LOCUS       P394_pHACK(Gal4)       17264 bp DNA     circular SYN 21-NOV-2022
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  recombinant plasmid
REFERENCE   1  (bases 1 to 17264)
  AUTHORS   Cornell University
  TITLE     Direct Submission
  JOURNAL   Exported Nov 21, 2022 from SnapGene 6.1.2
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..17264
                     /mol_type="other DNA"
                     /organism="recombinant plasmid"
     misc_feature    1..233
                     /label=P element 3' end
                     /note="P element 3' end"
     primer_bind     227..270
                     /label=O994_pAC-Gal4_F
     CDS             246..1364
                     /codon_start=1
                     /gene="Gal4"
                     /label=Gal4
                     /translation="MKLLSSIEQACDICRLKKLKCSKEKPKCAKCLKNNWECRYSPKTK
                     RSPLTRAHLTEVESRLERLEQLFLLIFPREDLDMILKMDSLQDIKALLTGLFVQDNVNK
                     DAVTDRLASVETDMPLTLRQHRISATSSSEESSNKGQRQLTVSIDSAAHHDNSTIPLDF
                     MPRDALHGFDWSEEDDMSDGLPFLKTDPNNNGFFGDGSLLCILRSIGFKPENYTNSNVN
                     RLPTMITDRYTLASRSTTSRLLQSYLNNFHPYCPIVHSPTLMMLYNNQIEIASKDQWQI
                     LFNCILAIGAWCIEGESTDIDVFYYQNAKSHLTSKVFESGSIILVTALHLLSRYTQWRQ
                     KTNTSYNFHSFSIRMAISLGLNRDLPSSFSDSS"
     CDS             246..686
                     /gene="Saccharomyces cerevisiae GAL4 (truncated)"
                     /product="DNA binding domain of the GAL4 transcriptional 
                     activator"
                     /label=GAL4 DNA binding domain
     primer_bind     complement(1346..1412)
                     /label=O995_T2A-Gal4(5HA)_R
     CDS             1368..1421
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     primer_bind     1420..1460
                     /label=O1025_GS-Flp_F
     CDS             1440..2711
                     /label=Flippase
                     /note="Flip1 from pJFRC150-20XUAS-IVS-Flp1-PEST, encoding a
                     higher activity Flp with D at AA5."
     primer_bind     complement(2687..2736)
                     /label=O1026_term-Flp_R
     terminator      2716..2972
                     /note="hsp70 terminator"
     CDS             2977..4239
                     /gene="Gal4"
                     /label=Gal4
     primer_bind     2977..2999
                     /label=O998_Gal4(3HA)_F
     primer_bind     complement(4218..4258)
                     /label=O999_pAC-Gal4_R
     promoter        4267..5394
                     /gene="CR34335"
                     /label=CR7T promoter
     primer_bind     complement(4328..4348)
                     /label=O738_CR7T_5R
     primer_bind     5030..5064
                     /label=O737_CR7T_HiTm_F
     primer_bind     5269..5291
                     /label=O736_CR7T_seqF
     misc_feature    5395..5414
                     /label=Gal4-TS1
     misc_feature    5415..5510
                     /label=gRNA core 2.1
     terminator      5511..5519
                     /label=U6:2 terminator
                     /note="U6:96Ab terminator"
     promoter        5512..5932
                     /label=dU6-3 promoter
                     /note="RNA polymerase III promoter for Drosophila U6-3 
                     snRNA (Port et al., 2014)"
     misc_feature    5933..5952
                     /label=Gal4-TS2
     misc_feature    5953..6048
                     /label=gRNA core 2.1
     misc_feature    6049..6164
                     /label=U6 genes 3' flanking sequence
                     /note="U6 genes 3' flanking sequence"
     enhancer        6171..7984
                     /label=Poly-Ubiquitin promoter
                     /note="Promoter of Ubi-p63E; upper case indicate 5'UTR 
                     (alternative first exon)"
     misc_signal     8002..8022
                     /label=Syn21
                     /note="Syn21 translational enhancer"
     CDS             8023..8034
                     /codon_start=1
                     /label=Start
                     /translation="MSGT"
     CDS             8047..8067
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             8080..8109
                     /codon_start=1
                     /label=GS linker
                     /note="GS linker"
                     /translation="GGGGSGGGGS"
     CDS             8110..8820
                     /codon_start=1
                     /label=mTagBFP
                     /note="mTagBFP, Drosophila codon optimized"
                     /translation="MVSKGEELIKENMHMKLYMEGTVDNHHFKCTSEGEGKPYEGTQTM
                     RIKVVEGGPLPFAFDILATSFLYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGG
                     VLTATQDTSLQDGCLIYNVKIRGVNFTSNGPVMQKKTLGWEAFTETLYPADGGLEGRND
                     MALKLVGGSHLIANAKTTYRSKKPAKNLKMPGVYYVDYRLERIKEANNETYVEQHEVAV
                     ARYCDLPSKLGHKLN"
     primer_bind     8110..8129
                     /label=O920_TagBFP2_F
     primer_bind     complement(8803..8852)
                     /label=O921_His-TagBFP2_R
     misc_feature    8839..9479
                     /label=His2Av
     3'UTR           8843..9165
                     /label=3'UTR of His2Av
                     /note="3'UTR of His2Av"
     polyA_signal    9129..9134
                     /label=polyA
                     /note="polyA signal predicted by Softberry-FGENESH"
     CDS             join(9974..10045,10440..10713,10788..11442,11504..11819,
                     12040..12171,12242..12856)
                     /codon_start=1
                     /gene="white"
                     /product="Drosophila white gene eye color pigment"
                     /label=mini-white
                     /note="This is a modified version of the white gene lacking
                     part of the first intron."
                     /translation="MGQEDQELLIRGGSKHPSAEHLNNGDSGAASQSCINQGFGQAKNY
                     GTLLPPSPPEDSGSGSGQLAENLTYAWHNMDIFGAVNQPGSGWRQLVNRTRGLFCNERH
                     IPAPRKHLLKNVCGVAYPGELLAVMGSSGAGKTTLLNALAFRSPQGIQVSPSGMRLLNG
                     QPVDAKEMQARCAYVQQDDLFIGSLTAREHLIFQAMVRMPRHLTYRQRVARVDQVIQEL
                     SLSKCQHTIIGVPGRVKGLSGGERKRLAFASEALTDPPLLICDEPTSGLDSFTAHSVVQ
                     VLKKLSQKGKTVILTIHQPSSELFELFDKILLMAEGRVAFLGTPSEAVDFFSYVGAQCP
                     TNYNPADFYVQVLAVVPGREIESRDRIAKICDNFAISKVARDMEQLLATKNLEKPLEQP
                     ENGYTYKATWFMQFRAVLWRSWLSVLKEPLLVKVRLIQTTMVAILIGLIFLGQQLTQVG
                     VMNINGAIFLFLTNMTFQNVFATINVFTSELPVFMREARSRLYRCDTYFLGKTIAELPL
                     FLTVPLVFTAIAYPMIGLRAGVLHFFNCLALVTLVANVSTSFGYLISCASSSTSMALSV
                     GPPVIIPFLLFGGFFLNSGSVPVYLKWLSYLSWFRYANEGLLINQWADVEPGEISCTSS
                     NTTCPSSGKVILETLNFSAADLPLDYVGLAILIVSFRVLAYLALRLRARRKE"
     misc_feature    13634..14219
                     /label=P element 5' end
     misc_feature    14220..14447
                     /label=w linker
                     /note="w[+]car6.1_5', partial sequence of w last exon, 
                     inversed"
     rep_origin      complement(14454..15042)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(15213..16073)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(16074..16178)
                     /gene="bla"
                     /label=AmpR promoter
     protein_bind    16653..16722
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="attB site for the phi-C31 integrase (Groth et al., 
                     2000)"
     misc_feature    16763..17264
                     /label=w linker
                     /note="w[+]car6.1_3', patial seqence of w last exon and 3 
                     'UTR"
ORIGIN
        1 catgatgaaa taacataagg tggtcccgtc ggcaagagac atccacttaa cgtatgcttg
       61 caataagtgc gagtgaaagg aatagtattc tgagtgtcgt attgagtctg agtgagacag
      121 cgatatgatt gttgattaac ccttagcatg tccgtggggt ttgaattaac tcataatatt
      181 aattagacga aattattttt aaagttttat ttttaataat ttgcgagtac gcaaagcttg
      241 gctgcatgaa gctactgtct tctatcgaac aagcatgcga tatttgccga cttaaaaagc
      301 tcaagtgctc caaagaaaaa ccgaagtgcg ccaagtgtct gaagaacaac tgggagtgtc
      361 gctactctcc caaaaccaaa aggtctccgc tgactagggc acatctgaca gaagtggaat
      421 caaggctaga aagactggaa cagctatttc tactgatttt tcctcgagaa gaccttgaca
      481 tgattttgaa aatggattct ttacaggata taaaagcatt gttaacagga ttatttgtac
      541 aagataatgt gaataaagat gccgtcacag atagattggc ttcagtggag actgatatgc
      601 ctctaacatt gagacagcat agaataagtg cgacatcatc atcggaagag agtagtaaca
      661 aaggtcaaag acagttgact gtatcgattg actcggcagc tcatcatgat aactccacaa
      721 ttccgttgga ttttatgccc agggatgctc ttcatggatt tgattggtct gaagaggatg
      781 acatgtcgga tggcttgccc ttcctgaaaa cggaccccaa caataatggg ttctttggcg
      841 acggttctct cttatgtatt cttcgatcta ttggctttaa accggaaaat tacacgaact
      901 ctaacgttaa caggctcccg accatgatta cggatagata cacgttggct tctagatcca
      961 caacatcccg tttacttcaa agttatctca ataattttca cccctactgc cctatcgtgc
     1021 actcaccgac gctaatgatg ttgtataata accagattga aatcgcgtcg aaggatcaat
     1081 ggcaaatcct ttttaactgc atattagcca ttggagcctg gtgtatagag ggggaatcta
     1141 ctgatataga tgttttttac tatcaaaatg ctaaatctca tttgacgagc aaggtcttcg
     1201 agtcaggttc cataattttg gtgacagccc tacatcttct gtcgcgatat acacagtgga
     1261 ggcagaaaac aaatactagc tataattttc acagcttttc cataagaatg gccatatcat
     1321 tgggcttgaa tagggacctc ccctcgtcct tcagtgatag cagcggagag ggcagaggaa
     1381 gtctgctaac atgcggtgac gtcgaggaga atcctggccc aggaagcgga ggcgctagca
     1441 tgccgcagtt tgatatcctc tgcaagaccc caccaaaggt gttggtgcgt caattcgtgg
     1501 agcgatttga gaggccgtcg ggtgagaaga tcgccctgtg cgctgccgag ttgacctatt
     1561 tgtgttggat gatcactcat aatggcaccg cgattaagcg cgctaccttt atgagctata
     1621 acactatcat tagcaattcc ctgtcctttg acatagtaaa caagtccctg cagtttaaat
     1681 acaagactca gaaggccact atattggagg cttcgctgaa aaagttgatc ccggcatggg
     1741 agttcacgat catcccatac tacggtcaga aacaccagag cgatattacc gatattgtaa
     1801 gcagcctcca gctgcagttt gagtccagcg aagaggctga taagggtaat agtcacagca
     1861 aaaagatgct gaaggcactg ctgtccgagg gcgaaagcat ctgggagatt actgagaaaa
     1921 tcctgaactc gtttgagtac accagccgat tcaccaagac gaagaccctg taccagttcc
     1981 tctttttggc aaccttcatc aattgtggtc gcttcagtga catcaaaaac gtggacccta
     2041 aatcgttcaa gctggtgcag aataagtacc tgggtgttat catccagtgc ttggttaccg
     2101 agactaagac ctccgtgtcc aggcacattt atttcttctc cgcaaggggt cgcatcgatc
     2161 cgctggtata cttggatgag ttccttcgga atagtgaacc agtcttgaag cgcgtgaaca
     2221 ggacgggcaa ttccagtagc aacaagcaag agtaccagct gctgaaggat aatcttgttc
     2281 ggtcgtacaa caaagccttg aagaaaaatg cgccatactc gatctttgca atcaagaatg
     2341 gtcccaagag tcatattggc cggcacctga tgaccagttt cttgtccatg aagggtctga
     2401 ctgagctcac aaacgtcgtt ggcaactggt cggacaagcg agcgagtgcc gtcgcccgca
     2461 cgacatacac ccaccagatc acggccatac cagatcacta tttcgcgctg gtgtcgcgtt
     2521 attatgccta tgatcccatc agcaaggaga tgatcgcgct gaaggacgaa accaatccaa
     2581 tcgaggagtg gcagcatatc gagcaactta agggaagcgc tgagggtagc atccgttacc
     2641 ccgcctggaa cggcatcatc agccaggagg ttctggatta cctgagctcc tacatcaatc
     2701 gccgtattta accggtatcg ataccgtcga ctaaagccaa atagaaaatt attcagttcc
     2761 tggcttaagt ttttaaaagt gatattattt atttggttgt aaccaaccaa aagaatgtaa
     2821 ataactaata cataattatg ttagttttaa gttagcaaca aattgatttt agctatatta
     2881 gctacttggt taataaatag aatatattta tttaaagata attgcgtttt tattgtcagg
     2941 gagtgagttt gcttaaaaac tcgtttagat ccaccgcatg gcatcattga aacagcaagg
     3001 ctcttacaag ttttcacaaa aatctatgaa ctagacaaaa cagtaactgc agaaaaaagt
     3061 cctatatgtg caaaaaaatg cttgatgatt tgtaatgaga ttgaggaggt ttcgagacag
     3121 gcaccaaagt ttttacaaat ggatatttcc accaccgctc taaccaattt gttgaaggaa
     3181 cacccttggc tatcctttac aagattcgaa ctgaagtgga aacagttgtc tcttatcatt
     3241 tatgtattaa gagatttttt cactaatttt acccagaaaa agtcacaact agaacaggat
     3301 caaaatgatc atcaaagtta tgaagttaaa cgatgctcca tcatgttaag cgatgcagca
     3361 caaagaactg ttatgtctgt aagtagctat atggacaatc ataatgtcac cccatatttt
     3421 gcctggaatt gttcttatta cttgttcaat gcagtcctag tacccataaa gactctactc
     3481 tcaaactcaa aatcgaatgc tgagaataac gagaccgcac aattattaca acaaattaac
     3541 actgttctga tgctattaaa aaaactggcc acttttaaaa tccagacttg tgaaaaatac
     3601 attcaagtac tggaagaggt atgtgcgccg tttctgttat cacagtgtgc aatcccatta
     3661 ccgcatatca gttataacaa tagtaatggt agcgccatta aaaatattgt cggttctgca
     3721 actatcgccc aataccctac tcttccggag gaaaatgtca acaatatcag tgttaaatat
     3781 gtttctcctg gctcagtagg gccttcacct gtgccattga aatcaggagc aagtttcagt
     3841 gatctagtca agctgttatc taaccgtcca ccctctcgta actctccagt gacaatacca
     3901 agaagcacac cttcgcatcg ctcagtcacg ccttttctag ggcaacagca acagctgcaa
     3961 tcattagtgc cactgacccc gtctgctttg tttggtggcg ccaattttaa tcaaagtggg
     4021 aatattgctg atagctcatt gtccttcact ttcactaaca gtagcaacgg tccgaacctc
     4081 ataacaactc aaacaaattc tcaagcgctt tcacaaccaa ttgcctcctc taacgttcat
     4141 gataacttca tgaataatga aatcacggct agtaaaattg atgatggtaa taattcaaaa
     4201 ccactgtcac ctggttggac ggaccaaact gcgtataaca aagtgaacac gtcgctaagc
     4261 gaaagcccgt tttgtcatcg ctttttgtcg ggtctcagtt gtggatcgaa aacccggaga
     4321 gtaacccctt gaccccttgt cagtttccgc atgctccagc ccttgtcctc atattttcca
     4381 atcgtttttg gcgtttttag ctgcggccat tgaatgttca aaaattattg taaacttttc
     4441 agaccccctc cgtgtgtgtg tgtgtgtgtg tgcttctgtg tatgtggaaa agttcgaatt
     4501 ctagttggga aatcaattaa aagcgcttgt tggactcgca ccacaacttt ccctttcgat
     4561 cgcccctgcg gcttggttca actggtcaag gcctctagct cccgaaaacc gtcgaaaatt
     4621 ggtttggcac tttttatagc cacccaatat aataccaagt tcagacagaa attgatatgg
     4681 gtattggact gatcaataac ttaagttccc tcactgaatt atccttaact cttgtgcatg
     4741 cctatttatg tgaaaacaat caaaattact caattatgta tgcacaggta ttgaaagctc
     4801 ccactattta gtttttgttt taattatctg tctttagaac tttaaatgga aaaacatgac
     4861 ttatatgtat gaacaaaaat tccaatcgat cactttaggc ggccgccaat gccaacagaa
     4921 gcattatttt tttcctttcc cgatctctca agcacactca gttgatctca gtcgcaaaac
     4981 tcgttactca ctcttaatat ttcctcttct ttgcactttc ctggtggaat gcattctcta
     5041 ttttcctctc cctactgcac atactctttg agctctctag cagataccct aacaatttct
     5101 aatcatccac gaataataaa caaagatttc taatatctta aacctgtttt atatacttat
     5161 tattcaaaaa aaaaagtgtg gcaaaaaaaa agagcacttc aacgtttgtt gaacaaagga
     5221 tataattttg taagggtatc tagaagtttt catgcctctc ttcatccact cttctcaacc
     5281 tcatgcgata gctgctgcgc tctctcgttt atcgttcggt tgaggtttta taattctcaa
     5341 atactttttc ccgaagtggt gcctttaaat agcgtatatg agtggaagac tttcgatgtg
     5401 cagcgtacca caacgtttaa gagctatgct ggaaacagca tagcaagttt aaataaggct
     5461 agtccgttat caactttgct ggaaacagca aagtggcacc gagtcggtgc tttttttgct
     5521 cacctgtgat tgctcctact caaatacaaa aacatcaaat tttctgtcaa taaagcatat
     5581 ttatttatat ttattttaca ggaaagaatt ccttttaaag tgtattttaa cctataatga
     5641 aaaacgatta aaaaaaatac ataaaataat tcgaaaattt ttgaatagcc caggttgata
     5701 aaaattcatt tcatacgttt tataacttat gcccctaagt attttttgac catagtgttt
     5761 caattctaca ttaattttac agagtagaat gaaacgccac ctactcagcc aagaggcgaa
     5821 aaggttagct cgccaagcag agagggcgcc agtgctcact actttttata attctcaact
     5881 tctttttcca gactcagttc gtatatatag acctattttc aatttaacgt cgtgtattct
     5941 gagaaagctg gagtttaaga gctatgctgg aaacagcata gcaagtttaa ataaggctag
     6001 tccgttatca actttgctgg aaacagcaaa gtggcaccga gtcggtgctt tttttgccta
     6061 cctggagcct gagagttgtt caataaaata aaaatgtttc gtttttttgc tttcgccagt
     6121 atttattatt tttcatcaat atgtattcaa tttggtatgt atttacgcgt ggcttgctgt
     6181 tcttcgcgtt caaaatctcc agctccattt tgctttcggt gcgcttgcaa tcagtactgt
     6241 ccaaaatcga aaatcgccga accgtagtgt gaccgtgcgg ggctctgcga aaataaactt
     6301 ttttaggtat atggccacac acggggaaag cacagtggat tatatgtatt aatatattat
     6361 gcaggttttc attacttatc cagatgtaag cccacttaaa gcgatttaac aattatttgc
     6421 cgaaagagta taaacaaatt tcacataaaa atggattaag aaaagcttgt gtaagattat
     6481 gcgcagcgtt gccagatagc tccatttaaa acacttcaaa aacaataagt ttagaaaata
     6541 tatacataaa tagcagtcgt tgccgcaacg ctcaacacat cacactttta aaacaccctt
     6601 tacctacaca gaattacttt ttaaatttcc agtcaagctg cgagtttcaa aattatagcc
     6661 ggtagagaag acagtgctat ttcaaaagca aactaacaag ggtcttaaat ttcaaaacac
     6721 caatcctaac aagccttgga cttttgtaag tttagatcaa aggtggcatt gcattcaatg
     6781 tcatggtaag aagtaggtcg tctaggtaga aatcctcatt cagccggtca agtcagtacg
     6841 agaaaggtct caatttgaaa ttgtcttaaa aatattttat tgttttgtac tgtggtgagt
     6901 ttaaacgaaa aacacaaaaa aaaagtgata cacagaaatc ataaaaaatt ttaatacaag
     6961 gtattcgtac gtatcaaaaa catttcggca caattttttt tctctgtact aaagtgttac
     7021 gaacactacg gtatttttta gtgattttca acggacaccg aaggtatata aacagcgttc
     7081 gcgaacggtc gccttcaaaa ccaattgaca tttgcagcag caagtacaag tagaaagtaa
     7141 agcgcaatca gcgaaaaatt tatacttaat tgttggtgat taaagtacaa ttaaaagaac
     7201 attctcgaaa gtcacaagaa acgtaagttt ttaactcgct gttaccaatt agtaataaga
     7261 gcaacaagac gttgagtaat ttcaagaaaa actgcatttc aaggtctttg ttcggccatt
     7321 ttttttttat tcaacgctct acgtaattac aaaataagaa attggcagcc acgcatcttg
     7381 ttttcccaat gaattggcat caaaacgcaa acaaatctat aaataaaact tgcgtgttga
     7441 ttttcgccaa gatttattgg caaattgtga aattcgcagt gacgcatttg aaaattcgag
     7501 aaatcacgaa cgcactcgag catttgtgtg catgttatta gttagttgtt tagttaattg
     7561 aagtatttta ccaacgaaat ccacttattt ttagctgaaa tagagtaggt tgcttaaaca
     7621 aagccacgtc tgaaaatttc ttattgcttg tagttgtgac gtcaccatat acacacaaaa
     7681 taatgtgtat gcatgcgttt cagctgtgta tatatacatg cacacactcg cattatgaaa
     7741 acgatgacga gcaacggaac aggtttctca actacctttg ttcctgtttc ttcgctttcc
     7801 tttgttccaa tattcgtaga gggttaatag gggtttctca acaaagttgg cgtcgataaa
     7861 taagtttccc atttttattc cccagccagg aagttagttt caatagtttt gtaatttcaa
     7921 cgaaactcat ttgatttcgt actaattttc cacatctcta ttttctgccc gcagaataat
     7981 ccaactgaat agggaattgg gaacttaaaa aaaaaaatca aaatgagcgg taccgatcgc
     8041 agccccccca agaagaagcg caaggtggag ttcggatccg gtggcggcgg aagtggaggt
     8101 ggaggctcga tggtgtccaa gggcgaggag ctgatcaagg agaacatgca catgaagctg
     8161 tacatggagg gcaccgtgga caaccaccac ttcaagtgca cctccgaggg cgagggcaag
     8221 ccctacgagg gcacccagac catgcgcatc aaggtggtgg agggcggccc cctgcccttc
     8281 gccttcgaca tcctggccac ctccttcctg tacggctcca agaccttcat caaccacacc
     8341 cagggcatcc ccgacttctt caagcagtcc ttccccgagg gcttcacctg ggagcgcgtg
     8401 accacctacg aggacggcgg cgtgctgacc gccacccagg acacctccct gcaagacggc
     8461 tgcctgatct acaacgtgaa gatccgcggc gtgaacttca cctccaacgg ccccgtgatg
     8521 cagaagaaga ccctgggctg ggaggccttc accgagaccc tgtaccccgc cgacggcggc
     8581 ctggagggcc gcaacgacat ggccctgaag ctggtgggcg gctcccacct gatcgccaac
     8641 gccaagacca cctaccgctc caagaagccc gccaagaacc tgaagatgcc cggcgtgtac
     8701 tacgtggact accgcctgga gcgcatcaag gaggccaaca acgagaccta cgtggagcag
     8761 cacgaggtgg ccgtggcccg ctactgcgac ctgccctcca agctgggcca caagctgaac
     8821 taatctagaa ttaattaact aagccagtcg gcaatcggac gccttcgaaa catgcaacac
     8881 taatgtttaa ttcagatttc agcagagaca agctaaaaca ccgacgagtt gtaatcattt
     8941 ctgtgcgcca gcatatattt cttatataca acgtaataca taattatgta attctagcat
     9001 ctccccaaca ctcacataca aacaaacaaa aaatacaaac acacaaaacg tatttacccg
     9061 cacgcatcct tggcgaggtt gagtatgaaa caaaaacaaa acttaattta gagcaaagta
     9121 attacacgaa taaatttaat aaaaaaaaac tataataaaa acgccaatca tgttatttcc
     9181 aaaaaaaaaa aaaaaaaaac tagacgagat gtttgcgctt gtgtcgatga atgtaaagaa
     9241 ttgcaactac atagatggta attaatttca gctgtaagaa gtacaagttt ttcataattg
     9301 tgagaaaagt gcagtagtat cgcttaaagt tcagttttaa tcagggcatt tagcagctcg
     9361 aacccaatgt atttgtaagc ccaataaacg tgtaaatgcc tagagcaaaa tatcacaact
     9421 gtagcttttc atccgaaaga taataatgaa tagactgacc tgacgttttt caaaatgcga
     9481 ctagtggcct aattctagta tgtatgtaag ttaataaaac ccatttttgc ggaaagtaga
     9541 taaaaaaaac attttttttt tttactgcac tggatatcat tgaacttatc tgatcagttt
     9601 taaatttact tcgatccaag ggtatttgat gtaccaggtt ctttcgatta cctctcactc
     9661 aaaatgacat tccactcaaa gtcagcgctg tttgcctcct tctctgtcca cagaaatatc
     9721 gccgtctctt tcgccgctgc gtccgctatc tctttcgcca ccgtttgtag cgttacgtag
     9781 cgtcaatgtc cgccttcagt tgcattttgt cagcggtttc gtgacgaagc tccaagcggt
     9841 ttacgccatc aattaaacac aaagtgctgt gccaaaactc ctctcgcttc ttatttttgt
     9901 ttgttttttg agtgattggg gtggtgattg gttttgggtg ggtaagcagg ggaaagtgtg
     9961 aaaaatcccg gcaatgggcc aagaggatca ggagctatta attcgcggag gcagcaaaca
    10021 cccatctgcc gagcatctga acaatgtgag tagtacatgt gcatacatct taagttcact
    10081 tgatctatag gaactgcgat tgcaacatca aattgtctgc ggcgtgagaa ctgcgaccca
    10141 caaaaatccc aaaccgcaat cgcacaaaca aatagtgaca cgaaacagat tattctggta
    10201 gctgtgctcg ctatataaga caatttttaa gatcatatca tgatcaagac atctaaaggc
    10261 attcattttc gactacattc ttttttacaa aaaatataac aaccagatat tttaagctga
    10321 tcctagatgc acaaaaaata aataaaagta taaacctact tcgtaggata cttcgttttg
    10381 ttcggggtta gatgagcata acgcttgtag ttgatatttg agatccccta tcattgcagg
    10441 gtgacagcgg agcggcttcg cagagctgca ttaaccaggg cttcgggcag gccaaaaact
    10501 acggcacgct cctgccaccc agtccgccgg aggactccgg ttcagggagc ggccaactag
    10561 ccgagaacct cacctatgcc tggcacaata tggacatctt tggggcggtc aatcagccgg
    10621 gctccggatg gcggcagctg gtcaaccgga cacgcggact attctgcaac gagcgacaca
    10681 taccggcgcc caggaaacat ttgctcaaga acggtgagtt tctattcgca gtcggctgat
    10741 ctgtgtgaaa tcttaataaa gggtccaatt accaatttga aactcagttt gcggcgtggc
    10801 ctatccgggc gaacttttgg ccgtgatggg cagttccggt gccggaaaga cgaccctgct
    10861 gaatgccctt gcctttcgat cgccgcaggg catccaagta tcgccatccg ggatgcgact
    10921 gctcaatggc caacctgtgg acgccaagga gatgcaggcc aggtgcgcct atgtccagca
    10981 ggatgacctc tttatcggct ccctaacggc cagggaacac ctgattttcc aggccatggt
    11041 gcggatgcca cgacatctga cctatcggca gcgagtggcc cgcgtggatc aggtgatcca
    11101 ggagctttcg ctcagcaaat gtcagcacac gatcatcggt gtgcccggca gggtgaaagg
    11161 tctgtccggc ggagaaagga agcgtctggc attcgcctcc gaggcactaa ccgatccgcc
    11221 gcttctgatc tgcgatgagc ccacctccgg actggactca tttaccgccc acagcgtcgt
    11281 ccaggtgctg aagaagctgt cgcagaaggg caagaccgtc atcctgacca ttcatcagcc
    11341 gtcttccgag ctgtttgagc tctttgacaa gatccttctg atggccgagg gcagggtagc
    11401 tttcttgggc actcccagcg aagccgtcga cttcttttcc tagtgagttc gatgtgttta
    11461 ttaagggtat ctagcattac attacatctc aactcctatc cagcgtgggt gcccagtgtc
    11521 ctaccaacta caatccggcg gacttttacg tacaggtgtt ggccgttgtg cccggacggg
    11581 agatcgagtc ccgtgatcgg atcgccaaga tatgcgacaa ttttgctatt agcaaagtag
    11641 cccgggatat ggagcagttg ttggccacca aaaatttgga gaagccactg gagcagccgg
    11701 agaatgggta cacctacaag gccacctggt tcatgcagtt ccgggcggtc ctgtggcgat
    11761 cctggctgtc ggtgctcaag gaaccactcc tcgtaaaagt gcgacttatt cagacaacgg
    11821 tgagtggttc cagtggaaac aaatgatata acgcttacaa ttcttggaaa caaattcgct
    11881 agattttagt tagaattgcc tgattccaca cccttcttag tttttttcaa tgagatgtat
    11941 agtttatagt tttgcagaaa ataaataaat ttcatttaac tcgcgaacat gttgaagata
    12001 tgaatattaa tgagatgcga gtaacatttt aatttgcaga tggttgccat cttgattggc
    12061 ctcatctttt tgggccaaca actcacgcaa gtgggcgtga tgaatatcaa cggagccatc
    12121 ttcctcttcc tgaccaacat gacctttcaa aacgtctttg ccacgataaa tgtaagtctt
    12181 gtttagaata catttgcata ttaataattt actaactttc taatgaatcg attcgattta
    12241 ggtgttcacc tcagagctgc cagtttttat gagggaggcc cgaagtcgac tttatcgctg
    12301 tgacacatac tttctgggca aaacgattgc cgaattaccg ctttttctca cagtgccact
    12361 ggtcttcacg gcgattgcct atccgatgat cggactgcgg gccggagtgc tgcacttctt
    12421 caactgcctg gcgctggtca ctctggtggc caatgtgtca acgtccttcg gatatctaat
    12481 atcctgcgcc agctcctcga cctcgatggc gctgtctgtg ggtccgccgg ttatcatacc
    12541 attcctgctc tttggcggct tcttcttgaa ctcgggctcg gtgccagtat acctcaaatg
    12601 gttgtcgtac ctctcatggt tccgttacgc caacgagggt ctgctgatta accaatgggc
    12661 ggacgtggag ccgggcgaaa ttagctgcac atcgtcgaac accacgtgcc ccagttcggg
    12721 caaggtcatc ctggagacgc ttaacttctc cgccgccgat ctgccgctgg actacgtggg
    12781 tctggccatt ctcatcgtga gcttccgggt gctcgcatat ctggctctaa gacttcgggc
    12841 ccgacgcaag gagtagccga catatatccg aaataactgc ttgttttttt ttttaccatt
    12901 attaccatcg tgtttactgt ttattgcccc ctcaaaaagc taatgtaatt atatttgtgc
    12961 caataaaaac aagatatgac ctatagaata caagtatttc cccttcgaac atccccacaa
    13021 gtagactttg gatttgtctt ctaaccaaaa gacttacaca cctgcatacc ttacatcaaa
    13081 aactcgttta tcgctacata aaacaccggg atatattttt tatatacata cttttcaaat
    13141 cgcgcgccct cttcataatt cacctccacc acaccacgtt tcgtagttgc tctttcgctg
    13201 tctcccaccc gctctccgca acacattcac cttttgttcg acgaccttgg agcgactgtc
    13261 gttagttccg cgcgattcgg ttcgctcaaa tggttccgag tggttcattt cgtctcaata
    13321 gaaattagta ataaatattt gtatgtacaa tttatttgct ccaatatatt tgtatatatt
    13381 tccctcacag ctatatttat tctaatttaa tattatgact ttttaaggta attttttgtg
    13441 acctgttcgg agtgattagc gttacaattt gaactgaaag tgacatccag tgtttgttcc
    13501 ttgtgtagat gcatctcaaa aaaatggtgg gcataatagt gttgtttata tatatcaaaa
    13561 ataacaacta taataataag aatacattta atttagaaaa tgcttggatt tcactggaac
    13621 tagaattaat tcggctgctg ctctaaacga cgcatttcgt actccaaagt acgaattttt
    13681 tccctcaagc tcttattttc attaaacaat gaacaggacc taacgcacag tcacgttatt
    13741 gtttacataa atgatttttt ttactattca aacttactct gtttgtgtac tcccactggt
    13801 atagccttct tttatctttt ctggttcagg ctctatcact ttactaggta cggcatctgc
    13861 gttgagtcgc ctccttttaa atgtctgacc ttttgcaggt gcagccttcc actgcgaatc
    13921 attaaagtgg gtatcacaaa tttgggagtt ttcaccaagg ctgcacccaa ggctctgctc
    13981 ccacaatttt ctcttaatag cacacttcgg cacgtgaatt aattttactc cagtcacagc
    14041 tttgcagcaa aatttgcaat atttcatttt tttttattcc acgtaagggt taatgttttc
    14101 aaaaaaaaat tcgtccgcac acaacctttc ctctcaacaa gcaaacgtgc actgaattta
    14161 agtgtatact tcggtaagct tcggctatcg acgggaccac cttatgttat ttcatcatgg
    14221 gccagaccca cgtagtccag cggcagatcg gcggcggaga agttaagcgt ctccaggatg
    14281 accttgcccg aactggggca cgtggtgttc gacgatgtgc agctaatttc gcccggctcc
    14341 acgtccgccc attggttaat cagcagaccc tcgttggcgt aacggaacca tgagaggtac
    14401 gacaaccatt tgaggtatac tggcaccgag cccgagttca agaagaaggc gtttttccat
    14461 aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac
    14521 ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct
    14581 gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg
    14641 ctttctcaat gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg
    14701 ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt
    14761 cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg
    14821 attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac
    14881 ggctacacta gaaggacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga
    14941 aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt
    15001 gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt
    15061 tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga
    15121 ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc
    15181 taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct
    15241 atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata
    15301 actacgatac gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca
    15361 cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga
    15421 agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga
    15481 gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg
    15541 gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga
    15601 gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt
    15661 gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct
    15721 cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca
    15781 ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaac acgggataat
    15841 accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga
    15901 aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc
    15961 aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg
    16021 caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc
    16081 ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt
    16141 gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca
    16201 cctgacgtct aagaaaccat tattatcatg acattaacct ataaaaatag gcgtatcacg
    16261 aggccctttc gtctcgcgcg tttcggtgat gacggtgaaa acctctgaca catgcagctc
    16321 ccggagacgg tcacagcttg tctgtaagcg gatgccggga gcagacaagc ccgtcagggc
    16381 gcgtcagcgg gtgttggcgg gtgtcggggc tggcttaact atgcggcatc agagcagatt
    16441 gtactgagag tgcaccatat cgacatgccc gccgtgaccg tcgagaaccc gctgacgctg
    16501 ccccgcgtat ccgcacccgc cgacgccgtc gcacgtcccg tgctcaccgt gaccaccgcg
    16561 cccagcggtt tcgagggcga gggcttcccg gtgcgccgcg cgttcgccgg gatcaactac
    16621 cgccacctcg acccgttcat catgatggac cagatgggtg aggtggagta cgcgcccggg
    16681 gagcccaagg gcacgccctg gcacccgcac cgcggcttcg agaccgtgac ctacatcgtc
    16741 gatatgcggt gtgaaatacc gcaccgaatc gcgcggaact aacgacagtc gctccaaggt
    16801 cgtcgaacaa aaggtgaatg tgttgcggag agcgggtggg agacagcgaa agagcaacta
    16861 cgaaacgtgg tgtggtggag gtgaattatg aagagggcgc gcgatttgaa aagtatgtat
    16921 ataaaaaata tatcccggtg ttttatgtag cgataaacga gtttttgatg taaggtatgc
    16981 aggtgtgtaa gtcttttggt tagaagacaa atccaaagtc tacttgtggg gatgttcgaa
    17041 ggggaaatac ttgtattcta taggtcatat cttgttttta ttggcacaaa tataattaca
    17101 ttagcttttt gagggggcaa taaacagtaa acacgatggt aataatggta aaaaaaaaaa
    17161 caagcagtta tttcggatat atgtcggcta ctccttgcgt cgggcccgaa gtcttagagc
    17221 cagatatgcg agcacccgga agctcacgat gagaatggcc agac
//
