LOCUS       Exported                8375 bp DNA     circular SYN 29-NOV-2022
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8375)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Nov 29, 2022 from SnapGene 6.0.2
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..8375
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        43..3157
                     /locus_tag="rig-3"
                     /label=rig-3
     primer_bind     43..63
                     /locus_tag="OAS-788"
                     /label=OAS-788
     primer_bind     complement(3131..3154)
                     /locus_tag="OAS-786"
                     /label=OAS-786
     promoter        complement(3158..3181)
                     /locus_tag="rig-3(1)"
                     /label=rig-3
                     /label=rig-3(1)
     CDS             3182..4568
                     /locus_tag="GCaMP6"
                     /label=GCaMP6
     misc_feature    3191..3208
                     /locus_tag="6xHis tag"
                     /label=6xHis tag
     misc_feature    3248..3279
                     /locus_tag="Xpress tag"
                     /label=Xpress tag
     primer_bind     3653..3670
                     /locus_tag="OAS-967"
                     /label=OAS-967
     3'UTR           4569..5443
                     /locus_tag="unc-54"
                     /label=unc-54
                     /note="/vntifkey=50"
     CDS             4569
                     /locus_tag="gfp"
                     /label=gfp
                     /note="/vntifkey=4"
     primer_bind     complement(5498..5523)
                     /locus_tag="SP6"
                     /label=SP6
     primer_bind     complement(5529..5549)
                     /locus_tag="M13-rev"
                     /label=M13-rev
     misc_binding    complement(5555..5577)
                     /locus_tag="LacO"
                     /label=LacO
     rep_origin      complement(5917..6599)
                     /direction=LEFT
                     /locus_tag="ColE1 origin"
                     /label=ColE1 origin
     CDS             complement(6697..7356)
                     /locus_tag="AmpR"
                     /label=AmpR
     CDS             complement(8196..8264)
                     /locus_tag="LacZ alpha"
                     /label=LacZ alpha
     primer_bind     8335..8352
                     /locus_tag="M13-fwd"
                     /label=M13-fwd
     primer_bind     join(8351..8375,1..3)
                     /locus_tag="T7"
                     /label=T7
ORIGIN
        1 gggcgaattg ggcccgacgt cgcatgctcc cggccgccat ggacaccacg ctcacacacg
       61 atgaaattca tacccaccaa cctcccttct ctgcctccct caacttcttc cacggaattc
      121 tatctcctct tatccagtta gggacacata tcgagtttca ccaaaaataa tgagaagtgt
      181 ggtcagagac accacattga attaactatt agttcaaaag ttgcaggttt cctagtgcaa
      241 aaagtttatg ttcccatcta ccaaacgact caccgccaat tgactttgca gtggcaacat
      301 tgggaaatgc gacccatcat attgttccta ttgtcatgta caagctgtcc ctgttttgct
      361 cgcttaatcc gtcaacccct tccccgttct aattagagat gagtgaatta caatagagtt
      421 gaaacggaaa tctgagtgtc tgtagctttc cgccgccgct tttgttacga gatggtagac
      481 gtccctttct tggtgtttat tgtctcacaa actgtataat aaagcaccgt tgtgtaaaag
      541 gcatttggta tcaggaagat aaaatgaata aaatgcaaat gcggaatcaa aaattgccaa
      601 ataattttga ccaataaaat atcaaaaata atagaacaga aataaacaaa taaaactacg
      661 gagaaaatgt gattattcca ttaaacaacg ttttaaacca actttattcg tcagtgggga
      721 aggttttgga caaatatcat agtaaaactt gttcgtttta attatacata atcaataatt
      781 ataggtgtta gaaccaatgg tcgaaattta aaaaaaaatc aacattttag aaaatttcgg
      841 caatttaccg aaatattgac tgacaatttt acatatataa gtgttttgaa atagaatccc
      901 cattgacgat gcgccacctt aaacagtaga actgttccaa tcagaaacta aataaatacg
      961 gttattatat atatatatat atatatatat atataacttg tttttattat gcatttctca
     1021 aaactcacca aactcaatct ctaatttacg gttgttgagg aaaatatatt tatcacattt
     1081 agttgagact ctaaaagaag tacaactttt ttcttgcaat tcctccacca atccaatttt
     1141 tcaagagtca attgcttgga gtcctcgttt tctcgtaatc cgatattccc cctatttctg
     1201 tgattttatt cagtttcgtc ctcttacacc aaaaatcttc atgacttttt tggttttctg
     1261 cttaatcttt tttttgtttt taaagtctac catccggcaa ttttttttgc aaatggttgc
     1321 tcagaatttg acctcagatt aaaattttca ggaacttcct ggtcccattt cgtcacattt
     1381 aataacaaac caaatccttc agcttgagtc gtccaagcca acgcaaccct aattcgattc
     1441 cacctccaat ttaccttttt gccgttttcg atcaaattgt gggggctctt tttatttgaa
     1501 agtcgtaaaa tggcgcgtcc ttgtcgaaaa ttgagatagc taatggttcc aaataaaatc
     1561 aaatgcatga acctagtagg aaaaatcgtt ttttttttca tcttccaaag aaaaagttta
     1621 agataaaccc accactccag caatccacct acgcgttttt caccggaaac tgtcatttgt
     1681 ttttgatgta tgacagtgcc agcactgaac taaattccca tttgttttgt tgtggtgtta
     1741 tctaaccacg tggctctttc tctaaatttt gcgtaggctg caagatccgc aatcccacca
     1801 aaaaatggtt tctaatgtac ttcatacata tcccacatga ctcagtgctc cgaaacgccc
     1861 agcaaccccc gcgtttgtct ttacaaaaat gaggcggaaa tgggaaattt tcctgctatt
     1921 gctatggtaa aatacttaag ctgtattgag atttgggaaa ttgaaagata agatgaaaaa
     1981 tccactctgt ataattaaaa aggtttattt tttttgtaat ttttgtaccg caaatttttt
     2041 cattaactga tttttcttta ttcattcgat ggcccagttt cagatcttca ttatcatgta
     2101 gtttattcat tcaaactatt tgtgacatca tgatggttgt ttaaaaataa tttcgaatga
     2161 agaaagctta aacctattat aattttcttt tctataaaaa atattgattt tgtaaagcgt
     2221 ttggcagtga cagtaccaaa aaatgtttta aaatgattta agaaaatttt ttattttatt
     2281 tgtataatac gcgtaaaatg agtatactcg tcatttgtca cgaatcgagt tctcagtttg
     2341 ctgtgaatat cttgtaatag aaatcgatct catagtgtca cttttgaggc ataacttttc
     2401 aggttgttac aagttcatta acttgtcaag actgggtcat gttttaaagt gtaactgtta
     2461 cccatgtacc ctaaactttg agacaaactc ctcaaaactt gtaaccagaa tcctatgtag
     2521 atgactcaca aaaccctgtt ttcaatttga acgaatttcg aaactggcca aaatgttccc
     2581 ctcttgctca cctaatgctt ttaccattta aaaaggatgg taacaacact ctttcgaaaa
     2641 tgtaggcggt ccattttcgg tttctcataa ccgtctggct ctatctccta gtgcgcgagg
     2701 agagggcggg cggagtagga atcgggggca gcgcactcat acactctgcc cttctctttt
     2761 gcacacacag aaaaagatag atacagacca acacttattg ttgtccagta agcttccaga
     2821 aaactatttt cagaattaca aactacattt ttctagaaca gtcaattgat tttttacaag
     2881 attcgagtat tctaacatta gtgaaagtat ttagccactg caattttcaa ctgtaggttt
     2941 caacttccaa aaaaaattcc tagcatttct aattggatga aaaaaggcta tctagaccct
     3001 ccattattta ctcataattt catctcgtcc tcatttttaa tcccctcatt gatcccatca
     3061 caatccgcca aaatgttttt taaacacttt tttcttcacc agccgtatgt ggtcaactgc
     3121 ctaaaaattt cacagctatt tgtcattcct tgcagaagaa cttcattctt ctttttcgaa
     3181 aatgggttct catcatcatc atcatcatgg tatggctagc atgactggtg gacagcaaat
     3241 gggtcgggat ctgtacgacg atgacgataa ggatctcgcc accatggtcg actcatcacg
     3301 tcgtaagtgg aataagacag gtcacgcagt cagagctata ggtcggctga gctcactcga
     3361 gaacgtctat atcaaggccg acaagcagaa gaacggcatc aaggcgaact tcaagatccg
     3421 ccacaacatc gaggacggcg gcgtgcagct cgcctaccac taccagcaga acacccccat
     3481 cggcgacggc cccgtgctgc tgcccgacaa ccactacctg agcgtgcagt ccaaactttc
     3541 gaaagacccc aacgagaagc gcgatcacat ggtcctgctg gagttcgtga ccgccgccgg
     3601 gatcactctc ggcatggacg agctgtacaa gggcggtacc ggagggagca tggtgagcaa
     3661 gggcgaggag ctgttcaccg gggtggtgcc catcctggtc gagctggacg gcgacgtaaa
     3721 cggccacaag ttcagcgtgt ccggcgaggg tgagggcgat gccacctacg gcaagctgac
     3781 cctgaagttc atctgcacca ccggcaagct gcccgtgccc tggcccaccc tcgtgaccac
     3841 cctgacctac ggcgtgcagt gcttcagccg ctaccccgac cacatgaagc agcacgactt
     3901 cttcaagtcc gccatgcccg aaggctacat ccaggagcgc accatcttct tcaaggacga
     3961 cggcaactac aagacccgcg ccgaggtgaa gttcgagggc gacaccctgg tgaaccgcat
     4021 cgagctgaag ggcatcgact tcaaggagga cggcaacatc ctggggcaca agctggagta
     4081 caacctgccg gaccaactga ctgaagagca gatcgcagaa tttaaagagg aattctccct
     4141 atttgacaag gacggggatg ggacaataac aaccaaggag ctggggacgg tgatgcggtc
     4201 tctggggcag aaccccacag aagcagagct gcaggacatg atcaatgaag tagatgccga
     4261 cggtgacggc acaatcgact tccctgagtt cctgacaatg atggcaagaa aaatgaaata
     4321 cagggacacg gaagaagaaa ttagagaagc gttcggtgtg tttgataagg atggcaatgg
     4381 ctacatcagt gcagcagagc ttcgccacgt gatgacaaac cttggagaga agttaacaga
     4441 tgaagaggtt gatgaaatga tcagggaagc agacatcgat ggggatggtc aggtaaacta
     4501 cgaagagttt gtacaaatga tgacagcgaa gtacaagttc tgctacgaga acgaggtgta
     4561 atgacaatca ttcgtagaat tccaactgag cgccggtcgc taccattacc aacttgtctg
     4621 gtgtcaaaaa taataggggc cgctgtcatc agagtaagtt taaactgagt tctactaact
     4681 aacgagtaat atttaaattt tcagcatctc gcgcccgtgc ctctgacttc taagtccaat
     4741 tactcttcaa catccctaca tgctctttct ccctgtgctc ccacccccta tttttgttat
     4801 tatcaaaaaa acttcttctt aatttctttg ttttttagct tcttttaagt cacctctaac
     4861 aatgaaattg tgtagattca aaaatagaat taattcgtaa taaaaagtcg aaaaaaattg
     4921 tgctccctcc ccccattaat aataattcta tcccaaaatc tacacaatgt tctgtgtaca
     4981 cttcttatgt tttttttact tctgataaat tttttttgaa acatcataga aaaaaccgca
     5041 cacaaaatac cttatcatat gttacgtttc agtttatgac cgcaattttt atttcttcgc
     5101 acgtctgggc ctctcatgac gtcaaatcat gctcatcgtg aaaaagtttt ggagtatttt
     5161 tggaattttt caatcaagtg aaagtttatg aaattaattt tcctgctttt gctttttggg
     5221 ggtttcccct attgtttgtc aagagtttcg aggacggcgt ttttcttgct aaaatcacaa
     5281 gtattgatga gcacgatgca agaaagatcg gaagaaggtt tgggtttgag gctcagtgga
     5341 aggtgagtag aagttgataa tttgaaagtg gagtagtgtc tatggggttt ttgccttaaa
     5401 tgacagaata cattcccaat ataccaaaca taactgtttc ctaggtcgac catatgggag
     5461 aggcctccca acgcgttgga tgcatagctt gagtattcta tagtgtcacc taaatagctt
     5521 ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt tatccgctca caattccaca
     5581 caacatacga gccggaagca taaagtgtaa agcctggggt gcctaatgag tgagctaact
     5641 cacattaatt gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt cgtgccagct
     5701 gcattaatga atcggccaac gcgcggggag aggcggtttg cgtattgggc gctcttccgc
     5761 ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca
     5821 ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg
     5881 agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcga
     5941 taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa
     6001 cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc
     6061 tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc
     6121 gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct
     6181 gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg
     6241 tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag
     6301 gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta
     6361 cggctacact agaaggacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg
     6421 aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt
     6481 tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt
     6541 ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag
     6601 attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat
     6661 ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc
     6721 tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat
     6781 aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc
     6841 acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag
     6901 aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag
     6961 agtaagtagt tcgccagtta atagtttgcg caacgttgtt ggcattgcta caggcatcgt
     7021 ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg
     7081 agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt
     7141 tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc
     7201 tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc
     7261 attctgagaa taccgcgccc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa
     7321 tagtgtatga catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg
     7381 aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc
     7441 caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag
     7501 gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt
     7561 cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt
     7621 tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc
     7681 acctgtatgc ggtgtgaaat accgcacaga tgcgtaagga gaaaataccg catcaggcga
     7741 aattgtaaac gttaatattt tgttaaaatt cgcgttaaat atttgttaaa tcagctcatt
     7801 ttttaaccaa taggccgaaa tcggcaaaat cccttataaa tcaaaagaat agaccgagat
     7861 agggttgagt gttgttccag tttggaacaa gagtccacta ttaaagaacg tggactccaa
     7921 cgtcaaaggg cgaaaaaccg tctatcaggg cgatggccca ctacgtgaac catcacccaa
     7981 atcaagtttt ttgcggtcga ggtgccgtaa agctctaaat cggaacccta aagggagccc
     8041 ccgatttaga gcttgacggg gaaagccggc gaacgtggcg agaaaggaag ggaagaaagc
     8101 gaaaggagcg ggcgctaggg cgctggcaag tgtagcggtc acgctgcgcg taaccaccac
     8161 acccgccgcg cttaatgcgc cgctacaggg cgcgtccatt cgccattcag gctgcgcaac
     8221 tgttgggaag ggcgatcggt gcgggcctct tcgctattac gccagctggc gaaaggggga
     8281 tgtgctgcaa ggcgattaag ttgggtaacg ccagggtttt cccagtcacg acgttgtaaa
     8341 acgacggcca gtgaattgta atacgactca ctata
//
