LOCUS       Exported               15631 bp ds-DNA     circular SYN 16-DEC-2019
DEFINITION  cloning vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 15631)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Thursday, Mar 19, 2020 from SnapGene 5.0.4
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..15631
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     promoter        212..816
                     /locus_tag="CMV promoter/enhancer"
                     /label=CMV promoter/enhancer
     LTR             835..1509
                     /locus_tag="5' HIVR-U5"
                     /label=5' HIVR-U5
     misc_binding    1533..2390
                     /locus_tag="HIV rev response element"
                     /label=HIV rev response element
     misc_binding    2416..2593
                     /locus_tag="HIV flap/nuclear import signal"
                     /label=HIV flap/nuclear import signal
     primer_bind     2558..2577
                     /locus_tag="BX-Seq073-gblock+TRE3G promoter"
                     /label=BX-Seq073-gblock+TRE3G promoter
     misc_feature    2600..2606
                     /locus_tag="U6 terminator"
                     /label=U6 terminator
     misc_feature    2607..2629
                     /locus_tag="crRNA for targeted CTCF_1"
                     /label=crRNA for targeted CTCF_1
     misc_feature    2630..2649
                     /locus_tag="DR for LbCpf1"
                     /label=DR for LbCpf1
     misc_feature    2650..2672
                     /locus_tag="crRNA for targeted CTCF_2"
                     /label=crRNA for targeted CTCF_2
     misc_feature    2673..2692
                     /locus_tag="DR for LbCpf1(1)"
                     /label=DR for LbCpf1
                     /label=DR for LbCpf1(1)
     promoter        complement(2702..2958)
                     /locus_tag="human U6 promoter"
                     /label=human U6 promoter
     primer_bind     complement(2931..2951)
                     /locus_tag="CX_Seq018-yarra"
                     /label=CX_Seq018-yarra
     misc_feature    2959..3048
                     /locus_tag="linkage before CAG-cpf1 from 78743"
                     /label=linkage before CAG-cpf1 from 78743
     misc_feature    3049..3132
                     /locus_tag="linkage from pX330 between gRNA and CMV
                     enhancer"
                     /label=linkage from pX330 between gRNA and CMV
                     enhancer
                     /label=linkage from pX330 between gRNA and CMV enhancer
     promoter        3159..3534
                     /locus_tag="TRE3G promoter"
                     /label=TRE3G promoter
     misc_feature    3164..3183
                     /locus_tag="TRE operator sequence"
                     /label=TRE operator sequence
     misc_feature    3200..3219
                     /locus_tag="TRE operator sequence-2"
                     /label=TRE operator sequence-2
     primer_bind     3220..3239
                     /locus_tag="BX-Seq074-gblock+TRE3G promoter"
                     /label=BX-Seq074-gblock+TRE3G promoter
     misc_feature    3236..3255
                     /locus_tag="TRE operator sequence-3"
                     /label=TRE operator sequence-3
     misc_feature    3272..3291
                     /locus_tag="TRE operator sequence-4"
                     /label=TRE operator sequence-4
     misc_feature    3308..3327
                     /locus_tag="TRE operator sequence-5"
                     /label=TRE operator sequence-5
     misc_feature    3344..3363
                     /locus_tag="TRE operator sequence-6"
                     /label=TRE operator sequence-6
     misc_feature    3380..3399
                     /locus_tag="TRE operator sequence-7"
                     /label=TRE operator sequence-7
     misc_feature    3559..3566
                     /locus_tag="Kozak"
                     /label=Kozak
     CDS             3564..7247
                     /codon_start=1
                     /locus_tag="LbCpf1"
                     /product="CRISPR-associated protein from Lachnospiraceae
                     bacterium ND2006 (Zetsche et al., 2015)"
                     /label=LbCpf1
                     /translation="MSKLEKFTNCYSLSKTLRFKAIPVGKTQENIDNKRLLVEDEKRAE
                     DYKGVKKLLDRYYLSFINDVLHSIKLKNLNNYISLFRKKTRTEKENKELENLEINLRKE
                     IAKAFKGNEGYKSLFKKDIIETILPEFLDDKDEIALVNSFNGFTTAFTGFFDNRENMFS
                     EEAKSTSIAFRCINENLTRYISNMDIFEKVDAIFDKHEVQEIKEKILNSDYDVEDFFEG
                     EFFNFVLTQEGIDVYNAIIGGFVTESGEKIKGLNEYINLYNQKTKQKLPKFKPLYKQVL
                     SDRESLSFYGEGYTSDEEVLEVFRNTLNKNSEIFSSIKKLEKLFKNFDEYSSAGIFVKN
                     GPAISTISKDIFGEWNVIRDKWNAEYDDIHLKKKAVVTEKYEDDRRKSFKKIGSFSLEQ
                     LQEYADADLSVVEKLKEIIIQKVDEIYKVYGSSEKLFDADFVLEKSLKKNDAVVAIMKD
                     LLDSVKSFENYIKAFFGEGKETNRDESFYGDFVLAYDILLKVDHIYDAIRNYVTQKPYS
                     KDKFKLYFQNPQFMGGWDKDKETDYRATILRYGSKYYLAIMDKKYAKCLQKIDKDDVNG
                     NYEKINYKLLPGPNKMLPKVFFSKKWMAYYNPSEDIQKIYKNGTFKKGDMFNLNDCHKL
                     IDFFKDSISRYPKWSNAYDFNFSETEKYKDIAGFYREVEEQGYKVSFESASKKEVDKLV
                     EEGKLYMFQIYNKDFSDKSHGTPNLHTMYFKLLFDENNHGQIRLSGGAELFMRRASLKK
                     EELVVHPANSPIANKNPDNPKKTTTLSYDVYKDKRFSEDQYELHIPIAINKCPKNIFKI
                     NTEVRVLLKHDDNPYVIGIARGERNLLYIVVVDGKGNIVEQYSLNEIINNFNGIRIKTD
                     YHSLLDKKEKERFEARQNWTSIENIKELKAGYISQVVHKICELVEKYDAVIALEDLNSG
                     FKNSRVKVEKQVYQKFEKMLIDKLNYMVDKKSNPCATGGALKGYQITNKFESFKSMSTQ
                     NGFIFYIPAWLTSKIDPSTGFVNLLKTKYTSIADSKKFISSFDRIMYVPEEDLFEFALD
                     YKNFSRTDADYIKKWKLYSYGNRIRIFRNPKKNNVFDWEEVCLTSAYKELFNKYGINYQ
                     QGDIRALLCEQSDKAFYSSFMALMSLMLQMRNSITGRTDVDFLISPVKNSDGIFYDSRN
                     YEAQENAILPKNADANGAYNIARKVLWAIGQFKKAEDEKLDKVKIAISNKEWLEYAQTS
                     VKH"
     primer_bind     3951..3971
                     /locus_tag="BX-Seq001-LbCpf1"
                     /label=BX-Seq001-LbCpf1
     primer_bind     4546..4565
                     /locus_tag="BX-Seq002-LbCpf1"
                     /label=BX-Seq002-LbCpf1
     primer_bind     5067..5087
                     /locus_tag="BX-Seq003-LbCpf1"
                     /label=BX-Seq003-LbCpf1
     primer_bind     5620..5640
                     /locus_tag="BX-Seq004-LbCpf1"
                     /label=BX-Seq004-LbCpf1
     primer_bind     6274..6293
                     /locus_tag="BX-Seq005-LbCpf1"
                     /label=BX-Seq005-LbCpf1
     primer_bind     6879..6900
                     /locus_tag="BX-Seq006-LbCpf1"
                     /label=BX-Seq006-LbCpf1
     misc_feature    7248..7253
                     /locus_tag="EcoRI"
                     /label=EcoRI
     misc_feature    7254..7268
                     /locus_tag="linker"
                     /label=linker
     misc_feature    7269..7289
                     /locus_tag="NLS"
                     /label=NLS
     misc_feature    7290..7292
                     /locus_tag="linker(1)"
                     /label=linker
                     /label=linker(1)
     misc_feature    7293..7313
                     /locus_tag="NLS(1)"
                     /label=NLS
                     /label=NLS(1)
     misc_feature    7314..7331
                     /locus_tag="linker(2)"
                     /label=linker
                     /label=linker(2)
     misc_feature    7316..8673
                     /label=gBlock
     misc_feature    7326..7331
                     /locus_tag="BamHI"
                     /label=BamHI
     CDS             7332..9509
                     /locus_tag="hCTCF_isoform1"
                     /label=hCTCF_isoform1
     misc_feature    7336..7353
                     /locus_tag="SL-912"
                     /label=SL-912
     misc_feature    7841..7858
                     /locus_tag="SL-913"
                     /label=SL-913
     misc_feature    8328..8345
                     /locus_tag="SL-914"
                     /label=SL-914
     misc_feature    8514..8516
                     /label=R396A
     misc_feature    8835..8852
                     /locus_tag="SL-915"
                     /label=SL-915
     misc_feature    9328..9345
                     /locus_tag="SL-916"
                     /label=SL-916
     misc_feature    9510..9515
                     /locus_tag="HpaI"
                     /label=HpaI
     misc_feature    9516..9545
                     /locus_tag="HA tag"
                     /label=HA tag
     misc_feature    9546..9602
                     /locus_tag="P2A"
                     /label=P2A
     misc_feature    9603..10304
                     /locus_tag="tagBFP"
                     /label=tagBFP
     misc_feature    10323..10911
                     /locus_tag="WRE"
                     /label=WRE
     misc_feature    10398..10412
                     /locus_tag="SL-54 sequencing primer rev"
                     /label=SL-54 sequencing primer rev
     LTR             10927..11622
                     /locus_tag="HIV delU3RdelU5"
                     /label=HIV delU3RdelU5
     misc_feature    12802..13175
                     /locus_tag="ZEO"
                     /label=ZEO
     misc_feature    14641..15499
                     /locus_tag="AMP"
                     /label=AMP
ORIGIN
        1 gtcgacggat cgggagatct cccgatcccc tatggtgcac tctcagtaca atctgctctg
       61 atgccgcata gttaagccag tatctgctcc ctgcttgtgt gttggaggtc gctgagtagt
      121 gcgcgagcaa aatttaagct acaacaaggc aaggcttgac cgacaattgc atgaagaatc
      181 tgcttagggt taggcgtttt gcgctgcttc gcgatgtacg ggccagatat acgcgttgac
      241 attgattatt gactagttat taatagtaat caattacggg gtcattagtt catagcccat
      301 atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg
      361 acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt
      421 tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag
      481 tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc
      541 attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag
      601 tcatcgctat taccatggtg atgcggtttt ggcagtacat caatgggcgt ggatagcggt
      661 ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc
      721 accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg
      781 gcggtaggcg tgtacggtgg gaggtctata taagcagcgc gttttgcctg tactgggtct
      841 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt
      901 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac
      961 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc
     1021 gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc
     1081 ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa
     1141 ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg
     1201 ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata
     1261 aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc
     1321 tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga
     1381 caggatcaga agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc
     1441 aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca
     1501 aaagtaagac caccgcacag caagcggccg ctgatcttca gacctggagg aggagatatg
     1561 agggacaatt ggagaagtga attatataaa tataaagtag taaaaattga accattagga
     1621 gtagcaccca ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc agtgggaata
     1681 ggagctttgt tccttgggtt cttgggagca gcaggaagca ctatgggcgc agcgtcaatg
     1741 acgctgacgg tacaggccag acaattattg tctggtatag tgcagcagca gaacaatttg
     1801 ctgagggcta ttgaggcgca acagcatctg ttgcaactca cagtctgggg catcaagcag
     1861 ctccaggcaa gaatcctggc tgtggaaaga tacctaaagg atcaacagct cctggggatt
     1921 tggggttgct ctggaaaact catttgcacc actgctgtgc cttggaatgc tagttggagt
     1981 aataaatctc tggaacagat ttggaatcac acgacctgga tggagtggga cagagaaatt
     2041 aacaattaca caagcttaat acactcctta attgaagaat cgcaaaacca gcaagaaaag
     2101 aatgaacaag aattattgga attagataaa tgggcaagtt tgtggaattg gtttaacata
     2161 acaaattggc tgtggtatat aaaattattc ataatgatag taggaggctt ggtaggttta
     2221 agaatagttt ttgctgtact ttctatagtg aatagagtta ggcagggata ttcaccatta
     2281 tcgtttcaga cccacctccc aaccccgagg ggacccgaca ggcccgaagg aatagaagaa
     2341 gaaggtggag agagagacag agacagatcc attcgattag tgaacggatc ggcactgcgt
     2401 gcgccaattc tgcagacaaa tggcagtatt catccacaat tttaaaagaa aaggggggat
     2461 tggggggtac agtgcagggg aaagaatagt agacataata gcaacagaca tacaaactaa
     2521 agaattacaa aaacaaatta caaaaattca aaattttcgg gtttattaca gggacagcag
     2581 agatccagtt tggttaatta aaaaaatgag cgggcctggg ccatgtccta tctacactta
     2641 gtagaaattg acccccttca catagagaga agatctacac ttagtagaaa ttgacgctag
     2701 ccgtcctttc cacaagatat ataaagccaa gaaatcgaaa tactttcaag ttacggtaag
     2761 catatgatag tccattttaa aacataattt taaaactgca aactacccaa gaaattatta
     2821 ctttctacgt cacgtatttt gtactaatat ctttgtgttt acagtcaaat taattctaat
     2881 tatctctcta acagccttgt atcgtatatg caaatatgaa ggaatcatgg gaaataggcc
     2941 ctcttcctgc ccgaccttat tgtctcatga gcggatacat atttgaatgt atttagaaaa
     3001 ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctggt tttagagcta
     3061 gaaatagcaa gttaaaataa ggctagtccg tttttagcgc gtgcgccaat tctgcagaca
     3121 aatggctcta gattcgaagt cttcaagaat tgctcgagtt tactccctat cagtgataga
     3181 gaacgtatga agagtttact ccctatcagt gatagagaac gtatgcagac tttactccct
     3241 atcagtgata gagaacgtat aaggagttta ctccctatca gtgatagaga acgtatgacc
     3301 agtttactcc ctatcagtga tagagaacgt atctacagtt tactccctat cagtgataga
     3361 gaacgtatat ccagtttact ccctatcagt gatagagaac gtataagctt taggcgtgta
     3421 cggtgggcgc ctataaaagc agagctcgtt tagtgaaccg tcagatcgcc tggagcaatt
     3481 ccacaacact tttgtcttat accaactttc cgtaccactt cctaccctcg taaaccgcgg
     3541 gcccgggttc caccggtgcc accatgagca agctggagaa gtttacaaac tgctactccc
     3601 tgtctaagac cctgaggttc aaggccatcc ctgtgggcaa gacccaggag aacatcgaca
     3661 ataagcggct gctggtggag gacgagaaga gagccgagga ttataagggc gtgaagaagc
     3721 tgctggatcg ctactatctg tcttttatca acgacgtgct gcacagcatc aagctgaaga
     3781 atctgaacaa ttacatcagc ctgttccgga agaaaaccag aaccgagaag gagaataagg
     3841 agctggagaa cctggagatc aatctgcgga aggagatcgc caaggccttc aagggcaacg
     3901 agggctacaa gtccctgttt aagaaggata tcatcgagac aatcctgcca gagttcctgg
     3961 acgataagga cgagatcgcc ctggtgaaca gcttcaatgg ctttaccaca gccttcaccg
     4021 gcttctttga taacagagag aatatgtttt ccgaggaggc caagagcaca tccatcgcct
     4081 tcaggtgtat caacgagaat ctgacccgct acatctctaa tatggacatc ttcgagaagg
     4141 tggacgccat ctttgataag cacgaggtgc aggagatcaa ggagaagatc ctgaacagcg
     4201 actatgatgt ggaggatttc tttgagggcg agttctttaa ctttgtgctg acacaggagg
     4261 gcatcgacgt gtataacgcc atcatcggcg gcttcgtgac cgagagcggc gagaagatca
     4321 agggcctgaa cgagtacatc aacctgtata atcagaaaac caagcagaag ctgcctaagt
     4381 ttaagccact gtataagcag gtgctgagcg atcgggagtc tctgagcttc tacggcgagg
     4441 gctatacatc cgatgaggag gtgctggagg tgtttagaaa caccctgaac aagaacagcg
     4501 agatcttcag ctccatcaag aagctggaga agctgttcaa gaattttgac gagtactcta
     4561 gcgccggcat ctttgtgaag aacggccccg ccatcagcac aatctccaag gatatcttcg
     4621 gcgagtggaa cgtgatccgg gacaagtgga atgccgagta tgacgatatc cacctgaaga
     4681 agaaggccgt ggtgaccgag aagtacgagg acgatcggag aaagtccttc aagaagatcg
     4741 gctccttttc tctggagcag ctgcaggagt acgccgacgc cgatctgtct gtggtggaga
     4801 agctgaagga gatcatcatc cagaaggtgg atgagatcta caaggtgtat ggctcctctg
     4861 agaagctgtt cgacgccgat tttgtgctgg agaagagcct gaagaagaac gacgccgtgg
     4921 tggccatcat gaaggacctg ctggattctg tgaagagctt cgagaattac atcaaggcct
     4981 tctttggcga gggcaaggag acaaacaggg acgagtcctt ctatggcgat tttgtgctgg
     5041 cctacgacat cctgctgaag gtggaccaca tctacgatgc catccgcaat tatgtgaccc
     5101 agaagcccta ctctaaggat aagttcaagc tgtattttca gaaccctcag ttcatgggcg
     5161 gctgggacaa ggataaggag acagactatc gggccaccat cctgagatac ggctccaagt
     5221 actatctggc catcatggat aagaagtacg ccaagtgcct gcagaagatc gacaaggacg
     5281 atgtgaacgg caattacgag aagatcaact ataagctgct gcccggccct aataagatgc
     5341 tgccaaaggt gttcttttct aagaagtgga tggcctacta taaccccagc gaggacatcc
     5401 agaagatcta caagaatggc acattcaaga agggcgatat gtttaacctg aatgactgtc
     5461 acaagctgat cgacttcttt aaggatagca tctcccggta tccaaagtgg tccaatgcct
     5521 acgatttcaa cttttctgag acagagaagt ataaggacat cgccggcttt tacagagagg
     5581 tggaggagca gggctataag gtgagcttcg agtctgccag caagaaggag gtggataagc
     5641 tggtggagga gggcaagctg tatatgttcc agatctataa caaggacttt tccgataagt
     5701 ctcacggcac acccaatctg cacaccatgt acttcaagct gctgtttgac gagaacaatc
     5761 acggacagat caggctgagc ggaggagcag agctgttcat gaggcgcgcc tccctgaaga
     5821 aggaggagct ggtggtgcac ccagccaact cccctatcgc caacaagaat ccagataatc
     5881 ccaagaaaac cacaaccctg tcctacgacg tgtataagga taagaggttt tctgaggacc
     5941 agtacgagct gcacatccca atcgccatca ataagtgccc caagaacatc ttcaagatca
     6001 atacagaggt gcgcgtgctg ctgaagcacg acgataaccc ctatgtgatc ggcatcgcga
     6061 ggggcgagcg caatctgctg tatatcgtgg tggtggacgg caagggcaac atcgtggagc
     6121 agtattccct gaacgagatc atcaacaact tcaacggcat caggatcaag acagattacc
     6181 actctctgct ggacaagaag gagaaggaga ggttcgaggc ccgccagaac tggacctcca
     6241 tcgagaatat caaggagctg aaggccggct atatctctca ggtggtgcac aagatctgcg
     6301 agctggtgga gaagtacgat gccgtgatcg ccctggagga cctgaactct ggctttaaga
     6361 atagccgcgt gaaggtggag aagcaggtgt atcagaagtt cgagaagatg ctgatcgata
     6421 agctgaacta catggtggac aagaagtcta atccttgtgc aacaggcggc gccctgaagg
     6481 gctatcagat caccaataag ttcgagagct ttaagtccat gtctacccag aacggcttca
     6541 tcttttacat ccctgcctgg ctgacatcca agatcgatcc atctaccggc tttgtgaacc
     6601 tgctgaaaac caagtatacc agcatcgccg attccaagaa gttcatcagc tcctttgaca
     6661 ggatcatgta cgtgcccgag gaggatctgt tcgagtttgc cctggactat aagaacttct
     6721 ctcgcacaga cgccgattac atcaagaagt ggaagctgta ctcctacggc aaccggatca
     6781 gaatcttccg gaatcctaag aagaacaacg tgttcgactg ggaggaggtg tgcctgacca
     6841 gcgcctataa ggagctgttc aacaagtacg gcatcaatta tcagcagggc gatatcagag
     6901 ccctgctgtg cgagcagtcc gacaaggcct tctactctag ctttatggcc ctgatgagcc
     6961 tgatgctgca gatgcggaac agcatcacag gccgcaccga cgtggatttt ctgatcagcc
     7021 ctgtgaagaa ctccgacggc atcttctacg atagccggaa ctatgaggcc caggagaatg
     7081 ccatcctgcc aaagaacgcc gacgccaatg gcgcctataa catcgccaga aaggtgctgt
     7141 gggccatcgg ccagttcaag aaggccgagg acgagaagct ggataaggtg aagatcgcca
     7201 tctctaacaa ggagtggctg gagtacgccc agaccagcgt gaagcacgaa ttcggtggcg
     7261 gaggctcgcc aaaaaagaag agaaaggtag gtccaaagaa aaaacgaaaa gtagatggtg
     7321 gcggaggatc cgaaggtgat gcagtcgaag ccattgtgga ggagtccgaa acttttatta
     7381 aaggaaagga gagaaagact taccagagac gccgggaagg gggccaggaa gaagatgcct
     7441 gccacttacc ccagaaccag acggatgggg gtgaggtggt ccaggatgtc aacagcagtg
     7501 tacagatggt gatgatggaa cagctggacc ccacccttct tcagatgaag actgaagtaa
     7561 tggagggcac agtggctcca gaagcagagg ctgctgtgga cgatacccag attataactt
     7621 tacaggttgt aaatatggag gaacagccca taaacatagg agaacttcag cttgttcaag
     7681 tacctgttcc tgtgactgta cctgttgcta ccacttcagt agaagaactt cagggggctt
     7741 atgaaaatga agtgtctaaa gagggccttg cggaaagtga acccatgata tgccacaccc
     7801 tacctttgcc tgaagggttt caggtggtta aagtgggggc caatggagag gtggagacac
     7861 tagaacaagg ggaacttcca ccccaggaag atcctagttg gcaaaaagac ccagactatc
     7921 agccaccagc caaaaaaaca aagaaaacca aaaagagcaa actgcgttat acagaggagg
     7981 gcaaagatgt agatgtgtct gtctacgatt ttgaggaaga acagcaggag ggtctgctat
     8041 cagaggttaa tgcagagaaa gtggttggta atatgaagcc tccaaagcca acaaaaatta
     8101 aaaagaaagg tgtaaagaag acattccagt gtgagctttg cagttacacg tgtccacggc
     8161 gttcaaattt ggatcgtcac atgaaaagcc acactgatga gagaccacac aagtgccatc
     8221 tctgtggcag ggcattcaga acagtcaccc tcctgaggaa tcaccttaac acacacacag
     8281 gtactcgtcc tcacaagtgc ccagactgcg acatggcctt tgtgaccagt ggagaattgg
     8341 ttcggcatcg tcgttacaaa cacacccacg agaagccatt caagtgttcc atgtgcgatt
     8401 acgccagtgt agaagtcagc aaattaaaac gtcacattcg ctctcatact ggagagcgtc
     8461 cgtttcagtg cagtttgtgc agttatgcca gcagggacac atacaagctg aaagcgcaca
     8521 tgagaaccca ttcaggggaa aagccttatg aatgttatat ttgtcatgct cggtttaccc
     8581 aaagtggtac catgaagatg cacattttac agaagcacac agaaaatgtg gccaaatttc
     8641 actgtcccca ctgtgacaca gtcatagccc gaaaaagtga tttgggtgtc cacttgcgaa
     8701 agcagcattc ctatattgag caaggcaaga aatgccgtta ctgtgatgct gtgtttcatg
     8761 agcgctatgc cctcatccag catcagaagt cacacaagaa tgagaagcgc tttaagtgtg
     8821 accagtgtga ttacgcttgt agacaggaga ggcacatgat catgcacaag cgcacccaca
     8881 ccggggagaa gccttacgcc tgcagccact gcgataagac cttccgccag aagcagcttc
     8941 tcgacatgca cttcaagcgc tatcacgacc ccaacttcgt ccctgcggct tttgtctgtt
     9001 ctaagtgtgg gaaaacattt acacgtcgga ataccatggc aagacatgct gataattgtg
     9061 ctggcccaga tggcgtagag ggggaaaatg gaggagaaac gaagaagagt aaacgtggaa
     9121 gaaaaagaaa gatgcgctct aagaaagaag attcctctga cagtgaaaat gctgaaccag
     9181 atctggacga caatgaggat gaggaggagc ctgccgtaga aattgaacct gagccagagc
     9241 ctcagcctgt gaccccagcc ccaccacccg ccaagaagcg gagaggacga ccccctggca
     9301 gaaccaacca gcccaaacag aaccagccaa cagctatcat tcaggttgaa gaccagaata
     9361 caggtgcaat tgagaacatt atagttgaag taaaaaaaga gccagatgct gagcccgcag
     9421 agggagagga agaggaggcc cagccagctg ccacagatgc ccccaacgga gacctcacgc
     9481 ccgagatgat cctcagcatg atggaccggg ttaacgcata cccctacgac gtccccgact
     9541 acgccgccac gaacttctct ctgttaaagc aagcaggaga tgttgaagaa aaccccgggc
     9601 ctatgagcga gctgattaag gagaacatgc acatgaagct gtacatggag ggcaccgtgg
     9661 acaaccatca cttcaagtgc acatccgagg gcgaaggcaa gccctacgag ggcacccaga
     9721 ccatgagaat caaggtggtc gagggcggcc ctctcccctt cgccttcgac atcctggcta
     9781 ctagcttcct ctacggcagc aagaccttca tcaaccacac ccagggcatc cccgacttct
     9841 tcaagcagtc cttccctgag ggcttcacat gggagagagt caccacatac gaagacgggg
     9901 gcgtgctgac cgctacccag gacaccagcc tccaggacgg ctgcctcatc tacaacgtca
     9961 agatcagagg ggtgaacttc acatccaacg gccctgtgat gcagaagaaa acactcggct
    10021 gggaggcctt caccgagacg ctgtaccccg ctgacggcgg cctggaaggc agaaacgaca
    10081 tggccctgaa gctcgtgggc gggagccatc tgatcgcaaa catcaagacc acatatagat
    10141 ccaagaaacc cgctaagaac ctcaagatgc ctggcgtcta ctatgtggac tacagactgg
    10201 aaagaatcaa ggaggccaac aacgagacct acgtcgagca gcacgaggtg gcagtggcca
    10261 gatactgcga cctccctagc aaactggggc acaagcttaa ttgagatatc aagcttatcg
    10321 ataatcaacc tctggattac aaaatttgtg aaagattgac tggtattctt aactatgttg
    10381 ctccttttac gctatgtgga tacgctgctt taatgccttt gtatcatgct attgcttccc
    10441 gtatggcttt cattttctcc tccttgtata aatcctggtt gctgtctctt tatgaggagt
    10501 tgtggcccgt tgtcaggcaa cgtggcgtgg tgtgcactgt gtttgctgac gcaaccccca
    10561 ctggttgggg cattgccacc acctgtcagc tcctttccgg gactttcgct ttccccctcc
    10621 ctattgccac ggcggaactc atcgccgcct gccttgcccg ctgctggaca ggggctcggc
    10681 tgttgggcac tgacaattcc gtggtgttgt cggggaaatc atcgtccttt ccttggctgc
    10741 tcgcctgtgt tgccacctgg attctgcgcg ggacgtcctt ctgctacgtc ccttcggccc
    10801 tcaatccagc ggaccttcct tcccgcggcc tgctgccggc tctgcggcct cttccgcgtc
    10861 ttcgccttcg ccctcagacg agtcggatct ccctttgggc cgcctccccg catcgatacc
    10921 gtcgacctcg agacctagaa aaacatggag caatcacaag tagcaataca gcagctacca
    10981 atgctgattg tgcctggcta gaagcacaag aggaggagga ggtgggtttt ccagtcacac
    11041 ctcaggtacc tttaagacca atgacttaca aggcagctgt agatcttagc cactttttaa
    11101 aagaaaaggg gggactggaa gggctaattc actcccaacg aagacaagat atccttgatc
    11161 tgtggatcta ccacacacaa ggctacttcc ctgattggca gaactacaca ccagggccag
    11221 ggatcagata tccactgacc tttggatggt gctacaagct agtaccagtt gagcaagaga
    11281 aggtagaaga agccaatgaa ggagagaaca cccgcttgtt acaccctgtg agcctgcatg
    11341 ggatggatga cccggagaga gaagtattag agtggaggtt tgacagccgc ctagcatttc
    11401 atcacatggc ccgagagctg catccggact gtactgggtc tctctggtta gaccagatct
    11461 gagcctggga gctctctggc taactaggga acccactgct taagcctcaa taaagcttgc
    11521 cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc
    11581 tcagaccctt ttagtcagtg tggaaaatct ctagcagggc ccgtttaaac ccgctgatca
    11641 gcctcgactg tgccttctag ttgccagcca tctgttgttt gcccctcccc cgtgccttcc
    11701 ttgaccctgg aaggtgccac tcccactgtc ctttcctaat aaaatgagga aattgcatcg
    11761 cattgtctga gtaggtgtca ttctattctg gggggtgggg tggggcagga cagcaagggg
    11821 gaggattggg aagacaatag caggcatgct ggggatgcgg tgggctctat ggcttctgag
    11881 gcggaaagaa ccagctgggg ctctaggggg tatccccacg cgccctgtag cggcgcatta
    11941 agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag cgccctagcg
    12001 cccgctcctt tcgctttctt cccttccttt ctcgccacgt tcgccggctt tccccgtcaa
    12061 gctctaaatc gggggctccc tttagggttc cgatttagtg ctttacggca cctcgacccc
    12121 aaaaaacttg attagggtga tggttcacgt agtgggccat cgccctgata gacggttttt
    12181 cgccctttga cgttggagtc cacgttcttt aatagtggac tcttgttcca aactggaaca
    12241 acactcaacc ctatctcggt ctattctttt gatttataag ggattttgcc gatttcggcc
    12301 tattggttaa aaaatgagct gatttaacaa aaatttaacg cgaattaatt ctgtggaatg
    12361 tgtgtcagtt agggtgtgga aagtccccag gctccccagc aggcagaagt atgcaaagca
    12421 tgcatctcaa ttagtcagca accaggtgtg gaaagtcccc aggctcccca gcaggcagaa
    12481 gtatgcaaag catgcatctc aattagtcag caaccatagt cccgccccta actccgccca
    12541 tcccgcccct aactccgccc agttccgccc attctccgcc ccatggctga ctaatttttt
    12601 ttatttatgc agaggccgag gccgcctctg cctctgagct attccagaag tagtgaggag
    12661 gcttttttgg aggcctaggc ttttgcaaaa agctcccggg agcttgtata tccattttcg
    12721 gatctgatca gcacgtgttg acaattaatc atcggcatag tatatcggca tagtataata
    12781 cgacaaggtg aggaactaaa ccatggccaa gttgaccagt gccgttccgg tgctcaccgc
    12841 gcgcgacgtc gccggagcgg tcgagttctg gaccgaccgg ctcgggttct cccgggactt
    12901 cgtggaggac gacttcgccg gtgtggtccg ggacgacgtg accctgttca tcagcgcggt
    12961 ccaggaccag gtggtgccgg acaacaccct ggcctgggtg tgggtgcgcg gcctggacga
    13021 gctgtacgcc gagtggtcgg aggtcgtgtc cacgaacttc cgggacgcct ccgggccggc
    13081 catgaccgag atcggcgagc agccgtgggg gcgggagttc gccctgcgcg acccggccgg
    13141 caactgcgtg cacttcgtgg ccgaggagca ggactgacac gtgctacgag atttcgattc
    13201 caccgccgcc ttctatgaaa ggttgggctt cggaatcgtt ttccgggacg ccggctggat
    13261 gatcctccag cgcggggatc tcatgctgga gttcttcgcc caccccaact tgtttattgc
    13321 agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata aagcattttt
    13381 ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttatc atgtctgtat
    13441 accgtcgacc tctagctaga gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa
    13501 ttgttatccg ctcacaattc cacacaacat acgagccgga agcataaagt gtaaagcctg
    13561 gggtgcctaa tgagtgagct aactcacatt aattgcgttg cgctcactgc ccgctttcca
    13621 gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg ggagaggcgg
    13681 tttgcgtatt gggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg
    13741 gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg
    13801 ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa
    13861 ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg
    13921 acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc
    13981 tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc
    14041 ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc
    14101 ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg
    14161 ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc
    14221 actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga
    14281 gttcttgaag tggtggccta actacggcta cactagaaga acagtatttg gtatctgcgc
    14341 tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac
    14401 caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg
    14461 atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc
    14521 acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa
    14581 ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta
    14641 ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt
    14701 tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag
    14761 tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag caataaacca
    14821 gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc
    14881 tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt
    14941 tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag
    15001 ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt
    15061 tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat
    15121 ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt
    15181 gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc
    15241 ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat
    15301 cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag
    15361 ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt
    15421 ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg
    15481 gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta
    15541 ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc
    15601 gcgcacattt ccccgaaaag tgccacctga c
//
