LOCUS       Exported               13952 bp DNA     circular SYN 19-JAN-2023
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  recombinant plasmid
REFERENCE   1  (bases 1 to 13952)
  AUTHORS   knighkaz
  TITLE     Direct Submission
  JOURNAL   Exported Jan 19, 2023 from SnapGene 6.0.2
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..13952
                     /mol_type="other DNA"
                     /organism="recombinant plasmid"
     misc_feature    7..1342
                     /locus_tag="ttTi5605 left recomb "
                     /label=ttTi5605 left recomb
                     
                     /note="color: #8080c0"
     promoter        1343..2330
                     /locus_tag="Cb-unc-119 promoter"
                     /label=Cb-unc-119 promoter
                     /note="color: #3920ff"
     CDS             join(2331..2349,2351..3176)
                     /locus_tag="CB-unc-119(+)"
                     /label=CB-unc-119(+)
                     /note="This forward directional feature has 2 segments:
                        1: 2331 .. 2349 / #008000
                        2: 2351 .. 3176 / #008000"
     modified_base   complement(2350)
                     /locus_tag="mutation. Adds extra PvuI site"
                     /label=mutation. Adds extra PvuI site
                     /note="color: #00ff00; direction: LEFT"
     3'UTR           3177..3460
                     /locus_tag="Cb-unc-119 3'UTR"
                     /label=Cb-unc-119 3'UTR
                     /note="color: #c5ff29"
     misc_recomb     3568..3574
                     /locus_tag="attR4"
                     /label=attR4
                     /note="color: #ff80ff"
     promoter        3613..7776
                     /locus_tag="Pflp-18"
                     /label=Pflp-18
                     /note="color: #107ecf"
     misc_feature    7639..8669
                     /label=OFH 416  Post-Cloning Sequencing Confirmation
                     /note="color: #a6acb3"
     primer_bind     complement(7788..7808)
                     /label=Vector.REV
                     /note="color: black; sequence: GTCATGTCTAACCCTGAAATT; 
                     added: 2023-01-19"
     primer_bind     7794..7829
                     /label=Fragment.FOR
                     /note="color: black; sequence: 
                     AGGGTTAGACATGACATGAGCGTGCTGACACCTCTG; added: 2023-01-19"
     misc_feature    7808
                     /locus_tag="attP1R"
                     /label=attP1R
                     /note="color: #00ffff"
     CDS             7809..7883
                     /codon_start=1
                     /product="mitochondrial presequence of human cytochrome c 
                     oxidase subunit VIII (Rizutto et al., 1989)"
                     /label=COX8 presequence
                     /note="color: #993366
                     Cleavage site after base 7883"
                     /translation="MSVLTPLLLRGLTGSARRLPVPRAK"
     CDS             7905..7979
                     /codon_start=1
                     /product="mitochondrial presequence of human cytochrome c 
                     oxidase subunit VIII (Rizutto et al., 1989)"
                     /label=COX8 presequence
                     /note="color: #993366
                     Cleavage site after base 7979"
                     /translation="MSVLTPLLLRGLTGSARRLPVPRAK"
     CDS             8001..8075
                     /codon_start=1
                     /product="mitochondrial presequence of human cytochrome c 
                     oxidase subunit VIII (Rizutto et al., 1989)"
                     /label=COX8 presequence
                     /note="color: #993366
                     Cleavage site after base 8075"
                     /translation="MSVLTPLLLRGLTGSARRLPVPRAK"
     CDS             8097..8171
                     /codon_start=1
                     /product="mitochondrial presequence of human cytochrome c 
                     oxidase subunit VIII (Rizutto et al., 1989)"
                     /label=COX8 presequence
                     /note="color: #993366
                     Cleavage site after base 8171"
                     /translation="MSVLTPLLLRGLTGSARRLPVPRAK"
     CDS             8229..8246
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /note="color: #cc99b2"
                     /translation="HHHHHH"
     CDS             8286..8309
                     /codon_start=1
                     /product="Xpress(TM) epitope tag, including an enterokinase
                     recognition and cleavage site"
                     /label=Xpress(TM) tag
                     /note="This feature has 2 segments:
                        1: 8286 .. 8294 / #cc99b2
                        2: 8295 .. 8309 / #cc99b2 / enterokinase site
                     Cleavage site after base 8309"
                     /translation="DLYDDDDK"
     CDS             8337..8393
                     /codon_start=1
                     /product="calmodulin-binding peptide from avian smooth 
                     muscle myosin light chain kinase"
                     /label=calmodulin-binding peptide
                     /note="color: #cc99b2"
                     /translation="RRKWNKTGHAVRAIGRLSS"
     primer_bind     complement(8994..9013)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
                     /note="color: black; sequence: GTCTTGTAGTTGCCGTCGTC"
     primer_bind     9616..9637
                     /label=Vector.FOR
                     /note="color: black; sequence: CTTGTACAAAGTGGTTGGATGA; 
                     added: 2023-01-19"
     misc_feature    9632..10070
                     /locus_tag="3' UTR Let858"
                     /label=3' UTR Let858
                     /note="color: #00ffff"
     misc_feature    9632..9648
                     /locus_tag="Forward 3' UTR let858"
                     /label=Forward 3' UTR let858
                     /note="color: #00ff00"
     misc_feature    10051..10070
                     /locus_tag="Reverse 3' UTR let858"
                     /label=Reverse 3' UTR let858
                     /note="color: #00ff00"
     misc_recomb     complement(10079..10092)
                     /locus_tag="attR3"
                     /label=attR3
                     /note="color: #ff80ff; direction: LEFT"
     primer_bind     complement(10100..10117)
                     /locus_tag="M13-fwd"
                     /label=M13-fwd
                     /note="color: #00ff00; direction: LEFT"
     misc_recomb     10177..11604
                     /locus_tag="ttTi5605 right recomb"
                     /label=ttTi5605 right recomb
                     /note="color: #8080c0"
     CDS             12386..13045
                     /locus_tag="AmpR"
                     /label=AmpR
                     /note="color: #ffff00; direction: RIGHT"
     rep_origin      13143..13825
                     /locus_tag="ColE1 origin"
                     /label=ColE1 origin
                     /note="color: #808080"
ORIGIN
        1 gggccctgtc ggattatggg ctcttctttt atcctctact ttctttctag aaaatgcaaa
       61 tgttgagtca gaaattgctg aatggagtac aactgcaatg ggtttagctt atgtttctgg
      121 aacaattact ggtggaattg tgattgaaaa gtaaggttat atttcatttc aataaatgtt
      181 accacagtat aacaaaaaca agttacattg ttcagagtat gtctgaatgt attattatta
      241 ttgtatttgt gcatcagtac tgtagttgta ctgaagaagg gtttaaaata atctcaacgt
      301 ttttaagacg agttagtgag aatacggtcg cagttaggat gtttggacat agttttatta
      361 gataatcgtg aactaactca ctataaaaac ccgctaactc attgaataag atagttttaa
      421 acgttcaaaa tttgaagaca aaattgtatg aataagttta cagagttggt cgacgcaagc
      481 ttctccttct cttcacattt ctcaataatt tagctctaat tgcattcgta ttcttcgcaa
      541 aaatacgtat tctgattgac ccgatgaaat acggatgcct agttgcattg atcatctacg
      601 gttatactta tgggtgaggt ttttcaaata tattttgatt taatcgaata atgaaatttc
      661 agaactggag ttggtccaat ttcgtggttt atatcctctg aactggtacc tcaaaaacat
      721 cgtagtattg ctcaatctgt ggcatattca atgaatactg taatggtggt tatcagtaca
      781 tttacagtat tacctctata ttccgttatt ggtagttatg ctttccttat tctatattca
      841 attccttcat ttataagtat gcttatatta tttcgctact taccggaaac caaaggacga
      901 gagattcatg atattgtgaa tgaacttaaa aaaaaatgaa aacctttgaa aacgttcata
      961 cggccgaaat tttacttgat cgagccaatt gaacttttgt taagacttac agaaaagtga
     1021 gagattttgt tacaaaaaag tgagaaaaaa gcgaaaatga acaggaaaaa gaagaaaaat
     1081 acacccgaag tatcatataa aaattaaaaa gattgtttga cctggcggaa cttttttgca
     1141 aaaactctga atatccatgg cacatgcttc gtgcaaaaca ggatccaaat gaatatcaac
     1201 aagagattct tgaagacgac gagccacttg ctcagagcat tgcttatctc gaatgagacc
     1261 cttgttaaaa tcatttttca aacattatat gacattgtcg aaatgtcctc ctgattccat
     1321 gatggtagca aactcacttc gtctttgagc caatttatcc aagtccttgt aaaaagtatt
     1381 cgaaaattgt taacggataa aatgtttatt ataatatcaa aaacaatttg tcagttgacc
     1441 actttttgat atatttgaca gaaaccggga tgaattggct caaagttagg gcgcccttct
     1501 attacagggt ttctgataac aaaccggtta ttaactccca acaagggatg atttcaattc
     1561 atcatgctca aattgaccca aattaagtta catgacaaat tcatcgccct tttcaactct
     1621 tcttggctca tcatctgtta ttctgttcct attctgtctg caccctatac ccctttgcat
     1681 actctctcgt cattcctttc ttggatagtg tcttcttctc cccagctctg ctacttctat
     1741 gacttgcgcc cgctgctttt ccgctcgttc ctctcctctc tgacgtcttc ttctgcttct
     1801 tttctccttc ctttttccct tctcaatctt cctctttttc ccatttctgt caatcattcg
     1861 aagaagaaga agaagaagac cctcattcat ttcatttttt tttctgtcgg tgtgtgctgc
     1921 tcggttaaga gtgagctctc tattccacgt cttcttcttt ttcttcttcg attcgaatca
     1981 atcactccac caaaacgcat tcgtttttgg gattcacccc gcggttcgca ataggtttct
     2041 ttttcaaatt attagcgtta taaatagaaa aatggtggag tttcaataaa aatgataatt
     2101 tacaaagtgt attttgatta catgtactca aaaaggttga aatttttcat accagttttc
     2161 cggaaatcca tctgatatca ttatcgtatt tttcttttta aaaatgtttt caaaaaaaaa
     2221 caaaatatag ctggttattt ggcaccctct aattaccatt ttcctgtcac accacactct
     2281 tttccttctt ccacttcttt ccgttttcag ccgcttccaa ccaaaccgat atgaaagccg
     2341 agcaacaacg atcgattcca cccggctcgg cgaccttccc gtcgcaggtg agactcagaa
     2401 aactagagaa accgctcaac taactcttga tatccgattt cattcttttc tcttttcttt
     2461 ttgttgaact tttccatatt tccagatgcc acggccacca ccaagcaccg aacaaggaat
     2521 cacaacggaa tcggagcttg cgaagaaagc tcaaatcact ccgaacgacg ttttagcact
     2581 tccgggaatc actcaaggta tcttgctccc tttggatttc agtttaaaac ataaatttag
     2641 gattcttatg ctccccatct gcgaacatct ataacatcga gttcaccaag ttccaaatcc
     2701 gtgatctgga cactgagcaa gtgcttttcg agatcgccaa accggagaac gatcaggaga
     2761 atgatgagtc gccacaggag tcggcaagat acgtgcgtta tagatttgct ccaaactttt
     2821 tgaaactcaa aacggtcgga gcaactgtgg aattcaaagt aggagacatc ccaatccatc
     2881 atttccgaat gatcgaacgt cacttcttca aagatcgcct tctgaagtgt tttgactttg
     2941 aattcggatt ctgtattccg aattcacgaa acaactgtga acatatctat gagttccctc
     3001 aactctctca acaactcagt gagtcattat tctaaaaagt acaattcaaa agactaatct
     3061 ctttttcagt ggacgacatg atcaacaatc caaacgagac tcgttctgac agcttctatt
     3121 tcgtcgataa caaactcgtc atgcacaaca aagccgacta ctcatatgat gcataaatat
     3181 ttaatacaaa aatgttctgg ataattattc tgtcgaatag aaaaaaaact ccaaatgtga
     3241 ttaaattcca ataattcctg tctagtttgt tccttcccct tccccttctc atgttcaatg
     3301 cattcctaag cttttcagtt ccccccttgt ttctatattt tttcgctgtc ctgtcacact
     3361 cgctaaaaac actaatcaca cggaaatctg ttttcaataa aaactccaac tttaactcat
     3421 tttcaatttc aactgaaaga ttttttcatt agagaatgtc tagaactagg cccgggctac
     3481 gtaatacgac tcacttaagg ccttgactag agggtaccag agctcaccta ggcaggaaca
     3541 gctatgacca tgattacgcc aagctatcaa ctttgtatag aaaagttgcg agtttgttgg
     3601 agcaaaacac ggaacactag tgctagagcg tcaatttttg agctaaaaca aatctgtcac
     3661 atactgctcg aatcggaggt aggtttgaaa aacatgcaat tcatttccaa aatatgacga
     3721 attttgaaaa aaaaaaatag aaaagaaact caattaaatt accttatttt ttctagtacg
     3781 tcaattttga cttgcactta aaatctgcat tttaaaaatg aaaattcagt gcagctgcag
     3841 tgcagtgcgg ctattatttt cggcattttt gcaaaactta cacaccaccg ttataagaaa
     3901 atatttgtct ttttttttaa atttttattc agtcgttatt cgaaaaagac ttacagtacc
     3961 ttgctgaatt ccttccgcat tgcgctaaaa tttaccgatt gcaaaataat cttacatgct
     4021 atagtaataa aaaatttaca tacttgccat ttcatttcat ttaattattt catccaattt
     4081 atcttgatca gtattactct ttgttaaaaa ttccactgtt caatttttac atttcagttg
     4141 ttttaatatc gaagtgcaca agaagcgcga acataatgga caccatcgct caaaaattat
     4201 gcttctagtt gtagttcctc gagcattttt gatctcaaaa gacatttcca agagcaataa
     4261 ttgccatttt tgagctacaa gtaccatatc ctgctcataa ttcatgttcc accactccct
     4321 aaagtttttt ctttggaatt ccccagactt tgcgcctaaa attctcgtta ttctctccaa
     4381 ttcaattggc tcaattcaac tgactagatt tcagtgatgt aggtagcgcg gaactcagta
     4441 ttaagagagg actcaactca gtttctaatc caatttcaac aaattctaag acaccgaaac
     4501 gctgcaggtc tcgtagagcc tacgtgttga caggttcttc cttttcaatc tatttccgtc
     4561 gatattccgg cgtcatttag ttgaaaatga gtgaaaaaat gcgatctgac gcgaattcac
     4621 gtgaactcga aaattcgaga atgtaatcat taagtttcca aacaccaagt tctctttcga
     4681 aaccaagtca atagaaaaat gagacaccca cgagaagaag gttggtccga aaaaagaaac
     4741 taaagaagtt atttatttct ctttttgatg tttgttgagc agaatatgat ttagttaagc
     4801 acatctgatt tcaacgccga tgaatgaggt cgtaggcgtc acgttcaatt tgatctgatt
     4861 tgttcagtgc ttacgagtag aaattctaaa tatgatgtta ctttttggtt ctgtaagatg
     4921 gagctgggta tacatgtggt gcacggcaat cgctatttgg caatttcagt catttcggca
     4981 aattcggcaa ttttggcgaa ttctcatatt ttggaaattt tggcaaatgc ggaaaatttc
     5041 aaaaacaagc aattaccaaa tttgccaaac gccgaaaaat ttaaaaaaaa accgacattt
     5101 gctggtattt ttttttaatc aggatgctaa ttatgtaagg ttaatctata aattcttgaa
     5161 acacacttaa ctttgaaatt gaaatcactg acatttcgcg cattcgccga taaaatctct
     5221 aaataaaata atgtaaactg tgtccagttt tggtacttgt ttagtatacc tggttcaaaa
     5281 tattttccaa tgcgttttcc atttatttat tttagaaatg atcatttaat taaaacgtaa
     5341 aattcgcatt ttcaagctta gaaacattaa tcctaagtcc aatatggaca aaaagagtag
     5401 tgtattagta ccaagtaaga accctaaaaa aaaaatattc taattaaatt tttggcaaaa
     5461 tgccacattt ttcttttttt tctcaacggg gaaaagttgt gtttcttccc tttttattaa
     5521 attttttgtt ttccttaggc taagtacaac ttgaaatgga acagaaatta cataaattta
     5581 ttgctttcac attttgctct tcttcactca aatcttggac tcttgctttt cacaaaaaaa
     5641 accttataaa caaaaataag acatctgaaa aaatgatgaa cttcctgtca gtttgttcca
     5701 gtatccttct taaatttcct tgttttccac atcacaaaaa tcctacatca cgcttcgact
     5761 catttcctga cagtcatcac atcaccaaat gaatttctat ttttacaata tagttgtagt
     5821 atttttgtcg aacatatatt gttctaacta acttcttaaa aaattgtgct cttgactgaa
     5881 cacaaatttc tgcgtcgttt cacgtttact tttctcaatt ttttaaaacc aaaatttaac
     5941 tgcaaacttc tgcattgttc taaaaactca caggttatct caaaaagata aatttcaaag
     6001 tgagattttt ttaaaaaaat tccggactgt tttttgattt tgatgttttt aatgaatttt
     6061 taaaattctt tattagtgcc tgaaattaac aataatttta aagtttgtca cactgcgaat
     6121 aaattaatct cacatttcgt atgtttagaa agatgcatga aactccgtcg ttcttatgaa
     6181 gttaattaag acatggctaa ttgaaagccc acatggtcat gaaaaagtaa aacttacaac
     6241 tttttactga atattaacca gtctctataa atatcccagc ttagccggaa tagggtcacg
     6301 aaaacatgaa aataaagctt gaacctattg ttatgatttt caatgccacc ttttgcagtt
     6361 taatatacac tattactact tctcagttca agttcacttc tgagacggaa cctttatttt
     6421 ctaactttct ttaatcttac tttttatgag ccgaatgttc tattttgtaa aatgatcaga
     6481 tacaagccca aagctaattt tgccaataag tgttagtaga tcaattgaat tacaatataa
     6541 tgacctgggc ggaatagatt tttgcactga tttttttcca agcttactct atgaatattc
     6601 atagatcatc ttcaaaggta gcgtgtgttg tagttgtgtt ttggcgggtt cgtgttattt
     6661 gactcagctc aagtagatgt tgtagtcttc cctcaatcaa cgcgtgggct tgaccctctt
     6721 taaattgagt ggaaaccagc caatctattt gaaatctacc cccgaaatga gattcgacga
     6781 tgacacgacg tgtgtgttac gtgcgctgag aatttaggaa aaaaaagata atcttctttt
     6841 cgttagctgc gccacaacct gtgcagataa actccgcaca attgaagttc ttactggtcc
     6901 cacccgattt attcaattgt attgtgtctt tttttcttat ttctctacaa ctctaacttt
     6961 cttcaactat tctctacatc agtgcttttt tccaccatga aaaaagaaat gacgtcgttg
     7021 tttcttagga ggggcggagc ctcaactcaa tggtccgttc gaatagatcc cacccctcat
     7081 ataaatagga ctgaaaccaa agaagttttt aaattttcag cttaccgtgt ttctcaattt
     7141 tcaaaatagt cttttttgtg tctgaacggg ctgatcaatt atgttttttt ttgaatgaaa
     7201 aatcttcctc ccaagcctta aaattccttc caaagatcga aagttatgtc tattcaagtt
     7261 acttcttctt agatcaaata ttaaacaaaa aaatacgcca tcattctctc acatttagta
     7321 attcaaaatt tcgatttgtt ttcgaacttt tgaatttccc gcctaggaag tgacatgagt
     7381 gaaatctcat tagataaatg tgccactcac cgatgacact attttcaaat aagggggggg
     7441 ggtgtttcga ataccttgta cacgcgtttc cgcaaaacca cacaccccga gttcctcagc
     7501 ttcttcccct cgacccatga gccacgtcaa gatgtcagcg tgtcactttt cattgcttca
     7561 ccttctacgg taacgagaaa aggcggggaa tccaggatct ggtgtgactt cttccttctt
     7621 cgtatcttcc taaaaatgtt cgtttacttt ttagcgagtg tcatttgatg ggatgtcgtt
     7681 cttgaggaaa gaggctagaa gcttaatact ctcatatttc atattattgg catcaagacg
     7741 tttattattt aactacaata acgattgctt taacaactaa aaataataat ttcagggtta
     7801 gacatgacat gagcgtgctg acacctctgc tgctgagagg cctgacaggc tctgctagaa
     7861 ggctgcctgt gcctagagcc aagatccact ctctgggcga ccctatgtct gtgctgactc
     7921 cactgctcct gcggggactg actggatctg ctcgtagact gccagtgcca cgggctaaga
     7981 ttcacagcct gggcgatcca atgagtgtgc ttaccccact cctgctcaga ggactgaccg
     8041 gaagtgctag acgcctgcca gttccaaggg ccaaaatcca tagtctggga gatcccatgt
     8101 ccgtgttgac acccctcttg ctgaggggac ttaccggctc agctcggaga ctccctgttc
     8161 caagagcaaa gattcattcc ctcggcgacc caagatctgg cagcgctaaa gaccctacca
     8221 tgggtagcca ccaccaccat caccacggca tggcttctat gacaggcggc cagcagatgg
     8281 gccgcgacct gtacgatgac gacgataagg acctggccac aatggtggac agcagcaggc
     8341 ggaagtggaa caagacaggc cacgccgtta gagccatcgg cagactgtct agcctggaaa
     8401 acgtgtacat caaggccgac aagcagaaga acggcatcaa ggctaacttc aagatccggc
     8461 acaacatcga ggacggcgga gtgcagctgg cttaccacta ccagcagaac acccctatcg
     8521 gcgacggacc agttctgctg cctgacaacc actacctgag cgtgcagagc aagctgagca
     8581 aggaccccaa cgagaagagg gaccacatgg tgctgctgga atttgtgacc gccgctggca
     8641 tcacactcgg catggacgaa ctgtacaaag gcggcacagg cggctctatg gtgtccaagg
     8701 gcgaagaact gttcaccggc gtggtgccca ttctggtgga actggatggg gatgtgaacg
     8761 gccacaagtt cagcgtcagc ggagaaggcg aaggcgacgc cacatacgga aagctgaccc
     8821 tgaagttcat ctgcaccacc ggcaaactgc ccgtgccttg gcctacactg gtcaccacac
     8881 tgacatacgg cgtgcagtgc ttcagcagat accccgacca tatgaagcag cacgacttct
     8941 tcaagagcgc catgcctgag ggctacatcc aagagagaac catcttcttt aaggacgacg
     9001 gcaactacaa gaccagggcc gaagtgaagt tcgagggcga caccctggtc aacagaatcg
     9061 agctgaaggg catcgacttc aaagaggacg gcaacatcct gggccacaag ctcgagtaca
     9121 acctgcctga ccagctgacc gaggaacaga tcgccgagtt caaagaagag ttcagcctgt
     9181 tcgacaagga cggcgacggc acaatcacca ccaaagaact gggcaccgtg atgagaagcc
     9241 tgggacagaa ccctacagag gccgagctgc aggacatgat caacgaggtt gacgctgatg
     9301 gcgacggaac tatcgacttc cccgagttcc tgaccatgat ggccagaaag atgaagtaca
     9361 gggacacaga ggaagagatc cgcgaggcct tcggcgtgtt cgataaggat ggaaacggct
     9421 acatctctgc cgctgagctg agacacgtga tgaccaacct gggcgagaag ctgacagacg
     9481 aagaggtgga cgagatgatc agagaggccg acattgacgg cgacgggcaa gtgaactacg
     9541 aagagtttgt ccagatgatg accgccaagt gaaccggtgt cgacgaattc gggcccgttt
     9601 aaacccgctg atcagcttgt acaaagtggt tggatgatcg acgccaacgt cgttgaattt
     9661 tcaaatttta aatactgaat atttgttttt tttcctatta tttatttatt ctctttgtgt
     9721 tttttttctt gctttctaaa aaattaattc aatccaaatc taaacatgag cggttttttt
     9781 tctctttccg tctcccaatt cgtattccgc tcctctcatc tgaacacaat gtgcaagttt
     9841 atttatcttc tcgctttcat ttcattagga cgtgggggga attggtggaa gggggaaaca
     9901 cacaaaagga tgatggaaat gaaataagga cacacaatat gcaacaacat tcaattcaga
     9961 aatatggagg aaggtttaaa agaaaacata aaaatatata gaggaggaag gaaaactagt
    10021 aaaaaataag caaagaaatt aggcgaacga tgagaattgt cctcgcttgg acaactttat
    10081 tatacatagt tgataattca ctggccgtcg ttttacactc gagacgtacg gtgcgcgcga
    10141 tgcattcgaa gatctgccca ctagtgagtc gtattataag tgcaagtaag atcagtgttt
    10201 gtttcgatat cagtctgttt cgtaacggtc ttctgtataa ctacaaaaaa gaataaaaaa
    10261 ccgtatcaca actgagaaaa aagacaaaac ttattgaaca gtgttccttt caaaaaatta
    10321 ttttgtaaaa aatcgagttt ttctccaaaa taacgcacgc aatcaaaaac aagaaaacta
    10381 acgtgttctc ctgggcgtgg aactccttat caagattacc attattgtct gtcagaattg
    10441 atatttcaat tatttcatat ctagacaaac ccgggtgtct tgcatttatt agtactgtga
    10501 gtttacatac aaacgaggct tactcagata acatagagtc acctctaatt agcaaaaacc
    10561 catttctaat tagctatttc gaccagttct aaagtcaccg taattagaaa tggtttttgt
    10621 agcttttcta attagaaatg accccgagat aagtgaaata tggcattttc tgggttttct
    10681 agtaagaaat gatcccgcac gtagtgattc cgcgttacaa gccaaattca aattaaaaaa
    10741 gtatattaca caattcaagt tacattattt tcaggaaaca atggcgaaga gcaaagcaga
    10801 gattcaaaag gaggaaagag acaacagata tgaacgaaga cttcttgaaa agagaaagag
    10861 acatgaggtg agtaaccatg ataataaata atcaagtaag ggctatagat ttataaattc
    10921 tggagttaac agtttcgaaa aaaagtagtt ttcatatgtg cgaaggaata tcaaaaatag
    10981 gggaatagcc taaacgagtg agaaaattaa atgtttgtta ttttttcaaa aatgtctgca
    11041 gggccgttat cctttcaaat gcactctatc aaacatagat catatccatc atatcttatc
    11101 cagaatttat atcatatcca agattgagag cgtttcaagg aaaatctcaa acaaccatac
    11161 aattttgtag atcgacaaca aggcctccac ttcttcatcg atggcctctg cagcacctgc
    11221 accaccaagt ttgtattttt catattaata taaagtgagc cacgcatagt cgtttttagg
    11281 atttttgcca gatcgatgtc gcgcaaacag actgtttttt tttctttgaa agccgtgcgt
    11341 gacatgggcc acggattgta acgccgcgct ggatggataa aaatttattc agttttttag
    11401 cctttacaaa atatggttcg actactttct ttatttttac atttttatag gtgctcattg
    11461 agctgagata ttttgggtat aatcggaaaa tcgctgattt tggtcatttt tctgcactaa
    11521 agattacaca aaaagttcag tatttagatt cattgttgaa aattggggat gatctacaga
    11581 tacatatcgc cgtgttcaaa aacacacgtg acgtcgtgac tgggaaaacc ctggcgttac
    11641 ccaacttaat cgccttgcag cacatccccc tttcgccagc tggcgtaata gcgaagaggc
    11701 ccgcaccgat cgcccttccc aacagttgcg cagcctgaat ggcgaatggc gcctgatgcg
    11761 gtattttctc cttacgcatc tgtgcggtat ttcacaccgc atatggtgca ctctcagtac
    11821 aatctgctct gatgccgcat agttaagcca gccccgacac ccgccaacac ccgctgacgc
    11881 gccctgacgg gcttgtctgc tcccggcatc cgcttacaga caagctgtga ccgtctccgg
    11941 gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgagac gaaagggcct
    12001 cgtgatacgc ctatttttat aggttaatgt catgataata atggtttctt agacgtcagg
    12061 tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc
    12121 aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag
    12181 gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg
    12241 ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt
    12301 gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt
    12361 tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt
    12421 attatcccgt attgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa
    12481 tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag
    12541 agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac
    12601 aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac
    12661 tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac
    12721 cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac
    12781 tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact
    12841 tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg
    12901 tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt
    12961 tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat
    13021 aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta
    13081 gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa
    13141 tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga
    13201 aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac
    13261 aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt
    13321 tccgaaggta actggcttca gcagagcgca gataccaaat actgtccttc tagtgtagcc
    13381 gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat
    13441 cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag
    13501 acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc
    13561 cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag
    13621 cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac
    13681 aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg
    13741 gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct
    13801 atggaaaaac gccagcaacg cggccttttt acggttcctg gtcttttgct ggccttttgc
    13861 tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga
    13921 gtgagctgat accagcggat aacaatttca ca
//
