LOCUS       Exported                9485 bp ds-DNA     circular SYN 16-AUG-2022
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    pBID2_QUAS_MCS
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9485)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Monday, Nov 28, 2022 from SnapGene 5.0.8
            https://www.snapgene.com
COMMENT     pBID-UAS-SynR from 1 to 9774
FEATURES             Location/Qualifiers
     source          1..9485
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     rep_origin      complement(135..441)
                     /direction=LEFT
                     /label=F1 ori
     misc_feature    667..4785
                     /label=Mini-White
     misc_feature    4838..5268
                     /label=Gypsy Ins.
                     /label=Gypsy Insulator
     promoter        5314..5501
                     /label=10xQUAS
     promoter        5528..5682
                     /label=DSCP
     misc_feature    5683..5744
                     /label=MCS
     misc_feature    5745..6411
                     /label=p10 3'UTR
     misc_feature    6545..6975
                     /label=Gypsy Ins.
                     /label=Gypsy Insulator(1)
     misc_recomb     6988..7272
                     /label=attB
                     /label=attB PhiC Integrase
     rep_origin      complement(7720..8402)
                     /direction=LEFT
                     /label=ColE1 origin
     CDS             complement(8500..9159)
                     /label=AmpR
ORIGIN
        1 ctaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc
       61 attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga
      121 gatagggttg agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc
      181 caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg aaccatcacc
      241 ctaatcaagt tttttggggt cgaggtgccg taaagcacta aatcggaacc ctaaagggag
      301 cccccgattt agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa
      361 agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac
      421 cacacccgcc gcgcttaatg cgccgctaca gggcgcgtcc cattcgccat tcaggctgcg
      481 caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg
      541 gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg
      601 taaaacgacg gccagtgaat tgtaatacga ctcactatag ggcgaattgg gtacgtaccg
      661 ggcccctagt atgtatgtaa gttaataaaa cccatttttg cggaaagtag ataaaaaaaa
      721 catttttttt ttttactgca ctggatatca ttgaacttat ctgatcagtt ttaaatttac
      781 ttcgatccaa gggtatttga tgtaccaggt tctttcgatt acctctcact caaaatgaca
      841 ttccactcaa agtcagcgct gtttgcctcc ttctctgtcc acagaaatat cgccgtctct
      901 ttcgccgctg cgtccgctat ctctttcgcc accgtttgta gcgttacgta gcgtcaatgt
      961 ccgccttcag ttgcattttg tcagcggttt cgtgacgaag ctccaagcgg tttacgccat
     1021 caattaaaca caaagtgctg tgccaaaact cctctcgctt cttatttttg tttgtttttt
     1081 gagtgattgg ggtggtgatt ggttttgggt gggtaagcag gggaaagtgt gaaaaatccc
     1141 ggcaatgggc caagaggatc aggagctatt aattcgcgga ggcagcaaac acccatctgc
     1201 cgagcatctg aacaatgtga gtagtacatg tgcatacatc ttaagttcac ttgatctata
     1261 ggaactgcga ttgcaacatc aaattgtctg cggcgtgaga actgcgaccc acaaaaatcc
     1321 caaaccgcaa ttgcacaaac aaatagtgac acgaaacaga ttattctggt agctgttctc
     1381 gctatataag acaatttttg agatcatatc atgatcaaga catctaaagg cattcatttt
     1441 cgactatatt cttttttaca aaaaatataa caaccagata ttttaagctg atcctagatg
     1501 cacaaaaaat aaataaaagt ataaacctac ttcgtaggat acttcggggt actttttgtt
     1561 cggggttaga tgagcataac gcttgtagtt gatatttgag atcccctatc attgcagggt
     1621 gacagcggag cggcttcgca gagctgcatt aaccagggct tcgggcaggc caaaaactac
     1681 ggcacgctcc ggccacccag tccgccggag gactccggtt cagggagcgg ccaactagcc
     1741 gagaacctca cctatgcctg gcacaatatg gacatctttg gggcggtcaa tcagccgggc
     1801 tccggatggc ggcagctggt caaccggaca cgcggactat tctgcaacga gcgacacata
     1861 ccggcgccca ggaaacattt gctcaagaac ggtgagtttc tattcgcagt cggctgatct
     1921 gtgtgaaatc ttaataaagg gtccaattac caatttgaaa ctcagtttgc ggcgtggcct
     1981 atccgggcga acttttggcc gtgatgggca gttccggtgc cggaaagacg accctgctga
     2041 atgcccttgc ctttcgatcg ccgcagggca tccaagtatc gccatccggg atgcgactgc
     2101 tcaatggcca acctgtggac gccaaggaga tgcaggccag gtgcgcctat gtccagcagg
     2161 atgacctctt tatcggctcc ctaacggcca gggaacacct gattttccaa gccatggtgc
     2221 ggatgccacg acatctgacc tatcggcagc gagtggcccg cgtggatcag gtgatccagg
     2281 agctttcgct cagcaaatgt cagcacacga tcatcggtgt gcccggcagg gtgaaaggtc
     2341 tgtccggcgg agaaaggaag cgtctggcat tcgcctccga ggctctaacc gatccgccgc
     2401 ttctgatctg cgatgagccc acctccggac tggactcctt taccgcccac agcgtcgtcc
     2461 aggtgctgaa gaagctgtcg cagaagggca agaccgtcat cctgaccatt catcagccgt
     2521 cttccgagct gtttgagctc tttgacaaga tccttctgat ggccgagggc agggtagctt
     2581 tcttgggcac tcccagcgaa gccgtcgact tcttttccta gtgagttcga tgtgtttatt
     2641 aagggtatct agtattacat aacatctcaa ctcctatcca gcgtgggtgc ccagtgtcct
     2701 accaactaca atccggcgga cttttacgta caggtgttgg ccgttgtgcc cggacgggag
     2761 atcgagtccc gtgatcggat cgccaagata tgcgacaatt ttgccattag caaagtagcc
     2821 cgggatatgg agcagttgtt ggccaccaaa aatctggaga agccactgga gcagccggag
     2881 aatgggtaca cctacaaggc cacctggttc atgcagttcc gggcggtcct gtggcgatcc
     2941 tggctgtcgg tgctcaagga accactcctc gtaaaagtgc gacttattca gacaacggtg
     3001 agtggttcca gtggaaacaa atgatataac gcttacaatt cttggaaaca aattcgctag
     3061 attttagata gaattgcctg attccacacc cttcttagtt tttttcaatg agatgtatag
     3121 tttatagttt tgcagaagat aaataaattt catttaactc gcgaatatta atgagatgcg
     3181 agtaacattt taatttgcag atggttgcca tcttgattgg cctcatcttt ttgggccaac
     3241 aactcacgca agtgggtgtg atgaatatca acggagccat cttcctcttc ctgaccaaca
     3301 tgacctttca aaacgtcttt gccacgataa atgtaagtca tgtttagaat acatttgcat
     3361 tccaataatt tactaacttt ctaatgaatc gattcgattt aggtgttcac ctcagagctg
     3421 ccagttttta tgagggaggc ccgaagtcga ctttatcgct gtgacacata ctttctgggc
     3481 aaaacgattg ccgaattgcc gctttttctc acagtgccac tggtcttcac ggcgattgcc
     3541 tatccgatga tcggactgcg ggccggagtg ctgcacttct tcaactgcct ggcgctggtc
     3601 actctggtgg ccaatgtgtc aacgtccttc ggatatctaa tatcctgcgc cagctcctcg
     3661 acctcgatgg cgctgtctgt gggtccgccg gttatcatac cattcctgct ctttggcggc
     3721 ttcttcttga actcgggctc ggtgccagta tacctcaaat ggttgtcgta cctctcatgg
     3781 ttccgttacg ccaacgaggg tctgctgatt aaccaatggg cggacgtgga gccgggcgaa
     3841 attagctgca catcgtcgaa caccacgtgc cccagttcgg gcaaggtcat cctggagacg
     3901 cttaacttct ccgccgccga tctgccgctg gactacgtgg gtctggccat tctcatcgtg
     3961 agcttccggg tgctcgcata tctggctcta agacttcggg cccgacgcaa ggagtagccg
     4021 acatatatcc gaaataactg cttgtttttt ttttttacca ttattaccat cgtgtttact
     4081 gtttattgcc ccctcaaaaa gctaatgtaa ttatatttgt gccaataaaa acaagatatg
     4141 acctatagaa tacaagtatt tccccttcga acatccccac aagtagactt tggatttgtc
     4201 ttctaaccaa aagacttaca cacctgcata ccttacatca aaaactcgtt tatcgctaca
     4261 taaaacaccg ggatatattt tttatataca tacttttcaa atcgcgcgcc ctcttcataa
     4321 ttcacctcca ccacaccacg tttcgtagtt gctctttcgc tgtctcccac ccgctctccg
     4381 caacacattc accttttgtt cgacgacctt ggagcgactg tcgttagttc cgcgcgattc
     4441 ggttcgctca aatggttccg agtggttcat ttcgtctcaa tagaaattag taataaatat
     4501 ttgtatgtac aatttatttg ctccaatata tttgtatata tttccctcac agctatattt
     4561 attctaattt aatattatga ctttttaagg taattttttg tgacctgttc ggagtgatta
     4621 gcgttacaat ttgaactgaa agtgacatcc agtgtttgtt ccttgtgtag atgcatctca
     4681 aaaaaatggt gggcataata gtgttgttta tatatatcaa aaataacaac tataataata
     4741 agaatacatt taatttagaa aatgcttgga tttcactgga actaggctag cataacttcg
     4801 tataatgtat gctatacgaa gttatgctag cggatcctgg ccacgtaata agtgtgcgtt
     4861 gaatttattc gcaaaaacat tgcatatttt cggcaaagta aaattttgtt gcatacctta
     4921 tcaaaaaata agtgctgcat actttttaga gaaaccaaat aattttttat tgcatacccg
     4981 tttttaataa aatacattgc ataccctctt ttaataaaaa atattgcata ctttgacgaa
     5041 acaaattttc gttgcatacc caataaaaga ttattatatt gcatacccgt ttttaataaa
     5101 atacattgca taccctcttt taataaaaaa tattgcatac gttgacgaaa caaattttcg
     5161 ttgcataccc aataaaagat tattatattg catacctttt cttgccatac catttagccg
     5221 atcaattgtg ctcggcaaca gtatatttgt ggtgtgccaa ccaacaacag atccaagctg
     5281 gccgcggctc gataagcttg tcactgggtc agtgggtaat cgcttatcct cggataaaca
     5341 attatcctca cgggtaatcg cttatccgct cgggtaatcg cttatcctcg ggtaatcgct
     5401 tatccttggg taatcgctta tcctcggata aacaattatc ctcacgggta atcgcttatc
     5461 cgctcgggta atcgcttatc ctcgggtaat cgcttatcct ttcacgttgg gacgtcgaga
     5521 ctctagcgag ctcgcccggg gatcgagcgc agcggtataa aagggcgcgg ggtggctgag
     5581 agcatcagtt gtgaatgaat gttcgagccg agcagacgtg ccgctgcctt cgttaatatc
     5641 ctttgaataa gccaactttg aatcacaaga cgcataccaa acgaattcgc agatcttaat
     5701 taaccggtac cngatatcgc atgcggccgc ggcgcgcctc tagaaatgaa tcgtttttaa
     5761 aataacaaat caattgtttt ataatattcg tacgattctt tgattatgta ataaaatgtg
     5821 atcattagga agattacgaa aaatataaaa aatatgagtt ctgtgtgtat aacaaatgct
     5881 gtaaacgcca caattgtgtt tgttgcaaat aaacccatga ttatttgatt aaaattgttg
     5941 ttttctttgt tcatagacaa tagtgtgttt tgcctaaacg tgtactgcat aaactccatg
     6001 cgagtgtata gcgagctagt ggctaacgct tgccccacca aagtagattc gtcaaaatcc
     6061 tcaatttcat caccctcctc caagtttaac atttggccgt cggaattaac ttctaaagat
     6121 gccacataat ctaataaatg aaatagagat tcaaacgtgg cgtcatcgtc cgtttcgacc
     6181 atttccgaaa agaactcggg cataaactct atgatttctc tggacgtggt gttgtcgaaa
     6241 ctctcaaagt acgcagtcag gaacgtgcgc gacatgtcgt cgggaaactc gcgcggaaac
     6301 atgttgttgt aaccgaacgg gtcccatagc gccaaaacca aatctgccag cgtcaataga
     6361 atgagcacga tgccgacaat ggagctggct tggatagcga ttcgagttaa cttgtttatt
     6421 gcagcttata atggttacaa ataaagcaat agcatcacaa atttcacaaa taaagcattt
     6481 ttttcactgc attctagttg tggtttgtcc aaactcatca atgtatctta tcatgtctgg
     6541 atcttggcca cgtaataagt gtgcgttgaa tttattcgca aaaacattgc atattttcgg
     6601 caaagtaaaa ttttgttgca taccttatca aaaaataagt gctgcatact ttttagagaa
     6661 accaaataat tttttattgc atacccgttt ttaataaaat acattgcata ccctctttta
     6721 ataaaaaata ttgcatactt tgacgaaaca aattttcgtt gcatacccaa taaaagatta
     6781 ttatattgca tacccgtttt taataaaata cattgcatac cctcttttaa taaaaaatat
     6841 tgcatacgtt gacgaaacaa attttcgttg catacccaat aaaagattat tatattgcat
     6901 accttttctt gccataccat ttagccgatc aattgtgctc ggcaacagta tatttgtggt
     6961 gtgccaacca acaacggatc cactagtgtc gacgatgtag gtcacggtct cgaagccgcg
     7021 gtgcgggtgc cagggcgtgc ccttgggctc cccgggcgcg tactccacct cacccatctg
     7081 gtccatcatg atgaacgggt cgaggtggcg gtagttgatc ccggcgaacg cgcggcgcac
     7141 cgggaagccc tcgccctcga aaccgctggg cgcggtggtc acggtgagca cgggacgtgc
     7201 gacggcgtcg gcgggtgcgg atacgcgggg cagcgtcagc gggttctcga cggtcacggc
     7261 gggcatgtcg acactagttc tagccagctt ttgttccctt tagtgagggt taatttcgag
     7321 cttggcgtaa tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc
     7381 acacaacata cgagccggaa gcataaagtg taaagcctgg ggtgcctaat gagtgagcta
     7441 actcacatta attgcgttgc gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca
     7501 gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc
     7561 cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc
     7621 tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat
     7681 gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt
     7741 ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg
     7801 aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc
     7861 tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt
     7921 ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa
     7981 gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta
     8041 tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa
     8101 caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa
     8161 ctacggctac actagaagaa cagtatttgg tatctgcgct ctgctgaagc cagttacctt
     8221 cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt
     8281 ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat
     8341 cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat
     8401 gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc
     8461 aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc
     8521 acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta
     8581 gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga
     8641 cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg
     8701 cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc
     8761 tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacaggcat
     8821 cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag
     8881 gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat
     8941 cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa
     9001 ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa
     9061 gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caatacggga
     9121 taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg
     9181 gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc
     9241 acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg
     9301 aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact
     9361 cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat
     9421 atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt
     9481 gccac
//
