LOCUS       pAS163_(synMR_Cassette) 5369 bp ds-DNA     circular     15-NOV-2022
DEFINITION  .
KEYWORDS    "Selection:Amp"
FEATURES             Location/Qualifiers
     misc_feature    16..158
                     /label="ConL1"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     misc_feature    166..190
                     /label="ConL1 scar"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     promoter        195..894
                     /label="ScTEF1 Promoter"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     RBS             910..915
                     /label="Kozak"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     CDS             916..1179
                     /label="Zif268"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
     CDS             1180..1203
                     /label="linker1"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     CDS             1210..2157
                     /label="MR LBD"
                     /ApEinfo_revcolor="#0080ff"
                     /ApEinfo_fwdcolor="#0080ff"
     misc_feature    1613..1613
                     /label="A->G"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     CDS             2158..2181
                     /label="linker2"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     CDS             2188..3075
                     /label="Msn2 activation domain"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     terminator      3098..3322
                     /label="ScADH1 Terminator"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     misc_feature    3338..3480
                     /label="ConRE"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     terminator      complement(3508..3604)
                     /label="AmpR Terminator"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     CDS             complement(3605..4462)
                     /label="AmpR"
                     /ApEinfo_revcolor="#9f4240"
                     /ApEinfo_fwdcolor="#9f4240"
     promoter        complement(4463..4593)
                     /label="AmpR Promoter"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     rep_origin      complement(4594..5357)
                     /label="ColE1"
                     /ApEinfo_revcolor="#7f7f7f"
                     /ApEinfo_fwdcolor="#7f7f7f"
ORIGIN
        1 gaattcgcat ctagaacggg gtcatcacgg ctcatcatgc gccaaacaaa tgtgtgcaat
       61 acacgctcgg atgactgcat gatgaccgca ctgactgggg acagcagatc cacctaagcc
      121 tgtgagagaa gcagacaccc gacagatcaa ggcagttacg tctcaccaaa ccagatgtca
      181 acacagctac aacgccttgc caacagggag ttcttcagag acatggaggc tcaaaacgaa
      241 attattgaca gcctagacat caatagtcat acaacagaaa gcgaccaccc aactttggct
      301 gataatagcg tataaacaat gcatactttg tacgttcaaa atacaatgca gtagatatat
      361 ttatgcatat tacatataat acatatcaca taggaagcaa caggcgcgtt ggacttttaa
      421 ttttcgagga ccgcgaatcc ttacatcaca cccaatcccc cacaagtgat cccccacaca
      481 ccatagcttc aaaatgtttc tactcctttt ttactcttcc agattttctc ggactccgcg
      541 catcgccgta ccacttcaaa acacccaagc acagcatact aaatttcccc tctttcttcc
      601 tctagggtgt cgttaattac ccgtactaaa ggtttggaaa agaaaaaaga caccgcctcg
      661 tttctttttc ttcgtcgaaa aaggcaataa aaatttttat cacgtttctt tttcttgaaa
      721 attttttttt ttgatttttt tctctttcga tgacctccca ttgatattta agttaataaa
      781 cggtcatcaa tttctcaagt ttcagtttca tttttcttgt tctattacaa ctttttttac
      841 ttcttgctca ttagaaagaa agcatagcaa tctaatctaa gttttaatta caaaagatcT
      901 ATGCTCGAGA AAAAAatggg tacccgccca tatgcttgcc ctgtcgagtc ctgcgatcgc
      961 cgcttttctc gctcggatga gcttacccgc catatccgca tccataccgg tcagaagccc
     1021 ttccagtgtc gaatctgcat gcgtaacttc agtcgtagtg accaccttac cacccacatc
     1081 cgcacccaca caggcgagaa gccttttgcc tgtgacattt gtgggaggaa gtttgccagg
     1141 agtgatgaac gcaagaggca taccaaaatc catacaggtg gagatggtgc tggattgatt
     1201 gatATCGATa atttaggagc acgaaagtca aagaagttgg gaaagttaaa agggattcac
     1261 gaggagcagc cacagcagca gcagccccca cccccacccc cacccccgca aagcccagag
     1321 gaagggacaa cgtacatcgc tcctgcaaaa gaaccctcgg tcaacacagc actggttcct
     1381 cagctctcca caatctcacg agcgctcaca ccttcccccg ttatggtcct tgaaaacatt
     1441 gaacctgaaa ttgtatatgc aggctatgac agctcaaaac cagatacagc cgaaaatctg
     1501 ctctccacgc tcaaccgctt agcaggcaaa cagatgatcc aagtcgtgaa gtgggcaaag
     1561 gtacttccag gatttaaaaa cttgcctctt gaggaccaaa ttaccctaat ccGgtattct
     1621 tggatgtgtc tatcatcatt tgccttgagc tggagatcgt acaaacatac gaacagccaa
     1681 tttctctatt ttgcaccaga cctagtcttt aatgaagaga agatgcatca gtctgccatg
     1741 tatgaactat gccaggggat gcaccaaatc agccttcagt tcgttcgact gcagctcacc
     1801 tttgaagaat acaccatcat gaaagttttg ctgctactaa gcacaattcc aaaggatggc
     1861 ctcaaaagcc aggctgcatt tgaagaaatg aggacaaatt acatcaaaga actgaggaag
     1921 atggtaacta agtgtcccaa caattctggg cagagctggc agaggttcta ccaactgacc
     1981 aagctgctgg actccatgca tgacctggtg agcgacctgc tggaattctg cttctacacc
     2041 ttccgagagt cccatgcgct gaaggtagag ttccccgcaa tgctggtgga gatcatcagc
     2101 gaccagctgc ccaaggtgga gtcggggaac gccaagccgc tctacttcca ccggaagggc
     2161 gacggagcag gcttaataaa tGCTAGCatg acggtcgacc atgatttcaa tagcgaagat
     2221 attttattcc ccatagaaag catgagtagt atacaatacg tggagaataa taacccaaat
     2281 aatattaaca acgatgttat cccgtattct ctagatatca aaaacactgt cttagatagt
     2341 gcggatctca atgacattca aaatcaagaa acttcactga atttggggct tcctccacta
     2401 tctttcgact ctccactgcc cgtaacggaa acgataccat ccactaccga taacagcttg
     2461 catttgaaag ctgatagcaa caaaaatcgc gatgcaagaa ctattgaaaa tgatagtgaa
     2521 attaagagta ctaataatgc tagtggctct ggggcaaatc aatacacaac tcttacttca
     2581 ccttatccta tgaacgacat tttgtacaac atgaacaatc cgttacaatc accgtcacct
     2641 tcatcggtac ctcaaaatcc gactataaat cctcccataa atacagcaag taacgaaact
     2701 aatttatcgc ctcaaacttc aaatggtaat gaaactctta tatctccacg agcccaacaa
     2761 catacgtcca ttaaagataa tcgtctgtcc ttacctaatg gtgctaattc gaatcttttc
     2821 attgacacta acccaaacaa tttgaacgaa aaactaagaa atcaattgaa ctcagataca
     2881 aattcatatt ctaactccat ttctaattca aactccaatt ctacgggtaa tttaaattcc
     2941 agttatttta attcactgaa catagactcc atgctagatg attacgtttc tagtgatctc
     3001 ttattgaatg atgatgatga tgacactaat ttatcacgcc gaagatttag cgacgttata
     3061 acaaaccaag agctcTAAGG GCCCatccta actcgaggcg aatttcttat gatttatgat
     3121 ttttattatt aaataagtta taaaaaaaat aagtgtatac aaattttaaa gtgactctta
     3181 ggttttaaaa cgaaaattct tattcttgag taactctttc ctgtaggtca ggttgctttc
     3241 tcaggtatag catgaggtcg ctcttattga ccacacctct accggcatgc cgagcaaatg
     3301 cctgcaaatc gctccccatt tcgctgagca tgagacggaa atctgctcgt cagtggtgct
     3361 cacactgacg aatcatgtac agatcatacc gatgactgcc tggcgactca caactaagca
     3421 agacagccgg aaccagcgcc ggcgaacacc actgcatata tggcatatca caacagtcca
     3481 actagtgcac tgcagtacag cggccgcgat tatcaaaaag gatcttcacc tagatccttt
     3541 taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca
     3601 gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca
     3661 tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc
     3721 ccagtgctgc aatgataccg cgggacccac gctcaccggc tccagattta tcagcaataa
     3781 accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc
     3841 agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca
     3901 acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat
     3961 tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag
     4021 cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac
     4081 tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt
     4141 ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt
     4201 gctcttgccc ggcgtcaata cgggataata ccgcgccaca tagcagaact ttaaaagtgc
     4261 tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat
     4321 ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca
     4381 gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga
     4441 cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg
     4501 gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg
     4561 ttccgcgcac atttccccga aaagtgccac ctgtcatgac caaaatccct taacgtgagt
     4621 tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt
     4681 tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt
     4741 gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc
     4801 agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg
     4861 tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg
     4921 ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt
     4981 cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac
     5041 tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg
     5101 acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg
     5161 gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat
     5221 ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt
     5281 tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg
     5341 attctgtgga taaccgtgcg gccgcccct
//