LOCUS       pgRNA_hMECP2_pro        8317 bp DNA     circular SYN 23-NOV-2022
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8317)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 4, 2022 from SnapGene 6.1.2
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..8317
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    15..317
                     /label=mU6 promoter (modified)
     misc_feature    257..273
                     /label=Sequencing Primer_SL-51
     misc_feature    318..329
                     /label=BstX I site
     misc_feature    348..440
                     /label=sgRNA scafold_93nt (F+E)
     misc_feature    441..446
                     /label=Xho I site
     misc_feature    1099..1695
                     /label=puro
     misc_feature    1234..1253
                     /label=qPCR For
     misc_feature    1419..1437
                     /label=qPCR rev
                     /label=qPCR rev(1)
     misc_feature    1702..1755
                     /label=t2A
     misc_feature    1771..2481
                     /label=mCherry
     misc_feature    2299..2318
                     /label=qPCR for
     misc_feature    2378..2397
                     /label=qPCR rev
ORIGIN
        1 gggcccgctc tagagatccg acgcgccatc tctaggcccg cgccggcccc ctcgcacaga
       61 cttgtgggag aagctcggct actcccctgc cccggttaat ttgcatataa tatttcctag
      121 taactataga ggcttaatgt gcgataaaag acagataatc tgttcttttt aatactagct
      181 acattttaca tgataggctt ggatttctat aacttcgtat agcatacatt atacgaagtt
      241 ataaacagca caaaaggaaa ctcaccctaa ctgtaaagta attgtgtgtt ttgagactat
      301 aagtatccct tggagaacca ccttgttggg ccatcacagc caatgacgtt taagagctat
      361 gctggaaaca gcatagcaag tttaaataag gctagtccgt tatcaacttg aaaaagtggc
      421 accgagtcgg tgcttttttt ctcgagtact aggatccatt aggcggccgc gtggataacc
      481 gtattaccgc catgcattag ttattaatag taatcaatta cggggtcatt agttcatagc
      541 ccatatatgg agttccgcgt tacataactt acggtaaatg gcccgcctgg ctgaccgccc
      601 aacgaccccc gcccattgac gtcaataatg acgtatgttc ccatagtaac gccaataggg
      661 actttccatt gacgtcaatg ggtggagtat ttacggtaaa ctgcccactt ggcagtacat
      721 caagtgtatc atatgccaag tacgccccct attgacgtca atgacggtaa atggcccgcc
      781 tggcattatg cccagtacat gaccttatgg gactttccta cttggcagta catctacgta
      841 ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt acatcaatgg gcgtggatag
      901 cggtttgact cacggggatt tccaagtctc caccccattg acgtcaatgg gagtttgttt
      961 tggcaccaaa atcaacggga ctttccaaaa tgtcgtaaca actccgcccc attgacgcaa
     1021 atgggcggta ggcgtgtacg gtgggaggtc tatataagca gagctggttt agtgaaccgt
     1081 cagatccgct agcccaccat gaccgagtac aagcccacgg tgcgcctcgc cacccgcgac
     1141 gacgtccccc gggccgtacg caccctcgcc gccgcgttcg ccgactaccc cgccacgcgc
     1201 cacaccgtcg acccggaccg ccacatcgag cgggtcaccg agctgcaaga actcttcctc
     1261 acgcgcgtcg ggctcgacat cggcaaggtg tgggtcgcgg acgacggcgc cgcggtggcg
     1321 gtctggacca cgccggagag cgtcgaagcg ggggcggtgt tcgccgagat cggcccgcgc
     1381 atggccgagt tgagcggttc ccggctggcc gcgcagcaac agatggaagg cctcctggcg
     1441 ccgcaccggc ccaaggagcc cgcgtggttc ctggccaccg tcggcgtctc gcccgaccac
     1501 cagggcaagg gtctgggcag cgccgtcgtg ctccccggag tggaggcggc cgagcgcgcc
     1561 ggggtgcccg ccttcctgga gacctccgcg ccccgcaacc tccccttcta cgagcggctc
     1621 ggcttcaccg tcaccgccga cgtcgaggtg cccgaaggac cgcgcacctg gtgcatgacc
     1681 cgcaagcccg gtgccggatc gggagagggc agaggaagtc tgctaacatg cggtgacgtc
     1741 gaggagaatc ctggcccacc ggtcgccacc atggtgagca agggcgagga ggataacatg
     1801 gccatcatca aggagttcat gcgcttcaag gtgcacatgg agggctccgt gaacggccac
     1861 gagttcgaga tcgagggcga gggcgagggc cgcccctacg agggcaccca gaccgccaag
     1921 ctgaaggtga ccaagggtgg ccccctgccc ttcgcctggg acatcctgtc ccctcagttc
     1981 atgtacggct ccaaggccta cgtgaagcac cccgccgaca tccccgacta cttgaagctg
     2041 tccttccccg agggcttcaa gtgggagcgc gtgatgaact tcgaggacgg cggcgtggtg
     2101 accgtgaccc aggactcctc cctgcaggac ggcgagttca tctacaaggt gaagctgcgc
     2161 ggcaccaact tcccctccga cggccccgta atgcagaaga agaccatggg ctgggaggcc
     2221 tcctccgagc ggatgtaccc cgaggacggc gccctgaagg gcgagatcaa gcagaggctg
     2281 aagctgaagg acggcggcca ctacgacgct gaggtcaaga ccacctacaa ggccaagaag
     2341 cccgtgcagc tgcccggcgc ctacaacgtc aacatcaagt tggacatcac ctcccacaac
     2401 gaggactaca ccatcgtgga acagtacgaa cgcgccgagg gccgccactc caccggcggc
     2461 atggacgagc tgtacaagta agaattcgtc gagggaccta ataacttcgt atagcataca
     2521 ttatacgaag ttatacatgt ttaagggttc cggttccact aggtacaatt cgatatcaag
     2581 cttatcgata atcaacctct ggattacaaa atttgtgaaa gattgactgg tattcttaac
     2641 tatgttgctc cttttacgct atgtggatac gctgctttaa tgcctttgta tcatgctatt
     2701 gcttcccgta tggctttcat tttctcctcc ttgtataaat cctggttgct gtctctttat
     2761 gaggagttgt ggcccgttgt caggcaacgt ggcgtggtgt gcactgtgtt tgctgacgca
     2821 acccccactg gttggggcat tgccaccacc tgtcagctcc tttccgggac tttcgctttc
     2881 cccctcccta ttgccacggc ggaactcatc gccgcctgcc ttgcccgctg ctggacaggg
     2941 gctcggctgt tgggcactga caattccgtg gtgttgtcgg ggaaatcatc gtcctttcct
     3001 tggctgctcg cctgtgttgc cacctggatt ctgcgcggga cgtccttctg ctacgtccct
     3061 tcggccctca atccagcgga ccttccttcc cgcggcctgc tgccggctct gcggcctctt
     3121 ccgcgtcttc gccttcgccc tcagacgagt cggatctccc tttgggccgc ctccccgcat
     3181 cgataccgtc gacctcgatc gagacctaga aaaacatgga gcaatcacaa gtagcaatac
     3241 agcagctacc aatgctgatt gtgcctggct agaagcacaa gaggaggagg aggtgggttt
     3301 tccagtcaca cctcaggtac ctttaagacc aatgacttac aaggcagctg tagatcttag
     3361 ccacttttta aaagaaaagg ggggactgga agggctaatt cactcccaac gaagacaaga
     3421 tatccttgat ctgtggatct accacacaca aggctacttc cctgattggc agaactacac
     3481 accagggcca gggatcagat atccactgac ctttggatgg tgctacaagc tagtaccagt
     3541 tgagcaagag aaggtagaag aagccaatga aggagagaac acccgcttgt tacaccctgt
     3601 gagcctgcat gggatggatg acccggagag agaagtatta gagtggaggt ttgacagccg
     3661 cctagcattt catcacatgg cccgagagct gcatccggac tgtactgggt ctctctggtt
     3721 agaccagatc tgagcctggg agctctctgg ctaactaggg aacccactgc ttaagcctca
     3781 ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa
     3841 ctagagatcc ctcagaccct tttagtcagt gtggaaaatc tctagcagca tgtgagcaaa
     3901 aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct
     3961 ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac
     4021 aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc
     4081 gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc
     4141 tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg
     4201 tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga
     4261 gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta acaggattag
     4321 cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta
     4381 cactagaaga acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag
     4441 agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg
     4501 caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac
     4561 ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc
     4621 aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag
     4681 tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc
     4741 agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac
     4801 gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc
     4861 accggctcca gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg
     4921 tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag
     4981 tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc
     5041 acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac
     5101 atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag
     5161 aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac
     5221 tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg
     5281 agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc
     5341 gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact
     5401 ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg
     5461 atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa
     5521 tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt
     5581 tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg
     5641 tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctga
     5701 cgcttaagcg gtcgacggat cgggagatct cccgatcccc tatggtgcac tctcagtaca
     5761 atctgctctg atgccgcata gttaagccag tatctgctcc ctgcttgtgt gttggaggtc
     5821 gctgagtagt gcgcgagcaa aatttaagct acaacaaggc aaggcttgac cgacaattgc
     5881 atgaagaatc tgcttagggt taggcgtttt gcgctgcttc gcgatgtacg ggccagatat
     5941 acgcgttgac attgattatt gactagttat taatagtaat caattacggg gtcattagtt
     6001 catagcccat atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga
     6061 ccgcccaacg acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca
     6121 atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca
     6181 gtacatcaag tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg
     6241 cccgcctggc attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc
     6301 tacgtattag tcatcgctat taccatggtg atgcggtttt ggcagtacat caatgggcgt
     6361 ggatagcggt ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt
     6421 ttgttttggc accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg
     6481 acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata taagcagcgc gttttgcctg
     6541 tactgggtct ctctggttag accagatctg agcctgggag ctctctggct aactagggaa
     6601 cccactgctt aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct
     6661 gttgtgtgac tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc
     6721 tagcagtggc gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga
     6781 cgcaggactc ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt
     6841 acgccaaaaa ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt
     6901 attaagcggg ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag
     6961 aaaaaatata aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt
     7021 aatcctggcc tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca
     7081 tcccttcaga caggatcaga agaacttaga tcattatata atacagtagc aaccctctat
     7141 tgtgtgcatc aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa
     7201 gagcaaaaca aaagtaagac caccgcacag caagcggccg gccgcgctga tcttcagacc
     7261 tggaggagga gatatgaggg acaattggag aagtgaatta tataaatata aagtagtaaa
     7321 aattgaacca ttaggagtag cacccaccaa ggcaaagaga agagtggtgc agagagaaaa
     7381 aagagcagtg ggaataggag ctttgttcct tgggttcttg ggagcagcag gaagcactat
     7441 gggcgcagcg tcaatgacgc tgacggtaca ggccagacaa ttattgtctg gtatagtgca
     7501 gcagcagaac aatttgctga gggctattga ggcgcaacag catctgttgc aactcacagt
     7561 ctggggcatc aagcagctcc aggcaagaat cctggctgtg gaaagatacc taaaggatca
     7621 acagctcctg gggatttggg gttgctctgg aaaactcatt tgcaccactg ctgtgccttg
     7681 gaatgctagt tggagtaata aatctctgga acagatttgg aatcacacga cctggatgga
     7741 gtgggacaga gaaattaaca attacacaag cttaatacac tccttaattg aagaatcgca
     7801 aaaccagcaa gaaaagaatg aacaagaatt attggaatta gataaatggg caagtttgtg
     7861 gaattggttt aacataacaa attggctgtg gtatataaaa ttattcataa tgatagtagg
     7921 aggcttggta ggtttaagaa tagtttttgc tgtactttct atagtgaata gagttaggca
     7981 gggatattca ccattatcgt ttcagaccca cctcccaacc ccgaggggac ccgacaggcc
     8041 cgaaggaata gaagaagaag gtggagagag agacagagac agatccattc gattagtgaa
     8101 cggatcggca ctgcgtgcgc caattctgca gacaaatggc agtattcatc cacaatttta
     8161 aaagaaaagg ggggattggg gggtacagtg caggggaaag aatagtagac ataatagcaa
     8221 cagacataca aactaaagaa ttacaaaaac aaattacaaa aattcaaaat tttcgggttt
     8281 attacaggga cagcagagat ccagtttggt tagtacc
//
