LOCUS       P393_pHACK(Gal4)       17212 bp DNA     circular SYN 21-NOV-2022
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  recombinant plasmid
REFERENCE   1  (bases 1 to 17212)
  AUTHORS   Cornell University
  TITLE     Direct Submission
  JOURNAL   Exported Nov 21, 2022 from SnapGene 6.1.2
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..17212
                     /mol_type="other DNA"
                     /organism="recombinant plasmid"
     misc_feature    1..233
                     /label=P element 3' end
                     /note="P element 3' end"
     primer_bind     227..270
                     /label=O994_pAC-Gal4_F
     CDS             246..1364
                     /codon_start=1
                     /gene="Gal4"
                     /label=Gal4
                     /translation="MKLLSSIEQACDICRLKKLKCSKEKPKCAKCLKNNWECRYSPKTK
                     RSPLTRAHLTEVESRLERLEQLFLLIFPREDLDMILKMDSLQDIKALLTGLFVQDNVNK
                     DAVTDRLASVETDMPLTLRQHRISATSSSEESSNKGQRQLTVSIDSAAHHDNSTIPLDF
                     MPRDALHGFDWSEEDDMSDGLPFLKTDPNNNGFFGDGSLLCILRSIGFKPENYTNSNVN
                     RLPTMITDRYTLASRSTTSRLLQSYLNNFHPYCPIVHSPTLMMLYNNQIEIASKDQWQI
                     LFNCILAIGAWCIEGESTDIDVFYYQNAKSHLTSKVFESGSIILVTALHLLSRYTQWRQ
                     KTNTSYNFHSFSIRMAISLGLNRDLPSSFSDSS"
     CDS             246..686
                     /gene="Saccharomyces cerevisiae GAL4 (truncated)"
                     /product="DNA binding domain of the GAL4 transcriptional 
                     activator"
                     /label=GAL4 DNA binding domain
     primer_bind     complement(1346..1412)
                     /label=O995_T2A-Gal4(5HA)_R
     CDS             1368..1421
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     primer_bind     1423..1458
                     /label=O1000_GS-LexA_F
     CDS             1440..2993
                     /codon_start=1
                     /label=nlsLexA-GADfl
                     /note="nlsLexA-GADfl"
                     /translation="MPPKKKRKVEDPMKALTARQQEVFDLIRDHISQTGMPPTRAEIAQ
                     RLGFRSPNAAEEHLKALARKGVIEIVSGASRGIRLLQEEEEGLPLVGRVAAGEPLLAQQ
                     HIEGHYQVDPSLFKPNADFLLRVSGMSMKDIGIMDGDLLAVHKTQDVRNGQVVVARIDD
                     EVTVKRLKKQGNKVELLPENSEFKPIVVDLRQQSFTIEGLAVGVIRNGDWLGSIDSAAH
                     HDNSTIPLDFMPRDALHGFDWSEEDDMSDGLPFLKTDPNNNGFFGDGSLLCILRSIGFK
                     PENYTNSNVNRLPTMITDRYTLASRSTTSRLLQSYLNNFHPYCPIVHSPTLMMLYNNQI
                     EIASKDQWQILFNCILAIGAWCIEGESTDIDVFYYQNAKSHLTSKVFESGSIILVTALH
                     LLSRYTQWRQKTNTSYNFHSFSIRMAISLGLNRDLPSSFSDSSILEQRRRIWWSVYSWE
                     IQLSLLYGRSIQLSQNTISFPSSVDDVQRTTTGPTIYHGIIETARLLQVFTKIYELDKT
                     V"
     CDS             1446..1466
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
     primer_bind     complement(2976..3018)
                     /label=O1001_GAD_R
     CDS             2994..4187
                     /codon_start=1
                     /gene="Gal4"
                     /label=Gal4
                     /translation="TAEKSPICAKKCLMICNEIEEVSRQAPKFLQMDISTTALTNLLKE
                     HPWLSFTRFELKWKQLSLIIYVLRDFFTNFTQKKSQLEQDQNDHQSYEVKRCSIMLSDA
                     AQRTVMSVSSYMDNHNVTPYFAWNCSYYLFNAVLVPIKTLLSNSKSNAENNETAQLLQQ
                     INTVLMLLKKLATFKIQTCEKYIQVLEEVCAPFLLSQCAIPLPHISYNNSNGSAIKNIV
                     GSATIAQYPTLPEENVNNISVKYVSPGSVGPSPVPLKSGASFSDLVKLLSNRPPSRNSP
                     VTIPRSTPSHRSVTPFLGQQQQLQSLVPLTPSALFGGANFNQSGNIADSSLSFTFTNSS
                     NGPNLITTQTNSQALSQPIASSNVHDNFMNNEITASKIDDGNNSKPLSPGWTDQTAYN"
     primer_bind     complement(4166..4206)
                     /label=O999_pAC-Gal4_R
     promoter        4215..5342
                     /gene="CR34335"
                     /label=CR7T promoter
     primer_bind     complement(4276..4296)
                     /label=O738_CR7T_5R
     primer_bind     4978..5012
                     /label=O737_CR7T_HiTm_F
     primer_bind     5217..5239
                     /label=O736_CR7T_seqF
     misc_feature    5343..5362
                     /label=Gal4-TS1
     misc_feature    5363..5458
                     /label=gRNA core 2.1
     terminator      5459..5467
                     /label=U6:2 terminator
                     /note="U6:96Ab terminator"
     promoter        5460..5880
                     /label=dU6-3 promoter
                     /note="RNA polymerase III promoter for Drosophila U6-3 
                     snRNA (Port et al., 2014)"
     misc_feature    5881..5900
                     /label=Gal4-TS2
     misc_feature    5901..5996
                     /label=gRNA core 2.1
     misc_feature    5997..6112
                     /label=U6 genes 3' flanking sequence
                     /note="U6 genes 3' flanking sequence"
     enhancer        6119..7932
                     /label=Poly-Ubiquitin promoter
                     /note="Promoter of Ubi-p63E; upper case indicate 5'UTR 
                     (alternative first exon)"
     misc_signal     7950..7970
                     /label=Syn21
                     /note="Syn21 translational enhancer"
     CDS             7971..7982
                     /codon_start=1
                     /label=Start
                     /translation="MSGT"
     primer_bind     7992..8007
                     /label=O1000_GS-LexA_F
     CDS             7995..8015
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             8028..8057
                     /codon_start=1
                     /label=GS linker
                     /note="GS linker"
                     /translation="GGGGSGGGGS"
     CDS             8058..8768
                     /codon_start=1
                     /label=mTagBFP
                     /note="mTagBFP, Drosophila codon optimized"
                     /translation="MVSKGEELIKENMHMKLYMEGTVDNHHFKCTSEGEGKPYEGTQTM
                     RIKVVEGGPLPFAFDILATSFLYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGG
                     VLTATQDTSLQDGCLIYNVKIRGVNFTSNGPVMQKKTLGWEAFTETLYPADGGLEGRND
                     MALKLVGGSHLIANAKTTYRSKKPAKNLKMPGVYYVDYRLERIKEANNETYVEQHEVAV
                     ARYCDLPSKLGHKLN"
     primer_bind     8058..8077
                     /label=O920_TagBFP2_F
     primer_bind     complement(8751..8800)
                     /label=O921_His-TagBFP2_R
     misc_feature    8787..9427
                     /label=His2Av
     3'UTR           8791..9113
                     /label=3'UTR of His2Av
                     /note="3'UTR of His2Av"
     polyA_signal    9077..9082
                     /label=polyA
                     /note="polyA signal predicted by Softberry-FGENESH"
     CDS             join(9922..9993,10388..10661,10736..11390,11452..11767,
                     11988..12119,12190..12804)
                     /codon_start=1
                     /gene="white"
                     /product="Drosophila white gene eye color pigment"
                     /label=mini-white
                     /note="This is a modified version of the white gene lacking
                     part of the first intron."
                     /translation="MGQEDQELLIRGGSKHPSAEHLNNGDSGAASQSCINQGFGQAKNY
                     GTLLPPSPPEDSGSGSGQLAENLTYAWHNMDIFGAVNQPGSGWRQLVNRTRGLFCNERH
                     IPAPRKHLLKNVCGVAYPGELLAVMGSSGAGKTTLLNALAFRSPQGIQVSPSGMRLLNG
                     QPVDAKEMQARCAYVQQDDLFIGSLTAREHLIFQAMVRMPRHLTYRQRVARVDQVIQEL
                     SLSKCQHTIIGVPGRVKGLSGGERKRLAFASEALTDPPLLICDEPTSGLDSFTAHSVVQ
                     VLKKLSQKGKTVILTIHQPSSELFELFDKILLMAEGRVAFLGTPSEAVDFFSYVGAQCP
                     TNYNPADFYVQVLAVVPGREIESRDRIAKICDNFAISKVARDMEQLLATKNLEKPLEQP
                     ENGYTYKATWFMQFRAVLWRSWLSVLKEPLLVKVRLIQTTMVAILIGLIFLGQQLTQVG
                     VMNINGAIFLFLTNMTFQNVFATINVFTSELPVFMREARSRLYRCDTYFLGKTIAELPL
                     FLTVPLVFTAIAYPMIGLRAGVLHFFNCLALVTLVANVSTSFGYLISCASSSTSMALSV
                     GPPVIIPFLLFGGFFLNSGSVPVYLKWLSYLSWFRYANEGLLINQWADVEPGEISCTSS
                     NTTCPSSGKVILETLNFSAADLPLDYVGLAILIVSFRVLAYLALRLRARRKE"
     misc_feature    13582..14167
                     /label=P element 5' end
     misc_feature    14168..14395
                     /label=w linker
                     /note="w[+]car6.1_5', partial sequence of w last exon, 
                     inversed"
     rep_origin      complement(14402..14990)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(15161..16021)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(16022..16126)
                     /gene="bla"
                     /label=AmpR promoter
     protein_bind    16601..16670
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="attB site for the phi-C31 integrase (Groth et al., 
                     2000)"
     misc_feature    16711..17212
                     /label=w linker
                     /note="w[+]car6.1_3', patial seqence of w last exon and 3 
                     'UTR"
ORIGIN
        1 catgatgaaa taacataagg tggtcccgtc ggcaagagac atccacttaa cgtatgcttg
       61 caataagtgc gagtgaaagg aatagtattc tgagtgtcgt attgagtctg agtgagacag
      121 cgatatgatt gttgattaac ccttagcatg tccgtggggt ttgaattaac tcataatatt
      181 aattagacga aattattttt aaagttttat ttttaataat ttgcgagtac gcaaagcttg
      241 gctgcatgaa gctactgtct tctatcgaac aagcatgcga tatttgccga cttaaaaagc
      301 tcaagtgctc caaagaaaaa ccgaagtgcg ccaagtgtct gaagaacaac tgggagtgtc
      361 gctactctcc caaaaccaaa aggtctccgc tgactagggc acatctgaca gaagtggaat
      421 caaggctaga aagactggaa cagctatttc tactgatttt tcctcgagaa gaccttgaca
      481 tgattttgaa aatggattct ttacaggata taaaagcatt gttaacagga ttatttgtac
      541 aagataatgt gaataaagat gccgtcacag atagattggc ttcagtggag actgatatgc
      601 ctctaacatt gagacagcat agaataagtg cgacatcatc atcggaagag agtagtaaca
      661 aaggtcaaag acagttgact gtatcgattg actcggcagc tcatcatgat aactccacaa
      721 ttccgttgga ttttatgccc agggatgctc ttcatggatt tgattggtct gaagaggatg
      781 acatgtcgga tggcttgccc ttcctgaaaa cggaccccaa caataatggg ttctttggcg
      841 acggttctct cttatgtatt cttcgatcta ttggctttaa accggaaaat tacacgaact
      901 ctaacgttaa caggctcccg accatgatta cggatagata cacgttggct tctagatcca
      961 caacatcccg tttacttcaa agttatctca ataattttca cccctactgc cctatcgtgc
     1021 actcaccgac gctaatgatg ttgtataata accagattga aatcgcgtcg aaggatcaat
     1081 ggcaaatcct ttttaactgc atattagcca ttggagcctg gtgtatagag ggggaatcta
     1141 ctgatataga tgttttttac tatcaaaatg ctaaatctca tttgacgagc aaggtcttcg
     1201 agtcaggttc cataattttg gtgacagccc tacatcttct gtcgcgatat acacagtgga
     1261 ggcagaaaac aaatactagc tataattttc acagcttttc cataagaatg gccatatcat
     1321 tgggcttgaa tagggacctc ccctcgtcct tcagtgatag cagcggagag ggcagaggaa
     1381 gtctgctaac atgcggtgac gtcgaggaga atcctggccc aggaagcgga ggcgctagca
     1441 tgccacccaa gaagaagcga aaagtagaag atccaatgaa ggctctcacg gcccgacaac
     1501 aggaagtttt tgatttgata cgggatcata tatcccaaac gggtatgcct ccgacccgcg
     1561 cagagatagc acagcgactg ggctttcgat cgcctaacgc cgcggaggag cacttgaagg
     1621 cactggcccg caagggtgtc attgaaatcg tgtccggtgc gagccgcgga atccggctgt
     1681 tgcaggagga ggaagagggc ctgccactgg tgggacgcgt ggccgctggc gagccgctgc
     1741 tggcccagca acacatagag ggacactatc aggtggaccc ctccttgttt aagccaaatg
     1801 ctgatttcct gttgcgggtg tcgggaatgt ccatgaagga catcggtatt atggatggtg
     1861 acctcctcgc cgtccataag acacaggatg tcaggaacgg ccaggtagtc gttgccagga
     1921 tagacgatga ggtcactgtg aaacgtctca agaagcaagg caataaggtc gagctgctgc
     1981 cggagaatag cgagttcaag ccgatcgtgg tggatctgcg acagcagtcc tttactatcg
     2041 agggcttggc cgtgggtgtg atccgcaacg gagattggct gggatccatc gacagtgcgg
     2101 cccaccacga caacagcacc attccactgg acttcatgcc tcgtgacgca ctgcatggat
     2161 ttgactggag cgaggaggat gatatgagcg acggactccc attcctgaag accgacccca
     2221 ataacaatgg tttctttggc gacggcagcc tgctgtgtat cctgcgaagt attggtttta
     2281 agccggaaaa ttacacgaac agtaatgtca atcgtcttcc cacaatgatc accgaccgtt
     2341 acacgctggc cagtcgcagt actacaagcc gcctgctgca aagctatctt aacaacttcc
     2401 acccctactg ccccatagtg catagtccta ctctcatgat gctttacaac aatcagattg
     2461 agatagcatc gaaggaccag tggcagatcc tcttcaactg cattctcgcc atcggcgctt
     2521 ggtgtattga aggcgagagc acagatatcg acgtgtttta ctatcaaaac gccaagtcgc
     2581 acctcacctc gaaggtcttc gaaagtggaa gcattatcct cgtgacagcc ctccatctgc
     2641 tgagtcgtta tacccagtgg cgtcagaaaa ctaatacatc ctacaacttt cattcgttca
     2701 gcattcggat ggcgattagc ctgggactta atcgtgacct cccaagtagc ttcagcgatt
     2761 cgtcgatcct ggaacagcgc cggaggattt ggtggagtgt gtacagctgg gagatccagc
     2821 tgtccctctt gtacggacgc agcattcagc tgagtcagaa cacgatttcc ttcccgtcca
     2881 gcgtggacga cgttcaacgg acaactacgg gcccgaccat ctaccacggt atcattgaga
     2941 cggcccgctt gcttcaagtg tttacgaaga tttatgagct ggataagaca gtgactgcag
     3001 aaaaaagtcc tatatgtgca aaaaaatgct tgatgatttg taatgagatt gaggaggttt
     3061 cgagacaggc accaaagttt ttacaaatgg atatttccac caccgctcta accaatttgt
     3121 tgaaggaaca cccttggcta tcctttacaa gattcgaact gaagtggaaa cagttgtctc
     3181 ttatcattta tgtattaaga gattttttca ctaattttac ccagaaaaag tcacaactag
     3241 aacaggatca aaatgatcat caaagttatg aagttaaacg atgctccatc atgttaagcg
     3301 atgcagcaca aagaactgtt atgtctgtaa gtagctatat ggacaatcat aatgtcaccc
     3361 catattttgc ctggaattgt tcttattact tgttcaatgc agtcctagta cccataaaga
     3421 ctctactctc aaactcaaaa tcgaatgctg agaataacga gaccgcacaa ttattacaac
     3481 aaattaacac tgttctgatg ctattaaaaa aactggccac ttttaaaatc cagacttgtg
     3541 aaaaatacat tcaagtactg gaagaggtat gtgcgccgtt tctgttatca cagtgtgcaa
     3601 tcccattacc gcatatcagt tataacaata gtaatggtag cgccattaaa aatattgtcg
     3661 gttctgcaac tatcgcccaa taccctactc ttccggagga aaatgtcaac aatatcagtg
     3721 ttaaatatgt ttctcctggc tcagtagggc cttcacctgt gccattgaaa tcaggagcaa
     3781 gtttcagtga tctagtcaag ctgttatcta accgtccacc ctctcgtaac tctccagtga
     3841 caataccaag aagcacacct tcgcatcgct cagtcacgcc ttttctaggg caacagcaac
     3901 agctgcaatc attagtgcca ctgaccccgt ctgctttgtt tggtggcgcc aattttaatc
     3961 aaagtgggaa tattgctgat agctcattgt ccttcacttt cactaacagt agcaacggtc
     4021 cgaacctcat aacaactcaa acaaattctc aagcgctttc acaaccaatt gcctcctcta
     4081 acgttcatga taacttcatg aataatgaaa tcacggctag taaaattgat gatggtaata
     4141 attcaaaacc actgtcacct ggttggacgg accaaactgc gtataacaaa gtgaacacgt
     4201 cgctaagcga aagcccgttt tgtcatcgct ttttgtcggg tctcagttgt ggatcgaaaa
     4261 cccggagagt aaccccttga ccccttgtca gtttccgcat gctccagccc ttgtcctcat
     4321 attttccaat cgtttttggc gtttttagct gcggccattg aatgttcaaa aattattgta
     4381 aacttttcag accccctccg tgtgtgtgtg tgtgtgtgtg cttctgtgta tgtggaaaag
     4441 ttcgaattct agttgggaaa tcaattaaaa gcgcttgttg gactcgcacc acaactttcc
     4501 ctttcgatcg cccctgcggc ttggttcaac tggtcaaggc ctctagctcc cgaaaaccgt
     4561 cgaaaattgg tttggcactt tttatagcca cccaatataa taccaagttc agacagaaat
     4621 tgatatgggt attggactga tcaataactt aagttccctc actgaattat ccttaactct
     4681 tgtgcatgcc tatttatgtg aaaacaatca aaattactca attatgtatg cacaggtatt
     4741 gaaagctccc actatttagt ttttgtttta attatctgtc tttagaactt taaatggaaa
     4801 aacatgactt atatgtatga acaaaaattc caatcgatca ctttaggcgg ccgccaatgc
     4861 caacagaagc attatttttt tcctttcccg atctctcaag cacactcagt tgatctcagt
     4921 cgcaaaactc gttactcact cttaatattt cctcttcttt gcactttcct ggtggaatgc
     4981 attctctatt ttcctctccc tactgcacat actctttgag ctctctagca gataccctaa
     5041 caatttctaa tcatccacga ataataaaca aagatttcta atatcttaaa cctgttttat
     5101 atacttatta ttcaaaaaaa aaagtgtggc aaaaaaaaag agcacttcaa cgtttgttga
     5161 acaaaggata taattttgta agggtatcta gaagttttca tgcctctctt catccactct
     5221 tctcaacctc atgcgatagc tgctgcgctc tctcgtttat cgttcggttg aggttttata
     5281 attctcaaat actttttccc gaagtggtgc ctttaaatag cgtatatgag tggaagactt
     5341 tcgatgtgca gcgtaccaca acgtttaaga gctatgctgg aaacagcata gcaagtttaa
     5401 ataaggctag tccgttatca actttgctgg aaacagcaaa gtggcaccga gtcggtgctt
     5461 tttttgctca cctgtgattg ctcctactca aatacaaaaa catcaaattt tctgtcaata
     5521 aagcatattt atttatattt attttacagg aaagaattcc ttttaaagtg tattttaacc
     5581 tataatgaaa aacgattaaa aaaaatacat aaaataattc gaaaattttt gaatagccca
     5641 ggttgataaa aattcatttc atacgtttta taacttatgc ccctaagtat tttttgacca
     5701 tagtgtttca attctacatt aattttacag agtagaatga aacgccacct actcagccaa
     5761 gaggcgaaaa ggttagctcg ccaagcagag agggcgccag tgctcactac tttttataat
     5821 tctcaacttc tttttccaga ctcagttcgt atatatagac ctattttcaa tttaacgtcg
     5881 tgtattctga gaaagctgga gtttaagagc tatgctggaa acagcatagc aagtttaaat
     5941 aaggctagtc cgttatcaac tttgctggaa acagcaaagt ggcaccgagt cggtgctttt
     6001 tttgcctacc tggagcctga gagttgttca ataaaataaa aatgtttcgt ttttttgctt
     6061 tcgccagtat ttattatttt tcatcaatat gtattcaatt tggtatgtat ttacgcgtgg
     6121 cttgctgttc ttcgcgttca aaatctccag ctccattttg ctttcggtgc gcttgcaatc
     6181 agtactgtcc aaaatcgaaa atcgccgaac cgtagtgtga ccgtgcgggg ctctgcgaaa
     6241 ataaactttt ttaggtatat ggccacacac ggggaaagca cagtggatta tatgtattaa
     6301 tatattatgc aggttttcat tacttatcca gatgtaagcc cacttaaagc gatttaacaa
     6361 ttatttgccg aaagagtata aacaaatttc acataaaaat ggattaagaa aagcttgtgt
     6421 aagattatgc gcagcgttgc cagatagctc catttaaaac acttcaaaaa caataagttt
     6481 agaaaatata tacataaata gcagtcgttg ccgcaacgct caacacatca cacttttaaa
     6541 acacccttta cctacacaga attacttttt aaatttccag tcaagctgcg agtttcaaaa
     6601 ttatagccgg tagagaagac agtgctattt caaaagcaaa ctaacaaggg tcttaaattt
     6661 caaaacacca atcctaacaa gccttggact tttgtaagtt tagatcaaag gtggcattgc
     6721 attcaatgtc atggtaagaa gtaggtcgtc taggtagaaa tcctcattca gccggtcaag
     6781 tcagtacgag aaaggtctca atttgaaatt gtcttaaaaa tattttattg ttttgtactg
     6841 tggtgagttt aaacgaaaaa cacaaaaaaa aagtgataca cagaaatcat aaaaaatttt
     6901 aatacaaggt attcgtacgt atcaaaaaca tttcggcaca attttttttc tctgtactaa
     6961 agtgttacga acactacggt attttttagt gattttcaac ggacaccgaa ggtatataaa
     7021 cagcgttcgc gaacggtcgc cttcaaaacc aattgacatt tgcagcagca agtacaagta
     7081 gaaagtaaag cgcaatcagc gaaaaattta tacttaattg ttggtgatta aagtacaatt
     7141 aaaagaacat tctcgaaagt cacaagaaac gtaagttttt aactcgctgt taccaattag
     7201 taataagagc aacaagacgt tgagtaattt caagaaaaac tgcatttcaa ggtctttgtt
     7261 cggccatttt ttttttattc aacgctctac gtaattacaa aataagaaat tggcagccac
     7321 gcatcttgtt ttcccaatga attggcatca aaacgcaaac aaatctataa ataaaacttg
     7381 cgtgttgatt ttcgccaaga tttattggca aattgtgaaa ttcgcagtga cgcatttgaa
     7441 aattcgagaa atcacgaacg cactcgagca tttgtgtgca tgttattagt tagttgttta
     7501 gttaattgaa gtattttacc aacgaaatcc acttattttt agctgaaata gagtaggttg
     7561 cttaaacaaa gccacgtctg aaaatttctt attgcttgta gttgtgacgt caccatatac
     7621 acacaaaata atgtgtatgc atgcgtttca gctgtgtata tatacatgca cacactcgca
     7681 ttatgaaaac gatgacgagc aacggaacag gtttctcaac tacctttgtt cctgtttctt
     7741 cgctttcctt tgttccaata ttcgtagagg gttaataggg gtttctcaac aaagttggcg
     7801 tcgataaata agtttcccat ttttattccc cagccaggaa gttagtttca atagttttgt
     7861 aatttcaacg aaactcattt gatttcgtac taattttcca catctctatt ttctgcccgc
     7921 agaataatcc aactgaatag ggaattggga acttaaaaaa aaaaatcaaa atgagcggta
     7981 ccgatcgcag cccccccaag aagaagcgca aggtggagtt cggatccggt ggcggcggaa
     8041 gtggaggtgg aggctcgatg gtgtccaagg gcgaggagct gatcaaggag aacatgcaca
     8101 tgaagctgta catggagggc accgtggaca accaccactt caagtgcacc tccgagggcg
     8161 agggcaagcc ctacgagggc acccagacca tgcgcatcaa ggtggtggag ggcggccccc
     8221 tgcccttcgc cttcgacatc ctggccacct ccttcctgta cggctccaag accttcatca
     8281 accacaccca gggcatcccc gacttcttca agcagtcctt ccccgagggc ttcacctggg
     8341 agcgcgtgac cacctacgag gacggcggcg tgctgaccgc cacccaggac acctccctgc
     8401 aagacggctg cctgatctac aacgtgaaga tccgcggcgt gaacttcacc tccaacggcc
     8461 ccgtgatgca gaagaagacc ctgggctggg aggccttcac cgagaccctg taccccgccg
     8521 acggcggcct ggagggccgc aacgacatgg ccctgaagct ggtgggcggc tcccacctga
     8581 tcgccaacgc caagaccacc taccgctcca agaagcccgc caagaacctg aagatgcccg
     8641 gcgtgtacta cgtggactac cgcctggagc gcatcaagga ggccaacaac gagacctacg
     8701 tggagcagca cgaggtggcc gtggcccgct actgcgacct gccctccaag ctgggccaca
     8761 agctgaacta atctagaatt aattaactaa gccagtcggc aatcggacgc cttcgaaaca
     8821 tgcaacacta atgtttaatt cagatttcag cagagacaag ctaaaacacc gacgagttgt
     8881 aatcatttct gtgcgccagc atatatttct tatatacaac gtaatacata attatgtaat
     8941 tctagcatct ccccaacact cacatacaaa caaacaaaaa atacaaacac acaaaacgta
     9001 tttacccgca cgcatccttg gcgaggttga gtatgaaaca aaaacaaaac ttaatttaga
     9061 gcaaagtaat tacacgaata aatttaataa aaaaaaacta taataaaaac gccaatcatg
     9121 ttatttccaa aaaaaaaaaa aaaaaaacta gacgagatgt ttgcgcttgt gtcgatgaat
     9181 gtaaagaatt gcaactacat agatggtaat taatttcagc tgtaagaagt acaagttttt
     9241 cataattgtg agaaaagtgc agtagtatcg cttaaagttc agttttaatc agggcattta
     9301 gcagctcgaa cccaatgtat ttgtaagccc aataaacgtg taaatgccta gagcaaaata
     9361 tcacaactgt agcttttcat ccgaaagata ataatgaata gactgacctg acgtttttca
     9421 aaatgcgact agtggcctaa ttctagtatg tatgtaagtt aataaaaccc atttttgcgg
     9481 aaagtagata aaaaaaacat tttttttttt tactgcactg gatatcattg aacttatctg
     9541 atcagtttta aatttacttc gatccaaggg tatttgatgt accaggttct ttcgattacc
     9601 tctcactcaa aatgacattc cactcaaagt cagcgctgtt tgcctccttc tctgtccaca
     9661 gaaatatcgc cgtctctttc gccgctgcgt ccgctatctc tttcgccacc gtttgtagcg
     9721 ttacgtagcg tcaatgtccg ccttcagttg cattttgtca gcggtttcgt gacgaagctc
     9781 caagcggttt acgccatcaa ttaaacacaa agtgctgtgc caaaactcct ctcgcttctt
     9841 atttttgttt gttttttgag tgattggggt ggtgattggt tttgggtggg taagcagggg
     9901 aaagtgtgaa aaatcccggc aatgggccaa gaggatcagg agctattaat tcgcggaggc
     9961 agcaaacacc catctgccga gcatctgaac aatgtgagta gtacatgtgc atacatctta
    10021 agttcacttg atctatagga actgcgattg caacatcaaa ttgtctgcgg cgtgagaact
    10081 gcgacccaca aaaatcccaa accgcaatcg cacaaacaaa tagtgacacg aaacagatta
    10141 ttctggtagc tgtgctcgct atataagaca atttttaaga tcatatcatg atcaagacat
    10201 ctaaaggcat tcattttcga ctacattctt ttttacaaaa aatataacaa ccagatattt
    10261 taagctgatc ctagatgcac aaaaaataaa taaaagtata aacctacttc gtaggatact
    10321 tcgttttgtt cggggttaga tgagcataac gcttgtagtt gatatttgag atcccctatc
    10381 attgcagggt gacagcggag cggcttcgca gagctgcatt aaccagggct tcgggcaggc
    10441 caaaaactac ggcacgctcc tgccacccag tccgccggag gactccggtt cagggagcgg
    10501 ccaactagcc gagaacctca cctatgcctg gcacaatatg gacatctttg gggcggtcaa
    10561 tcagccgggc tccggatggc ggcagctggt caaccggaca cgcggactat tctgcaacga
    10621 gcgacacata ccggcgccca ggaaacattt gctcaagaac ggtgagtttc tattcgcagt
    10681 cggctgatct gtgtgaaatc ttaataaagg gtccaattac caatttgaaa ctcagtttgc
    10741 ggcgtggcct atccgggcga acttttggcc gtgatgggca gttccggtgc cggaaagacg
    10801 accctgctga atgcccttgc ctttcgatcg ccgcagggca tccaagtatc gccatccggg
    10861 atgcgactgc tcaatggcca acctgtggac gccaaggaga tgcaggccag gtgcgcctat
    10921 gtccagcagg atgacctctt tatcggctcc ctaacggcca gggaacacct gattttccag
    10981 gccatggtgc ggatgccacg acatctgacc tatcggcagc gagtggcccg cgtggatcag
    11041 gtgatccagg agctttcgct cagcaaatgt cagcacacga tcatcggtgt gcccggcagg
    11101 gtgaaaggtc tgtccggcgg agaaaggaag cgtctggcat tcgcctccga ggcactaacc
    11161 gatccgccgc ttctgatctg cgatgagccc acctccggac tggactcatt taccgcccac
    11221 agcgtcgtcc aggtgctgaa gaagctgtcg cagaagggca agaccgtcat cctgaccatt
    11281 catcagccgt cttccgagct gtttgagctc tttgacaaga tccttctgat ggccgagggc
    11341 agggtagctt tcttgggcac tcccagcgaa gccgtcgact tcttttccta gtgagttcga
    11401 tgtgtttatt aagggtatct agcattacat tacatctcaa ctcctatcca gcgtgggtgc
    11461 ccagtgtcct accaactaca atccggcgga cttttacgta caggtgttgg ccgttgtgcc
    11521 cggacgggag atcgagtccc gtgatcggat cgccaagata tgcgacaatt ttgctattag
    11581 caaagtagcc cgggatatgg agcagttgtt ggccaccaaa aatttggaga agccactgga
    11641 gcagccggag aatgggtaca cctacaaggc cacctggttc atgcagttcc gggcggtcct
    11701 gtggcgatcc tggctgtcgg tgctcaagga accactcctc gtaaaagtgc gacttattca
    11761 gacaacggtg agtggttcca gtggaaacaa atgatataac gcttacaatt cttggaaaca
    11821 aattcgctag attttagtta gaattgcctg attccacacc cttcttagtt tttttcaatg
    11881 agatgtatag tttatagttt tgcagaaaat aaataaattt catttaactc gcgaacatgt
    11941 tgaagatatg aatattaatg agatgcgagt aacattttaa tttgcagatg gttgccatct
    12001 tgattggcct catctttttg ggccaacaac tcacgcaagt gggcgtgatg aatatcaacg
    12061 gagccatctt cctcttcctg accaacatga cctttcaaaa cgtctttgcc acgataaatg
    12121 taagtcttgt ttagaataca tttgcatatt aataatttac taactttcta atgaatcgat
    12181 tcgatttagg tgttcacctc agagctgcca gtttttatga gggaggcccg aagtcgactt
    12241 tatcgctgtg acacatactt tctgggcaaa acgattgccg aattaccgct ttttctcaca
    12301 gtgccactgg tcttcacggc gattgcctat ccgatgatcg gactgcgggc cggagtgctg
    12361 cacttcttca actgcctggc gctggtcact ctggtggcca atgtgtcaac gtccttcgga
    12421 tatctaatat cctgcgccag ctcctcgacc tcgatggcgc tgtctgtggg tccgccggtt
    12481 atcataccat tcctgctctt tggcggcttc ttcttgaact cgggctcggt gccagtatac
    12541 ctcaaatggt tgtcgtacct ctcatggttc cgttacgcca acgagggtct gctgattaac
    12601 caatgggcgg acgtggagcc gggcgaaatt agctgcacat cgtcgaacac cacgtgcccc
    12661 agttcgggca aggtcatcct ggagacgctt aacttctccg ccgccgatct gccgctggac
    12721 tacgtgggtc tggccattct catcgtgagc ttccgggtgc tcgcatatct ggctctaaga
    12781 cttcgggccc gacgcaagga gtagccgaca tatatccgaa ataactgctt gttttttttt
    12841 ttaccattat taccatcgtg tttactgttt attgccccct caaaaagcta atgtaattat
    12901 atttgtgcca ataaaaacaa gatatgacct atagaataca agtatttccc cttcgaacat
    12961 ccccacaagt agactttgga tttgtcttct aaccaaaaga cttacacacc tgcatacctt
    13021 acatcaaaaa ctcgtttatc gctacataaa acaccgggat atatttttta tatacatact
    13081 tttcaaatcg cgcgccctct tcataattca cctccaccac accacgtttc gtagttgctc
    13141 tttcgctgtc tcccacccgc tctccgcaac acattcacct tttgttcgac gaccttggag
    13201 cgactgtcgt tagttccgcg cgattcggtt cgctcaaatg gttccgagtg gttcatttcg
    13261 tctcaataga aattagtaat aaatatttgt atgtacaatt tatttgctcc aatatatttg
    13321 tatatatttc cctcacagct atatttattc taatttaata ttatgacttt ttaaggtaat
    13381 tttttgtgac ctgttcggag tgattagcgt tacaatttga actgaaagtg acatccagtg
    13441 tttgttcctt gtgtagatgc atctcaaaaa aatggtgggc ataatagtgt tgtttatata
    13501 tatcaaaaat aacaactata ataataagaa tacatttaat ttagaaaatg cttggatttc
    13561 actggaacta gaattaattc ggctgctgct ctaaacgacg catttcgtac tccaaagtac
    13621 gaattttttc cctcaagctc ttattttcat taaacaatga acaggaccta acgcacagtc
    13681 acgttattgt ttacataaat gatttttttt actattcaaa cttactctgt ttgtgtactc
    13741 ccactggtat agccttcttt tatcttttct ggttcaggct ctatcacttt actaggtacg
    13801 gcatctgcgt tgagtcgcct ccttttaaat gtctgacctt ttgcaggtgc agccttccac
    13861 tgcgaatcat taaagtgggt atcacaaatt tgggagtttt caccaaggct gcacccaagg
    13921 ctctgctccc acaattttct cttaatagca cacttcggca cgtgaattaa ttttactcca
    13981 gtcacagctt tgcagcaaaa tttgcaatat ttcatttttt tttattccac gtaagggtta
    14041 atgttttcaa aaaaaaattc gtccgcacac aacctttcct ctcaacaagc aaacgtgcac
    14101 tgaatttaag tgtatacttc ggtaagcttc ggctatcgac gggaccacct tatgttattt
    14161 catcatgggc cagacccacg tagtccagcg gcagatcggc ggcggagaag ttaagcgtct
    14221 ccaggatgac cttgcccgaa ctggggcacg tggtgttcga cgatgtgcag ctaatttcgc
    14281 ccggctccac gtccgcccat tggttaatca gcagaccctc gttggcgtaa cggaaccatg
    14341 agaggtacga caaccatttg aggtatactg gcaccgagcc cgagttcaag aagaaggcgt
    14401 ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt
    14461 ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc
    14521 gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa
    14581 gcgtggcgct ttctcaatgc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct
    14641 ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta
    14701 actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg
    14761 gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc
    14821 ctaactacgg ctacactaga aggacagtat ttggtatctg cgctctgctg aagccagtta
    14881 ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg
    14941 gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt
    15001 tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg
    15061 tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta
    15121 aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg
    15181 aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg
    15241 tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc
    15301 gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg
    15361 agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg
    15421 aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag
    15481 gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat
    15541 caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc
    15601 cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc
    15661 ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa
    15721 ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaacac
    15781 gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt
    15841 cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc
    15901 gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa
    15961 caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca
    16021 tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat
    16081 acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa
    16141 aagtgccacc tgacgtctaa gaaaccatta ttatcatgac attaacctat aaaaataggc
    16201 gtatcacgag gccctttcgt ctcgcgcgtt tcggtgatga cggtgaaaac ctctgacaca
    16261 tgcagctccc ggagacggtc acagcttgtc tgtaagcgga tgccgggagc agacaagccc
    16321 gtcagggcgc gtcagcgggt gttggcgggt gtcggggctg gcttaactat gcggcatcag
    16381 agcagattgt actgagagtg caccatatcg acatgcccgc cgtgaccgtc gagaacccgc
    16441 tgacgctgcc ccgcgtatcc gcacccgccg acgccgtcgc acgtcccgtg ctcaccgtga
    16501 ccaccgcgcc cagcggtttc gagggcgagg gcttcccggt gcgccgcgcg ttcgccggga
    16561 tcaactaccg ccacctcgac ccgttcatca tgatggacca gatgggtgag gtggagtacg
    16621 cgcccgggga gcccaagggc acgccctggc acccgcaccg cggcttcgag accgtgacct
    16681 acatcgtcga tatgcggtgt gaaataccgc accgaatcgc gcggaactaa cgacagtcgc
    16741 tccaaggtcg tcgaacaaaa ggtgaatgtg ttgcggagag cgggtgggag acagcgaaag
    16801 agcaactacg aaacgtggtg tggtggaggt gaattatgaa gagggcgcgc gatttgaaaa
    16861 gtatgtatat aaaaaatata tcccggtgtt ttatgtagcg ataaacgagt ttttgatgta
    16921 aggtatgcag gtgtgtaagt cttttggtta gaagacaaat ccaaagtcta cttgtgggga
    16981 tgttcgaagg ggaaatactt gtattctata ggtcatatct tgtttttatt ggcacaaata
    17041 taattacatt agctttttga gggggcaata aacagtaaac acgatggtaa taatggtaaa
    17101 aaaaaaaaca agcagttatt tcggatatat gtcggctact ccttgcgtcg ggcccgaagt
    17161 cttagagcca gatatgcgag cacccggaag ctcacgatga gaatggccag ac
//
