LOCUS       Exported               11636 bp ds-DNA     circular SYN 16-NOV-2022
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11636)
  AUTHORS   Kayoko Tsukita
  TITLE     Direct Submission
  JOURNAL   Exported Wednesday, Nov 16, 2022 from SnapGene 5.2.5
            https://www.snapgene.com
COMMENT     Homo sapiens neurogenic differentiation 1 (NEUROD1), mRNA 
            NCBI Reference Sequence: NM_002500.5
FEATURES             Location/Qualifiers
     source          1..11636
                     /organism="recombinant plasmid"
                     /mol_type="other DNA"
     rep_origin      complement(3..458)
                     /direction=LEFT
                     /label=M13 origin
     rep_origin      13..453
                     /label=F1 ori
     CDS             complement(463..531)
                     /label=LacZ alpha
     primer_bind     602..619
                     /label=M13-fwd
     primer_bind     602..619
                     /label=M13-fwd
     primer_bind     618..645
                     /label=T7
     primer_bind     618..645
                     /label=T7
     misc_recomb     complement(707..941)
                     /label=PB 3'LTR
     misc_feature    1109..1116
                     /label=PacI
     misc_feature    1145..1163
                     /label=tetO2
                     /label=tetO2(1)
     misc_feature    1182..1200
                     /label=tetO2
                     /label=tetO2(2)
     misc_feature    1219..1237
                     /label=tetO2
                     /label=tetO2(3)
     misc_feature    1256..1274
                     /label=tetO2
                     /label=tetO2(4)
     misc_feature    1293..1311
                     /label=tetO2
                     /label=tetO2(5)
     misc_feature    1330..1348
                     /label=tetO2
                     /label=tetO2(6)
     misc_feature    1367..1385
                     /label=tetO2
                     /label=tetO2(7)
     misc_feature    1404..1422
                     /label=tetO2
     misc_feature    1431..1536
                     /label=mCMV promoter
                     /label=mCMV promoter(1)
     misc_feature    1431..1530
                     /label=mCMV promoter
     protein_bind    1548..1572
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_recomb     1548..1555
                     /label=AttR1
     CDS             1610..2506
                     /codon_start=1
                     /gene="CRX"
                     /gene_synonym="CORD2; CRD; LCA7; OTX3"
                     /product="cone-rod homeobox protein"
                     /label=CRX
                     /note="orthodenticle homeobox 3"
                     /db_xref="CCDS:CCDS12706.1"
                     /db_xref="GeneID:1406"
                     /db_xref="HGNC:HGNC:2383"
                     /db_xref="MIM:602225"
                     /protein_id="NP_000545.1"
                     /translation="MMAYMNPGPHYSVNALALSGPSVDLMHQAVPYPSAPRKQRRERTT
                     FTRSQLEELEALFAKTQYPDVYAREEVALKINLPESRVQVWFKNRRAKCRQQRQQQKQQ
                     QQPPGGQAKARPAKRKAGTSPRPSTDVCPDPLGISDSYSPPLPGPSGSPTTAVATVSIW
                     SPASESPLPEAQRAGLVASGPSLTSAPYAMTYAPASAFCSSPSAYGSPSSYFSGLDPYL
                     SPMVPQLGGPALSPLSGPSVGPSLAQSPTSLSGQSYGAYSPVDSLEFKDPTGTWKFTYN
                     PMDPLDYKDQSAWKFQIL"
     gene            1610..2506
                     /gene="CRX"
                     /gene_synonym="CORD2; CRD; LCA7; OTX3"
                     /label=CRX
                     /note="cone-rod homeobox"
                     /db_xref="GeneID:1406"
                     /db_xref="HGNC:HGNC:2383"
                     /db_xref="MIM:602225"
     exon            1610..1709
                     /gene="CRX"
                     /gene_synonym="CORD2; CRD; LCA7; OTX3"
                     /inference="alignment:same species:2.1.0"
     exon            1710..1861
                     /gene="CRX"
                     /gene_synonym="CORD2; CRD; LCA7; OTX3"
                     /inference="alignment:same species:2.1.0"
     exon            1862..2506
                     /gene="CRX"
                     /gene_synonym="CORD2; CRD; LCA7; OTX3"
                     /inference="alignment:same species:2.1.0"
     misc_feature    2507..2560
                     /locus_tag="T2A"
                     /label=T2A
     CDS             2561..3631
                     /codon_start=1
                     /gene="NEUROD1"
                     /gene_synonym="BETA2; BHF-1; bHLHa3; MODY6; NEUROD"
                     /product="neurogenic differentiation factor 1"
                     /label=NEUROD1
                     /note="neurogenic helix-loop-helix protein NEUROD; basic 
                     helix-loop-helix transcription factor; beta-cell E-box 
                     transactivator 2; class A basic helix-loop-helix protein 3"
                     /db_xref="CCDS:CCDS2283.1"
                     /db_xref="GeneID:4760"
                     /db_xref="HGNC:HGNC:7762"
                     /db_xref="MIM:601724"
                     /protein_id="NP_002491.2"
                     /translation="MTKSYSESGLMGEPQPQGPPSWTDECLSSQDEEHEADKKEDDLET
                     MNAEEDSLRNGGEEEDEDEDLEEEEEEEEEDDDQKPKRRGPKKKKMTKARLERFKLRRM
                     KANARERNRMHGLNAALDNLRKVVPCYSKTQKLSKIETLRLAKNYIWALSEILRSGKSP
                     DLVSFVQTLCKGLSQPTTNLVAGCLQLNPRTFLPEQNQDMPPHLPTASASFPVHPYSYQ
                     SPGLPSPPYGTMDSSHVFHVKPPPHAYSAALEPFFESPLTDCTSPSFDGPLSPPLSING
                     NFSFKHEPSAEFEKNYAFTMHYPAATLAGAQSHGSIFSGTAAPRCEIPIDNIMSFDSHS
                     HHERVMSAQLNAIFHD"
     exon            2561..3631
                     /gene="NEUROD1"
                     /gene_synonym="BETA2; BHF-1; bHLHa3; MODY6; NEUROD"
                     /inference="alignment:same species:2.1.0"
     gene            2561..3631
                     /gene="NEUROD1"
                     /gene_synonym="BETA2; BHF-1; bHLHa3; MODY6; NEUROD"
                     /label=NEUROD1
                     /note="neuronal differentiation 1"
                     /db_xref="GeneID:4760"
                     /db_xref="HGNC:HGNC:7762"
                     /db_xref="MIM:601724"
     STS             2561..2842
                     /gene="NEUROD1"
                     /gene_synonym="BETA2; BHF-1; bHLHa3; MODY6; NEUROD"
                     /standard_name="Neurod1"
                     /label=Neurod1
                     /db_xref="UniSTS:547737"
     misc_feature    2819..2839
                     /gene="NEUROD1"
                     /gene_synonym="BETA2; BHF-1; bHLHa3; MODY6; NEUROD"
                     /note="propagated from UniProtKB/Swiss-Prot (Q13562.3);
                     Region: Nuclear localization signal. {ECO:0000255}"
                     /experiment="experimental evidence, no additional details
                     recorded"
     misc_feature    3044..3046
                     /gene="NEUROD1"
                     /gene_synonym="BETA2; BHF-1; bHLHa3; MODY6; NEUROD"
                     /note="Phosphoserine. {ECO:0000250|UniProtKB:Q60867};
                     propagated from UniProtKB/Swiss-Prot (Q13562.3); 
                     phosphorylation site"
                     /experiment="experimental evidence, no additional details
                     recorded"
     STS             3123..3589
                     /gene="NEUROD1"
                     /gene_synonym="BETA2; BHF-1; bHLHa3; MODY6; NEUROD"
                     /standard_name="PMC317250P4"
                     /label=PMC317250P4
                     /db_xref="UniSTS:273087"
     misc_feature    3335..3337
                     /gene="NEUROD1"
                     /gene_synonym="BETA2; BHF-1; bHLHa3; MODY6; NEUROD"
                     /note="Phosphoserine. {ECO:0000250|UniProtKB:Q60867};
                     propagated from UniProtKB/Swiss-Prot (Q13562.3); 
                     phosphorylation site"
                     /experiment="experimental evidence, no additional details
                     recorded"
     misc_feature    3356..3358
                     /gene="NEUROD1"
                     /gene_synonym="BETA2; BHF-1; bHLHa3; MODY6; NEUROD"
                     /note="Phosphoserine. {ECO:0000250|UniProtKB:Q60867};
                     propagated from UniProtKB/Swiss-Prot (Q13562.3); 
                     phosphorylation site"
                     /experiment="experimental evidence, no additional details
                     recorded"
     misc_feature    3380..3382
                     /gene="NEUROD1"
                     /gene_synonym="BETA2; BHF-1; bHLHa3; MODY6; NEUROD"
                     /note="Phosphoserine. {ECO:0000250|UniProtKB:Q60867};
                     propagated from UniProtKB/Swiss-Prot (Q13562.3); 
                     phosphorylation site"
                     /experiment="experimental evidence, no additional details
                     recorded"
     STS             3394..3631
                     /gene="NEUROD1"
                     /gene_synonym="BETA2; BHF-1; bHLHa3; MODY6; NEUROD"
                     /standard_name="PMC85623P3"
                     /label=PMC85623P3
                     /db_xref="UniSTS:273480"
     misc_feature    3563..3565
                     /gene="NEUROD1"
                     /gene_synonym="BETA2; BHF-1; bHLHa3; MODY6; NEUROD"
                     /note="Phosphoserine, by CaMK2.
                     {ECO:0000250|UniProtKB:Q64289}; propagated from 
                     UniProtKB/Swiss-Prot (Q13562.3); phosphorylation site"
                     /experiment="experimental evidence, no additional details
                     recorded"
     protein_bind    complement(3641..3665)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_recomb     complement(3651..3665)
                     /label=AttR2
     polyA_site      3697..4237
                     /label=Rabbit b-globin pA
     misc_feature    complement(4324..4327)
                     /label=NheI
                     /note="/vntifkey=21"
     misc_feature    4339..4696
                     /label=pCAG promoter
                     /label=pCAG\promoter
                     /note="/vntifkey=21"
     misc_feature    4697..4708
                     /label=CAG_enhancer
     promoter        4821..6140
                     /label=rEF1a promoter
     CDS             6158..6904
                     /label=rtTA-Advanced
     RBS             6931..7398
                     /label=FMDV IRES
     promoter        7399..7472
                     /label=EM7 promoter
     CDS             7474..8268
                     /label=neoR (invivogen)
     polyA_site      8314..8888
                     /label=EF1 pA
     enhancer        8889..8890
                     /label=SV40 enhancer
     misc_recomb     complement(8987..9262)
                     /label=PB 5'LTR
     misc_recomb     complement(9263..9299)
                     /label=PB 5'LTR
                     /label=PB 5'LTR(1)
     primer_bind     complement(9443..9462)
                     /label=T3
     primer_bind     complement(9443..9462)
                     /label=T3
     primer_bind     complement(9480..9500)
                     /label=M13-rev
     primer_bind     complement(9480..9500)
                     /label=M13-rev
     misc_binding    complement(9506..9528)
                     /label=LacO
     rep_origin      complement(9868..10550)
                     /direction=LEFT
                     /label=ColE1 origin
     CDS             complement(10648..11307)
                     /label=AmpR
ORIGIN
        1 ggaaattgta aacgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc
       61 attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga
      121 gatagggttg agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc
      181 caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg aaccatcacc
      241 ctaatcaagt tttttggggt cgaggtgccg taaagcacta aatcggaacc ctaaagggag
      301 cccccgattt agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa
      361 agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac
      421 cacacccgcc gcgcttaatg cgccgctaca gggcgcgtcg cgccattcgc cattcaggct
      481 gcgcaactgt tgggaagggc gatcggtgcg ggcctcttcg ctattacgcc agctggcgaa
      541 agggggatgt gctgcaaggc gattaagttg ggtaacgcca gggttttccc agtcacgacg
      601 ttgtaaaacg acggccagtg aattgtaata cgactcacta tagggcgaat tggagctcgg
      661 tattcacgac agcaggctga ataataaaaa aattagaaac tattatttaa ccctagaaag
      721 ataatcatat tgtgacgtac gttaaagata atcatgcgta aaattgacgc atgtgtttta
      781 tcggtctgta tatcgaggtt tatttattaa tttgaataga tattaagttt tattatattt
      841 acacttacat actaataata aattcaacaa acaatttatt tatgtttatt tatttattaa
      901 aaaaaaacaa aaactcaaaa tttcttctat aaagtaacaa aacttttaaa cattctctct
      961 tttacaaaaa taaacttatt ttgtacttta aaaacagtca tgttgtatta taaaataagt
     1021 aattagctta acctatacat aatagaaaca aattatactt attagtcagt cagaaacaac
     1081 tttggcacat atcaatatta tgctctcgtt aattaactcg agggatccaa gctggccgca
     1141 cgtctcccta tcagtgatag agaagtcgac acgtctcgag ctccctatca gtgatagaga
     1201 aggtacgtct agaacgtctc cctatcagtg atagagaagt cgacacgtct cgagctccct
     1261 atcagtgata gagaaggtac gtctagaacg tctccctatc agtgatagag aagtcgacac
     1321 gtctcgagct ccctatcagt gatagagaag gtacgtctag aacgtctccc tatcagtgat
     1381 agagaagtcg acacgtctcg agctccctat cagtgataga gaaggtaccc cctatataag
     1441 cagagctcgt ttagtgaacc gtcagatcgc ctggagacgc catccacgct gttttgacct
     1501 ccatagaaga caccgggacc gatccagcct ccgcggtggc ggccatcaca agtttgtaca
     1561 aaaaagcagg ctttaaagga accaattcag tcgactggat ccggtaccga tgatggcgta
     1621 tatgaacccg gggccccact attctgtcaa cgccttggcc ctaagtggcc ccagtgtgga
     1681 tctgatgcac caggctgtgc cctacccaag cgcccccagg aagcagcggc gggagcgcac
     1741 caccttcacc cggagccaac tggaggagct ggaggcactg tttgccaaga cccagtaccc
     1801 agacgtctat gcccgtgagg aggtggctct gaagatcaat ctgcctgagt ccagggttca
     1861 ggtttggttc aagaaccgga gggctaaatg caggcagcag cgacagcagc agaaacagca
     1921 gcagcagccc ccagggggcc aggccaaggc ccggcctgcc aagaggaagg cgggcacgtc
     1981 cccaagaccc tccacagatg tgtgtccaga ccctctgggc atctcagatt cctacagtcc
     2041 ccctctgccc ggcccctcag gctccccaac cacggcagtg gccactgtgt ccatctggag
     2101 cccagcctca gagtcccctt tgcctgaggc gcagcgggct gggctggtgg cctcagggcc
     2161 gtctctgacc tccgccccct atgccatgac ctacgccccg gcctccgctt tctgctcttc
     2221 cccctccgcc tatgggtctc cgagctccta tttcagcggc ctagacccct acctttctcc
     2281 catggtgccc cagctagggg gcccggctct tagccccctc tctggcccct ccgtgggacc
     2341 ttccctggcc cagtccccca cctccctatc aggccagagc tatggcgcct acagccccgt
     2401 ggatagcttg gaattcaagg accccacggg cacctggaaa ttcacctaca atcccatgga
     2461 ccctctggac tacaaggatc agagtgcctg gaagtttcag atcttggagg gcagaggcag
     2521 cctgctgacc tgcggcgacg tggaggaaaa ccctggccct atgaccaaat cgtacagcga
     2581 gagtgggctg atgggcgagc ctcagcccca aggtcctcca agctggacag acgagtgtct
     2641 cagttctcag gacgaggagc acgaggcaga caagaaggag gacgacctcg aaaccatgaa
     2701 cgcagaggag gactcactga ggaacggggg agaggaggag gacgaagatg aggacctgga
     2761 agaggaggaa gaagaggaag aggaggatga cgatcaaaag cccaagagac gcggccccaa
     2821 aaagaagaag atgactaagg ctcgcctgga gcgttttaaa ttgagacgca tgaaggctaa
     2881 cgcccgggag cggaaccgca tgcacggact gaacgcggcg ctagacaacc tgcgcaaggt
     2941 ggtgccttgc tattctaaga cgcagaagct gtccaaaatc gagactctgc gcttggccaa
     3001 gaactacatc tgggctctgt cggagatcct gcgctcaggc aaaagcccag acctggtctc
     3061 cttcgttcag acgctttgca agggcttatc ccaacccacc accaacctgg ttgcgggctg
     3121 cctgcaactc aatcctcgga cttttctgcc tgagcagaac caggacatgc ccccccacct
     3181 gccgacggcc agcgcttcct tccctgtaca cccctactcc taccagtcgc ctgggctgcc
     3241 cagtccgcct tacggtacca tggacagctc ccatgtcttc cacgttaagc ctccgccgca
     3301 cgcctacagc gcagcgctgg agcccttctt tgaaagccct ctgactgatt gcaccagccc
     3361 ttcctttgat ggacccctca gcccgccgct cagcatcaat ggcaacttct ctttcaaaca
     3421 cgaaccgtcc gccgagtttg agaaaaatta tgcctttacc atgcactatc ctgcagcgac
     3481 actggcaggg gcccaaagcc acggatcaat cttctcaggc accgctgccc ctcgctgcga
     3541 gatccccata gacaatatta tgtccttcga tagccattca catcatgagc gagtcatgag
     3601 tgcccagctc aatgccatat ttcatgatta ggatatctag acccagcttt cttgtacaaa
     3661 gtggtgatgg ccgctctagc gaagggatcc cccatcgaat tcactcctca ggtgcaggct
     3721 gcctatcaga aggtggtggc tggtgtggcc aatgccctgg ctcacaaata ccactgagat
     3781 ctttttccct ctgccaaaaa ttatggggac atcatgaagc cccttgagca tctgacttct
     3841 ggctaataaa ggaaatttat tttcattgca atagtgtgtt ggaatttttt gtgtctctca
     3901 ctcggaagga catatgggag ggcaaatcat ttaaaacatc agaatgagta tttggtttag
     3961 agtttggcaa catatgccat atgctggctg ccatgaacaa aggtggctat aaagaggtca
     4021 tcagtatatg aaacagcccc ctgctgtcca ttccttattc catagaaaag ccttgacttg
     4081 aggttagatt ttttttatat tttgttttgt gttatttttt tctttaacat ccctaaaatt
     4141 ttccttacat gttttactag ccagattttt cctcctctcc tgactactcc cagtcatagc
     4201 tgtccctctt ctcttatgaa gatccctcga cctgcaggaa ttcgattact taagcctagg
     4261 atccagatct gtacattcga atcgatgaat tcgttaacca tggaagcttc tgcagcggcc
     4321 gcactagcgg tggcggcctc gacattgatt attgactagt tattaatagt aatcaattac
     4381 ggggtcatta gttcatagcc catatatgga gttccgcgtt acataactta cggtaaatgg
     4441 cccgcctggc tgaccgccca acgacccccg cccattgacg tcaataatga cgtatgttcc
     4501 catagtaacg ccaataggga ctttccattg acgtcaatgg gtggagtatt tacggtaaac
     4561 tgcccacttg gcagtacatc aagtgtatca tatgccaagt acgcccccta ttgacgtcaa
     4621 tgacggtaaa tggcccgcct ggcattatgc ccagtacatg accttatggg actttcctac
     4681 ttggcagtac atctacgtat tagtcatcgc tattaccatg atgatgcggt tttggcagta
     4741 catcaatggg cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga
     4801 cgtcaatggg agtttgtttt gactagtgga gccgagagta attcatacaa aaggagggat
     4861 cgccttcgca aggggagagc ccagggaccg tccctaaatt ctcacagacc caaatccctg
     4921 tagccgcccc acgacagcgc gaggagcatg cgcccagggc tgagcgcggg tagatcagag
     4981 cacacaagct cacagtcccc ggcggtgggg ggaggggcgc gctgagcggg ggccagggag
     5041 ctggcgcggg gcaaactggg aaagtggtgt cgtgtgctgg ctccgccctc ttcccgaggg
     5101 tgggggagaa cggtatataa gtgcggtagt cgccttggac gttctttttc gcaacgggtt
     5161 tgccgtcaga acgcaggtga gtggcgggtg tggcttccgc gggccccgga gctggagccc
     5221 tgctctgagc gggccgggct gatatgcgag tgtcgtccgc agggtttagc tgtgagcatt
     5281 cccacttcga gtggcgggcg gtgcgggggt gagagtgcga ggcctagcgg caaccccgta
     5341 gcctcgcctc gtgtccggct tgaggcctag cgtggtgtcc gccgccgcgt gccactccgg
     5401 ccgcactatg cgttttttgt ccttgctgcc ctcgattgcc ttccagcagc atgggctaac
     5461 aaagggaggg tgtggggctc actcttaagg agcccatgaa gcttacgttg gataggaatg
     5521 gaagggcagg aggggcgact ggggcccgcc cgccttcgga gcacatgtcc gacgccacct
     5581 ggatggggcg aggcctgtgg ctttccgaag caatcgggcg tgagtttagc ctacctgggc
     5641 catgtggccc tagcactggg cacggtctgg cctggcggtg ccgcgttccc ttgcctccca
     5701 acaagggtga ggccgtcccg cccggcacca gttgcttgcg cggaaagatg gccgctcccg
     5761 gggccctgtt gcaaggagct caaaatggag gacgcggcag cccggtggag cgggcgggtg
     5821 agtcacccac acaaaggaag agggccttgc ccctcgccgg ccgctgcttc ctgtgacccc
     5881 gtggtctatc ggccgcatag tcacctcggg cttctcttga gcaccgctcg tcgcggcggg
     5941 gggaggggat ctaatggcgt tggagtttgt tcacatttgg tgggtggaga ctagtcaggc
     6001 cagcctggcg ctggaagtca ttcttggaat ttgccccttt gagtttggag cgaggctaat
     6061 tctcaagcct cttagcggtt caaaggtatt ttctaaaccc gtttccaggt gttgtgaaag
     6121 ccaccgctaa ttcaaagcaa tccggagtaa attcaccatg tctagactgg acaagagcaa
     6181 agtcataaac ggcgctctgg aattactcaa tggagtcggt atcgaaggcc tgacgacaag
     6241 gaaactcgct caaaagctgg gagttgagca gcctaccctg tactggcacg tgaagaacaa
     6301 gcgggccctg ctcgatgccc tgccaatcga gatgctggac aggcatcata cccacttctg
     6361 ccccctggaa ggcgagtcat ggcaagactt tctgcggaac aacgccaagt cattccgctg
     6421 tgctctcctc tcacatcgcg acggggctaa agtgcatctc ggcacccgcc caacagagaa
     6481 acagtacgaa accctggaaa atcagctcgc gttcctgtgt cagcaaggct tctccctgga
     6541 gaacgcactg tacgctctgt ccgccgtggg ccactttaca ctgggctgcg tattggagga
     6601 acaggagcat caagtagcaa aagaggaaag agagacacct accaccgatt ctatgccccc
     6661 acttctgaga caagcaattg agctgttcga ccggcaggga gccgaacctg ccttcctttt
     6721 cggcctggaa ctaatcatat gtggcctgga gaaacagcta aagtgcgaaa gcggcgggcc
     6781 ggccgacgcc cttgacgatt ttgacttaga catgctccca gccgatgccc ttgacgactt
     6841 tgaccttgat atgctgcctg ctgacgctct tgacgatttt gaccttgaca tgctccccgg
     6901 gtaactaagt aaggatcccg tacgcctagg agcaggtttc cccaatgaca caaaacgtgc
     6961 aacttgaaac tccgcctggt ctttccaggt ctagaggggt aacactttgt actgcgtttg
     7021 gctccacgct cgatccactg gcgagtgtta gtaacagcac tgttgcttcg tagcggagca
     7081 tgacggccgt gggaactcct ccttggtaac aaggacccac ggggccaaaa gccacgccca
     7141 cacgggcccg tcatgtgtgc aaccccagca cggcgacttt actgcgaaac ccactttaaa
     7201 gtgacattga aactggtacc cacacactgg tgacaggcta aggatgccct tcaggtaccc
     7261 cgaggtaaca cgcgacactc gggatctgag aaggggactg gggcttctat aaaagcgctc
     7321 ggtttaaaaa gcttctatgc ctgaataggt gaccggaggt cggcaccttt cctttgcaat
     7381 tactgaccct atgaatacac tgactgtttg acaattaatc atcggcatag tatatcggca
     7441 tagtataata cgactcacta taggagggcc accatgattg aacaagatgg attgcacgca
     7501 ggttctccgg ccgcttgggt ggagaggcta ttcggctatg actgggcaca acagacaatc
     7561 ggctgctctg atgccgccgt gttccggctg tcagcgcagg ggcgcccggt tctttttgtc
     7621 aagaccgacc tgtccggtgc cctgaatgaa ctgcaagacg aggcagcgcg gctatcgtgg
     7681 ctggccacga cgggcgttcc ttgcgcagct gtgctcgacg ttgtcactga agcgggaagg
     7741 gactggctgc tattgggcga agtgccgggg caggatctcc tgtcatctca ccttgctcct
     7801 gccgagaaag tatccatcat ggctgatgca atgcggcggc tgcatacgct tgatccggct
     7861 acctgcccat tcgaccacca agcgaaacat cgcatcgagc gagcacgtac tcggatggaa
     7921 gccggtcttg tcgatcagga tgatctggac gaagagcatc aggggctcgc gccagccgaa
     7981 ctgttcgcca ggctcaaggc gagcatgccc gacggcgagg atctcgtcgt gacacatggc
     8041 gatgcctgct tgccgaatat catggtggaa aatggccgct tttctggatt catcgactgt
     8101 ggccggctgg gtgtggcgga ccgctatcag gacatagcgt tggctacccg tgatattgct
     8161 gaagagcttg gcggcgaatg ggctgaccgc ttcctcgtgc tttacggtat cgccgctccc
     8221 gattcgcagc gcatcgcctt ctatcgcctt cttgacgagt tcttctgagc gggactctgg
     8281 ggttcgaaat gaccgaccaa gcgaattcgc taggattatc cctaatacct gccaccccac
     8341 tcttaatcag tggtggaaga acggtctcag aactgtttgt ttcaattggc catttaagtt
     8401 tagtagtaaa agactggtta atgataacaa tgcatcgtaa aaccttcaga aggaaaggag
     8461 aatgttttgt ggaccacttt ggttttcttt tttgcgtgtg gcagttttaa gttattagtt
     8521 tttaaaatca gtacttttta atggaaacaa cttgaccaaa aatttgtcac agaattttga
     8581 gacccattaa aaaagttaaa tgagaaacct gtgtgttcct ttggtcaaca ccgagacatt
     8641 taggtgaaag acatctaatt ctggttttac gaatctggaa acttcttgaa aatgtaattc
     8701 ttgagttaac acttctgggt ggagaatagg gttgttttcc ccccacataa ttggaagggg
     8761 aaggaatatc atttaaagct atgggagggt tgctttgatt acaacactgg agagaaatgc
     8821 agcatgttgc tgattgcctg tcactaaaac aggccaaaaa ctgagtcctt gggttgcata
     8881 gaaagctgcc aaacgctagc caacaagctc gtcatcgctt tgcagaagag cagagaggat
     8941 atgctcatcg tctaaagaac tacccatttt attatatatt agtcacgata tctataacaa
     9001 gaaaatatat atataataag ttatcacgta agtagaacat gaaataacaa tataattatc
     9061 gtatgagtta aatcttaaaa gtcacgtaaa agataatcat gcgtcatttt gactcacgcg
     9121 gtcgttatag ttcaaaatca gtgacactta ccgcattgac aagcacgcct cacgggagct
     9181 ccaagcggcg actgagatgt cctaaatgca cagcgacgga ttcgcgctat ttagaaagag
     9241 agagcaatat ttcaagaatg catgcgtcaa ttttacgcag actatctttc tagggttaaa
     9301 aaagatttgc gctttactcg acctaaactt taaacacgtc atagaatctt cgtttgacaa
     9361 aaaccacatt gtggccaagc tgtgtgacgc gacgcgcgct aaagaatggc aaaccaagtc
     9421 gcgcgaggta cccagctttt gttcccttta gtgagggtta attccgagct tggcgtaatc
     9481 atggtcatag ctgtttcctg tgtgaaattg ttatccgctc acaattccac acaacatacg
     9541 agccggaagc ataaagtgta aagcctgggg tgcctaatga gtgagctaac tcacattaat
     9601 tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg tcgtgccagc tgcattaatg
     9661 aatcggccaa cgcgcgggga gaggcggttt gcgtattggg cgctcttccg cttcctcgct
     9721 cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc
     9781 ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg
     9841 ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg
     9901 cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg
     9961 actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac
    10021 cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca
    10081 tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt
    10141 gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc
    10201 caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag
    10261 agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac
    10321 tagaaggaca gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt
    10381 tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa
    10441 gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg
    10501 gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa
    10561 aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat
    10621 atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc
    10681 gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat
    10741 acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc
    10801 ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc
    10861 tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag
    10921 ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg tggtgtcacg
    10981 ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg
    11041 atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag
    11101 taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt
    11161 catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga
    11221 atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc
    11281 acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc
    11341 aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc
    11401 ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc
    11461 cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca
    11521 atattattga agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat
    11581 ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctg
//
