
COMMENT                                            " N1: 3140 " 1))))) "EcoRI: 1 site") 
COMMENT                            1 5 "GAATTC" "CTTAAG") 
COMMENT                      #263=(CRSFolderPar 
COMMENT                            (CFolderPar 
COMMENT                             (CGroupPar (CParagraph 100923392 (8 0) 1 1 1 0 178) 
COMMENT                              (CObjectList)) "NheI: 0 sites") 1 5 "GCTAGC" 
COMMENT                            "CGATCG") 
COMMENT                      #264=(CRSFolderPar 
COMMENT                            (CFolderPar 
COMMENT                             (CGroupPar (CParagraph 100923456 (8 0) 1 1 1 0 178) 
COMMENT                              (CObjectList 
COMMENT                               #265=(CGroupPar 
COMMENT                                     (CParagraph 0 (10 #2# 0) 1 2 2 0 180) 
COMMENT                                     (CObjectList 
COMMENT                                      #266=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #2# 1) 1 2 2 0 191) 
COMMENT                                            " N1: 3933 " 1))))) "NotI: 1 site") 
COMMENT                            2 6 "GCGGCCGC" "CGCCGGCG") 
COMMENT                      #267=(CRSFolderPar 
COMMENT                            (CFolderPar 
COMMENT                             (CGroupPar (CParagraph 100923136 (8 0) 1 1 1 0 178) 
COMMENT                              (CObjectList 
COMMENT                               #268=(CGroupPar 
COMMENT                                     (CParagraph 0 (10 #4# 0) 1 2 2 0 180) 
COMMENT                                     (CObjectList 
COMMENT                                      #269=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #4# 1) 1 2 2 0 191) 
COMMENT                                            " N1: 6349 " 1))))) "SalI: 1 site") 
COMMENT                            1 5 "GTCGAC" "CAGCTG") 
COMMENT                      #270=(CRSFolderPar 
COMMENT                            (CFolderPar 
COMMENT                             (CGroupPar (CParagraph 100917728 (8 0) 1 1 1 0 178) 
COMMENT                              (CObjectList 
COMMENT                               #271=(CGroupPar 
COMMENT                                     (CParagraph 0 (10 #5# 0) 1 2 2 0 180) 
COMMENT                                     (CObjectList 
COMMENT                                      #272=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #5# 1) 1 2 2 0 191) 
COMMENT                                            " N1: 5248 " 1))))) "SpeI: 1 site") 
COMMENT                            1 5 "ACTAGT" "TGATCA"))) 
COMMENT                    "Restriction/Methylation Map")))) 
COMMENT       (CGraphView 
COMMENT        (CWStyleSheet 
COMMENT         (CObjectList 
COMMENT          #273=(CWidgetStyle "RSite Label" 1 (LOGPEN 0 0 13408563) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 18 "Georgia") 0.555556 0 1 5 
COMMENT                "@N (@S)" 0) 
COMMENT          #274=(CWidgetStyle "Signal Label" 1 (LOGPEN 0 0 0) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 700 0 0 0 0 3 2 1 34 "Arial") 0.666667 0 1 1 
COMMENT                "@N" 0) 
COMMENT          #275=(CWidgetStyle "Molecule Label 2" 0 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 18 "Georgia") 0.555556 0 1 16 
COMMENT                "@L bp" 0) 
COMMENT          #276=(CWidgetStyle "Molecule Label 1" 0 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 34 "Verdana") 0.833333 0 1 1 
COMMENT                "@N" 0) 
COMMENT          #277=(CWidgetStyle "Shape 3" 1 (LOGPEN 0 0 3355545) 1 1 
COMMENT                (LOGBRUSH 0 6724095 0) 0 0 1 (LOGSHAPE 9 1 0.8 1.8 0)) 
COMMENT          #278=(CWidgetStyle "Shape 1" 1 (LOGPEN 0 0 6723840) 1 1 
COMMENT                (LOGBRUSH 0 10079334 0) 0 0 1 (LOGSHAPE 9 1 0.8 1.8 0)) 
COMMENT          #279=(CWidgetStyle "Axis" 1 (LOGPEN 0 0 10079436) 2 1 
COMMENT                (LOGBRUSH 0 13434879 0) 0 0 1 (LOGSHAPE 10 1 0 0 0)) 
COMMENT          #280=(CWidgetStyle "Line 2" 1 (LOGPEN 0 0 6723840) 8 0 0 0 1 
COMMENT                (LOGSHAPE 1 1.9 0 0 0)) 
COMMENT          #281=(CWidgetStyle "RSite" 1 (LOGPEN 0 0 10053171) 8 0 0 0 1 
COMMENT                (LOGSHAPE 1 1.9 0 0 0)) 
COMMENT          #282=(CWidgetStyle "Short Signal" 1 (LOGPEN 0 0 13395507) 10 0 0 0 1 
COMMENT                (LOGSHAPE 1 1.9 0 0 0)) 
COMMENT          #283=(CWidgetStyle "Uniq RSite Label" 1 (LOGPEN 0 0 153) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 18 "Georgia") 0.555556 128 1 
COMMENT                5 "@N (@S)" 0) 
COMMENT          #284=(CWidgetStyle "Vanilla" 1 (LOGPEN 0 0 0) 1 1 
COMMENT                (LOGBRUSH 0 16777215 0) 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 7 48 2 18 "Times New Roman") 0.8 0 
COMMENT                1 2 "?" 0) 
COMMENT          #285=(CWidgetStyle "Mark 1" 0 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 2 7 48 2 2 "Windings") 0.7 0 1 2 "?" 
COMMENT                0) 
COMMENT          #286=(CWidgetStyle "Motif Label" 1 (LOGPEN 0 0 16744512) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 34 "Arial") 0.611111 8388608 
COMMENT                1 65535 "@N (@H)" 0) 
COMMENT          #287=(CWidgetStyle "Fragment Label 2" 1 (LOGPEN 0 0 0) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 49 "Courier New") 1.05 0 1 48 
COMMENT                "@F bp (molecule @L bp)" 0) 
COMMENT          #288=(CWidgetStyle "Fragment Label 1" 1 (LOGPEN 0 0 0) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 34 "Arial") 0.91 0 1 1 
COMMENT                "Fragment of @N" 0) 
COMMENT          #289=(CWidgetStyle "Shape 4" 1 (LOGPEN 0 0 0) 1 1 
COMMENT                (LOGBRUSH 2 8388608 5) 0 0 0) 
COMMENT          #290=(CWidgetStyle "Shape 2" 1 (LOGPEN 0 0 0) 1 1 (LOGBRUSH 0 128 0) 0 
COMMENT                0 0) 
COMMENT          #291=(CWidgetStyle "Shape 0" 1 (LOGPEN 0 0 0) 1 1 (LOGBRUSH 0 0 0) 0 0 
COMMENT                0) 
COMMENT          #292=(CWidgetStyle "ORF" 1 (LOGPEN 0 0 16384) 8 0 0 0 1 
COMMENT                (LOGSHAPE 7 0.2 3.41182 2.86186 0.609808)) 
COMMENT          #293=(CWidgetStyle "Line 4" 1 (LOGPEN 0 0 32768) 8 0 0 0 0) 
COMMENT          #294=(CWidgetStyle "Line 3" 1 (LOGPEN 0 0 16711680) 8 0 0 0 0) 
COMMENT          #295=(CWidgetStyle "Line 1" 1 (LOGPEN 0 0 16711680) 1 0 0 0 0) 
COMMENT          #296=(CWidgetStyle "Short Promoter" 1 (LOGPEN 0 0 128) 6 0 0 0 0) 
COMMENT          #297=(CWidgetStyle "Motif" 1 (LOGPEN 0 0 0) 1 0 0 0 0) 
COMMENT          #298=(CWidgetStyle "Line 0" 1 (LOGPEN 0 0 0) 8 0 0 0 0) 
COMMENT          #299=(CWidgetStyle "Void" 0 0 0 0 0) 
COMMENT          #300=(CWidgetStyle "General Label" 1 (LOGPEN 0 0 0) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 18 "Times New Roman") 0.91 0 
COMMENT                1 3 "@T @N " 0) 
COMMENT          #301=(CWidgetStyle "Position" 1 (LOGPEN 0 0 0) 1 0 0 0 0) 
COMMENT          #302=(CWidgetStyle "Annotation" 0 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 18 "Times New Roman") 0.91 0 
COMMENT                0 0) 
COMMENT          #303=(CWidgetStyle "Position Label" 1 (LOGPEN 0 0 8388608) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 1 0 0 3 2 1 34 "Arial") 0.63 8388608 1 1 
COMMENT                "@N" 0) 
COMMENT          #304=(CWidgetStyle "Range" 1 (LOGPEN 0 0 0) 1 1 
COMMENT                (LOGBRUSH 0 16777215 0) 0 0 0) 
COMMENT          #305=(CWidgetStyle "Range Label" 1 (LOGPEN 0 0 8388608) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 1 0 0 3 2 1 34 "Arial") 0.63 8388608 1 1 
COMMENT                "@N" 0) 
COMMENT          #306=(CWidgetStyle "ORF Label" 1 (LOGPEN 0 0 49216) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 18 "Times New Roman") 
COMMENT                0.611111 0 1 65535 "@N" 0) 
COMMENT          #307=(CWidgetStyle "CDS Label" 1 (LOGPEN 0 0 4227264) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 34 "Arial") 0.555556 255 1 1 
COMMENT                "@N" 0) 
COMMENT          #308=(CWidgetStyle "Shape 5" 1 (LOGPEN 0 0 0) 3 1 
COMMENT                (LOGBRUSH 0 16777113 0) 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 7 48 2 50 "Arial") 0.9 0 0 1 
COMMENT                (LOGSHAPE 9 1 0.8 1.8 0)) 
COMMENT          #309=(CWidgetStyle "CDS" 1 (LOGPEN 0 0 0) 1 1 (LOGBRUSH 2 39423 3) 0 0 
COMMENT                1 (LOGSHAPE 9 1 0.8 1.8 0)) 
COMMENT          #310=(CWidgetStyle "Label 2" 1 (LOGPEN 0 0 4227264) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 34 "Arial") 0.944444 8388608 
COMMENT                1 1 "@N" 0) 
COMMENT          #311=(CWidgetStyle "Label 3" 1 (LOGPEN 0 0 8421376) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 700 255 0 0 0 3 2 1 34 "Arial") 0.833333 255 1 
COMMENT                5 "@N (@S)" 0) 
COMMENT          #312=(CWidgetStyle "Label 4" 1 (LOGPEN 0 0 8437824) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 34 "Arial") 0.722222 0 1 5 
COMMENT                "@N (@S)" 0)) 0.164644 1.74233 0.164644 2.53336 
COMMENT         (70 (CShapeMapEntry 0 "Unique RSite" 1 "Uniq RSite Label") 67 
COMMENT          (CShapeMapEntry 0 "ORF" 0 "ORF Label")) 40.0378 40.0378 39 39 0.1 -365) 
COMMENT        1 1 0 1 1 
COMMENT        (mapper: 26.3213 -23.1844 40.0378 40.0378 0.01 10 -1 10510 10510 1 0 0) 
COMMENT        #313=(CGroupWidget (CWidget 0 (0 0) 1 2 0 0 Nil 695412850 100) 
COMMENT              (CObjectList 
COMMENT               #314=(CGroupWidget (CWidget 1 (0 0) 1 2 0 0 Nil -1641 100) 
COMMENT                     (CObjectList 
COMMENT                      #315=(CAxis 
COMMENT                            (CWideLine 
COMMENT                             (CWidget 0 (0 0) 1 2 0 0 #279# 100144556 0) 
COMMENT                             (LOGPEN 0 0 10079436) 2 (LOGBRUSH 0 13434879 0) 1 
COMMENT                             6.27478 6.27278 1 0.0214037) 0.0596878) 
COMMENT                      #316=(CLabel 
COMMENT                            (CWidget 1001 (0 0) 1 2 0 0 #276# -576706490 100) 
COMMENT                            (LOGPEN 0 0 0) 1 
COMMENT                            (LOGFONT 58 22 0 0 400 0 0 0 0 3 2 1 34 "Verdana") 
COMMENT                            1.74233 0.833333 0 "pRRL.SIN.EF1A.JAG1-F1.GS.CAR-2G" 
COMMENT                            "@N" 1 0 0.871165 0 -10 20.8053 1.39868 Nil) 
COMMENT                      #317=(CLabel (CWidget 1002 (0 0) 1 2 0 0 #275# 0 100) 
COMMENT                            (LOGPEN 0 0 0) 1 
COMMENT                            (LOGFONT 38 14 0 0 400 0 0 0 0 3 2 1 18 "Georgia") 
COMMENT                            1.74233 0.555556 0 "10510 bp" "@L bp" 16 0 -0.871165 
COMMENT                            0 -10.8 2.77238 0.799245 Nil)) (CObjectList)) 
COMMENT               #318=(CGroupWidget (CWidget 10 (6 0) 1 2 0 0 Nil -1502 100) 
COMMENT                     (CObjectList 
COMMENT                      #319=(CGroupWidget 
COMMENT                            (CWidget 4 (7 4 0) 1 2 0 0 Nil -391 100) 
COMMENT                            (CObjectList 
COMMENT                             #320=(CWideArrow 
COMMENT                                   (CWideLine 
COMMENT                                    (CWidget 0 (3 #10# 0) 1 2 0 0 #277# 0 100) 
COMMENT                                    (LOGPEN 0 0 3355545) 1 
COMMENT                                    (LOGBRUSH 0 6724095 0) 1 5.71988 5.93714 1 
COMMENT                                    0.082322) 0.8 1.8 0) 
COMMENT                             #321=(CLabel 
COMMENT                                   (CWidget 0 (0 0) 1 2 0 0 #274# 268730864 100) 
COMMENT                                   (LOGPEN 0 0 0) 1 
COMMENT                                   (LOGFONT 46 17 0 0 700 0 0 0 0 3 2 1 34 
COMMENT                                    "Arial") 1.74233 0.666667 0 "SL4 (Gag)" "@N" 
COMMENT                                   1 15.618 10.454 0 -10 4.32092 1.04901 #320#) 
COMMENT                             #322=(CScratch 
COMMENT                                   (CWidget 0 (3 #17# 0) 1 2 0 0 #282# 845 100) 
COMMENT                                   (LOGPEN 0 8 13395507) 10 1 2.20009 1.9 
COMMENT                                   0.082322 1) 
COMMENT                             #323=(CLabel 
COMMENT                                   (CWidget 0 (0 0) 1 2 0 0 #274# 1735681891 100) 
COMMENT                                   (LOGPEN 0 0 0) 1 
COMMENT                                   (LOGFONT 46 17 0 0 700 0 0 0 0 3 2 1 34 
COMMENT                                    "Arial") 1.74233 0.666667 0 "Factor Xa site" 
COMMENT                                   "@N" 1 -16.8995 -10.454 0 -10 6.09424 1.04901 
COMMENT                                   #322#) 
COMMENT                             #324=(CWideArrow 
COMMENT                                   (CWideLine 
COMMENT                                    (CWidget 0 (3 #24# 0) 1 2 0 0 #277# 0 100) 
COMMENT                                    (LOGPEN 0 0 3355545) 1 
COMMENT                                    (LOGBRUSH 0 6724095 0) 1 0.732369 1.24628 1 
COMMENT                                    0.082322) 0.8 1.8 0) 
COMMENT                             #325=(CLabel (CWidget 0 (0 0) 1 2 0 0 #274# 0 100) 
COMMENT                                   (LOGPEN 0 0 0) 1 
COMMENT                                   (LOGFONT 46 17 0 0 700 0 0 0 0 3 2 1 34 
COMMENT                                    "Arial") 1.74233 0.666667 0 "AmpR" "@N" 1 
COMMENT                                   -15.9327 8.71165 0 -10 2.6475 1.04901 #324#)) 
COMMENT                            (CObjectList)) 
COMMENT                      #326=(CGroupWidget 
COMMENT                            (CWidget 19 (7 19 0) 1 2 0 0 Nil -1920 100) 
COMMENT                            (CObjectList 
COMMENT                             #327=(CWideArrow 
COMMENT                                   (CWideLine 
COMMENT                                    (CWidget 0 (3 #7# 0) 1 2 0 0 #289# 0 100) 
COMMENT                                    (LOGPEN 0 0 0) 1 (LOGBRUSH 2 8388608 5) 1 
COMMENT                                    6.02966 6.13769 1 0.082322) 0.8 1.8 0) 
COMMENT                             #328=(CLabel 
COMMENT                                   (CWidget 0 (0 0) 1 2 0 0 #274# 268730828 100) 
COMMENT                                   (LOGPEN 0 0 0) 1 
COMMENT                                   (LOGFONT 46 17 0 0 700 0 0 0 0 3 2 1 34 
COMMENT                                    "Arial") 1.74233 0.666667 0 "5' LTR (RU5)" 
COMMENT                                   "@N" 1 9.25547 15.681 0 -10 5.22007 1.04901 
COMMENT                                   #327#) 
COMMENT                             #329=(CWideArrow 
COMMENT                                   (CWideLine 
COMMENT                                    (CWidget 0 (3 #18# 0) 1 2 0 0 #289# 0 100) 
COMMENT                                    (LOGPEN 0 0 0) 1 (LOGBRUSH 2 8388608 5) 1 
COMMENT                                    1.93866 2.07833 1 0.082322) 0.8 1.8 0) 
COMMENT                             #330=(CLabel 
COMMENT                                   (CWidget 0 (0 0) 1 2 0 0 #274# 268730864 100) 
COMMENT                                   (LOGPEN 0 0 0) 1 
COMMENT                                   (LOGFONT 46 17 0 0 700 0 0 0 0 3 2 1 34 
COMMENT                                    "Arial") 1.74233 0.666667 0 
COMMENT                                   "3' LTR (Delta-U3RU5)" "@N" 1 -19.8654 
COMMENT                                   -6.96932 0 -10 8.81667 1.04901 #329#)) 
COMMENT                            (CObjectList)) 
COMMENT                      #331=(CGroupWidget 
COMMENT                            (CWidget 21 (7 21 0) 1 2 0 0 Nil -1153 100) 
COMMENT                            (CObjectList 
COMMENT                             #332=(CWideArrow 
COMMENT                                   (CWideLine 
COMMENT                                    (CWidget 0 (3 #8# 0) 1 2 0 0 #278# 0 100) 
COMMENT                                    (LOGPEN 0 0 6723840) 1 
COMMENT                                    (LOGBRUSH 0 10079334 0) 1 5.93714 6.02906 1 
COMMENT                                    0.082322) 0.8 1.8 0) 
COMMENT                             #333=(CLabel 
COMMENT                                   (CWidget 0 (0 0) 1 2 0 0 #274# 268730864 100) 
COMMENT                                   (LOGPEN 0 0 0) 1 
COMMENT                                   (LOGFONT 46 17 0 0 700 0 0 0 0 3 2 1 34 
COMMENT                                    "Arial") 1.74233 0.666667 0 "SL123" "@N" 1 
COMMENT                                   11.2304 13.9386 0 -10 2.82233 1.04901 #332#) 
COMMENT                             #334=(CWideArrow 
COMMENT                                   (CWideLine 
COMMENT                                    (CWidget 0 (3 #9# 0) 1 2 0 0 #278# 0 100) 
COMMENT                                    (LOGPEN 0 0 6723840) 1 
COMMENT                                    (LOGBRUSH 0 10079334 0) 0.835356 5.927 
COMMENT                                    6.0022 1 0.082322) 0.8 1.8 0) 
COMMENT                             #335=(CLabel 
COMMENT                                   (CWidget 0 (0 0) 1 2 0 0 #274# -594269838 100) 
COMMENT                                   (LOGPEN 0 0 0) 1 
COMMENT                                   (LOGFONT 46 17 0 0 700 0 0 0 0 3 2 1 34 
COMMENT                                    "Arial") 1.74233 0.666667 0 "HIV-1 Psi" "@N" 
COMMENT                                   1 13.9006 12.1963 0 -10 4.07115 1.04901 #334#) 
COMMENT                             #336=(CWideArrow 
COMMENT                                   (CWideLine 
COMMENT                                    (CWidget 0 (3 #11# 0) 1 2 0 0 #278# 0 100) 
COMMENT                                    (LOGPEN 0 0 6723840) 1 
COMMENT                                    (LOGBRUSH 0 10079334 0) 1 5.49366 5.63333 1 
COMMENT                                    0.082322) 0.8 1.8 0) 
COMMENT                             #337=(CLabel (CWidget 0 (0 0) 1 2 0 0 #274# 0 100) 
COMMENT                                   (LOGPEN 0 0 0) 1 
COMMENT                                   (LOGFONT 46 17 0 0 700 0 0 0 0 3 2 1 34 
COMMENT                                    "Arial") 1.74233 0.666667 0 "RRE" "@N" 1 
COMMENT                                   15.532 8.71165 0 -10 1.94816 1.04901 #336#) 
COMMENT                             #338=(CWideArrow 
COMMENT                                   (CWideLine 
COMMENT                                    (CWidget 0 (3 #12# 0) 1 2 0 0 #278# 0 100) 
COMMENT                                    (LOGPEN 0 0 6723840) 1 
COMMENT                                    (LOGBRUSH 0 10079334 0) 1 5.12778 5.19821 1 
COMMENT                                    0.082322) 0.8 1.8 0) 
COMMENT                             #339=(CLabel (CWidget 0 (0 0) 1 2 0 0 #274# 10 100) 
COMMENT                                   (LOGPEN 0 0 0) 1 
COMMENT                                   (LOGFONT 46 17 0 0 700 0 0 0 0 3 2 1 34 
COMMENT                                    "Arial") 1.74233 0.666667 0 "cPPT/CTS" "@N" 
COMMENT                                   1 17.5307 6.96932 0 -10 4.24599 1.04901 #338#) 
COMMENT                             #340=(CScratch 
COMMENT                                   (CWidget 0 (3 #14# 0) 1 2 0 0 #282# 845 100) 
COMMENT                                   (LOGPEN 0 8 13395507) 10 1 3.5174 1.9 
COMMENT                                   0.082322 1) 
COMMENT                             #341=(CLabel 
COMMENT                                   (CWidget 0 (0 0) 1 2 0 0 #274# 108727528 100) 
COMMENT                                   (LOGPEN 0 0 0) 1 
COMMENT                                   (LOGFONT 46 17 0 0 700 0 0 0 0 3 2 1 34 
COMMENT                                    "Arial") 1.74233 0.666667 0 
COMMENT                                   "Spacer for EMCV IRES" "@N" 1 11.9643 
COMMENT                                   -26.1349 0 -10 9.89065 1.04901 #340#) 
COMMENT                             #342=(CWideArrow 
COMMENT                                   (CWideLine 
COMMENT                                    (CWidget 0 (3 #15# 0) 1 2 0 0 #278# 0 100) 
COMMENT                                    (LOGPEN 0 0 6723840) 1 
COMMENT                                    (LOGBRUSH 0 10079334 0) 0.835356 3.14196 
COMMENT                                    3.47681 1 0.082322) 0.8 1.8 0) 
COMMENT                             #343=(CLabel 
COMMENT                                   (CWidget 0 (0 0) 1 2 0 0 #274# 538976288 100) 
COMMENT                                   (LOGPEN 0 0 0) 1 
COMMENT                                   (LOGFONT 46 17 0 0 700 0 0 0 0 3 2 1 34 
COMMENT                                    "Arial") 1.74233 0.666667 0 "EMCV IRES" "@N" 
COMMENT                                   1 6.30926 -27.8773 0 -10 5.12016 1.04901 
COMMENT                                   #342#) 
COMMENT                             #344=(CWideArrow 
COMMENT                                   (CWideLine 
COMMENT                                    (CWidget 0 (3 #16# 0) 1 2 0 0 #278# 0 100) 
COMMENT                                    (LOGPEN 0 0 6723840) 1 
COMMENT                                    (LOGBRUSH 0 10079334 0) 0.835356 2.12966 
COMMENT                                    2.48122 1 0.082322) 0.8 1.8 0) 
COMMENT                             #345=(CLabel 
COMMENT                                   (CWidget 0 (0 0) 1 2 0 0 #274# 268730864 100) 
COMMENT                                   (LOGPEN 0 0 0) 1 
COMMENT                                   (LOGFONT 46 17 0 0 700 0 0 0 0 3 2 1 34 
COMMENT                                    "Arial") 1.74233 0.666667 0 "WPRE" "@N" 1 
COMMENT                                   -14.2392 -12.1963 0 -10 2.7474 1.04901 #344#) 
COMMENT                             #346=(CWideArrow 
COMMENT                                   (CWideLine 
COMMENT                                    (CWidget 0 (3 #31# 0) 1 2 0 0 #278# 0 100) 
COMMENT                                    (LOGPEN 0 0 6723840) 1 
COMMENT                                    (LOGBRUSH 0 10079334 0) 1 2.4848 3.13838 1 
COMMENT                                    0.082322) 0.8 1.8 0) 
COMMENT                             #347=(CLabel 
COMMENT                                   (CWidget 0 (0 0) 1 2 0 0 #274# 108299640 100) 
COMMENT                                   (LOGPEN 0 0 0) 1 
COMMENT                                   (LOGFONT 46 17 0 0 700 0 0 0 0 3 2 1 34 
COMMENT                                    "Arial") 1.74233 0.666667 0 "GFP-ZEO" "@N" 1 
COMMENT                                   -8.57596 -15.681 0 -10 4.07115 1.04901 #346#) 
COMMENT                             #348=(CScratch 
COMMENT                                   (CWidget 0 (3 #32# 0) 1 2 0 0 #282# -5645322 
COMMENT                                    100) (LOGPEN 0 8 13395507) 10 1 3.92746 1.9 
COMMENT                                   0.082322 1) 
COMMENT                             #349=(CLabel 
COMMENT                                   (CWidget 0 (0 0) 1 2 0 0 #274# 268730864 100) 
COMMENT                                   (LOGPEN 0 0 0) 1 
COMMENT                                   (LOGFONT 46 17 0 0 700 0 0 0 0 3 2 1 34 
COMMENT                                    "Arial") 1.74233 0.666667 0 
COMMENT                                   "AAA linker (also NotI)" "@N" 1 17.0492 
COMMENT                                   -13.9386 0 -10 9.26624 1.04901 #348#) 
COMMENT                             #350=(CWideArrow 
COMMENT                                   (CWideLine 
COMMENT                                    (CWidget 0 (3 #33# 0) 1 2 0 0 #278# 708 100) 
COMMENT                                    (LOGPEN 0 0 6723840) 1 
COMMENT                                    (LOGBRUSH 0 10079334 0) 0.835356 3.79853 
COMMENT                                    3.92208 1 0.082322) 0.8 1.8 0) 
COMMENT                             #351=(CLabel 
COMMENT                                   (CWidget 0 (0 0) 1 2 0 0 #274# 268730864 100) 
COMMENT                                   (LOGPEN 0 0 0) 1 
COMMENT                                   (LOGFONT 46 17 0 0 700 0 0 0 0 3 2 1 34 
COMMENT                                    "Arial") 1.74233 0.666667 0 "CD8A" "@N" 1 
COMMENT                                   13.0417 -17.4233 0 -10 2.47266 1.04901 #350#) 
COMMENT                             #352=(CScratch 
COMMENT                                   (CWidget 0 (3 #34# 0) 1 2 0 0 #282# -3216646 
COMMENT                                    100) (LOGPEN 0 8 13395507) 10 1 3.84151 1.9 
COMMENT                                   0.082322 1) 
COMMENT                             #353=(CLabel 
COMMENT                                   (CWidget 0 (0 0) 1 2 0 0 #274# 268730864 100) 
COMMENT                                   (LOGPEN 0 0 0) 1 
COMMENT                                   (LOGFONT 46 17 0 0 700 0 0 0 0 3 2 1 34 
COMMENT                                    "Arial") 1.74233 0.666667 0 "TM region" "@N" 
COMMENT                                   1 13.8698 -19.1656 0 -10 4.32092 1.04901 
COMMENT                                   #352#) 
COMMENT                             #354=(CWideArrow 
COMMENT                                   (CWideLine 
COMMENT                                    (CWidget 0 (3 #35# 0) 1 2 0 0 #278# 0 100) 
COMMENT                                    (LOGPEN 0 0 6723840) 1 
COMMENT                                    (LOGBRUSH 0 10079334 0) 0.835356 3.72332 
COMMENT                                    3.79853 1 0.082322) 0.8 1.8 0) 
COMMENT                             #355=(CLabel 
COMMENT                                   (CWidget 0 (0 0) 1 2 0 0 #274# 268730828 100) 
COMMENT                                   (LOGPEN 0 0 0) 1 
COMMENT                                   (LOGFONT 46 17 0 0 700 0 0 0 0 3 2 1 34 
COMMENT                                    "Arial") 1.74233 0.666667 0 "41BB" "@N" 1 
COMMENT                                   11.8295 -20.908 0 -10 2.39773 1.04901 #354#) 
COMMENT                             #356=(CWideArrow 
COMMENT                                   (CWideLine 
COMMENT                                    (CWidget 0 (3 #36# 0) 1 2 0 0 #278# 0 100) 
COMMENT                                    (LOGPEN 0 0 6723840) 1 
COMMENT                                    (LOGBRUSH 0 10079334 0) 0.835356 3.52098 
COMMENT                                    3.72332 1 0.082322) 0.8 1.8 0) 
COMMENT                             #357=(CLabel (CWidget 0 (0 0) 1 2 0 0 #274# 10 100) 
COMMENT                                   (LOGPEN 0 0 0) 1 
COMMENT                                   (LOGFONT 46 17 0 0 700 0 0 0 0 3 2 1 34 
COMMENT                                    "Arial") 1.74233 0.666667 0 "CD3Z" "@N" 1 
COMMENT                                   9.94402 -22.6503 0 -10 2.39773 1.04901 #356#) 
COMMENT                             #358=(CScratch 
COMMENT                                   (CWidget 0 (3 #37# 0) 1 2 0 0 #282# -986896 
COMMENT                                    100) (LOGPEN 0 8 13395507) 10 1 4.39302 1.9 
COMMENT                                   0.082322 1) 
COMMENT                             #359=(CLabel (CWidget 0 (0 0) 1 2 0 0 #274# 0 100) 
COMMENT                                   (LOGPEN 0 0 0) 1 
COMMENT                                   (LOGFONT 46 17 0 0 700 0 0 0 0 3 2 1 34 
COMMENT                                    "Arial") 1.74233 0.666667 0 
COMMENT                                   "GMCSF Leader Signal" "@N" 1 20.7549 -6.96932 
COMMENT                                   0 -10 9.61591 1.04901 #358#) 
COMMENT                             #360=(CScratch 
COMMENT                                   (CWidget 0 (3 #38# 0) 1 2 0 0 #282# -7028520 
COMMENT                                    100) (LOGPEN 0 8 13395507) 10 1 4.14591 1.9 
COMMENT                                   0.082322 1) 
COMMENT                             #361=(CLabel 
COMMENT                                   (CWidget 0 (0 0) 1 2 0 0 #274# 2082602632 100) 
COMMENT                                   (LOGPEN 0 0 0) 1 
COMMENT                                   (LOGFONT 46 17 0 0 700 0 0 0 0 3 2 1 34 
COMMENT                                    "Arial") 1.74233 0.666667 0 
COMMENT                                   "(GGGGS)3 linker" "@N" 1 18.0754 -10.454 0 
COMMENT                                   -10 7.31808 1.04901 #360#) 
COMMENT                             #362=(CWideArrow 
COMMENT                                   (CWideLine 
COMMENT                                    (CWidget 0 (3 #39# 0) 1 2 0 0 #278# 0 100) 
COMMENT                                    (LOGPEN 0 0 6723840) 1 
COMMENT                                    (LOGBRUSH 0 10079334 0) 0.835356 4.14591 
COMMENT                                    4.35363 1 0.082322) 0.8 1.8 0) 
COMMENT                             #363=(CLabel 
COMMENT                                   (CWidget 0 (0 0) 1 2 0 0 #274# 268730864 100) 
COMMENT                                   (LOGPEN 0 0 0) 1 
COMMENT                                   (LOGFONT 46 17 0 0 700 0 0 0 0 3 2 1 34 
COMMENT                                    "Arial") 1.74233 0.666667 0 "J1.F1 VH" "@N" 
COMMENT                                   1 17.1732 -8.71165 0 -10 3.97125 1.04901 
COMMENT                                   #362#) 
COMMENT                             #364=(CWideArrow 
COMMENT                                   (CWideLine 
COMMENT                                    (CWidget 0 (3 #40# 0) 1 2 0 0 #278# 0 100) 
COMMENT                                    (LOGPEN 0 0 6723840) 1 
COMMENT                                    (LOGBRUSH 0 10079334 0) 0.835356 3.92746 
COMMENT                                    4.11905 1 0.082322) 0.8 1.8 0) 
COMMENT                             #365=(CLabel 
COMMENT                                   (CWidget 0 (0 0) 1 2 0 0 #274# 268730828 100) 
COMMENT                                   (LOGPEN 0 0 0) 1 
COMMENT                                   (LOGFONT 46 17 0 0 700 0 0 0 0 3 2 1 34 
COMMENT                                    "Arial") 1.74233 0.666667 0 "J1.F1 VL" "@N" 
COMMENT                                   1 15.286 -12.1963 0 -10 3.89632 1.04901 #364#)) 
COMMENT                            (CObjectList)) 
COMMENT                      #366=(CGroupWidget 
COMMENT                            (CWidget 25 (7 25 0) 1 2 0 0 Nil -879 100) 
COMMENT                            (CObjectList 
COMMENT                             #367=(CLine 
COMMENT                                   (CWidget 0 (3 #19# 0) 1 2 0 0 #293# 636 100) 
COMMENT                                   (LOGPEN 0 6 32768) 8 0.835356 1.82346 1.89628) 
COMMENT                             #368=(CLabel 
COMMENT                                   (CWidget 0 (0 0) 1 2 0 0 #274# 268730864 100) 
COMMENT                                   (LOGPEN 0 0 0) 1 
COMMENT                                   (LOGFONT 46 17 0 0 700 0 0 0 0 3 2 1 34 
COMMENT                                    "Arial") 1.74233 0.666667 0 
COMMENT                                   "SV40 poly(A) signal" "@N" 1 -20.3396 
COMMENT                                   -5.22699 0 -10 8.467 1.04901 #367#)) 
COMMENT                            (CObjectList)) 
COMMENT                      #369=(CGroupWidget 
COMMENT                            (CWidget 30 (7 30 0) 1 2 0 0 Nil -1572 100) 
COMMENT                            (CObjectList 
COMMENT                             #370=(CWideArrow 
COMMENT                                   (CWideLine 
COMMENT                                    (CWidget 0 (3 #6# 0) 1 2 0 0 #290# 0 100) 
COMMENT                                    (LOGPEN 0 0 0) 1 (LOGBRUSH 0 128 0) 1 
COMMENT                                    6.13769 6.27319 1 0.082322) 0.8 1.8 0) 
COMMENT                             #371=(CLabel 
COMMENT                                   (CWidget 0 (0 0) 1 2 0 0 #274# -571201518 100) 
COMMENT                                   (LOGPEN 0 0 0) 1 
COMMENT                                   (LOGFONT 46 17 0 0 700 0 0 0 0 3 2 1 34 
COMMENT                                    "Arial") 1.74233 0.666667 0 "RSV promoter" 
COMMENT                                   "@N" 1 5.1259 17.4233 0 -10 6.09424 1.04901 
COMMENT                                   #370#) 
COMMENT                             #372=(CWideArrow 
COMMENT                                   (CWideLine 
COMMENT                                    (CWidget 0 (3 #13# 0) 1 2 0 0 #290# 0 100) 
COMMENT                                    (LOGPEN 0 0 0) 1 (LOGBRUSH 0 128 0) 0.835356 
COMMENT                                    4.40973 5.11345 1 0.082322) 0.8 1.8 0) 
COMMENT                             #373=(CLabel 
COMMENT                                   (CWidget 0 (0 0) 1 2 0 0 #274# 268730828 100) 
COMMENT                                   (LOGPEN 0 0 0) 1 
COMMENT                                   (LOGFONT 46 17 0 0 700 0 0 0 0 3 2 1 34 
COMMENT                                    "Arial") 1.74233 0.666667 0 "EF-1a" "@N" 1 
COMMENT                                   18.0049 0 0 -10 2.57257 1.04901 #372#) 
COMMENT                             #374=(CPromoterArrow 
COMMENT                                   (CLine 
COMMENT                                    (CWidget 0 (3 #21# 0) 1 2 0 0 #296# 0 100) 
COMMENT                                    (LOGPEN 0 4 128) 6 0.835356 1.70707 1.79434) 
COMMENT                                   0.4 0.082322 0.915356 1.8 1.8 1) 
COMMENT                             #375=(CLabel 
COMMENT                                   (CWidget 0 (0 0) 1 2 0 0 #274# -571132284 100) 
COMMENT                                   (LOGPEN 0 0 0) 1 
COMMENT                                   (LOGFONT 46 17 0 0 700 0 0 0 0 3 2 1 34 
COMMENT                                    "Arial") 1.74233 0.666667 0 "T7 promoter" 
COMMENT                                   "@N" 1 -19.3644 -1.74233 0 -10 5.22007 
COMMENT                                   1.04901 #374#) 
COMMENT                             #376=(CWideArrow 
COMMENT                                   (CWideLine 
COMMENT                                    (CWidget 0 (3 #23# 0) 1 2 0 0 #290# 0 100) 
COMMENT                                    (LOGPEN 0 0 0) 1 (LOGBRUSH 0 128 0) 1 
COMMENT                                    1.24628 1.30895 1 0.082322) 0.8 1.8 0) 
COMMENT                             #377=(CLabel 
COMMENT                                   (CWidget 0 (0 0) 1 2 0 0 #274# 2082602612 100) 
COMMENT                                   (LOGPEN 0 0 0) 1 
COMMENT                                   (LOGFONT 46 17 0 0 700 0 0 0 0 3 2 1 34 
COMMENT                                    "Arial") 1.74233 0.666667 0 "AmpR promoter" 
COMMENT                                   "@N" 1 -19.6142 5.22699 0 -10 6.79358 1.04901 
COMMENT                                   #376#) 
COMMENT                             #378=(CPromoterArrow 
COMMENT                                   (CLine 
COMMENT                                    (CWidget 0 (3 #27# 0) 1 2 0 0 #296# 0 100) 
COMMENT                                    (LOGPEN 0 4 128) 6 1.16464 6.28247 0.0865473) 
COMMENT                                   0.4 0.082322 1.08464 1.8 1.8 0) 
COMMENT                             #379=(CLabel 
COMMENT                                   (CWidget 0 (0 0) 1 2 0 0 #274# 1819632485 100) 
COMMENT                                   (LOGPEN 0 0 0) 1 
COMMENT                                   (LOGFONT 46 17 0 0 700 0 0 0 0 3 2 1 34 
COMMENT                                    "Arial") 1.74233 0.666667 0 "lac promoter" 
COMMENT                                   "@N" 1 -4.38228 19.1656 0 -10 5.56974 1.04901 
COMMENT                                   #378#) 
COMMENT                             #380=(CPromoterArrow 
COMMENT                                   (CLine 
COMMENT                                    (CWidget 0 (3 #29# 0) 1 2 0 0 #296# 0 100) 
COMMENT                                    (LOGPEN 0 4 128) 6 1.32929 6.22338 0.0274564) 
COMMENT                                   0.4 0.082322 1.24929 1.8 1.8 0) 
COMMENT                             #381=(CLabel 
COMMENT                                   (CWidget 0 (0 0) 1 2 0 0 #274# -585619202 100) 
COMMENT                                   (LOGPEN 0 0 0) 1 
COMMENT                                   (LOGFONT 46 17 0 0 700 0 0 0 0 3 2 1 34 
COMMENT                                    "Arial") 1.74233 0.666667 0 "Promoter P 1" 
COMMENT                                   "@N" 1 4.03216 20.908 0 -10 5.74457 1.04901 
COMMENT                                   #380#) 
COMMENT                             #382=(CPromoterArrow 
COMMENT                                   (CLine 
COMMENT                                    (CWidget 0 (3 #30# 0) 1 2 0 0 #296# 0 100) 
COMMENT                                    (LOGPEN 0 4 128) 6 1.49393 6.22338 0.0274564) 
COMMENT                                   0.4 0.082322 1.41393 1.8 1.8 0) 
COMMENT                             #383=(CLabel 
COMMENT                                   (CWidget 0 (0 0) 1 2 0 0 #274# 63164728 100) 
COMMENT                                   (LOGPEN 0 0 0) 1 
COMMENT                                   (LOGFONT 46 17 0 0 700 0 0 0 0 3 2 1 34 
COMMENT                                    "Arial") 1.74233 0.666667 0 "T3 promoter" 
COMMENT                                   "@N" 1 3.83881 19.1656 0 -10 5.22007 1.04901 
COMMENT                                   #382#)) (CObjectList)) 
COMMENT                      #384=(CGroupWidget 
COMMENT                            (CWidget 31 (7 31 0) 1 2 0 0 Nil -251 100) 
COMMENT                            (CObjectList 
COMMENT                             #385=(CScratch 
COMMENT                                   (CWidget 0 (3 #26# 0) 1 2 0 0 #280# 100683016 
COMMENT                                    100) (LOGPEN 0 6 6723840) 8 1 0.108035 1.9 
COMMENT                                   0.082322 1) 
COMMENT                             #386=(CLabel 
COMMENT                                   (CWidget 0 (0 0) 1 2 0 0 #274# 740331055 100) 
COMMENT                                   (LOGPEN 0 0 0) 1 
COMMENT                                   (LOGFONT 46 17 0 0 700 0 0 0 0 3 2 1 34 
COMMENT                                    "Arial") 1.74233 0.666667 0 
COMMENT                                   "CAP binding site" "@N" 1 -10.1789 15.681 0 
COMMENT                                   -10 7.24316 1.04901 #385#) 
COMMENT                             #387=(CScratch 
COMMENT                                   (CWidget 0 (3 #28# 0) 1 2 0 0 #280# 100682816 
COMMENT                                    100) (LOGPEN 0 6 6723840) 8 1 0.0638659 1.9 
COMMENT                                   0.082322 1) 
COMMENT                             #388=(CLabel 
COMMENT                                   (CWidget 0 (0 0) 1 2 0 0 #274# 268730864 100) 
COMMENT                                   (LOGPEN 0 0 0) 1 
COMMENT                                   (LOGFONT 46 17 0 0 700 0 0 0 0 3 2 1 34 
COMMENT                                    "Arial") 1.74233 0.666667 0 "lac operator" 
COMMENT                                   "@N" 1 -4.49325 17.4233 0 -10 5.295 1.04901 
COMMENT                                   #387#)) (CObjectList)) 
COMMENT                      #389=(CGroupWidget 
COMMENT                            (CWidget 33 (7 33 0) 1 2 0 0 Nil -1088 100) 
COMMENT                            (CObjectList 
COMMENT                             #390=(CWideArrow 
COMMENT                                   (CWideLine 
COMMENT                                    (CWidget 0 (3 #20# 0) 1 2 0 0 #277# 0 100) 
COMMENT                                    (LOGPEN 0 0 3355545) 1 
COMMENT                                    (LOGBRUSH 0 6724095 0) 1 1.71901 1.80018 1 
COMMENT                                    0.082322) 0.8 1.8 0) 
COMMENT                             #391=(CLabel 
COMMENT                                   (CWidget 0 (0 0) 1 2 0 0 #274# -585811548 100) 
COMMENT                                   (LOGPEN 0 0 0) 1 
COMMENT                                   (LOGFONT 46 17 0 0 700 0 0 0 0 3 2 1 34 
COMMENT                                    "Arial") 1.74233 0.666667 0 "SV40 ori" "@N" 
COMMENT                                   1 -18.3069 -3.48466 0 -10 3.79641 1.04901 
COMMENT                                   #390#) 
COMMENT                             #392=(CWideArrow 
COMMENT                                   (CWideLine 
COMMENT                                    (CWidget 0 (3 #22# 0) 1 2 0 0 #277# 0 100) 
COMMENT                                    (LOGPEN 0 0 3355545) 1 
COMMENT                                    (LOGBRUSH 0 6724095 0) 1 1.32387 1.59605 1 
COMMENT                                    0.082322) 0.8 1.8 0) 
COMMENT                             #393=(CLabel 
COMMENT                                   (CWidget 0 (0 0) 1 2 0 0 #274# 268730864 100) 
COMMENT                                   (LOGPEN 0 0 0) 1 
COMMENT                                   (LOGFONT 46 17 0 0 700 0 0 0 0 3 2 1 34 
COMMENT                                    "Arial") 1.74233 0.666667 0 "f1 ori" "@N" 1 
COMMENT                                   -17.8311 1.74233 0 -10 2.29783 1.04901 #392#) 
COMMENT                             #394=(CWideArrow 
COMMENT                                   (CWideLine 
COMMENT                                    (CWidget 0 (3 #25# 0) 1 2 0 0 #277# 0 100) 
COMMENT                                    (LOGPEN 0 0 3355545) 1 
COMMENT                                    (LOGBRUSH 0 6724095 0) 1 0.279339 0.6309 1 
COMMENT                                    0.082322) 0.8 1.8 0) 
COMMENT                             #395=(CLabel (CWidget 0 (0 0) 1 2 0 0 #274# 0 100) 
COMMENT                                   (LOGPEN 0 0 0) 1 
COMMENT                                   (LOGFONT 46 17 0 0 700 0 0 0 0 3 2 1 34 
COMMENT                                    "Arial") 1.74233 0.666667 0 "ori" "@N" 1 
COMMENT                                   -10.5248 13.9386 0 -10 1.24882 1.04901 #394#)) 
COMMENT                            (CObjectList))) (CObjectList)) 
COMMENT               #396=(CGroupWidget (CWidget 11 (8 0) 1 2 0 0 Nil -321 100) 
COMMENT                     (CObjectList 
COMMENT                      #397=(CGroupWidget 
COMMENT                            (CWidget 1 (10 #0# 0) 1 2 0 0 Nil -181 100) 
COMMENT                            (CObjectList 
COMMENT                             #398=(CScratch 
COMMENT                                   (CWidget 1 (1 #0# 1) 1 2 0 0 #281# 101322536 
COMMENT                                    100) (LOGPEN 0 6 10053171) 8 1 4.39959 1.9 
COMMENT                                   0.082322 1) 
COMMENT                             #399=(CLabel 
COMMENT                                   (CWidget 0 (0 0) 1 2 0 0 #283# 101 100) 
COMMENT                                   (LOGPEN 0 0 153) 1 
COMMENT                                   (LOGFONT 38 14 0 0 400 0 0 0 0 3 2 1 18 
COMMENT                                    "Georgia") 1.74233 0.555556 128 
COMMENT                                   "{\\i Eco}RI (3140)" "@N (@S)" 5 18.6711 
COMMENT                                   -5.22699 0 -10 4.24599 0.799245 #398#)) 
COMMENT                            (CObjectList)) 
COMMENT                      #400=(CGroupWidget 
COMMENT                            (CWidget 1 (10 #1# 0) 1 2 0 0 Nil -112 100) 
COMMENT                            (CObjectList 
COMMENT                             #401=(CScratch 
COMMENT                                   (CWidget 1 (1 #1# 1) 1 2 0 0 #281# 100339376 
COMMENT                                    100) (LOGPEN 0 6 10053171) 8 1 3.52038 1.9 
COMMENT                                   0.082322 1) 
COMMENT                             #402=(CLabel (CWidget 0 (0 0) 1 2 0 0 #283# -1 100) 
COMMENT                                   (LOGPEN 0 0 153) 1 
COMMENT                                   (LOGFONT 38 14 0 0 400 0 0 0 0 3 2 1 18 
COMMENT                                    "Georgia") 1.74233 0.555556 128 
COMMENT                                   "{\\i Bsi}WI (4613)" "@N (@S)" 5 9.77671 
COMMENT                                   -24.3926 0 -10 4.14608 0.799245 #401#)) 
COMMENT                            (CObjectList)) 
COMMENT                      #403=(CGroupWidget 
COMMENT                            (CWidget 1 (10 #2# 0) 1 2 0 0 Nil -181 100) 
COMMENT                            (CObjectList 
COMMENT                             #404=(CScratch 
COMMENT                                   (CWidget 1 (1 #2# 1) 1 2 0 0 #281# 100681256 
COMMENT                                    100) (LOGPEN 0 6 10053171) 8 1 3.92626 1.9 
COMMENT                                   0.082322 1) 
COMMENT                             #405=(CLabel 
COMMENT                                   (CWidget 0 (0 0) 1 2 0 0 #283# 1077014685 100) 
COMMENT                                   (LOGPEN 0 0 153) 1 
COMMENT                                   (LOGFONT 38 14 0 0 400 0 0 0 0 3 2 1 18 
COMMENT                                    "Georgia") 1.74233 0.555556 128 
COMMENT                                   "{\\i Not}I (3933)" "@N (@S)" 5 14.8796 
COMMENT                                   -15.681 0 -10 3.72148 0.799245 #404#)) 
COMMENT                            (CObjectList)) 
COMMENT                      #406=(CGroupWidget 
COMMENT                            (CWidget 1 (10 #4# 0) 1 2 0 0 Nil -739 100) 
COMMENT                            (CObjectList 
COMMENT                             #407=(CScratch 
COMMENT                                   (CWidget 1 (1 #4# 1) 1 2 0 0 #281# -986896 
COMMENT                                    100) (LOGPEN 0 6 10053171) 8 1 2.48421 1.9 
COMMENT                                   0.082322 1) 
COMMENT                             #408=(CLabel 
COMMENT                                   (CWidget 0 (0 0) 1 2 0 0 #283# 857940026 100) 
COMMENT                                   (LOGPEN 0 0 153) 1 
COMMENT                                   (LOGFONT 38 14 0 0 400 0 0 0 0 3 2 1 18 
COMMENT                                    "Georgia") 1.74233 0.555556 128 
COMMENT                                   "{\\i Sal}I (6349)" "@N (@S)" 5 -13.0564 
COMMENT                                   -13.9386 0 -10 3.72148 0.799245 #407#)) 
COMMENT                            (CObjectList)) 
COMMENT                      #409=(CGroupWidget 
COMMENT                            (CWidget 1 (10 #5# 0) 1 2 0 0 Nil -1019 100) 
COMMENT                            (CObjectList 
COMMENT                             #410=(CScratch 
COMMENT                                   (CWidget 1 (1 #5# 1) 1 2 0 0 #281# 845 100) 
COMMENT                                   (LOGPEN 0 6 10053171) 8 1 3.14137 1.9 
COMMENT                                   0.082322 1) 
COMMENT                             #411=(CLabel (CWidget 0 (0 0) 1 2 0 0 #283# 0 100) 
COMMENT                                   (LOGPEN 0 0 153) 1 
COMMENT                                   (LOGFONT 38 14 0 0 400 0 0 0 0 3 2 1 18 
COMMENT                                    "Georgia") 1.74233 0.555556 128 
COMMENT                                   "{\\i Spe}I (5248)" "@N (@S)" 5 -3.11435 
COMMENT                                   -17.4233 0 -10 3.79641 0.799245 #410#)) 
COMMENT                            (CObjectList))) (CObjectList)) 
COMMENT               #412=(CGroupWidget (CWidget 14 (16 0) 1 2 0 0 Nil -949 100) 
COMMENT                     (CObjectList) (CObjectList)) 
COMMENT               #413=(CGroupWidget (CWidget 12 (0 0) 1 2 0 0 Nil -251 100) 
COMMENT                     (CObjectList) (CObjectList))) (CObjectList))) 
COMMENT       (CSeqView 10 10 (CObjectList) (CObList) 1 (CObList)) (CObList) 0 
COMMENT       (CStringList) 2081894036 2081894196 (CObList)))
FEATURES             Location/Qualifiers
     source          1..10510
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
                     /vntifkey="98"
     promoter        1..227
                     /vntifkey="30"
                     /label=RSV\promoter
                     /note="RSV promoter Rous sarcoma virus enhancer/promoter  color: #ffffff"
     LTR             228..408
                     /vntifkey="19"
                     /label=5'\LTR\(RU5)
                     /note="5' LTR truncated 5' long terminal repeat (LTR) from HIV-1  color: #ffe4c4"
     misc_feature    410..563
                     /vntifkey="21"
                     /label=SL123
                     /note="SL123 color: #a6acb3"
     misc_feature    455..580
                     /vntifkey="21"
                     /label=HIV-1\Psi
                     /note="HIV-1 Psi packaging signal of human immunodeficiency virus  type 1 color: #a6acb3"
     CDS             564..927
                     /codon_start=1
                     /vntifkey="4"
                     /label=SL4\(Gag)
                     /note="SL4 Psi Stem-Loop 4 (SL4) coded in Gag protein  color: #993366"
     misc_feature    1073..1306
                     /vntifkey="21"
                     /label=RRE
                     /note="RRE The Rev response element (RRE) of HIV-1 allows for  Rev-dependent mRNA export from the nucleus to the cytoplasm. color: #a6acb3"
     misc_feature    1802..1919
                     /vntifkey="21"
                     /label=cPPT/CTS
                     /note="cPPT/CTS central polypurine tract and central termination  sequence of HIV-1 color: #a6acb3"
     promoter        1944..3122
                     /vntifkey="30"
                     /label=EF-1a
                     /note="EF-1a color: #ffffff"
     misc_feature    4618..4685
                     /vntifkey="21"
                     /label=Spacer\for\EMCV\IRES
                     /note="Spacer for EMCV IRES From 11 pRLLSIN.CMV.cLRGAG.IRES.Zeo.WPRE.v2  color: #a6acb3"
     misc_feature    4686..5246
                     /vntifkey="21"
                     /label=EMCV\IRES
                     /note="EMCV IRES color: #a6acb3"
     misc_feature    6354..6942
                     /vntifkey="21"
                     /label=WPRE
                     /note="WPRE woodchuck hepatitis virus posttranscriptional  regulatory element color: #a6acb3"
     CDS             complement(6825..6836)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /vntifkey="4"
                     /label=Factor\Xa\site
                     /note="Factor Xa site color: #993366"
     LTR             7029..7262
                     /vntifkey="19"
                     /label=3'\LTR\(Delta-U3RU5)
                     /note="3' LTR self-inactivating 3' long terminal repeat (LTR) from HIV-1 color: #ffe4c4"
     polyA_signal    7334..7455
                     /vntifkey="25"
                     /label=SV40\poly(A)\signal
                     /note="SV40 poly SV40 polyadenylation signal  color: #a6acb3"
     rep_origin      7495..7630
                     /vntifkey="33"
                     /label=SV40\ori
                     /note="SV40 ori SV40 origin of replication  color: #ffff00"
     promoter        complement(7651..7669)
                     /vntifkey="30"
                     /label=T7\promoter
                     /note="T7 promoter promoter for bacteriophage T7 RNA polymerase  color: #ffffff; direction: LEFT"
     rep_origin      7837..8292
                     /direction=RIGHT
                     /vntifkey="33"
                     /label=f1\ori
                     /note="f1 ori f1 bacteriophage origin of replication; arrow  indicates direction of (+) strand synthesis color: #ffff00"
     promoter        8318..8422
                     /gene="bla"
                     /vntifkey="30"
                     /label=AmpR\promoter
                     /note="AmpR promoter color: #ffffff"
     CDS             8423..9283
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /vntifkey="4"
                     /label=AmpR
                     /note="AmpR confers resistance to ampicillin, carbenicillin, and related antibiotics color: #993366"
     rep_origin      9454..10042
                     /direction=RIGHT
                     /vntifkey="33"
                     /label=ori
                     /note="ori high-copy-number ColE1/pMB1/pBR322/pUC origin of  replication color: #ffff00"
     protein_bind    10330..10351
                     /bound_moiety="E. coli catabolite activator protein"
                     /vntifkey="31"
                     /label=CAP\binding\site
                     /note="CAP binding site CAP binding activates transcription in the presence  of cAMP. color: #31849b"
     promoter        10366..10396
                     /vntifkey="30"
                     /label=lac\promoter
                     /note="lac promoter promoter for the E. coli lac operon  color: #ffffff"
     protein_bind    10404..10420
                     /bound_moiety="lac repressor encoded by lacI"
                     /vntifkey="31"
                     /label=lac\operator
                     /note="lac operator The lac repressor binds to the lac operator to  inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG). color: #31849b"
     promoter        10465..1
                     /vntifkey="30"
                     /label=Promoter_P_1
                     /note="color: #ffffff"
     promoter        10465..10483
                     /vntifkey="30"
                     /label=T3\promoter
                     /note="T3 promoter promoter for bacteriophage T3 RNA polymerase  color: #ffffff"
     misc_feature    5253..6347
                     /vntifkey="21"
                     /label=GFP-ZEO
     misc_feature    3931..3939
                     /vntifkey="21"
                     /label=AAA\linker\(also\NotI)
     misc_feature    3940..4146
                     /vntifkey="21"
                     /label=CD8A
     misc_feature    4075..4146
                     /vntifkey="21"
                     /label=TM\region
     misc_feature    4147..4272
                     /vntifkey="21"
                     /label=41BB
     misc_feature    4273..4611
                     /vntifkey="21"
                     /label=CD3Z
     misc_feature    3151..3216
                     /vntifkey="21"
                     /label=GMCSF\Leader\Signal
     misc_feature    3565..3609
                     /vntifkey="21"
                     /label=(GGGGS)3\linker
     misc_feature    3217..3564
                     /vntifkey="21"
                     /label=J1.F1\VH
     misc_feature    3610..3930
                     /vntifkey="21"
                     /label=J1.F1\VL
BASE COUNT     2520 a      2707 c      2873 g      2410 t 
ORIGIN
        1 tgtagtctta tgcaatactc ttgtagtctt gcaacatggt aacgatgagt tagcaacatg 
       61 ccttacaagg agagaaaaag caccgtgcat gccgattggt ggaagtaagg tggtacgatc 
      121 gtgccttatt aggaaggcaa cagacgggtc tgacatggat tggacgaacc actgaattgc 
      181 cgcattgcag agatattgta tttaagtgcc tagctcgata cataaacggg tctctctggt 
      241 tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc 
      301 aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta 
      361 actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt ggcgcccgaa 
      421 cagggacttg aaagcgaaag ggaaaccaga ggagctctct cgacgcagga ctcggcttgc 
      481 tgaagcgcgc acggcaagag gcgaggggcg gcgactggtg agtacgccaa aaattttgac 
      541 tagcggaggc tagaaggaga gagatgggtg cgagagcgtc agtattaagc gggggagaat 
      601 tagatcgcga tgggaaaaaa ttcggttaag gccaggggga aagaaaaaat ataaattaaa 
      661 acatatagta tgggcaagca gggagctaga acgattcgca gttaatcctg gcctgttaga 
      721 aacatcagaa ggctgtagac aaatactggg acagctacaa ccatcccttc agacaggatc 
      781 agaagaactt agatcattat ataatacagt agcaaccctc tattgtgtgc atcaaaggat 
      841 agagataaaa gacaccaagg aagctttaga caagatagag gaagagcaaa acaaaagtaa 
      901 gaccaccgca cagcaagcgg cctctgatct tcagacctgg aggaggagat atgagggaca 
      961 attggagaag tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac 
     1021 ccaccaaggc aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt 
     1081 tgttccttgg gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga 
     1141 cggtacaggc cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg 
     1201 ctattgaggc gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg 
     1261 caagaatcct ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt 
     1321 gctctggaaa actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat 
     1381 ctctggaaca gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt 
     1441 acacaagctt aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac 
     1501 aagaattatt ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt 
     1561 ggctgtggta tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag 
     1621 tttttgctgt actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc 
     1681 agacccacct cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg 
     1741 gagagagaga cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcggttaa 
     1801 cttttaaaag aaaagggggg attggggggt acagtgcagg ggaaagaata gtagacataa 
     1861 tagcaacaga catacaaact aaagaattac aaaaacaaat tacaaaaatt caaaatttta 
     1921 tcgatcacga gactagcctc gagggctccg gtgcccgtca gtgggcagag cgcacatcgc 
     1981 ccacagtccc cgagaagttg gggggagggg tcggcaattg aaccggtgcc tagagaaggt 
     2041 ggcgcggggt aaactgggaa agtgatgtcg tgtactggct ccgccttttt cccgagggtg 
     2101 ggggagaacc gtatataagt gcagtagtcg ccgtgaacgt tctttttcgc aacgggtttg 
     2161 ccgccagaac acaggtaagt gccgtgtgtg gttcccgcgg gcctggcctc tttacgggtt 
     2221 atggcccttg cgtgccttga attacttcca cctggctgca gtacgtgatt cttgatcccg 
     2281 agcttcgggt tggaagtggg tgggagagtt cgaggccttg cgcttaagga gccccttcgc 
     2341 ctcgtgcttg agttgaggcc tggcctgggc gctggggccg ccgcgtgcga atctggtggc 
     2401 accttcgcgc ctgtctcgct gctttcgata agtctctagc catttaaaat ttttgatgac 
     2461 ctgctgcgac gctttttttc tggcaagata gtcttgtaaa tgcgggccaa gatctgcaca 
     2521 ctggtatttc ggtttttggg gccgcgggcg gcgacggggc ccgtgcgtcc cagcgcacat 
     2581 gttcggcgag gcggggcctg cgagcgcggc caccgagaat cggacggggg tagtctcaag 
     2641 ctggccggcc tgctctggtg cctggcctcg cgccgccgtg tatcgccccg ccctgggcgg 
     2701 caaggctggc ccggtcggca ccagttgcgt gagcggaaag atggccgctt cccggccctg 
     2761 ctgcagggag ctcaaaatgg aggacgcggc gctcgggaga gcgggcgggt gagtcaccca 
     2821 cacaaaggaa aagggccttt ccgtcctcag ccgtcgcttc atgtgactcc acggagtacc 
     2881 gggcgccgtc caggcacctc gattagttct cgagcttttg gagtacgtcg tctttaggtt 
     2941 ggggggaggg gttttatgcg atggagtttc cccacactga gtgggtggag actgaagtta 
     3001 ggccagcttg gcacttgatg taattctcct tggaatttgc cctttttgag tttggatctt 
     3061 ggttcattct caagcctcag acagtggttc aaagtttttt tcttccattt caggtgtcgt 
     3121 gaggaattag cttggtacga attcgccacc atgcttctcc tggtgacaag ccttctgctc 
     3181 tgtgagttac cacacccagc attcctcctg atcccagagg tgcagctgct ggagagcgga 
     3241 ggaggactgg tgcagccagg aggcagcctg aggctgtcct gcgccgcctc tggcttcacc 
     3301 tttagctcct acgcaatgtc ttgggtgcgc caggcacctg gcaagggact ggagtgggtg 
     3361 agcaccatct ccacatctgg cgactacacc acatatgccg attccgtgaa gggccggttc 
     3421 accatcagca gagacaactc caagaataca ctgtatctgc agatgaactc tctgcgggcc 
     3481 gaggacaccg ccgtgtacta ttgtgccaag tccacagcct actttgatta ttggggccag 
     3541 ggcaccctgg tgacagtgtc tagcggcggc ggaggcagcg gcggcggagg cagcggcggc 
     3601 ggaggcagcg atatccagat gacccagagc cctagctccc tgagcgcctc cgtgggcgac 
     3661 agggtgacca tcacatgcag agcctctcag agcatctcta gctacctgaa ctggtatcag 
     3721 cagaagcccg gcaaggcccc taagctgctg atctacgcag catccgccct gcagtctgga 
     3781 gtgccatccc ggttctccgg atctggaagc ggcaccgact ttaccctgac aatctcctct 
     3841 ctgcagccag aggatttcgc cacatactat tgtcagcagg cctactatga ccccaccaca 
     3901 tttggccagg gcacaaaggt ggagatcaag gcggccgcaa ccacgacgcc agcgccgcga 
     3961 ccaccaacac cggcgcccac catcgcgtcg cagcccctgt ccctgcgccc agaggcgtgc 
     4021 cggccagcgg cggggggcgc agtgcacacg agggggctgg acttcgcctg tgatatctac 
     4081 atctgggcgc ccttggccgg gacttgtggg gtccttctcc tgtcactggt tatcaccctt 
     4141 tactgcaaac ggggcagaaa gaaactcctg tatatattca aacaaccatt tatgagacca 
     4201 gtacaaacta ctcaagagga agatggctgt agctgccgat ttccagaaga agaagaagga 
     4261 ggatgtgaac tgagagtgaa gttcagcagg agcgcagacg cccccgcgta ccagcagggc 
     4321 cagaaccagc tctataacga gctcaatcta ggacgaagag aggagtacga tgttttggac 
     4381 aagagacgtg gccgggaccc tgagatgggg ggaaagccga gaaggaagaa ccctcaggaa 
     4441 ggcctgtaca atgaactgca gaaagataag atggcggagg cctacagtga gattgggatg 
     4501 aaaggcgagc gccggagggg caaggggcac gatggccttt accagggtct cagtacagcc 
     4561 accaaggaca cctacgacgc ccttcacatg caggccctgc cccctcgcta acgtacgccc 
     4621 gcgaaattaa tacgactcac tatagggaga ccacaacggt ttccctctag cgggatcaat 
     4681 tccgcccccc ccctaacgtt actggccgaa gccgcttgga ataaggccgg tgtgcgtttg 
     4741 tctatatgtt attttccacc atattgccgt cttttggcaa tgtgagggcc cggaaacctg 
     4801 gccctgtctt cttgacgagc attcctaggg gtctttcccc tctcgccaaa ggaatgcaag 
     4861 gtctgttgaa tgtcgtgaag gaagcagttc ctctggaagc ttcttgaaga caaacaacgt 
     4921 ctgtagcgac cctttgcagg cagcggaacc ccccacctgg cgacaggtgc ctctgcggcc 
     4981 aaaagccacg tgtataagat acacctgcaa aggcggcaca accccagtgc cacgttgtga 
     5041 gttggatagt tgtggaaaga gtcaaatggc tctcctcaag cgtattcaac aaggggctga 
     5101 aggatgccca gaaggtaccc cattgtatgg gatctgatct ggggcctcgg tgcacatgct 
     5161 ttacatgtgt ttagtcgagg ttaaaaaaac gtctaggccc cccgaaccac ggggacgtgg 
     5221 ttttcctttg aaaacacgat gataatacta gtatggtgag caagggcgag gagctgttca 
     5281 ccggggtggt gcccatcctg gtcgagctgg acggcgacgt aaacggccac aagttcagcg 
     5341 tgtccggcga gggcgagggc gatgccacct acggcaagct gaccctgaag ttcatctgca 
     5401 ccaccggcaa gctgcccgtg ccctggccca ccctcgtgac caccctgacc tacggcgtgc 
     5461 agtgcttcag ccgctacccc gaccacatga agcagcacga cttcttcaag tccgccatgc 
     5521 ccgaaggcta cgtccaggag cgcaccatct tcttcaagga cgacggcaac tacaagaccc 
     5581 gcgccgaggt gaagttcgag ggcgacaccc tggtgaaccg catcgagctg aagggcatcg 
     5641 acttcaagga ggacggcaac atcctggggc acaagctgga gtacaactac aacagccaca 
     5701 acgtctatat catggccgac aagcagaaga acggcatcaa ggtgaacttc aagatccgcc 
     5761 acaacatcga ggacggcagc gtgcagctcg ccgaccacta ccagcagaac acccccatcg 
     5821 gcgacggccc cgtgctgctg cccgacaacc actacctgag cacccagtcc gccctgagca 
     5881 aagaccccaa cgagaagcgc gatcacatgg tcctgctgga gttcgtgacc gccgccggga 
     5941 tcactctcgg catggacgag ctgtacaagg gcatggccaa gttgaccagt gccgttccgg 
     6001 tgctcaccgc gcgcgacgtc gccggagcgg tcgagttctg gaccgaccgg ctcgggttct 
     6061 cccgggactt cgtggaggac gacttcgccg gtgtggtccg ggacgacgtg accctgttca 
     6121 tcagcgcggt ccaggaccag gtggtgccgg acaacaccct ggcctgggtg tgggtgcgcg 
     6181 gcctggacga gctgtacgcc gagtggtcgg aggtcgtgtc cacgaacttc cgggacgcct 
     6241 ccgggccggc catgaccgag atcggcgagc agccgtgggg gcgggagttc gccctgcgcg 
     6301 acccggccgg caactgcgtg cacttcgtgg ccgaggagca ggactgagtc gacaatcaac 
     6361 ctctggatta caaaatttgt gaaagattga ctggtattct taactatgtt gctcctttta 
     6421 cgctatgtgg atacgctgct ttaatgcctt tgtatcatgc tattgcttcc cgtatggctt 
     6481 tcattttctc ctccttgtat aaatcctggt tgctgtctct ttatgaggag ttgtggcccg 
     6541 ttgtcaggca acgtggcgtg gtgtgcactg tgtttgctga cgcaaccccc actggttggg 
     6601 gcattgccac cacctgtcag ctcctttccg ggactttcgc tttccccctc cctattgcca 
     6661 cggcggaact catcgccgcc tgccttgccc gctgctggac aggggctcgg ctgttgggca 
     6721 ctgacaattc cgtggtgttg tcggggaagc tgacgtcctt tccatggctg ctcgcctgtg 
     6781 ttgccacctg gattctgcgc gggacgtcct tctgctacgt cccttcggcc ctcaatccag 
     6841 cggaccttcc ttcccgcggc ctgctgccgg ctctgcggcc tcttccgcgt cttcgccttc 
     6901 gccctcagac gagtcggatc tccctttggg ccgcctcccc gcctgtaatt cgagctcggt 
     6961 acctttaaga ccaatgactt acaaggcagc tgtagatctt agccactttt taaaagaaaa 
     7021 ggggggactg gaagggctaa ttcactccca acgaagacaa gatctgcttt ttgcttgtac 
     7081 tgggtctctc tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc 
     7141 actgcttaag cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt 
     7201 gtgtgactct ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag 
     7261 cagtagtagt tcatgtcatc ttattattca gtatttataa cttgcaaaga aatgaatatc 
     7321 agagagtgag aggaacttgt ttattgcagc ttataatggt tacaaataaa gcaatagcat 
     7381 cacaaatttc acaaataaag catttttttc actgcattct agttgtggtt tgtccaaact 
     7441 catcaatgta tcttatcatg tctggctcta gctatcccgc ccctaactcc gcccatcccg 
     7501 cccctaactc cgcccagttc cgcccattct ccgccccatg gctgactaat tttttttatt 
     7561 tatgcagagg ccgaggccgc ctcggcctct gagctattcc agaagtagtg aggaggcttt 
     7621 tttggaggcc tagggacgta cccaattcgc cctatagtga gtcgtattac gcgcgctcac 
     7681 tggccgtcgt tttacaacgt cgtgactggg aaaaccctgg cgttacccaa cttaatcgcc 
     7741 ttgcagcaca tccccctttc gccagctggc gtaatagcga agaggcccgc accgatcgcc 
     7801 cttcccaaca gttgcgcagc ctgaatggcg aatgggacgc gccctgtagc ggcgcattaa 
     7861 gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac acttgccagc gccctagcgc 
     7921 ccgctccttt cgctttcttc ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag 
     7981 ctctaaatcg ggggctccct ttagggttcc gatttagtgc tttacggcac ctcgacccca 
     8041 aaaaacttga ttagggtgat ggttcacgta gtgggccatc gccctgatag acggtttttc 
     8101 gccctttgac gttggagtcc acgttcttta atagtggact cttgttccaa actggaacaa 
     8161 cactcaaccc tatctcggtc tattcttttg atttataagg gattttgccg atttcggcct 
     8221 attggttaaa aaatgagctg atttaacaaa aatttaacgc gaattttaac aaaatattaa 
     8281 cgcttacaat ttaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt 
     8341 tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca 
     8401 ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt 
     8461 ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga 
     8521 tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa 
     8581 gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct 
     8641 gctatgtggc gcggtattat cccgtattga cgccgggcaa gagcaactcg gtcgccgcat 
     8701 acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga 
     8761 tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc 
     8821 caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat 
     8881 gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa 
     8941 cgacgagcgt gacaccacga tgcctgtagc aatggcaaca acgttgcgca aactattaac 
     9001 tggcgaacta cttactctag cttcccggca acaattaata gactggatgg aggcggataa 
     9061 agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc 
     9121 tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc 
     9181 ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag 
     9241 acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag accaagttta 
     9301 ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa 
     9361 gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc 
     9421 gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat 
     9481 ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga 
     9541 gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt 
     9601 tcttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata 
     9661 cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac 
     9721 cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg 
     9781 ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg 
     9841 tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag 
     9901 cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct 
     9961 ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc 
    10021 aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt 
    10081 ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg 
    10141 tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga 
    10201 gtcagtgagc gaggaagcgg aagagcgccc aatacgcaaa ccgcctctcc ccgcgcgttg 
    10261 gccgattcat taatgcagct ggcacgacag gtttcccgac tggaaagcgg gcagtgagcg 
    10321 caacgcaatt aatgtgagtt agctcactca ttaggcaccc caggctttac actttatgct 
    10381 tccggctcgt atgttgtgtg gaattgtgag cggataacaa tttcacacag gaaacagcta 
    10441 tgaccatgat tacgccaagc gcgcaattaa ccctcactaa agggaacaaa agctggagct 
    10501 gcaagcttaa 
//
