LOCUS       pAS155_(synDHBR_Part_(P 3967 bp ds-DNA     circular     15-NOV-2022
DEFINITION  .
KEYWORDS    "Selection:Cam"
FEATURES             Location/Qualifiers
     RBS             20..25
                     /label="Kozak"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     CDS             26..280
                     /label="ZF 97-4"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
     CDS             281..304
                     /label="linker1"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     misc_feature    311..1705
                     /label="DHB"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     CDS             1706..1729
                     /label="linker2"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     CDS             1736..2305
                     /label="BRLF1 AD aa416-605 (Rta)"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     misc_feature    complement(2328..2436)
                     /label="CamR Terminator"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     CDS             complement(2437..3096)
                     /label="CamR"
                     /ApEinfo_revcolor="#0000ff"
                     /ApEinfo_fwdcolor="#0000ff"
     promoter        complement(3097..3201)
                     /label="CamR Promoter"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     rep_origin      complement(3202..3965)
                     /label="ColE1"
                     /ApEinfo_revcolor="#7f7f7f"
                     /ApEinfo_fwdcolor="#7f7f7f"
ORIGIN
        1 TCGGTCTCAT ATGCTCGAGA AAAAAatgga gcgacccttc cagtgcagga tttgcatgcg
       61 gaacttttcg agacaatcta atttgtcaag acatacaagg actcataccg gtgaaaaacc
      121 gtttcagtgt aggatctgta tgcgaaattt cagtagaaat gaacatttgg ttctacatct
      181 acgtacgcac acaggcgaaa agccattcca atgccgaata tgcatgcgca acttcagtca
      241 aaaaactggt cttagagttc atctaaaaac ccaccttaga ggagatggtg ctggattaat
      301 tgatATCGAT atgggtacaa gaccatatgc ttgtccagtt gagtcttgtg acagaagatt
      361 ctcaagaagc gacgaattaa cgcgtcacat tagaattcat acaggtcaaa aaccttttca
      421 gtgtagaatc tgcatgagaa acttttcacg ttcggatcac cttactactc atatcagaac
      481 gcacactggt gaaaaacctt ttgcatgtga tatttgtgga agaaaattcg caagatctga
      541 tgaaagaaag cgtcacacaa aaatccacac tggtgggggc ggaactccag ctgctgcttc
      601 caccttggaa gatcctagtg ctggggatat gcgtgctgct aacttgtggc cttctccatt
      661 aatgataaaa agatctaaaa agaattctct tgctttgtcg ttgactgcag atcaaatggt
      721 ttctgcttta ttagacgccg aacctcctat tttatactct gaatatgatc ctactaggcc
      781 attttcggaa gcctctatga tgggtttaat taccaatttg atggatagag aattagtgca
      841 tatgattaat tgggccaaga gggtccctgg ttttgttgat ttaaccttac acgatcaagt
      901 acatttatta gaatgtgctt ggcttgaaat cttgcaaatt ggtctagttt ggagatccat
      961 ggaacatcca gttaaattac tattcgctcc taatttgctt ttggacagaa accaaggtaa
     1021 gtgtgtagaa ggaatggttg aaatttttga tatgctactt gctacaagct caagattcag
     1081 aatgatgaac ttgcaaggtg aggaattcgt ttgtttgaaa tcgattatac tactcaattc
     1141 tggagtttac actttcttgt catccacgtt aaagtcttta gaagaaaagg accacataca
     1201 tagggtcctt gacaagatca ctgacacatt aattcattta atggccaagg ctggtttaac
     1261 cttgcaacaa caacaccaac gtttggccca attgttgttg atattgagtc acatacgtca
     1321 tatgtctaat aaatctatgg aacacctaga ctccatgaag tgtaagaacg tcgtacccct
     1381 atacgactta ttgttagaaa tgttggatgc acatagatta catgccccta ctagtagagg
     1441 tggagcatca gtagaggaaa ctgatcaatc gcatttggct actgcaggtt ccactagttc
     1501 cgaattacat ttagatggtg aggatgtagc tatggcccac gcagatgcgc tagatgattt
     1561 tgatctagac atgctcggtg acggggactc ccctggtccg gggtttaccc cacatgattc
     1621 cgccccatat ggcgctttgg atatggctga ctttgaattc gaacaaatgt ttactgatgc
     1681 attgggtatc gatgaatatg gtggtggcga cggagcaggc ttaataaatG CTAGCaggga
     1741 ttccagggaa gggatgtttt tgccgaaacc tgaagcgggc tcagcgatta gtgacgtgtt
     1801 tgagggccgc gaggtgtgcc agccaaaacg aataaggcca tttcatcctc caggaagtcc
     1861 atgggccaat cgcccactcc ccgcaagcct cgcaccaaca ccaaccggtc cagtacatga
     1921 gccagtcggg tcactgacac cggcaccagt ccctcagcca ctggatccag cgcccgcagt
     1981 gactcccgag gcgagtcacc tattggagga tcccgatgaa gaaacaagcc aggctgtcaa
     2041 agcccttcgg gagatggccg atacggtgat tcctcagaaa gaagaagcgg caatttgtgg
     2101 ccaaatggac ctttcacatc cgccccctag gggccatctg gatgaactga caaccacact
     2161 tgagtcaatg accgaggatc tgaacctgga ctcacccctg acgccggaac ttaatgagat
     2221 tctggataca ttcctgaacg acgagtgcct cttacatgca atgcatataa gcacaggact
     2281 atcaatcttt gacacatctc tgttttaaGG GCCCATCCTG AGACCagacc aataaaaaac
     2341 gcccggcggc aaccgagcgt tctgaacaaa tccagatgga gttctgaggt cattactgga
     2401 tctatcaaca ggagtccaag cgagctcgat atcaaattac gccccgccct gccactcatc
     2461 gcagtactgt tgtaattcat taagcattct gccgacatgg aagccatcac aaacggcatg
     2521 atgaacctga atcgccagcg gcatcagcac cttgtcgcct tgcgtataat atttgcccat
     2581 ggtgaaaacg ggggcgaaga agttgtccat attggccacg tttaaatcaa aactggtgaa
     2641 actcacccag ggattggctg aaacgaaaaa catattctca ataaaccctt tagggaaata
     2701 ggccaggttt tcaccgtaac acgccacatc ttgcgaatat atgtgtagaa actgccggaa
     2761 atcgtcgtgg tattcactcc agagcgatga aaacgtttca gtttgctcat ggaaaacggt
     2821 gtaacaaggg tgaacactat cccatatcac cagctcaccg tctttcattg ccatacgaaa
     2881 ttccggatga gcattcatca ggcgggcaag aatgtgaata aaggccggat aaaacttgtg
     2941 cttatttttc tttacggtct ttaaaaaggc cgtaatatcc agctgaacgg tctggttata
     3001 ggtacattga gcaactgact gaaatgcctc aaaatgttct ttacgatgcc attgggatat
     3061 atcaacggtg gtatatccag tgattttttt ctccatttta gcttccttag ctcctgaaaa
     3121 tctcgataac tcaaaaaata cgcccggtag tgatcttatt tcattatggt gaaagttgga
     3181 acctcttacg tgcccgatca atcatgacca aaatccctta acgtgagttt tcgttccact
     3241 gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg
     3301 taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc
     3361 aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata
     3421 ctgttcttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta
     3481 catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc
     3541 ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg
     3601 ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac
     3661 agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg
     3721 taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt
     3781 atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct
     3841 cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg
     3901 ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata
     3961 accgtag
//