LOCUS       pAS154_(synAR_Part_(Par 3757 bp ds-DNA     circular     15-NOV-2022
DEFINITION  .
KEYWORDS    "Selection:Cam"
FEATURES             Location/Qualifiers
     RBS             20..25
                     /label="Kozak"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     CDS             26..280
                     /label="ZF 92-1"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
     CDS             281..304
                     /label="linker1"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     CDS             311..1177
                     /label="AR LBD"
                     /ApEinfo_revcolor="#0080ff"
                     /ApEinfo_fwdcolor="#0080ff"
     misc_feature    791..791
                     /label="T->C"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     CDS             1178..1201
                     /label="linker2"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     CDS             1208..2095
                     /label="Msn2 activation domain"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     misc_feature    complement(2118..2226)
                     /label="CamR Terminator"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     CDS             complement(2227..2886)
                     /label="CamR"
                     /ApEinfo_revcolor="#0000ff"
                     /ApEinfo_fwdcolor="#0000ff"
     promoter        complement(2887..2991)
                     /label="CamR Promoter"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     rep_origin      complement(2992..3755)
                     /label="ColE1"
                     /ApEinfo_revcolor="#7f7f7f"
                     /ApEinfo_fwdcolor="#7f7f7f"
ORIGIN
        1 TCGGTCTCAT ATGCTCGAGA AAAAAatgga gcgccccttc cagtgtcgca tttgcatgcg
       61 gaacttttcg gattctccaa ctttgagaag acatacccgt actcataccg gtgaaaaacc
      121 gtttcagtgt cggatctgta tgcgaaattt ctcccaaaga tcatctttgg ttagacatct
      181 acgtacgcac accggcgaga agccattcca atgccgaata tgcatgcgca acttcagtga
      241 aagaggtaat ttgactagac acctaaaaac ccacctgaga ggagatggtg ctggattgat
      301 tgatATCGAT ctgaagaaac ttggtaatct gaaactacag gaggaaggag aggcttccag
      361 caccaccagc cccactgagg agacaaccca gaagctgaca gtgtcacaca ttgaaggcta
      421 tgaatgtcag cccatctttc tgaatgtcct ggaagccatt gagccaggtg tagtgtgtgc
      481 tggacacgac aacaaccagc ccgactcctt tgcagccttg ctctctagcc tcaatgaact
      541 gggagagaga cagcttgtac acgtggtcaa gtgggccaag gccttgcctg gcttccgcaa
      601 cttacacgtg gacgaccaga tggctgtcat tcagtactcc tggatggggc tcatggtgtt
      661 tgccatgggc tggcgatcct tcaccaatgt caactccagg atgctctact tcgcccctga
      721 tctggttttc aatgagtacc gcatgcacaa gtcccggatg tacagccagt gtgtccgaat
      781 gaggcacctc Cctcaagagt ttggatggct ccaaatcacc ccccaggaat tcctgtgcat
      841 gaaagcactg ctactcttca gcattattcc agtggatggg ctgaaaaatc aaaaattctt
      901 tgatgaactt cgaatgaact acatcaagga actcgatcgt atcattgcat gcaaaagaaa
      961 aaatcccaca tcctgctcaa gacgcttcta ccagctcacc aagctcctgg actccgtgca
     1021 gcctattgcg agagagctgc atcagttcac ttttgacctg ctaatcaagt cacacatggt
     1081 gagcgtggac tttccggaaa tgatggcaga gatcatctct gtgcaagtgc ccaagatcct
     1141 ttctgggaaa gtcaagccca tctatttcca cacccagggc gacggagcag gcttaataaa
     1201 tGCTAGCatg acggtcgacc atgatttcaa tagcgaagat attttattcc ccatagaaag
     1261 catgagtagt atacaatacg tggagaataa taacccaaat aatattaaca acgatgttat
     1321 cccgtattct ctagatatca aaaacactgt cttagatagt gcggatctca atgacattca
     1381 aaatcaagaa acttcactga atttggggct tcctccacta tctttcgact ctccactgcc
     1441 cgtaacggaa acgataccat ccactaccga taacagcttg catttgaaag ctgatagcaa
     1501 caaaaatcgc gatgcaagaa ctattgaaaa tgatagtgaa attaagagta ctaataatgc
     1561 tagtggctct ggggcaaatc aatacacaac tcttacttca ccttatccta tgaacgacat
     1621 tttgtacaac atgaacaatc cgttacaatc accgtcacct tcatcggtac ctcaaaatcc
     1681 gactataaat cctcccataa atacagcaag taacgaaact aatttatcgc ctcaaacttc
     1741 aaatggtaat gaaactctta tatctccacg agcccaacaa catacgtcca ttaaagataa
     1801 tcgtctgtcc ttacctaatg gtgctaattc gaatcttttc attgacacta acccaaacaa
     1861 tttgaacgaa aaactaagaa atcaattgaa ctcagataca aattcatatt ctaactccat
     1921 ttctaattca aactccaatt ctacgggtaa tttaaattcc agttatttta attcactgaa
     1981 catagactcc atgctagatg attacgtttc tagtgatctc ttattgaatg atgatgatga
     2041 tgacactaat ttatcacgcc gaagatttag cgacgttata acaaaccaag agctcTAAGG
     2101 GCCCATCCTG AGACCagacc aataaaaaac gcccggcggc aaccgagcgt tctgaacaaa
     2161 tccagatgga gttctgaggt cattactgga tctatcaaca ggagtccaag cgagctcgat
     2221 atcaaattac gccccgccct gccactcatc gcagtactgt tgtaattcat taagcattct
     2281 gccgacatgg aagccatcac aaacggcatg atgaacctga atcgccagcg gcatcagcac
     2341 cttgtcgcct tgcgtataat atttgcccat ggtgaaaacg ggggcgaaga agttgtccat
     2401 attggccacg tttaaatcaa aactggtgaa actcacccag ggattggctg aaacgaaaaa
     2461 catattctca ataaaccctt tagggaaata ggccaggttt tcaccgtaac acgccacatc
     2521 ttgcgaatat atgtgtagaa actgccggaa atcgtcgtgg tattcactcc agagcgatga
     2581 aaacgtttca gtttgctcat ggaaaacggt gtaacaaggg tgaacactat cccatatcac
     2641 cagctcaccg tctttcattg ccatacgaaa ttccggatga gcattcatca ggcgggcaag
     2701 aatgtgaata aaggccggat aaaacttgtg cttatttttc tttacggtct ttaaaaaggc
     2761 cgtaatatcc agctgaacgg tctggttata ggtacattga gcaactgact gaaatgcctc
     2821 aaaatgttct ttacgatgcc attgggatat atcaacggtg gtatatccag tgattttttt
     2881 ctccatttta gcttccttag ctcctgaaaa tctcgataac tcaaaaaata cgcccggtag
     2941 tgatcttatt tcattatggt gaaagttgga acctcttacg tgcccgatca atcatgacca
     3001 aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag
     3061 gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac
     3121 cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa
     3181 ctggcttcag cagagcgcag ataccaaata ctgttcttct agtgtagccg tagttaggcc
     3241 accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag
     3301 tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac
     3361 cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc
     3421 gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc
     3481 ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca
     3541 cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc
     3601 tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg
     3661 ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct
     3721 ttcctgcgtt atcccctgat tctgtggata accgtag
//