LOCUS       Exported                5214 bp ds-DNA     circular SYN 29-JUN-2022
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5214)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Wednesday, Nov 16, 2022 from SnapGene 5.0.8
            https://www.snapgene.com
COMMENT     Annotated with pLannotate v1.2.0
FEATURES             Location/Qualifiers
     source          1..5214
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     rep_origin      904..1492
                     /locus_tag="ori"
                     /label=ori
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=99.7"
                     /note="/match_length=100.0"
                     /note="/other=rep_origin"
     CDS             1675..1767
                     /locus_tag="lacI (fragment)"
                     /label=lacI (fragment)
                     /note="pLannotate"
                     /note="/ApEinfo_hidden="
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=8.6"
                     /note="/other=CDS"
     protein_bind    1780..1801
                     /locus_tag="CAP binding site"
                     /label=CAP binding site
                     /note="pLannotate"
                     /note="/ApEinfo_hidden="
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=protein_bind"
     promoter        1816..1846
                     /locus_tag="lac promoter"
                     /label=lac promoter
                     /note="pLannotate"
                     /note="/ApEinfo_hidden="
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=promoter"
     protein_bind    1854..1870
                     /locus_tag="lac operator"
                     /label=lac operator
                     /note="pLannotate"
                     /note="/ApEinfo_hidden="
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=protein_bind"
     promoter        1915..1933
                     /locus_tag="T3 promoter"
                     /label=T3 promoter
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=promoter"
     misc_feature    1946..2053
                     /locus_tag="MCS"
                     /label=MCS
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=misc_feature"
     promoter        complement(2062..2080)
                     /locus_tag="T7 promoter"
                     /label=T7 promoter
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=promoter"
     CDS             2088..2243
                     /locus_tag="lacZ-alpha (fragment)"
                     /label=lacZ-alpha (fragment)
                     /note="pLannotate"
                     /note="/ApEinfo_hidden="
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=89.7"
                     /note="/other=CDS"
     rep_origin      2247..2675
                     /locus_tag="f1 ori"
                     /label=f1 ori
                     /note="pLannotate"
                     /note="/ApEinfo_hidden="
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=rep_origin"
     misc_feature    complement(2703..2754)
                     /locus_tag="bom (fragment)"
                     /label=bom (fragment)
                     /note="pLannotate"
                     /note="/ApEinfo_hidden="
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=36.9"
                     /note="/other=misc_feature"
     gene            complement(2790..4146)
                     /locus_tag="kanMX"
                     /label=kanMX
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=gene"
     misc_feature    complement(4189..4238)
                     /locus_tag="bom (fragment)(1)"
                     /label=bom (fragment)
                     /label=bom (fragment)(1)
                     /note="pLannotate"
                     /note="/ApEinfo_hidden="
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=35.5"
                     /note="/other=misc_feature"
     CDS             complement(4340..4375)
                     /locus_tag="rop (fragment)"
                     /label=rop (fragment)
                     /note="pLannotate"
                     /note="/ApEinfo_hidden="
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=18.8"
                     /note="/other=CDS"
     misc_feature    complement(4443..4945)
                     /locus_tag="CEN/ARS"
                     /label=CEN/ARS
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=99.8"
                     /note="/match_length=100.0"
                     /note="/other=misc_feature"
     promoter        4982..5086
                     /locus_tag="AmpR promoter"
                     /label=AmpR promoter
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=promoter"
     CDS             join(5087..5214,1..733)
                     /locus_tag="AmpR"
                     /label=AmpR
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=99.8"
                     /note="/match_length=100.0"
                     /note="/other=CDS"
ORIGIN
        1 ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg
       61 ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtattga
      121 cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta
      181 ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc
      241 tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc
      301 gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg
      361 ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgtagc
      421 aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca
      481 acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct
      541 tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat
      601 cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg
      661 gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat
      721 taagcattgg taactgtcag accaagttta ctcatatata ctttagattg atttaaaact
      781 tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat
      841 cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc
      901 ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct
      961 accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg
     1021 cttcagcaga gcgcagatac caaatactgt tcttctagtg tagccgtagt taggccacca
     1081 cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc
     1141 tgctgccagt ggcgataagt cgtgtcttac caggttggac tcaagacgat agttaccgga
     1201 taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac
     1261 gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga
     1321 agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag
     1381 ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg
     1441 acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag
     1501 caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc
     1561 tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc
     1621 tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgccc
     1681 aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat taatgcagct ggcacgacag
     1741 gtttcccgac tggaaagcgg gcagtgagcg caacgcaatt aatgtgagtt agctcactca
     1801 ttaggcaccc caggctttac actttatgct tccggctcgt atgttgtgtg gaattgtgag
     1861 cggataacaa tttcacacag gaaacagcta tgaccatgat tacgccaagc gcgcaattaa
     1921 ccctcactaa agggaacaaa agctggagct ccaccgcggt ggcggccgct ctagaactag
     1981 tggatccccc gggctgcagg aattcgatat caagcttatc gataccgtcg acctcgaggg
     2041 ggggcccggt acccaattcg ccctatagtg agtcgtatta cgcgcgctca ctggccgtcg
     2101 ttttacaacg tcgtgactgg gaaaaccctg gcgttaccca acttaatcgc cttgcagcac
     2161 atcccccttt cgccagctgg cgtaatagcg aagaggcccg caccgatcgc ccttcccaac
     2221 agttgcgcag cctgaatggc gaatggacgc gccctgtagc ggcgcattaa gcgcggcggg
     2281 tgtggtggtt acgcgcagcg tgaccgctac acttgccagc gccctagcgc ccgctccttt
     2341 cgctttcttc ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg
     2401 ggggctccct ttagggttcc gatttagtgc tttacggcac ctcgacccca aaaaacttga
     2461 ttagggtgat ggttcacgta gtgggccatc gccctgatag acggtttttc gccctttgac
     2521 gttggagtcc acgttcttta atagtggact cttgttccaa actggaacaa cactcaaccc
     2581 tatctcggtc tattcttttg atttataagg gattttgccg atttcggcct attggttaaa
     2641 aaatgagctg atttaacaaa aatttaacgc gaattttaac aaaatattaa cgcttacaat
     2701 ttcctgatgc ggtattttct ccttacgcat ctgtgcggta tttcacaccg catagacact
     2761 ggatggcggc gttagtatcg aatcgacagc agtatagcga ccagcattca catacgattg
     2821 acgcatgata ttactttctg cgcacttaac ttcgcatctg ggcagatgat gtcgaggcga
     2881 aaaaaaatat aaatcacgct aacatttgat taaaatagaa caactacaat ataaaaaaac
     2941 tatacaaatg acaagttctt gaaaacaaga atctttttat tgtcagtact gattagaaaa
     3001 actcatcgag catcaaatga aactgcaatt tattcatatc aggattatca ataccatatt
     3061 tttgaaaaag ccgtttctgt aatgaaggag aaaactcacc gaggcagttc cataggatgg
     3121 caagatcctg gtatcggtct gcgattccga ctcgtccaac atcaatacaa cctattaatt
     3181 tcccctcgtc aaaaataagg ttatcaagtg agaaatcacc atgagtgacg actgaatccg
     3241 gtgagaatgg caaaagctta tgcatttctt tccagacttg ttcaacaggc cagccattac
     3301 gctcgtcatc aaaatcactc gcatcaacca aaccgttatt cattcgtgat tgcgcctgag
     3361 cgagacgaaa tacgcgatcg ctgttaaaag gacaattaca aacaggaatc gaatgcaacc
     3421 ggcgcaggaa cactgccagc gcatcaacaa tattttcacc tgaatcagga tattcttcta
     3481 atacctggaa tgctgttttg ccggggatcg cagtggtgag taaccatgca tcatcaggag
     3541 tacggataaa atgcttgatg gtcggaagag gcataaattc cgtcagccag tttagtctga
     3601 ccatctcatc tgtaacatca ttggcaacgc tacctttgcc atgtttcaga aacaactctg
     3661 gcgcatcggg cttcccatac aatcgataga ttgtcgcacc tgattgcccg acattatcgc
     3721 gagcccattt atacccatat aaatcagcat ccatgttgga atttaatcgc ggcctcgaaa
     3781 cgtgagtctt ttccttaccc atggttgttt atgttcggat gtgatgtgag aactgtatcc
     3841 tagcaagatt ttaaaaggaa gtatatgaaa gaagaacctc agtggcaaat cctaaccttt
     3901 tatatttctc tacaggggcg cggcgtgggg acaattcaac gcgtctgtga ggggagcgtt
     3961 tccctgctcg caggtctgca gcgaggagcc gtaatttttg cttcgcgccg tgcggccatc
     4021 aaaatgtatg gatgcaaatg attatacatg gggatgtatg ggctaaatgt acgggcgaca
     4081 gtcacatcat gcccctgagc tgcgcacgtc aagactgtca aggagggtat tctgggcctc
     4141 catgtcgctg gccgggtgac ccggcgggga cgaggcaagc taaggtggta tggtgcactc
     4201 tcagtacaat ctgctctgat gccgcatagt taagccagcc ccgacacccg ccaacacccg
     4261 ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg
     4321 tctccgggag ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc gcgagacgaa
     4381 agggcctcgt gatacgccta tttttatagg ttaatgtcat gataataatg gtttcttaga
     4441 cggatcgctt gcctgtaact tacacgcgcc tcgtatcttt taatgatgga ataatttggg
     4501 aatttactct gtgtttattt atttttatgt tttgtatttg gattttagaa agtaaataaa
     4561 gaaggtagaa gagttacgga atgaagaaaa aaaataaaca aaggtttaaa aaatttcaac
     4621 aaaaagcgta ctttacatat atatttatta gacaagaaaa gcagattaaa tagatataca
     4681 ttcgattaac gataagtaaa atgtaaaatc acaggatttt cgtgtgtggt cttctacaca
     4741 gacaagatga aacaattcgg cattaatacc tgagagcagg aagagcaaga taaaaggtag
     4801 tatttgttgg cgatccccct agagtctttt acatcttcgg aaaacaaaaa ctattttttc
     4861 tttaatttct ttttttactt tctattttta atttatatat ttatattaaa aaatttaaat
     4921 tataattatt tttatagcac gtgatgaaaa ggacccaggt ggcacttttc ggggaaatgt
     4981 gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag
     5041 acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca
     5101 tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc
     5161 agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tggg
//
