LOCUS       Exported                 870 bp DNA     linear   UNA 19-NOV-2022
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 870)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Nov 19, 2022 from SnapGene Viewer 6.0.5
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..870
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    1..15
                     /label=Cloning site in pUC19
     CDS             16..42
                     /label=Linker
     CDS             43..108
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             109..801
                     /codon_start=1
                     /product="bright monomeric red fluorescent protein evolved 
                     from a synthetic template (Bindels et al., 2016)"
                     /label=mScarlet
                     /translation="VSKGEAVIKEFMRFKVHMEGSMNGHEFEIEGEGEGRPYEGTQTAK
                     LKVTKGGPLPFSWDILSPQFMYGSRAFTKHPADIPDYYKQSFPEGFKWERVMNFEDGGA
                     VTVTQDTSLEDGTLIYKVKLRGTNFPPDGPVMQKKTMGWEASTERLYPEDGVLKGDIKM
                     ALRLKDGGRYLADFKTTYKAKKPVQMPGAYNVDRKLDITSHNEDYTVVEQYERSEGRHS
                     TGGMDELYK"
     CDS             802..828
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HAtag
                     /translation="YPYDVPDYA"
     CDS             829..855
                     /label=Linker
     misc_feature    856..870
                     /label=Cloning site in pUC19
ORIGIN
        1 cggtacccgg ggatctctag cggaccttca ggtagttcga gcgactacaa ggaccacgac
       61 ggggattata aagatcatga tatagattat aaagatgatg atgataaagt gagcaagggc
      121 gaggcagtga tcaaggagtt catgcggttc aaggtgcaca tggagggctc catgaacggc
      181 cacgagttcg agatcgaggg cgagggcgag ggccgcccct acgagggcac ccagaccgcc
      241 aagctgaagg tgaccaaggg tggccccctg cccttctcct gggacatcct gtcccctcag
      301 ttcatgtacg gctccagggc cttcaccaag caccccgccg acatccccga ctactataag
      361 cagtccttcc ccgagggctt caagtgggag cgcgtgatga acttcgagga cggcggcgcc
      421 gtgaccgtga cccaggacac ctccctggag gacggcaccc tgatctacaa ggtgaagctc
      481 cgcggcacca acttccctcc tgacggcccc gtaatgcaga agaagacaat gggctgggaa
      541 gcatccaccg agcggttgta ccccgaggac ggcgtgctga agggcgacat taagatggcc
      601 ctgcgcctga aggacggcgg ccgctacctg gcggacttca agaccaccta caaggccaag
      661 aagcccgtgc agatgcccgg cgcctacaac gtcgaccgca agttggacat cacctcccac
      721 aacgaggact acaccgtggt ggaacagtac gaacgctccg agggccgcca ctccaccggc
      781 ggcatggacg agctgtacaa gtacccatat gatgtcccgg attacgctag ctctggtcca
      841 agtggatcaa gctcggatcc tctagagtcg
//
