LOCUS       pRRL.SIN.EF1A.JAG1-F1.GS.CAR-3G       10645 bp    DNA     circular SYN  3-NOV-2022
DEFINITION  3rd generation lentiviral backbone.
ACCESSION   .
VERSION     .
KEYWORDS    S7_pRRLSIN; EF1a; CRE; IRES; Puro; WPRE; v2.
SOURCE      synthetic DNA construct.
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9606)
  TITLE     Trono Lab Misc Plasmids
REFERENCE   2  (bases 1 to 9606)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Tuesday, Jun 20, 2017 from SnapGene Console 4.0.0a8 
            http://www.snapgene.com
REFERENCE   3  (bases 1 to 9606)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported sexta-feira, 1 de fev de 2019 from SnapGene 1.1.3
            http://www.snapgene.com
COMMENT     This file is created by Vector NTI
            http://www.invitrogen.com/
COMMENT     ORIGDB|GenBank
COMMENT     VNTDATE|-14641157|
COMMENT     VNTDBDATE|-14641157|
COMMENT     LSOWNER|
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COMMENT     VNTAUTHORNAME|Ana Filipa Rodrigues|
COMMENT     VNTAUTHOREML|afrodrigues@ibet.pt|
COMMENT     VNTOAUTHORNAME|UNKNOWN|
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COMMENT                          "Foreign object. Author: Ana Filipa Rodrigues. Original author: UNKNOWN" 
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COMMENT                          "Keywords: S7_pRRLSIN; EF1a; CRE; IRES; Puro; WPRE; v2" 
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FEATURES             Location/Qualifiers
     source          1..10645
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
                     /vntifkey="98"
     promoter        1..227
                     /vntifkey="30"
                     /label=RSV\promoter
                     /note="RSV promoter Rous sarcoma virus enhancer/promoter  color: #ffffff"
     LTR             228..408
                     /vntifkey="19"
                     /label=5'\LTR\(RU5)
                     /note="5' LTR truncated 5' long terminal repeat (LTR) from HIV-1  color: #ffe4c4"
     misc_feature    410..563
                     /vntifkey="21"
                     /label=SL123
                     /note="SL123 color: #a6acb3"
     misc_feature    455..580
                     /vntifkey="21"
                     /label=HIV-1\Psi
                     /note="HIV-1 Psi packaging signal of human immunodeficiency virus  type 1 color: #a6acb3"
     CDS             564..927
                     /codon_start=1
                     /vntifkey="4"
                     /label=SL4\(Gag)
                     /note="SL4 Psi Stem-Loop 4 (SL4) coded in Gag protein  color: #993366"
     misc_feature    1073..1306
                     /vntifkey="21"
                     /label=RRE
                     /note="RRE The Rev response element (RRE) of HIV-1 allows for  Rev-dependent mRNA export from the nucleus to the cytoplasm. color: #a6acb3"
     misc_feature    1802..1919
                     /vntifkey="21"
                     /label=cPPT/CTS
                     /note="cPPT/CTS central polypurine tract and central termination  sequence of HIV-1 color: #a6acb3"
     promoter        1944..3122
                     /vntifkey="30"
                     /label=EF-1a
                     /note="EF-1a color: #ffffff"
     misc_feature    4753..4820
                     /vntifkey="21"
                     /label=Spacer\for\EMCV\IRES
                     /note="Spacer for EMCV IRES From 11 pRLLSIN.CMV.cLRGAG.IRES.Zeo.WPRE.v2  color: #a6acb3"
     misc_feature    4821..5381
                     /vntifkey="21"
                     /label=EMCV\IRES
                     /note="EMCV IRES color: #a6acb3"
     misc_feature    6489..7077
                     /vntifkey="21"
                     /label=WPRE
                     /note="WPRE woodchuck hepatitis virus posttranscriptional  regulatory element color: #a6acb3"
     CDS             complement(6960..6971)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /vntifkey="4"
                     /label=Factor\Xa\site
                     /note="Factor Xa site color: #993366"
     LTR             7164..7397
                     /vntifkey="19"
                     /label=3'\LTR\(Delta-U3RU5)
                     /note="3' LTR self-inactivating 3' long terminal repeat (LTR) from HIV-1 color: #ffe4c4"
     polyA_signal    7469..7590
                     /vntifkey="25"
                     /label=SV40\poly(A)\signal
                     /note="SV40 poly SV40 polyadenylation signal  color: #a6acb3"
     rep_origin      7630..7765
                     /vntifkey="33"
                     /label=SV40\ori
                     /note="SV40 ori SV40 origin of replication  color: #ffff00"
     promoter        complement(7786..7804)
                     /vntifkey="30"
                     /label=T7\promoter
                     /note="T7 promoter promoter for bacteriophage T7 RNA polymerase  color: #ffffff; direction: LEFT"
     rep_origin      7972..8427
                     /direction=RIGHT
                     /vntifkey="33"
                     /label=f1\ori
                     /note="f1 ori f1 bacteriophage origin of replication; arrow  indicates direction of (+) strand synthesis color: #ffff00"
     promoter        8453..8557
                     /gene="bla"
                     /vntifkey="30"
                     /label=AmpR\promoter
                     /note="AmpR promoter color: #ffffff"
     CDS             8558..9418
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /vntifkey="4"
                     /label=AmpR
                     /note="AmpR confers resistance to ampicillin, carbenicillin, and related antibiotics color: #993366"
     rep_origin      9589..10177
                     /direction=RIGHT
                     /vntifkey="33"
                     /label=ori
                     /note="ori high-copy-number ColE1/pMB1/pBR322/pUC origin of  replication color: #ffff00"
     protein_bind    10465..10486
                     /bound_moiety="E. coli catabolite activator protein"
                     /vntifkey="31"
                     /label=CAP\binding\site
                     /note="CAP binding site CAP binding activates transcription in the presence  of cAMP. color: #31849b"
     promoter        10501..10531
                     /vntifkey="30"
                     /label=lac\promoter
                     /note="lac promoter promoter for the E. coli lac operon  color: #ffffff"
     protein_bind    10539..10555
                     /bound_moiety="lac repressor encoded by lacI"
                     /vntifkey="31"
                     /label=lac\operator
                     /note="lac operator The lac repressor binds to the lac operator to  inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG). color: #31849b"
     promoter        10600..1
                     /vntifkey="30"
                     /label=Promoter_P_1
                     /note="color: #ffffff"
     promoter        10600..10618
                     /vntifkey="30"
                     /label=T3\promoter
                     /note="T3 promoter promoter for bacteriophage T3 RNA polymerase  color: #ffffff"
     misc_feature    5388..6482
                     /vntifkey="21"
                     /label=GFP-ZEO
     misc_feature    3940..4074
                     /vntifkey="21"
                     /label=CD8A\hinge
     misc_feature    4075..4156
                     /vntifkey="21"
                     /label=CD28\TM
     misc_feature    4157..4281
                     /vntifkey="21"
                     /label=CD28\SM
     misc_feature    4282..4407
                     /vntifkey="21"
                     /label=41BB
     misc_feature    4408..4746
                     /vntifkey="21"
                     /label=CD3Z
     misc_feature    3151..3216
                     /vntifkey="21"
                     /label=GMCSF\Leader\Signal
     misc_feature    3565..3609
                     /vntifkey="21"
                     /label=(GGGGS)3\linker
     misc_feature    3931..3939
                     /vntifkey="21"
                     /label=AAA\linker\(also\NotI)
     misc_feature    3217..3564
                     /vntifkey="21"
                     /label=J1.F1\VH
     misc_feature    3610..3930
                     /vntifkey="21"
                     /label=J1.F1\VL
BASE COUNT     2551 a      2745 c      2913 g      2436 t 
ORIGIN
        1 tgtagtctta tgcaatactc ttgtagtctt gcaacatggt aacgatgagt tagcaacatg 
       61 ccttacaagg agagaaaaag caccgtgcat gccgattggt ggaagtaagg tggtacgatc 
      121 gtgccttatt aggaaggcaa cagacgggtc tgacatggat tggacgaacc actgaattgc 
      181 cgcattgcag agatattgta tttaagtgcc tagctcgata cataaacggg tctctctggt 
      241 tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc 
      301 aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta 
      361 actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt ggcgcccgaa 
      421 cagggacttg aaagcgaaag ggaaaccaga ggagctctct cgacgcagga ctcggcttgc 
      481 tgaagcgcgc acggcaagag gcgaggggcg gcgactggtg agtacgccaa aaattttgac 
      541 tagcggaggc tagaaggaga gagatgggtg cgagagcgtc agtattaagc gggggagaat 
      601 tagatcgcga tgggaaaaaa ttcggttaag gccaggggga aagaaaaaat ataaattaaa 
      661 acatatagta tgggcaagca gggagctaga acgattcgca gttaatcctg gcctgttaga 
      721 aacatcagaa ggctgtagac aaatactggg acagctacaa ccatcccttc agacaggatc 
      781 agaagaactt agatcattat ataatacagt agcaaccctc tattgtgtgc atcaaaggat 
      841 agagataaaa gacaccaagg aagctttaga caagatagag gaagagcaaa acaaaagtaa 
      901 gaccaccgca cagcaagcgg cctctgatct tcagacctgg aggaggagat atgagggaca 
      961 attggagaag tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac 
     1021 ccaccaaggc aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt 
     1081 tgttccttgg gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga 
     1141 cggtacaggc cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg 
     1201 ctattgaggc gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg 
     1261 caagaatcct ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt 
     1321 gctctggaaa actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat 
     1381 ctctggaaca gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt 
     1441 acacaagctt aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac 
     1501 aagaattatt ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt 
     1561 ggctgtggta tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag 
     1621 tttttgctgt actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc 
     1681 agacccacct cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg 
     1741 gagagagaga cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcggttaa 
     1801 cttttaaaag aaaagggggg attggggggt acagtgcagg ggaaagaata gtagacataa 
     1861 tagcaacaga catacaaact aaagaattac aaaaacaaat tacaaaaatt caaaatttta 
     1921 tcgatcacga gactagcctc gagggctccg gtgcccgtca gtgggcagag cgcacatcgc 
     1981 ccacagtccc cgagaagttg gggggagggg tcggcaattg aaccggtgcc tagagaaggt 
     2041 ggcgcggggt aaactgggaa agtgatgtcg tgtactggct ccgccttttt cccgagggtg 
     2101 ggggagaacc gtatataagt gcagtagtcg ccgtgaacgt tctttttcgc aacgggtttg 
     2161 ccgccagaac acaggtaagt gccgtgtgtg gttcccgcgg gcctggcctc tttacgggtt 
     2221 atggcccttg cgtgccttga attacttcca cctggctgca gtacgtgatt cttgatcccg 
     2281 agcttcgggt tggaagtggg tgggagagtt cgaggccttg cgcttaagga gccccttcgc 
     2341 ctcgtgcttg agttgaggcc tggcctgggc gctggggccg ccgcgtgcga atctggtggc 
     2401 accttcgcgc ctgtctcgct gctttcgata agtctctagc catttaaaat ttttgatgac 
     2461 ctgctgcgac gctttttttc tggcaagata gtcttgtaaa tgcgggccaa gatctgcaca 
     2521 ctggtatttc ggtttttggg gccgcgggcg gcgacggggc ccgtgcgtcc cagcgcacat 
     2581 gttcggcgag gcggggcctg cgagcgcggc caccgagaat cggacggggg tagtctcaag 
     2641 ctggccggcc tgctctggtg cctggcctcg cgccgccgtg tatcgccccg ccctgggcgg 
     2701 caaggctggc ccggtcggca ccagttgcgt gagcggaaag atggccgctt cccggccctg 
     2761 ctgcagggag ctcaaaatgg aggacgcggc gctcgggaga gcgggcgggt gagtcaccca 
     2821 cacaaaggaa aagggccttt ccgtcctcag ccgtcgcttc atgtgactcc acggagtacc 
     2881 gggcgccgtc caggcacctc gattagttct cgagcttttg gagtacgtcg tctttaggtt 
     2941 ggggggaggg gttttatgcg atggagtttc cccacactga gtgggtggag actgaagtta 
     3001 ggccagcttg gcacttgatg taattctcct tggaatttgc cctttttgag tttggatctt 
     3061 ggttcattct caagcctcag acagtggttc aaagtttttt tcttccattt caggtgtcgt 
     3121 gaggaattag cttggtacga attcgccacc atgcttctcc tggtgacaag ccttctgctc 
     3181 tgtgagttac cacacccagc attcctcctg atcccagagg tgcagctgct ggagagcgga 
     3241 ggaggactgg tgcagccagg aggcagcctg aggctgtcct gcgccgcctc tggcttcacc 
     3301 tttagctcct acgcaatgtc ttgggtgcgc caggcacctg gcaagggact ggagtgggtg 
     3361 agcaccatct ccacatctgg cgactacacc acatatgccg attccgtgaa gggccggttc 
     3421 accatcagca gagacaactc caagaataca ctgtatctgc agatgaactc tctgcgggcc 
     3481 gaggacaccg ccgtgtacta ttgtgccaag tccacagcct actttgatta ttggggccag 
     3541 ggcaccctgg tgacagtgtc tagcggcggc ggaggcagcg gcggcggagg cagcggcggc 
     3601 ggaggcagcg atatccagat gacccagagc cctagctccc tgagcgcctc cgtgggcgac 
     3661 agggtgacca tcacatgcag agcctctcag agcatctcta gctacctgaa ctggtatcag 
     3721 cagaagcccg gcaaggcccc taagctgctg atctacgcag catccgccct gcagtctgga 
     3781 gtgccatccc ggttctccgg atctggaagc ggcaccgact ttaccctgac aatctcctct 
     3841 ctgcagccag aggatttcgc cacatactat tgtcagcagg cctactatga ccccaccaca 
     3901 tttggccagg gcacaaaggt ggagatcaag gcggccgcaa ccacgacgcc agcgccgcga 
     3961 ccaccaacac cggcgcccac catcgcgtcg cagcccctgt ccctgcgccc agaggcgtgc 
     4021 cggccagcgg cggggggcgc agtgcacacg agggggctgg acttcgcctg tgatttttgg 
     4081 gtgctggtgg tggttggggg agtcctggct tgctatagct tgctagtaac agtggccttt 
     4141 attattttct gggtgaggag taagaggagc aggctcctgc acagtgacta catgaacatg 
     4201 actccccgcc gccccgggcc cacccgcaag cattaccagc cctatgcccc accacgcgac 
     4261 ttcgcagcct atcgctccag aaaacggggc agaaagaaac tcctgtatat attcaaacaa 
     4321 ccatttatga gaccagtaca aactactcaa gaggaagatg gctgtagctg ccgatttcca 
     4381 gaagaagaag aaggaggatg tgaactgaga gtgaagttca gcaggagcgc agacgccccc 
     4441 gcgtaccagc agggccagaa ccagctctat aacgagctca atctaggacg aagagaggag 
     4501 tacgatgttt tggacaagag acgtggccgg gaccctgaga tggggggaaa gccgagaagg 
     4561 aagaaccctc aggaaggcct gtacaatgaa ctgcagaaag ataagatggc ggaggcctac 
     4621 agtgagattg ggatgaaagg cgagcgccgg aggggcaagg ggcacgatgg cctttaccag 
     4681 ggtctcagta cagccaccaa ggacacctac gacgcccttc acatgcaggc cctgccccct 
     4741 cgctaacgta cgcccgcgaa attaatacga ctcactatag ggagaccaca acggtttccc 
     4801 tctagcggga tcaattccgc ccccccccta acgttactgg ccgaagccgc ttggaataag 
     4861 gccggtgtgc gtttgtctat atgttatttt ccaccatatt gccgtctttt ggcaatgtga 
     4921 gggcccggaa acctggccct gtcttcttga cgagcattcc taggggtctt tcccctctcg 
     4981 ccaaaggaat gcaaggtctg ttgaatgtcg tgaaggaagc agttcctctg gaagcttctt 
     5041 gaagacaaac aacgtctgta gcgacccttt gcaggcagcg gaacccccca cctggcgaca 
     5101 ggtgcctctg cggccaaaag ccacgtgtat aagatacacc tgcaaaggcg gcacaacccc 
     5161 agtgccacgt tgtgagttgg atagttgtgg aaagagtcaa atggctctcc tcaagcgtat 
     5221 tcaacaaggg gctgaaggat gcccagaagg taccccattg tatgggatct gatctggggc 
     5281 ctcggtgcac atgctttaca tgtgtttagt cgaggttaaa aaaacgtcta ggccccccga 
     5341 accacgggga cgtggttttc ctttgaaaac acgatgataa tactagtatg gtgagcaagg 
     5401 gcgaggagct gttcaccggg gtggtgccca tcctggtcga gctggacggc gacgtaaacg 
     5461 gccacaagtt cagcgtgtcc ggcgagggcg agggcgatgc cacctacggc aagctgaccc 
     5521 tgaagttcat ctgcaccacc ggcaagctgc ccgtgccctg gcccaccctc gtgaccaccc 
     5581 tgacctacgg cgtgcagtgc ttcagccgct accccgacca catgaagcag cacgacttct 
     5641 tcaagtccgc catgcccgaa ggctacgtcc aggagcgcac catcttcttc aaggacgacg 
     5701 gcaactacaa gacccgcgcc gaggtgaagt tcgagggcga caccctggtg aaccgcatcg 
     5761 agctgaaggg catcgacttc aaggaggacg gcaacatcct ggggcacaag ctggagtaca 
     5821 actacaacag ccacaacgtc tatatcatgg ccgacaagca gaagaacggc atcaaggtga 
     5881 acttcaagat ccgccacaac atcgaggacg gcagcgtgca gctcgccgac cactaccagc 
     5941 agaacacccc catcggcgac ggccccgtgc tgctgcccga caaccactac ctgagcaccc 
     6001 agtccgccct gagcaaagac cccaacgaga agcgcgatca catggtcctg ctggagttcg 
     6061 tgaccgccgc cgggatcact ctcggcatgg acgagctgta caagggcatg gccaagttga 
     6121 ccagtgccgt tccggtgctc accgcgcgcg acgtcgccgg agcggtcgag ttctggaccg 
     6181 accggctcgg gttctcccgg gacttcgtgg aggacgactt cgccggtgtg gtccgggacg 
     6241 acgtgaccct gttcatcagc gcggtccagg accaggtggt gccggacaac accctggcct 
     6301 gggtgtgggt gcgcggcctg gacgagctgt acgccgagtg gtcggaggtc gtgtccacga 
     6361 acttccggga cgcctccggg ccggccatga ccgagatcgg cgagcagccg tgggggcggg 
     6421 agttcgccct gcgcgacccg gccggcaact gcgtgcactt cgtggccgag gagcaggact 
     6481 gagtcgacaa tcaacctctg gattacaaaa tttgtgaaag attgactggt attcttaact 
     6541 atgttgctcc ttttacgcta tgtggatacg ctgctttaat gcctttgtat catgctattg 
     6601 cttcccgtat ggctttcatt ttctcctcct tgtataaatc ctggttgctg tctctttatg 
     6661 aggagttgtg gcccgttgtc aggcaacgtg gcgtggtgtg cactgtgttt gctgacgcaa 
     6721 cccccactgg ttggggcatt gccaccacct gtcagctcct ttccgggact ttcgctttcc 
     6781 ccctccctat tgccacggcg gaactcatcg ccgcctgcct tgcccgctgc tggacagggg 
     6841 ctcggctgtt gggcactgac aattccgtgg tgttgtcggg gaagctgacg tcctttccat 
     6901 ggctgctcgc ctgtgttgcc acctggattc tgcgcgggac gtccttctgc tacgtccctt 
     6961 cggccctcaa tccagcggac cttccttccc gcggcctgct gccggctctg cggcctcttc 
     7021 cgcgtcttcg ccttcgccct cagacgagtc ggatctccct ttgggccgcc tccccgcctg 
     7081 taattcgagc tcggtacctt taagaccaat gacttacaag gcagctgtag atcttagcca 
     7141 ctttttaaaa gaaaaggggg gactggaagg gctaattcac tcccaacgaa gacaagatct 
     7201 gctttttgct tgtactgggt ctctctggtt agaccagatc tgagcctggg agctctctgg 
     7261 ctaactaggg aacccactgc ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt 
     7321 gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc ctcagaccct tttagtcagt 
     7381 gtggaaaatc tctagcagta gtagttcatg tcatcttatt attcagtatt tataacttgc 
     7441 aaagaaatga atatcagaga gtgagaggaa cttgtttatt gcagcttata atggttacaa 
     7501 ataaagcaat agcatcacaa atttcacaaa taaagcattt ttttcactgc attctagttg 
     7561 tggtttgtcc aaactcatca atgtatctta tcatgtctgg ctctagctat cccgccccta 
     7621 actccgccca tcccgcccct aactccgccc agttccgccc attctccgcc ccatggctga 
     7681 ctaatttttt ttatttatgc agaggccgag gccgcctcgg cctctgagct attccagaag 
     7741 tagtgaggag gcttttttgg aggcctaggg acgtacccaa ttcgccctat agtgagtcgt 
     7801 attacgcgcg ctcactggcc gtcgttttac aacgtcgtga ctgggaaaac cctggcgtta 
     7861 cccaacttaa tcgccttgca gcacatcccc ctttcgccag ctggcgtaat agcgaagagg 
     7921 cccgcaccga tcgcccttcc caacagttgc gcagcctgaa tggcgaatgg gacgcgccct 
     7981 gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg 
     8041 ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg 
     8101 gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt agtgctttac 
     8161 ggcacctcga ccccaaaaaa cttgattagg gtgatggttc acgtagtggg ccatcgccct 
     8221 gatagacggt ttttcgccct ttgacgttgg agtccacgtt ctttaatagt ggactcttgt 
     8281 tccaaactgg aacaacactc aaccctatct cggtctattc ttttgattta taagggattt 
     8341 tgccgatttc ggcctattgg ttaaaaaatg agctgattta acaaaaattt aacgcgaatt 
     8401 ttaacaaaat attaacgctt acaatttagg tggcactttt cggggaaatg tgcgcggaac 
     8461 ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc 
     8521 ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt 
     8581 cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct 
     8641 ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga 
     8701 tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag 
     8761 cacttttaaa gttctgctat gtggcgcggt attatcccgt attgacgccg ggcaagagca 
     8821 actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga 
     8881 aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag 
     8941 tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc 
     9001 ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa 
     9061 tgaagccata ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg caacaacgtt 
     9121 gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg 
     9181 gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt 
     9241 tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg 
     9301 gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat 
     9361 ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact 
     9421 gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa 
     9481 aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt 
     9541 ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt 
     9601 ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg 
     9661 tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca 
     9721 gataccaaat actgttcttc tagtgtagcc gtagttaggc caccacttca agaactctgt 
     9781 agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga 
     9841 taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc 
     9901 gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact 
     9961 gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga 
    10021 caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg 
    10081 aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt 
    10141 tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt 
    10201 acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga 
    10261 ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac 
    10321 gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcccaatac gcaaaccgcc 
    10381 tctccccgcg cgttggccga ttcattaatg cagctggcac gacaggtttc ccgactggaa 
    10441 agcgggcagt gagcgcaacg caattaatgt gagttagctc actcattagg caccccaggc 
    10501 tttacacttt atgcttccgg ctcgtatgtt gtgtggaatt gtgagcggat aacaatttca 
    10561 cacaggaaac agctatgacc atgattacgc caagcgcgca attaaccctc actaaaggga 
    10621 acaaaagctg gagctgcaag cttaa 
//
