LOCUS       pAS159_(synAR_Cassette) 5279 bp ds-DNA     circular     15-NOV-2022
DEFINITION  .
KEYWORDS    "Selection:Amp"
FEATURES             Location/Qualifiers
     misc_feature    16..158
                     /label="ConL1"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     misc_feature    166..190
                     /label="ConL1 scar"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     promoter        195..894
                     /label="ScTEF1 Promoter"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     RBS             910..915
                     /label="Kozak"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     CDS             916..1170
                     /label="ZF 92-1"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
     CDS             1171..1194
                     /label="linker1"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     CDS             1201..2067
                     /label="AR LBD"
                     /ApEinfo_revcolor="#0080ff"
                     /ApEinfo_fwdcolor="#0080ff"
     misc_feature    1681..1681
                     /label="T->C"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     CDS             2068..2091
                     /label="linker2"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     CDS             2098..2985
                     /label="Msn2 activation domain"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     terminator      3008..3232
                     /label="ScADH1 Terminator"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     misc_feature    3248..3390
                     /label="ConRE"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     terminator      complement(3418..3514)
                     /label="AmpR Terminator"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     CDS             complement(3515..4372)
                     /label="AmpR"
                     /ApEinfo_revcolor="#9f4240"
                     /ApEinfo_fwdcolor="#9f4240"
     promoter        complement(4373..4503)
                     /label="AmpR Promoter"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     rep_origin      complement(4504..5267)
                     /label="ColE1"
                     /ApEinfo_revcolor="#7f7f7f"
                     /ApEinfo_fwdcolor="#7f7f7f"
ORIGIN
        1 gaattcgcat ctagaacggg gtcatcacgg ctcatcatgc gccaaacaaa tgtgtgcaat
       61 acacgctcgg atgactgcat gatgaccgca ctgactgggg acagcagatc cacctaagcc
      121 tgtgagagaa gcagacaccc gacagatcaa ggcagttacg tctcaccaaa ccagatgtca
      181 acacagctac aacgccttgc caacagggag ttcttcagag acatggaggc tcaaaacgaa
      241 attattgaca gcctagacat caatagtcat acaacagaaa gcgaccaccc aactttggct
      301 gataatagcg tataaacaat gcatactttg tacgttcaaa atacaatgca gtagatatat
      361 ttatgcatat tacatataat acatatcaca taggaagcaa caggcgcgtt ggacttttaa
      421 ttttcgagga ccgcgaatcc ttacatcaca cccaatcccc cacaagtgat cccccacaca
      481 ccatagcttc aaaatgtttc tactcctttt ttactcttcc agattttctc ggactccgcg
      541 catcgccgta ccacttcaaa acacccaagc acagcatact aaatttcccc tctttcttcc
      601 tctagggtgt cgttaattac ccgtactaaa ggtttggaaa agaaaaaaga caccgcctcg
      661 tttctttttc ttcgtcgaaa aaggcaataa aaatttttat cacgtttctt tttcttgaaa
      721 attttttttt ttgatttttt tctctttcga tgacctccca ttgatattta agttaataaa
      781 cggtcatcaa tttctcaagt ttcagtttca tttttcttgt tctattacaa ctttttttac
      841 ttcttgctca ttagaaagaa agcatagcaa tctaatctaa gttttaatta caaaagatcT
      901 ATGCTCGAGA AAAAAatgga gcgccccttc cagtgtcgca tttgcatgcg gaacttttcg
      961 gattctccaa ctttgagaag acatacccgt actcataccg gtgaaaaacc gtttcagtgt
     1021 cggatctgta tgcgaaattt ctcccaaaga tcatctttgg ttagacatct acgtacgcac
     1081 accggcgaga agccattcca atgccgaata tgcatgcgca acttcagtga aagaggtaat
     1141 ttgactagac acctaaaaac ccacctgaga ggagatggtg ctggattgat tgatATCGAT
     1201 ctgaagaaac ttggtaatct gaaactacag gaggaaggag aggcttccag caccaccagc
     1261 cccactgagg agacaaccca gaagctgaca gtgtcacaca ttgaaggcta tgaatgtcag
     1321 cccatctttc tgaatgtcct ggaagccatt gagccaggtg tagtgtgtgc tggacacgac
     1381 aacaaccagc ccgactcctt tgcagccttg ctctctagcc tcaatgaact gggagagaga
     1441 cagcttgtac acgtggtcaa gtgggccaag gccttgcctg gcttccgcaa cttacacgtg
     1501 gacgaccaga tggctgtcat tcagtactcc tggatggggc tcatggtgtt tgccatgggc
     1561 tggcgatcct tcaccaatgt caactccagg atgctctact tcgcccctga tctggttttc
     1621 aatgagtacc gcatgcacaa gtcccggatg tacagccagt gtgtccgaat gaggcacctc
     1681 Cctcaagagt ttggatggct ccaaatcacc ccccaggaat tcctgtgcat gaaagcactg
     1741 ctactcttca gcattattcc agtggatggg ctgaaaaatc aaaaattctt tgatgaactt
     1801 cgaatgaact acatcaagga actcgatcgt atcattgcat gcaaaagaaa aaatcccaca
     1861 tcctgctcaa gacgcttcta ccagctcacc aagctcctgg actccgtgca gcctattgcg
     1921 agagagctgc atcagttcac ttttgacctg ctaatcaagt cacacatggt gagcgtggac
     1981 tttccggaaa tgatggcaga gatcatctct gtgcaagtgc ccaagatcct ttctgggaaa
     2041 gtcaagccca tctatttcca cacccagggc gacggagcag gcttaataaa tGCTAGCatg
     2101 acggtcgacc atgatttcaa tagcgaagat attttattcc ccatagaaag catgagtagt
     2161 atacaatacg tggagaataa taacccaaat aatattaaca acgatgttat cccgtattct
     2221 ctagatatca aaaacactgt cttagatagt gcggatctca atgacattca aaatcaagaa
     2281 acttcactga atttggggct tcctccacta tctttcgact ctccactgcc cgtaacggaa
     2341 acgataccat ccactaccga taacagcttg catttgaaag ctgatagcaa caaaaatcgc
     2401 gatgcaagaa ctattgaaaa tgatagtgaa attaagagta ctaataatgc tagtggctct
     2461 ggggcaaatc aatacacaac tcttacttca ccttatccta tgaacgacat tttgtacaac
     2521 atgaacaatc cgttacaatc accgtcacct tcatcggtac ctcaaaatcc gactataaat
     2581 cctcccataa atacagcaag taacgaaact aatttatcgc ctcaaacttc aaatggtaat
     2641 gaaactctta tatctccacg agcccaacaa catacgtcca ttaaagataa tcgtctgtcc
     2701 ttacctaatg gtgctaattc gaatcttttc attgacacta acccaaacaa tttgaacgaa
     2761 aaactaagaa atcaattgaa ctcagataca aattcatatt ctaactccat ttctaattca
     2821 aactccaatt ctacgggtaa tttaaattcc agttatttta attcactgaa catagactcc
     2881 atgctagatg attacgtttc tagtgatctc ttattgaatg atgatgatga tgacactaat
     2941 ttatcacgcc gaagatttag cgacgttata acaaaccaag agctcTAAGG GCCCatccta
     3001 actcgaggcg aatttcttat gatttatgat ttttattatt aaataagtta taaaaaaaat
     3061 aagtgtatac aaattttaaa gtgactctta ggttttaaaa cgaaaattct tattcttgag
     3121 taactctttc ctgtaggtca ggttgctttc tcaggtatag catgaggtcg ctcttattga
     3181 ccacacctct accggcatgc cgagcaaatg cctgcaaatc gctccccatt tcgctgagca
     3241 tgagacggaa atctgctcgt cagtggtgct cacactgacg aatcatgtac agatcatacc
     3301 gatgactgcc tggcgactca caactaagca agacagccgg aaccagcgcc ggcgaacacc
     3361 actgcatata tggcatatca caacagtcca actagtgcac tgcagtacag cggccgcgat
     3421 tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct
     3481 aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta
     3541 tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa
     3601 ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg cgggacccac
     3661 gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa
     3721 gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag
     3781 taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctaca ggcatcgtgg
     3841 tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag
     3901 ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg
     3961 tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc
     4021 ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat
     4081 tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata cgggataata
     4141 ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa
     4201 aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca
     4261 actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc
     4321 aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc
     4381 tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg
     4441 aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac
     4501 ctgtcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag
     4561 aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa
     4621 caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt
     4681 ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgttctt ctagtgtagc
     4741 cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa
     4801 tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa
     4861 gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc
     4921 ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa
     4981 gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa
     5041 caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg
     5101 ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc
     5161 tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg
     5221 ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtgcg gccgcccct
//