LOCUS       Exported                7939 bp ds-DNA     circular SYN 16-NOV-2022
DEFINITION  LifeAct-mRuby
            From: X Liang, et al. EMBO J. 2022.
            
            Full plasmid sequenced on 15 Nov 2022.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7939)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Thursday, Nov 24, 2022 from SnapGene 4.3.11
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..7939
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     N_region        complement(1..670)
                     /label=AmpR
                     /note="color: #ccffff; direction: LEFT"
     promoter        complement(671..775)
                     /gene="bla"
                     /label=AmpR promoter
                     /note="color: #ffffff; direction: LEFT"
     misc_feature    1458..1492
                     /label=5' PiggyBac site
                     /note="color: #ffcc00"
     enhancer        1810..2189
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
                     /note="color: #ffff00"
     protein_bind    3550..3574
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
                     /note="color: #00ffff; direction: RIGHT"
     mRNA            3592..3642
                     /label=LifeAct
                     /note="color: #00ff00; direction: RIGHT"
     misc_structure  3664..4344
                     /label=mRuby
                     /note="color: #ff0000; direction: RIGHT"
     protein_bind    complement(4349..4373)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
                     /note="color: #00ffff; direction: LEFT"
     mRNA            5094..5498
                     /label=BSD
                     /note="color: #00ffff; direction: RIGHT"
     misc_feature    6468..6526
                     /label=3' PiggyBac Site
                     /note="color: #ff9900"
     mRNA            complement(7750..7939)
                     /label=AmpR
                     /note="color: #ccffcc; direction: LEFT"
ORIGIN
        1 ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat
       61 tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc
      121 attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt
      181 tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaagcg gttagctcct
      241 tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg
      301 cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg
      361 agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg
      421 cgtcaatacg ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa
      481 aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt
      541 aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt
      601 gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt
      661 gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca
      721 tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat
      781 ttccccgaaa agtgccacct aaattgtaag cgttaatatt ttgttaaaat tcgcgttaaa
      841 tttttgttaa atcagctcat tttttaacca ataggccgaa atcggcaaaa tcccttataa
      901 atcaaaagaa tagaccgaga tagggttgag tgttgttcca gtttggaaca agagtccact
      961 attaaagaac gtggactcca acgtcaaagg gcgaaaaacc gtctatcagg gcgatggccc
     1021 actacgtgaa ccatcaccct aatcaagttt tttggggtcg aggtgccgta aagcactaaa
     1081 tcggaaccct aaagggagcc cccgatttag agcttgacgg ggaaagccgg cgaacgtggc
     1141 gagaaaggaa gggaagaaag cgaaaggagc gggcgctagg gcgctggcaa gtgtagcggt
     1201 cacgctgcgc gtaaccacca cacccgccgc gcttaatgcg ccgctacagg gcgcgtccca
     1261 ttcgccattc aggctgcgca actgttggga agggcgatcg gtgcgggcct cttcgctatt
     1321 acgccagctg gcgaaagggg gatgtgctgc aaggcgatta agttgggtaa cgccagggtt
     1381 ttcccagtca cgacgttgta aaacgacggc cagtgaattg taatacgact cactataggg
     1441 cgaattgggt accttaaccc tagaaagata gtctgcgtaa aattgacgca tgcattcttg
     1501 aaatattgct ctctctttct aaatagcgcg aatccgtcgc tgtgcattta ggacatctca
     1561 gtcgccgctt ggagctcccg tgaggcgtgc ttgtcaatgc ggtaagtgtc actgattttg
     1621 aactataacg accgcgtgag tcaaaatgac gcatgattat cttttacgtg acttttaaga
     1681 tttaactcat acgataatta tattgttatt tcatgttcta cttacgtgat aacttattat
     1741 atatatattt tcttgttata gatatcataa cttcgtataa tgtatgctat acgaagttat
     1801 gcggccctcg acattgatta ttgactagtt attaatagta atcaattacg gggtcattag
     1861 ttcatagccc atatatggag ttccgcgtta cataacttac ggtaaatggc ccgcctggct
     1921 gaccgcccaa cgacccccgc ccattgacgt caataatgac gtatgttccc atagtaacgc
     1981 caatagggac tttccattga cgtcaatggg tggagtattt acggtaaact gcccacttgg
     2041 cagtacatca agtgtatcat atgccaagta cgccccctat tgacgtcaat gacggtaaat
     2101 ggcccgcctg gcattatgcc cagtacatga ccttatggga ctttcctact tggcagtaca
     2161 tctacgtatt agtcatcgct attaccatgg tcgaggtgag ccccacgttc tgcttcactc
     2221 tccccatctc ccccccctcc ccacccccaa ttttgtattt atttattttt taattatttt
     2281 gtgcagcgat gggggcgggg gggggggggg gcgcgcgcca ggcggggcgg ggcggggcga
     2341 ggggcggggc ggggcgaggc ggagaggtgc ggcggcagcc aatcagagcg gcgcgctccg
     2401 aaagtttcct tttatggcga ggcggcggcg gcggcggccc tataaaaagc gaagcgcgcg
     2461 gcgggcggga gtcgctgcgc gctgccttcg ccccgtgccc cgctccgccg ccgcctcgcg
     2521 ccgcccgccc cggctctgac tgaccgcgtt actcccacag gtgagcgggc gggacggccc
     2581 ttctcctccg ggctgtaatt agcgcttggt ttaatgacgg cttgtttctt ttctgtggct
     2641 gcgtgaaagc cttgaggggc tccgggaggg ccctttgtgc ggggggagcg gctcgggggg
     2701 tgcgtgcgtg tgtgtgtgcg tggggagcgc cgcgtgcggc tccgcgctgc ccggcggctg
     2761 tgagcgctgc gggcgcggcg cggggctttg tgcgctccgc agtgtgcgcg aggggagcgc
     2821 ggccgggggc ggtgccccgc ggtgcggggg gggctgcgag gggaacaaag gctgcgtgcg
     2881 gggtgtgtgc gtgggggggg tgagcagggg gtgtgggcgc gtcggtcggg ctgcaacccc
     2941 cccctgcacc cccctccccg agttgctgag cacggcccgg cttcgggtgc ggggctccgt
     3001 acggggcgtg gcgcggggct cgccgtgccg ggcggggggt ggcggcaggt gggggtgccg
     3061 ggcggggcgg ggccgcctcg ggccggggag ggctcggggg aggggcgcgg cggcccccgg
     3121 agcgccggcg gctgtcgagg cgcggcgagc cgcagccatt gccttttatg gtaatcgtgc
     3181 gagagggcgc agggactttc tttgtcccaa atctgtgcgg agccgaaatc tgggaggcgc
     3241 cgccgcaccc cctctagcgg gcgcggggcg aagcggtgcg gcgccggcag gaaggaaatg
     3301 ggcggggagg gccttcgtgc gtcgccgcgc cgccgtcccc ttctccctct ccagcctcgg
     3361 ggctgtccgc ggggggacgg ctgccttcgg gggggacggg gcagggcggg gttcggcttc
     3421 tggcgtgtga ccggcggctc tagagcctct gctaaccatg ttcatgcctt cttctttttc
     3481 ctacagctcc tgggcaacgt gctggttatt gtgctgtctc atcattttgg caaagaattc
     3541 ctcgaatcaa caagtttgta caaaaaagca ggcttcgaag gagatagaac catgggcgtg
     3601 gccgacctga tcaagaagtt cgagagcatc agcaaggagg agccggatcc accggtcgcc
     3661 accatgaaca gcctgatcaa agaaaacatg cggatgaagg tggtgctgga aggcagcgtg
     3721 aacggccacc agttcaagtg caccggcgag ggcgagggca acccctacat gggcacccag
     3781 accatgcgga tcaaagtgat cgagggcgga cctctgccct tcgccttcga catcctggcc
     3841 acatccttca tgtacggcag ccggaccttc atcaagtacc ccaagggcat ccccgatttc
     3901 ttcaagcaga gcttccccga gggcttcacc tgggagagag tgaccagata cgaggacggc
     3961 ggcgtgatca ccgtgatgca ggacaccagc ctggaagatg gctgcctggt gtaccatgcc
     4021 caggtcaggg gcgtgaattt tcccagcaac ggcgccgtga tgcagaagaa aaccaagggc
     4081 tgggagccca acaccgagat gatgtacccc gctgacggcg gactgagagg ctacacccac
     4141 atggccctga aggtggacgg cggagggcac ctgagctgca gcttcgtgac cacctaccga
     4201 tccaagaaaa ccgtgggcaa catcaagatg cccggcatcc acgccgtgga ccaccggctg
     4261 gaaaggctgg aagagtccga caacgagatg ttcgtggtgc agcgggagca cgccgtggcc
     4321 aagttcgccg gcctgggcgg agggtaacac ccagctttct tgtacaaagt ggttgattcg
     4381 aggccgctcg ataagcttga tatcgaattc actcctcagg tgcaggctgc ctatcagaag
     4441 gtggtggctg gtgtggccaa ctggctcaca aataccactg agatcgacgg tatcgataag
     4501 cttgatatcg aattccgccc cccccctctc cctccccccc taacgttact ggccgaagcc
     4561 gcttggaata aggccggtgt gcgtttgtct atatgttatt ttccaccata ttgccgtctt
     4621 ttggcaatgt gagggcccgg aaacctggcc ctgtcttctt gacgagcatt cctaggggtc
     4681 tttcccctct cgccaaagga atgcaaggtc tgttgaatgt cgtgaaggaa gcagttcctc
     4741 tggaagcttc ttgaagacaa acaacgtctg tagcgaccct ttgcaggcag cggaaccccc
     4801 acctggcgac aggtgcctct gcggccaaaa gccacgtgta taagatacac ctgcaaaggc
     4861 ggcacaaccc cagtgccacg ttgtgagttg gatagttgtg gaaagagtca aatggctctc
     4921 ctcaagcgta ttcaacaagg ggctgaagga tgcccagaag gtaccccatt gtatgggatc
     4981 tgatctgggg cctcggtgca catgctttac atgtgtttag tcgaggttaa aaaaacgtct
     5041 aggccccccg aaccacgggg acgtggtttt cctttgaaaa acacgatgat aatatggcca
     5101 caaccatgcc tttgtctcaa gaagaatcca ccctcattga aagagcaacg gctacaatca
     5161 acagcatccc catctctgaa gactacagcg tcgccagcgc agctctctct agcgacggcc
     5221 gcatcttcac tggtgtcaat gtatatcatt ttactggggg accttgtgca gaactcgtgg
     5281 tgctgggcac tgctgctgct gcggcagctg gcaacctgac ttgtatcgtc gcgatcggaa
     5341 atgagaacag gggcatcttg agcccctgcg gacggtgccg acaggtgctt ctcgatctgc
     5401 atcctgggat caaagccata gtgaaggaca gtgatggaca gccgacggca gttgggattc
     5461 gtgaattgct gccctctggt tatgtgtggg agggttaact agataagtaa tgatcataat
     5521 cagccatatc acatctgtag aggttttact tgctttgtcc gacgcggccc gacgggtccg
     5581 agggggggtc gatctttttc cctctgccaa aaattatggg gacatcatga agccccttga
     5641 gcatctgact tctggctaat aaaggaaatt tattttcatt gcaatagtgt gttggaattt
     5701 tttgtgtctc tcactcggaa ggacatatgg gagggcaaat catttaaaac atcagaatga
     5761 gtatttggtt tagagtttgg caacatatgc ccatatgctg gctgccatga acaaaggttg
     5821 gctataaaga ggtcatcagt atatgaaaca gccccctgct gtccattcct tattccatag
     5881 aaaagccttg acttgaggtt agattttttt tatattttgt tttgtgttat tttttcttta
     5941 acatccctaa aattttcctt acatgtttta ctagccagat ttttcctcct ctcctgacta
     6001 ctcccagtca tagctgtccc tcttctctta tggagatccc tcgacctgca gcccaagctt
     6061 ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt tatccgctca caattccaca
     6121 caacatacga gccggaagca taaagtgtaa agcctggggt gcctaatgag tgagctaact
     6181 cacattaatt gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt cgtgccagcg
     6241 gatccgcggc cgcgtcgaga taacttcgta taatgtatgc tatacgaagt tatgttttgt
     6301 tactttatag aagaaatttt gagtttttgt ttttttttaa taaataaata aacataaata
     6361 aattgtttgt tgaatttatt attagtatgt aagtgtaaat ataataaaac ttaatatcta
     6421 ttcaaattaa taaataaacc tcgatataca gaccgataaa acacatgcgt caattttacg
     6481 catgattatc tttaacgtac gtcacaatat gattatcttt ctagggttaa agagctccag
     6541 cttttgttcc ctttagtgag ggttaatttc gagcttggcg taatcatggt catagctgtt
     6601 tcctgtgtga aattgttatc cgctcacaat tccacacaac atacgagccg gaagcataaa
     6661 gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca ttaattgcgt tgcgctcact
     6721 gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc
     6781 ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg
     6841 ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc
     6901 cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag
     6961 gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc tgacgagcat
     7021 cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag
     7081 gcgtttcccc ccggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga
     7141 tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg
     7201 tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt
     7261 cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac
     7321 gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc
     7381 ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag aacagtattt
     7441 ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc
     7501 ggcaaacaaa ccaccgctgg tagcggtggt tttttttgtt tgcaagcagc agattacgcg
     7561 cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg
     7621 gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta
     7681 gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg
     7741 gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg
     7801 ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc
     7861 atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc
     7921 agcaataaac cagccagcc
//
