LOCUS       pAS165_(97-4_Op_(Part_2 2090 bp ds-DNA     circular     15-NOV-2022
DEFINITION  .
KEYWORDS    "Selection:Cam"
FEATURES             Location/Qualifiers
     enhancer        20..194
                     /label="7x 97-4 operator"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     misc_feature    34..42
                     /label="ZF 97-4 op"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     misc_feature    57..65
                     /label="ZF 97-4 op"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     misc_feature    80..88
                     /label="ZF 97-4 op"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     misc_feature    103..111
                     /label="ZF 97-4 op"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     misc_feature    126..134
                     /label="ZF 97-4 op"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     misc_feature    149..157
                     /label="ZF 97-4 op"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     misc_feature    172..180
                     /label="ZF 97-4 op"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     promoter        201..437
                     /label="pCYC1"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     misc_feature    complement(451..559)
                     /label="CamR Terminator"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     CDS             complement(560..1219)
                     /label="CamR"
                     /ApEinfo_revcolor="#0000ff"
                     /ApEinfo_fwdcolor="#0000ff"
     promoter        complement(1220..1324)
                     /label="CamR Promoter"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     rep_origin      complement(1325..2088)
                     /label="ColE1"
                     /ApEinfo_revcolor="#7f7f7f"
                     /ApEinfo_fwdcolor="#7f7f7f"
ORIGIN
        1 TCGGTCTCAA ACGCCTAGGT TGAGGCCAAC GtaTTATGGG AGatcgaata ttaataTTAT
       61 GGGAGatcga atattaataT TATGGGAGat cgaatattaa taTTATGGGA Gatcgaatat
      121 taataTTATG GGAGatcgaa tattaataTT ATGGGAGatc gaatattaat aTTATGGGAG
      181 aTGGCATGAA TGGCCTTAAG cagatccgcc aggcgtgtat atatagcgtg gatggccagg
      241 caactttagt gctgacacat acaggcatat atatatgtgt gcgacgacac atgatcatat
      301 ggcatgcatg tgctctgtat gtatataaaa ctcttgtttt cttcttttct ctaaatattc
      361 tttccttata cattaggacc tttgcagcat aaattactat acttctatag acacacaaac
      421 acaaatacac acactaaTAT GTGAGACCag accaataaaa aacgcccggc ggcaaccgag
      481 cgttctgaac aaatccagat ggagttctga ggtcattact ggatctatca acaggagtcc
      541 aagcgagctc gatatcaaat tacgccccgc cctgccactc atcgcagtac tgttgtaatt
      601 cattaagcat tctgccgaca tggaagccat cacaaacggc atgatgaacc tgaatcgcca
      661 gcggcatcag caccttgtcg ccttgcgtat aatatttgcc catggtgaaa acgggggcga
      721 agaagttgtc catattggcc acgtttaaat caaaactggt gaaactcacc cagggattgg
      781 ctgaaacgaa aaacatattc tcaataaacc ctttagggaa ataggccagg ttttcaccgt
      841 aacacgccac atcttgcgaa tatatgtgta gaaactgccg gaaatcgtcg tggtattcac
      901 tccagagcga tgaaaacgtt tcagtttgct catggaaaac ggtgtaacaa gggtgaacac
      961 tatcccatat caccagctca ccgtctttca ttgccatacg aaattccgga tgagcattca
     1021 tcaggcgggc aagaatgtga ataaaggccg gataaaactt gtgcttattt ttctttacgg
     1081 tctttaaaaa ggccgtaata tccagctgaa cggtctggtt ataggtacat tgagcaactg
     1141 actgaaatgc ctcaaaatgt tctttacgat gccattggga tatatcaacg gtggtatatc
     1201 cagtgatttt tttctccatt ttagcttcct tagctcctga aaatctcgat aactcaaaaa
     1261 atacgcccgg tagtgatctt atttcattat ggtgaaagtt ggaacctctt acgtgcccga
     1321 tcaatcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta
     1381 gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa
     1441 acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt
     1501 tttccgaagg taactggctt cagcagagcg cagataccaa atactgttct tctagtgtag
     1561 ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta
     1621 atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca
     1681 agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag
     1741 cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa
     1801 agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga
     1861 acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc
     1921 gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc
     1981 ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt
     2041 gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtag
//