LOCUS       Exported                6927 bp ds-DNA     circular SYN 29-JUN-2022
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6927)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Wednesday, Nov 16, 2022 from SnapGene 5.0.8
            https://www.snapgene.com
COMMENT     Annotated with pLannotate v1.2.0
FEATURES             Location/Qualifiers
     source          1..6927
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     terminator      683..880
                     /locus_tag="TEF terminator"
                     /label=TEF terminator
                     /note="pLannotate"
                     /note="/ApEinfo_hidden="
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=terminator"
     misc_feature    916..967
                     /locus_tag="bom (fragment)"
                     /label=bom (fragment)
                     /note="pLannotate"
                     /note="/ApEinfo_hidden="
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=36.9"
                     /note="/other=misc_feature"
     rep_origin      complement(995..1423)
                     /direction=LEFT
                     /locus_tag="f1 ori"
                     /label=f1 ori
                     /note="pLannotate"
                     /note="/ApEinfo_hidden="
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=rep_origin"
     CDS             complement(1427..1582)
                     /locus_tag="lacZ-alpha (fragment)"
                     /label=lacZ-alpha (fragment)
                     /note="pLannotate"
                     /note="/ApEinfo_hidden="
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=89.7"
                     /note="/other=CDS"
     promoter        1590..1608
                     /locus_tag="T7 promoter"
                     /label=T7 promoter
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=promoter"
     terminator      complement(1627..1874)
                     /locus_tag="CYC1 terminator"
                     /label=CYC1 terminator
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=terminator"
     misc_feature    complement(1875..1943)
                     /locus_tag="MCS"
                     /label=MCS
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=63.9"
                     /note="/other=misc_feature"
     promoter        complement(1948..2591)
                     /locus_tag="TDH3 promoter"
                     /label=TDH3 promoter
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=99.7"
                     /note="/match_length=100.2"
                     /note="/other=promoter"
     promoter        complement(2616..2634)
                     /locus_tag="T3 promoter"
                     /label=T3 promoter
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=promoter"
     protein_bind    complement(2679..2695)
                     /locus_tag="lac operator"
                     /label=lac operator
                     /note="pLannotate"
                     /note="/ApEinfo_hidden="
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=protein_bind"
     promoter        complement(2703..2733)
                     /locus_tag="lac promoter"
                     /label=lac promoter
                     /note="pLannotate"
                     /note="/ApEinfo_hidden="
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=promoter"
     protein_bind    complement(2748..2769)
                     /locus_tag="CAP binding site"
                     /label=CAP binding site
                     /note="pLannotate"
                     /note="/ApEinfo_hidden="
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=protein_bind"
     CDS             complement(2782..2874)
                     /locus_tag="lacI (fragment)"
                     /label=lacI (fragment)
                     /note="pLannotate"
                     /note="/ApEinfo_hidden="
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=8.6"
                     /note="/other=CDS"
     rep_origin      complement(3057..3645)
                     /direction=LEFT
                     /locus_tag="ori"
                     /label=ori
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=99.7"
                     /note="/match_length=100.0"
                     /note="/other=rep_origin"
     CDS             complement(3816..4676)
                     /locus_tag="AmpR"
                     /label=AmpR
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=99.5"
                     /note="/match_length=100.0"
                     /note="/other=CDS"
     promoter        complement(4677..4781)
                     /locus_tag="AmpR promoter"
                     /label=AmpR promoter
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=promoter"
     rep_origin      complement(4808..5790)
                     /direction=LEFT
                     /locus_tag="2u ori"
                     /label=2u ori
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=rep_origin"
     protein_bind    complement(5793..5822)
                     /locus_tag="FRT (mutant) (fragment)"
                     /label=FRT (mutant) (fragment)
                     /note="pLannotate"
                     /note="/ApEinfo_hidden="
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=62.5"
                     /note="/other=protein_bind"
     CDS             complement(5975..6157)
                     /locus_tag="FLP (fragment)"
                     /label=FLP (fragment)
                     /note="pLannotate"
                     /note="/ApEinfo_hidden="
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=14.4"
                     /note="/other=CDS"
     CDS             6222..6257
                     /locus_tag="rop (fragment)"
                     /label=rop (fragment)
                     /note="pLannotate"
                     /note="/ApEinfo_hidden="
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=18.8"
                     /note="/other=CDS"
     misc_feature    6359..6408
                     /locus_tag="bom (fragment)(1)"
                     /label=bom (fragment)
                     /label=bom (fragment)(1)
                     /note="pLannotate"
                     /note="/ApEinfo_hidden="
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=35.5"
                     /note="/other=misc_feature"
     promoter        6451..6794
                     /locus_tag="TEF promoter"
                     /label=TEF promoter
                     /note="pLannotate"
                     /note="/ApEinfo_hidden="
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=promoter"
     CDS             join(6795..6927,1..677)
                     /locus_tag="kanMX"
                     /label=kanMX
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=99.5"
                     /note="/match_length=100.0"
                     /note="/other=CDS"
ORIGIN
        1 ccgatgcgcc agagttgttt ctgaaacatg gcaaaggtag cgttgccaat gatgttacag
       61 atgagatggt cagactaaac tggctgacgg aatttatgcc tcttccgacc atcaagcatt
      121 ttatccgtac tcctgatgat gcatggttac tcaccactgc gatccccggc aaaacagcat
      181 tccaggtatt agaagaatat cctgattcag gtgaaaatat tgttgatgcg ctggcagtgt
      241 tcctgcgccg gttgcattcg attcctattt gtaattgtcc ttttaacagc gatcgcgtat
      301 ttcgtctcgc tcaggcgcaa tcacgaatga ataacggttt ggttgatgcg agtgattttg
      361 atgacgagcg taatggctgg cctgttgaac aagtctggaa agaaatgcat aagcttttgc
      421 cattctcacc ggattcagtc gtcactcatg gtgatttctc acttgataac cttatttttg
      481 acgaggggaa attaataggt tgtattgatg ttggacgagt cggaatcgca gaccgatacc
      541 aggatcttgc catcctatgg aactgcctcg gtgatttttt tccttcatta cagaaacggc
      601 tttttcaaaa atatggtatt gataatcctg atatgaataa attgcagttt catttgatgc
      661 ttgatgagtt tttctaatca gtactgacaa taaaaagatt cttgttttca agaacttgtc
      721 atttgtatag tttttttata ttgtagttgt tctattttaa tcaaatgtta gcgtgattta
      781 tatttttttt cgcctcgaca tcatctgccc agatgcgaag ttaagtgcgc agaaagtaat
      841 atcatgcgtc aatcgtatgt gaatgctggt cgctatactg ctgtcgattc gatactaacg
      901 ccgccatcca gtgtctatgc ggtgtgaaat accgcacaga tgcgtaagga gaaaataccg
      961 catcaggaaa ttgtaagcgt taatattttg ttaaaattcg cgttaaattt ttgttaaatc
     1021 agctcatttt ttaaccaata ggccgaaatc ggcaaaatcc cttataaatc aaaagaatag
     1081 accgagatag ggttgagtgt tgttccagtt tggaacaaga gtccactatt aaagaacgtg
     1141 gactccaacg tcaaagggcg aaaaaccgtc tatcagggcg atggcccact acgtgaacca
     1201 tcaccctaat caagtttttt ggggtcgagg tgccgtaaag cactaaatcg gaaccctaaa
     1261 gggagccccc gatttagagc ttgacgggga aagccggcga acgtggcgag aaaggaaggg
     1321 aagaaagcga aaggagcggg cgctagggcg ctggcaagtg tagcggtcac gctgcgcgta
     1381 accaccacac ccgccgcgct taatgcgccg ctacagggcg cgtccattcg ccattcaggc
     1441 tgcgcaactg ttgggaaggg cgatcggtgc gggcctcttc gctattacgc cagctggcga
     1501 aagggggatg tgctgcaagg cgattaagtt gggtaacgcc agggttttcc cagtcacgac
     1561 gttgtaaaac gacggccagt gagcgcgcgt aatacgactc actatagggc gaattgggta
     1621 ccggccgcaa attaaagcct tcgagcgtcc caaaaccttc tcaagcaagg ttttcagtat
     1681 aatgttacat gcgtacacgc gtctgtacag aaaaaaaaga aaaatttgaa atataaataa
     1741 cgttcttaat actaacataa ctataaaaaa ataaataggg acctagactt caggttgtct
     1801 aactccttcc ttttcggtta gagcggatgt ggggggaggg cgtgaatgta agcgtgacat
     1861 aactaattac atgactcgag gtcgacggta tcgataagct tgatatcgaa ttcctgcagc
     1921 ccgggggatc cactagttct agatccgtcg aaactaagtt cttggtgttt taaaactaaa
     1981 aaaaagacta actataaaag tagaatttaa gaagtttaag aaatagattt acagaattac
     2041 aatcaatacc taccgtcttt atatacttat tagtcaagta ggggaataat ttcagggaac
     2101 tggtttcaac cttttttttc agctttttcc aaatcagaga gagcagaagg taatagaagg
     2161 tgtaagaaaa tgagatagat acatgcgtgg gtcaattgcc ttgtgtcatc atttactcca
     2221 ggcaggttgc atcactccat tgaggttgtg cccgtttttt gcctgtttgt gcccctgttc
     2281 tctgtagttg cgctaagaga atggacctat gaactgatgg ttggtgaaga aaacaatatt
     2341 ttggtgctgg gattcttttt tttctggatg ccagcttaaa aagcgggctc cattatattt
     2401 agtggatgcc aggaataaac tgttcaccca gacacctacg atgttatata ttctgtgtaa
     2461 cccgccccct attttgggca tgtacgggtt acagcagaat taaaaggcta attttttgac
     2521 taaataaagt taggaaaatc actactatta attatttacg tattctttga aatggcgagt
     2581 attgataatg ataaactgag ctccagcttt tgttcccttt agtgagggtt aattgcgcgc
     2641 ttggcgtaat catggtcata gctgtttcct gtgtgaaatt gttatccgct cacaattcca
     2701 cacaacatac gagccggaag cataaagtgt aaagcctggg gtgcctaatg agtgagctaa
     2761 ctcacattaa ttgcgttgcg ctcactgccc gctttccagt cgggaaacct gtcgtgccag
     2821 ctgcattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc
     2881 gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct
     2941 cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg
     3001 tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc
     3061 cataggctcc gcccccctga cgagcatcac aaaaatggac gctcaagtca gaggtggcga
     3121 aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct
     3181 cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg
     3241 gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag
     3301 ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat
     3361 cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac
     3421 aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac
     3481 tacggctaca ctagaagaac agtatttggt atctgcgctc tgctgaagcc agttaccttc
     3541 ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt
     3601 tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc
     3661 ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg
     3721 agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca
     3781 atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca
     3841 cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag
     3901 ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac
     3961 ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc
     4021 agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct
     4081 agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc
     4141 gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggctccca acgatcaagg
     4201 cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc
     4261 gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat
     4321 tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag
     4381 tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat
     4441 aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg
     4501 cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca
     4561 cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga
     4621 aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aacgttgaat actcatactc
     4681 ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag cggatacata
     4741 tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg
     4801 ccacctgaac gaagcatctg tgcttcattt tgtagaacaa aaatgcaacg cgagagcgct
     4861 aatttttcaa acaaagaatc tgagctgcat ttttacagaa cagaaatgca acgcgaaagc
     4921 gctattttac caacgaagaa tctgtgcttc atttttgtaa aacaaaaatg caacgcgaga
     4981 gcgctaattt ttcaaacaaa gaatctgagc tgcattttta cagaacagaa atgcaacgcg
     5041 agagcgctat tttaccaaca aagaatctat acttcttttt tgttctacaa aaatgcatcc
     5101 cgagagcgct atttttctaa caaagcatct tagattactt tttttctcct ttgtgcgctc
     5161 tataatgcag tctcttgata actttttgca ctgtaggtcc gttaaggtta gaagaaggct
     5221 actttggtgt ctattttctc ttccataaaa aaagcctgac tccacttccc gcgtttactg
     5281 attactagcg aagctgcggg tgcatttttt caagataaag gcatccccga ttatattcta
     5341 taccgatgtg gattgcgcat actttgtgaa cagaaagtga tagcgttgat gattcttcat
     5401 tggtcagaaa attatgaacg gtttcttcta ttttgtctct atatactacg tataggaaat
     5461 gtttacattt tcgtattgtt ttcgattcac tctatgaata gttcttacta caattttttt
     5521 gtctaaagag taatactaga gataaacata aaaaatgtag aggtcgagtt tagatgcaag
     5581 ttcaaggagc gaaaggtgga tgggtaggtt atatagggat atagcacaga gatatatagc
     5641 aaagagatac ttttgagcaa tgtttgtgga agcggtattc gcaatatttt agtagctcgt
     5701 tacagtccgg tgcgtttttg gttttttgaa agtgcgtctt cagagcgctt ttggttttca
     5761 aaagcgctct gaagttccta tactttctag ctagagaata ggaacttcgg aataggaact
     5821 tcaaagcgtt tccgaaaacg agcgcttccg aaaatgcaac gcgagctgcg cacatacagc
     5881 tcactgttca cgtcgcacct atatctgcgt gttgcctgta tatatatata cgtgagaaga
     5941 acggcatagt gcgtgtttat gcttaaatgc gtacttatat gcgtctattt atgtaggatg
     6001 aaaggtagtc tagtacctcc tgtgatatta tcccattcca tgcggggtat cgtatgcttc
     6061 cttcagcact accctttagc tgttctatat gctgccactc ctcaattgga ttagtctcat
     6121 ccttcaatgc tatcatttcc tttgatattg gatcatacta agaaaccatt attatcatga
     6181 cattaaccta taaaaatagg cgtatcacga ggccctttcg tctcgcgcgt ttcggtgatg
     6241 acggtgaaaa cctctgacac atgcagctcc cggagacggt cacagcttgt ctgtaagcgg
     6301 atgccgggag cagacaagcc cgtcagggcg cgtcagcggg tgttggcggg tgtcggggct
     6361 ggcttaacta tgcggcatca gagcagattg tactgagagt gcaccatacc accttagctt
     6421 gcctcgtccc cgccgggtca cccggccagc gacatggagg cccagaatac cctccttgac
     6481 agtcttgacg tgcgcagctc aggggcatga tgtgactgtc gcccgtacat ttagcccata
     6541 catccccatg tataatcatt tgcatccata cattttgatg gccgcacggc gcgaagcaaa
     6601 aattacggct cctcgctgca gacctgcgag cagggaaacg ctcccctcac agacgcgttg
     6661 aattgtcccc acgccgcgcc cctgtagaga aatataaaag gttaggattt gccactgagg
     6721 ttcttctttc atatacttcc ttttaaaatc ttgctaggat acagttctca catcacatcc
     6781 gaacataaac aaccatgggt aaggaaaaga ctcacgtttc gaggccgcga ttaaattcca
     6841 acatggatgc tgatttatat gggtataaat gggctcgcga taatgtcggg caatcaggtg
     6901 cgacaatcta tcgattgtat gggaagc
//
