LOCUS       pTarg_cLap_LNGFR        5221 bp    DNA        circular     16-NOV-2022
DEFINITION  
ACCESSION   
VERSION     
SOURCE      
  ORGANISM  
COMMENT     
COMMENT     
COMMENT     ApEinfo:methylated:1
FEATURES             Location/Qualifiers
     primer_bind     2476..2491
                     /vntifkey="28"
                     /locus_tag="M13rev"
                     /label="M13rev"
                     /ApEinfo_label="M13rev"
                     /ApEinfo_fwdcolor="pink"
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    754..1038
                     /locus_tag="NGFP-exon1"
                     /label="NGFP-exon1"
                     /ApEinfo_label="NGFP-exon1"
                     /ApEinfo_fwdcolor="#2bff27"
                     /ApEinfo_revcolor="#2bff27"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    342..360
                     /locus_tag="CRISPR"
                     /label="CRISPR"
                     /ApEinfo_label="CRISPR"
                     /ApEinfo_fwdcolor="#66ff66"
                     /ApEinfo_revcolor="#ff6666"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    1039..1470
                     /locus_tag="NGFP-exon2"
                     /label="NGFP-exon2"
                     /ApEinfo_label="NGFP-exon2"
                     /ApEinfo_fwdcolor="#17ff51"
                     /ApEinfo_revcolor="#17ff51"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    337..340
                     /locus_tag="Overhang 3"
                     /label="Overhang 3"
                     /ApEinfo_label="Overhang 3"
                     /ApEinfo_fwdcolor="#ff80c0"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    1473..1531
                     /locus_tag="P2A"
                     /label="P2A"
                     /ApEinfo_label="P2A"
                     /ApEinfo_fwdcolor="#ff8000"
                     /ApEinfo_revcolor="#ff0000"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    101..332
                     /locus_tag="hU6"
                     /label="hU6"
                     /ApEinfo_label="hU6"
                     /ApEinfo_fwdcolor="#0000ff"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     primer_bind     3..24
                     /vntifkey="28"
                     /locus_tag="M13\for"
                     /label="M13\for"
                     /ApEinfo_label="M13\for"
                     /ApEinfo_fwdcolor="pink"
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     polyA_signal    64..65
                     /vntifkey="25"
                     /locus_tag="bpA"
                     /label="bpA"
                     /ApEinfo_label="bpA"
                     /ApEinfo_fwdcolor="pink"
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    516..518
                     /locus_tag="Pam"
                     /label="Pam"
                     /ApEinfo_label="Pam"
                     /ApEinfo_fwdcolor="#999999"
                     /ApEinfo_revcolor="#999999"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    1534..2373
                     /note="LNGFR open reading frame"
                     /locus_tag="LNGFR"
                     /label="LNGFR"
                     /ApEinfo_label="LNGFR"
                     /ApEinfo_fwdcolor="#800080"
                     /ApEinfo_revcolor="#800080"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     primer_bind     complement(2439..2458)
                     /locus_tag="T3"
                     /label="T3"
                     /ApEinfo_label="T3"
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    2380..2399
                     /locus_tag="CRISPR(1)"
                     /label="CRISPR(1)"
                     /ApEinfo_label="CRISPR"
                     /ApEinfo_fwdcolor="#66ff66"
                     /ApEinfo_revcolor="#ff6666"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    72..89
                     /vntifkey="21"
                     /locus_tag="I-Sce"
                     /label="I-Sce"
                     /ApEinfo_label="I-Sce"
                     /ApEinfo_fwdcolor="#cccccc"
                     /ApEinfo_revcolor="#cccccc"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     primer_bind     complement(2476..2496)
                     /locus_tag="M13-rev"
                     /label="M13-rev"
                     /ApEinfo_label="M13-rev"
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     primer_bind     7..24
                     /locus_tag="M13-fwd"
                     /label="M13-fwd"
                     /ApEinfo_label="M13-fwd"
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     primer_bind     33..53
                     /locus_tag="T7"
                     /label="T7"
                     /ApEinfo_label="T7"
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     rep_origin      complement(2864..3546)
                     /locus_tag="ColE1 origin"
                     /label="ColE1 origin"
                     /ApEinfo_label="ColE1 origin"
                     /ApEinfo_fwdcolor="gray50"
                     /ApEinfo_revcolor="gray50"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     rep_origin      complement(4764..5070)
                     /locus_tag="F1 ori"
                     /label="F1 ori"
                     /ApEinfo_label="F1 ori"
                     /ApEinfo_fwdcolor="gray50"
                     /ApEinfo_revcolor="gray50"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     CDS             complement(5089..5157)
                     /locus_tag="LacZ alpha"
                     /label="LacZ alpha"
                     /ApEinfo_label="LacZ alpha"
                     /ApEinfo_fwdcolor="#6495ed"
                     /ApEinfo_revcolor="#6495ed"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_binding    complement(2502..2524)
                     /locus_tag="LacO"
                     /label="LacO"
                     /ApEinfo_label="LacO"
                     /ApEinfo_fwdcolor="#6495ed"
                     /ApEinfo_revcolor="#6495ed"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     CDS             complement(3644..4303)
                     /locus_tag="AmpR"
                     /label="AmpR"
                     /ApEinfo_label="AmpR"
                     /ApEinfo_fwdcolor="yellow"
                     /ApEinfo_revcolor="yellow"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    361..364
                     /locus_tag="Overhang 5"
                     /label="Overhang 5"
                     /ApEinfo_label="Overhang 5"
                     /ApEinfo_fwdcolor="#ff80c0"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     polyA_signal    2411..2412
                     /vntifkey="25"
                     /locus_tag="bpA(1)"
                     /label="bpA(1)"
                     /ApEinfo_label="bpA"
                     /ApEinfo_fwdcolor="pink"
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     protein_bind    547..570
                     /locus_tag="FLAG(hum.adopt)"
                     /label="FLAG(hum.adopt)"
                     /ApEinfo_label="FLAG(hum.adopt)"
                     /ApEinfo_fwdcolor="#ff2834"
                     /ApEinfo_revcolor="#ff2834"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     protein_bind    622..663
                     /locus_tag="S-pept(hum.adopt)"
                     /label="S-pept(hum.adopt)"
                     /ApEinfo_label="S-pept(hum.adopt)"
                     /ApEinfo_fwdcolor="#009a9a"
                     /ApEinfo_revcolor="#009a9a"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    697..732
                     /locus_tag="PreScission (hum.adopt)"
                     /label="PreScission (hum.adopt)"
                     /ApEinfo_label="PreScission (hum.adopt)"
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="cyan"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    586..606
                     /locus_tag="TEV(hum.adopt)"
                     /label="TEV(hum.adopt)"
                     /ApEinfo_label="TEV(hum.adopt)"
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="cyan"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    496..515
                     /locus_tag="CRISPR(2)"
                     /label="CRISPR(2)"
                     /ApEinfo_label="CRISPR"
                     /ApEinfo_fwdcolor="#66ff66"
                     /ApEinfo_revcolor="#ff6666"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    2400..2402
                     /locus_tag="Pam(1)"
                     /label="Pam(1)"
                     /ApEinfo_label="Pam"
                     /ApEinfo_fwdcolor="#999999"
                     /ApEinfo_revcolor="#999999"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
ORIGIN
        1 cgacgttgta aaacgacggc cagtgagcgc gcgtaatacg actcactata gggcgaattg
       61 gagctaCCCG Cattaccctg ttatccctaA GATCCgccta tttcccatga ttccttcata
      121 tttgcatata cgatacaagg ctgttagaga gataattaga attaatttga ctgtaaacac
      181 aaagatatta gtacaaaata cgtgacgtag aaagtaataa tttcttgggt agtttgcagt
      241 tttaaaatta tgttttaaaa tggactatca tatgcttacc gtaacttgaa agtatttcga
      301 tttcttggct ttatatatct tGTGGAAAGG ACGAAACACC ggtatgtcgg gaacctctcc
      361 gttttagagc taGAAAtagc aagttaaaat aaggctagtc cgttatcaac ttgaaaaagt
      421 ggcaccgagt cggtgcTTTT TTgaattcAG ATCTTACatt tcaggacatc tagcttatcg
      481 ataccgtcGA CTACaggtat gtcgggaacc tctccaggaC TGACCGACGA CCTGCCCAGG
      541 GCCTCGGACT ATAAGGACGA CGACGACAAG GGCGGAGGCG GAGGGGAGAA CCTGTACTTT
      601 CAGGGAAGCG GAGGAGGAGG CAAAGAAACA GCTGCTGCTA AATTCGAGCG GCAGCACATG
      661 GACAGCTCTG GCCCCTCTGG CAGCGCCGCC AAGTTCAGCA GCCTGGAAGT GCTGTTCCAG
      721 GGCCCCCTGA GCAGCAGCGG CCCCAGCGGC AGCATGGTGT CCAAGGGCGA GGAACTGTTC
      781 ACCGGCGTGG TGCCCATCCT GGTGGAGCTG GACGGCGACG TGAACGGCCA CAAGTTCAGC
      841 GTGTCCGGCG AGGGCGAAGG CGACGCCACC TACGGCAAGC TGACCCTGAA GTTCATCTGC
      901 ACCACCGGCA AGCTGCCCGT GCCCTGGCCC ACCCTGGTGA CCACCCTGAC CTACGGCGTG
      961 CAGTGCTTCA GCAGATACCC CGACCACATG AAGCAGCACG ATTTCTTCAA GAGCGCCATG
     1021 CCCGAGGGCT ACGTCCAggA GCGCACCATC TTTTTCAAGG ACGACGGCAA CTACAAGACC
     1081 AGGGCCGAGG TGAAGTTCGA GGGCGACACA CTGGTGAACA GGATCGAGCT GAAGGGCATC
     1141 GACTTCAAAG AGGACGGCAA CATCCTGGGC CACAAGCTGG AATACAACTA CAACAGCCAC
     1201 AACGTGTACA TTATGGCCGA CAAGCAGAAG AACGGCATCA AGGTCAACTT CAAGATCCGG
     1261 CACAACATCG AGGACGGCAG CGTGCAGCTG GCCGACCACT ACCAGCAGAA CACCCCCATC
     1321 GGCGACGGCC CCGTGCTGCT GCCCGACAAC CACTACCTGA GCACCCAGAG CGCTCTCAGC
     1381 AAGGACCCCA ACGAGAAGCG GGACCACATG GTGCTGAAAG AATTTGTGAC AGCCGCCGGA
     1441 ATCACCCTGG GCATGGACGA GCTGTACAAG cgCGGAGCCA CGAACTTCTC TCTGTTAAAG
     1501 CAAGCAGGAG ATGTTGAAGA AAACCCCGGG Cccatggggg caggtgccac cggccgcgcc
     1561 atggacgggc cgcgcctgct gctgttgctg cttctggggg tgtcccttgg aggtgccaag
     1621 gaggcatgcc ccacaggcct gtacacacac agcggtgagt gctgcaaagc ctgcaacctg
     1681 ggcgagggtg tggcccagcc ttgtggagcc aaccagaccg tgtgtgagcc ctgcctggac
     1741 agcgtgacgt tctccgacgt ggtgagcgcg accgagccgt gcaagccgtg caccgagtgc
     1801 gtggggctcc agagcatgtc ggcgccgtgc gtggaggccg acgacgccgt gtgccgctgc
     1861 gcctacggct actaccagga tgagacgact gggcgctgcg aggcgtgccg cgtgtgcgag
     1921 gcgggctcgg gcctcgtgtt ctcctgccag gacaagcaga acaccgtgtg cgaggagtgc
     1981 cccgacggca cctattccga cgaggccaac cacgtggacc cgtgcctgcc ctgcaccgtg
     2041 tgcgaggaca ccgagcgcca gctccgcgag tgcacacgct gggccgacgc cgagtgcgag
     2101 gagatccctg gccgttggat tacacggtcc acacccccag agggctcgga cagcacagcc
     2161 cccagcaccc aggagcctga ggcacctcca gaacaagacc tcatagccag cacggtggca
     2221 ggtgtggtga ccacagtgat gggcagctcc cagcccgtgg tgacccgagg caccaccgac
     2281 aacctcatcc ctgtctattg ctccatcctg gctgctgtgg ttgtgggcct tgtggcctac
     2341 atagccttca agaggtggaa cagggggatc ctcTGAcctg gtatgtcggg aacctctcca
     2401 gggatgtCTC gagggggggc ccggtaccca gcttttgttc cctttagtga gggttaattg
     2461 cgcgcttggc gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa
     2521 ttccacacaa catacgagcc ggaagcataa agtgtaaagc ctggggtgcc taatgagtga
     2581 gctaactcac attaattgcg ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt
     2641 gccagctgca ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt attgggcgct
     2701 cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat
     2761 cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga
     2821 acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt
     2881 ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt
     2941 ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc
     3001 gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa
     3061 gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct
     3121 ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta
     3181 actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg
     3241 gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc
     3301 ctaactacgg ctacactaga aggacagtat ttggtatctg cgctctgctg aagccagtta
     3361 ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg
     3421 gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt
     3481 tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg
     3541 tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta
     3601 aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg
     3661 aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg
     3721 tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc
     3781 gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg
     3841 agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg
     3901 aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag
     3961 gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat
     4021 caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc
     4081 cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc
     4141 ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa
     4201 ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac
     4261 gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt
     4321 cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc
     4381 gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa
     4441 caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca
     4501 tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat
     4561 acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa
     4621 aagtgccacc taaattgtaa gcgttaatat tttgttaaaa ttcgcgttaa atttttgtta
     4681 aatcagctca ttttttaacc aataggccga aatcggcaaa atcccttata aatcaaaaga
     4741 atagaccgag atagggttga gtgttgttcc agtttggaac aagagtccac tattaaagaa
     4801 cgtggactcc aacgtcaaag ggcgaaaaac cgtctatcag ggcgatggcc cactacgtga
     4861 accatcaccc taatcaagtt ttttggggtc gaggtgccgt aaagcactaa atcggaaccc
     4921 taaagggagc ccccgattta gagcttgacg gggaaagccg gcgaacgtgg cgagaaagga
     4981 agggaagaaa gcgaaaggag cgggcgctag ggcgctggca agtgtagcgg tcacgctgcg
     5041 cgtaaccacc acacccgccg cgcttaatgc gccgctacag ggcgcgtccc attcgccatt
     5101 caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat tacgccagct
     5161 ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt tttcccagtc
     5221 a     
//
