LOCUS       Cterm_AAV_ABEmax-NG_403Q_mouse_guide_cTNT_promoter_Corrected_Guide        7348 bp ds-DNA     circular SYN 16-SEP-2022
DEFINITION  .
ACCESSION
VERSION     .
KEYWORDS    pX601-GFP.
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct.
REFERENCE   1  (bases 1 to 8138)
  AUTHORS   Ye L, Wang J, Tan Y, Beyer AI, Xie F, Muench MO and Kan YW
  TITLE     Genome editing using CRISPR-Cas9 to create the HPFH genotype in
            HSPCs: An approach for treating sickle cell disease and
            beta-thalassemia.
  JOURNAL   Proc Natl Acad Sci U S A. 2016 Sep 20;113(38):10661-5. doi:
            10.1073/pnas.1612075113. Epub 2016 Sep 6.
  PUBMED    27601644
REFERENCE   2  (bases 1 to 8138)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Tuesday, Jun 20, 2017 from SnapGene Console 4.0.0a8
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     misc_feature    3..2954
                     /note="Geneious type: Editing History Insertion"
                     /standard_name
     misc_feature    122..282
                     /note="Geneious type: reporter"
                     /standard_name="lacZ_a reporter"
     primer_bind     complement(125..142)
                     /standard_name="Eton M13F(-21)"
     primer_bind     complement(125..142)
                     /standard_name="Genewiz M13F(-21)"
     primer_bind     complement(125..142)
                     /standard_name="QB0046 M13 (-21) Forward"
     primer_bind     complement(125..141)
                     /standard_name="M13-F (-20)"
     primer_bind     complement(134..156)
                     /standard_name="QB0049 M13/pUC Forward"
     primer_bind     complement(145..168)
                     /standard_name="Genewiz M13F(-47)"
     primer_bind     complement(145..162)
                     /standard_name="Genewiz M13F(-41)"
     primer_bind     complement(145..161)
                     /standard_name="Eton M13F(-40)"
     primer_bind     complement(145..161)
                     /standard_name="M13-F (-40)"
     primer_bind     complement(145..161)
                     /standard_name="QB0047 M13 (-40)"
     primer_bind     complement(146..167)
                     /standard_name="M13-F (-46)"
     primer_bind     complement(209..228)
                     /standard_name="LacZ-R (EtonSeq)"
     primer_bind     209..228
                     /standard_name="834_LacZ-fwd1"
     misc_feature    300..606
                     /note="Geneious type: origin of replication"
                     /standard_name="f1 origin"
     primer_bind     complement(420..439)
                     /standard_name="835_LacZ-rev1"
     regulatory      799..827
                     /note="Geneious type: promoter"
                     /standard_name="amp prom"
     misc_feature    869..1729
                     /note="Geneious type: marker"
                     /standard_name="amp marker"
     CDS             869..1729
                     /standard_name="Amp-R"
     primer_bind     complement(994..1021)
                     /standard_name="563_Amp_Rev"
     primer_bind     994..1021
                     /standard_name="562_Amp_Fwd"
     primer_bind     complement(1087..1106)
                     /standard_name="QB0005 Amp-R"
     primer_bind     complement(1304..1323)
                     /standard_name="ACK563"
     misc_feature    1884..2503
                     /note="Geneious type: origin of replication"
                     /standard_name="pBR322 origin"
     primer_bind     1968..1987
                     /standard_name="832_BR322-fwd1"
     primer_bind     2121..2140
                     /standard_name="QB0767 RepO-R"
     primer_bind     complement(2149..2168)
                     /standard_name="833_BR322-rev1"
     primer_bind     complement(2247..2265)
                     /standard_name="QB0766 RepO-F"
     primer_bind     2408..2427
                     /standard_name="BR322-F"
     primer_bind     2497..2518
                     /standard_name="11_pFYF_Seq_Rev"
     primer_bind     2588..2607
                     /standard_name="VR"
     primer_bind     2642..2659
                     /standard_name="QB0039 L4440"
     primer_bind     2786..2805
                     /standard_name="Lac-F (EtonSeq)"
     primer_bind     2786..2805
                     /standard_name="836_Lac-F"
     regulatory      2812..2841
                     /note="Geneious type: promoter"
                     /standard_name="lac prom"
     primer_bind     2854..2877
                     /standard_name="M13-R (-46)"
     primer_bind     2854..2877
                     /standard_name="QB0108 Tac promoter"
     primer_bind     2855..2877
                     /standard_name="QB0050 M13/pUC Reverse"
     primer_bind     2874..2890
                     /standard_name="M13-R (-26)"
     primer_bind     2874..2890
                     /standard_name="Genewiz M13R"
     primer_bind     2874..2890
                     /standard_name="Eton M13R Reverse"
     primer_bind     2874..2890
                     /standard_name="QB0048 M13 Reverse"
     primer_bind     complement(2876..2902)
                     /standard_name="654_CAG_fwd"
     repeat_region   2917..3046
                     /standard_name="5'-ITR pSub201(+)"
     misc_RNA        3059..3500
                     /standard_name="ctnt Gallus gallus troponin T type 2
                     promoter"
     misc_feature    3059..6941
                     /note="Geneious type: Editing History Insertion"
                     /standard_name
     misc_feature    3075..3095
                     /note="Geneious type: primer_bind_reverse"
                     /standard_name="Forward sequencing primer1 cTNT"
     misc_feature    complement(3140..3158)
                     /note="Geneious type: primer_bind_reverse"
                     /standard_name="Reverse sequencing primer"
     misc_feature    3572..3594
                     /note="Geneious type: primer_bind_reverse"
                     /standard_name="Forward sequencing primer 2 cTNT"
     misc_RNA        3617..3749
                     /standard_name="Intron 1"
     misc_feature    3809..3830
                     /note="Geneious type: primer_bind_reverse"
                     /standard_name="Forward sequencing primer 3 cTNT"
     misc_feature    3848..3904
                     /note="Geneious type: primer_bind_reverse"
                     /standard_name="BPNLS"
     CDS             3905..4009
                     /standard_name="NpuC"
     primer_bind     complement(3985..4029)
                     /standard_name="902_fzCas9_NpuN-rev"
     source          4010..>4032
     CDS             <4010..>4032
                     /codon_start=1
                     /note="hSpCas9"
                     /standard_name="fz hSpCas9"
     primer_bind     4010..4032
                     /standard_name="841_FZ_Cas9-C11-fwd"
     CDS             <4033..6394
                     /codon_start=2
                     /note="hSpCas9"
                     /standard_name="fz hSpCas9 VRVRFRR"
     primer_bind     4412..4431
                     /standard_name="LCv2_seq14"
     primer_bind     4905..4924
                     /standard_name="LCv2_seq15"
     variation       5041
                     /standard_name="Silent C>T polymorphism"
     primer_bind     5370..5388
                     /standard_name="LCv2_seq16"
     misc_feature    5621..5623
                     /note="Geneious type: primer_bind_reverse"
                     /standard_name="L1111R"
     misc_feature    5693..5695
                     /note="Geneious type: primer_bind_reverse"
                     /standard_name="D1135V"
     primer_bind     5920..5939
                     /standard_name="LCv2_seq17"
     misc_feature    5942..5944
                     /note="Geneious type: primer_bind_reverse"
                     /standard_name="G1218R"
     misc_feature    5945..5947
                     /note="Geneious type: primer_bind_reverse"
                     /standard_name="E1219F"
     misc_feature    6254..6256
                     /note="Geneious type: primer_bind_reverse"
                     /standard_name="A1322R"
     misc_feature    6293..6295
                     /note="Geneious type: primer_bind_reverse"
                     /standard_name="R1335V"
     misc_feature    6299..6301
                     /note="Geneious type: primer_bind_reverse"
                     /standard_name="T1337R"
     source          <6395..6406
     CDS             6407..>6410
                     /standard_name="BPNLS"
     misc_feature    <6411..>6436
                     /standard_name="SV40 NLS"
     CDS             <6411..>6436
                     /codon_start=3
                     /standard_name="UGI"
     primer_bind     complement(6426..6484)
                     /standard_name="670_CW3_BPNLS_rev"
     misc_feature    6437..6457
                     /standard_name="SV40 NLS"
     primer_bind     complement(6450..6484)
                     /standard_name="814_Cas9-W3-rev"
     primer_bind     complement(6455..6484)
                     /standard_name="815_W3-Cas9-FLAG-rev"
     primer_bind     6465..6496
                     /standard_name="381_pAAV_fwd"
     regulatory      6466..6626
                     /note="Geneious type: terminator"
                     /standard_name="WPRE_Gamma"
     source          <6466..>6714
                     /organism="Cloning vector pAAV-CW3SL-EGFP"
                     /mol_type="other DNA"
                     /db_xref="taxon:1476495"
     primer_bind     complement(6522..6542)
                     /standard_name="QB0112 WPRE-R"
     primer_bind     complement(6544..6561)
                     /standard_name="pWPTRev"
     primer_bind     complement(6694..6724)
                     /standard_name="412_CW3/bGH_rev"
     primer_bind     6705..6737
                     /standard_name="411_CW3/bGH_fwd"
     misc_feature    <6715..6925
                     /note="Geneious type: polyA_signal"
                     /standard_name="BGH\pA"
     primer_bind     6715..6735
                     /standard_name="435_bGH_QPCRF1"
     primer_bind     6741..6759
                     /standard_name="436_bGH_QPCRF2"
     primer_bind     complement(6864..6888)
                     /standard_name="ACK64 (end)"
     primer_bind     complement(6878..6897)
                     /standard_name="438_bGH_QPCRR2"
     primer_bind     complement(6883..6901)
                     /standard_name="439_bGH_QPCRR3"
     primer_bind     complement(6903..6922)
                     /standard_name="437_bGH_QPCRR1"
     primer_bind     6923..6971
                     /standard_name="924_3'U6-XhoI-fwd"
     primer_bind     complement(6923..6942)
                     /standard_name="920_bGH-BamHI-rev"
     misc_feature    6943..7052
                     /note="Geneious type: Editing History Insertion"
                     /standard_name
     regulatory      complement(6949..6955)
                     /note="Geneious type: terminator"
                     /standard_name="U6 terminator"
     misc_feature    6949..6969
                     /note="Geneious type: primer_bind_reverse"
                     /standard_name="ACK68"
     primer_bind     6949..6969
                     /standard_name="223_rev_sgRNA_T7"
     ncRNA           complement(6956..7031)
                     /standard_name="RNA scaffold"
     primer_bind     complement(6997..7031)
                     /standard_name="ACK337-343 anneal"
     primer_bind     complement(7011..7031)
                     /standard_name="ACK74-78,144,148 anneal"
     regulatory      complement(7053..7316)
                     /note="Geneious type: promoter"
                     /standard_name="hU6"
     primer_bind     7053..7074
                     /standard_name="Rev_sgRNA_plasmid"
     primer_bind     complement(7111..7130)
                     /standard_name="Eton LKO.1 5' (hU6 promoter)"
     primer_bind     complement(7111..7130)
                     /standard_name="QB0042 LKO.1 5'"
     primer_bind     complement(7150..7172)
                     /standard_name="LCv2_seq5"
     primer_bind     complement(7175..7194)
                     /standard_name="ACK365"
     primer_bind     complement(7175..7194)
                     /standard_name="9_ACK365"
     primer_bind     complement(7297..7342)
                     /standard_name="913_3'U6-NotI-rev"
     misc_feature    7317..7324
                     /note="Geneious type: Editing History Insertion"
                     /standard_name
     repeat_region   complement(7325..106)
                     /standard_name="3'-ITR pSub201(+)"
ORIGIN      
        1 ccactccctc tctgcgcgct cgctcgctca ctgaggccgg gcgaccaaag gtcgcccgac
       61 gcccgggctt tgcccgggcg gcctcagtga gcgagcgagc gcgcagcctt aattaaccta
      121 attcactggc cgtcgtttta caacgtcgtg actgggaaaa ccctggcgtt acccaactta
      181 atcgccttgc agcacatccc cctttcgcca gctggcgtaa tagcgaagag gcccgcaccg
      241 atcgcccttc ccaacagttg cgcagcctga atggcgaatg ggacgcgccc tgtagcggcg
      301 cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt gccagcgccc
      361 tagcgcccgc tcctttcgct ttcttccctt cctttctcgc cacgttcgcc ggctttcccc
      421 gtcaagctct aaatcggggg ctccctttag ggttccgatt tagtgcttta cggcacctcg
      481 accccaaaaa acttgattag ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg
      541 tttttcgccc tttgacgttg gagtccacgt tctttaatag tggactcttg ttccaaactg
      601 gaacaacact caaccctatc tcggtctatt cttttgattt ataagggatt ttgccgattt
      661 cggcctattg gttaaaaaat gagctgattt aacaaaaatt taacgcgaat tttaacaaaa
      721 tattaacgct tacaatttag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg
      781 tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat
      841 gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat
      901 tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt
      961 aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag
     1021 cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa
     1081 agttctgcta tgtggcgcgg tattatcccg tattgacgcc gggcaagagc aactcggtcg
     1141 ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct
     1201 tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac
     1261 tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca
     1321 caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat
     1381 accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact
     1441 attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc
     1501 ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga
     1561 taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg
     1621 taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg
     1681 aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca
     1741 agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta
     1801 ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca
     1861 ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg
     1921 cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga
     1981 tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa
     2041 tactgttctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc
     2101 tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg
     2161 tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac
     2221 ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct
     2281 acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc
     2341 ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg
     2401 gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg
     2461 ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct
     2521 ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga
     2581 taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg
     2641 cagcgagtca gtgagcgagg aagcggaaga gcgcccaata cgcaaaccgc ctctccccgc
     2701 gcgttggccg attcattaat gcagctggca cgacaggttt cccgactgga aagcgggcag
     2761 tgagcgcaac gcaattaatg tgagttagct cactcattag gcaccccagg ctttacactt
     2821 tatgcttccg gctcgtatgt tgtgtggaat tgtgagcgga taacaatttc acacaggaaa
     2881 cagctatgac catgattacg ccagatttaa ttaaggctgc gcgctcgctc gctcactgag
     2941 gccgcccggg caaagcccgg gcgtcgggcg acctttggtc gcccggcctc agtgagcgag
     3001 cgagcgcgca gagagggagt ggccaactcc atcactaggg gttcctgcgg cctctagacg
     3061 ggccccccct cgaggtcggg ataaaagcag tctgggcttt cacatgacag catctggggc
     3121 tgcggcagag ggtcgggtcc gaagcgctgc cttatcagcg tccccagccc tgggaggtga
     3181 cagctggctg gcttgtgtca gcccctcggg cactcacgta tctccgtccg acgggtttaa
     3241 aatagcaaaa ctctgaggcc acacaatagc ttgggcttat atgggctcct gtgggggaag
     3301 ggggagcacg gagggggccg gggccgctgc tgccaaaata gcagctcaca agtgttgcat
     3361 tcctctctgg gcgccgggca cattcctgct ggctctgccc gccccggggt gggcgccggg
     3421 gggaccttaa agcctctgcc ccccaaggag cccttcccag acagccgccg gcacccaccg
     3481 ctccgtggga cgatccccga agctctagag ctttattgcg gtagtttatc acagttaaat
     3541 tgctaacgca gtcagtgctt ctgacacaac agtctcgaac ttaagctgca gaagttggtc
     3601 gtgaggcact gggcaggtaa gtatcaaggt tacaagacag gtttaaggag accaatagaa
     3661 actgggcttg tcgagacaga gaagactctt gcgtttctga taggcaccta ttggtcttac
     3721 tgacatccac tttgcctttc tctccacagg tgtccactcc cagttcaatt acagctctta
     3781 aggctagagt acttaatacg actcactata ggctagcctc gagaattcac gcgggaccgg
     3841 tgccaccatg aaacggacag ccgacggaag cgagttcgag tcaccaaaga agaagcggaa
     3901 agtcatcaag attgctacac ggaaatacct gggaaagcag aacgtgtacg acatcggcgt
     3961 ggagcgggat cacaacttcg ccctgaagaa tggctttatc gccagcaatt gcttcgactc
     4021 cgtggaaatc tccggcgtgg aagatcggtt caacgcctcc ctgggcacat accacgatct
     4081 gctgaaaatt atcaaggaca aggacttcct ggacaatgag gaaaacgagg acattctgga
     4141 agatatcgtg ctgaccctga cactgtttga ggacagagag atgatcgagg aacggctgaa
     4201 aacctatgcc cacctgttcg acgacaaagt gatgaagcag ctgaagcggc ggagatacac
     4261 cggctggggc aggctgagcc ggaagctgat caacggcatc cgggacaagc agtccggcaa
     4321 gacaatcctg gatttcctga agtccgacgg cttcgccaac agaaacttca tgcagctgat
     4381 ccacgacgac agcctgacct ttaaagagga catccagaaa gcccaggtgt ccggccaggg
     4441 cgatagcctg cacgagcaca ttgccaatct ggccggcagc cccgccatta agaagggcat
     4501 cctgcagaca gtgaaggtgg tggacgagct cgtgaaagtg atgggccggc acaagcccga
     4561 gaacatcgtg atcgaaatgg ccagagagaa ccagaccacc cagaagggac agaagaacag
     4621 ccgcgagaga atgaagcgga tcgaagaggg catcaaagag ctgggcagcc agatcctgaa
     4681 agaacacccc gtggaaaaca cccagctgca gaacgagaag ctgtacctgt actacctgca
     4741 gaatgggcgg gatatgtacg tggaccagga actggacatc aaccggctgt ccgactacga
     4801 tgtggaccat atcgtgcctc agagctttct gaaggacgac tccatcgaca acaaggtgct
     4861 gaccagaagc gacaagaacc ggggcaagag cgacaacgtg ccctccgaag aggtcgtgaa
     4921 gaagatgaag aactactggc ggcagctgct gaacgccaag ctgattaccc agagaaagtt
     4981 cgacaatctg accaaggccg agagaggcgg cctgagcgaa ctggataagg ccggcttcat
     5041 taagagacag ctggtggaaa cccggcagat cacaaagcac gtggcacaga tcctggactc
     5101 ccggatgaac actaagtacg acgagaatga caagctgatc cgggaagtga aagtgatcac
     5161 cctgaagtcc aagctggtgt ccgatttccg gaaggatttc cagttttaca aagtgcgcga
     5221 gatcaacaac taccaccacg cccacgacgc ctacctgaac gccgtcgtgg gaaccgccct
     5281 gatcaaaaag taccctaagc tggaaagcga gttcgtgtac ggcgactaca aggtgtacga
     5341 cgtgcggaag atgatcgcca agagcgagca ggaaatcggc aaggctaccg ccaagtactt
     5401 cttctacagc aacatcatga actttttcaa gaccgagatt accctggcca acggcgagat
     5461 ccggaagcgg cctctgatcg agacaaacgg cgaaaccggg gagatcgtgt gggataaggg
     5521 ccgggatttt gccaccgtgc ggaaagtgct gagcatgccc caagtgaata tcgtgaaaaa
     5581 gaccgaggtg cagacaggcg gcttcagcaa agagtctatc cggcccaaga ggaacagcga
     5641 taagctgatc gccagaaaga aggactggga ccctaagaag tacggcggct tcgtgagccc
     5701 caccgtggcc tattctgtgc tggtggtggc caaagtggaa aagggcaagt ccaagaaact
     5761 gaagagtgtg aaagagctgc tggggatcac catcatggaa agaagcagct tcgagaagaa
     5821 tcccatcgac tttctggaag ccaagggcta caaagaagtg aaaaaggacc tgatcatcaa
     5881 gctgcctaag tactccctgt tcgagctgga aaacggccgg aagagaatgc tggcctctgc
     5941 ccggttcctg cagaagggaa acgaactggc cctgccctcc aaatatgtga acttcctgta
     6001 cctggccagc cactatgaga agctgaaggg ctcccccgag gataatgagc agaaacagct
     6061 gtttgtggaa cagcacaagc actacctgga cgagatcatc gagcagatca gcgagttctc
     6121 caagagagtg atcctggccg acgctaatct ggacaaagtg ctgtccgcct acaacaagca
     6181 ccgggataag cccatcagag agcaggccga gaatatcatc cacctgttta ccctgaccaa
     6241 tctgggagcc cctcgggcct tcaagtactt tgacaccacc atcgaccgga aggtgtaccg
     6301 gagcaccaaa gaggtgctgg acgccaccct gatccaccag agcatcaccg gcctgtacga
     6361 gacacggatc gacctgtctc agctgggagg tgactctggc ggctcaaaaa gaaccgccga
     6421 cggcagcgaa ttcgagccca agaagaagag gaaagtctaa gatctgataa tcaacctctg
     6481 gattacaaaa tttgtgaaag attgactggt attcttaact atgttgctcc ttttacgcta
     6541 tgtggatacg ctgctttaat gcctttgtat catgctattg cttcccgtat ggctttcatt
     6601 ttctcctcct tgtataaatc ctggttagtt cttgccacgg cggaactcat cgccgcctgc
     6661 cttgcccgct gctggacagg ggctcggctg ttgggcactg acaattccgt ggtgcgactg
     6721 tgccttctag ttgccagcca tctgttgttt gcccctcccc cgtgccttcc ttgaccctgg
     6781 aaggtgccac tcccactgtc ctttcctaat aaaatgagga aattgcatcg cattgtctga
     6841 gtaggtgtca ttctattctg gggggtgggg tggggcagga cagcaagggg gaggattggg
     6901 aagacaatag caggcatgct ggggatgcgg tgggctctat ggctcgagaa aaaaagcacc
     6961 gactcggtgc cactttttca agttgataac ggactagcct tattttaact tgctatttct
     7021 agctctaaaa cttccccacc ttcacctgag gcggtgtttc gtcctttcca caagatatat
     7081 aaagccaaga aatcgaaata ctttcaagtt acggtaagca tatgatagtc cattttaaaa
     7141 cataatttta aaactgcaaa ctacccaaga aattattact ttctacgtca cgtattttgt
     7201 actaatatct ttgtgtttac agtcaaatta attctaatta tctctctaac agccttgtat
     7261 cgtatatgca aatatgaagg aatcatggga aataggccct cttcctgccc gaccttgcgg
     7321 ccgcaggaac ccctagtgat ggagttgg
//
