LOCUS       pBL069_-_[ColE1]_PTet_s 3097 bp ds-DNA     circular     16-SEP-2022
DEFINITION  .
ACCESSION   urn.local...19-9evjh0t
SOURCE      null
COMMENT     Imported using the Genbank importer. File name: pTW161a.gb [ColE1]
            araC pBAD SD8 CadC DBD Leu16 GCN4 ZIP aadA. Source name: pAB121a -
            CP colEI Rop araC pBAD SD8rpoZ-cMyc-CRTC2 aadA (Note: Geneious
            sometimes stores the sequence name in the source attribute) Source
            name: pAB055k - AP pSC101 rrnB1(full) pTet SD8luxAB bla (Note:
            Geneious sometimes stores the sequence name in the source
            attribute) Source name: pAB055k - AP pSC101 rrnB1(full) pTet
            SD8luxAB bla (Note: Geneious sometimes stores the sequence name in
            the source attribute) Source name: pTW002a - CP ColEI Rop dnak-P1
            SD8 LuxABtetA-orfL aadA (Note: Geneious sometimes stores the
            sequence name in the source attribute) Source name: pTR4 - AP SC101
            PhaPO g3-luxAB TetR SD8PhaR-2 Term (Note: Geneious sometimes stores
            the sequence name in the source attribute)
FEATURES             Location/Qualifiers
     origin               1..588
                          /label="ColE1 origin of replication"
                          /ApEinfo_revcolor="#ffef86"
                          /ApEinfo_fwdcolor="#ffef86"
     CDS                  complement(1016..1207)
                          /label="Rop"
                          /ApEinfo_revcolor="#6ecff6"
                          /ApEinfo_fwdcolor="#6ecff6"
     terminator           1318..1362
                          /label="rrnB1"
                          /ApEinfo_revcolor="#f58a5e"
                          /ApEinfo_fwdcolor="#f58a5e"
     protein binding site 1404..1422
                          /label="tetR"
                          /ApEinfo_revcolor="#f6989d"
                          /ApEinfo_fwdcolor="#f6989d"
     promoter             1423..1458
                          /label="Ptet"
                          /ApEinfo_revcolor="#c4df9b"
                          /ApEinfo_fwdcolor="#c4df9b"
     protein binding site 1458..1476
                          /label="tetR"
                          /ApEinfo_revcolor="#f6989d"
                          /ApEinfo_fwdcolor="#f6989d"
     RBS                  1524..1537
                          /label="sd8"
                          /ApEinfo_revcolor="#b7e6d7"
                          /ApEinfo_fwdcolor="#b7e6d7"
     gene                 1538..2002
                          /label="cadC 1-155"
                          /ApEinfo_revcolor="#f8d3a9"
                          /ApEinfo_fwdcolor="#f8d3a9"
     CDS                  1538..2125
                          /label="Translation 1538-2125"
                          /translation="MQQPVVRVGEWLVTPSINQISRNGRQLTLRPRLIDLLVFFAQHSGEVLSRDELIDNVWKRSIVTNHVVTQSISELRKSLKDNDEDSPVYIATVPKRGYKLMVPVIWYSEEEGEEIMLSSAAAISEAVPATDSPSHSLNIQNTATPPEQSPVKSKRGGSGGLQRMKQLEDKVEELLSKNYHLENEVARLKKLVGER*"
     misc_feature         1625..1627
                          /label="E30R"
                          /ApEinfo_revcolor="#f58a5e"
                          /ApEinfo_fwdcolor="#f58a5e"
     misc_feature         1895..1909
                          /label="AAAIS mutation"
                          /ApEinfo_revcolor="#f58a5e"
                          /ApEinfo_fwdcolor="#f58a5e"
     misc_feature         2003..2017
                          /label="GGSGG Linker"
                          /ApEinfo_revcolor="#75c6a9"
                          /ApEinfo_fwdcolor="#75c6a9"
     gene                 2018..2125
                          /label="GCN4 ZIP wt"
                          /ApEinfo_revcolor="#75c6a9"
                          /ApEinfo_fwdcolor="#75c6a9"
     terminator           2150..2191
                          /label="tetA/orfL bidirectional"
                          /ApEinfo_revcolor="#f58a5e"
                          /ApEinfo_fwdcolor="#f58a5e"
     CDS                  complement(2205..2996)
                          /label="aadA"
                          /ApEinfo_revcolor="#6ecff6"
                          /ApEinfo_fwdcolor="#6ecff6"
     promoter             complement(2997..3081)
                          /label="aadA promoter"
                          /ApEinfo_revcolor="#c4df9b"
                          /ApEinfo_fwdcolor="#c4df9b"
ORIGIN
        1 tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca
       61 gcggtggttt gtttgccgga tcaagagcta ccacctcttt ttccgaaggt aactggcttc
      121 agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc
      181 aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct
      241 gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag
      301 gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc
      361 tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg
      421 agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag
      481 cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt
      541 gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac
      601 gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg
      661 ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc
      721 cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg
      781 cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatggtg cactctcagt
      841 acaatctgct ctgatgccgc atagttaagc cagtatacac tccgctatcg ctacgtgact
      901 gggtcatggc tgcgccccga cacccgccaa cacccgctga cgcgccctga cgggcttgtc
      961 tgctcccggc atccgcttac agacaagctg tgaccgtctc cgggagctgc atgtgtcaga
     1021 ggttttcacc gtcatcaccg aaacgcgcga ggcagctgcg gtaaagctca tcagcgtggt
     1081 cgtgaagcga ttcacagatg tctgcctgtt catccgcgtc cagctcgttg agtttctcca
     1141 gaagcgttaa tgtctggctt ctgataaagc gggccatgtt aagggcggtt ttttcctgtt
     1201 tggtcactga tgcctccgtg taagggggat ttctgttcat gggggtaatg ataccgatga
     1261 aacgagagag gatgctcacg atacgggtta ctgatgatga acaagaggac atccggtcaa
     1321 ataaaacgaa aggctcagtc gaaagactgg gcctttcgtt ttgctgagga gacttaggga
     1381 ccctccatcg aatggccaga tgatccctat cagtgataga gattgacact ctatcattga
     1441 tagagttatt ttaccactcc ctatcagtga tagagaaaag tgaaatgaat agttcgacaa
     1501 aaatctagac ctgcaggtgc agtaaaggaa aaaaaaaatg caacaacctg tagttcgcgt
     1561 tggcgaatgg cttgttactc cgtccataaa ccaaattagc cgcaatgggc gtcaacttac
     1621 ccttCGTccg agattaatcg atcttctggt tttctttgct caacacagtg gcgaagtact
     1681 tagcagggat gaacttatcg ataatgtctg gaagagaagt attgtcacca atcacgttgt
     1741 gacgcagagt atctcagaac tacgtaagtc attaaaagat aatgatgaag atagtcctgt
     1801 ctatatcgct actgtaccaa agcgcggcta taaattaatg gtgccggtta tctggtacag
     1861 cgaagaagag ggagaggaaa taatgctatc ttcgGCAGCG GCTATCTCTg aggcggttcc
     1921 tgccacagat tctccctccc acagtcttaa cattcaaaac accgcaacgc cacctgaaca
     1981 atccccagtt aaaagcaaac gaggtggATC TGGTGGTttg caaagaatga aacaacttga
     2041 agacaaggtt gaagaattgc tttcgaaaaa ttatcacttg gaaaatgagg ttgccagatt
     2101 aaagaaatta gttggcgaac gctaaactta attaacggca ctcctcagca aatataatga
     2161 ccctcttgat aacccaagag ggcatttttt aatgcccatg gcgtttattt gccgactacc
     2221 ttggtgatct cgcctttcac gtagtggaca aattcttcca actgatctgc gcgcgaggcc
     2281 aagcgatctt cttcttgtcc aagataagcc tgtctagctt caagtatgac gggctgatac
     2341 tgggccggca ggcgctccat tgcccagtcg gcagcgacat ccttaggcgc gattttgccg
     2401 gttactgcgc tgtaccaaat gcgggacaac gtaagcacta catttcgctc atcgccagcc
     2461 cagtcgggcg gcgagttcca tagcgttaag gtttcattta gcgcctcaaa tagatcctgt
     2521 tcaggaaccg gatcaaagag ttcctccgcc gctggaccta ccaaggcaac gctatgttct
     2581 cttgcttttg tcagcaagat agccagatca atgtcgatcg tggctggctc gaagatacct
     2641 gcaagaatgt cattgcgctg ccattctcca aattgcagtt cgcgcttagc tggataacgc
     2701 caaggaatga tgtcgtcgtg cacaacaatg gtgacttcta cagcgcggag aatctcgctc
     2761 tctccagggg aagccgaagt ttccaaaagg tcgttgatca aagctcgccg cgttgtttca
     2821 tcaagcctta cggtcaccgt aaccagcaaa tcaatatcac tgtgtggctt caggccgcca
     2881 tccactgcgg agccgtacaa atgtacggcc agcaacgtcg gttcgagatg gcgctcgatg
     2941 acgccaacta cctctgatag ttgagtcgat acttcggcga tcaccgcttc cctcatactc
     3001 ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag cggatacata
     3061 tttgaatgta tttagaaaaa taggccaaat aggccgt
//