LOCUS       Exported                6694 bp ds-DNA     circular SYN 29-JUN-2022
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6694)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Wednesday, Nov 16, 2022 from SnapGene 5.0.8
            https://www.snapgene.com
COMMENT     Annotated with pLannotate v1.2.0
FEATURES             Location/Qualifiers
     source          1..6694
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    109..158
                     /locus_tag="bom (fragment)(1)"
                     /label=bom (fragment)
                     /label=bom (fragment)(1)
                     /note="pLannotate"
                     /note="/ApEinfo_hidden="
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=35.5"
                     /note="/other=misc_feature"
     promoter        201..544
                     /locus_tag="TEF promoter"
                     /label=TEF promoter
                     /note="pLannotate"
                     /note="/ApEinfo_hidden="
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=promoter"
     CDS             551..1114
                     /locus_tag="natAC"
                     /label=natAC
                     /note="pLannotate"
                     /note="/database=swissprot"
                     /note="/fragment=False"
                     /note="/identity=72.9"
                     /note="/match_length=99.5"
                     /note="/other=CDS"
     terminator      1126..1323
                     /locus_tag="TEF terminator"
                     /label=TEF terminator
                     /note="pLannotate"
                     /note="/ApEinfo_hidden="
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=terminator"
     misc_feature    1359..1410
                     /locus_tag="bom (fragment)"
                     /label=bom (fragment)
                     /note="pLannotate"
                     /note="/ApEinfo_hidden="
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=36.9"
                     /note="/other=misc_feature"
     rep_origin      complement(1411..1866)
                     /direction=LEFT
                     /locus_tag="f1 ori"
                     /label=f1 ori
                     /note="pLannotate"
                     /note="/ApEinfo_hidden="
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=99.6"
                     /note="/match_length=100.0"
                     /note="/other=rep_origin"
     CDS             complement(1870..2025)
                     /locus_tag="lacZ-alpha (fragment)"
                     /label=lacZ-alpha (fragment)
                     /note="pLannotate"
                     /note="/ApEinfo_hidden="
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=89.7"
                     /note="/other=CDS"
     promoter        2033..2051
                     /locus_tag="T7 promoter"
                     /label=T7 promoter
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=promoter"
     terminator      complement(2082..2271)
                     /locus_tag="CYC1 terminator"
                     /label=CYC1 terminator
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=terminator"
     misc_feature    complement(2319..2387)
                     /locus_tag="MCS"
                     /label=MCS
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=63.9"
                     /note="/other=misc_feature"
     promoter        complement(2392..3035)
                     /locus_tag="TDH3 promoter"
                     /label=TDH3 promoter
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=99.7"
                     /note="/match_length=100.2"
                     /note="/other=promoter"
     promoter        complement(3060..3078)
                     /locus_tag="T3 promoter"
                     /label=T3 promoter
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=promoter"
     protein_bind    complement(3123..3139)
                     /locus_tag="lac operator"
                     /label=lac operator
                     /note="pLannotate"
                     /note="/ApEinfo_hidden="
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=protein_bind"
     promoter        complement(3147..3177)
                     /locus_tag="lac promoter"
                     /label=lac promoter
                     /note="pLannotate"
                     /note="/ApEinfo_hidden="
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=promoter"
     protein_bind    complement(3192..3213)
                     /locus_tag="CAP binding site"
                     /label=CAP binding site
                     /note="pLannotate"
                     /note="/ApEinfo_hidden="
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=protein_bind"
     CDS             complement(3226..3318)
                     /locus_tag="lacI (fragment)"
                     /label=lacI (fragment)
                     /note="pLannotate"
                     /note="/ApEinfo_hidden="
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=8.6"
                     /note="/other=CDS"
     rep_origin      complement(3501..4089)
                     /direction=LEFT
                     /locus_tag="ori"
                     /label=ori
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=99.8"
                     /note="/match_length=100.0"
                     /note="/other=rep_origin"
     CDS             complement(4260..5120)
                     /locus_tag="AmpR"
                     /label=AmpR
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=99.8"
                     /note="/match_length=100.0"
                     /note="/other=CDS"
     promoter        complement(5121..5225)
                     /locus_tag="AmpR promoter"
                     /label=AmpR promoter
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=promoter"
     rep_origin      complement(5252..6598)
                     /direction=LEFT
                     /locus_tag="2u ori"
                     /label=2u ori
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=99.8"
                     /note="/match_length=100.0"
                     /note="/other=rep_origin"
     CDS             join(6666..6694,1..7)
                     /locus_tag="rop (fragment)"
                     /label=rop (fragment)
                     /note="pLannotate"
                     /note="/ApEinfo_hidden="
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=18.8"
                     /note="/other=CDS"
ORIGIN
        1 cctctgacgc atgcagctcc cggagacggt cacagcttgt ctgtaagcgg atgccgggag
       61 cagacaagcc cgtcagggcg cgtcagcggg tgttggcggg tgtcggggct ggcttaacta
      121 tgcggcatca gagcagattg tactgagagt gcaccatacc accttagctt gcctcgtccc
      181 cgccgggtca cccggccagc gacatggagg cccagaatac cctccttgac agtcttgacg
      241 tgcgcagctc aggggcatga tgtgactgtc gcccgtacat ttagcccata catccccatg
      301 tataatcatt tgcatccata cattttgatg gccgcacggc gcgaagcaaa aattacggct
      361 cctcgctgca gacctgcgag cagggaaacg ctcccctcac agacgcgttg aattgtcccc
      421 acgccgcgcc cctgtagaga aatataaaag gttaggattt gccactgagg ttcttctttc
      481 atatacttcc ttttaaaatc ttgctaggat acagttctca catcacatcc gaacataaac
      541 aaccatgggt actacattag acgacaccgc ctacaggtat cgtacatctg tcccaggtga
      601 tgcagaagct attgaagctc ttgatggttc tttcacgacg gataccgttt tccgtgttac
      661 agctacagga gacggcttta cattaagaga agttccagtc gatccacctc taaccaaagt
      721 gtttcctgat gatgaaagcg atgatgaaag tgacgatggt gaagacggag acccggatag
      781 tagaacattt gtggcctacg gcgacgacgg agatttagct ggtttcgtag tagtctcata
      841 ttcggggtgg aacagaagac ttacagtcga agacattgaa gttgctcctg aacatagggg
      901 tcacggtgtg ggtagggctc tgatgggact tgcaacagaa tttgcacgtg aaagaggtgc
      961 aggtcatttg tggttggaag tcaccaacgt aaatgcaccg gcaatccacg catacagaag
     1021 aatgggtttt actttatgtg gccttgatac agccttgtat gacggaactg cttcagatgg
     1081 agaacaggca ttatatatgt caatgccttg cccttgataa tcagtactga caataaaaag
     1141 attcttgttt tcaagaactt gtcatttgta tagttttttt atattgtagt tgttctattt
     1201 taatcaaatg ttagcgtgat ttatattttt tttcgcctcg acatcatctg cccagatgcg
     1261 aagttaagtg cgcagaaagt aatatcatgc gtcaatcgta tgtgaatgct ggtcgctata
     1321 ctgctgtcga ttcgatacta acgccgccat ccagtgtcta tgcggtgtga aataccgcac
     1381 agatgcgtaa ggagaaaata ccgcatcagg aaattgtaag cgttaatatt ttgttaaaat
     1441 tcgcgttaaa tttttgttaa atcagctcat tttttaacca ataggccgaa atcggcaaaa
     1501 tcccttataa atcgaaagaa tagaccgaga tagggttgag tgttgttcca gtttggaaca
     1561 agagtccact attaaagaac gtggactcca acgtcaaagg gcgaaaaacc gtctatcagg
     1621 gcgatggccc actacgtgaa ccatcaccct aatcaagttt tttggggtcg aggtgccgta
     1681 aagcactaaa tcggaaccct aaagggagcc cccgatttag agcttgacgg ggaaagccgg
     1741 cgaacgtggc gagaaaggaa gggaagaaag cgaaaggagc gggcgctagg gcgctggcaa
     1801 gtgtagcggt cacgctgcgc gtaaccacca cacccgccgc gcttaatgcg ccgctacagg
     1861 gcgcgtccat tcgccattca ggctgcgcaa ctgttgggaa gggcgatcgg tgcgggcctc
     1921 ttcgctatta cgccagctgg cgaaaggggg atgtgctgca aggcgattaa gttgggtaac
     1981 gccagggttt tcccagtcac gacgttgtaa aacgacggcc agtgagcgcg cgtaatacga
     2041 ctcactatag ggcgaattgg gtaccggccg caaattaaag ccttcgagcg tcccaaaacc
     2101 ttctcaagca aggttttcag tataatgtta catgcgtaca cgcgtctgta cagaaaaaaa
     2161 agaaaaattt gaaatataaa taacgttctt aatactaaca taactataaa aaaataaata
     2221 gggacctaga cttcaggttg tctaactcct tccttttcgg ttagagcgga tgtggggggg
     2281 agggcgtgaa tgtaagcgtg acataactaa ttacatgact cgaggtcgac ggtatcgata
     2341 agcttgatat cgaattcctg cagcccgggg gatccactag ttctagatcc gtcgaaacta
     2401 agttcttggt gttttaaaac taaaaaaaag actaactata aaagtagaat ttaagaagtt
     2461 taagaaatag atttacagaa ttacaatcaa tacctaccgt ctttatatac ttattagtca
     2521 agtaggggaa taatttcagg gaactggttt caaccttttt tttcagcttt ttccaaatca
     2581 gagagagcag aaggtaatag aaggtgtaag aaaatgagat agatacatgc gtgggtcaat
     2641 tgccttgtgt catcatttac tccaggcagg ttgcatcact ccattgaggt tgtgcccgtt
     2701 ttttgcctgt ttgtgcccct gttctctgta gttgcgctaa gagaatggac ctatgaactg
     2761 atggttggtg aagaaaacaa tattttggtg ctgggattct tttttttctg gatgccagct
     2821 taaaaagcgg gctccattat atttagtgga tgccaggaat aaactgttca cccagacacc
     2881 tacgatgtta tatattctgt gtaacccgcc ccctattttg ggcatgtacg ggttacagca
     2941 gaattaaaag gctaattttt tgactaaata aagttaggaa aatcactact attaattatt
     3001 tacgtattct ttgaaatggc gagtattgat aatgataaac tgagctccag cttttgttcc
     3061 ctttagtgag ggttaattgc gcgcttggcg taatcatggt catagctgtt tcctgtgtga
     3121 aattgttatc cgctcacaat tccacacaac atacgagccg gaagcataaa gtgtaaagcc
     3181 tggggtgcct aatgagtgag ctaactcaca ttaattgcgt tgcgctcact gcccgctttc
     3241 cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc ggggagaggc
     3301 ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt
     3361 cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca
     3421 ggggataacg caggaaagaa catgtgagca aaaggccagc gaaaggccag gaaccgtaaa
     3481 aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat
     3541 cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc
     3601 cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc
     3661 gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt
     3721 tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac
     3781 cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg
     3841 ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca
     3901 gagttcttga agtggtggcc taactacggc tacactagaa gaacagtatt tggtatctgc
     3961 gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa
     4021 accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa
     4081 ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac
     4141 tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta
     4201 aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt
     4261 taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata
     4321 gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc
     4381 agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac
     4441 cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag
     4501 tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac
     4561 gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc
     4621 agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg
     4681 gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc
     4741 atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct
     4801 gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc
     4861 tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt aaaagtgctc
     4921 atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc
     4981 agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc
     5041 gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca
     5101 cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt
     5161 tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt
     5221 ccgcgcacat ttccccgaaa agtgccacct gaacgaagca tctgtgcttc attttgtaga
     5281 acaaaaatgc aacgcgagag cgctaatttt tcaaacaaag aatctgagct gcatttttac
     5341 agaacagaaa tgcaacgcga aagcgctatt ttaccaacga agaatctgtg cttcattttt
     5401 gtaaaacaaa aatgcaacgc gagagcgcta atttttcaaa caaagaatct gagctgcatt
     5461 tttacagaac agaaatgcaa cgcgagagcg ctattttacc aacaaagaat ctatacttct
     5521 tttttgttct acaaaaatgc atcccgagag cgctattttt ctaacaaagc atcttagatt
     5581 actttttttc tcctttgtgc gctctataat gcagtctctt gataactttt tgcactgtag
     5641 gtccgttaag gttaggagaa ggctactttg gtgtctattt tctcttccat aaaaaaagcc
     5701 tgactccact tcccgcgttt actgattact agcgaagctg cgggtgcatt ttttcaagat
     5761 aaaggcatcc ccgattatat tctataccga tgtggattgc gcatactttg tgaacagaag
     5821 gtgatagcgt tgatgattct tcattggtca gaaaattatg aacggtttct tctattttgt
     5881 ctctatatac tacgtatagg aaatgtttac attttcgtat tgttttcgat tcactctatg
     5941 aatagttctt actacaattt ttttgtctaa agagtaatac tagagataaa cataaaaaat
     6001 gtagaggtcg agtttagatg caagttcaag gagcgaaagg tggatgggta ggttatatag
     6061 ggatatagca cagagatata tagcaaagag atacttttga gcaatgtttg tggaagcggt
     6121 attcgcaata ttttagtagc tcgttacagt ccggtgcgtt tttggttttt tgaaagtgcg
     6181 tcttcagagc gcttttggtt ttcaaaagcg ctctgaagtt cctatacttt ctagctagag
     6241 aataggaact tcggaatagg aacttcaaag cgtttccgaa aacgagcgct tccgaaaatg
     6301 caacgcgagc tgcgcacatg cagctcactg ttcacgtcgc acctatatct gcgtgttgcc
     6361 tgtatatata tatacatgag aagaacggca tagtgcgtgt ttatgcttaa atgcgtactt
     6421 atatgcgtct atttatgtag gatgaaaggt agtctagtac ctcctgtgat attatcccat
     6481 tccatgcggg gtatcgtatg cttccttcag cactaccctt tagctgttct atatgctgcc
     6541 actcctcaat tggattagtc tcatccttca atgctatcat ttcctttgat attggatcat
     6601 actaagaaac cattattatc atgacattaa cctataaaaa taggcgtatc acgaggccct
     6661 ttcgtctcgc gcgtttcggt gatgacggtg aaaa
//
