LOCUS       pTW160c_-_[SC101]_Pcad3 5421 bp ds-DNA     circular     07-SEP-2022
DEFINITION  .
ACCESSION   urn.local...1w5-aqel8d9
SOURCE      null
COMMENT     Imported using the Genbank importer. File name: pTW160c.gb [SC101]
            pCadABc luxAB bla. Source name: pTW002a - CP ColEI Rop dnak-P1 SD8
            LuxABtetA-orfL aadA (Note: Geneious sometimes stores the sequence
            name in the source attribute) Source name: pTW001b - AP SC101 RepA
            Ptet SD8 MBP(wt) bla (Note: Geneious sometimes stores the sequence
            name in the source attribute) Source name: pTW002a - CP ColEI Rop
            dnak-P1 SD8 LuxABtetA-orfL aadA (Note: Geneious sometimes stores
            the sequence name in the source attribute) Source name: pTR4 - AP
            SC101 PhaPO g3-luxAB TetR SD8PhaR-2 Term (Note: Geneious sometimes
            stores the sequence name in the source attribute) Source name:
            pTW002a - CP ColEI Rop dnak-P1 SD8 LuxABtetA-orfL aadA (Note:
            Geneious sometimes stores the sequence name in the source
            attribute) Source name: pTW002a - CP ColEI Rop dnak-P1 SD8
            LuxABtetA-orfL aadA (Note: Geneious sometimes stores the sequence
            name in the source attribute) Source name: pTW001b - AP SC101 RepA
            Ptet SD8 MBP(wt) bla (Note: Geneious sometimes stores the sequence
            name in the source attribute)
FEATURES             Location/Qualifiers
     CDS             complement(1..951)
                     /label="repA"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     origin          complement(955..1700)
                     /label="SC101"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     USER junction   1704..1715
                     /label="USER006"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     terminator      1716..1760
                     /label="rrnB1 short"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     USER junction   1768..1782
                     /label="USER008"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     protein_bind    1805..1819
                     /label="H-NS"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     protein_bind    1886..1900
                     /label="ArcA"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     protein_bind    1890..1904
                     /label="H-NS"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     misc_feature    1917..2072
                     /label="Promoter"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     protein_bind    1936..1950
                     /label="H-NS"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
     -35_signal      2033..2038
                     /label=-35
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     protein_bind    2033..2047
                     /label="H-NS"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     gene            2034..2064
                     /label="Pcad gene"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     regulatory      2034..2064
                     /label="Pcad regulatory"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
     -10_signal      2059..2064
                     /label=-10
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     regulatory      2072..2072
                     /label="+1 sigma70"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     RBS             2136..2146
                     /label="cadB RBS"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     CDS             2147..4270
                     /label="luxAB"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     sig_peptide     3230..3289
                     /label="GGGGS4 flexible linker"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     USER junction   4276..4287
                     /label="USER002"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     terminator      complement(4295..4336)
                     /label="tetA/orfL bidirectional"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     USER junction   4337..4349
                     /label="USER005"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     CDS             complement(4350..5210)
                     /label="bla"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     promoter        complement(5211..5405)
                     /label="bla promoter"
                     /ApEinfo_revcolor="#c4df9b"
                     /ApEinfo_fwdcolor="#c4df9b"
     USER junction   5406..5421
                     /label="USER010"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
ORIGIN
        1 tcagatcctt ccgtatttag ccagtatgtt ctctagtgtg gttcgttgtt tttgcgtgag
       61 ccatgagaac gaaccattga gatcatgctt actttgcatg tcactcaaaa attttgcctc
      121 aaaactggtg agctgaattt ttgcagttaa agcatcgtgt agtgtttttc ttagtccgtt
      181 acgtaggtag gaatctgatg taatggttgt tggtattttg tcaccattca tttttatctg
      241 gttgttctca agttcggtta cgagatccat ttgtctatct agttcaactt ggaaaatcaa
      301 cgtatcagtc gggcggcctc gcttatcaac caccaatttc atattgctgt aagtgtttaa
      361 atctttactt attggtttca aaacccattg gttaagcctt ttaaactcat ggtagttatt
      421 ttcaagcatt aacatgaact taaattcatc aaggctaatc tctatatttg ccttgtgagt
      481 tttcttttgt gttagttctt ttaataacca ctcataaatc ctcatagagt atttgttttc
      541 aaaagactta acatgttcca gattatattt tatgaatttt tttaactgga aaagataagg
      601 caatatctct tcactaaaaa ctaattctaa tttttcgctt gagaacttgg catagtttgt
      661 ccactggaaa atctcaaagc ctttaaccaa aggattcctg atttccacag ttctcgtcat
      721 cagctctctg gttgctttag ctaatacacc ataagcattt tccctactga tgttcatcat
      781 ctgagcgtat tggttataag tgaacgatac cgtccgttct ttccttgtag ggttttcaat
      841 cgtggggttg agtagtgcca cacagcataa aattagcttg gtttcatgct ccgttaagtc
      901 atagcgacta atcgctagtt catttgcttt gaaaacaact aattcagaca tacatctcaa
      961 ttggtctagg tgattttaat cactatacca attgagatgg gctagtcaat gataattact
     1021 agtccttttc ctttgagttg tgggtatctg taaattctgc tagacctttg ctggaaaact
     1081 tgtaaattct gctagaccct ctgtaaattc cgctagacct ttgtgtgttt tttttgttta
     1141 tattcaagtg gttataattt atagaataaa gaaagaataa aaaaagataa aaagaataga
     1201 tcccagccct gtgtataact cactacttta gtcagttccg cagtattaca aaaggatgtc
     1261 gcaaacgctg tttgctcctc tacaaaacag accttaaaac cctaaaggct taagtagcac
     1321 cctcgcaagc tcgggcaaat cgctgaatat tccttttgtc tccgaccatc aggcacctga
     1381 gtcgctgtct ttttcgtgac attcagttcg ctgcgctcac ggctctggca gtgaatgggg
     1441 gtaaatggca ctacaggcgc cttttatgga ttcatgcaag gaaactaccc ataatacaag
     1501 aaaagcccgt cacgggcttc tcagggcgtt ttatggcggg tctgctatgt ggtgctatct
     1561 gactttttgc tgttcagcag ttcctgccct ctgattttcc agtctgacca cttcggatta
     1621 tcccgtgaca ggtcattcag actggctaat gcacccagta aggcagcggt atcatcaaca
     1681 ggcttacccg tcttactgtc aagaggacat ccggtcaaat aaaacgaaag gctcagtcga
     1741 aagactgggc ctttcgtttt gctgaggaga cttagggacc ctgtaactcc gggttgattt
     1801 atgctcggaa atatttgttg ttgagttttt gtatgttcct gttggtataa tatgttgcgg
     1861 caatttattt gccgcataat ttttattaca taaatttaac cagagaatgt cacgcaatcc
     1921 attgtaaaca ttaaatgttt atcttttcat gatatcaact tgcgatcctg atgtgttaat
     1981 aaaaaacctc aagttctcac ttacagaaac ttttgtgtta tttcacctaa tctttaggat
     2041 taatcctttt ttcgtgagta atcttatcgc cagtttggtc tggtcaggaa atagttatac
     2101 atcatgaccc ggactccaaa ttcaaaaatg aaattaggag aagagcatga aatttggaaa
     2161 ctttttgctt acataccaac ctccccaatt ttcccaaaca gaggtaatga aacgtttggt
     2221 taaattaggt cgcatctctg aggagtgtgg ttttgatacc gtatggttac tggagcatca
     2281 tttcacggag tttggtttgc ttggtaaccc ttatgtcgct gctgcatatt tacttggcgc
     2341 gactaaaaaa ttgaatgtag gaactgccgc tattgttctt cccacagccc atccagtacg
     2401 ccaacttgaa gatgtgaatt tattggatca aatgtcaaaa ggacgatttc ggtttggtat
     2461 ttgccgaggg ctttacaaca aggactttcg cgtattcggc acagatatga ataacagtcg
     2521 cgccttagcg gaatgctggt acgggctgat aaagaatggc atgacagagg gatatatgga
     2581 agctgataat gaacatatca agttccataa ggtaaaagta aaccccgcgg cgtatagcag
     2641 aggtggcgca ccggtttatg tggtggctga atcagcttcg acgactgagt gggctgctca
     2701 atttggccta ccgatgatat taagttggat tataaatact aacgaaaaga aagcacaact
     2761 tgagctttat aatgaagtgg ctcaagaata tgggcacgat attcataata tcgaccattg
     2821 cttatcatat ataacatctg tagatcatga ctcaattaaa gcgaaagaga tttgccggaa
     2881 atttctgggg cattggtatg attcttatgt gaatgctacg actatttttg atgattcaga
     2941 ccaaacaaga ggttatgatt tcaataaagg gcagtggcgt gactttgtat taaaaggaca
     3001 taaagatact aatcgccgta ttgattacag ttacgaaatc aatcccgtgg gaacgccgca
     3061 ggaatgtatt gacataattc aaaaagacat tgatgctaca ggaatatcaa atatttgttg
     3121 tggatttgaa gctaatggaa cagtagacga aattattgct tccatgaagc tcttccagtc
     3181 tgatgtcatg ccatttctta aagaaaaaca acgttcgcta ttatattatg gcggtggcgg
     3241 tagcggcggt ggcggtagcg gcggtggcgg tagcggcggt ggcggtagca aatttggatt
     3301 gttcttcctt aacttcatca attcaacaac tgttcaagaa cagagtatag ttcgcatgca
     3361 ggaaataacg gagtatgttg ataagttgaa ttttgaacag attttagtgt atgaaaatca
     3421 tttttcagat aatggtgttg tcggcgctcc tctgactgtt tctggttttc tgctcggttt
     3481 aacagagaaa attaaaattg gttcattaaa tcacatcatt acaactcatc atcctgtccg
     3541 catagcggag gaagcttgct tattggatca gttaagtgaa gggagattta ttttagggtt
     3601 tagtgattgc gaaaaaaaag atgaaatgca tttttttaat cgcccggttg aatatcaaca
     3661 gcaactattt gaagagtgtt atgaaatcat taacgatgct ttaacaacag gctattgtaa
     3721 tccagataac gatttttata gcttccctaa aatatctgta aatccccatg cttatacgcc
     3781 aggcggacct cggaaatatg taacagcaac cagtcatcat attgttgagt gggcggccaa
     3841 aaaaggtatt cctctcatct ttaagtggga tgattctaat gatgttagat atgaatatgc
     3901 tgaaagatat aaagccgttg cggataaata tgacgttgac ctatcagaga tagaccatca
     3961 gttaatgata ttagttaact ataacgaaga tagtaataaa gctaaacaag agacgcgtgc
     4021 atttattagt gattatgttc ttgaaatgca ccctaatgaa aatttcgaaa ataaacttga
     4081 agaaataatt gcagaaaacg ctgtcggaaa ttatacggag tgtataactg cggctaagtt
     4141 ggcaattgaa aagtgtggtg cgaaaagtgt attgctgtcc tttgaaccaa tgaatgattt
     4201 gatgagccaa aaaaatgtaa tcaatattgt tgatgataat attaagaagt accacacgga
     4261 atatacctaa acttaattaa cggcactcct cagcaaatat aatgaccctc ttgataaccc
     4321 aagagggcat tttttaatgc ccatggcgtt taccaatgct taatcagtga ggcacctatc
     4381 tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact
     4441 acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc
     4501 tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt
     4561 ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta
     4621 agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg
     4681 tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt
     4741 acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc
     4801 agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt
     4861 actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc
     4921 tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc
     4981 gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa
     5041 ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac
     5101 tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa
     5161 aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt
     5221 tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa
     5281 tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct
     5341 gacgtctaag aaaccattat tatcatgaca ttaacctata aaaataggcg tatcacgagg
     5401 cccttaggcc aaataggccg t
//