LOCUS       pRRL.SIN.EF1A.JAG1-B5.GS.CAR-2G       10510 bp    DNA     circular SYN  3-NOV-2022
DEFINITION  3rd generation lentiviral backbone.
ACCESSION   .
VERSION     .
KEYWORDS    S7_pRRLSIN; EF1a; CRE; IRES; Puro; WPRE; v2.
SOURCE      synthetic DNA construct.
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9606)
  TITLE     Trono Lab Misc Plasmids
REFERENCE   2  (bases 1 to 9606)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Tuesday, Jun 20, 2017 from SnapGene Console 4.0.0a8 
            http://www.snapgene.com
REFERENCE   3  (bases 1 to 9606)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported sexta-feira, 1 de fev de 2019 from SnapGene 1.1.3
            http://www.snapgene.com
COMMENT     This file is created by Vector NTI
            http://www.invitrogen.com/
COMMENT     ORIGDB|GenBank
COMMENT     VNTDATE|-14641153|
COMMENT     VNTDBDATE|-14641153|
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COMMENT     VNTAUTHORNAME|Ana Filipa Rodrigues|
COMMENT     VNTAUTHOREML|afrodrigues@ibet.pt|
COMMENT     VNTOAUTHORNAME|UNKNOWN|
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COMMENT                          "Keywords: S7_pRRLSIN; EF1a; CRE; IRES; Puro; WPRE; v2" 
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FEATURES             Location/Qualifiers
     source          1..10510
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
                     /vntifkey="98"
     promoter        1..227
                     /vntifkey="30"
                     /label=RSV\promoter
                     /note="RSV promoter Rous sarcoma virus enhancer/promoter  color: #ffffff"
     LTR             228..408
                     /vntifkey="19"
                     /label=5'\LTR\(RU5)
                     /note="5' LTR truncated 5' long terminal repeat (LTR) from HIV-1  color: #ffe4c4"
     misc_feature    410..563
                     /vntifkey="21"
                     /label=SL123
                     /note="SL123 color: #a6acb3"
     misc_feature    455..580
                     /vntifkey="21"
                     /label=HIV-1\Psi
                     /note="HIV-1 Psi packaging signal of human immunodeficiency virus  type 1 color: #a6acb3"
     CDS             564..927
                     /codon_start=1
                     /vntifkey="4"
                     /label=SL4\(Gag)
                     /note="SL4 Psi Stem-Loop 4 (SL4) coded in Gag protein  color: #993366"
     misc_feature    1073..1306
                     /vntifkey="21"
                     /label=RRE
                     /note="RRE The Rev response element (RRE) of HIV-1 allows for  Rev-dependent mRNA export from the nucleus to the cytoplasm. color: #a6acb3"
     misc_feature    1802..1919
                     /vntifkey="21"
                     /label=cPPT/CTS
                     /note="cPPT/CTS central polypurine tract and central termination  sequence of HIV-1 color: #a6acb3"
     promoter        1944..3122
                     /vntifkey="30"
                     /label=EF-1a
                     /note="EF-1a color: #ffffff"
     misc_feature    4618..4685
                     /vntifkey="21"
                     /label=Spacer\for\EMCV\IRES
                     /note="Spacer for EMCV IRES From 11 pRLLSIN.CMV.cLRGAG.IRES.Zeo.WPRE.v2  color: #a6acb3"
     misc_feature    4686..5246
                     /vntifkey="21"
                     /label=EMCV\IRES
                     /note="EMCV IRES color: #a6acb3"
     misc_feature    6354..6942
                     /vntifkey="21"
                     /label=WPRE
                     /note="WPRE woodchuck hepatitis virus posttranscriptional  regulatory element color: #a6acb3"
     CDS             complement(6825..6836)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /vntifkey="4"
                     /label=Factor\Xa\site
                     /note="Factor Xa site color: #993366"
     LTR             7029..7262
                     /vntifkey="19"
                     /label=3'\LTR\(Delta-U3RU5)
                     /note="3' LTR self-inactivating 3' long terminal repeat (LTR) from HIV-1 color: #ffe4c4"
     polyA_signal    7334..7455
                     /vntifkey="25"
                     /label=SV40\poly(A)\signal
                     /note="SV40 poly SV40 polyadenylation signal  color: #a6acb3"
     rep_origin      7495..7630
                     /vntifkey="33"
                     /label=SV40\ori
                     /note="SV40 ori SV40 origin of replication  color: #ffff00"
     promoter        complement(7651..7669)
                     /vntifkey="30"
                     /label=T7\promoter
                     /note="T7 promoter promoter for bacteriophage T7 RNA polymerase  color: #ffffff; direction: LEFT"
     rep_origin      7837..8292
                     /direction=RIGHT
                     /vntifkey="33"
                     /label=f1\ori
                     /note="f1 ori f1 bacteriophage origin of replication; arrow  indicates direction of (+) strand synthesis color: #ffff00"
     promoter        8318..8422
                     /gene="bla"
                     /vntifkey="30"
                     /label=AmpR\promoter
                     /note="AmpR promoter color: #ffffff"
     CDS             8423..9283
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /vntifkey="4"
                     /label=AmpR
                     /note="AmpR confers resistance to ampicillin, carbenicillin, and related antibiotics color: #993366"
     rep_origin      9454..10042
                     /direction=RIGHT
                     /vntifkey="33"
                     /label=ori
                     /note="ori high-copy-number ColE1/pMB1/pBR322/pUC origin of  replication color: #ffff00"
     protein_bind    10330..10351
                     /bound_moiety="E. coli catabolite activator protein"
                     /vntifkey="31"
                     /label=CAP\binding\site
                     /note="CAP binding site CAP binding activates transcription in the presence  of cAMP. color: #31849b"
     promoter        10366..10396
                     /vntifkey="30"
                     /label=lac\promoter
                     /note="lac promoter promoter for the E. coli lac operon  color: #ffffff"
     protein_bind    10404..10420
                     /bound_moiety="lac repressor encoded by lacI"
                     /vntifkey="31"
                     /label=lac\operator
                     /note="lac operator The lac repressor binds to the lac operator to  inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG). color: #31849b"
     promoter        10465..1
                     /vntifkey="30"
                     /label=Promoter_P_1
                     /note="color: #ffffff"
     promoter        10465..10483
                     /vntifkey="30"
                     /label=T3\promoter
                     /note="T3 promoter promoter for bacteriophage T3 RNA polymerase  color: #ffffff"
     misc_feature    5253..6347
                     /vntifkey="21"
                     /label=GFP-ZEO
     misc_feature    3931..3939
                     /vntifkey="21"
                     /label=AAA\linker\(also\NotI)
     misc_feature    3940..4146
                     /vntifkey="21"
                     /label=CD8A
     misc_feature    4075..4146
                     /vntifkey="21"
                     /label=TM\region
     misc_feature    4147..4272
                     /vntifkey="21"
                     /label=41BB
     misc_feature    4273..4611
                     /vntifkey="21"
                     /label=CD3Z
     misc_feature    3151..3216
                     /vntifkey="21"
                     /label=GMCSF\Leader\Signal
     misc_feature    3217..3564
                     /vntifkey="21"
                     /label=J1.B5\VH
     misc_feature    3565..3609
                     /vntifkey="21"
                     /label=(GGGGS)3\linker
     misc_feature    3610..3930
                     /vntifkey="21"
                     /label=J1.B5\VL
BASE COUNT     2522 a      2701 c      2877 g      2410 t 
ORIGIN
        1 tgtagtctta tgcaatactc ttgtagtctt gcaacatggt aacgatgagt tagcaacatg 
       61 ccttacaagg agagaaaaag caccgtgcat gccgattggt ggaagtaagg tggtacgatc 
      121 gtgccttatt aggaaggcaa cagacgggtc tgacatggat tggacgaacc actgaattgc 
      181 cgcattgcag agatattgta tttaagtgcc tagctcgata cataaacggg tctctctggt 
      241 tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc 
      301 aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta 
      361 actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt ggcgcccgaa 
      421 cagggacttg aaagcgaaag ggaaaccaga ggagctctct cgacgcagga ctcggcttgc 
      481 tgaagcgcgc acggcaagag gcgaggggcg gcgactggtg agtacgccaa aaattttgac 
      541 tagcggaggc tagaaggaga gagatgggtg cgagagcgtc agtattaagc gggggagaat 
      601 tagatcgcga tgggaaaaaa ttcggttaag gccaggggga aagaaaaaat ataaattaaa 
      661 acatatagta tgggcaagca gggagctaga acgattcgca gttaatcctg gcctgttaga 
      721 aacatcagaa ggctgtagac aaatactggg acagctacaa ccatcccttc agacaggatc 
      781 agaagaactt agatcattat ataatacagt agcaaccctc tattgtgtgc atcaaaggat 
      841 agagataaaa gacaccaagg aagctttaga caagatagag gaagagcaaa acaaaagtaa 
      901 gaccaccgca cagcaagcgg cctctgatct tcagacctgg aggaggagat atgagggaca 
      961 attggagaag tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac 
     1021 ccaccaaggc aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt 
     1081 tgttccttgg gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga 
     1141 cggtacaggc cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg 
     1201 ctattgaggc gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg 
     1261 caagaatcct ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt 
     1321 gctctggaaa actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat 
     1381 ctctggaaca gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt 
     1441 acacaagctt aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac 
     1501 aagaattatt ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt 
     1561 ggctgtggta tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag 
     1621 tttttgctgt actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc 
     1681 agacccacct cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg 
     1741 gagagagaga cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcggttaa 
     1801 cttttaaaag aaaagggggg attggggggt acagtgcagg ggaaagaata gtagacataa 
     1861 tagcaacaga catacaaact aaagaattac aaaaacaaat tacaaaaatt caaaatttta 
     1921 tcgatcacga gactagcctc gagggctccg gtgcccgtca gtgggcagag cgcacatcgc 
     1981 ccacagtccc cgagaagttg gggggagggg tcggcaattg aaccggtgcc tagagaaggt 
     2041 ggcgcggggt aaactgggaa agtgatgtcg tgtactggct ccgccttttt cccgagggtg 
     2101 ggggagaacc gtatataagt gcagtagtcg ccgtgaacgt tctttttcgc aacgggtttg 
     2161 ccgccagaac acaggtaagt gccgtgtgtg gttcccgcgg gcctggcctc tttacgggtt 
     2221 atggcccttg cgtgccttga attacttcca cctggctgca gtacgtgatt cttgatcccg 
     2281 agcttcgggt tggaagtggg tgggagagtt cgaggccttg cgcttaagga gccccttcgc 
     2341 ctcgtgcttg agttgaggcc tggcctgggc gctggggccg ccgcgtgcga atctggtggc 
     2401 accttcgcgc ctgtctcgct gctttcgata agtctctagc catttaaaat ttttgatgac 
     2461 ctgctgcgac gctttttttc tggcaagata gtcttgtaaa tgcgggccaa gatctgcaca 
     2521 ctggtatttc ggtttttggg gccgcgggcg gcgacggggc ccgtgcgtcc cagcgcacat 
     2581 gttcggcgag gcggggcctg cgagcgcggc caccgagaat cggacggggg tagtctcaag 
     2641 ctggccggcc tgctctggtg cctggcctcg cgccgccgtg tatcgccccg ccctgggcgg 
     2701 caaggctggc ccggtcggca ccagttgcgt gagcggaaag atggccgctt cccggccctg 
     2761 ctgcagggag ctcaaaatgg aggacgcggc gctcgggaga gcgggcgggt gagtcaccca 
     2821 cacaaaggaa aagggccttt ccgtcctcag ccgtcgcttc atgtgactcc acggagtacc 
     2881 gggcgccgtc caggcacctc gattagttct cgagcttttg gagtacgtcg tctttaggtt 
     2941 ggggggaggg gttttatgcg atggagtttc cccacactga gtgggtggag actgaagtta 
     3001 ggccagcttg gcacttgatg taattctcct tggaatttgc cctttttgag tttggatctt 
     3061 ggttcattct caagcctcag acagtggttc aaagtttttt tcttccattt caggtgtcgt 
     3121 gaggaattag cttggtacga attcgccacc atgcttctcc tggtgacaag ccttctgctc 
     3181 tgtgagttac cacacccagc attcctcctg atcccagagg tgcagctgct ggagtccgga 
     3241 ggaggactgg tgcagccagg aggatccctg aggctgtctt gcgccgccag cggcttcacc 
     3301 tttagctcct acgcaatgtc ttgggtgcgc caggcacctg gcaagggact ggagtgggtg 
     3361 agcacaatcg caggatccgg aaccgcaaca gactacgccg attctgtgaa gggccggttc 
     3421 accatctcca gagacaactc taagaataca ctgtatctgc agatgaacag cctgcgggcc 
     3481 gaggataccg ccgtgtacta ttgtgccaag gacacatacg cctttgatta ttggggccag 
     3541 ggcaccctgg tgacagtgtc tagcggcggc ggaggcagcg gcggcggagg cagcggcggc 
     3601 ggaggcagcg acatccagat gacccagagc cctagctccc tgagcgcctc cgtgggcgat 
     3661 agggtgacca tcacatgcag agcctctcag agcatctcta gctacctgaa ctggtatcag 
     3721 cagaagcccg gcaaggcccc taagctgctg atctacgcag caagctatct gcagtccgga 
     3781 gtgccatctc ggttctccgg atctggaagc ggaaccgact ttaccctgac aatctcctct 
     3841 ctgcagccag aggatttcgc cacatactat tgtcagcagg cctactccgc cccctctacc 
     3901 tttggccagg gcacaaaggt ggagatcaag gcggccgcaa ccacgacgcc agcgccgcga 
     3961 ccaccaacac cggcgcccac catcgcgtcg cagcccctgt ccctgcgccc agaggcgtgc 
     4021 cggccagcgg cggggggcgc agtgcacacg agggggctgg acttcgcctg tgatatctac 
     4081 atctgggcgc ccttggccgg gacttgtggg gtccttctcc tgtcactggt tatcaccctt 
     4141 tactgcaaac ggggcagaaa gaaactcctg tatatattca aacaaccatt tatgagacca 
     4201 gtacaaacta ctcaagagga agatggctgt agctgccgat ttccagaaga agaagaagga 
     4261 ggatgtgaac tgagagtgaa gttcagcagg agcgcagacg cccccgcgta ccagcagggc 
     4321 cagaaccagc tctataacga gctcaatcta ggacgaagag aggagtacga tgttttggac 
     4381 aagagacgtg gccgggaccc tgagatgggg ggaaagccga gaaggaagaa ccctcaggaa 
     4441 ggcctgtaca atgaactgca gaaagataag atggcggagg cctacagtga gattgggatg 
     4501 aaaggcgagc gccggagggg caaggggcac gatggccttt accagggtct cagtacagcc 
     4561 accaaggaca cctacgacgc ccttcacatg caggccctgc cccctcgcta acgtacgccc 
     4621 gcgaaattaa tacgactcac tatagggaga ccacaacggt ttccctctag cgggatcaat 
     4681 tccgcccccc ccctaacgtt actggccgaa gccgcttgga ataaggccgg tgtgcgtttg 
     4741 tctatatgtt attttccacc atattgccgt cttttggcaa tgtgagggcc cggaaacctg 
     4801 gccctgtctt cttgacgagc attcctaggg gtctttcccc tctcgccaaa ggaatgcaag 
     4861 gtctgttgaa tgtcgtgaag gaagcagttc ctctggaagc ttcttgaaga caaacaacgt 
     4921 ctgtagcgac cctttgcagg cagcggaacc ccccacctgg cgacaggtgc ctctgcggcc 
     4981 aaaagccacg tgtataagat acacctgcaa aggcggcaca accccagtgc cacgttgtga 
     5041 gttggatagt tgtggaaaga gtcaaatggc tctcctcaag cgtattcaac aaggggctga 
     5101 aggatgccca gaaggtaccc cattgtatgg gatctgatct ggggcctcgg tgcacatgct 
     5161 ttacatgtgt ttagtcgagg ttaaaaaaac gtctaggccc cccgaaccac ggggacgtgg 
     5221 ttttcctttg aaaacacgat gataatacta gtatggtgag caagggcgag gagctgttca 
     5281 ccggggtggt gcccatcctg gtcgagctgg acggcgacgt aaacggccac aagttcagcg 
     5341 tgtccggcga gggcgagggc gatgccacct acggcaagct gaccctgaag ttcatctgca 
     5401 ccaccggcaa gctgcccgtg ccctggccca ccctcgtgac caccctgacc tacggcgtgc 
     5461 agtgcttcag ccgctacccc gaccacatga agcagcacga cttcttcaag tccgccatgc 
     5521 ccgaaggcta cgtccaggag cgcaccatct tcttcaagga cgacggcaac tacaagaccc 
     5581 gcgccgaggt gaagttcgag ggcgacaccc tggtgaaccg catcgagctg aagggcatcg 
     5641 acttcaagga ggacggcaac atcctggggc acaagctgga gtacaactac aacagccaca 
     5701 acgtctatat catggccgac aagcagaaga acggcatcaa ggtgaacttc aagatccgcc 
     5761 acaacatcga ggacggcagc gtgcagctcg ccgaccacta ccagcagaac acccccatcg 
     5821 gcgacggccc cgtgctgctg cccgacaacc actacctgag cacccagtcc gccctgagca 
     5881 aagaccccaa cgagaagcgc gatcacatgg tcctgctgga gttcgtgacc gccgccggga 
     5941 tcactctcgg catggacgag ctgtacaagg gcatggccaa gttgaccagt gccgttccgg 
     6001 tgctcaccgc gcgcgacgtc gccggagcgg tcgagttctg gaccgaccgg ctcgggttct 
     6061 cccgggactt cgtggaggac gacttcgccg gtgtggtccg ggacgacgtg accctgttca 
     6121 tcagcgcggt ccaggaccag gtggtgccgg acaacaccct ggcctgggtg tgggtgcgcg 
     6181 gcctggacga gctgtacgcc gagtggtcgg aggtcgtgtc cacgaacttc cgggacgcct 
     6241 ccgggccggc catgaccgag atcggcgagc agccgtgggg gcgggagttc gccctgcgcg 
     6301 acccggccgg caactgcgtg cacttcgtgg ccgaggagca ggactgagtc gacaatcaac 
     6361 ctctggatta caaaatttgt gaaagattga ctggtattct taactatgtt gctcctttta 
     6421 cgctatgtgg atacgctgct ttaatgcctt tgtatcatgc tattgcttcc cgtatggctt 
     6481 tcattttctc ctccttgtat aaatcctggt tgctgtctct ttatgaggag ttgtggcccg 
     6541 ttgtcaggca acgtggcgtg gtgtgcactg tgtttgctga cgcaaccccc actggttggg 
     6601 gcattgccac cacctgtcag ctcctttccg ggactttcgc tttccccctc cctattgcca 
     6661 cggcggaact catcgccgcc tgccttgccc gctgctggac aggggctcgg ctgttgggca 
     6721 ctgacaattc cgtggtgttg tcggggaagc tgacgtcctt tccatggctg ctcgcctgtg 
     6781 ttgccacctg gattctgcgc gggacgtcct tctgctacgt cccttcggcc ctcaatccag 
     6841 cggaccttcc ttcccgcggc ctgctgccgg ctctgcggcc tcttccgcgt cttcgccttc 
     6901 gccctcagac gagtcggatc tccctttggg ccgcctcccc gcctgtaatt cgagctcggt 
     6961 acctttaaga ccaatgactt acaaggcagc tgtagatctt agccactttt taaaagaaaa 
     7021 ggggggactg gaagggctaa ttcactccca acgaagacaa gatctgcttt ttgcttgtac 
     7081 tgggtctctc tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc 
     7141 actgcttaag cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt 
     7201 gtgtgactct ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag 
     7261 cagtagtagt tcatgtcatc ttattattca gtatttataa cttgcaaaga aatgaatatc 
     7321 agagagtgag aggaacttgt ttattgcagc ttataatggt tacaaataaa gcaatagcat 
     7381 cacaaatttc acaaataaag catttttttc actgcattct agttgtggtt tgtccaaact 
     7441 catcaatgta tcttatcatg tctggctcta gctatcccgc ccctaactcc gcccatcccg 
     7501 cccctaactc cgcccagttc cgcccattct ccgccccatg gctgactaat tttttttatt 
     7561 tatgcagagg ccgaggccgc ctcggcctct gagctattcc agaagtagtg aggaggcttt 
     7621 tttggaggcc tagggacgta cccaattcgc cctatagtga gtcgtattac gcgcgctcac 
     7681 tggccgtcgt tttacaacgt cgtgactggg aaaaccctgg cgttacccaa cttaatcgcc 
     7741 ttgcagcaca tccccctttc gccagctggc gtaatagcga agaggcccgc accgatcgcc 
     7801 cttcccaaca gttgcgcagc ctgaatggcg aatgggacgc gccctgtagc ggcgcattaa 
     7861 gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac acttgccagc gccctagcgc 
     7921 ccgctccttt cgctttcttc ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag 
     7981 ctctaaatcg ggggctccct ttagggttcc gatttagtgc tttacggcac ctcgacccca 
     8041 aaaaacttga ttagggtgat ggttcacgta gtgggccatc gccctgatag acggtttttc 
     8101 gccctttgac gttggagtcc acgttcttta atagtggact cttgttccaa actggaacaa 
     8161 cactcaaccc tatctcggtc tattcttttg atttataagg gattttgccg atttcggcct 
     8221 attggttaaa aaatgagctg atttaacaaa aatttaacgc gaattttaac aaaatattaa 
     8281 cgcttacaat ttaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt 
     8341 tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca 
     8401 ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt 
     8461 ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga 
     8521 tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa 
     8581 gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct 
     8641 gctatgtggc gcggtattat cccgtattga cgccgggcaa gagcaactcg gtcgccgcat 
     8701 acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga 
     8761 tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc 
     8821 caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat 
     8881 gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa 
     8941 cgacgagcgt gacaccacga tgcctgtagc aatggcaaca acgttgcgca aactattaac 
     9001 tggcgaacta cttactctag cttcccggca acaattaata gactggatgg aggcggataa 
     9061 agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc 
     9121 tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc 
     9181 ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag 
     9241 acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag accaagttta 
     9301 ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa 
     9361 gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc 
     9421 gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat 
     9481 ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga 
     9541 gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt 
     9601 tcttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata 
     9661 cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac 
     9721 cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg 
     9781 ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg 
     9841 tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag 
     9901 cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct 
     9961 ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc 
    10021 aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt 
    10081 ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg 
    10141 tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga 
    10201 gtcagtgagc gaggaagcgg aagagcgccc aatacgcaaa ccgcctctcc ccgcgcgttg 
    10261 gccgattcat taatgcagct ggcacgacag gtttcccgac tggaaagcgg gcagtgagcg 
    10321 caacgcaatt aatgtgagtt agctcactca ttaggcaccc caggctttac actttatgct 
    10381 tccggctcgt atgttgtgtg gaattgtgag cggataacaa tttcacacag gaaacagcta 
    10441 tgaccatgat tacgccaagc gcgcaattaa ccctcactaa agggaacaaa agctggagct 
    10501 gcaagcttaa 
//
