LOCUS       PE4max-Cas9-Plas       12576 bp DNA     circular SYN 31-MAR-2022
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  recombinant plasmid
REFERENCE   1  (bases 1 to 12576)
  AUTHORS   Roman Oliynyk
  TITLE     Direct Submission
  JOURNAL   Exported Nov 23, 2022 from SnapGene Viewer 6.1.2
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..12576
                     /mol_type="other DNA"
                     /organism="recombinant plasmid"
     enhancer        1..380
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
                     /note="color: #a6acb3"
     promoter        381..584
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /note="color: #ffffff"
     promoter        626..644
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
                     /note="color: #ffffff"
     CDS             658..714
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian
                     virus 40) large T antigen"
                     /label=SV40 NLS
                     /note="color: #993366"
                     /translation="MKRTADGSEFESPKKKRKV"
     CDS             715..4815
                     /codon_start=1
                     /product="nickase mutant of the Cas9 endonuclease from 
                     theStreptococcus pyogenes Type II CRISPR/Cas system"
                     /label=Cas9(R221K N394K H840A)
                     /note="generates RNA-guided single strand nicks in DNA
                     dueto the H840A mutation in the HNH catalytic domain"
                     /note="/Original_Translation=DKKYSIGLDIGTNSVGWAVITDEYKVPSKK
                     FKVLG 
                     NTDRHSIKKNLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVD 
                     DSFFHRLEESFLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLR 
                     LIYLALAHMIKFRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKA 
                     ILSARLSKSRRLENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSK 
                     DTYDDDLDNLLAQIGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYD 
                     EHHQDLTLLKALVRQQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMD 
                     GTEELLVKLNREDLLRKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEK 
                     ILTFRIPYYVGPLARGNSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDK 
                     NLPNEKVLPKHSLLYEYFTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRK 
                     VTVKQLKEDYFKKIECFDSVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDIL 
                     EDIVLTLTLFEDREMIEERLKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQ 
                     SGKTILDFLKSDGFANRNFMQLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPA 
                     IKKGILQTVKVVDELVKVMGRHKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKE 
                     LGSQILKEHPVENTQLQNEKLYLYYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLK 
                     DDSIDNKVLTRSDKNRGKSDNVPSEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERG 
                     GLSELDKAGFIKRQLVETRQITKHVAQILDSRMNTKYDENDKLIREVKVITLKSKLVS 
                     DFRKDFQFYKVREINNYHHAHDAYLNAVVGTALIKKYPKLESEFVYGDYKVYDVRKMI 
                     AKSEQEIGKATAKYFFYSNIMNFFKTEITLANGEIRKRPLIETNGETGEIVWDKGRDF 
                     ATVRKVLSMPQVNIVKKTEVQTGGFSKESILPKRNSDKLIARKKDWDPKKYGGFDSPT 
                     VAYSVLVVAKVEKGKSKKLKSVKELLGITIMERSSFEKNPIDFLEAKGYKEVKKDLII 
                     KLPKYSLFELENGRKRMLASAGELQKGNELALPSKYVNFLYLASHYEKLKGSPEDNEQ 
                     KQLFVEQHKHYLDEIIEQISEFSKRVILADANLDKVLSAYNKHRDKPIREQAENIIHL 
                     FTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLDATLIHQSITGLYETRIDLSQLGGD"
                     /note="/modified_by=chenp"
                     /note="color: #993366"
                     /translation="DKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRKLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLKREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     misc_feature    1372..1374
                     /label=R221K
                     /label=nonstandard type: misc
                     /note="/created_by=chenp"
                     /note="/modified_by=chenp"
                     /note="color: #a6acb3"
     misc_feature    1891..1893
                     /label=N394K
                     /label=nonstandard type: misc
                     /note="/created_by=chenp"
                     /note="color: #a6acb3"
     CDS             4816..4917
                     /label=SGGSx2-sv40NLS-SGGSx2
                     /note="/created_by=chenp"
                     /note="color: #993366; direction: RIGHT"
     CDS             4918..6948
                     /label=RT codon optimized Genscript
                     /note="/created_by=jessiedavis"
                     /note="color: #993366; direction: RIGHT"
     CDS             6961..7014
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian
                     virus 40) large T antigen"
                     /label=SV40 NLS
                     /note="/Original_Translation=KRTADGSEFEPKKKRKV"
                     /note="color: #993366"
                     /translation="KRTADGSEFESPKKKRKV"
     CDS             7024..7050
                     /label=c-Myc NLS
                     /note="/created_by=chenp"
                     /note="color: #993366; direction: RIGHT"
     CDS             7060..7116
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding 
                     separatepolypeptides."
                     /note="color: #993366"
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             7117..9362
                     /label=MLH1 del754-756 genscript codon
                     /note="/created_by=chenp"
                     /note="/modified_by=chenp"
                     /note="color: #993366; direction: RIGHT"
     misc_feature    9323..9362
                     /label=5' 40nt overlap
                     /note="5' overlap"
                     /note="color: #ccffcc"
     misc_feature    9333..9362
                     /label=5' homology 30nt
                     /note="color: #00ff00"
     misc_feature    9363..9372
                     /label=Restore front cut area
                     /note="color: #a6acb3"
     CDS             9382..9435
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /note="color: #cc99b2
                     Cleavage site after base 9432"
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             9436..9831
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /note="color: #993366"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     misc_feature    9832..9864
                     /label=Restore end cut area
                     /note="color: #a6acb3"
     misc_feature    9865..9904
                     /label=40nt overlap 3'
                     /note="color: #ccffcc"
     misc_feature    9865..9894
                     /label=homology 30nt 3'
                     /note="color: #00ff00"
     polyA_signal    9886..10110
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
                     /note="color: #a6acb3"
     protein_bind    10205..10221
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /note="color: #31849b"
     promoter        complement(10229..10259)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
                     /note="color: #ffffff; direction: LEFT"
     protein_bind    10274..10295
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the
                     presenceof cAMP."
                     /note="color: #31849b"
     rep_origin      complement(10583..11171)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
                     /note="color: #ffff00"
     CDS             complement(11342..12202)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin,
                     and related antibiotics"
                     /note="color: #993366"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(12203..12307)
                     /gene="bla"
                     /label=AmpR promoter
                     /note="color: #ffffff; direction: LEFT"
ORIGIN
        1 gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
       61 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
      121 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
      181 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
      241 aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
      301 ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat
      361 tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc
      421 ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt
      481 ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa
      541 tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctggttta gtgaaccgtc
      601 agatccgcta gagatccgcg gccgctaata cgactcacta tagggagagc cgccaccatg
      661 aaacggacag ccgacggaag cgagttcgag tcaccaaaga agaagcggaa agtcgacaag
      721 aagtacagca tcggcctgga catcggcacc aactctgtgg gctgggccgt gatcaccgac
      781 gagtacaagg tgcccagcaa gaaattcaag gtgctgggca acaccgaccg gcacagcatc
      841 aagaagaacc tgatcggagc cctgctgttc gacagcggcg aaacagccga ggccacccgg
      901 ctgaagagaa ccgccagaag aagatacacc agacggaaga accggatctg ctatctgcaa
      961 gagatcttca gcaacgagat ggccaaggtg gacgacagct tcttccacag actggaagag
     1021 tccttcctgg tggaagagga taagaagcac gagcggcacc ccatcttcgg caacatcgtg
     1081 gacgaggtgg cctaccacga gaagtacccc accatctacc acctgagaaa gaaactggtg
     1141 gacagcaccg acaaggccga cctgcggctg atctatctgg ccctggccca catgatcaag
     1201 ttccggggcc acttcctgat cgagggcgac ctgaaccccg acaacagcga cgtggacaag
     1261 ctgttcatcc agctggtgca gacctacaac cagctgttcg aggaaaaccc catcaacgcc
     1321 agcggcgtgg acgccaaggc catcctgtct gccagactga gcaagagcag aaagctggaa
     1381 aatctgatcg cccagctgcc cggcgagaag aagaatggcc tgttcggaaa cctgattgcc
     1441 ctgagcctgg gcctgacccc caacttcaag agcaacttcg acctggccga ggatgccaaa
     1501 ctgcagctga gcaaggacac ctacgacgac gacctggaca acctgctggc ccagatcggc
     1561 gaccagtacg ccgacctgtt tctggccgcc aagaacctgt ccgacgccat cctgctgagc
     1621 gacatcctga gagtgaacac cgagatcacc aaggcccccc tgagcgcctc tatgatcaag
     1681 agatacgacg agcaccacca ggacctgacc ctgctgaaag ctctcgtgcg gcagcagctg
     1741 cctgagaagt acaaagagat tttcttcgac cagagcaaga acggctacgc cggctacatt
     1801 gacggcggag ccagccagga agagttctac aagttcatca agcccatcct ggaaaagatg
     1861 gacggcaccg aggaactgct cgtgaagctg aagagagagg acctgctgcg gaagcagcgg
     1921 accttcgaca acggcagcat cccccaccag atccacctgg gagagctgca cgccattctg
     1981 cggcggcagg aagattttta cccattcctg aaggacaacc gggaaaagat cgagaagatc
     2041 ctgaccttcc gcatccccta ctacgtgggc cctctggcca ggggaaacag cagattcgcc
     2101 tggatgacca gaaagagcga ggaaaccatc accccctgga acttcgagga agtggtggac
     2161 aagggcgctt ccgcccagag cttcatcgag cggatgacca acttcgataa gaacctgccc
     2221 aacgagaagg tgctgcccaa gcacagcctg ctgtacgagt acttcaccgt gtataacgag
     2281 ctgaccaaag tgaaatacgt gaccgaggga atgagaaagc ccgccttcct gagcggcgag
     2341 cagaaaaagg ccatcgtgga cctgctgttc aagaccaacc ggaaagtgac cgtgaagcag
     2401 ctgaaagagg actacttcaa gaaaatcgag tgcttcgact ccgtggaaat ctccggcgtg
     2461 gaagatcggt tcaacgcctc cctgggcaca taccacgatc tgctgaaaat tatcaaggac
     2521 aaggacttcc tggacaatga ggaaaacgag gacattctgg aagatatcgt gctgaccctg
     2581 acactgtttg aggacagaga gatgatcgag gaacggctga aaacctatgc ccacctgttc
     2641 gacgacaaag tgatgaagca gctgaagcgg cggagataca ccggctgggg caggctgagc
     2701 cggaagctga tcaacggcat ccgggacaag cagtccggca agacaatcct ggatttcctg
     2761 aagtccgacg gcttcgccaa cagaaacttc atgcagctga tccacgacga cagcctgacc
     2821 tttaaagagg acatccagaa agcccaggtg tccggccagg gcgatagcct gcacgagcac
     2881 attgccaatc tggccggcag ccccgccatt aagaagggca tcctgcagac agtgaaggtg
     2941 gtggacgagc tcgtgaaagt gatgggccgg cacaagcccg agaacatcgt gatcgaaatg
     3001 gccagagaga accagaccac ccagaaggga cagaagaaca gccgcgagag aatgaagcgg
     3061 atcgaagagg gcatcaaaga gctgggcagc cagatcctga aagaacaccc cgtggaaaac
     3121 acccagctgc agaacgagaa gctgtacctg tactacctgc agaatgggcg ggatatgtac
     3181 gtggaccagg aactggacat caaccggctg tccgactacg atgtggacgc tatcgtgcct
     3241 cagagctttc tgaaggacga ctccatcgac aacaaggtgc tgaccagaag cgacaagaac
     3301 cggggcaaga gcgacaacgt gccctccgaa gaggtcgtga agaagatgaa gaactactgg
     3361 cggcagctgc tgaacgccaa gctgattacc cagagaaagt tcgacaatct gaccaaggcc
     3421 gagagaggcg gcctgagcga actggataag gccggcttca tcaagagaca gctggtggaa
     3481 acccggcaga tcacaaagca cgtggcacag atcctggact cccggatgaa cactaagtac
     3541 gacgagaatg acaagctgat ccgggaagtg aaagtgatca ccctgaagtc caagctggtg
     3601 tccgatttcc ggaaggattt ccagttttac aaagtgcgcg agatcaacaa ctaccaccac
     3661 gcccacgacg cctacctgaa cgccgtcgtg ggaaccgccc tgatcaaaaa gtaccctaag
     3721 ctggaaagcg agttcgtgta cggcgactac aaggtgtacg acgtgcggaa gatgatcgcc
     3781 aagagcgagc aggaaatcgg caaggctacc gccaagtact tcttctacag caacatcatg
     3841 aactttttca agaccgagat taccctggcc aacggcgaga tccggaagcg gcctctgatc
     3901 gagacaaacg gcgaaaccgg ggagatcgtg tgggataagg gccgggattt tgccaccgtg
     3961 cggaaagtgc tgagcatgcc ccaagtgaat atcgtgaaaa agaccgaggt gcagacaggc
     4021 ggcttcagca aagagtctat cctgcccaag aggaacagcg ataagctgat cgccagaaag
     4081 aaggactggg accctaagaa gtacggcggc ttcgacagcc ccaccgtggc ctattctgtg
     4141 ctggtggtgg ccaaagtgga aaagggcaag tccaagaaac tgaagagtgt gaaagagctg
     4201 ctggggatca ccatcatgga aagaagcagc ttcgagaaga atcccatcga ctttctggaa
     4261 gccaagggct acaaagaagt gaaaaaggac ctgatcatca agctgcctaa gtactccctg
     4321 ttcgagctgg aaaacggccg gaagagaatg ctggcctctg ccggcgaact gcagaaggga
     4381 aacgaactgg ccctgccctc caaatatgtg aacttcctgt acctggccag ccactatgag
     4441 aagctgaagg gctcccccga ggataatgag cagaaacagc tgtttgtgga acagcacaag
     4501 cactacctgg acgagatcat cgagcagatc agcgagttct ccaagagagt gatcctggcc
     4561 gacgctaatc tggacaaagt gctgtccgcc tacaacaagc accgggataa gcccatcaga
     4621 gagcaggccg agaatatcat ccacctgttt accctgacca atctgggagc ccctgccgcc
     4681 ttcaagtact ttgacaccac catcgaccgg aagaggtaca ccagcaccaa agaggtgctg
     4741 gacgccaccc tgatccacca gagcatcacc ggcctgtacg agacacggat cgacctgtct
     4801 cagctgggag gtgactccgg cggaagctct ggtggcagca agcggaccgc cgacggctct
     4861 gaattcgaga gccctaagaa gaaaagaaag gtgagcggag gctctagcgg cggaagcacc
     4921 ctgaacattg aagacgagta tagactgcat gaaacaagca aggaacccga cgtgtccctg
     4981 ggctccacct ggctgtccga ctttccccag gcctgggccg agacaggagg aatgggcctg
     5041 gccgtgcggc aggcacccct gatcatccct ctgaaggcca cctctacacc cgtgagcatc
     5101 aagcagtacc ctatgtctca ggaggccaga ctgggcatca agcctcacat ccagaggctg
     5161 ctggaccagg gcatcctggt gccatgccag agcccctgga acacaccact gctgcccgtg
     5221 aagaagccag gcaccaatga ctatagaccc gtgcaggatc tgagagaggt gaacaagagg
     5281 gtggaggata tccaccccac cgtgcccaac ccttacaatc tgctgtccgg cctgccccct
     5341 tctcaccagt ggtatacagt gctggacctg aaggatgcct tcttttgtct gagactgcac
     5401 cctaccagcc agccactgtt cgcctttgag tggagggacc ctgagatggg catctctggc
     5461 cagctgacct ggacacgcct gcctcagggc ttcaagaata gcccaacact gtttaacgag
     5521 gccctgcacc gcgacctggc agatttccgg atccagcacc cagatctgat cctgctgcag
     5581 tacgtggacg atctgctgct ggccgccacc agcgagctgg attgccagca gggaacacgc
     5641 gccctgctgc agaccctggg aaacctggga tatagggcat ccgccaagaa ggcccagatc
     5701 tgtcagaagc aggtgaagta cctgggctat ctgctgaagg agggccagag atggctgaca
     5761 gaggccagga aggagacagt gatgggccag ccaacaccca agaccccaag acagctgagg
     5821 gagttcctgg gcaaagcagg attttgcagg ctgttcatcc caggattcgc agagatggca
     5881 gcacctctgt acccactgac caagccgggc accctgttta attggggccc tgaccagcag
     5941 aaggcctatc aggagatcaa gcaggccctg ctgacagcac cagccctggg cctgccagac
     6001 ctgaccaagc ctttcgagct gtttgtggat gagaagcagg gctacgccaa gggcgtgctg
     6061 acccagaagc tgggaccatg gagacggccc gtggcctatc tgtccaagaa gctggaccca
     6121 gtggcagcag gatggccacc atgcctgagg atggtggcag caatcgccgt gctgacaaag
     6181 gatgccggca agctgaccat gggacagcca ctggtcatcc tggcaccaca cgcagtggag
     6241 gccctggtga agcagcctcc agatcgctgg ctgtctaacg cccggatgac acactaccag
     6301 gccctgctgc tggacaccga tcgcgtgcag tttggccctg tggtggccct gaatccagcc
     6361 accctgctgc ctctgccaga ggagggcctg cagcacaact gtctggacat cctggcagag
     6421 gcacacggaa caaggccaga cctgaccgat cagcccctgc ctgacgccga tcacacatgg
     6481 tataccgatg gaagctccct gctgcaggag ggccagagga aggcaggagc agcagtgacc
     6541 acagagacag aagtgatctg ggccaaggcc ctgccagcag gcacatccgc ccagcgggcc
     6601 gagctgatcg ccctgaccca ggccctgaag atggccgagg gcaagaagct gaacgtgtac
     6661 acagactcca gatatgcctt cgccaccgca cacatccacg gagagatcta caggcgccgg
     6721 ggctggctga cctctgaggg caaggagatc aagaacaagg atgagatcct ggccctgctg
     6781 aaggccctgt ttctgcccaa gcggctgagc atcatccact gtcctggaca ccagaaggga
     6841 cactccgccg aggcaagggg caatcggatg gccgaccagg ccgccagaaa ggctgctatt
     6901 actgaaactc ccgacacttc cactctgctg attgaaaact cctccccttc tggcggctca
     6961 aaaagaaccg ccgacggcag cgaattcgag tctcccaaga agaagaggaa agtcggctct
     7021 ggccctgccg ctaagagagt gaagctggac ggaagcggag ctactaactt cagcctgctg
     7081 aagcaggctg gagacgtgga ggagaaccct ggacctagct tcgttgctgg agtcatccgg
     7141 agactggacg agacagtggt gaacagaatt gccgccggcg aggtgatcca gagacctgcc
     7201 aatgcaataa aggagatgat cgagaactgt ctggacgcca agtccacaag cattcaggtg
     7261 atcgtgaagg agggcggact gaagctgatc cagatccaag acaacggcac aggcatcaga
     7321 aaggaagatc tggacatcgt gtgtgaacgg ttcaccacat ctaagctgca gtcttttgag
     7381 gatctggcct ctatcagtac ctacggcttc agaggcgagg ccctggccag catcagccac
     7441 gtggcccatg tgaccatcac caccaaaacc gccgacggca aatgcgctta tcgcgctagc
     7501 tacagcgacg gcaagctgaa agccccgcca aagccttgcg ccggcaacca gggtacacag
     7561 ataacagtgg aggatctgtt ctacaacatc gccacccgga gaaaggccct gaaaaatccc
     7621 agcgaggagt acggcaagat cctggaagtc gtcggcagat actccgtgca caacgccgga
     7681 atcagcttta gcgtaaagaa gcagggagaa accgtggccg atgtgcgcac cctgccaaat
     7741 gccagcaccg tggataacat cagaagcatt ttcggaaatg ccgtgtccag agaactgatc
     7801 gagatcggct gcgaagataa gaccctggct tttaagatga acggctacat cagcaacgcc
     7861 aattactctg tgaagaagtg catctttctt ctgttcatca accacagact ggtggaaagc
     7921 accagcctgc ggaaagccat cgagacagtg tacgccgcct acctgcctaa gaacacccac
     7981 cccttcctgt acctgagcct cgagatcagc cctcagaacg tggacgtcaa tgtgcatcct
     8041 acaaagcacg aggtgcactt cctgcacgag gaaagcatcc tggaaagagt gcagcagcac
     8101 attgagagca agctgctggg ctctaacagc agcagaatgt acttcacaca gaccctgctg
     8161 cctggcctgg ccggcccctc aggcgaaatg gttaagtcca caacctctct gacctcatcc
     8221 agcaccagcg gttcttccga taaggtgtac gcccaccaga tggtgcggac cgactctcgg
     8281 gagcagaagc tggacgcctt tctgcaacct ctgagcaaac ctctgagctc tcagcctcag
     8341 gccatcgtga ccgaggacaa gacagatatc tcctccggcc gtgccagaca gcaggacgaa
     8401 gaaatgctcg agctgccagc tcctgccgag gtggccgcca agaaccagag cctggaggga
     8461 gataccacaa agggcaccag cgaaatgagc gagaagcggg gccctacctc cagcaacccc
     8521 agaaaacggc accgggagga cagcgacgtg gaaatggtgg aggacgacag cagaaaggaa
     8581 atgacagccg cttgtacccc tagaagaaga atcatcaacc tgacctccgt gctgagcctg
     8641 caggaggaga tcaacgagca gggccacgag gtgctgagag agatgctgca caatcacagc
     8701 ttcgtgggct gcgtgaaccc tcaatgggcc ctggctcagc atcaaacaaa gctgtacctg
     8761 ctgaacacca ccaagctgag cgaagagctg ttctaccaga tcctcatcta cgacttcgcc
     8821 aacttcggcg tgctacgcct gagcgagccc gcccctctgt ttgacctggc catgctggct
     8881 ctggatagcc cagaaagcgg ctggacagaa gaggacggac ctaaagaggg gctggctgaa
     8941 tacatcgtgg agttcctgaa gaaaaaggcc gagatgctgg ccgactactt ttctctggaa
     9001 atcgacgagg aaggcaacct gatcggcctg cctctgctga tcgataacta cgtgcctccc
     9061 ctggaaggcc tgcccatctt catcctgaga ctggctacag aggtgaactg ggacgaggaa
     9121 aaggaatgct tcgagtctct gagcaaggag tgcgccatgt tctatagcat cagaaaacag
     9181 tacatctctg aagagagcac tctgtctggc cagcagagtg aagtgcccgg aagcatcccc
     9241 aacagctgga agtggaccgt ggaacacatc gtgtacaagg ccctgcggag ccacattctc
     9301 cctcctaagc acttcaccga ggacggcaac atcctgcagc tggccaacct gcccgacctt
     9361 tataaggttt tcggcagtgg agagggcaga ggaagtctgc taacatgcgg tgacgtcgag
     9421 gagaatcctg gcccaatggc caagcctttg tctcaagaag aatccaccct cattgaaaga
     9481 gcaacggcta caatcaacag catccccatc tctgaagact acagcgtcgc cagcgcagct
     9541 ctctctagcg acggccgcat cttcactggt gtcaatgtat atcattttac tgggggacct
     9601 tgtgcagaac tcgtggtgct gggcactgct gctgctgcgg cagctggcaa cctgacttgt
     9661 atcgtcgcga tcggaaatga gaacaggggc atcttgagcc cctgcggacg gtgccgacag
     9721 gtgcttctcg atctgcatcc tgggatcaaa gccatagtga aggacagtga tggacagccg
     9781 acggcagttg ggattcgtga attgctgccc tctggttatg tgtgggaggg ctaaccggtc
     9841 atcatcacca tcaccattga gtttaaaccc gctgatcagc ctcgactgtg ccttctagtt
     9901 gccagccatc tgttgtttgc ccctcccccg tgccttcctt gaccctggaa ggtgccactc
     9961 ccactgtcct ttcctaataa aatgagaaaa ttgcatcgca ttgtctgagt aggtgtcatt
    10021 ctattctggg gggtggggtg gggcaggaca gcaaggggga ggattgggaa gacaatagca
    10081 ggcatgctgg ggatgcggtg ggctctatgg cttctgaggc ggaaagaacc agctggggct
    10141 cgataccgtc gacctctagc tagagcttgg cgtaatcatg gtcatagctg tttcctgtgt
    10201 gaaattgtta tccgctcaca attccacaca acatacgagc cggaagcata aagtgtaaag
    10261 cctagggtgc ctaatgagtg agctaactca cattaattgc gttgcgctca ctgcccgctt
    10321 tccagtcggg aaacctgtcg tgccagctgc attaatgaat cggccaacgc gcggggagag
    10381 gcggtttgcg tattgggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg
    10441 ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat
    10501 caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta
    10561 aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa
    10621 atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc
    10681 cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt
    10741 ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca
    10801 gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg
    10861 accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat
    10921 cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta
    10981 cagagttctt gaagtggtgg cctaactacg gctacactag aagaacagta tttggtatct
    11041 gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac
    11101 aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa
    11161 aaggatctca agaagatcct ttgatctttt ctacggggtc tgacactcag tggaacgaaa
    11221 actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt
    11281 taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca
    11341 gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca
    11401 tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc
    11461 ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa
    11521 accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc
    11581 agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca
    11641 acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat
    11701 tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag
    11761 cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac
    11821 tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt
    11881 ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt
    11941 gctcttgccc ggcgtcaata cgggataata ccgcgccaca tagcagaact ttaaaagtgc
    12001 tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat
    12061 ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca
    12121 gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga
    12181 cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg
    12241 gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg
    12301 ttccgcgcac atttccccga aaagtgccac ctgacgtcga cggatcggga gatcgatctc
    12361 ccgatcccct agggtctact ctcagtacaa tctgctctga tgccgcatag ttaagccagt
    12421 atctgctccc tgcttgtgtg ttggaggtcg ctgagtagtg cgcgagcaaa atttaagcta
    12481 caacaaggca aggcttgacc gacaattgca tgaagaatct gcttagggtt aggcgttttg
    12541 cgctgcttcg cgatgtacgg gccagatata cgcgtt
//
