LOCUS       Exported                7146 bp ds-DNA     circular SYN 29-JUN-2022
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7146)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Wednesday, Nov 16, 2022 from SnapGene 5.0.8
            https://www.snapgene.com
COMMENT     Annotated with pLannotate v1.2.0
FEATURES             Location/Qualifiers
     source          1..7146
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     terminator      900..1097
                     /locus_tag="TEF terminator"
                     /label=TEF terminator
                     /note="pLannotate"
                     /note="/ApEinfo_hidden="
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=terminator"
     misc_feature    1133..1184
                     /locus_tag="bom (fragment)"
                     /label=bom (fragment)
                     /note="pLannotate"
                     /note="/ApEinfo_hidden="
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=36.9"
                     /note="/other=misc_feature"
     rep_origin      complement(1185..1640)
                     /direction=LEFT
                     /locus_tag="f1 ori"
                     /label=f1 ori
                     /note="pLannotate"
                     /note="/ApEinfo_hidden="
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=99.6"
                     /note="/match_length=100.0"
                     /note="/other=rep_origin"
     CDS             complement(1644..1799)
                     /locus_tag="lacZ-alpha (fragment)"
                     /label=lacZ-alpha (fragment)
                     /note="pLannotate"
                     /note="/ApEinfo_hidden="
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=89.7"
                     /note="/other=CDS"
     promoter        1807..1825
                     /locus_tag="T7 promoter"
                     /label=T7 promoter
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=promoter"
     terminator      complement(1844..2091)
                     /locus_tag="CYC1 terminator"
                     /label=CYC1 terminator
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=terminator"
     misc_feature    complement(2092..2160)
                     /locus_tag="MCS"
                     /label=MCS
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=63.9"
                     /note="/other=misc_feature"
     promoter        complement(2165..2808)
                     /locus_tag="TDH3 promoter"
                     /label=TDH3 promoter
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=99.7"
                     /note="/match_length=100.2"
                     /note="/other=promoter"
     promoter        complement(2833..2851)
                     /locus_tag="T3 promoter"
                     /label=T3 promoter
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=promoter"
     protein_bind    complement(2896..2912)
                     /locus_tag="lac operator"
                     /label=lac operator
                     /note="pLannotate"
                     /note="/ApEinfo_hidden="
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=protein_bind"
     promoter        complement(2920..2950)
                     /locus_tag="lac promoter"
                     /label=lac promoter
                     /note="pLannotate"
                     /note="/ApEinfo_hidden="
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=promoter"
     protein_bind    complement(2965..2986)
                     /locus_tag="CAP binding site"
                     /label=CAP binding site
                     /note="pLannotate"
                     /note="/ApEinfo_hidden="
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=protein_bind"
     CDS             complement(2999..3091)
                     /locus_tag="lacI (fragment)"
                     /label=lacI (fragment)
                     /note="pLannotate"
                     /note="/ApEinfo_hidden="
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=8.6"
                     /note="/other=CDS"
     rep_origin      complement(3274..3862)
                     /direction=LEFT
                     /locus_tag="ori"
                     /label=ori
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=99.8"
                     /note="/match_length=100.0"
                     /note="/other=rep_origin"
     CDS             complement(4033..4893)
                     /locus_tag="AmpR"
                     /label=AmpR
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=99.8"
                     /note="/match_length=100.0"
                     /note="/other=CDS"
     promoter        complement(4894..4998)
                     /locus_tag="AmpR promoter"
                     /label=AmpR promoter
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=promoter"
     rep_origin      complement(5025..6371)
                     /direction=LEFT
                     /locus_tag="2u ori"
                     /label=2u ori
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=99.8"
                     /note="/match_length=100.0"
                     /note="/other=rep_origin"
     CDS             6439..6474
                     /locus_tag="rop (fragment)"
                     /label=rop (fragment)
                     /note="pLannotate"
                     /note="/ApEinfo_hidden="
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=18.8"
                     /note="/other=CDS"
     misc_feature    6576..6625
                     /locus_tag="bom (fragment)(1)"
                     /label=bom (fragment)
                     /label=bom (fragment)(1)
                     /note="pLannotate"
                     /note="/ApEinfo_hidden="
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=35.5"
                     /note="/other=misc_feature"
     gene            join(6668..7146,1..1097)
                     /locus_tag="hphMX6"
                     /label=hphMX6
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=99.9"
                     /note="/match_length=100.0"
                     /note="/other=gene"
ORIGIN
        1 ggatatgtcc tgcgggtaaa tagctgcgcc gatggtttct acaaagatcg ttatgtttat
       61 cggcactttg catcggccgc gctcccgatt ccggaagtgc ttgacattgg ggaattcagc
      121 gagagcctga cctattgcat ctcccgccgt gcacagggtg tcacgttgca agacctgcct
      181 gaaaccgaac tgcccgctgt tctgcagccg gtcgcggagg ccatggatgc gatcgctgcg
      241 gccgatctta gccagacgag cgggttcggc ccattcggac cgcaaggaat cggtcaatac
      301 actacatggc gtgatttcat atgcgcgatt gctgatcccc atgtgtatca ctggcaaact
      361 gtgatggacg acaccgtcag tgcgtccgtc gcgcaggctc tcgatgagct gatgctttgg
      421 gccgaggact gccccgaagt ccggcacctc gtgcacgcgg atttcggctc caacaatgtc
      481 ctgacggaca atggccgcat aacagcggtc attgactgga gcgaggcgat gttcggggat
      541 tcccaatacg aggtcgccaa catcttcttc tggaggccgt ggttggcttg tatggagcag
      601 cagacgcgct acttcgagcg gaggcatccg gagcttgcag gatcgccgcg gctccgggcg
      661 tatatgctcc gcattggtct tgaccaactc tatcagagct tggttgacgg caatttcgat
      721 gatgcagctt gggcgcaggg tcgatgcgac gcaatcgtcc gatccggagc cgggactgtc
      781 gggcgtacac aaatcgcccg cagaagcgcg gccgtctgga ccgatggctg tgtagaagta
      841 ctcgccgata gtggaaaccg acgccccagc actcgtccga gggcaaagga ataatcagta
      901 ctgacaataa aaagattctt gttttcaaga acttgtcatt tgtatagttt ttttatattg
      961 tagttgttct attttaatca aatgttagcg tgatttatat tttttttcgc ctcgacatca
     1021 tctgcccaga tgcgaagtta agtgcgcaga aagtaatatc atgcgtcaat cgtatgtgaa
     1081 tgctggtcgc tatactgctg tcgattcgat actaacgccg ccatccagtg tctatgcggt
     1141 gtgaaatacc gcacagatgc gtaaggagaa aataccgcat caggaaattg taagcgttaa
     1201 tattttgtta aaattcgcgt taaatttttg ttaaatcagc tcatttttta accaataggc
     1261 cgaaatcggc aaaatccctt ataaatcgaa agaatagacc gagatagggt tgagtgttgt
     1321 tccagtttgg aacaagagtc cactattaaa gaacgtggac tccaacgtca aagggcgaaa
     1381 aaccgtctat cagggcgatg gcccactacg tgaaccatca ccctaatcaa gttttttggg
     1441 gtcgaggtgc cgtaaagcac taaatcggaa ccctaaaggg agcccccgat ttagagcttg
     1501 acggggaaag ccggcgaacg tggcgagaaa ggaagggaag aaagcgaaag gagcgggcgc
     1561 tagggcgctg gcaagtgtag cggtcacgct gcgcgtaacc accacacccg ccgcgcttaa
     1621 tgcgccgcta cagggcgcgt ccattcgcca ttcaggctgc gcaactgttg ggaagggcga
     1681 tcggtgcggg cctcttcgct attacgccag ctggcgaaag ggggatgtgc tgcaaggcga
     1741 ttaagttggg taacgccagg gttttcccag tcacgacgtt gtaaaacgac ggccagtgag
     1801 cgcgcgtaat acgactcact atagggcgaa ttgggtaccg gccgcaaatt aaagccttcg
     1861 agcgtcccaa aaccttctca agcaaggttt tcagtataat gttacatgcg tacacgcgtc
     1921 tgtacagaaa aaaaagaaaa atttgaaata taaataacgt tcttaatact aacataacta
     1981 taaaaaaata aatagggacc tagacttcag gttgtctaac tccttccttt tcggttagag
     2041 cggatgtggg gggagggcgt gaatgtaagc gtgacataac taattacatg actcgaggtc
     2101 gacggtatcg ataagcttga tatcgaattc ctgcagcccg ggggatccac tagttctaga
     2161 tccgtcgaaa ctaagttctt ggtgttttaa aactaaaaaa aagactaact ataaaagtag
     2221 aatttaagaa gtttaagaaa tagatttaca gaattacaat caatacctac cgtctttata
     2281 tacttattag tcaagtaggg gaataatttc agggaactgg tttcaacctt ttttttcagc
     2341 tttttccaaa tcagagagag cagaaggtaa tagaaggtgt aagaaaatga gatagataca
     2401 tgcgtgggtc aattgccttg tgtcatcatt tactccaggc aggttgcatc actccattga
     2461 ggttgtgccc gttttttgcc tgtttgtgcc cctgttctct gtagttgcgc taagagaatg
     2521 gacctatgaa ctgatggttg gtgaagaaaa caatattttg gtgctgggat tctttttttt
     2581 ctggatgcca gcttaaaaag cgggctccat tatatttagt ggatgccagg aataaactgt
     2641 tcacccagac acctacgatg ttatatattc tgtgtaaccc gccccctatt ttgggcatgt
     2701 acgggttaca gcagaattaa aaggctaatt ttttgactaa ataaagttag gaaaatcact
     2761 actattaatt atttacgtat tctttgaaat ggcgagtatt gataatgata aactgagctc
     2821 cagcttttgt tccctttagt gagggttaat tgcgcgcttg gcgtaatcat ggtcatagct
     2881 gtttcctgtg tgaaattgtt atccgctcac aattccacac aacatacgag ccggaagcat
     2941 aaagtgtaaa gcctggggtg cctaatgagt gagctaactc acattaattg cgttgcgctc
     3001 actgcccgct ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa tcggccaacg
     3061 cgcggggaga ggcggtttgc gtattgggcg ctcttccgct tcctcgctca ctgactcgct
     3121 gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt
     3181 atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcgaaaggc
     3241 caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga
     3301 gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata
     3361 ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac
     3421 cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg
     3481 taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc
     3541 cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag
     3601 acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt
     3661 aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta gaagaacagt
     3721 atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg
     3781 atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac
     3841 gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca
     3901 gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac
     3961 ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac
     4021 ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt
     4081 tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt
     4141 accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt
     4201 atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc
     4261 cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa
     4321 tagtttgcgc aacgttgttg ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg
     4381 tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt
     4441 gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc
     4501 agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt
     4561 aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg
     4621 gcgaccgagt tgctcttgcc cggcgtcaat acgggataat accgcgccac atagcagaac
     4681 tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc
     4741 gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt
     4801 tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg
     4861 aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat attattgaag
     4921 catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa
     4981 acaaataggg gttccgcgca catttccccg aaaagtgcca cctgaacgaa gcatctgtgc
     5041 ttcattttgt agaacaaaaa tgcaacgcga gagcgctaat ttttcaaaca aagaatctga
     5101 gctgcatttt tacagaacag aaatgcaacg cgaaagcgct attttaccaa cgaagaatct
     5161 gtgcttcatt tttgtaaaac aaaaatgcaa cgcgagagcg ctaatttttc aaacaaagaa
     5221 tctgagctgc atttttacag aacagaaatg caacgcgaga gcgctatttt accaacaaag
     5281 aatctatact tcttttttgt tctacaaaaa tgcatcccga gagcgctatt tttctaacaa
     5341 agcatcttag attacttttt ttctcctttg tgcgctctat aatgcagtct cttgataact
     5401 ttttgcactg taggtccgtt aaggttagga gaaggctact ttggtgtcta ttttctcttc
     5461 cataaaaaaa gcctgactcc acttcccgcg tttactgatt actagcgaag ctgcgggtgc
     5521 attttttcaa gataaaggca tccccgatta tattctatac cgatgtggat tgcgcatact
     5581 ttgtgaacag aaggtgatag cgttgatgat tcttcattgg tcagaaaatt atgaacggtt
     5641 tcttctattt tgtctctata tactacgtat aggaaatgtt tacattttcg tattgttttc
     5701 gattcactct atgaatagtt cttactacaa tttttttgtc taaagagtaa tactagagat
     5761 aaacataaaa aatgtagagg tcgagtttag atgcaagttc aaggagcgaa aggtggatgg
     5821 gtaggttata tagggatata gcacagagat atatagcaaa gagatacttt tgagcaatgt
     5881 ttgtggaagc ggtattcgca atattttagt agctcgttac agtccggtgc gtttttggtt
     5941 ttttgaaagt gcgtcttcag agcgcttttg gttttcaaaa gcgctctgaa gttcctatac
     6001 tttctagcta gagaatagga acttcggaat aggaacttca aagcgtttcc gaaaacgagc
     6061 gcttccgaaa atgcaacgcg agctgcgcac atgcagctca ctgttcacgt cgcacctata
     6121 tctgcgtgtt gcctgtatat atatatacat gagaagaacg gcatagtgcg tgtttatgct
     6181 taaatgcgta cttatatgcg tctatttatg taggatgaaa ggtagtctag tacctcctgt
     6241 gatattatcc cattccatgc ggggtatcgt atgcttcctt cagcactacc ctttagctgt
     6301 tctatatgct gccactcctc aattggatta gtctcatcct tcaatgctat catttccttt
     6361 gatattggat catactaaga aaccattatt atcatgacat taacctataa aaataggcgt
     6421 atcacgaggc cctttcgtct cgcgcgtttc ggtgatgacg gtgaaaacct ctgacgcatg
     6481 cagctcccgg agacggtcac agcttgtctg taagcggatg ccgggagcag acaagcccgt
     6541 cagggcgcgt cagcgggtgt tggcgggtgt cggggctggc ttaactatgc ggcatcagag
     6601 cagattgtac tgagagtgca ccataccacc ttagcttgcc tcgtccccgc cgggtcaccc
     6661 ggccagcgac atggaggccc agaataccct ccttgacagt cttgacgtgc gcagctcagg
     6721 ggcatgatgt gactgtcgcc cgtacattta gcccatacat ccccatgtat aatcatttgc
     6781 atccatacat tttgatggcc gcacggcgcg aagcaaaaat tacggctcct cgctgcggac
     6841 ctgcgagcag ggaaacgctc ccctcacaga cgcgttgaat tgtccccacg ccgcgcccct
     6901 gtagagaaat ataaaaggtt aggatttgcc actgaggttc ttctttcata tacttccttt
     6961 taaaatcttg ctaggataca gttctcacat cacatccgaa cataaacaac catgggtaaa
     7021 aagcctgaac tcaccgcgac gtctgtcgag aagtttctga tcgaaaagtt cgacagcgtc
     7081 tccgacctga tgcagctctc ggagggcgaa gaatctcgtg ctttcagctt cgatgtagga
     7141 gggcgt
//
