LOCUS       pHERD30T_crRNA_L        5202 bp    DNA        circular     04-SEP-2020
DEFINITION  .
KEYWORDS    "accession:EU603326"                                               
COMMENT     
COMMENT     ApEinfo:methylated:1
FEATURES             Location/Qualifiers
     rep_origin      complement(56..675)
                     /locus_tag="rep_origin"
                     /label="rep_origin"
                     /ApEinfo_label="rep_origin"
                     /ApEinfo_fwdcolor="#85dae9"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    2231..2240
                     /locus_tag="Unimportant Filler"
                     /label="Unimportant Filler"
                     /ApEinfo_label="Unimportant Filler"
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     rep_origin      complement(3318..3718)
                     /locus_tag="rep_origin(1)"
                     /label="rep_origin(1)"
                     /ApEinfo_label="rep_origin"
                     /ApEinfo_fwdcolor="#84b0dc"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     protein_bind    2225..2230
                     /locus_tag="BsaI Cut Site"
                     /label="BsaI Cut Site"
                     /ApEinfo_label="BsaI Cut Site"
                     /ApEinfo_fwdcolor="#ffd800"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     CDS             complement(2484..3317)
                     /locus_tag="RepB"
                     /label="RepB"
                     /ApEinfo_label="RepB"
                     /ApEinfo_fwdcolor="#faac61"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     protein_bind    2241..2246
                     /locus_tag="BsaI Cut Site(1)"
                     /label="BsaI Cut Site(1)"
                     /ApEinfo_label="BsaI Cut Site"
                     /ApEinfo_fwdcolor="#ffe500"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     CDS             3940..4473
                     /locus_tag="Gentamicin R"
                     /label="Gentamicin R"
                     /ApEinfo_label="Gentamicin R"
                     /ApEinfo_fwdcolor="#ff9ccd"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     repeat_unit     2187..2224
                     /locus_tag="Lse Direct Repeat"
                     /label="Lse Direct Repeat"
                     /ApEinfo_label="Lse Direct Repeat"
                     /ApEinfo_fwdcolor="#ff6666"
                     /ApEinfo_revcolor="#ff6666"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     oriT            complement(725..993)
                     /locus_tag="oriT"
                     /label="oriT"
                     /ApEinfo_label="oriT"
                     /ApEinfo_fwdcolor="#f58a5e"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     CDS             complement(997..1875)
                     /locus_tag="AraC"
                     /label="AraC"
                     /ApEinfo_label="AraC"
                     /ApEinfo_fwdcolor="#c6c9d1"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    2109..2128
                     /locus_tag="misc_feature"
                     /label="misc_feature"
                     /ApEinfo_label="misc_feature"
                     /ApEinfo_fwdcolor="#b7e6d7"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     -35_signal      2149..2154
                     /locus_tag="-35"
                     /label="-35"
                     /ApEinfo_label="-35"
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    2173..2178
                     /locus_tag="misc_feature(1)"
                     /label="misc_feature(1)"
                     /ApEinfo_label="misc_feature"
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     CDS             2248..2487
                     /locus_tag="LacZ alpha"
                     /label="LacZ alpha"
                     /ApEinfo_label="LacZ alpha"
                     /ApEinfo_fwdcolor="#84b0dc"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     primer_bind     2164..2186
                     /locus_tag="crRNA (VI) Seq F"
                     /label="crRNA (VI) Seq F"
                     /ApEinfo_label="crRNA (VI) Seq F"
                     /ApEinfo_fwdcolor="#14c0bd"
                     /ApEinfo_revcolor="#4ec02b"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     primer_bind     2256..2275
                     /locus_tag="crRNA (VI) Seq R"
                     /label="crRNA (VI) Seq R"
                     /ApEinfo_label="crRNA (VI) Seq R"
                     /ApEinfo_fwdcolor="#14c0bd"
                     /ApEinfo_revcolor="#4ec02b"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    2187..2187
                     /locus_tag="TSS"
                     /label="TSS"
                     /ApEinfo_label="TSS"
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     -10_signal      2173..2178
                     /locus_tag="-10"
                     /label="-10"
                     /ApEinfo_label="-10"
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
ORIGIN
        1 accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc
       61 aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa
      121 ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag
      181 gtaactggct tcagcagagc gcagatacca aatactgtcc ttctagtgta gccgtagtta
      241 ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta
      301 ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag
      361 ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg
      421 gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg
      481 cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag
      541 cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc
      601 cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa
      661 aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacata
      721 agctagctta tcggccagcc tcgcagagca ggattcccgt tgagcaccgc caggtgcgaa
      781 taagggacag tgaagaagga acacccgctc gcgggtgggc ctacttcacc tatcctgccc
      841 ggctgacgcc gttggataca ccaaggaaag tctacacgaa ccctttggca aaatcctgta
      901 tatcgtgcga aaaaggatgg atataccgaa aaaatcgcta taatgacccc gaagcagggt
      961 tatgcagcgg aaagtatacc ttaaggaatc cccaaattat gacaacttga cggctacatc
     1021 attcactttt tcttcacaac cggcacggaa ctcgctcggg ctggccccgg tgcatttttt
     1081 aaatacccgc gagaaataga gttgatcgtc aaaaccaaca ttgcgaccga cggtggcgat
     1141 aggcatccgg gtggtgctca aaagcagctt cgcctggctg atacgttggt cctcgcgcca
     1201 gcttaagacg ctaatcccta actgctggcg gaaaagatgt gacagacgcg acggcgacaa
     1261 gcaaacatgc tgtgcgacgc tggcgatatc aaaattgctg tctgccaggt gatcgctgat
     1321 gtactgacaa gcctcgcgta cccgattatc catcggtgga tggagcgact cgttaatcgc
     1381 ttccatgcgc cgcagtaaca attgctcaag cagatttatc gccagcagct ccgaatagcg
     1441 cccttcccct tgcccggcgt taatgatttg cccaaacagg tcgctgaaat gcggctggtg
     1501 cgcttcatcc gggcgaaaga accccgtatt ggcaaatatt gacggccagt taagccattc
     1561 atgccagtag gcgcgcggac gaaagtaaac ccactggtga taccattcgc gagcctccgg
     1621 atgacgaccg tagtgatgaa tctctcctgg cgggaacagc aaaatatcac ccggtcggca
     1681 aacaaattct cgtccctgat ttttcaccac cccctgaccg cgaatggtga gattgagaat
     1741 ataacctttc attcccagcg gtcggtcgat aaaaaaatcg agataaccgt tggcctcaat
     1801 cggcgttaaa cccgccacca gatgggcatt aaacgagtat cccggcagca ggggatcatt
     1861 ttgcgcttca gccatacttt tcatactccc gccattcaga gaagaaacca attgtccata
     1921 ttgcatcaga cattgccgtc actgcgtctt ttactggctc ttctcgctaa ccaaaccggt
     1981 aaccccgctt attaaaagca ttctgtaaca aagcgggacc aaagccatga caaaaacgcg
     2041 taacaaaagt gtctataatc acggcagaaa agtccacatt gattatttgc acggcgtcac
     2101 actttgctat gccatagcat ttttatccat aagattagcg gatcctacct gacgcttttt
     2161 atcgcaactc tctactgttt ctccatGGTA AGAGACTACC TCTATATGAA AGAGGACTAA
     2221 AACCgagacc ACGTACGTAC ggtctcgaat tcgagctcgg tacccgggga tcctctagag
     2281 tcgacctgca ggcatgcaag cttggcactg gccgtcgttt tacaacgtcg tgactgggaa
     2341 aaccctggcg ttacccaact taatcgcctt gcagcacatc cccctttcgc cagctggcgt
     2401 aatagcgaag aggcccgcac cgatcgccct tcccaacagt tgcgcagcct gaaaggcagg
     2461 ccgggccgtg gtggccacgg cctctaggcc agatccagcg gcatctgggt tagtcgagcg
     2521 cgggccgctt cccatgtctc accagggcga gcctgtttcg cgatctcagc atctgaaatc
     2581 ttcccggcct tgcgcttcgc tggggcctta cccaccgcct tggcgggctt cttcggtcca
     2641 aaactgaaca acagatgtgt gaccttgcgc ccggtctttc gctgcgccca ctccacctgt
     2701 agcgggctgt gctcgttgat ctgcgtcacg gctggatcaa gcactcgcaa cttgaagtcc
     2761 ttgatcgagg gataccggcc ttccagttga aaccactttc gcagctggtc aatttctatt
     2821 tcgcgctggc cgatgctgtc ccattgcatg agcagctcgt aaagcctgat cgcgtgggtg
     2881 ctgtccatct tggccacgtc agccaaggcg tatttggtga actgtttggt gagttccgtc
     2941 aggtacggca gcatgtcttt ggtgaacctg agttctacac ggccctcacc ctcccggtag
     3001 atgattgttt gcacccagcc ggtaatcatc acactcggtc ttttcccctt gccattgggc
     3061 tcttgggtta accggacttc ccgccgtttc aggcgcaggg ccgcttcttt gagctggttg
     3121 taggaagatt cgatagggac acccgccatc gtcgctatgt cctccgccgt cactgaatac
     3181 atcacttcat cggtgacagg ctcgctcctc ttcacctggc taatacaggc cagaacgatc
     3241 cgctgttcct gaacactgag gcgatacgcg gcctcgacca gggcattgct tttgtaaacc
     3301 attgggggtg aggccacgtt cgacattcct tgtgtataag gggacactgt atctgcgtcc
     3361 cacaatacaa caaatccgtc cctttacaac aacaaatccg tcccttctta acaacaaatc
     3421 cgtcccttaa tggcaacaaa tccgtccctt tttaaactct acaggccacg gattacgtgg
     3481 cctgtagacg tcctaaaagg tttaaaaggg aaaaggaaga aaagggtgga aacgcaaaaa
     3541 acgcaccact acgtggcccc gttggggccg catttgtgcc cctgaagggg cgggggaggc
     3601 gtctgggcaa tccccgtttt accagtcccc tatcgccgcc tgagagggcg caggaagcga
     3661 gtaatcaggg tatcgaggcg gattcaccct tggcgtccaa ccagcggcac cagcggcgcg
     3721 aattgacata agcctgttcg gttcgtaaac tgtaatgcaa gtagcgtatg cgctcacgca
     3781 actggtccag aaccttgacc gaacgcagcg gtggtaacgg cgcagtggcg gttttcatgg
     3841 cttgttatga ctgttttttt gtacagtcta tgcctcgggc atccaagcag caagcgcgtt
     3901 acgccgtggg tcgatgtttg atgttatgga gcagcaacga tgttacgcag cagcaacgat
     3961 gttacgcagc agggcagtcg ccctaaaaca aagttaggtg gctcaagtat gggcatcatt
     4021 cgcacatgta ggctcggccc tgaccaagtc aaatccatgc gggctgctct tgatcttttc
     4081 ggtcgtgagt tcggagacgt agccacctac tcccaacatc agccggactc cgattacctc
     4141 gggaacttgc tccgtagtaa gacattcatc gcgcttgctg ccttcgacca agaagcggtt
     4201 gttggcgctc tcgcggctta cgttctgccc aggtttgagc agccgcgtag tgagatctat
     4261 atctatgatc tcgcagtctc cggcgagcac cggaggcagg gcattgccac cgcgctcatc
     4321 aatctcctca agcatgaggc caacgcgctt ggtgcttatg tgatctacgt gcaagcagat
     4381 tacggtgacg atcccgcagt ggctctctat acaaagttgg gcatacggga agaagtgatg
     4441 cactttgata tcgacccaag taccgccacc taacaattcg ttcaagccga gatcggcttc
     4501 ccggccgcgg agttgttcgg taaattgtca caacgccgcg gccaattcga ctcaccagtc
     4561 acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc
     4621 atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta
     4681 accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag
     4741 ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgtagc aatggcaaca
     4801 acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca acaattaata
     4861 gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc
     4921 tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca
     4981 ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca
     5041 actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg
     5101 taactgtcag accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa
     5161 tttaaaagga tctaggtgaa gatccttttt gataatctca tg 
//
