LOCUS       Exported                6267 bp ds-DNA     circular SYN 29-JUN-2022
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6267)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Wednesday, Nov 16, 2022 from SnapGene 5.0.8
            https://www.snapgene.com
COMMENT     Annotated with pLannotate v1.2.0
FEATURES             Location/Qualifiers
     source          1..6267
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     terminator      881..1078
                     /locus_tag="TEF terminator"
                     /label=TEF terminator
                     /note="pLannotate"
                     /note="/ApEinfo_hidden="
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=terminator"
     misc_feature    1114..1165
                     /locus_tag="bom (fragment)"
                     /label=bom (fragment)
                     /note="pLannotate"
                     /note="/ApEinfo_hidden="
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=36.9"
                     /note="/other=misc_feature"
     rep_origin      complement(1166..1621)
                     /direction=LEFT
                     /locus_tag="f1 ori"
                     /label=f1 ori
                     /note="pLannotate"
                     /note="/ApEinfo_hidden="
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=99.6"
                     /note="/match_length=100.0"
                     /note="/other=rep_origin"
     CDS             complement(1625..1780)
                     /locus_tag="lacZ-alpha (fragment)"
                     /label=lacZ-alpha (fragment)
                     /note="pLannotate"
                     /note="/ApEinfo_hidden="
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=89.7"
                     /note="/other=CDS"
     promoter        1788..1806
                     /locus_tag="T7 promoter"
                     /label=T7 promoter
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=promoter"
     misc_feature    complement(1815..1922)
                     /locus_tag="MCS"
                     /label=MCS
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=misc_feature"
     promoter        complement(1935..1953)
                     /locus_tag="T3 promoter"
                     /label=T3 promoter
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=promoter"
     protein_bind    complement(1998..2014)
                     /locus_tag="lac operator"
                     /label=lac operator
                     /note="pLannotate"
                     /note="/ApEinfo_hidden="
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=protein_bind"
     promoter        complement(2022..2052)
                     /locus_tag="lac promoter"
                     /label=lac promoter
                     /note="pLannotate"
                     /note="/ApEinfo_hidden="
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=promoter"
     protein_bind    complement(2067..2088)
                     /locus_tag="CAP binding site"
                     /label=CAP binding site
                     /note="pLannotate"
                     /note="/ApEinfo_hidden="
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=protein_bind"
     CDS             complement(2101..2193)
                     /locus_tag="lacI (fragment)"
                     /label=lacI (fragment)
                     /note="pLannotate"
                     /note="/ApEinfo_hidden="
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=8.6"
                     /note="/other=CDS"
     rep_origin      complement(2376..2964)
                     /direction=LEFT
                     /locus_tag="ori"
                     /label=ori
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=99.8"
                     /note="/match_length=100.0"
                     /note="/other=rep_origin"
     CDS             complement(3135..3995)
                     /locus_tag="AmpR"
                     /label=AmpR
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=99.8"
                     /note="/match_length=100.0"
                     /note="/other=CDS"
     promoter        complement(3996..4100)
                     /locus_tag="AmpR promoter"
                     /label=AmpR promoter
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=promoter"
     rep_origin      complement(4127..5473)
                     /direction=LEFT
                     /locus_tag="2u ori"
                     /label=2u ori
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=99.8"
                     /note="/match_length=100.0"
                     /note="/other=rep_origin"
     CDS             5541..5576
                     /locus_tag="rop (fragment)"
                     /label=rop (fragment)
                     /note="pLannotate"
                     /note="/ApEinfo_hidden="
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=18.8"
                     /note="/other=CDS"
     misc_feature    5678..5727
                     /locus_tag="bom (fragment)(1)"
                     /label=bom (fragment)
                     /label=bom (fragment)(1)
                     /note="pLannotate"
                     /note="/ApEinfo_hidden="
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=35.5"
                     /note="/other=misc_feature"
     gene            join(5770..6267,1..1078)
                     /locus_tag="hphMX6"
                     /label=hphMX6
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=99.9"
                     /note="/match_length=100.0"
                     /note="/other=gene"
ORIGIN
        1 atagctgcgc cgatggtttc tacaaagatc gttatgttta tcggcacttt gcatcggccg
       61 cgctcccgat tccggaagtg cttgacattg gggaattcag cgagagcctg acctattgca
      121 tctcccgccg tgcacagggt gtcacgttgc aagacctgcc tgaaaccgaa ctgcccgctg
      181 ttctgcagcc ggtcgcggag gccatggatg cgatcgctgc ggccgatctt agccagacga
      241 gcgggttcgg cccattcgga ccgcaaggaa tcggtcaata cactacatgg cgtgatttca
      301 tatgcgcgat tgctgatccc catgtgtatc actggcaaac tgtgatggac gacaccgtca
      361 gtgcgtccgt cgcgcaggct ctcgatgagc tgatgctttg ggccgaggac tgccccgaag
      421 tccggcacct cgtgcacgcg gatttcggct ccaacaatgt cctgacggac aatggccgca
      481 taacagcggt cattgactgg agcgaggcga tgttcgggga ttcccaatac gaggtcgcca
      541 acatcttctt ctggaggccg tggttggctt gtatggagca gcagacgcgc tacttcgagc
      601 ggaggcatcc ggagcttgca ggatcgccgc ggctccgggc gtatatgctc cgcattggtc
      661 ttgaccaact ctatcagagc ttggttgacg gcaatttcga tgatgcagct tgggcgcagg
      721 gtcgatgcga cgcaatcgtc cgatccggag ccgggactgt cgggcgtaca caaatcgccc
      781 gcagaagcgc ggccgtctgg accgatggct gtgtagaagt actcgccgat agtggaaacc
      841 gacgccccag cactcgtccg agggcaaagg aataatcagt actgacaata aaaagattct
      901 tgttttcaag aacttgtcat ttgtatagtt tttttatatt gtagttgttc tattttaatc
      961 aaatgttagc gtgatttata ttttttttcg cctcgacatc atctgcccag atgcgaagtt
     1021 aagtgcgcag aaagtaatat catgcgtcaa tcgtatgtga atgctggtcg ctatactgct
     1081 gtcgattcga tactaacgcc gccatccagt gtctatgcgg tgtgaaatac cgcacagatg
     1141 cgtaaggaga aaataccgca tcaggaaatt gtaagcgtta atattttgtt aaaattcgcg
     1201 ttaaattttt gttaaatcag ctcatttttt aaccaatagg ccgaaatcgg caaaatccct
     1261 tataaatcga aagaatagac cgagataggg ttgagtgttg ttccagtttg gaacaagagt
     1321 ccactattaa agaacgtgga ctccaacgtc aaagggcgaa aaaccgtcta tcagggcgat
     1381 ggcccactac gtgaaccatc accctaatca agttttttgg ggtcgaggtg ccgtaaagca
     1441 ctaaatcgga accctaaagg gagcccccga tttagagctt gacggggaaa gccggcgaac
     1501 gtggcgagaa aggaagggaa gaaagcgaaa ggagcgggcg ctagggcgct ggcaagtgta
     1561 gcggtcacgc tgcgcgtaac caccacaccc gccgcgctta atgcgccgct acagggcgcg
     1621 tccattcgcc attcaggctg cgcaactgtt gggaagggcg atcggtgcgg gcctcttcgc
     1681 tattacgcca gctggcgaaa gggggatgtg ctgcaaggcg attaagttgg gtaacgccag
     1741 ggttttccca gtcacgacgt tgtaaaacga cggccagtga gcgcgcgtaa tacgactcac
     1801 tatagggcga attgggtacc gggccccccc tcgaggtcga cggtatcgat aagcttgata
     1861 tcgaattcct gcagcccggg ggatccacta gttctagagc ggccgccacc gcggtggagc
     1921 tccagctttt gttcccttta gtgagggtta attgcgcgct tggcgtaatc atggtcatag
     1981 ctgtttcctg tgtgaaattg ttatccgctc acaattccac acaacatacg agccggaagc
     2041 ataaagtgta aagcctgggg tgcctaatga gtgagctaac tcacattaat tgcgttgcgc
     2101 tcactgcccg ctttccagtc gggaaacctg tcgtgccagc tgcattaatg aatcggccaa
     2161 cgcgcgggga gaggcggttt gcgtattggg cgctcttccg cttcctcgct cactgactcg
     2221 ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg
     2281 ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcgaaag
     2341 gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac
     2401 gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga
     2461 taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt
     2521 accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc
     2581 tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc
     2641 cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta
     2701 agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat
     2761 gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac tagaagaaca
     2821 gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct
     2881 tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt
     2941 acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct
     3001 cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc
     3061 acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa
     3121 acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta
     3181 tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc
     3241 ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat
     3301 ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta
     3361 tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt
     3421 aatagtttgc gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt
     3481 ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg
     3541 ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc
     3601 gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc
     3661 gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg
     3721 cggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga
     3781 actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta
     3841 ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct
     3901 tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag
     3961 ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca atattattga
     4021 agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat
     4081 aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctgaacg aagcatctgt
     4141 gcttcatttt gtagaacaaa aatgcaacgc gagagcgcta atttttcaaa caaagaatct
     4201 gagctgcatt tttacagaac agaaatgcaa cgcgaaagcg ctattttacc aacgaagaat
     4261 ctgtgcttca tttttgtaaa acaaaaatgc aacgcgagag cgctaatttt tcaaacaaag
     4321 aatctgagct gcatttttac agaacagaaa tgcaacgcga gagcgctatt ttaccaacaa
     4381 agaatctata cttctttttt gttctacaaa aatgcatccc gagagcgcta tttttctaac
     4441 aaagcatctt agattacttt ttttctcctt tgtgcgctct ataatgcagt ctcttgataa
     4501 ctttttgcac tgtaggtccg ttaaggttag gagaaggcta ctttggtgtc tattttctct
     4561 tccataaaaa aagcctgact ccacttcccg cgtttactga ttactagcga agctgcgggt
     4621 gcattttttc aagataaagg catccccgat tatattctat accgatgtgg attgcgcata
     4681 ctttgtgaac agaaggtgat agcgttgatg attcttcatt ggtcagaaaa ttatgaacgg
     4741 tttcttctat tttgtctcta tatactacgt ataggaaatg tttacatttt cgtattgttt
     4801 tcgattcact ctatgaatag ttcttactac aatttttttg tctaaagagt aatactagag
     4861 ataaacataa aaaatgtaga ggtcgagttt agatgcaagt tcaaggagcg aaaggtggat
     4921 gggtaggtta tatagggata tagcacagag atatatagca aagagatact tttgagcaat
     4981 gtttgtggaa gcggtattcg caatatttta gtagctcgtt acagtccggt gcgtttttgg
     5041 ttttttgaaa gtgcgtcttc agagcgcttt tggttttcaa aagcgctctg aagttcctat
     5101 actttctagc tagagaatag gaacttcgga ataggaactt caaagcgttt ccgaaaacga
     5161 gcgcttccga aaatgcaacg cgagctgcgc acatgcagct cactgttcac gtcgcaccta
     5221 tatctgcgtg ttgcctgtat atatatatac atgagaagaa cggcatagtg cgtgtttatg
     5281 cttaaatgcg tacttatatg cgtctattta tgtaggatga aaggtagtct agtacctcct
     5341 gtgatattat cccattccat gcggggtatc gtatgcttcc ttcagcacta ccctttagct
     5401 gttctatatg ctgccactcc tcaattggat tagtctcatc cttcaatgct atcatttcct
     5461 ttgatattgg atcatactaa gaaaccatta ttatcatgac attaacctat aaaaataggc
     5521 gtatcacgag gccctttcgt ctcgcgcgtt tcggtgatga cggtgaaaac ctctgacgca
     5581 tgcagctccc ggagacggtc acagcttgtc tgtaagcgga tgccgggagc agacaagccc
     5641 gtcagggcgc gtcagcgggt gttggcgggt gtcggggctg gcttaactat gcggcatcag
     5701 agcagattgt actgagagtg caccatacca ccttagcttg cctcgtcccc gccgggtcac
     5761 ccggccagcg acatggaggc ccagaatacc ctccttgaca gtcttgacgt gcgcagctca
     5821 ggggcatgat gtgactgtcg cccgtacatt tagcccatac atccccatgt ataatcattt
     5881 gcatccatac attttgatgg ccgcacggcg cgaagcaaaa attacggctc ctcgctgcgg
     5941 acctgcgagc agggaaacgc tcccctcaca gacgcgttga attgtcccca cgccgcgccc
     6001 ctgtagagaa atataaaagg ttaggatttg ccactgaggt tcttctttca tatacttcct
     6061 tttaaaatct tgctaggata cagttctcac atcacatccg aacataaaca accatgggta
     6121 aaaagcctga actcaccgcg acgtctgtcg agaagtttct gatcgaaaag ttcgacagcg
     6181 tctccgacct gatgcagctc tcggagggcg aagaatctcg tgctttcagc ttcgatgtag
     6241 gagggcgtgg atatgtcctg cgggtaa
//
